##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295126_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3241674 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.69994175848651 33.0 27.0 33.0 15.0 33.0 2 22.872627229018093 33.0 6.0 33.0 2.0 33.0 3 25.207751920766864 33.0 15.0 33.0 15.0 33.0 4 25.890960041015845 33.0 15.0 37.0 2.0 37.0 5 27.732106621455458 37.0 15.0 37.0 2.0 37.0 6 28.285954417378182 33.0 22.0 37.0 6.0 37.0 7 28.102592055832883 33.0 15.0 37.0 6.0 37.0 8 28.680679179954556 33.0 27.0 37.0 6.0 37.0 9 29.752958502304672 37.0 27.0 37.0 15.0 37.0 10-11 29.249021955940048 35.0 24.5 37.0 10.5 37.0 12-13 28.12785616320457 33.0 18.5 37.0 4.0 37.0 14-15 29.891176133071987 35.0 27.0 37.0 10.5 40.0 16-17 30.296081129687934 37.0 27.0 40.0 10.5 40.0 18-19 30.403741246035228 37.0 27.0 40.0 10.5 40.0 20-21 29.952453269514457 35.0 27.0 40.0 6.0 40.0 22-23 30.686762919405222 37.0 27.0 40.0 15.0 40.0 24-25 30.801908211621527 37.0 27.0 40.0 15.0 40.0 26-27 29.392814021397584 35.0 24.5 40.0 4.0 40.0 28-29 30.076895301625026 37.0 27.0 40.0 10.5 40.0 30-31 30.736328822700862 37.0 27.0 40.0 15.0 40.0 32-33 29.527561839962935 33.0 27.0 40.0 6.0 40.0 34-35 29.228990484545946 33.0 22.0 37.0 6.0 40.0 36-37 29.55491360328028 33.0 27.0 37.0 6.0 40.0 38-39 29.64612434809916 33.0 24.5 37.0 6.0 40.0 40-41 28.79422144237823 33.0 22.0 37.0 6.0 40.0 42-43 29.48531900493387 33.0 22.0 37.0 6.0 40.0 44-45 29.3286514930249 33.0 22.0 37.0 6.0 40.0 46-47 28.97022603136528 33.0 22.0 37.0 6.0 40.0 48-49 28.880929112551108 33.0 22.0 37.0 6.0 40.0 50-51 28.852464189798233 33.0 22.0 37.0 6.0 40.0 52-53 29.058530098955046 33.0 22.0 37.0 6.0 40.0 54-55 29.033121467488712 33.0 22.0 37.0 6.0 40.0 56-57 29.02278313612041 33.0 22.0 37.0 6.0 40.0 58-59 28.88662817420876 33.0 22.0 37.0 6.0 40.0 60-61 28.878882022066378 33.0 22.0 37.0 10.5 40.0 62-63 28.781413399373285 33.0 22.0 37.0 10.5 40.0 64-65 28.34397552005538 33.0 22.0 37.0 6.0 40.0 66-67 28.578030517565924 33.0 22.0 37.0 10.5 40.0 68-69 28.19812556722237 33.0 22.0 37.0 6.0 40.0 70-71 28.318470025054957 33.0 22.0 37.0 10.5 38.5 72-73 28.334379397804963 33.0 22.0 37.0 10.5 37.0 74-75 28.401519400161767 33.0 22.0 37.0 15.0 37.0 76-77 28.15545517531991 33.0 22.0 37.0 15.0 37.0 78-79 27.70174607317084 33.0 22.0 37.0 10.5 37.0 80-81 27.53247134042473 33.0 22.0 37.0 10.5 37.0 82-83 27.624741877190612 33.0 22.0 35.0 15.0 37.0 84-85 27.55361797639121 33.0 22.0 33.0 15.0 37.0 86-87 27.317597173559093 27.0 22.0 33.0 15.0 37.0 88-89 27.22564668748307 27.0 22.0 33.0 15.0 37.0 90-91 27.379864539123922 27.0 22.0 33.0 15.0 37.0 92-93 27.332289736722448 27.0 22.0 33.0 15.0 37.0 94-95 27.209243588343554 27.0 22.0 33.0 15.0 37.0 96-97 27.280894377411176 27.0 22.0 33.0 15.0 37.0 98-99 27.3184806677044 27.0 22.0 33.0 15.0 37.0 100 26.974119235925635 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.329932730748926 1101 2 11.316767452546898 1101 3 8.433470138833936 1101 4 12.853077071187617 1101 5 10.793974882186138 1101 6 10.053615220934844 1101 7 9.504933210368186 1101 8 9.138551828066927 1101 9 7.318094778590616 1101 10-11 7.853085437755343 1101 12-13 9.101635155243155 1101 14-15 8.033170734930934 1101 16-17 8.287878316583562 1101 18-19 8.26012373674261 1101 20-21 7.29530526827228 1101 22-23 7.389500329984564 1101 24-25 7.403429593809516 1101 26-27 8.557394826873963 1101 28-29 8.238798881648243 1101 30-31 5.494144141172846 1101 32-33 7.123076208617327 1101 34-35 8.796865326128334 1101 36-37 8.258880891060826 1101 38-39 8.257621022317807 1101 40-41 9.032915472602145 1101 42-43 8.613922605114698 1101 44-45 8.563468079018755 1101 46-47 7.458250734963979 1101 48-49 5.577094041572259 1101 