##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295125_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1106844 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.93644542501021 27.0 22.0 33.0 6.0 33.0 2 21.960568065599126 27.0 6.0 33.0 2.0 33.0 3 24.308515924556666 27.0 15.0 33.0 15.0 33.0 4 24.852946756724524 33.0 15.0 37.0 2.0 37.0 5 26.537261800217557 33.0 15.0 37.0 2.0 37.0 6 27.192551073141292 33.0 15.0 37.0 2.0 37.0 7 26.824467585314643 33.0 15.0 37.0 6.0 37.0 8 27.665554495484457 33.0 15.0 37.0 6.0 37.0 9 28.583967569052188 33.0 22.0 37.0 6.0 37.0 10-11 28.180862434091885 33.0 18.5 37.0 6.0 37.0 12-13 27.045391220442987 33.0 15.0 37.0 4.0 37.0 14-15 28.66037716245469 33.0 18.5 37.0 6.0 40.0 16-17 29.020506051439952 33.0 22.0 37.0 6.0 40.0 18-19 29.12725777074276 33.0 22.0 37.0 6.0 40.0 20-21 28.585542316713106 33.0 18.5 37.0 6.0 40.0 22-23 29.376937942474278 33.0 22.0 37.0 6.0 40.0 24-25 29.51596159892451 33.0 24.5 37.0 6.0 40.0 26-27 28.062669174698513 33.0 18.5 37.0 4.0 40.0 28-29 28.76297834202471 33.0 22.0 37.0 6.0 40.0 30-31 29.43452013111152 33.0 22.0 37.0 6.0 40.0 32-33 28.136855780941126 33.0 22.0 37.0 6.0 40.0 34-35 27.840902150619236 33.0 18.5 37.0 6.0 40.0 36-37 28.08267876954657 33.0 22.0 37.0 6.0 40.0 38-39 28.179405137490015 33.0 22.0 37.0 6.0 40.0 40-41 27.221832525631434 33.0 15.0 37.0 6.0 40.0 42-43 27.807520300963823 33.0 22.0 37.0 6.0 40.0 44-45 27.547274503001326 33.0 22.0 37.0 6.0 40.0 46-47 27.12492501201615 33.0 15.0 37.0 6.0 40.0 48-49 26.932556891486062 33.0 15.0 37.0 6.0 40.0 50-51 26.83228395329423 33.0 15.0 37.0 6.0 40.0 52-53 27.081029485636638 33.0 18.5 37.0 6.0 40.0 54-55 26.97253406984182 33.0 22.0 37.0 6.0 40.0 56-57 26.85638310367134 33.0 18.5 37.0 6.0 40.0 58-59 26.713039055187544 33.0 18.5 37.0 6.0 38.5 60-61 26.660231703835407 30.0 18.5 37.0 6.0 37.0 62-63 26.468559254962756 27.0 22.0 37.0 6.0 37.0 64-65 26.007438265916427 27.0 15.0 37.0 6.0 37.0 66-67 26.170123793416238 27.0 18.5 35.0 6.0 37.0 68-69 25.723952517247238 27.0 18.5 33.0 6.0 37.0 70-71 25.774815601837297 27.0 18.5 33.0 6.0 37.0 72-73 25.730444850403487 27.0 18.5 33.0 6.0 37.0 74-75 25.783626689940046 27.0 22.0 33.0 6.0 37.0 76-77 25.51028871277253 27.0 22.0 33.0 6.0 37.0 78-79 25.099272797250563 27.0 15.0 33.0 6.0 37.0 80-81 24.980948986487707 27.0 15.0 33.0 6.0 37.0 82-83 25.146592473736135 27.0 15.0 33.0 6.0 37.0 84-85 25.02792489275815 27.0 18.5 33.0 6.0 37.0 86-87 24.759398795132828 27.0 15.0 33.0 6.0 37.0 88-89 24.69519327023501 27.0 15.0 33.0 6.0 37.0 90-91 24.863990318418857 27.0 15.0 33.0 6.0 37.0 92-93 24.783975880973287 27.0 15.0 33.0 6.0 37.0 94-95 24.669927740494593 27.0 15.0 33.0 6.0 37.0 96-97 24.78261841777161 27.0 15.0 33.0 6.0 37.0 98-99 24.820373964172006 27.0 15.0 33.0 6.0 37.0 100 24.451853197017826 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.2780460573889485 1101 2 11.01157544591716 1101 3 8.300565089326941 1101 4 12.6216459525414 1101 5 10.655928004109878 1101 6 10.052349218523606 1101 7 9.520424877571589 1101 8 9.061697002467874 1101 9 7.301588722529374 1101 10-11 7.729479893247266 1101 12-13 8.901988333443075 1101 14-15 7.8796095350264785 1101 16-17 8.157503541956594 1101 18-19 8.048573489351345 1101 20-21 7.163548909363833 1101 22-23 7.340385408542751 1101 24-25 7.352772740530948 1101 26-27 8.405007015044877 1101 28-29 8.255409325006536 1101 30-31 5.210569508087087 1101 32-33 6.752832546853934 1101 34-35 8.650522293287843 1101 36-37 