##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295125_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1106844 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.202114299756786 33.0 33.0 33.0 27.0 33.0 2 29.771208950854863 33.0 33.0 33.0 22.0 33.0 3 30.668881070864547 33.0 33.0 33.0 27.0 33.0 4 34.965732298318464 37.0 37.0 37.0 33.0 37.0 5 35.35043511100029 37.0 37.0 37.0 33.0 37.0 6 35.08074127880713 37.0 37.0 37.0 33.0 37.0 7 34.950502509838785 37.0 37.0 37.0 33.0 37.0 8 34.948125481097605 37.0 37.0 37.0 33.0 37.0 9 34.886354355265965 37.0 37.0 37.0 33.0 37.0 10-11 34.853553888352835 37.0 37.0 37.0 33.0 37.0 12-13 34.7549044851849 37.0 37.0 37.0 30.0 37.0 14-15 36.08302389496623 37.0 37.0 40.0 27.0 40.0 16-17 36.040372446342936 37.0 37.0 40.0 27.0 40.0 18-19 36.06992584320825 37.0 37.0 40.0 27.0 40.0 20-21 36.08358856351934 37.0 37.0 40.0 27.0 40.0 22-23 36.07191528345458 37.0 37.0 40.0 27.0 40.0 24-25 36.028714525262814 37.0 37.0 40.0 27.0 40.0 26-27 35.93715013136449 37.0 37.0 40.0 27.0 40.0 28-29 35.85421748683645 37.0 37.0 40.0 27.0 40.0 30-31 35.68878541149431 37.0 37.0 40.0 27.0 40.0 32-33 35.58916523014987 37.0 37.0 40.0 27.0 40.0 34-35 35.49530557151685 37.0 37.0 40.0 27.0 40.0 36-37 35.45322556746931 37.0 37.0 40.0 27.0 40.0 38-39 35.34327646895136 37.0 33.0 40.0 27.0 40.0 40-41 35.2138539848434 37.0 33.0 40.0 27.0 40.0 42-43 35.02890289869214 37.0 33.0 40.0 27.0 40.0 44-45 34.81182533401274 37.0 33.0 40.0 22.0 40.0 46-47 34.564770193450926 37.0 33.0 40.0 22.0 40.0 48-49 34.35978602223981 37.0 33.0 40.0 22.0 40.0 50-51 34.232024567147675 37.0 33.0 40.0 22.0 40.0 52-53 34.13933490175671 37.0 33.0 40.0 22.0 40.0 54-55 33.9698046879235 37.0 33.0 40.0 22.0 40.0 56-57 33.76128478810023 37.0 33.0 40.0 22.0 40.0 58-59 33.60870502076173 37.0 33.0 40.0 22.0 40.0 60-61 33.37536635695726 37.0 33.0 37.0 22.0 40.0 62-63 33.13012944913646 37.0 33.0 37.0 22.0 40.0 64-65 32.954755593380824 37.0 33.0 37.0 22.0 40.0 66-67 32.68932568636592 37.0 33.0 37.0 22.0 40.0 68-69 32.44581711605249 37.0 33.0 37.0 22.0 40.0 70-71 32.16713105008475 37.0 33.0 37.0 22.0 37.0 72-73 31.91246056354825 35.0 33.0 37.0 22.0 37.0 74-75 31.692722732381437 33.0 30.0 37.0 22.0 37.0 76-77 31.4606209185757 33.0 27.0 37.0 15.0 37.0 78-79 31.253497331150548 33.0 27.0 37.0 15.0 37.0 80-81 31.144476095999075 33.0 27.0 37.0 15.0 37.0 82-83 31.018245118553292 33.0 27.0 37.0 15.0 37.0 84-85 30.846895316774543 33.0 27.0 37.0 15.0 37.0 86-87 30.885026254829047 33.0 27.0 37.0 15.0 37.0 88-89 30.951956192561916 33.0 27.0 37.0 15.0 37.0 90-91 31.01802150980626 33.0 27.0 37.0 15.0 37.0 92-93 31.01067359085833 33.0 27.0 37.0 15.0 37.0 94-95 31.109981623426606 33.0 27.0 37.0 15.0 37.0 96-97 31.092904239441147 33.0 27.0 37.0 15.0 37.0 98-99 31.028325129828595 33.0 27.0 37.0 15.0 37.0 100 30.416198669369848 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6553359672889378 1101 2 4.027408482455023 1101 3 2.287294752462646 1101 4 1.1551900239046944 1101 5 0.43248637874194884 1101 6 0.33560991391426853 1101 7 0.42170584131888234 1101 8 0.32803709689014227 1101 9 0.519045972375082 1101 10-11 0.44378441671692315 1101 12-13 0.5049212833114467 1101 14-15 0.8740949206105881 1101 16-17 0.8318309373897961 1101 18-19 0.8883772798482141 1101 20-21 0.7992658865899003 1101 22-23 0.8988250434812457 1101 24-25 0.8204615032081577 1101 26-27 0.7592646269536871 1101 28-29 0.726551721258808 1101 30-31 0.5273794437118013 1101 32-33 0.9217840535188628 1101 34-35 1.0268093966524816 1101 36-37 1.0539610441632945 1101 38-39 0.8617014449224456 1101 40-41 0.9337867152054429 1101 42-43 0.9547503832871698 1101 44-45 0.775497908179581 1101 46-47 0.8350730538465854 1101 48-49 0.8397139640364983 1101 50-51 0.8393261664771856 1101 52-53 0.921736029367672 1101 54-55 0.9530017511888076 1101 56-57 0.78631293965352 1101 58-59 0.812232681495999 1101 60-61 0.799652143932903 1101 62-63 0.8920123023399711 1101 64-65 0.8622518197028057 1101 66-67 0.7912194107472104 1101 68-69 0.7685960727892507 1101 70-71 0.6519864511954054 1101 72-73 0.6770572651852191 1101 74-75 0.770737433332183 1101 76-77 0.7517290026162016 1101 78-79 0.8753001776789198 1101 80-81 0.7975304520420643 