50-51 6.95966369938656 1101 52-53 6.058835745914987 1101 54-55 4.048326372903343 1101 56-57 6.223850189064056 1101 58-59 6.400333652841134 1101 60-61 6.911703212164252 1101 62-63 6.472686589204631 1101 64-65 6.836492829639823 1101 66-67 5.92387383305563 1101 68-69 6.828773614993352 1101 70-71 6.368140409861567 1101 72-73 5.620271539671748 1101 74-75 4.934217756105497 1101 76-77 5.137555312710745 1101 78-79 5.335210208319797 1101 80-81 5.01793569919791 1101 82-83 4.603928544470264 1101 84-85 4.293126291023299 1101 86-87 4.202183727501549 1101 88-89 4.188801313588467 1101 90-91 3.9577053308836376 1101 92-93 3.9196461655644015 1101 94-95 3.831196652331858 1101 96-97 3.9211498274904812 1101 98-99 3.656939499450157 1101 100 3.971459511689858 1102 1 6.811313817401967 1102 2 10.68119013457406 1102 3 8.21112299462921 1102 4 12.743082396617073 1102 5 10.63985691345938 1102 6 9.56350370776908 1102 7 6.8348557980354165 1102 8 7.926905803668102 1102 9 6.587067979203621 1102 10-11 7.566956513432292 1102 12-13 9.119652915984286 1102 14-15 6.325040666183817 1102 16-17 7.455022804971893 1102 18-19 7.558538165411466 1102 20-21 7.074738757574174 1102 22-23 6.658460404795804 1102 24-25 7.01862559520902 1102 26-27 8.578285789161495 1102 28-29 7.79389639432339 1102 30-31 4.457916764221409 1102 32-33 4.461758819657234 1102 34-35 5.076565688502555 1102 36-37 5.326097529429234 1102 38-39 4.739758957285002 1102 40-41 6.402441138211557 1102 42-43 7.057832234917495 1102 44-45 6.704779075348782 1102 46-47 5.711440815241385 1102 48-49 3.9482763838978343 1102 50-51 5.248848975766361 1102 52-53 6.049211753101506 1102 54-55 5.5792836766390685 1102 56-57 6.5572347251778105 1102 58-59 6.2800706817779925 1102 60-61 6.226582847103618 1102 62-63 4.528059916904571 1102 64-65 6.365554120425344 1102 66-67 3.9405071133947587 1102 68-69 6.269004438051127 1102 70-71 5.590323208089998 1102 72-73 5.21851822059401 1102 74-75 4.411363265024914 1102 76-77 3.4026011093623296 1102 78-79 4.494657954430043 1102 80-81 4.613522737191566 1102 82-83 4.029855506821377 1102 84-85 3.079130884835152 1102 86-87 3.3578471317744913 1102 88-89 3.6169409215120183 1102 90-91 3.4718374532353593 1102 92-93 3.4033484682238075 1102 94-95 3.2251877003892098 1102 96-97 3.462313207772379 1102 98-99 3.3004032913426116 1102 100 3.5379637927430245 1103 1 6.645351909922432 1103 2 10.97357095355995 1103 3 7.93268657820963 1103 4 11.953807870640258 1103 5 10.327774582275108 1103 6 9.013722096808124 1103 7 8.093699401206635 1103 8 8.099157574305753 1103 9 6.995466083578965 1103 10-11 7.386507277399065 1103 12-13 8.75904539869013 1103 14-15 8.548688019265548 1103 16-17 8.02282331351292 1103 18-19 7.321722211456407 1103 20-21 8.355669916528193 1103 22-23 6.955273936754011 1103 24-25 5.246929063805755 1103 26-27 6.033180302974994 1103 28-29 7.039475909749854 1103 30-31 6.1575533136456855 1103 32-33 8.016267818630531 1103 34-35 7.158050479236934 1103 36-37 4.272118132476983 1103 38-39 6.501802205145481 1103 40-41 6.783803768034684 1103 42-43 7.520346295245101 1103 44-45 5.770166528737512 1103 46-47 6.470103579835172 1103 48-49 5.770702756303194 1103 50-51 6.534266255445814 1103 52-53 6.247354601050926 1103 54-55 4.9732779486948395 1103 56-57 5.966499726523356 1103 58-59 5.553008887497157 1103 60-61 5.146557035345399 1103 62-63 2.9525028925943637 1103 64-65 5.212799806700325 1103 66-67 4.243063069717735 1103 68-69 5.253992262705793 1103 70-71 3.8563570631229673 1103 72-73 4.596491052495175 1103 74-75 3.4343799460153903 1103 76-77 3.6936711446981576 1103 78-79 3.023355941101336 1103 80-81 4.21391670701615 1103 82-83 3.5391650618693404 1103 84-85 3.4364184221639356 1103 86-87 2.9242452205974416 