FastQCFastQC Report
Wed 25 May 2016
SRR1295124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712670
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41270.11115989301499998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15800.017.1640381
ATACCGA2454.428083E-516.4538566
GTATATA27400.015.7460391
GTACTAA9400.015.7364671
GTACATG157950.015.0623081
TACATGG157850.014.6781432
GTATAGC8400.014.6748791
TATACTG17100.014.391695
TACCGAT2851.6614574E-414.0908297
CATGGGG41350.014.0106214
ACATGGG159850.013.9051473
GTATAAT17950.013.7347071
CTACACT15400.013.3838354
GTATAGA14750.013.37155151
ATAGGAC9650.013.2774873
TACTGCG3809.601328E-613.2101547
GTATTAT21000.013.2073921
GTATAGG9950.013.00828551
TCTATAC13350.012.9358743
GTAGGAC7351.07320375E-1012.8847763
Produced by FastQC (version 0.11.5)