##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295124_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3712670
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.13009424484266	33.0	27.0	33.0	6.0	33.0
2	22.015468113244648	27.0	2.0	33.0	2.0	33.0
3	24.5802508706672	27.0	15.0	33.0	6.0	33.0
4	24.76942443039645	33.0	6.0	37.0	2.0	37.0
5	26.832528880832392	33.0	15.0	37.0	2.0	37.0
6	27.665836715894493	33.0	15.0	37.0	2.0	37.0
7	27.338333059496264	33.0	15.0	37.0	6.0	37.0
8	27.972380523989475	33.0	22.0	37.0	2.0	37.0
9	29.09775067538995	33.0	27.0	37.0	15.0	37.0
10-11	28.63198641409013	35.0	24.5	37.0	8.5	37.0
12-13	27.470773594205788	33.0	15.0	37.0	4.0	37.0
14-15	29.267783024077012	33.0	22.0	37.0	4.0	40.0
16-17	29.64414639598995	37.0	27.0	40.0	4.0	40.0
18-19	29.802318143007593	37.0	27.0	40.0	6.0	40.0
20-21	29.30815194994438	33.0	24.5	40.0	4.0	40.0
22-23	30.11031966212995	37.0	27.0	40.0	10.5	40.0
24-25	30.308024683044817	37.0	27.0	40.0	10.5	40.0
26-27	28.742065009817733	33.0	18.5	40.0	2.0	40.0
28-29	29.48097231911266	33.0	22.0	40.0	6.0	40.0
30-31	30.249926468013587	37.0	27.0	40.0	15.0	40.0
32-33	28.967867329980848	33.0	22.0	40.0	4.0	40.0
34-35	28.68414321768431	33.0	22.0	37.0	6.0	40.0
36-37	28.96671788766575	33.0	22.0	37.0	6.0	40.0
38-39	29.12821621636181	33.0	24.5	37.0	6.0	40.0
40-41	28.202767280690175	33.0	22.0	37.0	4.0	40.0
42-43	28.889758044749463	33.0	22.0	37.0	6.0	40.0
44-45	28.722001282096173	33.0	22.0	37.0	6.0	40.0
46-47	28.408066835996735	33.0	22.0	37.0	6.0	40.0
48-49	28.425712088604698	33.0	22.0	37.0	6.0	40.0
50-51	28.20658609572087	33.0	22.0	37.0	6.0	40.0
52-53	28.500635526454005	33.0	22.0	37.0	6.0	40.0
54-55	28.521832939636433	33.0	22.0	37.0	6.0	40.0
56-57	28.499003951334217	33.0	22.0	37.0	6.0	40.0
58-59	28.403234330010477	33.0	22.0	37.0	6.0	40.0
60-61	28.3574280773675	33.0	22.0	37.0	6.0	40.0
62-63	28.367562024095868	33.0	22.0	37.0	6.0	40.0
64-65	27.91741563349288	33.0	22.0	37.0	6.0	40.0
66-67	28.153943388450898	33.0	22.0	37.0	10.5	40.0
68-69	27.61668610460935	33.0	22.0	37.0	6.0	38.5
70-71	27.828581048140556	33.0	22.0	37.0	10.5	37.0
72-73	27.91031225506172	33.0	22.0	37.0	10.5	37.0
74-75	28.02709115003488	33.0	22.0	37.0	15.0	37.0
76-77	27.817777906466233	33.0	22.0	37.0	10.5	37.0
78-79	27.29533893397474	33.0	22.0	37.0	10.5	37.0
80-81	27.15161339413414	27.0	22.0	35.0	6.0	37.0
82-83	27.25458847675662	27.0	22.0	33.0	15.0	37.0
84-85	27.174168724933807	27.0	22.0	33.0	10.5	37.0
86-87	27.02747887638815	27.0	22.0	33.0	15.0	37.0
88-89	26.95191667990961	27.0	22.0	33.0	15.0	37.0
90-91	27.13184541044585	27.0	22.0	33.0	15.0	37.0
92-93	27.03823528619565	27.0	22.0	33.0	15.0	37.0
94-95	26.88487395863354	27.0	22.0	33.0	15.0	37.0
96-97	26.96906781372974	27.0	22.0	33.0	15.0	37.0
98-99	27.039076325124505	27.0	22.0	33.0	15.0	37.0
100	26.648172608931038	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.183810541885396
1101	2	11.205234074756127
1101	3	8.47443402077067
1101	4	12.845692996710273
1101	5	10.87596608464482
1101	6	10.07168041248974
1101	7	9.498760806620211
1101	8	9.121961643647627
1101	9	7.358916288506986
1101	10-11	7.824262159137113
1101	12-13	9.135382322920844
1101	14-15	8.026724734116094
1101	16-17	8.297670644694964
1101	18-19	8.127325614706894
1101	20-21	7.387351183609788
1101	22-23	7.464879122237601
1101	24-25	7.347052784712712
1101	26-27	8.720496484608343
1101	28-29	8.416367600280974
1101	30-31	5.603062871244646
1101	32-33	7.074878389488088
1101	34-35	8.812750092561544
1101	36-37	8.298088593157903
1101	38-39	8.170536730537858
1101	40-41	9.163803029053238
1101	42-43	8.625132266338667
1101	44-45	8.56312218434342
1101	46-47	7.452573739491882
1101	48-49	5.664610922575257
1101	50-51	7.0149666518591545
1101	52-53	6.029866390413304
1101	54-55	4.2688533388505085
1101	56-57	6.275550920891934
1101	58-59	6.2448305042463375
1101	60-61	6.766022892544989
1101	62-63	6.2393747831222015
1101	64-65	6.795920446694662
1101	66-67	5.8323108595112245
1101	68-69	6.821186953262849
1101	70-71	6.391870409114187
1101	72-73	5.687929731729575
