##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3241674 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.425329937556953 33.0 33.0 33.0 27.0 33.0 2 30.14064060729117 33.0 33.0 33.0 27.0 33.0 3 30.933538042381805 33.0 33.0 33.0 27.0 33.0 4 35.20756066155943 37.0 37.0 37.0 33.0 37.0 5 35.495485048774185 37.0 37.0 37.0 33.0 37.0 6 35.251067504011814 37.0 37.0 37.0 33.0 37.0 7 35.1551581065832 37.0 37.0 37.0 33.0 37.0 8 35.13980400249994 37.0 37.0 37.0 33.0 37.0 9 35.11394452372447 37.0 37.0 37.0 33.0 37.0 10-11 35.09056385682212 37.0 37.0 37.0 33.0 37.0 12-13 35.00940486304299 37.0 37.0 37.0 33.0 37.0 14-15 36.46842202516354 40.0 37.0 40.0 33.0 40.0 16-17 36.46261499459847 40.0 37.0 40.0 33.0 40.0 18-19 36.49330114625962 40.0 37.0 40.0 33.0 40.0 20-21 36.49606422484186 40.0 37.0 40.0 33.0 40.0 22-23 36.4517436053101 40.0 37.0 40.0 33.0 40.0 24-25 36.42512880690656 40.0 37.0 40.0 33.0 40.0 26-27 36.3968221357237 40.0 37.0 40.0 33.0 40.0 28-29 36.347811809577394 38.5 37.0 40.0 33.0 40.0 30-31 36.255194846859986 37.0 37.0 40.0 27.0 40.0 32-33 36.21112332702178 37.0 37.0 40.0 27.0 40.0 34-35 36.12828017252814 37.0 37.0 40.0 27.0 40.0 36-37 36.11810533693394 37.0 37.0 40.0 27.0 40.0 38-39 36.06063981140608 37.0 37.0 40.0 27.0 40.0 40-41 36.002709711093715 37.0 37.0 40.0 27.0 40.0 42-43 35.91644394223478 37.0 37.0 40.0 27.0 40.0 44-45 35.81910411102412 37.0 37.0 40.0 27.0 40.0 46-47 35.71499169873343 37.0 37.0 40.0 27.0 40.0 48-49 35.614386733521016 37.0 37.0 40.0 27.0 40.0 50-51 35.522288638524415 37.0 37.0 40.0 27.0 40.0 52-53 35.433149662797675 37.0 37.0 40.0 27.0 40.0 54-55 35.323234538698216 37.0 33.0 40.0 27.0 40.0 56-57 35.206048325649036 37.0 33.0 40.0 27.0 40.0 58-59 35.08534741618065 37.0 33.0 40.0 27.0 40.0 60-61 34.933098146204706 37.0 33.0 40.0 27.0 40.0 62-63 34.7598939621936 37.0 33.0 40.0 27.0 40.0 64-65 34.589622676431986 37.0 33.0 40.0 27.0 40.0 66-67 34.37053448310965 37.0 33.0 40.0 27.0 40.0 68-69 34.15734247182166 37.0 33.0 38.5 27.0 40.0 70-71 33.92552520703809 37.0 33.0 37.0 27.0 40.0 72-73 33.70357444949739 37.0 33.0 37.0 24.5 40.0 74-75 33.46082147680488 37.0 33.0 37.0 22.0 40.0 76-77 33.21670115502052 37.0 33.0 37.0 22.0 38.5 78-79 32.976567662263385 37.0 33.0 37.0 22.0 37.0 80-81 32.76007673812974 37.0 33.0 37.0 22.0 37.0 82-83 32.545603907117126 37.0 33.0 37.0 22.0 37.0 84-85 32.362696248913366 35.0 33.0 37.0 22.0 37.0 86-87 32.37653786284494 35.0 33.0 37.0 22.0 37.0 88-89 32.38035240434418 33.0 33.0 37.0 22.0 37.0 90-91 32.37823451710443 33.0 33.0 37.0 22.0 37.0 92-93 32.339852187480915 33.0 33.0 37.0 22.0 37.0 94-95 32.35951856972663 33.0 33.0 37.0 22.0 37.0 96-97 32.312281401522796 33.0 33.0 37.0 22.0 37.0 98-99 32.23643771705606 33.0 33.0 37.0 22.0 37.0 100 31.699364279073095 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48217795436984545 1101 2 4.016460744616968 1101 3 2.249806075987955 1101 4 1.0176492056310735 1101 5 0.41395583206655573 1101 6 0.3946944493381821 1101 7 0.5383330789135812 1101 8 0.4532566466067749 1101 9 0.5630864003585074 1101 10-11 0.5153939899583833 1101 12-13 0.5254370865870897 1101 14-15 0.8292621190143095 1101 16-17 0.8033544819520131 1101 18-19 0.9061363070394819 1101 20-21 0.8567142352341293 1101 22-23 0.7824461074451676 1101 24-25 0.7582069831738565 1101 26-27 0.90012394110515 1101 28-29 0.7722928488269432 1101 30-31 0.6696012871535117 1101 32-33 0.8875805610428102 1101 34-35 0.9768485487843677 1101 36-37 0.9362758533126296 1101 38-39 0.8731213468348216 1101 40-41 0.8964194859991395 1101 42-43 0.9509804187459423 1101 44-45 0.9282589591885113 1101 46-47 0.9924210808269223 1101 48-49 0.8964260286954584 1101 50-51 0.8500274535122259 1101 52-53 0.7954646497072204 1101 54-55 0.8090489365764952 1101 56-57 0.8709112339346134 1101 58-59 0.8941093771217581 1101 60-61 0.8625986732263655 1101 62-63 0.9235189733304736 1101 64-65 0.845614819209878 1101 66-67 0.8589580369629957 1101 68-69 0.7887386244817733 1101 70-71 0.8187857129839102 1101 72-73 0.7434869580962129 1101 74-75 0.710938707706724 1101 76-77 0.7301649119808005 1101 78-79 0.8195871481119283 1101 80-81 0.7844738780649791 1101 82-83 0.8569716474628635 1101 84-85 0.8014606967884035 1101 86-87 0.840769603136458 