1101 82-83 0.9375299223173386 1101 84-85 0.8065001695314358 1101 86-87 0.8693276432398882 1101 88-89 0.8623336322726658 1101 90-91 0.7887229400725317 1101 92-93 0.849495325585643 1101 94-95 0.796083455883295 1101 96-97 0.7390695251712138 1101 98-99 0.7251618211359876 1101 100 0.8172359745285682 1102 1 0.49211118571626145 1102 2 3.923754789047557 1102 3 2.23884360075494 1102 4 1.13970154098174 1102 5 0.5555999609500475 1102 6 0.4761976819841678 1102 7 0.5047082241624068 1102 8 0.49205615963835214 1102 9 0.4153922789676159 1102 10-11 0.6250790950330511 1102 12-13 0.6484876057896045 1102 14-15 0.9747700596097957 1102 16-17 0.7889397539108458 1102 18-19 0.8029920534780857 1102 20-21 0.6531181502912489 1102 22-23 0.825949745625806 1102 24-25 0.7884916858928293 1102 26-27 0.7787245157543197 1102 28-29 0.8031998546980432 1102 30-31 0.8288091498277694 1102 32-33 0.9031184458937602 1102 34-35 0.937055623816903 1102 36-37 0.7950253809385188 1102 38-39 0.7455378230002907 1102 40-41 0.9180996619886059 1102 42-43 0.9801673809043336 1102 44-45 0.9188656603638563 1102 46-47 0.8477815526551638 1102 48-49 0.746187355617117 1102 50-51 0.7579123459847281 1102 52-53 0.7575183963255796 1102 54-55 0.8574894398305801 1102 56-57 0.7603002311547158 1102 58-59 0.612073825260893 1102 60-61 0.7369039310655552 1102 62-63 0.7436802928085982 1102 64-65 0.7262311683922462 1102 66-67 0.793800824567704 1102 68-69 0.6301925779520801 1102 70-71 0.6073081350714986 1102 72-73 0.6768586948913367 1102 74-75 0.5634300465172508 1102 76-77 0.6522452853802996 1102 78-79 0.5089379854628753 1102 80-81 0.586053089055568 1102 82-83 0.684948508496845 1102 84-85 0.596214228308245 1102 86-87 0.5960949188554601 1102 88-89 0.6852089301281055 1102 90-91 0.6427737740677699 1102 92-93 0.6689949284450591 1102 94-95 0.6259087140246784 1102 96-97 0.659839977911485 1102 98-99 0.6604279053297937 1102 100 0.793407539262482 1103 1 0.5805082076879735 1103 2 3.9697653835021285 1103 3 2.2761180588597156 1103 4 1.127701709340009 1103 5 0.5209382446968718 1103 6 0.6337306465661143 1103 7 0.549514801869158 1103 8 0.4974350151783469 1103 9 0.6125243531638702 1103 10-11 0.5778400910125399 1103 12-13 0.6340278461274167 1103 14-15 0.8966289422146616 1103 16-17 0.9407397685146464 1103 18-19 1.0122030366872181 1103 20-21 0.9005472588628862 1103 22-23 0.8322021191426643 1103 24-25 0.839633381381816 1103 26-27 0.9264919032898788 1103 28-29 0.9402009219236902 1103 30-31 0.7717993075647982 1103 32-33 0.9082740991764737 1103 34-35 1.0614976580312572 1103 36-37 0.908523011327695 1103 38-39 0.7668710961752936 1103 40-41 0.815423993043467 1103 42-43 0.850625733116857 1103 44-45 0.8181395834209368 1103 46-47 0.784161495827874 1103 48-49 0.7186664375798557 1103 50-51 0.7427243889581732 1103 52-53 0.6846397504362827 1103 54-55 0.6476169010739028 1103 56-57 0.6635752938187451 1103 58-59 0.5444071912242663 1103 60-61 0.5630104801705329 1103 62-63 0.6774417546430911 1103 64-65 0.5671959729037326 1103 66-67 0.7530899285668795 1103 68-69 0.7725717832818901 1103 70-71 0.6886313992195738 1103 72-73 0.5752582231055356 1103 74-75 0.5596136067781963 1103 76-77 0.6525446155503261 1103 78-79 0.7172272230645511 1103 80-81 0.5731336641138647 1103 82-83 0.5255651174869875 1103 84-85 0.5944231099086927 1103 86-87 0.6370743750008394 1103 88-89 0.6862681045539745 1103 90-91 0.7837430585674241 1103 92-93 0.7973789536681863 1103 94-95 0.5732069144724221 1103 96-97 0.580428600527167 1103 98-99 0.5827166117100937 1103 100 0.7080181677075679 1104 1 0.42306703119812283 1104 2 3.352451209081064 1104 3 1.9501321114064716 1104 4 0.9663457872374082 1104 5 0.3670223202890739 1104 6 0.43501232422722325 1104 7 0.22477755855996406 1104 8 0.21517434168034555 1104 9 0.32259872858258376 1104 10-11 0.40225455731635407 1104 12-13 0.4032490817272887 1104 14-15 0.5355236614093002 1104 16-17 0.5944927899955701 1104 18-19 0.5881642621887408 1104 20-21 0.4819929920504862 1104 22-23 0.5098650335300619 1104 24-25 0.6608030505922784 1104 26-27 0.5840964738712131 1104 28-29 0.441270023544341 1104 30-31 0.4564712227072718 1104 32-33 0.6668278944708561 1104 34-35 0.6714072804697508 1104 36-37 0.6025701127034111 1104 