1103 88-89 3.268150792667118 1103 90-91 2.824791605942707 1103 92-93 2.3975627153274957 1103 94-95 2.876757747538523 1103 96-97 3.061855292233343 1103 98-99 2.9524752389706386 1103 100 3.074277959971912 1104 1 6.346265186290442 1104 2 9.75954655344366 1104 3 7.326419959773929 1104 4 12.282847594196394 1104 5 9.626539546299082 1104 6 8.211920155485835 1104 7 3.916393419139599 1104 8 6.579738691335553 1104 9 6.611126020202288 1104 10-11 7.217910541405995 1104 12-13 7.4097543209877905 1104 14-15 7.246533506504285 1104 16-17 7.200336122016726 1104 18-19 6.94690496347479 1104 20-21 7.545329549770564 1104 22-23 6.550490513675079 1104 24-25 6.0628251870495795 1104 26-27 8.3984502687418 1104 28-29 7.278250866738382 1104 30-31 6.301686939847741 1104 32-33 7.65486483753309 1104 34-35 7.923481808055982 1104 36-37 6.59054328966441 1104 38-39 7.265344566104929 1104 40-41 7.149122724517586 1104 42-43 7.4283847566291605 1104 44-45 6.824302221301373 1104 46-47 6.834142166331059 1104 48-49 5.564516709045591 1104 50-51 5.750785063057826 1104 52-53 6.110465046539392 1104 54-55 4.970243444714235 1104 56-57 6.0802843936620725 1104 58-59 3.9576551746220616 1104 60-61 4.524373418864432 1104 62-63 3.911244243283438 1104 64-65 2.788206554736739 1104 66-67 3.1922410521141416 1104 68-69 4.182284665706888 1104 70-71 2.5466901807766575 1104 72-73 2.432573177221201 1104 74-75 3.1412514542496623 1104 76-77 3.854086554768813 1104 78-79 4.320172130541035 1104 80-81 3.204251485597805 1104 82-83 3.0701129823789657 1104 84-85 3.041833401211413 1104 86-87 0.8519869013602772 1104 88-89 0.3783912335843276 1104 90-91 2.3149543652779165 1104 92-93 2.5596391770311477 1104 94-95 2.468806384988749 1104 96-97 2.8360092135292163 1104 98-99 2.5091510668695065 1104 100 0.7968051031592545 1105 1 5.757961324584382 1105 2 -2.8065654427926283 1105 3 0.37671162284187787 1105 4 8.610446199675739 1105 5 8.686129653938526 1105 6 8.086615616018094 1105 7 5.337992816090804 1105 8 5.914232146758529 1105 9 4.9458622130648635 1105 10-11 5.0265106247347475 1105 12-13 5.104379804287166 1105 14-15 5.736750295468589 1105 16-17 6.116291212948202 1105 18-19 6.207951656581155 1105 20-21 7.0560282435854305 1105 22-23 1.8833103961191604 1105 24-25 4.0592405784719325 1105 26-27 6.937928723668794 1105 28-29 5.946318034742042 1105 30-31 5.233745337444049 1105 32-33 7.108762893897001 1105 34-35 6.109464513578228 1105 36-37 6.091479594100434 1105 38-39 4.062307277035252 1105 40-41 5.388829114840121 1105 42-43 5.584627291347193 1105 44-45 6.240794463113744 1105 46-47 5.399699234299437 1105 48-49 5.19480427785782 1105 50-51 6.047190471572705 1105 52-53 5.649550024804359 1105 54-55 5.350291717643479 1105 56-57 5.162837936002202 1105 58-59 1.8494924973433626 1105 60-61 4.302272299440464 1105 62-63 -0.8788457508851586 1105 64-65 -0.4336548236515867 1105 66-67 0.850165651217786 1105 68-69 2.8954890214328373 1105 70-71 2.8788467474591 1105 72-73 2.6530026574477397 1105 74-75 2.6206863010643957 1105 76-77 1.8764855933309583 1105 78-79 3.212140711072493 1105 80-81 1.6517664931300722 1105 82-83 0.9607314388143067 1105 84-85 1.3138009719643122 1105 86-87 -0.3313959785813978 1105 88-89 1.5629135798938343 1105 90-91 1.2587896451601388 1105 92-93 1.5712719638784804 1105 94-95 0.9568688456705239 1105 96-97 1.9629773315805643 1105 98-99 1.9464034387338423 1105 100 2.0396248529191254 1106 1 6.121468954207753 1106 2 10.289371395587867 1106 3 6.698497491025147 1106 4 11.743377948974025 1106 5 9.908171440665843 1106 6 9.336414628426809 1106 7 6.501893895902551 1106 8 7.4688855016698135 1106 9 6.826162687926189 1106 10-11 5.7248003406628705 1106 12-13 7.872906572114946 1106 14-15 7.809634626311237 