8.226296177776632 1101 38-39 8.02631488655506 1101 40-41 8.946752697586138 1101 42-43 8.429140461421838 1101 44-45 8.315268745888577 1101 46-47 7.130623440259292 1101 48-49 4.942961640556131 1101 50-51 6.428655992999602 1101 52-53 5.557048476716567 1101 54-55 3.765384903791304 1101 56-57 5.803172264533536 1101 58-59 5.861433664146784 1101 60-61 6.415591977291108 1101 62-63 6.067842634917309 1101 64-65 6.448969992616803 1101 66-67 5.65563788646892 1101 68-69 6.576778113711921 1101 70-71 5.948790340142548 1101 72-73 5.188245557181197 1101 74-75 4.471012011401459 1101 76-77 4.696904580141911 1101 78-79 4.839719829812342 1101 80-81 4.621644817215262 1101 82-83 4.107203096208988 1101 84-85 3.913362452278374 1101 86-87 3.734676105337787 1101 88-89 3.6922407223207117 1101 90-91 3.586511302812312 1101 92-93 3.5958265834451453 1101 94-95 3.402714146308597 1101 96-97 3.554669434972787 1101 98-99 3.303564283953733 1101 100 3.3793525257315338 1102 1 6.689880846904437 1102 2 10.276071077370695 1102 3 7.982455478524717 1102 4 12.079549050237986 1102 5 10.08523697948716 1102 6 9.371253110501367 1102 7 6.38063139067723 1102 8 7.628813761800679 1102 9 6.143129628009913 1102 10-11 7.203665755042337 1102 12-13 8.648016927565397 1102 14-15 5.839696905955787 1102 16-17 6.920212040765172 1102 18-19 7.248533775292568 1102 20-21 6.856757805313002 1102 22-23 6.370568093213123 1102 24-25 6.613296966106798 1102 26-27 8.295991923702548 1102 28-29 7.499253645896136 1102 30-31 3.79297618004896 1102 32-33 4.267923889189117 1102 34-35 5.078838417195708 1102 36-37 4.950084898983942 1102 38-39 4.521747769795727 1102 40-41 6.079993364782329 1102 42-43 6.421991930842012 1102 44-45 5.894299722854424 1102 46-47 4.727922884262469 1102 48-49 3.2531284396029925 1102 50-51 4.87107060777323 1102 52-53 5.454784775366285 1102 54-55 4.911135499502187 1102 56-57 6.043243749839334 1102 58-59 5.652339144686895 1102 60-61 5.453518903422957 1102 62-63 3.81307694786409 1102 64-65 5.661241636778836 1102 66-67 3.44157910966193 1102 68-69 5.478088677651556 1102 70-71 4.883857854042468 1102 72-73 4.7102868598023235 1102 74-75 3.9036966817747683 1102 76-77 2.868469314057716 1102 78-79 3.8957166526876392 1102 80-81 4.044003832306604 1102 82-83 3.495606591046162 1102 84-85 2.613918449101245 1102 86-87 2.757908829722215 1102 88-89 2.7524075213675765 1102 90-91 2.9254707944723606 1102 92-93 2.8430465993307656 1102 94-95 2.7579564020671334 1102 96-97 2.9549871474430027 1102 98-99 2.6945491926113974 1102 100 2.783244503491659 1103 1 6.699694731296507 1103 2 10.716951654916098 1103 3 7.573255272796008 1103 4 11.5614569129839 1103 5 10.068182732729415 1103 6 8.81370726396057 1103 7 7.863540793837583 1103 8 7.854832831563819 1103 9 6.884758507065854 1103 10-11 7.314797840079848 1103 12-13 8.525526637660736 1103 14-15 8.368502512413858 1103 16-17 7.674100941522745 1103 18-19 7.107126137502636 1103 20-21 8.17727576847124 1103 22-23 6.785304910648662 1103 24-25 5.125677357597187 1103 26-27 5.6667528744038655 1103 28-29 6.878006273704141 1103 30-31 5.929977008129331 1103 32-33 7.829532734248172 1103 34-35 6.995020150386743 1103 36-37 3.915154224010749 1103 38-39 6.009525054757095 1103 40-41 6.340277408786331 1103 42-43 7.251735560729301 1103 44-45 5.069255217918627 1103 46-47 6.051103570634922 1103 48-49 5.496051254030405 1103 50-51 6.177239535531903 1103 52-53 5.8527253837965105 1103 54-55 4.706244595982639 1103 56-57 5.6118668055483845 1103 58-59 