38-39 0.63821527616944 1104 40-41 0.623449851877524 1104 42-43 0.6817197924482272 1104 44-45 0.6137410243014187 1104 46-47 0.6539032513025518 1104 48-49 0.6345686611147627 1104 50-51 0.5579698763015202 1104 52-53 0.45577399030666044 1104 54-55 0.5716486355463886 1104 56-57 0.6366043812414617 1104 58-59 0.7006288878639779 1104 60-61 0.6795036729565709 1104 62-63 0.7401682224557575 1104 64-65 0.7129564365107228 1104 66-67 0.7473517343630149 1104 68-69 0.6326923609176589 1104 70-71 0.7034961685064616 1104 72-73 0.70856029654054 1104 74-75 0.614696652889382 1104 76-77 0.7511624456112997 1104 78-79 0.767238094174445 1104 80-81 0.6674374317393053 1104 82-83 0.7202245374019434 1104 84-85 0.7408056334862287 1104 86-87 0.6143909201097237 1104 88-89 0.6119147882433147 1104 90-91 0.7831987663257962 1104 92-93 0.7414619100599751 1104 94-95 0.7368537920080591 1104 96-97 0.6376856230349475 1104 98-99 0.7055731693796936 1104 100 0.6171564816246189 1105 1 0.24682909104216932 1105 2 3.5635753282431395 1105 3 2.1424439258947885 1105 4 0.8026213838927063 1105 5 0.21000056999499606 1105 6 0.15773799199393324 1105 7 0.18162595219169475 1105 8 0.14789614319568045 1105 9 0.10793071004299293 1105 10-11 0.2501201136850568 1105 12-13 0.2044430498491252 1105 14-15 0.32828593034653863 1105 16-17 0.2566641175334752 1105 18-19 0.39809033656936776 1105 20-21 0.3264771400910931 1105 22-23 0.32275901473665414 1105 24-25 0.25802423173207245 1105 26-27 0.35802761646026937 1105 28-29 0.3342129969941112 1105 30-31 0.30984388594297485 1105 32-33 0.35997098588074294 1105 34-35 0.6248972791744194 1105 36-37 0.4203517293356569 1105 38-39 0.32716894230285476 1105 40-41 0.3997706074009031 1105 42-43 0.4866019868483491 1105 44-45 0.3974567252929049 1105 46-47 0.3980947510727475 1105 48-49 0.5195162037109213 1105 50-51 0.32220350045849244 1105 52-53 0.3928654613429856 1105 54-55 0.47829165101205007 1105 56-57 0.4846221548198457 1105 58-59 0.5269210245793658 1105 60-61 0.45482326334432344 1105 62-63 0.5067760076920749 1105 64-65 0.5227768136280417 1105 66-67 0.3718054948513654 1105 68-69 0.32718847592680333 1105 70-71 0.4941118624624252 1105 72-73 0.4116772315763413 1105 74-75 0.45044170846017195 1105 76-77 0.4019592353076078 1105 78-79 0.4974416378436892 1105 80-81 0.48901568420683716 1105 82-83 0.37160578529375243 1105 84-85 0.46888232794650975 1105 86-87 0.43177876101216484 1105 88-89 0.4971369300282191 1105 90-91 0.3948557607887686 1105 92-93 0.3961699094524249 1105 94-95 0.3299801493877652 1105 96-97 0.4941569585289116 1105 98-99 0.3599305431692734 1105 100 0.44059158679257493 1106 1 0.4595295315877692 1106 2 3.93110726523177 1106 3 2.171243695866316 1106 4 0.9189043381668469 1106 5 0.2519161025648202 1106 6 0.1513935962422721 1106 7 0.18969700557319413 1106 8 0.05162788019303832 1106 9 0.1513314152753651 1106 10-11 0.10367761245917251 1106 12-13 0.2542228091407637 1106 14-15 0.22920216121707426 1106 16-17 0.1816444457348041 1106 18-19 0.2709365473894323 1106 20-21 0.22650773888396003 1106 22-23 0.11273536685992269 1106 24-25 0.18332041585240688 1106 26-27 0.25077764405855163 1106 28-29 0.26180031988374 1106 30-31 0.10977062213163435 1106 32-33 0.3117430683572451 1106 34-35 0.3818526078250386 1106 36-37 0.2682846340612528 1106 38-39 0.2903353445394714 1106 40-41 0.28922801554996624 1106 42-43 0.37916877469072574 1106 44-45 0.20671432829868053 1106 46-47 0.12376991415933958 1106 48-49 0.020952517787073077 1106 50-51 0.08999981936113244 1106 52-53 -0.028468042714600017 1106 54-55 0.12385156880768022 1106 56-57 0.08665003486102307 1106 58-59 -0.02233059665924486 1106 60-61 0.16397235054719772 1106 62-63 0.0643189872025971 1106 64-65 0.05092537646355311 1106 66-67 0.06826907988104125 1106 68-69 0.018985624703368842 1106 70-71 0.15740776803264822 1106 72-73 0.10229776290103132 1106 74-75 0.05411858584934848 1106 76-77 0.2608347786086753 1106 78-79 0.2275197698635978 1106 80-81 0.20008951089475957 1106 82-83 0.13131492507455889 1106 84-85 0.05749238861138295 1106 86-87 0.07971397629159682 1106 88-89 0.2358669629921195 1106 90-91 0.16934883490363717 1106 92-93 0.25071861977828647 1106 94-95 0.23799026191173667 1106 96-97 0.16646915863827516 