1106 16-17 7.738536881063492 1106 18-19 7.247668755302502 1106 20-21 8.22995181235843 1106 22-23 6.187866240603626 1106 24-25 5.033126921247334 1106 26-27 7.782237239815732 1106 28-29 6.302958901911204 1106 30-31 5.335278549215801 1106 32-33 7.3546366554719285 1106 34-35 6.812166231538022 1106 36-37 5.963244610454979 1106 38-39 7.142201913263207 1106 40-41 6.818154732823057 1106 42-43 6.292142209281376 1106 44-45 4.477085407042921 1106 46-47 6.05292112382298 1106 48-49 5.867278474395121 1106 50-51 6.62998248736303 1106 52-53 5.713988061717142 1106 54-55 5.384743196445616 1106 56-57 4.922345280814561 1106 58-59 5.085185023456113 1106 60-61 4.961552271264193 1106 62-63 5.021514858075822 1106 64-65 5.34446847726181 1106 66-67 3.0317176016737157 1106 68-69 3.3689746401922562 1106 70-71 3.0744359624712985 1106 72-73 3.005229930191387 1106 74-75 2.983981763164678 1106 76-77 3.529001635324583 1106 78-79 4.2533471909249485 1106 80-81 3.752571573336585 1106 82-83 2.258539637591465 1106 84-85 2.7787292557586234 1106 86-87 3.365900130978634 1106 88-89 3.353966483786099 1106 90-91 3.0849766908883254 1106 92-93 2.9604460724113792 1106 94-95 1.5731873964010354 1106 96-97 1.5759230783999136 1106 98-99 1.9736804628849036 1106 100 2.9048543626538574 1107 1 6.1643824374362595 1107 2 10.48007250868697 1107 3 6.236117751753831 1107 4 11.455760495183444 1107 5 9.506991606613585 1107 6 8.106817364786004 1107 7 3.816624909753777 1107 8 6.4568729176985045 1107 9 5.732405926421659 1107 10-11 4.68348941441462 1107 12-13 6.334487308660183 1107 14-15 6.336217928475861 1107 16-17 7.234889576889717 1107 18-19 6.44664711950163 1107 20-21 5.4587667979459695 1107 22-23 5.750278869782228 1107 24-25 4.610935167298539 1107 26-27 7.251226616568662 1107 28-29 6.539213862102194 1107 30-31 5.125004530071081 1107 32-33 5.070009974730027 1107 34-35 6.708735007018291 1107 36-37 6.585556616360776 1107 38-39 6.43451357142056 1107 40-41 7.8163049216325255 1107 42-43 6.254398024185019 1107 44-45 4.31140958952415 1107 46-47 3.663524513762116 1107 48-49 4.190100682234387 1107 50-51 4.738419262212993 1107 52-53 3.873062438403405 1107 54-55 5.390946480906262 1107 56-57 5.367081240724627 1107 58-59 3.114728212604053 1107 60-61 2.941063572425808 1107 62-63 0.48231468105159436 1107 64-65 2.1541687563104404 1107 66-67 4.09767655347882 1107 68-69 5.161267497712156 1107 70-71 4.368088391646211 1107 72-73 2.466335017431412 1107 74-75 2.507557080292152 1107 76-77 3.072668186906789 1107 78-79 4.024939718169595 1107 80-81 2.2846206369771203 1107 82-83 1.9792259546181228 1107 84-85 2.3460625936142456 1107 86-87 2.390973748777988 1107 88-89 2.7015087619266893 1107 90-91 1.5368243890782338 1107 92-93 2.387423470633948 1107 94-95 2.432900031825074 1107 96-97 2.4297604415217187 1107 98-99 1.882231608766702 1107 100 0.24005842276505973 1108 1 4.314554457061419 1108 2 -3.157529316364119 1108 3 0.08895474293429473 1108 4 6.8226360876179655 1108 5 7.10645036878093 1108 6 6.808141313862425 1108 7 4.417084794156338 1108 8 5.066918992368066 1108 9 4.885337048647468 1108 10-11 3.264152618112387 1108 12-13 2.450704971494819 1108 14-15 3.9012633979724782 1108 16-17 4.655355663531047 1108 18-19 4.054606680152343 1108 20-21 4.612670586736222 1108 22-23 4.0746862941567805 1108 24-25 2.91211300473654 1108 26-27 4.780124330112397 1108 28-29 6.172565327106732 1108 30-31 4.418361800659582 1108 32-33 4.591164531823388 1108 34-35 3.0780101258501134 1108 36-37 1.2254665647454814 1108 38-39 2.576275294137851 1108 40-41 5.446605476228061 1108 42-43 3.008705256377958 1108 44-45 3.7002709503605544 1108 46-47 4.711538049254571 1108 48-49 4.761827433481027 1108 50-51 