5.013399406426615 1103 60-61 4.6521267431152715 1103 62-63 2.427913951227108 1103 64-65 4.622990668798476 1103 66-67 3.744405105327342 1103 68-69 4.688877142813986 1103 70-71 3.4395255333033745 1103 72-73 4.040024750203003 1103 74-75 3.0295062283903995 1103 76-77 3.2340051796312856 1103 78-79 2.5825307242507485 1103 80-81 3.8598001908574346 1103 82-83 3.038016773660683 1103 84-85 3.06754626698989 1103 86-87 2.597463235112997 1103 88-89 2.765730065528942 1103 90-91 2.3178428254439076 1103 92-93 1.9268822440297626 1103 94-95 2.480244486669715 1103 96-97 2.7391524778190153 1103 98-99 2.5088402113670796 1103 100 2.427535834730506 1104 1 6.236320057184223 1104 2 9.537995012962654 1104 3 7.225704088532659 1104 4 12.133813808902197 1104 5 9.292024779431966 1104 6 8.177188062371552 1104 7 3.8329787312605923 1104 8 6.391288705663268 1104 9 6.5303303634650405 1104 10-11 6.80561248896592 1104 12-13 7.156866262921792 1104 14-15 7.0114662029068064 1104 16-17 6.9227550686479375 1104 18-19 6.645560177043347 1104 20-21 7.239326475650039 1104 22-23 6.156860782586989 1104 24-25 5.434597954617583 1104 26-27 7.914396021053168 1104 28-29 6.875563302826269 1104 30-31 6.011660219402803 1104 32-33 7.251940924123236 1104 34-35 7.768596951831164 1104 36-37 6.284334905786512 1104 38-39 6.936881482431797 1104 40-41 6.688094967986402 1104 42-43 6.993344586501038 1104 44-45 6.447744123819998 1104 46-47 6.107089459148412 1104 48-49 4.862162157033254 1104 50-51 5.149513129933418 1104 52-53 5.432464729290203 1104 54-55 4.163426994214969 1104 56-57 5.306823625630862 1104 58-59 3.2063460535078185 1104 60-61 3.64008346889414 1104 62-63 3.2854032782202687 1104 64-65 2.341243016729006 1104 66-67 2.6802944538007694 1104 68-69 3.689192061185704 1104 70-71 1.8426760595371334 1104 72-73 1.8699205316229666 1104 74-75 2.6237490097442198 1104 76-77 3.1194932629956718 1104 78-79 3.5779617473859098 1104 80-81 2.428418914837991 1104 82-83 2.3513553144302897 1104 84-85 2.593989668782836 1104 86-87 0.14982293721839213 1104 88-89 -0.4220259540207216 1104 90-91 1.8092741314898788 1104 92-93 2.0492211960678652 1104 94-95 1.9718331291104008 1104 96-97 2.4303056373499317 1104 98-99 2.06330831939448 1104 100 0.7373614843939258 1105 1 5.536082591761858 1105 2 -2.5533489565071115 1105 3 0.6200007431074255 1105 4 8.15018965193239 1105 5 8.104123647632903 1105 6 7.796629257542946 1105 7 5.0053841182375045 1105 8 5.790703376246157 1105 9 4.787862320860004 1105 10-11 4.653420347450599 1105 12-13 4.731181384107078 1105 14-15 5.505951945447343 1105 16-17 5.625366448577488 1105 18-19 5.791831463617399 1105 20-21 6.924751283307316 1105 22-23 1.6627896922186238 1105 24-25 3.8131351965469484 1105 26-27 6.5231766691499935 1105 28-29 5.474241232251405 1105 30-31 4.8464485750163995 1105 32-33 6.7049236417165865 1105 34-35 5.967700760680536 1105 36-37 5.848476295519774 1105 38-39 3.8613505139555535 1105 40-41 5.034202382706958 1105 42-43 4.943973526313101 1105 44-45 5.854025089172996 1105 46-47 4.872952432098465 1105 48-49 4.788918474047456 1105 50-51 5.717909122182505 1105 52-53 5.179477507645508 1105 54-55 4.77433761476804 1105 56-57 4.807299181125046 1105 58-59 1.7425259144148235 1105 60-61 3.8257043836045064 1105 62-63 -0.5371524684560995 1105 64-65 -0.2835756705471475 1105 66-67 0.6170320116055024 1105 68-69 2.234963003545026 1105 70-71 2.3954753767623984 1105 72-73 2.139085763239695 1105 74-75 2.2254604341882676 1105 76-77 1.3530087638363213 1105 78-79 