FastQCFastQC Report
Wed 25 May 2016
SRR1295126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241674
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT100540.31014839863601334No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74960.23123855143978078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG1851.6449367E-417.7921265
AGCACCG4253.6379788E-1217.7024175
GAGTACT40650.015.65103812-13
ACTCGCG2751.2597859E-515.3667118
GTATATA30600.015.0776191
AGTACTT49050.014.50233812-13
CTATACT15400.014.35088354
CTGTCGC5753.092282E-1113.881799
GTATCAA148250.013.4859521
GTACATG175900.013.4813481
GTATTAA17800.013.378041
TACATGG177000.013.0499492
GTCTATA13000.012.9749831
ACATGGG169050.012.8505473
CTCGCGT3307.017248E-512.8053959
GTACGGA3507.2707E-512.7569171
AACGCAG159000.012.552496
CAACGCA158150.012.547145
GTACTTT51700.012.44173714-15
ATCAACG162050.012.361053
Produced by FastQC (version 0.11.5)