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-3.7720860783908456 2212 90-91 -4.446233951032397 2212 92-93 -5.931618226167934 2212 94-95 -4.178479767593593 2212 96-97 -5.81850626255763 2212 98-99 -4.083961514035263 2212 100 -4.059090905523895 2213 1 -21.281387011740346 2213 2 -17.1888655946634 2213 3 -20.13412642768967 2213 4 -19.286005546838137 2213 5 -21.51061202768511 2213 6 -22.181229199666014 2213 7 -10.925577753361253 2213 8 -10.590492644817012 2213 9 -3.9178582791424823 2213 10-11 -17.964241699232957 2213 12-13 -16.55327162658417 2213 14-15 -24.030335166356622 2213 16-17 -18.30425975956443 2213 18-19 -18.82467671477806 2213 20-21 -18.03277474924917 2213 22-23 -20.294695784522006 2213 24-25 -7.546897392616739 2213 26-27 -23.69709819887711 2213 28-29 -24.6182960754333 2213 30-31 -19.91731679459507 2213 32-33 -23.87377196734834 2213 34-35 -18.39473237085149 2213 36-37 -24.306905053145186 2213 38-39 -11.791349075398614 2213 40-41 -17.70777491227414 2213 42-43 -23.894594025786592 2213 44-45 -17.72286576832126 2213 46-47 -17.552030088064402 2213 48-49 -6.31277944081198 2213 50-51 -24.065953253403137 2213 52-53 -18.445458012626514 2213 54-55 -18.32641557244098 2213 56-57 -18.263499497071987 2213 58-59 -18.71323645447154 2213 60-61 -19.994802929443487 2213 62-63 -18.15570136699008 2213 64-65 -18.242542936290796 2213 66-67 -9.04856250691346 2213 68-69 -23.48211694294656 2213 70-71 -18.094414623874254 2213 72-73 -9.636005015501794 2213 74-75 -4.498772776271963 2213 76-77 -3.2323653899010907 2213 78-79 -4.754667110501121 2213 80-81 -3.4373732776916412 2213 82-83 -3.300006150484421 2213 84-85 -3.1158293275054305 2213 86-87 -3.3955707471692804 2213 88-89 -2.8467804473918044 2213 90-91 -2.7441600563089956 2213 92-93 -3.8833930885247625 2213 94-95 -3.742168115653687 2213 96-97 -3.5594535726471754 2213 98-99 -3.9180113403882295 2213 100 -3.2972279678353615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 832.0 3 777.0 4 3818.0 5 14267.0 6 23144.0 7 26194.0 8 23692.0 9 23817.0 10 28349.0 11 35017.0 12 43373.0 13 58678.0 14 44819.0 15 49008.0 16 56598.0 17 60336.0 18 60908.0 19 61880.0 20 61183.0 21 59276.0 22 61541.0 23 67831.0 24 74776.0 25 82110.0 26 90924.0 27 98433.0 28 107574.0 29 114937.0 30 123958.0 31 136256.0 32 154574.0 33 188570.0 34 232370.0 35 275974.0 36 311606.0 37 285312.0 38 98651.0 39 311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.137306798854965 21.887447196738904 14.325480600443278 23.64976540396285 2 14.479297752090838 21.9736133537808 41.062884074797466 22.48420481933089 3 17.079147572958064 28.059723110640395 30.36686136132462 24.494267955076918 4 11.531019289133571 18.529017281606528 40.64970230092877 29.290261128331124 5 12.424604308583788 38.20026320836447 36.87224787670348 12.502884606348259 6 28.788693338408056 39.49627721574478 19.226490048221184 12.488539397625981 7 24.57287510419515 32.44524866793984 25.989862238476036 16.992013989388973 8 22.889474710257893 36.86556411427384 23.345749808255178 16.899211367213084 9 23.901259948002558 17.341273480429344 23.223439614948017 35.53402695662008 10-11 22.214148618695592 28.143244548460196 30.211738094943264 19.430868737900944 12-13 23.28794511860122 25.845941202296647 30.113701582223833 20.7524120968783 14-15 20.891471787497725 27.265557471928236 27.30529011802289 24.537680622551147 16-17 20.204837811691114 30.90299752675454 28.32364374247196 20.568520919082385 18-19 20.205393391540227 29.91854719815727 29.843306688402816 20.03275272189969 20-21 21.760422518484514 29.360074111535596 28.587233840976445 20.29226952900344 22-23 20.542264221444427 29.469837120187712 29.109344522093362 20.8785541362745 24-25 20.94273833896487 29.15268936839319 