1101 88-89 0.8137750292606256 1101 90-91 0.7911848956338119 1101 92-93 0.8399289815819628 1101 94-95 0.8998240258231931 1101 96-97 0.760143283855065 1101 98-99 0.8042263669203393 1101 100 0.8891555365827521 1102 1 0.4391713195687359 1102 2 3.9705533076251918 1102 3 2.2512792649335793 1102 4 0.939586908679928 1102 5 0.46044412224711806 1102 6 0.4289985864158865 1102 7 0.42195326692173296 1102 8 0.44579809205506393 1102 9 0.4626884369870794 1102 10-11 0.5113046828782117 1102 12-13 0.5894380220192375 1102 14-15 0.8678151692756302 1102 16-17 0.8038319056458931 1102 18-19 0.7868133439159877 1102 20-21 0.7098369145194638 1102 22-23 0.759325490733751 1102 24-25 0.8245310396766357 1102 26-27 0.8304801505719439 1102 28-29 0.775043561120448 1102 30-31 0.7187661653081534 1102 32-33 0.8562332933826227 1102 34-35 0.8567054509288852 1102 36-37 0.7428919209857625 1102 38-39 0.7458730428342548 1102 40-41 0.7938831927926486 1102 42-43 0.8564624681911255 1102 44-45 0.8328250828942458 1102 46-47 0.8413108166541221 1102 48-49 0.7438405702462134 1102 50-51 0.6910173252789775 1102 52-53 0.8290037074992469 1102 54-55 0.7059451712069063 1102 56-57 0.6957454131286411 1102 58-59 0.7649329657626254 1102 60-61 0.7759524118338987 1102 62-63 0.8151493277096193 1102 64-65 0.7097554696866979 1102 66-67 0.7240484941550847 1102 68-69 0.6831753117394115 1102 70-71 0.7098995944089239 1102 72-73 0.7690279635384556 1102 74-75 0.7481569368493837 1102 76-77 0.6754901595042568 1102 78-79 0.6704509316038738 1102 80-81 0.6551561729189004 1102 82-83 0.7383589269421051 1102 84-85 0.7429704378105058 1102 86-87 0.7464069927995141 1102 88-89 0.6627786314181634 1102 90-91 0.6467672681341483 1102 92-93 0.7259813350819684 1102 94-95 0.7381021251750184 1102 96-97 0.7710990168698402 1102 98-99 0.8162825890316654 1102 100 0.8124677480703362 1103 1 0.4130620891146002 1103 2 3.9418197686210377 1103 3 2.221207548428346 1103 4 0.9939926632310616 1103 5 0.3762421561294502 1103 6 0.3753387502042642 1103 7 0.27535004757174875 1103 8 0.316449250607036 1103 9 0.3664418850179132 1103 10-11 0.4419206083503795 1103 12-13 0.4335624689464268 1103 14-15 0.634774382628656 1103 16-17 0.5559207147579883 1103 18-19 0.5898863335359934 1103 20-21 0.6447665696648173 1103 22-23 0.5833687250558128 1103 24-25 0.5408038337989112 1103 26-27 0.6210813762313876 1103 28-29 0.6560558144515554 1103 30-31 0.629727869804654 1103 32-33 0.7691991219497467 1103 34-35 0.7682131583259348 1103 36-37 0.5767792913817473 1103 38-39 0.48469159172732645 1103 40-41 0.5234352354499308 1103 42-43 0.5969703636155401 1103 44-45 0.7218875573407075 1103 46-47 0.7583968743531813 1103 48-49 0.6063335919470489 1103 50-51 0.6427989540300842 1103 52-53 0.6538824449533891 1103 54-55 0.6151747507553011 1103 56-57 0.6011196764433748 1103 58-59 0.6131358424228068 1103 60-61 0.6474588776498749 1103 62-63 0.7089885606385238 1103 64-65 0.5225489594158574 1103 66-67 0.5464635938695395 1103 68-69 0.6079929155912467 1103 70-71 0.6185129148731718 1103 72-73 0.5657120413688475 1103 74-75 0.6230963985831508 1103 76-77 0.49492745064362254 1103 78-79 0.5414932870289988 1103 80-81 0.5125494068763956 1103 82-83 0.5516757995981578 1103 84-85 0.5898283304647549 1103 86-87 0.5117284771702941 1103 88-89 0.5726859337218713 1103 90-91 0.5736657044338287 1103 92-93 0.4911035846642733 1103 94-95 0.5402852994838412 1103 96-97 0.48881693453510877 1103 98-99 0.48761465168067275 1103 100 0.5470251172372365 1104 1 0.38157694497373384 1104 2 3.5527030057844833 1104 3 2.095919927739242 1104 4 0.9110730319205445 1104 5 0.381542552075274 1104 6 0.3583946974688672 1104 7 0.27887882507035755 1104 8 0.2566339454164748 1104 9 0.3413603126217879 1104 10-11 0.3365877149124472 1104 12-13 0.3861317409152747 1104 14-15 0.40323163491841996 1104 16-17 0.4872269600240884 1104 18-19 0.4454505915761615 1104 20-21 0.5430278920043392 1104 22-23 0.4753309923491926 1104 24-25 0.5356223663836985 1104 26-27 0.5064696126263044 1104 28-29 0.4868991288426301 1104 30-31 0.39989055075471924 1104 32-33 0.598734041761638 1104 34-35 0.6048884417144009 1104 36-37 0.44561516295103587 1104 38-39 0.4398521491438814 1104 40-41 0.4494745411485539 1104 42-43 0.4548335419250549 1104 44-45 0.4728867348864725 