1101	74-75	5.027083823522698
1101	76-77	5.111101588721304
1101	78-79	5.126881707922006
1101	80-81	5.081954701399521
1101	82-83	4.581924626011688
1101	84-85	4.346565724021854
1101	86-87	4.1217432775087595
1101	88-89	4.0390323173664235
1101	90-91	3.838767198814754
1101	92-93	3.864048459778214
1101	94-95	3.6910882178668274
1101	96-97	3.738089410646463
1101	98-99	3.5271029276798522
1101	100	3.8238424601347774
1102	1	6.643780072551952
1102	2	10.519564367681298
1102	3	8.154621303257834
1102	4	12.584310372810943
1102	5	10.585421675729986
1102	6	9.50296147846943
1102	7	6.6749720025913
1102	8	7.804368287065078
1102	9	6.522687585474831
1102	10-11	7.590421256430496
1102	12-13	9.191834242612838
1102	14-15	6.38639035446996
1102	16-17	7.527571414633716
1102	18-19	7.469726445819223
1102	20-21	7.070064176126387
1102	22-23	6.653529140557044
1102	24-25	6.959213821942466
1102	26-27	8.618308004097923
1102	28-29	7.874047004427521
1102	30-31	4.2978375469513
1102	32-33	4.489721977633003
1102	34-35	5.158541163112126
1102	36-37	5.348101537486038
1102	38-39	4.683340881466602
1102	40-41	6.441889961758065
1102	42-43	7.049345916756657
1102	44-45	6.601641111773297
1102	46-47	5.562103464791548
1102	48-49	3.963388584857327
1102	50-51	5.251147451905091
1102	52-53	6.1213586634568955
1102	54-55	5.620630757417064
1102	56-57	6.487669993050321
1102	58-59	6.117833448081825
1102	60-61	6.151933786628582
1102	62-63	4.244162740835112
1102	64-65	6.175927993066676
1102	66-67	4.003337423850006
1102	68-69	6.271686145934805
1102	70-71	5.609381564727027
1102	72-73	5.26140911166771
1102	74-75	4.363962956613225
1102	76-77	3.532099254664004
1102	78-79	4.5430997357577745
1102	80-81	4.571013506005908
1102	82-83	4.0587616194822616
1102	84-85	3.2161449216138003
1102	86-87	3.349486064002548
1102	88-89	3.502939721456862
1102	90-91	3.3931142745058764
1102	92-93	3.3082544904159015
1102	94-95	3.2483613805471023
1102	96-97	3.412486255525618
1102	98-99	3.2215024870343676
1102	100	3.3978870349523405
1103	1	6.705369984560388
1103	2	10.899547953393025
1103	3	8.071956477535494
1103	4	12.005119795867007
1103	5	10.431484533567346
1103	6	9.215447707325907
1103	7	8.226369438773567
1103	8	8.243318308432404
1103	9	7.312938334638108
1103	10-11	7.62867886535485
1103	12-13	8.803681640699832
1103	14-15	8.751739772324683
1103	16-17	8.243953651916886
1103	18-19	7.3783867144855755
1103	20-21	8.570472311654044
1103	22-23	6.931514913969291
1103	24-25	5.343422714670648
1103	26-27	6.111009265358238
1103	28-29	7.147996182863647
1103	30-31	6.242263027258723
1103	32-33	8.082394226377428
1103	34-35	7.302657764674883
1103	36-37	4.3508217875686945
1103	38-39	6.508435858250056
1103	40-41	7.003020802977272
1103	42-43	7.689260081529454
1103	44-45	5.929138899895676
1103	46-47	6.627426810588069
1103	48-49	5.849865975351417
1103	50-51	6.732806608424994
1103	52-53	6.354538722710387
1103	54-55	5.104755455039996
1103	56-57	6.065316960392945
1103	58-59	5.675105853389283
1103	60-61	5.180732269875158
1103	62-63	2.961266239718391
1103	64-65	5.201322439130184
1103	66-67	4.265662156235685
1103	68-69	5.411463024005133
1103	70-71	3.992644348856924
1103	72-73	4.63877216148429
1103	74-75	3.406980839823472
1103	76-77	3.7910172707829375
1103	78-79	3.084547675934491
1103	80-81	4.305674804485886
1103	82-83	3.683994242357457
1103	84-85	3.4962154468579563
1103	86-87	2.947711693815915
1103	88-89	3.1844806062340503
1103	90-91	2.802029546197634
1103	92-93	2.5283614708010838
1103	94-95	2.927168129746967
1103	96-97	3.0436935617135177
1103	98-99	2.831104420940722
1103	100	2.9422925201474612
1104	1	6.42081326525453
1104	2	9.941769449478102
1104	3	7.65594107258039
1104	4	12.262838284449092
1104	5	9.679938004631996
1104	6	8.418539242196083
1104	7	4.256259625879391
1104	8	6.787971073098163
1104	9	6.881791024494607
1104	10-11	7.401343391229496
1104	12-13	7.711071989380397
1104	14-15	7.65957390643738
1104	16-17	7.596647722700094
1104	18-19	7.13545804174213
1104	20-21	7.850220230008453
1104	22-23	6.810041872412921
1104	24-25	6.164111344241572