1106 98-99 0.1560198345224073 1106 100 0.12645735131633273 1107 1 0.5915628918329006 1107 2 3.5934976764133495 1107 3 2.118722340270242 1107 4 0.8982929368658645 1107 5 0.4621933731123846 1107 6 0.17940105749334379 1107 7 0.06961207762547161 1107 8 0.08999852881254355 1107 9 0.2367031706200251 1107 10-11 0.13950504529030638 1107 12-13 0.1945559984327545 1107 14-15 0.19381049294744912 1107 16-17 0.05912187775317079 1107 18-19 0.1635894569793379 1107 20-21 0.438509187650844 1107 22-23 0.2571290487733009 1107 24-25 0.33171666341747397 1107 26-27 0.20061417519993086 1107 28-29 0.12059880176026638 1107 30-31 0.0676055114726708 1107 32-33 0.4385759548915047 1107 34-35 0.5987679433510493 1107 36-37 0.397616457218227 1107 38-39 0.13475088664515056 1107 40-41 0.15493709516644572 1107 42-43 0.2538580585569221 1107 44-45 0.37768939447190064 1107 46-47 0.3098346934780807 1107 48-49 0.18770760167873135 1107 50-51 0.11432262751703348 1107 52-53 0.042790257724021785 1107 54-55 0.1006823048815022 1107 56-57 0.09902474937469918 1107 58-59 0.09890973812171922 1107 60-61 0.23941422783481414 1107 62-63 0.3761214421817769 1107 64-65 0.19443753892593207 1107 66-67 0.31326282491973956 1107 68-69 0.31321670132884094 1107 70-71 0.3573078816853297 1107 72-73 0.2665291036775521 1107 74-75 0.31462138381850124 1107 76-77 0.24406626703885692 1107 78-79 0.1555375092995881 1107 80-81 0.28399971511738187 1107 82-83 0.39471720408850075 1107 84-85 0.3498320760191014 1107 86-87 0.2903122072923665 1107 88-89 0.13042709392976448 1107 90-91 0.1952226838146558 1107 92-93 0.3513581362328644 1107 94-95 0.12712505209453084 1107 96-97 0.17312991351423435 1107 98-99 0.15683345360847412 1107 100 0.004575010965158555 1108 1 0.37302111475201016 1108 2 3.594800586082176 1108 3 1.914594186696064 1108 4 0.9028036795487964 1108 5 0.21553884786870015 1108 6 0.09716794252076255 1108 7 0.1054772342353516 1108 8 0.16513862517309974 1108 9 0.019714386525464533 1108 10-11 0.10084912278892944 1108 12-13 0.06016176423094777 1108 14-15 -0.047572314330224685 1108 16-17 -0.17180504019317766 1108 18-19 -0.03248362455114062 1108 20-21 0.10436299633780521 1108 22-23 -0.020535633445618373 1108 24-25 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76-77 0.29595438989001366 2210 78-79 0.4491117529912785 2210 80-81 0.5071628736208318 2210 82-83 0.5524401604625027 2210 84-85 0.9007010638498336 2210 86-87 0.8239970422218263 2210 88-89 0.6926972415330255 2210 90-91 0.6881061710862255 2210 92-93 0.6638267128028801 2210 94-95 0.6726005710935787 2210 96-97 0.4306355297572999 2210 98-99 0.5866355247716761 2210 100 0.5976495574999667 2211 1 0.39856016419105345 2211 2 3.9398798786918583 2211 3 2.2000283174665043 2211 4 0.9724562163247512 2211 5 0.6124218535037684 2211 6 0.41176823026209064 2211 7 0.3041792839026769 2211 8 0.36368303035089866 2211 9 0.3045564675226231 2211 10-11 0.17972402273604615 2211 12-13 0.13316223973296104 2211 14-15 0.09681366359377108 2211 16-17 0.14804503379698986 2211 18-19 0.09983644962846228 2211 20-21 -0.07089433180320981 2211 22-23 -0.1542510700518278 2211 24-25 -0.043089605395643105 2211 26-27 0.07638123078899639 2211 28-29 -0.15139145747062344 2211 30-31 0.02315084498675901 2211 32-33 0.023795693438920296 2211 34-35 0.14164444802574394 2211 36-37 0.02428792476548125 2211 38-39 0.062417915154313164 2211 40-41 -0.10819107602114286 2211 42-43 0.17682007974985225 2211 44-45 0.22918080401554874 2211 46-47 0.3817687943971606 2211 48-49 0.2396248950746056 2211 50-51 0.09768431202656558 2211 52-53 0.09929114856272037 2211 54-55 0.048910736642326924 2211 56-57 -0.08122061514970369 2211 58-59 0.0845653864121445 2211 60-61 0.21212898258187352 2211 62-63 0.4887208558836704 2211 64-65 0.2570490603065565 2211 66-67 0.28585816830982935 2211 68-69 0.10106618772677223 2211 70-71 0.1613012172991901 2211 72-73 0.14094732721627778 2211 74-75 -6.489138638521297E-4 2211 76-77 -0.1262045352688972 2211 78-79 0.12171611604717825 2211 80-81 0.014495943417241364 2211 82-83 -0.09505722003066097 2211 84-85 0.0030723206774858625 2211 86-87 0.1379483120905718 2211 88-89 0.13116904315612032 2211 90-91 0.14334569383355245 2211 92-93 0.33980954247230244 2211 94-95 