28.612747426002706 21.29182486663923 26-27 21.0135032671885 29.048439964932594 28.715806765708894 21.22225000217001 28-29 20.69433418795459 28.994196361182684 29.26953781957751 21.041931631285216 30-31 21.464129248612327 29.50473484680848 28.406795623241926 20.62434028133727 32-33 20.385780498634592 29.283277623295938 29.197270129560366 21.1336717485091 34-35 20.540096386004482 28.611407077547103 29.133710192973794 21.714786343474625 36-37 21.22716097469595 29.310187599962862 28.58488946554055 20.877761959800644 38-39 20.955251112545696 28.958310787170387 28.83494473249868 21.251493367785233 40-41 21.153858376712282 29.06792874578546 28.436454309678382 21.341758567823874 42-43 20.81705229825245 29.16846288285711 29.027955207218405 20.986529611672033 44-45 21.42892014522487 28.551477751983185 28.780029229735167 21.239572873056783 46-47 21.122389932539694 29.215827509922125 28.97508944059597 20.68669311694221 48-49 20.925537116581346 28.827914148615196 29.143218682583704 21.103330052219757 50-51 21.273916220013255 28.905524366550033 28.720618250194175 21.099941163242534 52-53 20.568489748517784 29.0020511503504 28.92648838903254 21.502970712099273 54-55 21.103704207207734 28.948165698859995 28.533869996698453 21.41426009723382 56-57 21.320771772126744 28.894382856062677 28.490237230393728 21.294608141416848 58-59 20.934559687739398 28.3876457806579 29.026225491136458 21.651569040466242 60-61 21.47933949608339 28.917124118505726 28.41996648779786 21.183569897613026 62-63 20.74152789382277 29.070236658616817 28.966404557414872 21.22183089014554 64-65 20.754056286877717 28.474364512098777 29.001068084844196 21.770511116179307 66-67 21.04575638468072 28.76199806435047 28.684350866104406 21.507894684864407 68-69 21.50636073429215 28.55091164204473 28.61140903334795 21.331318590315167 70-71 21.535059845982826 28.717067490762332 28.28419926724234 21.4636733960125 72-73 21.148930834897193 28.87100316298622 28.830446438592094 21.149619563524492 74-75 21.522761616469687 28.27157091210154 28.44943623904217 21.7562312323866 76-77 21.31596464471988 28.833143437418762 28.6437780455357 21.20711387232566 78-79 21.33830557685093 28.554698233731624 28.841621172635573 21.26537501678187 80-81 21.412464705541577 28.498370346861723 28.523558185165875 21.56560676243083 82-83 20.77365700870159 28.83651726248939 28.929341055228214 21.460484673580805 84-85 21.092314203072483 28.34231377378469 28.48098153609787 22.084390487044953 86-87 21.339730472386 28.65343714017642 28.498507719823156 21.50832466761442 88-89 21.14180101104976 28.175348067903638 28.898030340815602 21.784820580231003 90-91 21.540120362480312 28.26876249132939 28.44376878245579 21.747348363734513 92-93 20.88112272023658 28.656240116408977 28.832775364969486 21.629861798384955 94-95 20.84777758549788 28.140070324822773 28.78265734543332 22.229494744246022 96-97 21.236851028900254 28.375418775435275 28.669784550921246 21.717945644743224 98-99 21.147652044611508 28.108740165264773 28.726336899514394 22.017270890609325 100 21.214713615820642 27.97140589047828 28.504780041259497 22.30910045244158 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 318.0 1 404.0 2 415.5 3 347.0 4 394.5 5 542.5 6 789.5 7 1175.0 8 1763.5 9 2542.5 10 3535.5 11 4631.0 12 5781.5 13 6898.5 14 7897.5 15 8841.0 16 9818.5 17 10755.5 18 11580.0 19 12586.5 20 13615.5 21 14910.5 22 16471.5 23 18433.5 24 21241.5 25 24885.5 26 30197.0 27 36939.0 28 45391.5 29 55965.5 30 67180.5 31 78414.0 32 89650.0 33 100267.5 34 109627.0 35 117648.5 36 124329.5 37 129183.0 38 132302.5 39 133951.5 40 133681.5 41 131061.0 42 126677.0 43 121156.5 44 114617.0 45 107994.0 46 101690.5 47 96246.5 48 91757.5 49 89476.5 50 88882.0 