1104	26-27	8.581542459382586
1104	28-29	7.543192642447149
1104	30-31	6.5818275236301105
1104	32-33	7.898803452259042
1104	34-35	8.219942377416523
1104	36-37	6.926103028491308
1104	38-39	7.524494043899519
1104	40-41	7.515590747915592
1104	42-43	7.585769470079235
1104	44-45	7.079036240548
1104	46-47	6.870430201477149
1104	48-49	5.819859024871651
1104	50-51	6.080210058489811
1104	52-53	6.338438312847515
1104	54-55	5.17486066638984
1104	56-57	6.329910380757301
1104	58-59	4.037846504557923
1104	60-61	4.621905292367991
1104	62-63	4.068318451365737
1104	64-65	2.998791632051205
1104	66-67	3.5475725238144626
1104	68-69	4.581302364371478
1104	70-71	2.844727592290919
1104	72-73	2.865280220012032
1104	74-75	3.5146533731662863
1104	76-77	4.042991452083914
1104	78-79	4.502302205933599
1104	80-81	3.3501385852878407
1104	82-83	3.2073167418851014
1104	84-85	3.197638588343338
1104	86-87	0.9217494243316438
1104	88-89	0.6355767012541804
1104	90-91	2.454247428736153
1104	92-93	2.7106064315079976
1104	94-95	2.6955763247394593
1104	96-97	3.0558806412408934
1104	98-99	2.735487681550783
1104	100	1.1012972737965967
1105	1	5.755249643746268
1105	2	-2.897744657569273
1105	3	0.44529953623509044
1105	4	8.444270272797095
1105	5	8.706954329172223
1105	6	8.1857508635822
1105	7	5.374779439619111
1105	8	6.028784147248771
1105	9	5.087637927203815
1105	10-11	5.159498687793082
1105	12-13	5.141211662503583
1105	14-15	6.073462831734361
1105	16-17	6.219167113369782
1105	18-19	6.275268427439219
1105	20-21	7.2091062825869585
1105	22-23	2.1404950936240574
1105	24-25	4.115901573330735
1105	26-27	7.040853247961206
1105	28-29	6.1296524426565036
1105	30-31	5.372419933536769
1105	32-33	7.217843168750274
1105	34-35	6.17424855769093
1105	36-37	6.357947032412238
1105	38-39	4.177784961725759
1105	40-41	5.575708269395449
1105	42-43	5.711151472981669
1105	44-45	6.276705869809128
1105	46-47	5.478005430717303
1105	48-49	5.2153118286300035
1105	50-51	6.18568657327809
1105	52-53	5.793402215304617
1105	54-55	5.399100264986064
1105	56-57	5.291037941124355
1105	58-59	1.9614640240933454
1105	60-61	4.476140647301037
1105	62-63	-0.7844514018507596
1105	64-65	-0.4453891697970924
1105	66-67	0.964512551237199
1105	68-69	3.105036799492087
1105	70-71	3.249241617960209
1105	72-73	2.8851639112817153
1105	74-75	2.7996790418313005
1105	76-77	1.8274821858564394
1105	78-79	3.2444546817742683
1105	80-81	1.722439275350009
1105	82-83	1.060318270592866
1105	84-85	1.413500084856107
1105	86-87	-0.39792218460146955
1105	88-89	1.558092447913971
1105	90-91	1.32122138289931
1105	92-93	1.7358623007764784
1105	94-95	1.1259872341465424
1105	96-97	2.073092490677702
1105	98-99	1.9709133343146625
1105	100	2.094225350019105
1106	1	6.066386823171321
1106	2	10.378430880111381
1106	3	6.912057286616925
1106	4	11.833265382657476
1106	5	9.929763395611847
1106	6	9.43457223358665
1106	7	6.734080304350687
1106	8	7.597578640944409
1106	9	7.004757708793694
1106	10-11	5.84460135102151
1106	12-13	8.080857101634518
1106	14-15	8.050839174099856
1106	16-17	7.958911007904337
1106	18-19	7.3400938631466595
1106	20-21	8.425221042864528
1106	22-23	6.300473579134515
1106	24-25	5.1719133966517425
1106	26-27	7.874711055245978
1106	28-29	6.407664831646198
1106	30-31	5.534418641106335
1106	32-33	7.454106921692034
1106	34-35	6.969622357040727
1106	36-37	6.189223447855017
1106	38-39	7.173636381442488
1106	40-41	6.9363038953173834
1106	42-43	6.280590338973155
1106	44-45	4.66461468549209
1106	46-47	6.137389844864831
1106	48-49	5.983735385467508
1106	50-51	6.743765287950488
1106	52-53	5.875052066391099
1106	54-55	5.392352730897155
1106	56-57	5.043604833505615
1106	58-59	5.201375570336435
1106	60-61	5.011122994817391
1106	62-63	5.022234253418084
1106	64-65	5.309737975025474
1106	66-67	3.1762474987101257
1106	68-69	3.6183798265957243
1106	70-71	3.1644329441714056
1106	72-73	3.0198122996300683
1106	74-75	3.0573329954792072
1106	76-77	3.6859633838772297
1106	78-79	4.26113963131235
1106	80-81	3.7727821618495305
1106	82-83	2.296890929802636