1104 46-47 0.542079413827949 1104 48-49 0.37282019030359237 1104 50-51 0.3577097531611315 1104 52-53 0.4437446733567043 1104 54-55 0.40338793262925066 1104 56-57 0.43487529389486923 1104 58-59 0.5026466142826749 1104 60-61 0.49295737949474727 1104 62-63 0.5295263592210091 1104 64-65 0.44082188580103576 1104 66-67 0.367977325567729 1104 68-69 0.445330648588957 1104 70-71 0.5014717920280276 1104 72-73 0.4621263661476931 1104 74-75 0.36906560893594786 1104 76-77 0.4109866815799421 1104 78-79 0.40038568893802307 1104 80-81 0.428153585405866 1104 82-83 0.46460423839302933 1104 84-85 0.44253704808586036 1104 86-87 0.4257704892112031 1104 88-89 0.3484852962109173 1104 90-91 0.4079975668597555 1104 92-93 0.3982151512125256 1104 94-95 0.41329101347558606 1104 96-97 0.3933430184195075 1104 98-99 0.38255705752893476 1104 100 0.31280535508250296 1105 1 0.3515423703014058 1105 2 3.6743849598871208 1105 3 2.097008665849767 1105 4 0.9045677910760048 1105 5 0.223929176909202 1105 6 0.26807967432083046 1105 7 0.23514945897330364 1105 8 0.16611648789796618 1105 9 0.17823592833254054 1105 10-11 0.18601206933530534 1105 12-13 0.21330372739879522 1105 14-15 0.3444390302424978 1105 16-17 0.24133380926167547 1105 18-19 0.3568369131893334 1105 20-21 0.2712045985803613 1105 22-23 0.26684071717343016 1105 24-25 0.30762427611734466 1105 26-27 0.29189101797498296 1105 28-29 0.3085172758154968 1105 30-31 0.2637345008705836 1105 32-33 0.3207107673423337 1105 34-35 0.3688889860068656 1105 36-37 0.2212531275078149 1105 38-39 0.14849163183398417 1105 40-41 0.1566581065793784 1105 42-43 0.310848596930704 1105 44-45 0.3248922854902503 1105 46-47 0.3309925200854309 1105 48-49 0.19914858855642592 1105 50-51 0.20676912069244224 1105 52-53 0.1611574449263955 1105 54-55 0.13450029178462586 1105 56-57 0.10005895440647805 1105 58-59 0.12827523446277667 1105 60-61 0.14523555690686152 1105 62-63 0.19823956340442095 1105 64-65 0.12898884535263022 1105 66-67 0.14603105225850044 1105 68-69 0.06351309964470175 1105 70-71 0.016925453425919557 1105 72-73 0.1713808392091849 1105 74-75 0.10412222172854513 1105 76-77 0.06744900683574429 1105 78-79 0.11336774595421417 1105 80-81 0.12410078918946255 1105 82-83 0.1885875494377771 1105 84-85 0.08316335931176155 1105 86-87 0.1853111399110574 1105 88-89 0.1767816713107635 1105 90-91 0.10470342566043911 1105 92-93 0.15449095654491174 1105 94-95 0.20555934545103582 1105 96-97 0.10823148719065045 1105 98-99 0.1987871819650877 1105 100 0.16391764224759697 1106 1 0.44069187634936213 1106 2 3.8421469691954577 1106 3 2.172776016274554 1106 4 0.8716642328359399 1106 5 0.2337208066767218 1106 6 0.17730979379074796 1106 7 0.04972922367777244 1106 8 0.05446583120327375 1106 9 0.1523726276477646 1106 10-11 0.08778658185473631 1106 12-13 0.146545041852427 1106 14-15 0.10701051995859956 1106 16-17 0.12399969039793035 1106 18-19 0.15015813472451356 1106 20-21 0.15155648501447416 1106 22-23 0.09196028083325558 1106 24-25 0.14234310268080463 1106 26-27 0.15250370401942348 1106 28-29 0.14532704820005193 1106 30-31 0.08013549020971311 1106 32-33 0.18252495245876332 1106 34-35 0.34716166294168005 1106 36-37 0.22704149810009966 1106 38-39 0.1346579282911975 1106 40-41 0.0731418169874658 1106 42-43 0.13769271364869695 1106 44-45 0.14885082342170364 1106 46-47 0.09888562890437669 1106 48-49 0.01511626667691246 1106 50-51 0.09734771112019303 1106 52-53 0.09091913238160743 1106 54-55 0.06074140497105418 1106 56-57 0.06340499586418247 1106 58-59 0.05286125329408975 1106 60-61 0.001643963009911431 1106 62-63 0.05121754558322067 1106 64-65 0.063008470734772 1106 66-67 -0.07100769839933463 1106 68-69 -0.004227931770401483 1106 70-71 0.018047304369929407 1106 72-73 0.025550481740843622 1106 74-75 0.01298416647099998 1106 76-77 0.04811456831789229 1106 78-79 0.11367902103723537 1106 80-81 0.02293631137872154 1106 82-83 0.05228445592268116 1106 84-85 0.11777415332691277 1106 86-87 0.2139133011421066 1106 88-89 0.2078720806278227 1106 90-91 0.14384353753254686 1106 92-93 0.15967508950039644 1106 94-95 0.06249449269030549 1106 96-97 0.027986524316276018 1106 98-99 0.08575605250420892 1106 100 0.16360367526068842 1107 1 0.4793075085150953 1107 2 3.64874139073347 