0.39106412132547064 2211 96-97 0.23504944857937105 2211 98-99 0.39337169671933836 2211 100 -0.1371824244319626 2212 1 0.6535652798969984 2212 2 4.125493371378386 2212 3 2.3144652558641106 2212 4 1.3217868187003319 2212 5 0.6396062405821823 2212 6 0.5650855017952452 2212 7 0.5206370471437438 2212 8 0.20072383887443124 2212 9 0.27624923864777884 2212 10-11 0.2739798157769471 2212 12-13 0.28354608707294204 2212 14-15 0.26433488385177384 2212 16-17 0.5683760204555028 2212 18-19 0.5616356293731641 2212 20-21 0.3028072613737791 2212 22-23 0.32522173636540685 2212 24-25 0.12067057882784837 2212 26-27 0.09678307263673247 2212 28-29 0.4377210425656699 2212 30-31 0.40871197065501264 2212 32-33 0.38133707645090453 2212 34-35 0.5662296499388333 2212 36-37 0.5345720908570186 2212 38-39 0.3556775732877071 2212 40-41 0.166964694078942 2212 42-43 -0.015058095787104264 2212 44-45 0.0655764624896662 2212 46-47 -0.011974556879053466 2212 48-49 0.009483465406610492 2212 50-51 0.27432765391350244 2212 52-53 0.00400156669545737 2212 54-55 0.06994320903348239 2212 56-57 0.3209343699290841 2212 58-59 0.35707618650467765 2212 60-61 0.037818925307263385 2212 62-63 0.06578168196612211 2212 64-65 0.07621732492552269 2212 66-67 0.18511022015801615 2212 68-69 0.05152756922873181 2212 70-71 -0.020292792146229033 2212 72-73 -0.06236205538528594 2212 74-75 0.07111955228585387 2212 76-77 0.06078734251945406 2212 78-79 0.06030780140367753 2212 80-81 0.1675881626075757 2212 82-83 0.2686109105248633 2212 84-85 0.5536374682489047 2212 86-87 0.3319792463820619 2212 88-89 0.20835280105594123 2212 90-91 0.1140240354736548 2212 92-93 0.33431439343925007 2212 94-95 0.4533668375378568 2212 96-97 0.214151039626838 2212 98-99 0.3239253835606952 2212 100 0.244686390863464 2213 1 -0.7731238879006916 2213 2 -25.654481906742753 2213 3 -14.679819530269612 2213 4 -6.504502063887259 2213 5 -2.2122620782474627 2213 6 -1.3724917540762007 2213 7 -1.4353907690994703 2213 8 -1.3075833180819671 2213 9 -1.144340672733371 2213 10-11 -1.1319827545276198 2213 12-13 -1.4262960012375743 2213 14-15 -1.476757118427706 2213 16-17 -1.4487771918745977 2213 18-19 -1.485233391469741 2213 20-21 -1.4813422426744935 2213 22-23 -1.5916674639176023 2213 24-25 -1.6556755660181324 2213 26-27 -1.713687799318805 2213 28-29 -1.5857984665205862 2213 30-31 -1.6290873141173492 2213 32-33 -1.4347836257826003 2213 34-35 -1.4798637150722698 2213 36-37 -1.7591440645329328 2213 38-39 -1.6342272006777847 2213 40-41 -1.6625300490805586 2213 42-43 -1.7478890537461425 2213 44-45 -1.796634851761759 2213 46-47 -2.0326791304266436 2213 48-49 -2.039755194326183 2213 50-51 -1.8706979626881477 2213 52-53 -2.0085644707287322 2213 54-55 -1.9911181799617026 2213 56-57 -1.6539638381476465 2213 58-59 -1.7826423474713877 2213 60-61 -1.8244715374984253 2213 62-63 -1.7206982308190533 2213 64-65 -1.5927001430915766 2213 66-67 -1.6673912871057581 2213 68-69 -1.7233753189364265 2213 70-71 -1.7294679678560172 2213 72-73 -1.8333935468744116 2213 74-75 -2.0199831866670728 2213 76-77 -1.8352403636040293 2213 78-79 -1.8608733152654189 2213 80-81 -1.8904917347608396 2213 82-83 -2.15240296180783 2213 84-85 -1.8199333841603789 2213 86-87 -1.5450080705783016 2213 88-89 -1.4670135535130626 2213 90-91 -1.3680622378269902 2213 92-93 -1.8256861296294566 2213 94-95 -1.9646380132361507 2213 96-97 -1.9401872646945364 2213 98-99 -1.8704517654946144 2213 100 -1.8917767286632419 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 10.0 5 19.0 6 17.0 7 15.0 8 16.0 9 35.0 10 206.0 11 972.0 12 2659.0 13 4913.0 14 6491.0 15 7635.0 16 8353.0 17 8825.0 18 9011.0 19 9356.0 20 10548.0 21 12463.0 22 14051.0 23 14654.0 24 14245.0 25 14247.0 26 14653.0 27 16405.0 28 19004.0 29 22388.0 30 27086.0 31 33372.0 32 42434.0 33 56459.0 34 75669.0 35 107616.0 36 163771.0 37 246749.0 38 142195.0 39 298.