1106	84-85	2.949341288279907
1106	86-87	3.4467712458473514
1106	88-89	3.4354615920425147
1106	90-91	3.1789010182088724
1106	92-93	3.1061752755660628
1106	94-95	1.5780463798047109
1106	96-97	1.6274668777028367
1106	98-99	2.057258896737089
1106	100	2.9176176904662263
1107	1	6.2055939557858935
1107	2	10.583723639740406
1107	3	6.364160951651055
1107	4	11.587347098457112
1107	5	9.757259805816819
1107	6	8.458657231577845
1107	7	4.146171149581921
1107	8	6.723076286785169
1107	9	5.945982219725938
1107	10-11	4.844532378219096
1107	12-13	6.407311467694505
1107	14-15	6.602481200033402
1107	16-17	7.329200418436006
1107	18-19	6.498232887729802
1107	20-21	5.916483798889345
1107	22-23	6.080525458866401
1107	24-25	4.83955962579223
1107	26-27	7.495121576144026
1107	28-29	6.709843762978988
1107	30-31	5.372573633872342
1107	32-33	5.3606817921586085
1107	34-35	6.8521495988499055
1107	36-37	6.79682564319187
1107	38-39	6.725495497639795
1107	40-41	8.096403531643599
1107	42-43	6.451557760855742
1107	44-45	4.471841087845693
1107	46-47	3.8730585421676764
1107	48-49	4.427466198327213
1107	50-51	5.240587534703625
1107	52-53	4.254523127676908
1107	54-55	5.504824471358372
1107	56-57	5.495756474889692
1107	58-59	3.128085168402322
1107	60-61	3.177099788406707
1107	62-63	0.7713734804592534
1107	64-65	2.2727858505271676
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2103	6	9.43143176652194
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2103	68-69	5.658374899580277
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2103	86-87	2.955474489937963
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2104	5	10.498354383447076
2104	6	9.480732739411373
2104	7	8.861902691969142
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2207	16-17	7.537015254581949
2207	18-19	4.1997296176201
2207	20-21	7.660787107025051
2207	22-23	6.450853677800421
2207	24-25	4.357276742368459
2207	26-27	3.9578986051750604
2207	28-29	5.209031612455725
2207	30-31	5.66458736964902
2207	32-33	6.915057947277742
2207	34-35	6.510956750062306
2207	36-37	6.952740230549427
2207	38-39	6.889679642790984
2207	40-41	4.350622412080181
2207	42-43	5.906695486638185
2207	44-45	4.105768813951244
2207	46-47	5.833224016731293
2207	48-49	2.688815038202474
2207	50-51	5.623647404737888
2207	52-53	3.2155318286296293
2207	54-55	3.3051063849650575
2207	56-57	5.327529509025158
2207	58-59	3.9015050913777856
2207	60-61	3.779799951786771
2207	62-63	4.366998013797108
2207	64-65	3.4838202409131434
2207	66-67	3.726023424223012
2207	68-69	3.756280070366458
2207	70-71	3.9139120747915825
2207	72-73	3.9949180203919106
2207	74-75	3.651188609755227
2207	76-77	4.183278256816372
2207	78-79	4.307710928023962
2207	80-81	4.2815961245370815
2207	82-83	3.916719298286935
2207	84-85	3.4018164077888606
2207	86-87	3.5457339983111638
2207	88-89	3.3511426279461105
2207	90-91	1.468258598405047
2207	92-93	-0.5909495901500073
2207	94-95	1.4235783883279858
2207	96-97	-0.8917635633059398
2207	98-99	0.8213983093813546
2207	100	2.195189550526024
2208	1	0.9521202731718574
2208	2	8.417903315515858
2208	3	6.674892106179076
2208	4	11.838108114973299
2208	5	10.055132345405337
2208	6	9.257721026301049
2208	7	7.592451407491225
2208	8	7.896034440500333
2208	9	6.53880154447781
2208	10-11	6.743210078280512
2208	12-13	7.020533181470597
2208	14-15	7.988931811300091
2208	16-17	7.323702718855362
2208	18-19	6.567743597562174
2208	20-21	6.735356306441297
2208	22-23	5.838574593832501
2208	24-25	6.134591745521359
2208	26-27	8.040188846486412
2208	28-29	7.129062847505498
2208	30-31	6.044520902901866
2208	32-33	8.338773218055731
2208	34-35	6.539668800608325
2208	36-37	6.948210540325984
2208	38-39	7.188620019766599
2208	40-41	7.613837747046883
2208	42-43	7.010345574869433
2208	44-45	5.941478874569057
2208	46-47	4.396986108547168
2208	48-49	4.695835928066135
2208	50-51	5.971164175237508
2208	52-53	5.659992490523852
2208	54-55	6.357960373352729
2208	56-57	6.499454602296954
2208	58-59	5.679644404876356
2208	60-61	5.232026396607061
2208	62-63	3.3365277877634867