2.6877299169591673 1105 80-81 1.0270304341630023 1105 82-83 0.3266830581372737 1105 84-85 0.8305129389476704 1105 86-87 -0.7425582626740379 1105 88-89 0.89376179798424 1105 90-91 0.8737331883481048 1105 92-93 1.309314380616275 1105 94-95 0.8335475748852375 1105 96-97 1.7049187163260058 1105 98-99 1.5776199425428068 1105 100 1.7276725137965059 1106 1 5.895290116722258 1106 2 10.040157552836511 1106 3 6.480481158596355 1106 4 11.197819793877414 1106 5 9.464219452180583 1106 6 9.250601420503745 1106 7 6.421893538878965 1106 8 7.373551332818014 1106 9 6.85782332384575 1106 10-11 5.638939111715061 1106 12-13 7.731206655789702 1106 14-15 7.636207412474452 1106 16-17 7.358480552069757 1106 18-19 6.8866710341624575 1106 20-21 7.996036843372728 1106 22-23 5.810510539447371 1106 24-25 4.700309488683107 1106 26-27 7.4384472959775785 1106 28-29 5.902670382968829 1106 30-31 5.129296868544024 1106 32-33 7.001577253754061 1106 34-35 6.487252137770625 1106 36-37 5.7681791743414905 1106 38-39 7.002002831441057 1106 40-41 6.2939324618819334 1106 42-43 5.854351394929878 1106 44-45 4.195835828498879 1106 46-47 5.654700404587466 1106 48-49 5.603966260793605 1106 50-51 6.212911265863006 1106 52-53 5.3021635842480705 1106 54-55 4.830201030228729 1106 56-57 4.564624679755404 1106 58-59 4.479294303214285 1106 60-61 4.2548708256339935 1106 62-63 4.540988216032638 1106 64-65 4.770602645161791 1106 66-67 2.649212651346513 1106 68-69 3.005253757863837 1106 70-71 2.3357260590176026 1106 72-73 2.113982638729084 1106 74-75 2.1889843304495322 1106 76-77 3.2211586135219576 1106 78-79 3.8097606948199534 1106 80-81 3.367707718466381 1106 82-83 1.6430007669920883 1106 84-85 2.4890499861112083 1106 86-87 3.0028955621911173 1106 88-89 3.022195778401013 1106 90-91 2.783808438593919 1106 92-93 2.35784973016456 1106 94-95 1.0507483003991425 1106 96-97 1.291426092769143 1106 98-99 1.791961518135139 1106 100 2.5238168412584656 1107 1 6.076171101098062 1107 2 10.182446577428298 1107 3 5.905733863096675 1107 4 11.275639369230902 1107 5 9.136822349172515 1107 6 7.974410167018981 1107 7 3.5554957192765215 1107 8 6.217456182902136 1107 9 5.552732930003316 1107 10-11 4.6032768579512435 1107 12-13 6.050074473929154 1107 14-15 6.038076568979207 1107 16-17 6.985141401769177 1107 18-19 6.228161780748135 1107 20-21 5.173368721391842 1107 22-23 5.475035409606974 1107 24-25 4.39860827128544 1107 26-27 7.2822458929968725 1107 28-29 6.423997362679319 1107 30-31 5.096171565447634 1107 32-33 4.814572198309431 1107 34-35 6.673917776054374 1107 36-37 6.452376185641739 1107 38-39 6.530709869644067 1107 40-41 7.66366747435799 1107 42-43 6.065514164126888 1107 44-45 3.821146629296482 1107 46-47 3.4380020623723375 1107 48-49 3.894796477370374 1107 50-51 4.700683781691751 1107 52-53 3.5968377911853544 1107 54-55 5.102732437110742 1107 56-57 5.089598891162936 1107 58-59 2.8904645397581596 1107 60-61 2.5889815983909443 1107 62-63 0.12735375502268909 1107 64-65 1.8362296178282556 1107 66-67 3.6171498788984167 1107 68-69 4.614758166477056 1107 70-71 3.8567785586992684 1107 72-73 2.1460584494018704 1107 74-75 2.1986995011182024 1107 76-77 2.8822274228374134 1107 78-79 3.760960651743016 1107 80-81 1.9789304634969547 1107 82-83 1.3619362653706055 1107 84-85 2.200419250308311 1107 86-87 2.1513523443708102 1107 88-89 2.365285231459339 1107 90-91 1.1446381742803204 1107 92-93 2.196723942113941 1107 94-95 2.19935527615414 1107 96-97 2.242310507721985 1107 98-99 