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.46073561498319 18.398839968650755 10.033124247331354 31.1073001690347 2 13.668400056238719 21.28128637463978 33.03938718924042 32.01092637988108 3 17.2328711182425 25.869589571791508 26.89466627636776 30.00287303359823 4 11.941932924884508 18.495485589394185 31.67396857034177 37.88861291537953 5 12.482466539369971 37.549025818303576 33.377193379365266 16.591314262961188 6 33.675958928318 33.819957496467836 16.749935141887853 15.754148433326312 7 27.344241433911513 31.457735327929896 21.59055579254846 19.607467445610126 8 28.147844855636784 32.783791405453485 19.443661106170055 19.624702632739684 9 27.340473608098335 14.595173535791758 19.67281272595806 38.39154013015184 10-11 24.566172529517623 26.096352676719338 26.309933449207456 23.027541344555587 12-13 26.805532582965697 23.55568691357757 26.464965821305487 23.173814682151246 14-15 25.50869701976579 23.99182541605053 24.094911583613232 26.40456598057045 16-17 22.870079780924428 26.787049777783807 27.349470273641113 22.993400167650655 18-19 24.273211218689802 25.886861477133216 28.26818288006138 21.5717444241156 20-21 23.43275078133719 25.553578020534136 28.23608243835942 22.77758875976926 22-23 22.72081143044075 25.229556773110883 26.605361159382575 25.444270637065795 24-25 23.19382632936786 26.061845143159218 26.09026463106625 24.654063896406676 26-27 24.038834267188218 24.41535804999173 27.14567503657259 24.400132646247457 28-29 23.23939525649911 24.08225357407168 28.581504015267683 24.09684715416152 30-31 24.089729385845217 25.234548533608532 27.32668892114 23.34903315940625 32-33 22.212610213103513 25.1763816221598 27.622550104668004 24.988458060068684 34-35 21.818233563920835 25.771529995423787 28.133587456931426 24.276648983723952 36-37 24.661346266609684 24.724771844103184 27.058778885968582 23.55510300331855 38-39 24.51057678190258 24.71662356143343 26.760963599606967 24.011836057057025 40-41 22.961943324959215 25.255390959478525 27.15897011475144 24.62369560081082 42-43 24.642565433294543 24.70536043427035 26.947864789261782 23.704209343173325 44-45 25.31716365653713 24.230435965062078 26.07902504872317 24.37337532967763 46-47 23.792972736813997 24.823943423343156 28.213645282650607 23.16943855719224 48-49 24.919153512309407 25.174265581333348 26.710721620137857 23.195859286219385 50-51 23.37191323858846 25.473942750047218 26.708782862425345 24.445361148938975 52-53 22.899780305981082 25.544784232638882 25.15740520668499 26.398030254695044 54-55 23.437645436447152 26.211861197939967 24.68355288306435 25.66694048254853 56-57 24.38467467432192 24.526132407329598 25.512089643812047 25.577103274536434 58-59 23.710581006596186 23.95780247582904 26.915379054847744 25.416237462727025 60-61 24.21943543643397 25.0994144015122 26.17081296177662 24.510337200277213 62-63 22.464761433163062 25.22059401574632 26.407441778089424 25.907202773001192 64-65 21.998411406068286 25.636273436956408 26.905838308608317 25.45947684836699 66-67 24.27142442111332 24.782436020760354 26.035024400690926 24.911115157435397 68-69 24.598943287917216 24.591803952029085 25.45200355520853 25.357249204845168 70-71 23.220015408285683 24.984015598065152 26.064131905769973 25.73183708787919 72-73 24.546426013659296 24.558354739522265 25.872276782587676 25.02294246423077 74-75 25.10983298337696 24.169717771141634 25.283252294371827 25.43719695110958 76-77 23.598403829940658 24.66974623390072 27.366536273018816 24.365313663139805 78-79 24.532470342989868 24.902852593918194 26.39391449040363 24.170762572688307 80-81 23.037540965979375 25.18114485172733 26.604280611650193 25.177033570643104 82-83 22.62649106670112 25.0697081024036 25.30304727862821 27.00075355226707 84-85 23.038625934678766 25.444586554721315 24.83052558865957 26.686261921940346 86-87 23.904079728755978 24.172658295328 25.64502903765747 26.278232938258554 88-89 23.435013873742168 23.73417349812562 26.68741478558721 26.143397842544996 90-91 23.697232076988094 24.494146698248098 26.20060111938495 25.60802010537886 92-93 22.043232743043006 24.882363570654135 26.123554391037224 26.950849295265634 94-95 21.558121349642423 25.133388114578032 26.917136515976082 26.391354019803465 96-97 23.728062448332405 24.350648470639253 26.00157205003456 25.919717030993777 98-99 23.450471930374572 24.16317849873107 25.7699643217565 26.61638524913786 100 22.27739428670661 24.556452779948483 27.089574107840587 26.076578825504328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 69.0 1 53.5 2 37.0 3 32.5 4 28.0 5 