51 82520.0 52 71030.5 53 61405.5 54 54068.5 55 48556.5 56 43893.0 57 38992.0 58 34266.5 59 29551.0 60 25066.5 61 20873.5 62 16833.0 63 13422.0 64 10778.0 65 8542.0 66 6604.5 67 5057.5 68 3885.0 69 3003.5 70 2242.5 71 1667.0 72 1272.0 73 948.5 74 712.0 75 520.0 76 381.5 77 277.5 78 176.0 79 116.0 80 92.0 81 67.5 82 46.0 83 27.0 84 18.5 85 15.5 86 10.0 87 6.5 88 5.0 89 4.0 90 3.5 91 3.0 92 3.0 93 2.0 94 0.5 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.387911307552826 2 23.98368867443179 3 5.86295845911711 4 21.754809397860488 5 11.37267350140699 6 9.223135947661609 7 7.7025635520413225 8 8.538674771121341 9 4.8047089250800665 10-11 6.579517249421133 12-13 9.431639332024133 14-15 7.065423605211381 16-17 7.607843971972505 18-19 6.521059798116652 20-21 7.527900708090943 22-23 5.55361211522195 24-25 5.065700622579569 26-27 9.374893959108782 28-29 6.096711143686873 30-31 4.157188539007932 32-33 8.431693007995252 34-35 6.744077288462689 36-37 6.46611904836822 38-39 5.7918994939034585 40-41 7.783663625645268 42-43 6.186896029643943 44-45 6.166829236992986 46-47 5.695113080463982 48-49 3.8512817760206612 50-51 6.30714254425337 52-53 4.266113742467626 54-55 4.695536935546264 56-57 4.614760768664585 58-59 3.517627003825801 60-61 3.9725771314450493 62-63 3.0273710434793872 64-65 4.372216330204703 66-67 2.3864676090192907 68-69 4.825192169231082 70-71 2.360123195608195 72-73 1.4616676445564853 74-75 0.7772373162754799 76-77 1.1502390431610336 78-79 1.656860005046775 80-81 2.021733215616376 82-83 0.7824660962206563 84-85 1.1936579680745196 86-87 0.4441532368769962 88-89 0.7320754647136017 90-91 0.3372640185286985 92-93 0.3179838564889622 94-95 0.5308368454076504 96-97 0.5227083290916976 98-99 0.46189098595355366 100 0.4410067144321113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3241674.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.6206958610427 #Duplication Level Percentage of deduplicated Percentage of total 1 97.31119461502158 93.04964144157715 2 2.2728889934235696 4.346704543321333 3 0.24121765685801416 0.691962006082006 4 0.07491544449179113 0.28653867732177546 5 0.030655686871070818 0.1465659055355011 6 0.017171578942383785 0.09851749965021392 7 0.011372199904986065 0.07611923678699559 8 0.007217034663239538 0.05520783012417846 9 0.005601476178036263 0.048205534499359326 >10 0.025378468118982433 0.41302937568138426 >50 9.523803811411291E-4 0.06432847319925243 >100 0.0010776656676360576 0.25409604576632927 >500 1.9461789731325873E-4 0.1368418474341742 >1k 1.621815802875312E-4 0.3322415830203687 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3845 0.11861155686845747 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.0848259263578014E-5 0.0 2 0.0 0.0 0.0 3.0848259263578014E-5 0.0 3 0.0 0.0 0.0 3.0848259263578014E-5 0.0 4 0.0 0.0 0.0 3.0848259263578014E-5 0.0 5 0.0 0.0 0.0 3.0848259263578014E-5 0.0 6 0.0 0.0 0.0 3.0848259263578014E-5 0.0 7 0.0 0.0 0.0 3.0848259263578014E-5 0.0 8 0.0 0.0 0.0 3.0848259263578014E-5 0.0 9 0.0 0.0 0.0 9.254477779073404E-5 0.0 10-11 0.0 0.0 0.0 9.254477779073404E-5 0.0 12-13 1.5424129631789007E-5 0.0 0.0 9.254477779073404E-5 0.0 14-15 3.0848259263578014E-5 3.0848259263578014E-5 0.0 1.2339303705431205E-4 0.0 16-17 3.0848259263578014E-5 3.0848259263578014E-5 0.0 1.5424129631789007E-4 0.0 18-19 3.0848259263578014E-5 4.627238889536702E-5 0.0 1.6966542594967906E-4 0.0 20-21 3.0848259263578014E-5 9.254477779073404E-5 0.0 2.6221020374041313E-4 0.0 22-23 3.0848259263578014E-5 1.2339303705431205E-4 0.0 3.2390672226756916E-4 0.0 24-25 3.0848259263578014E-5 1.2339303705431205E-4 0.0 3.5475498153114714E-4 0.0 26-27 3.0848259263578014E-5 1.2339303705431205E-4 0.0 