1.6451631304047538 1107 100 0.01109492051461558 1108 1 4.102895660134379 1108 2 -3.041310849427129 1108 3 0.19116124310420446 1108 4 6.242702838249258 1108 5 6.450913741894183 1108 6 6.545840606972039 1108 7 3.85195780767452 1108 8 4.788351925887639 1108 9 4.644054498259408 1108 10-11 3.120921945078422 1108 12-13 2.2690252087363767 1108 14-15 3.6012055158790552 1108 16-17 4.246780957764901 1108 18-19 3.607257483657527 1108 20-21 4.565152403158901 1108 22-23 4.0294240840764495 1108 24-25 2.4287241275269444 1108 26-27 4.207259090385332 1108 28-29 5.89926352963278 1108 30-31 4.327838008015927 1108 32-33 4.16696331048087 1108 34-35 2.799754504434418 1108 36-37 1.0004247073859958 1108 38-39 2.289217803511157 1108 40-41 4.924928853454134 1108 42-43 2.6329985544110244 1108 44-45 3.101060491812394 1108 46-47 4.093553973059457 1108 48-49 4.473270843568734 1108 50-51 1.634265559023774 1108 52-53 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2213 52-53 -17.091202601046923 2213 54-55 -16.899548732159026 2213 56-57 -16.52145345920875 2213 58-59 -17.04970237021428 2213 60-61 -18.008699670727346 2213 62-63 -16.429085053653715 2213 64-65 -16.500216797903477 2213 66-67 -8.091969540502323 2213 68-69 -21.395024904556546 2213 70-71 -16.253568268078705 2213 72-73 -8.619752005136265 2213 74-75 -3.9075562835121396 2213 76-77 -2.869848649610553 2213 78-79 -4.331197449960378 2213 80-81 -2.9855911019838786 2213 82-83 -2.816057224581545 2213 84-85 -2.779496181593384 2213 86-87 -3.2740494890905723 2213 88-89 -2.5708803958290503 2213 90-91 -2.6542354983927297 2213 92-93 -3.180811466691864 2213 94-95 -3.317570185124243 2213 96-97 -3.223910093093288 2213 98-99 -3.5338075298554372 2213 100 -3.1167751324126804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 326.0 3 329.0 4 1855.0 5 5856.0 6 9087.0 7 10497.0 8 10379.0 9 10624.0 10 12553.0 11 15270.0 12 19649.0 13 24026.0 14 22321.0 15 24339.0 16 25846.0 17 26346.0 18 26192.0 19 25863.0 20 25678.0 21 25727.0 22 27000.0 23 29089.0 24 31257.0 25 33217.0 26 35597.0 27 38070.0 28 40262.0 29 42531.0 30 45639.0 31 51433.0 32 58408.0 33 67796.0 34 75612.0 35 79505.0 36 72366.0 37 46790.0 38 9502.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.327090547873134 18.684578356773226 12.589715501739343 25.3986155936143 2 16.022994147994147 21.74963424963425 35.66631535381536 26.56105624855625 3 20.387051467311302 26.399834853924826 26.94688122794596 26.266232450817917 4 14.116207652597051 18.381593425426626 32.38881355814043 35.113385363835896 5 14.140598278270689 38.152499556998805 32.818765118703716 14.888137046026795 6 32.95448838353908 33.41271834225619 18.026356760448788 15.60643651375594 7 27.667642847717726 31.36190057920951 21.13701131043936 19.833445262633404 8 27.912419893223984 33.08511375905071 19.950970195822066 19.051496151903248 9 27.189874290046 16.84880922983271 19.103050768291304 36.858265711829986 10-11 24.706694976385883 27.503203314725916 25.518509228851805 22.271592480036396 12-13 26.33181607610077 25.08814697369167 25.166042548450097 23.413994401757467 14-15 24.960525067926216 25.853220714488938 23.020069397663868 26.16618481992098 16-17 23.41961690839631 28.397311341292376 25.599164192365492 22.583907557945825 18-19 24.411620311239663 27.73259456439326 26.010521841099475 21.8452632832676 20-21 24.33894982589037 27.139233523614386 25.650263750227797 22.871552900267446 22-23 23.60733059246399 27.20916535436097 24.222104576431917 24.961399476743125 