61.5 6 169.0 7 359.5 8 556.0 9 617.5 10 478.0 11 333.5 12 312.5 13 350.0 14 397.5 15 428.0 16 514.0 17 618.5 18 667.0 19 716.5 20 802.5 21 917.0 22 1128.5 23 1425.0 24 1761.0 25 2153.5 26 2806.0 27 3780.0 28 4839.0 29 5859.0 30 6823.5 31 7835.0 32 9360.0 33 11041.5 34 12458.0 35 14296.0 36 16435.0 37 18651.5 38 20901.5 39 22732.0 40 24606.0 41 26243.0 42 27554.0 43 29636.0 44 31410.5 45 32558.0 46 35501.5 47 39928.0 48 42739.0 49 50138.5 50 79899.0 51 81239.0 52 50034.5 53 39934.5 54 37210.5 55 34060.5 56 32101.0 57 29825.5 58 28257.5 59 27688.0 60 25851.5 61 22809.0 62 20057.5 63 16970.5 64 13817.0 65 11652.0 66 9655.0 67 7611.5 68 5891.5 69 4535.0 70 3622.0 71 2965.0 72 2368.0 73 1792.5 74 1360.0 75 975.0 76 603.0 77 418.0 78 282.0 79 137.0 80 70.5 81 30.5 82 11.5 83 6.5 84 5.5 85 3.5 86 2.5 87 2.0 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7456335310125004 2 5.538359515884804 3 0.0 4 0.004969083267380046 5 0.16388940085504372 6 0.0526722826342285 7 0.035054623777153786 8 0.04228238125697931 9 0.04011405401303165 10-11 0.04255342216247276 12-13 0.040927176729512016 14-15 0.044089320626935685 16-17 0.03401563363942886 18-19 0.028504468561061903 20-21 0.022360874703210208 22-23 0.02303847696694385 24-25 0.018882516416044177 26-27 0.012738922558192483 28-29 0.01743696492007907 30-31 0.022586742124454755 32-33 9.938166534760094E-4 34-35 0.003026623444676937 36-37 0.0031621438974236663 38-39 0.005104603720126775 40-41 0.0054659915941180515 42-43 0.0061435938578516935 44-45 0.007318104448323341 46-47 0.015223464191882507 48-49 0.024529201947157863 50-51 0.020147373975013642 52-53 0.0275558253918348 54-55 0.023580558777930766 56-57 0.01382308618016631 58-59 0.01300996346368594 60-61 0.01020920744025355 62-63 0.015630025550122693 64-65 0.0185211285420529 66-67 0.01946977171128 68-69 0.026968570096598976 70-71 0.02678787615960334 72-73 0.024529201947157863 74-75 0.025071283758144776 76-77 0.01761765885707471 78-79 0.013506871790423943 80-81 0.012016146810209929 82-83 0.0077698392908124355 84-85 0.01025438092450246 86-87 0.008266747617550441 88-89 0.0069567165743320645 90-91 0.006821196121585336 92-93 0.003975266613904038 94-95 0.0015810719487118331 96-97 8.131227164803712E-4 98-99 6.324287794847333E-4 100 0.10950052581935665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1106844.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.58978211701473 #Duplication Level Percentage of deduplicated Percentage of total 1 79.3287972375818 42.51212959566848 2 11.040274865311751 11.832918490880218 3 3.877686153815511 6.2341306830341425 4 1.8168527841650282 3.8945897936838176 5 1.002709187524988 2.686748344309649 6 0.6332132852850719 2.0360257195215654 7 0.4188403374918477 1.5711893699603523 8 0.30106684396435995 1.2907285256565857 9 0.21939339662984084 1.0581519890974467 >10 1.2602226456383752 12.151479874079437 >50 0.07186786719311161 2.645965640372369 >100 0.02231367687932019 2.1962583611528017 >500 0.0015213866667771634 0.6005194894917443 >1k 0.004564160000331491 5.265076592134587 >5k 3.380859259504808E-4 1.0097134152775655 >10k+ 3.380859259504808E-4 3.0143741156792565 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 19784 1.7874244247608515 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 13491 1.2188709520040764 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 5616 0.5073885750837517 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5530 0.4996187357929392 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4836 0.4369179396554528 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4784 0.4322198972935662 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 4328 0.3910216796585608 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3780 0.3415115409217559 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3389 0.30618587623910865 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2661 0.24041328317269645 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2598 0.23472142415733385 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2528 0.2283971363624865 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 2012 0.18177810061761188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1936 