1107 3 2.143172390240732 1107 4 0.9595922314430751 1107 5 0.31562716285549186 1107 6 0.2913666239950885 1107 7 0.16967777796040906 1107 8 0.20310965516365798 1107 9 0.20345995359713243 1107 10-11 0.18945425005251337 1107 12-13 0.21159481861118934 1107 14-15 0.17228782439045176 1107 16-17 0.13567414553810409 1107 18-19 0.1991302378922697 1107 20-21 0.23002787019085957 1107 22-23 0.22016868904750453 1107 24-25 0.24845269193765063 1107 26-27 0.18347393854612193 1107 28-29 0.13157367529357344 1107 30-31 0.10386854166408455 1107 32-33 0.2722690107750836 1107 34-35 0.3926708524281679 1107 36-37 0.1654745077544817 1107 38-39 0.20183107574909087 1107 40-41 0.20028061609982473 1107 42-43 0.2645672414885709 1107 44-45 0.3513239425061272 1107 46-47 0.2269872077836652 1107 48-49 0.18082332112831523 1107 50-51 0.08979300292207171 1107 52-53 0.08404422950361123 1107 54-55 0.14126494453870464 1107 56-57 0.07182466008733712 1107 58-59 0.13163719125389406 1107 60-61 0.19988752344558947 1107 62-63 0.21024627054016065 1107 64-65 0.16187136451797102 1107 66-67 0.15776071930549307 1107 68-69 0.09591205062229591 1107 70-71 0.14893348279622387 1107 72-73 0.15544144467109078 1107 74-75 0.1476892036695574 1107 76-77 0.12876796775773158 1107 78-79 0.1396626910511003 1107 80-81 0.2785137558533961 1107 82-83 0.18084120710391716 1107 84-85 0.19863747859887582 1107 86-87 0.2742677039765553 1107 88-89 0.2559949778121009 1107 90-91 0.23898994781348648 1107 92-93 0.30581672307442886 1107 94-95 0.17444535911022285 1107 96-97 0.15484878154828863 1107 98-99 0.21486802188862697 1107 100 0.20419673683719353 1108 1 0.36304939629721744 1108 2 3.6436588786950743 1108 3 2.0895880888658596 1108 4 0.8910958259508632 1108 5 0.21930228720599132 1108 6 0.0670371665906373 1108 7 -0.06554910388443602 1108 8 0.026804119963443895 1108 9 -0.014079651522813208 1108 10-11 -0.0031975393937173635 1108 12-13 -0.03546146968442088 1108 14-15 -0.12803367765506124 1108 16-17 -0.1357352975428654 1108 18-19 -0.11108047203830296 1108 20-21 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0.23054998829132245 2210 38-39 0.14448118254886566 2210 40-41 0.16012150991263496 2210 42-43 0.21441747950481727 2210 44-45 0.2524950122558849 2210 46-47 0.3222090829118045 2210 48-49 0.16430275083368429 2210 50-51 0.10807275619534096 2210 52-53 0.16810385098882108 2210 54-55 0.18335206929500458 2210 56-57 0.08888062714404299 2210 58-59 0.22830123607624841 2210 60-61 0.21458165610594193 2210 62-63 0.17704483709959362 2210 64-65 -0.26652681286532953 2210 66-67 0.0600827290050816 2210 68-69 0.19109835689099697 2210 70-71 0.12528019126328616 2210 72-73 0.062143083758691375 2210 74-75 -0.5751327706164844 2210 76-77 -0.08068617295453606 2210 78-79 0.12564126026965283 2210 80-81 0.23444720077043257 2210 82-83 0.17182178308956253 2210 84-85 0.21484709318776396 2210 86-87 0.21048217705418182 2210 88-89 0.2725424189044787 2210 90-91 0.24705833197456428 2210 92-93 0.25094484552325724 2210 94-95 0.2782334271091855 2210 96-97 0.19072797541193154 2210 98-99 0.16738607968600405 2210 100 0.05193809872999111 2211 1 0.3945134049928001 2211 2 3.7867925426940694 2211 3 2.1533630652822886 2211 4 0.8507703284453214 2211 5 0.34308174858693263 2211 6 0.06777910135718201 2211 7 0.04851072404188983 2211 8 0.03802671218183917 2211 9 -0.0666930542793267 2211 10-11 0.039112501327302596 2211 12-13 0.027575590750871015 2211 14-15 -0.1620976395719822 2211 16-17 -0.2689886436505873 2211 18-19 -0.12013204939818678 2211 20-21 -0.14497264642046304 2211 22-23 -0.19578474402074164 2211 24-25 -0.12094052532758326 2211 26-27 -0.21095033739210578 2211 28-29 -0.20480435855811407 2211 30-31 -0.16550417646368487 2211 32-33 -0.06028661586618256 2211 34-35 0.13039027792330415 2211 36-37 -0.07947509991021207 2211 38-39 -0.15849561272271018 2211 40-41 -0.24826634221667376 2211 42-43 -0.10513087978200986 2211 44-45 -0.020463664938695558 2211 46-47 -0.06913461164703705 2211 48-49 -0.10019701930615099 2211 50-51 -0.16671020378775836 2211 52-53 -0.11856773670256615 2211 54-55 -0.2241155459031603 2211 56-57 -0.15217367187801045 2211 58-59 -0.07933261632697963 2211 60-61 -0.10596033079892919 2211 62-63 -0.03268782969235673 2211 64-65 -0.24406156527991385 