24-25 23.980180965544665 27.77326925172487 23.561843913872178 24.684705868858288 26-27 24.59095752258914 26.018191705176392 24.460429128399408 24.930421643835064 28-29 24.405997314188266 25.808002636521916 25.794133835380713 23.991866213909102 30-31 25.210898805919207 26.771916339179647 24.851951283734948 23.165233571166205 32-33 23.13941549595065 26.780352227637238 25.50899832783226 24.57123394857985 34-35 22.640583412816813 27.056875731373935 25.583576088964318 24.718964766844934 36-37 25.08729138673054 26.366477770637374 24.619140815174 23.927090027458085 38-39 25.31922457378919 25.77799850479391 24.524318293923606 24.378458627493295 40-41 23.97059186098688 26.820972283174143 24.46797599617359 24.740459859665386 42-43 25.29550804526676 26.23095462815308 24.59793036620286 23.875606960377297 44-45 25.785969906171243 25.566850876790248 24.501702352847364 24.145476864191146 46-47 24.270174062043857 26.075555735047224 26.39556373524723 23.258706467661693 48-49 24.973380196750973 25.888771625645475 25.767404168708303 23.370444008895255 50-51 23.803481169666806 26.11918965223189 25.77726483402329 24.300064344078017 52-53 23.252647661508774 26.486187803412438 24.35777503549487 25.903389499583916 54-55 23.705477687104576 26.96633120280679 23.95306268549227 25.375128424596365 56-57 24.70046920307779 25.449795651214295 24.191024255477842 25.658710890230076 58-59 24.445951093666466 24.949174016267126 25.45511453006991 25.149760359996492 60-61 25.022249841812933 25.86065309233932 24.882036968895452 24.235060096952303 62-63 23.258180679505244 26.10329202919621 25.431633824658718 25.206893466639823 64-65 22.688349669235905 26.355345709170358 25.574475025793532 25.381829595800205 66-67 24.467568182768375 25.75996320848063 24.73379570486884 25.03867290388215 68-69 25.486191262710552 25.144320533116282 24.179372051996374 25.190116152176795 70-71 24.10632876638633 25.91347442605525 24.554660057563755 25.425536749994667 72-73 25.188625025018407 25.36049460245061 24.3714182361701 25.07946213636088 74-75 25.477952087853375 24.93470400593032 24.258926369278306 25.328417536937998 76-77 24.25874643562185 25.465699714849748 25.76565584558017 24.509898003948237 78-79 24.891731826684538 25.384262988389978 25.43705880001415 24.286946384911335 80-81 23.914046755620173 25.590965549707327 25.389622460899027 25.105365233773476 82-83 23.44716106103622 25.851454018500515 24.422410986474087 26.278973933989175 84-85 23.772302191986867 26.11159496819116 23.79580164450449 26.32030119531748 86-87 24.57417015037833 24.884504178184475 24.400052652840756 26.14127301859644 88-89 24.4265494919691 24.474073642878515 25.40823018242907 25.69114668272331 90-91 24.88764263702708 25.099136186270083 24.84240991262251 25.170811264080328 92-93 23.33777375759088 25.44897581108705 25.09309264150285 26.120157789819213 94-95 22.744299407858147 25.553020141546984 25.6618430187966 26.04083743179827 96-97 24.5229815515318 25.023392163488968 24.853560514391404 25.600065770587825 98-99 24.692520561814842 24.572248417143207 24.631635342485033 26.103595678556918 100 23.575255193397567 25.02359501970558 25.449257334855265 25.95189245204159 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 425.0 1 489.0 2 514.5 3 438.5 4 385.5 5 354.5 6 349.0 7 411.5 8 517.5 9 664.5 10 817.5 11 984.0 12 1228.5 13 1550.0 14 1901.5 15 2200.0 16 2528.5 17 2886.0 18 3242.5 19 3547.5 20 3809.0 21 4081.5 22 4437.5 23 4814.0 24 5280.0 25 5929.0 26 6681.0 27 7723.0 