2208	64-65	3.4639564874740536
2208	66-67	3.0326126139233764
2208	68-69	5.653090575387221
2208	70-71	5.441718643297701
2208	72-73	4.705473808266834
2208	74-75	2.1134649669281274
2208	76-77	3.305541189265
2208	78-79	4.112485552485193
2208	80-81	3.473560355521151
2208	82-83	1.343971497078826
2208	84-85	2.1004425491628176
2208	86-87	1.0251334490910367
2208	88-89	0.7794758706230382
2208	90-91	1.9053925773152116
2208	92-93	0.9014535333720168
2208	94-95	-0.9392697676021271
2208	96-97	1.3880151442449318
2208	98-99	0.2141829384456777
2208	100	1.8182889747567543
2209	1	5.323459618832615
2209	2	9.76668887881991
2209	3	5.826555635892266
2209	4	-3.45235383683449
2209	5	0.3154533867842879
2209	6	4.544674256458414
2209	7	2.6086885310108308
2209	8	5.505228075798957
2209	9	5.2740536734018555
2209	10-11	5.284199830093947
2209	12-13	7.338774998134955
2209	14-15	6.8197361862614585
2209	16-17	6.515382180435427
2209	18-19	5.462535513239747
2209	20-21	6.159056381137219
2209	22-23	5.664470838598856
2209	24-25	5.362364512562902
2209	26-27	7.076451407010804
2209	28-29	6.3890421176742755
2209	30-31	4.185237307481039
2209	32-33	6.350418898904035
2209	34-35	5.718190908087017
2209	36-37	6.372382248490023
2209	38-39	6.862064571070999
2209	40-41	7.361236451169905
2209	42-43	6.862511891319343
2209	44-45	6.609952432637542
2209	46-47	6.862523051279638
2209	48-49	5.293828941907368
2209	50-51	4.86409113352515
2209	52-53	5.108280026770256
2209	54-55	5.506308945885873
2209	56-57	5.133615967559422
2209	58-59	3.3655227119013027
2209	60-61	4.28529311913649
2209	62-63	4.364366324791554
2209	64-65	5.058848128067886
2209	66-67	4.584091147859638
2209	68-69	3.957908538309386
2209	70-71	3.835484582189249
2209	72-73	0.9804526238664337
2209	74-75	2.424391255305636
2209	76-77	3.4998516622421434
2209	78-79	3.5963516966363613
2209	80-81	3.7245533283561265
2209	82-83	3.0985993757018946
2209	84-85	3.0374373673750057
2209	86-87	1.5554907122977184
2209	88-89	2.032333527381759
2209	90-91	2.3301897573537147
2209	92-93	2.419839697798512
2209	94-95	0.8243436358127525
2209	96-97	0.20295740671250329
2209	98-99	0.40730298574220214
2209	100	0.22771377923150382
2210	1	1.6490098857717967
2210	2	-17.16649701339287
2210	3	-7.697989789606103
2210	4	-8.856803676873604
2210	5	-2.278986111783656
2210	6	2.3247230243908277
2210	7	-9.602098044247725
2210	8	-9.60627074109905
2210	9	-4.353594650407693
2210	10-11	-1.6142299570412106
2210	12-13	3.131736338305167
2210	14-15	-6.195703057400593
2210	16-17	-2.1118174987288754
2210	18-19	-0.6945071157681078
2210	20-21	2.062166725421001
2210	22-23	2.119851272892472
2210	24-25	1.4824023315149653
2210	26-27	3.8157343871256586
2210	28-29	3.151744762723201
2210	30-31	2.235881531117247
2210	32-33	4.201547197546052
2210	34-35	1.9381239237349632
2210	36-37	3.636844332230229
2210	38-39	2.5306350676054272
2210	40-41	3.7771718987674348
2210	42-43	1.8496044731681849
2210	44-45	2.8341701857490733
2210	46-47	2.526759651699347
2210	48-49	2.568939102981961
2210	50-51	1.969308004384569
2210	52-53	2.0445134719679317
2210	54-55	2.3296099499302905
2210	56-57	2.3489006966631045
2210	58-59	1.3407901363804733
2210	60-61	2.3888044212514785
2210	62-63	1.2431972015325137
2210	64-65	2.1747282197121507
2210	66-67	2.3191364503329694
2210	68-69	3.0345360182132346
2210	70-71	2.125929408559582
2210	72-73	0.8066494010142833
2210	74-75	0.17519813554017816
2210	76-77	-0.3740017660189565
2210	78-79	0.005531535861447168
2210	80-81	0.46254294267670915
2210	82-83	0.5024512708436149
2210	84-85	-1.5980156339789424
2210	86-87	-1.4179357113973907
2210	88-89	-1.5961806652725485
2210	90-91	-2.0282558565468882
2210	92-93	-1.4817030602563328
2210	94-95	-0.696872993601616
2210	96-97	-0.2767504582395155
2210	98-99	-0.6298934996595484
2210	100	-0.4492763163497031
2211	1	0.8790745869708765
2211	2	-17.16649701339287
2211	3	-8.072137714783151
2211	4	-19.153669166830998
2211	5	-21.542071930408124
2211	6	-14.16773775958855
2211	7	-12.015548720803162
2211	8	-18.717827737840995
2211	9	-16.448337822394976