2211 66-67 -0.2502781249068491 2211 68-69 -0.17908997449410435 2211 70-71 -0.2493314883933948 2211 72-73 -0.11400876515576641 2211 74-75 -0.2025090097644835 2211 76-77 -0.29001711531750374 2211 78-79 -0.3337171538332484 2211 80-81 -0.2167218478420736 2211 82-83 -0.4843173677762067 2211 84-85 -0.25119956152405365 2211 86-87 -0.20571309048074937 2211 88-89 -0.0689216204552281 2211 90-91 -0.05909602788346291 2211 92-93 -0.11835961248984006 2211 94-95 -0.07348075637762008 2211 96-97 -0.23169448527030312 2211 98-99 -0.19027128958833117 2211 100 -0.3515933602609387 2212 1 0.4712102635490538 2212 2 4.069990444162581 2212 3 2.268361912822222 2212 4 1.0781856012621063 2212 5 0.6215963030606915 2212 6 0.37769213834445026 2212 7 0.27775483274864854 2212 8 0.28796908917845343 2212 9 0.09995936689824703 2212 10-11 0.10386715269316937 2212 12-13 0.1946223982940296 2212 14-15 0.3769228206541797 2212 16-17 0.3971750860236014 2212 18-19 0.3026406612591259 2212 20-21 0.3143717961150827 2212 22-23 0.2823187996016472 2212 24-25 0.2194715339917721 2212 26-27 0.14706209493113676 2212 28-29 0.08728899612066243 2212 30-31 0.1533386453625809 2212 32-33 0.4868350403257722 2212 34-35 0.2730305026829356 2212 36-37 0.305103175206078 2212 38-39 0.3060045572259753 2212 40-41 0.40049573902648916 2212 42-43 0.38222867037963226 2212 44-45 0.5694493036702326 2212 46-47 0.48611680271634583 2212 48-49 0.3200087966711891 2212 50-51 0.2958081337180758 2212 52-53 0.08368815045948708 2212 54-55 0.1795720556493663 2212 56-57 0.347524038843396 2212 58-59 0.20431366191078126 2212 60-61 0.3188671492053885 2212 62-63 0.3842042006117907 2212 64-65 0.35761888959564203 2212 66-67 0.39823957497089424 2212 68-69 0.43422569728185323 2212 70-71 0.30326955272393263 2212 72-73 0.3999042342234205 2212 74-75 0.4290172405407304 2212 76-77 0.5235917713604934 2212 78-79 0.5405657638594548 2212 80-81 0.4277728546878947 2212 82-83 0.5017508287990111 2212 84-85 0.48935703252765705 2212 86-87 0.5233197576227369 2212 88-89 0.6516754051436351 2212 90-91 0.4670318373473705 2212 92-93 0.5844900266576758 2212 94-95 0.4218677352957485 2212 96-97 0.33840350660008767 2212 98-99 0.3840435807364102 2212 100 0.27883078778046055 2213 1 -0.46966463135952097 2213 2 -25.905347195297196 2213 3 -14.810310990136909 2213 4 -6.162060759756205 2213 5 -2.2018951454285514 2213 6 -1.3791431620529124 2213 7 -1.2515313242485533 2213 8 -1.0354216254704198 2213 9 -1.09306432796469 2213 10-11 -1.0572987294568392 2213 12-13 -0.9817222626539746 2213 14-15 -1.2017891308161026 2213 16-17 -1.2571062606497634 2213 18-19 -1.2251365254461817 2213 20-21 -1.3269485008256439 2213 22-23 -1.2460162714847556 2213 24-25 -1.1837672740258256 2213 26-27 -1.2423741396424361 2213 28-29 -1.4008738721633875 2213 30-31 -1.420625912624125 2213 32-33 -1.1885077150548895 2213 34-35 -1.198003877663595 2213 36-37 -1.3435455652134536 2213 38-39 -1.4334738497396486 2213 40-41 -1.2727488096881672 2213 42-43 -1.3426846419256009 2213 44-45 -1.2252065736852984 2213 46-47 -1.2916083193074215 2213 48-49 -1.3847989465693473 2213 50-51 -1.3823541505360666 2213 52-53 -1.4932311913886096 2213 54-55 -1.290484414156964 2213 56-57 -1.2305050730101001 2213 58-59 -1.1623919249917378 2213 60-61 -1.380410532567133 2213 62-63 -1.204073051513916 2213 64-65 -1.1308315794618622 2213 66-67 -1.2566310080765675 2213 68-69 -1.3429390503108394 2213 70-71 -1.272481616128168 2213 72-73 -1.2066934474947715 2213 74-75 -1.2634031831209072 2213 76-77 -1.2710358099724672 2213 78-79 -1.2301110453090054 2213 80-81 -1.3097622946313443 2213 82-83 -1.2759261899863859 2213 84-85 -1.2076746241569296 2213 86-87 -1.1573358589566496 2213 88-89 -1.2173086906807349 2213 90-91 -1.122735030590789 2213 92-93 -1.175567172054194 2213 94-95 -1.3171641604336308 2213 96-97 -1.4392147696424402 2213 98-99 -1.2587458456964242 2213 100 -1.055969562275294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 12.0 4 38.0 5 46.0 6 41.0 7 52.0 8 48.0 9 99.0 10 412.0 11 1983.0 12 5786.0 13 10436.0 14 13759.0 15 16124.0 16 17325.0 17 18104.0 18 18636.0 19 19963.0 20 21708.0 21 24911.0 22 27191.0 23 28910.0 24 29654.0 25 31384.0 26 34100.0 27 39181.0 28 46038.0 29 55359.0 30 67277.0 31 83474.0 32 104485.0 33 