28 8825.0 29 9721.0 30 10632.0 31 11715.5 32 12887.5 33 14066.0 34 15404.0 35 16872.5 36 18615.5 37 20474.0 38 22145.5 39 23790.0 40 25358.0 41 26779.5 42 28010.0 43 29458.0 44 30998.0 45 32514.0 46 34938.0 47 38212.0 48 42071.0 49 48733.5 50 58327.0 51 58492.0 52 48143.5 53 39710.0 54 34918.0 55 31714.5 56 29166.5 57 27153.0 58 25542.5 59 24067.0 60 22141.5 61 19749.5 62 17346.5 63 14884.0 64 12518.0 65 10521.5 66 8792.5 67 7232.5 68 5788.0 69 4556.5 70 3655.5 71 2932.0 72 2322.0 73 1837.5 74 1425.5 75 1066.0 76 803.0 77 596.5 78 420.0 79 290.5 80 177.5 81 113.5 82 73.5 83 43.5 84 32.5 85 22.5 86 21.5 87 18.5 88 8.0 89 5.0 90 4.5 91 3.0 92 2.5 93 2.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.956676821665925 2 24.906490887604757 3 6.341092330987926 4 22.242971909320556 5 12.304534333654967 6 10.053268572626314 7 8.250665857157829 8 8.786604074286892 9 5.606752171037653 10-11 7.0671205698363995 12-13 9.99585307414595 14-15 7.593030273462205 16-17 8.075663779177553 18-19 6.697872509585813 20-21 8.28291972491155 22-23 5.967869004123435 24-25 5.482073354510663 26-27 9.949550252790818 28-29 6.518307909696398 30-31 4.442224920585015 32-33 9.013736353090408 34-35 7.3135419264142 36-37 7.211856413369906 38-39 6.221292250759818 40-41 8.387722208369022 42-43 6.716619505549111 44-45 6.527252259577682 46-47 6.041501783449158 48-49 4.120183151374539 50-51 6.7662651647386625 52-53 4.676539783384109 54-55 5.159128115615209 56-57 5.032326145328519 58-59 3.5888074561546164 60-61 4.1198669369847964 62-63 3.056257250344222 64-65 4.7265920039319 66-67 2.7563956618999605 68-69 5.205521283938839 70-71 2.5961201397848295 72-73 1.5942626061125145 74-75 0.9131819840917057 76-77 1.1459609484263364 78-79 1.6885396677399886 80-81 2.313334128386656 82-83 0.7920266993361305 84-85 1.192715504623958 86-87 0.4778451163849648 88-89 0.6688386077893542 90-91 0.2308365045119276 92-93 0.2683756699227714 94-95 0.581111701377972 96-97 0.5464184654748094 98-99 0.5051750743555551 100 0.348468257496088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1106844.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.78064035512547 #Duplication Level Percentage of deduplicated Percentage of total 1 94.86436519625059 80.42661628220603 2 3.1728256553041865 5.37988381583719 3 0.8343006668103932 2.121976343426799 4 0.35324370325918664 1.1979290945491905 5 0.203609919296288 0.8631089670297357 6 0.12198647051339939 0.6205254650872579 7 0.09036204137444254 0.5362666212065106 8 0.06749025205409155 0.4577493429499762 9 0.0471324110127907 0.3596324388430826 >10 0.22610514316271846 3.2351044664200934 >50 0.010161107908218265 0.5801524746159397 >100 0.005827458941499121 1.014917122697595 >500 0.0014029026441138024 0.7641372298025662 >1k 9.712402920787862E-4 1.3444679827524795 >5k 2.1583117601750804E-4 1.0975323525756204 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6810 0.6152628554701475 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5186 0.4685393786296894 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2354 0.212676763843866 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2299 0.20770768057648595 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2014 0.1819587945546075 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1601 0.14464549656500825 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1516 0.13696600424269365 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1435 0.1296478997943703 