0.17491173101177765 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1867 0.1686777901854281 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1787 0.1614500327056026 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1773 0.16018517514663314 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1747 0.15783615396568984 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 1690 0.15268637676131416 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1665 0.15042770254886867 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 1615 0.1459103541239777 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 1587 0.1433806390060388 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 1587 0.1433806390060388 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1575 0.14229647538406498 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 1476 0.1333521255027809 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 1319 0.11916765144862329 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1194 0.1078742803863959 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1162 0.10498317739446571 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.034696849781903E-5 0.0 14-15 0.0 0.0 0.0 9.034696849781903E-5 0.0 16-17 0.0 0.0 0.0 9.034696849781903E-5 0.0 18-19 0.0 4.5173484248909515E-5 0.0 9.034696849781903E-5 0.0 20-21 0.0 9.034696849781903E-5 0.0 9.034696849781903E-5 0.0 22-23 0.0 9.034696849781903E-5 0.0 2.2586742124454758E-4 0.0 24-25 0.0 9.034696849781903E-5 0.0 5.420818109869142E-4 0.0 26-27 0.0 9.034696849781903E-5 0.0 0.001310031043218376 0.0 28-29 0.0 9.034696849781903E-5 0.0 0.0036138787399127613 0.0 30-31 0.0 9.034696849781903E-5 0.0 0.01115785060948065 0.0 32-33 0.0 9.034696849781903E-5 0.0 0.03261525562771267 0.0 34-35 0.0 9.034696849781903E-5 0.0 0.06527568473967424 0.0 36-37 0.0 9.034696849781903E-5 0.0 0.11582481361420399 0.0 38-39 0.0 9.034696849781903E-5 0.0 0.1976339935889791 0.0 40-41 0.0 9.034696849781903E-5 0.0 0.32159003436798683 0.0 42-43 0.0 9.034696849781903E-5 0.0 0.4155508816057186 0.0 44-45 0.0 9.034696849781903E-5 0.0 0.518185037819241 0.0 46-47 0.0 9.034696849781903E-5 0.0 0.6492333156253275 0.0 48-49 0.0 9.034696849781903E-5 0.0 0.7744090404790558 0.0 50-51 0.0 9.034696849781903E-5 0.0 0.9100650136785311 0.0 52-53 0.0 9.034696849781903E-5 0.0 1.0679011676442207 0.0 54-55 0.0 9.034696849781903E-5 0.0 1.2272280465901249 0.0 56-57 0.0 9.034696849781903E-5 0.0 1.4020946041176534 0.0 58-59 0.0 9.034696849781903E-5 0.0 1.6041104256787766 0.0 60-61 0.0 9.034696849781903E-5 0.0 1.8007506026142799 0.0 62-63 0.0 9.034696849781903E-5 0.0 2.0051154453563464 0.0 64-65 0.0 9.034696849781903E-5 0.0 2.2413727679781434 0.0 66-67 0.0 9.034696849781903E-5 0.0 2.502656200873836 0.0 68-69 0.0 9.034696849781903E-5 0.0 2.807170658195735 0.0 70-71 0.0 9.034696849781903E-5 0.0 3.2323434919464713 0.0 72-73 0.0 9.034696849781903E-5 0.0 3.6041664407992453 0.0 74-75 0.0 9.034696849781903E-5 0.0 3.968580938235198 0.0 76-77 0.0 9.034696849781903E-5 0.0 4.3778527055303185 0.0 78-79 0.0 9.034696849781903E-5 0.0 4.807362193768951 0.0 80-81 0.0 9.034696849781903E-5 0.0 5.280509267792029 0.0 82-83 0.0 9.034696849781903E-5 0.0 5.857600529071847 0.0 84-85 0.0 9.034696849781903E-5 0.0 6.424076021553173 0.0 86-87 0.0 9.034696849781903E-5 0.0 6.993578137479175 0.0 88 0.0 9.034696849781903E-5 0.0 7.4790123992179565 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTAGC 105 4.991513E-5 28.467358 1 CTATAAG 175 2.6776925E-6 22.773884 1 TGTACTA 150 3.2750337E-5 21.947351 5 GTACATA 245 1.0625263E-7 20.333826 1 CCTATAC 335 4.0017767E-11 19.653456 3 CGAGCGT 60 0.0051039825 19.557774 60-61 GTCTTAG 270 2.9553848E-7 18.451065 1 ACCGTCC 155 0.0010398627 18.176374 8 GTCTACG 165 0.0010634514 18.115591 1 GATCTAC 165 0.0010634514 18.115591 1 GAGTACT 3935 0.0 17.778288 12-13 TAGACTG 265 4.4459193E-7 17.747185 5 GGCTATA 170 0.0012992058 17.582779 1 AAAGTAC 565 0.0 17.4794 3 GTACTTT 4090 0.0 17.33374 14-15 AGTACTT 3930 0.0 17.203562 12-13 GTAGACT 305 1.0387339E-7 16.961655 4 TAGACTA 310 1.2502824E-7 16.68808 5 GATATAA 270 6.0266466E-6 16.605959 1 TAGCACA 400 4.965841E-10 16.460514 4 >>END_MODULE