2211	10-11	-10.59030280787642
2211	12-13	-22.677986729254176
2211	14-15	-18.11875939423341
2211	16-17	-15.7583452497379
2211	18-19	-19.45076855975659
2211	20-21	-9.201660262079663
2211	22-23	-4.888495791653316
2211	24-25	-2.5175990987232346
2211	26-27	-17.670660617782694
2211	28-29	-4.093306279839911
2211	30-31	-5.3875655079119795
2211	32-33	-1.5033928285392726
2211	34-35	-6.971201404300579
2211	36-37	-7.975281593828182
2211	38-39	-3.442539356913933
2211	40-41	-6.549975316456031
2211	42-43	-2.5772456052088657
2211	44-45	-0.4681694506671583
2211	46-47	0.5047585057314024
2211	48-49	0.28216995609155404
2211	50-51	-5.768499395462378
2211	52-53	-1.5785120355944429
2211	54-55	-0.8445786634407249
2211	56-57	-8.060119240801878
2211	58-59	-3.063284412300227
2211	60-61	-1.6402437860518546
2211	62-63	-0.8949974401675469
2211	64-65	-4.522330388505559
2211	66-67	-3.315642088251302
2211	68-69	-0.16162588619531348
2211	70-71	-0.40476094825791264
2211	72-73	-1.380387069893228
2211	74-75	-2.066183512887388
2211	76-77	-2.091594595698858
2211	78-79	-1.4290308451302387
2211	80-81	-1.2905820583647234
2211	82-83	-1.7341954698740238
2211	84-85	-1.8199551797264029
2211	86-87	-2.2716662641847307
2211	88-89	-2.8779096527405486
2211	90-91	-2.567135229645988
2211	92-93	-2.42543351806475
2211	94-95	-2.535949256346427
2211	96-97	-3.252941879987379
2211	98-99	-3.2224215937035687
2211	100	-2.62141323219182
2212	1	-21.340491027192293
2212	2	-17.16649701339287
2212	3	-20.10791066605163
2212	4	-19.153669166830998
2212	5	-21.542071930408124
2212	6	-22.329263585469185
2212	7	-22.657636693060873
2212	8	-23.058035805006536
2212	9	-24.466954358259184
2212	10-11	-11.692449981115447
2212	12-13	-22.677986729254176
2212	14-15	-24.056704274686048
2212	16-17	-24.42990940378061
2212	18-19	-19.06325909412844
2212	20-21	-24.15557521006287
2212	22-23	-19.225214947544785
2212	24-25	-25.842384643530682
2212	26-27	-17.697236666238897
2212	28-29	-18.601583769155614
2212	30-31	-6.707945048055443
2212	32-33	-17.85155755904301
2212	34-35	-14.396959212192167
2212	36-37	-18.24314446135953
2212	38-39	-24.3362243359247
2212	40-41	-10.778212146105986
2212	42-43	-14.583783014527295
2212	44-45	-17.74094805436673
2212	46-47	-17.773407222825234
2212	48-49	-18.386939321583533
2212	50-51	-9.606663611472285
2212	52-53	-2.584500190188116
2212	54-55	-24.225852618542017
2212	56-57	-24.159772462192645
2212	58-59	-13.161144720387597
2212	60-61	-6.808525111277241
2212	62-63	-3.6855297035708006
2212	64-65	-17.979620806758106
2212	66-67	-18.28743602240163
2212	68-69	-15.134493256361736
2212	70-71	-10.213211639641557
2212	72-73	-18.144065197669722
2212	74-75	-6.8857568770208815
2212	76-77	-11.788455157614038
2212	78-79	-5.476306233976043
2212	80-81	-2.998733317596514
2212	82-83	-3.5633046759107323
2212	84-85	-5.797011604232004
2212	86-87	-5.353249436589497
2212	88-89	-3.8189945329880395
2212	90-91	-4.539157673950815
2212	92-93	-5.7686709118669555
2212	94-95	-4.045032921672103
2212	96-97	-5.939177574494618
2212	98-99	-4.228979761871088
2212	100	-4.21334391749923
2213	1	-21.340491027192293
2213	2	-17.16649701339287
2213	3	-20.10791066605163
2213	4	-19.153669166830998
2213	5	-21.542071930408124
2213	6	-22.329263585469185
2213	7	-10.820162270377446
2213	8	-10.686507012255879
2213	9	-4.2395354748917455
2213	10-11	-18.034816069668047
2213	12-13	-16.667901958719245
2213	14-15	-24.056704274686048
2213	16-17	-18.380947112053043
2213	18-19	-19.062605955005832
2213	20-21	-18.105151357388216
2213	22-23	-20.304214142056512
2213	24-25	-7.917655032305895
2213	26-27	-23.773507852679575
2213	28-29	-24.69168189582823
2213	30-31	-20.00447685910547
2213	32-33	-23.955482625947557
2213	34-35	-18.491589872742093
2213	36-37	-24.36513375663161
2213	38-39	-12.04084286851751
2213	40-41	-17.696730253685736
2213	42-43	-23.919643853064315
2213	44-45	-17.75246452880532
2213	46-47	-17.648624859811044
2213	48-49	-6.578565933309765
2213	50-51	-24.007867893363454
2213	52-53	-18.43970109158399
2213	54-55	-18.36075469395856
2213	56-57	-18.250681553101735