140769.0 34 194033.0 35 276278.0 36 420882.0 37 720707.0 38 766089.0 39 6376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.980255502774796 21.497565863900164 10.498817013271374 30.023361620053667 2 12.397493440458154 23.46295033543167 36.919302921252005 27.220253302858165 3 14.763946035289175 28.535133390957885 29.196119042198564 27.504801531554374 4 10.087074557107488 19.584880339968688 38.69537787581271 31.632667227111117 5 11.223566009507925 39.10333457131222 35.75872141705531 13.914378002124545 6 28.642228401389048 40.713672162791724 18.27471612716244 12.369383308656792 7 24.65079721674643 33.81110585818837 24.52297838250851 17.0151185425567 8 23.59403537077717 37.564461584136296 21.68881172187037 17.152691323216168 9 24.329147784996465 16.972702999300076 22.76543071193412 35.93271850376934 10-11 22.312889102311477 28.37431345324569 29.747638421126858 19.565159023315974 12-13 23.277921760496834 26.00021355665022 30.047528698989435 20.67433598386351 14-15 21.04087172605858 27.197893764867526 27.194190385781 24.5670441232929 16-17 20.105854719851283 30.84907656005951 28.207831113844513 20.837237606244695 18-19 20.09142853616372 29.795952057874914 30.195284626475242 19.917334779486126 20-21 21.421692940317246 29.11276405427663 29.20292272057015 20.26262028483597 22-23 20.397059810441238 29.26732074493647 29.447376114546746 20.88824333007554 24-25 20.705095501801953 28.902062887054548 29.23497819929224 21.15786341185126 26-27 20.69133872892005 28.87190628672341 29.299652515000968 21.137102469355575 28-29 20.261838605983407 28.885151109329126 29.82545481360306 21.027555471084412 30-31 21.16409185339964 29.271536992119895 28.994596332491707 20.56977482198876 32-33 19.971208897755492 29.222490623148595 29.82043119403176 20.985869285064148 34-35 20.18452549430328 28.707928210435878 29.94589792098397 21.16164837427687 36-37 20.9763937078269 29.19287711345587 29.121722903634712 20.709006275082515 38-39 20.65444675682696 29.125762073320693 29.410400044917672 20.809391124934677 40-41 20.833967690878378 28.994790478451353 29.14137527481757 21.029866555852696 42-43 20.537094975669117 29.10819565217056 29.410945357434226 20.943764014726096 44-45 21.069007617809184 28.4902669896735 29.27203916187902 21.168686230638293 46-47 20.91366979723309 29.20508398227795 29.19732441471572 20.68392180577324 48-49 20.7740192042245 29.050867482396487 29.30022711273391 20.874886200645097 50-51 21.054611960342516 28.95139698287798 28.802291883452728 21.19169917332677 52-53 20.446079218665556 29.263445963969858 28.88554713637575 21.404927680988838 54-55 20.965062497493005 29.071547364491057 28.557296825293975 21.406093312721968 56-57 21.114544712389442 28.827065896862585 28.674420448658832 21.38396894208914 58-59 20.556743893736343 28.516275193823564 29.396205935972898 21.5307749764672 60-61 21.24497423252921 28.93415164798278 28.54441556710649 21.276458552381516 62-63 20.39457293294349 29.21586319809683 29.01084176186382 21.378722107095854 64-65 20.4326204755385 28.571494687313187 29.210436392386757 21.785448444761556 66-67 20.863334546417757 28.869167224999188 28.79982240320612 21.467675825376933 68-69 21.04255932342506 28.766083249968986 28.82680910912573 21.36454831748022 70-71 21.106953547311498 28.723482545354738 28.562921007333497 21.60664290000026 72-73 20.7717134565808 29.016740694343092 28.966615806387413 21.244930042688694 74-75 21.255432714106906 28.34673779508985 28.674208710948523 21.72362077985472 76-77 20.946756169243237 28.923588715369185 28.855216834662695 21.274438280724883 78-79 20.962281797963644 28.88125770636395 28.996938974004806 21.1595215216676 80-81 21.10496446658066 28.66079680196912 28.66715238544948 21.567086346000742 82-83 20.430684405097924 29.054676699783027 28.846464134776372 21.66817476034268 84-85 20.715121020705883 28.530719916898196 28.647153764717554 22.107005297678374 86-87 20.975002595331983 28.79453346728794 28.531622095599847 21.698841841780233 88-89 20.58007762277663 28.484637486578134 29.039050633428822 21.896234257216417 90-91 21.0009383179721 28.510659696304586 28.6591740056151 21.829227980108215 92-93 20.24473484744485 29.001798214372204 28.870518384883276 21.882948553299673 94-95 20.28909883160452 