4.627238889536702E-4 1.5424129631789007E-5 28-29 3.0848259263578014E-5 1.2339303705431205E-4 0.0 8.483271297483955E-4 3.0848259263578014E-5 30-31 6.169651852715603E-5 1.2339303705431205E-4 0.0 0.002251922926241195 3.0848259263578014E-5 32-33 6.169651852715603E-5 1.2339303705431205E-4 0.0 0.005876593389711612 3.0848259263578014E-5 34-35 7.712064815894503E-5 1.2339303705431205E-4 0.0 0.012817451724016667 3.0848259263578014E-5 36-37 1.0796890742252305E-4 1.5424129631789007E-4 0.0 0.02284313598467952 3.0848259263578014E-5 38-39 1.2339303705431205E-4 1.5424129631789007E-4 0.0 0.03835981039425926 3.0848259263578014E-5 40-41 1.2339303705431205E-4 1.5424129631789007E-4 0.0 0.05804099980442204 3.0848259263578014E-5 42-43 1.2339303705431205E-4 1.5424129631789007E-4 0.0 0.0777376133442166 3.0848259263578014E-5 44-45 1.2339303705431205E-4 1.5424129631789007E-4 0.0 0.10277097573661015 3.0848259263578014E-5 46-47 1.2339303705431205E-4 1.5424129631789007E-4 0.0 0.13439044148177762 6.169651852715603E-5 48-49 1.2339303705431205E-4 1.6966542594967906E-4 0.0 0.16651890350479412 6.169651852715603E-5 50-51 1.2339303705431205E-4 2.159378148450461E-4 0.0 0.20017435436135775 6.169651852715603E-5 52-53 1.2339303705431205E-4 2.159378148450461E-4 0.0 0.23605087988489898 6.169651852715603E-5 54-55 1.2339303705431205E-4 2.159378148450461E-4 0.0 0.2771253370943531 6.169651852715603E-5 56-57 1.2339303705431205E-4 2.159378148450461E-4 0.0 0.32659052082350043 6.169651852715603E-5 58-59 1.2339303705431205E-4 2.159378148450461E-4 0.0 0.3827497768128443 6.169651852715603E-5 60-61 1.2339303705431205E-4 2.7763433337220215E-4 0.0 0.440590262932053 6.169651852715603E-5 62-63 1.2339303705431205E-4 2.7763433337220215E-4 0.0 0.5024718710147905 6.169651852715603E-5 64-65 1.2339303705431205E-4 2.7763433337220215E-4 0.0 0.5755205489509433 6.169651852715603E-5 66-67 1.2339303705431205E-4 2.7763433337220215E-4 0.0 0.6610781960184768 6.169651852715603E-5 68-69 1.2339303705431205E-4 2.7763433337220215E-4 0.0 0.756245075846615 6.169651852715603E-5 70-71 1.2339303705431205E-4 2.7763433337220215E-4 0.0 0.8708926313997027 6.169651852715603E-5 72-73 1.2339303705431205E-4 2.7763433337220215E-4 0.0 0.9881314407309311 6.169651852715603E-5 74-75 1.2339303705431205E-4 2.7763433337220215E-4 0.0 1.116506471656311 6.169651852715603E-5 76-77 1.2339303705431205E-4 2.7763433337220215E-4 0.0 1.2585935538243511 6.169651852715603E-5 78-79 1.2339303705431205E-4 2.9305846300399117E-4 0.0 1.4194518017542788 6.169651852715603E-5 80-81 1.2339303705431205E-4 3.2390672226756916E-4 0.0 1.6014102590204937 6.169651852715603E-5 82-83 1.2339303705431205E-4 3.393308518993582E-4 0.0 1.8033429641598755 6.169651852715603E-5 84-85 1.2339303705431205E-4 3.393308518993582E-4 0.0 2.0115532900593953 6.169651852715603E-5 86-87 1.2339303705431205E-4 3.393308518993582E-4 0.0 2.2367918550724104 6.169651852715603E-5 88 1.2339303705431205E-4 3.393308518993582E-4 0.0 2.4246423298579685 6.169651852715603E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTCG 135 0.007102326 18.470537 9 GTATACG 250 2.4116605E-4 16.92301 1 GTATATA 2595 0.0 16.303478 1 GTCTCGC 465 4.2611646E-8 15.597244 1 GTACATG 14740 0.0 14.925313 1 CTAATAC 1625 0.0 14.465605 3 TACATCG 335 1.3570845E-4 14.430096 2 CTGTCGC 660 0.0 14.356645 9 TACATGG 14880 0.0 14.334938 2 GTATAAG 1265 0.0 14.333436 1 TACGGGT 310 1.6438495E-4 14.108334 4 GTACTAA 1100 0.0 13.736209 1 TCTGTCG 585 2.3101165E-10 13.636067 8 GGACCGT 220 0.0072308 13.589683 6 GTATTAT 1700 0.0 13.509967 1 ACATGGG 14690 0.0 13.471261 3 TACACCG 295 2.463555E-4 13.451166 5 GGTATAC 590 5.3933036E-8 13.31714 1 GTATTAA 1775 0.0 13.279626 1 TAACCGT 265 0.0016223597 13.1623335 6 >>END_MODULE