2213	58-59	-18.690504102970014
2213	60-61	-20.00854500728086
2213	62-63	-18.225847729324002
2213	64-65	-18.257833952670914
2213	66-67	-9.181047423463603
2213	68-69	-23.331638662249336
2213	70-71	-17.99967080550617
2213	72-73	-10.006166140031027
2213	74-75	-4.678799064632113
2213	76-77	-3.469917792893174
2213	78-79	-5.137356951575089
2213	80-81	-3.745546241185167
2213	82-83	-3.5647569146805083
2213	84-85	-3.3627144403382196
2213	86-87	-3.4674754442386373
2213	88-89	-3.1256922761866974
2213	90-91	-3.210163173122769
2213	92-93	-4.135320098907904
2213	94-95	-4.1697519199565605
2213	96-97	-3.806853765601602
2213	98-99	-4.164381245231585
2213	100	-3.530913079580209
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1025.0
3	1081.0
4	8914.0
5	29832.0
6	32205.0
7	37720.0
8	34474.0
9	33308.0
10	37768.0
11	45511.0
12	54785.0
13	71511.0
14	53020.0
15	58471.0
16	67720.0
17	71522.0
18	71851.0
19	71533.0
20	68756.0
21	65815.0
22	68776.0
23	75832.0
24	84188.0
25	93162.0
26	103130.0
27	112183.0
28	122703.0
29	130310.0
30	138784.0
31	152946.0
32	173995.0
33	213187.0
34	260446.0
35	311249.0
36	347945.0
37	308606.0
38	98126.0
39	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6435845189648	21.727319961416537	13.932349417401	23.69674610221766
2	14.799684888452136	21.694669609085963	40.65913539238697	22.84651011007493
3	17.29708882553183	27.508015444375072	30.113620652267247	25.081275077825854
4	12.033609185151036	18.208553740120518	40.08945885578404	29.668378218944408
5	12.68226524886391	38.024405437217695	36.64546258504252	12.64786672887587
6	29.28180200022269	39.58302510421322	18.795595666438388	12.339577229125707
7	25.818490139878637	32.2688464479976	24.84721993895945	17.065443473164315
8	23.39902065659933	36.6170449495601	22.45843651260945	17.525497881231125
9	24.27493900305662	17.139665564408336	22.690978216324297	35.89441721621075
10-11	22.686988299702822	27.855116435280646	29.75063343341789	19.70726183159864
12-13	23.560189640471748	25.489246332099984	29.780352623036844	21.170211404391424
14-15	21.183714199353236	26.87245242304846	27.005262108735657	24.938571268862642
16-17	20.626039873364384	30.575694144785214	27.91945517261712	20.878810809233286
18-19	20.596820689331174	29.59842312138661	29.487267112061506	20.317489077220717
20-21	22.05591089266603	29.030497797769932	28.288333613666776	20.625257695897258
22-23	20.89498405287879	29.176805492594966	28.744389270705064	21.183821183821184
24-25	21.291748685249008	28.881648352674276	28.223077138521575	21.603525823555138
26-27	21.353624321194225	28.77254147832819	28.391191714967977	21.482642485509608
28-29	21.01830898241348	28.700681816025703	28.915041913842916	21.365967287717897
30-31	21.801336936416323	29.12022356047502	28.093293768175048	20.98514573493361
32-33	20.65894967102976	28.994314148370975	28.921328123392637	21.425408057206628
34-35	20.806661440171034	28.36789737727223	28.80708796463146	22.01835321792528
36-37	21.543454090559667	29.04798160226753	28.233707442568985	21.174856864603818
38-39	21.297349195026623	28.676199146686944	28.47160574355612	21.55484591473032
40-41	21.430267025100843	28.817014933662506	28.096070750439456	21.656647290797192
42-43	21.116451625093877	28.870409066575075	28.718705136849437	21.294434171481615
44-45	21.643683114796012	28.25743818254867	28.526228131049347	21.572650571605976
46-47	21.415803476680615	28.85891864526687	28.688723558640383	21.036554319412133
48-49	21.22611860189939	28.50154141093226	28.859987021186935	21.412352965981416
50-51	21.538173172033755	28.65731589409699	28.407701668874974	21.396809264994285
52-53	20.867476198499997	28.731526898559217	28.59027805619336	21.81071884674743
54-55	21.36604803393433	28.68850605119466	28.20749677055849	21.73794914431252
56-57	21.558586432103482	28.592991586600565	28.175906931196703	21.67251505009925
58-59	21.158158544968664	28.095529810604386	28.767020396591846	21.979291247835103
60-61	21.714247393447213	28.666092002934224	28.125820952417758	21.493839651200805