28.36688389310917 29.09537654725639 22.248640728029923 96-97 20.711427370977265 28.663117226537942 28.749986889344214 21.87546851314058 98-99 20.54027724457032 28.47973586295006 28.816570447677808 22.163416444801808 100 20.654692788497172 28.368054697729573 28.69049089606443 22.286761617708823 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10.0 1 10.5 2 18.5 3 20.5 4 18.0 5 26.0 6 52.5 7 96.0 8 166.0 9 198.0 10 181.0 11 187.0 12 217.5 13 262.5 14 317.0 15 398.5 16 509.0 17 665.5 18 874.5 19 1160.0 20 1593.0 21 2245.0 22 3308.5 23 4938.5 24 7461.0 25 11306.0 26 16689.0 27 24242.5 28 34113.0 29 45826.0 30 59018.5 31 72702.5 32 85821.0 33 98395.0 34 110221.0 35 120552.0 36 129427.5 37 136642.0 38 141326.0 39 144241.0 40 145634.5 41 143925.5 42 139717.5 43 133428.5 44 125851.0 45 118703.5 46 112167.0 47 106605.0 48 100990.0 49 98187.0 50 108480.0 51 102644.5 52 78198.0 53 65659.5 54 59027.5 55 53886.5 56 48996.5 57 43758.0 58 38391.5 59 33055.5 60 27828.0 61 22902.5 62 18525.5 63 14583.0 64 11250.5 65 8803.0 66 6735.0 67 5097.0 68 3841.0 69 2748.5 70 1950.5 71 1417.5 72 1039.5 73 729.5 74 504.5 75 352.0 76 229.0 77 150.0 78 86.0 79 52.5 80 32.0 81 19.0 82 15.0 83 9.0 84 6.0 85 3.0 86 2.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.6612324373147948 2 5.132625921051901 3 0.0 4 0.006138803593452025 5 0.16090452031882294 6 0.054138695007579424 7 0.035475498153114716 8 0.04232381170962904 9 0.040658005709395824 10-11 0.042046177376256835 12-13 0.040596309190868665 14-15 0.04287908037637344 16-17 0.0339485093195676 18-19 0.0275012231334798 20-21 0.022241594929039748 22-23 0.02241126035498943 24-25 0.0188020140211508 26-27 0.01181488329795038 28-29 0.018308441872933554 30-31 0.022426684484621217 32-33 0.0014652923150199559 34-35 0.003593822204206839 36-37 0.003794335889420096 38-39 0.005799472741552667 40-41 0.0063393172786652815 42-43 0.006709496389828219 44-45 0.007912578501107761 46-47 0.015856005261479098 48-49 0.023830280281114016 50-51 0.020714606095492638 52-53 0.026514078837045305 54-55 0.02295110489210204 56-57 0.015516674409579741 58-59 0.013974261446400842 60-61 0.01166064200163249 62-63 0.016951118465336118 64-65 0.018863710539677957 66-67 0.01926473791010447 68-69 0.02714646815194865 70-71 0.026544927096308884 72-73 0.023722311373691494 74-75 0.02392282505890475 76-77 0.017753173206189146 78-79 0.013588658205606117 80-81 0.013249327353706758 82-83 0.008483271297483955 84-85 0.01075061835335694 86-87 0.0085603919456429 88-89 0.007048827241727577 90-91 0.007264765056572624 92-93 0.0038560324079472516 94-95 0.0015115647039153227 96-97 0.0011105373334888086 98-99 7.249340926940834E-4 100 0.10840078305221314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3241674.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.22920021571586 #Duplication Level Percentage of deduplicated Percentage of total 1 90.59733021562401 78.12135326172368 2 7.627342870805473 13.153993510411963 3 1.1495202943382632 2.973666468375682 4 0.3011354917449436 1.0386669043893115 5 0.10927967680849376 0.4711549565514166 6 0.05591031426016063 0.28926610094777905 7 0.03274604864180718 0.19765659092255733 8 0.017775043188229745 0.12261822063366873 9 0.016187651388473907 0.12562634093390349 >10 0.08554607786779476 1.264288761647289 >50 0.004269636980349429 0.24600380377340098 >100 0.0017736488799643588 0.2922335893993208 >500 6.811381818625463E-4 0.42693435919728867 >1k 4.3019253447434415E-4 0.7340204426152592 >5k 3.5849377872862E-5 0.23172108813830206 >10k+ 3.5849377872862E-5 0.3107956003391794 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10054 0.31014839863601334 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7496 0.23123855143978078 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.0848259263578014E-5 0.0 4 0.0 0.0 0.0 3.0848259263578014E-5 0.0 5 0.0 0.0 0.0 3.0848259263578014E-5 0.0 6 0.0 0.0 0.0 3.0848259263578014E-5 0.0 7 3.0848259263578014E-5 3.0848259263578014E-5 0.0 3.0848259263578014E-5 0.0 8 6.169651852715603E-5 3.0848259263578014E-5 0.0 3.0848259263578014E-5 0.0 9 6.169651852715603E-5 3.0848259263578014E-5 0.0 6.169651852715603E-5 0.0 10-11 7.712064815894503E-5 3.0848259263578014E-5 0.0 9.254477779073404E-5 0.0 12-13 