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1282 0.11582481361420399 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1167 0.10543491223695481 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.5173484248909515E-5 0.0 24-25 0.0 0.0 0.0 1.8069393699563806E-4 0.0 26-27 0.0 0.0 0.0 4.065613582401856E-4 0.0 28-29 0.0 0.0 0.0 0.001761765885707471 0.0 30-31 9.034696849781903E-5 0.0 0.0 0.006459808247594061 4.5173484248909515E-5 32-33 9.034696849781903E-5 0.0 0.0 0.020779802754498377 9.034696849781903E-5 34-35 9.034696849781903E-5 0.0 0.0 0.04521865773315842 9.034696849781903E-5 36-37 1.3552045274672854E-4 0.0 0.0 0.08045397544730784 9.034696849781903E-5 38-39 1.8069393699563806E-4 0.0 0.0 0.13809534134891638 9.034696849781903E-5 40-41 1.8069393699563806E-4 0.0 0.0 0.22351840006360427 9.034696849781903E-5 42-43 1.8069393699563806E-4 0.0 0.0 0.2881164825395449 9.034696849781903E-5 44-45 1.8069393699563806E-4 0.0 0.0 0.35935506720007515 9.034696849781903E-5 46-47 1.8069393699563806E-4 0.0 0.0 0.45006342357188545 9.034696849781903E-5 48-49 1.8069393699563806E-4 0.0 0.0 0.5364351254558004 9.034696849781903E-5 50-51 1.8069393699563806E-4 0.0 0.0 0.6337839840122005 9.034696849781903E-5 52-53 1.8069393699563806E-4 0.0 0.0 0.7386316409539195 9.034696849781903E-5 54-55 1.8069393699563806E-4 0.0 0.0 0.8482676872260229 9.034696849781903E-5 56-57 1.8069393699563806E-4 0.0 0.0 0.9709136969618122 9.034696849781903E-5 58-59 1.8069393699563806E-4 0.0 0.0 1.1091445587634752 9.034696849781903E-5 60-61 1.8069393699563806E-4 0.0 0.0 1.2451167463526929 9.034696849781903E-5 62-63 1.8069393699563806E-4 0.0 0.0 1.3869614868942688 9.034696849781903E-5 64-65 1.8069393699563806E-4 0.0 0.0 1.5554134096584522 9.034696849781903E-5 66-67 1.8069393699563806E-4 0.0 0.0 1.7439675329134006 9.034696849781903E-5 68-69 1.8069393699563806E-4 0.0 0.0 1.9645496565008258 9.034696849781903E-5 70-71 1.8069393699563806E-4 0.0 0.0 2.282435465160402 9.034696849781903E-5 72-73 1.8069393699563806E-4 0.0 0.0 2.5612913834289204 9.034696849781903E-5 74-75 1.8069393699563806E-4 0.0 0.0 2.835178218430059 9.034696849781903E-5 76-77 1.8069393699563806E-4 0.0 0.0 3.1405961454369358 9.034696849781903E-5 78-79 1.8069393699563806E-4 0.0 0.0 3.4608309752774558 9.034696849781903E-5 80-81 1.8069393699563806E-4 0.0 0.0 3.821405726552251 9.034696849781903E-5 82-83 1.8069393699563806E-4 0.0 0.0 4.273501956915338 9.034696849781903E-5 84-85 1.8069393699563806E-4 9.034696849781903E-5 0.0 4.712678570783236 9.034696849781903E-5 86-87 1.8069393699563806E-4 9.034696849781903E-5 0.0 5.16075436104817 9.034696849781903E-5 88 1.8069393699563806E-4 9.034696849781903E-5 0.0 5.544412762774158 9.034696849781903E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATA 375 0.0 25.897825 1 GTATAGG 235 2.397628E-7 23.246052 1 GTATATA 170 3.4366982E-4 21.422832 1 TGTACTA 355 1.8189894E-12 21.05103 5 GCTCTAG 340 1.9401341E-8 19.637596 1 CGTGTCG 130 0.0057206866 19.174162 9 TAAATAG 350 6.9303496E-10 18.504856 5 TCTATAG 190 0.0014212776 17.348171 3 TACATCG 385 8.078132E-8 17.337769 2 GTACTAT 320 9.34142E-8 17.11947 6 GTTCTAA 330 6.2045856E-6 16.554008 1 TAATACA 465 4.620233E-10 16.54081 4 ATGTACC 835 0.0 16.44471 94 TACTATC 220 4.0774996E-4 15.794523 7 CATGGGG 2975 0.0 15.696893 4 TCTACAC 420 3.9332008E-8 15.695966 3 ACTGATC 295 1.3908648E-5 15.204445 8 ATAGACG 290 8.93081E-5 15.154725 3 CTATGCG 235 6.514202E-4 14.849775 9 GTTTAAC 330 1.1477456E-4 14.714675 1 >>END_MODULE