FastQCFastQC Report
Wed 25 May 2016
SRR1295125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106844
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68100.6152628554701475No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51860.4685393786296894No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23540.212676763843866No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22990.20770768057648595No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20140.1819587945546075No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16010.14464549656500825No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15160.13696600424269365No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14350.1296478997943703No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12820.11582481361420399No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11670.10543491223695481No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA3750.025.8978251
GTATAGG2352.397628E-723.2460521
GTATATA1703.4366982E-421.4228321
TGTACTA3551.8189894E-1221.051035
GCTCTAG3401.9401341E-819.6375961
CGTGTCG1300.005720686619.1741629
TAAATAG3506.9303496E-1018.5048565
TCTATAG1900.001421277617.3481713
TACATCG3858.078132E-817.3377692
GTACTAT3209.34142E-817.119476
GTTCTAA3306.2045856E-616.5540081
TAATACA4654.620233E-1016.540814
ATGTACC8350.016.4447194
TACTATC2204.0774996E-415.7945237
CATGGGG29750.015.6968934
TCTACAC4203.9332008E-815.6959663
ACTGATC2951.3908648E-515.2044458
ATAGACG2908.93081E-515.1547253
CTATGCG2356.514202E-414.8497759
GTTTAAC3301.1477456E-414.7146751
Produced by FastQC (version 0.11.5)