9.254477779073404E-5 3.0848259263578014E-5 0.0 1.2339303705431205E-4 0.0 14-15 9.254477779073404E-5 3.0848259263578014E-5 0.0 1.2339303705431205E-4 0.0 16-17 9.254477779073404E-5 3.0848259263578014E-5 0.0 1.5424129631789007E-4 0.0 18-19 1.0796890742252305E-4 3.0848259263578014E-5 0.0 1.5424129631789007E-4 0.0 20-21 1.2339303705431205E-4 3.0848259263578014E-5 0.0 2.005136852132571E-4 0.0 22-23 1.2339303705431205E-4 3.0848259263578014E-5 0.0 2.7763433337220215E-4 0.0 24-25 1.2339303705431205E-4 6.169651852715603E-5 0.0 3.393308518993581E-4 0.0 26-27 1.2339303705431205E-4 9.254477779073404E-5 0.0 4.781480185854592E-4 0.0 28-29 1.2339303705431205E-4 9.254477779073404E-5 0.0 9.408719075391295E-4 0.0 30-31 1.5424129631789007E-4 9.254477779073404E-5 0.0 0.0029305846300399117 0.0 32-33 1.5424129631789007E-4 9.254477779073404E-5 0.0 0.008097668056689229 0.0 34-35 1.6966542594967906E-4 9.254477779073404E-5 0.0 0.01759893190987126 3.0848259263578014E-5 36-37 2.005136852132571E-4 1.0796890742252305E-4 0.0 0.03219015854154366 3.0848259263578014E-5 38-39 2.313619444768351E-4 1.2339303705431205E-4 0.0 0.054323784563160886 3.0848259263578014E-5 40-41 2.467860741086241E-4 1.2339303705431205E-4 0.0 0.08196382486332679 3.0848259263578014E-5 42-43 2.467860741086241E-4 1.2339303705431205E-4 0.0 0.10895605171895756 3.0848259263578014E-5 44-45 2.6221020374041313E-4 1.2339303705431205E-4 0.0 0.14527987700182066 3.0848259263578014E-5 46-47 2.7763433337220215E-4 1.2339303705431205E-4 0.0 0.18967052208210944 3.0848259263578014E-5 48-49 2.7763433337220215E-4 1.2339303705431205E-4 0.0 0.23453931518098364 3.0848259263578014E-5 50-51 2.7763433337220215E-4 1.2339303705431205E-4 0.0 0.28082712820598243 3.0848259263578014E-5 52-53 2.7763433337220215E-4 1.5424129631789007E-4 0.0 0.33194269380573127 3.0848259263578014E-5 54-55 2.7763433337220215E-4 1.5424129631789007E-4 0.0 0.3887806114988738 3.0848259263578014E-5 56-57 3.0848259263578014E-4 1.5424129631789007E-4 0.0 0.45834343613824213 3.0848259263578014E-5 58-59 3.0848259263578014E-4 1.5424129631789007E-4 0.0 0.5370373455196297 3.0848259263578014E-5 60-61 3.0848259263578014E-4 1.5424129631789007E-4 0.0 0.6185384464940028 3.0848259263578014E-5 62-63 3.0848259263578014E-4 1.5424129631789007E-4 0.0 0.703031828616943 3.0848259263578014E-5 64-65 3.0848259263578014E-4 1.5424129631789007E-4 0.0 0.8006819933157991 3.0848259263578014E-5 66-67 3.0848259263578014E-4 1.8508955558146808E-4 0.0 0.9126920227018509 3.0848259263578014E-5 68-69 3.0848259263578014E-4 1.8508955558146808E-4 0.0 1.036686600811803 3.0848259263578014E-5 70-71 3.0848259263578014E-4 1.8508955558146808E-4 0.0 1.1848199417955043 3.0848259263578014E-5 72-73 3.0848259263578014E-4 1.8508955558146808E-4 0.0 1.3336782168719001 3.0848259263578014E-5 74-75 3.0848259263578014E-4 1.8508955558146808E-4 0.0 1.493209989653494 3.0848259263578014E-5 76-77 3.0848259263578014E-4 1.8508955558146808E-4 0.0 1.6711581732154435 3.0848259263578014E-5 78-79 3.0848259263578014E-4 2.159378148450461E-4 0.0 1.8714713447434876 3.0848259263578014E-5 80-81 3.2390672226756916E-4 2.467860741086241E-4 0.0 2.094303745533943 3.0848259263578014E-5 82-83 3.393308518993582E-4 2.467860741086241E-4 0.0 2.3365551255308215 3.0848259263578014E-5 84-85 3.393308518993582E-4 2.467860741086241E-4 0.0 2.590405451010805 3.0848259263578014E-5 86-87 3.393308518993582E-4 2.467860741086241E-4 0.0 2.863674755697211 3.0848259263578014E-5 88 3.393308518993582E-4 2.467860741086241E-4 0.0 3.088897896580594 3.0848259263578014E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATATCG 185 1.6449367E-4 17.792126 5 AGCACCG 425 3.6379788E-12 17.702417 5 GAGTACT 4065 0.0 15.651038 12-13 ACTCGCG 275 1.2597859E-5 15.366711 8 GTATATA 3060 0.0 15.077619 1 AGTACTT 4905 0.0 14.502338 12-13 CTATACT 1540 0.0 14.3508835 4 CTGTCGC 575 3.092282E-11 13.88179 9 GTATCAA 14825 0.0 13.485952 1 GTACATG 17590 0.0 13.481348 1 GTATTAA 1780 0.0 13.37804 1 TACATGG 17700 0.0 13.049949 2 GTCTATA 1300 0.0 12.974983 1 ACATGGG 16905 0.0 12.850547 3 CTCGCGT 330 7.017248E-5 12.805395 9 GTACGGA 350 7.2707E-5 12.756917 1 AACGCAG 15900 0.0 12.55249 6 CAACGCA 15815 0.0 12.54714 5 GTACTTT 5170 0.0 12.441737 14-15 ATCAACG 16205 0.0 12.36105 3 >>END_MODULE