62-63	20.998852237689363	28.77799504620533	28.679916842426795	21.543235873678515
64-65	21.010754138870052	28.18704887825349	28.703148133026108	22.09904884985035
66-67	21.334000319142156	28.48824250026352	28.32539389682987	21.852363283764458
68-69	21.80571531937698	28.301184619831552	28.28689768959311	21.606202371198354
70-71	21.776245685246963	28.50042475797427	27.98450600967797	21.7388235471008
72-73	21.39268615627468	28.562857135783055	28.576020833029574	21.46843587491269
74-75	21.769897045338038	27.978383809914774	28.158396186531544	22.093322958215644
76-77	21.617689611165503	28.566291278495303	28.302385553034075	21.513633557305116
78-79	21.640322864022984	28.243339982154975	28.505131305614984	21.611205848207057
80-81	21.706773486195765	28.23415577464302	28.21382322979436	21.845247509366853
82-83	21.03843886879398	28.534616424975273	28.606939012634353	21.820005693596396
84-85	21.32844405710869	28.082707210083257	28.148707504514476	22.440141228293577
86-87	21.629640118083547	28.388472017968102	28.146530158737033	21.835357705211315
88-89	21.37171229743516	27.92352502662552	28.571694472367813	22.133068203571508
90-91	21.762096370750754	27.998896703902908	28.148436591572224	22.090570333774114
92-93	21.16831836261741	28.331515165711412	28.530780199037565	21.969386272633614
94-95	21.122841421943754	27.86290131518298	28.46935532288028	22.544901939992986
96-97	21.427378792708097	28.10451066131135	28.38022674121539	22.08788380476516
98-99	21.409957275663377	27.81044207162251	28.409408833307374	22.370191819406735
100	21.51463401711004	27.66840701628538	28.140923485753177	22.6760354808514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	412.0
1	479.0
2	450.0
3	376.5
4	472.0
5	733.0
6	1271.5
7	2070.0
8	3130.5
9	4440.0
10	6076.0
11	7800.0
12	9111.0
13	10288.5
14	11527.5
15	12729.0
16	13708.5
17	14736.5
18	15861.5
19	16878.5
20	18182.5
21	19743.0
22	21499.5
23	23495.5
24	26287.0
25	30201.0
26	35336.0
27	42129.0
28	50823.5
29	61351.5
30	72972.0
31	85176.5
32	97221.5
33	108587.5
34	118678.5
35	127170.5
36	134174.0
37	139866.5
38	144565.5
39	147041.0
40	146967.5
41	144629.0
42	140316.5
43	134918.0
44	128409.0
45	121568.5
46	115083.5
47	109333.0
48	105051.0
49	102834.0
50	102968.5
51	97024.5
52	84680.0
53	74433.0
54	66346.5
55	59907.5
56	54164.5
57	48492.0
58	43021.5
59	37432.0
60	31982.0
61	26607.0
62	21550.5
63	17400.0
64	13943.0
65	11038.5
66	8652.0
67	6638.0
68	5070.5
69	3901.0
70	2937.5
71	2203.0
72	1671.0
73	1262.5
74	943.0
75	695.0
76	504.5
77	371.0
78	269.0
79	166.0
80	113.5
81	95.5
82	67.5
83	41.5
84	28.5
85	21.5
86	13.5
87	7.5
88	9.5
89	11.0
90	8.5
91	5.0
92	3.5
93	2.0
94	1.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	9.331855510993437
2	27.036822556273517
3	7.464035316901313
4	24.981455394635127
5	13.867593941826234
6	11.704110518844928
7	9.559212103418833
8	10.841065863650687
9	6.540414310994514
10-11	8.072963662270011
12-13	11.611374024623789
14-15	8.929315021265019
16-17	9.553474992390921
18-19	7.980792798713594
20-21	9.523469632367004
22-23	6.95262439161035
24-25	6.235337371756714
26-27	11.472161544117846
28-29	7.727390260917345
30-31	5.195896753549333
32-33	10.32317712050896
34-35	8.559567642693802
36-37	8.621234852545474
38-39	7.337899678667912
40-41	9.795080090608646
42-43	7.701452054720727
44-45	7.8190358959993755
46-47	7.292258670983416
48-49	4.827226766720447
50-51	8.247797407256773
52-53	5.608268442926519
54-55	5.920429232870145
56-57	5.905574694222756
58-59	4.611788820444586
60-61	5.21216536885853
62-63	3.620117058612804
64-65	5.169662264623573
66-67	3.0272957197919554
68-69	6.49035599716646
70-71	3.26484713157916
72-73	2.0921062200518765
74-75	1.088771692609362
76-77	1.4691717820328767
78-79	2.268111628558423
80-81	2.5010975928374997
82-83	1.269935114082318
84-85	1.7496572547519709
86-87	0.5121381647170365
88-89	0.8742899315048199
90-91	0.3948640735643082
92-93	0.534642184734975
94-95	0.8067105344671086
96-97	0.7905900605224812
98-99	0.549335114621014
100	0.8720139414491457
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3712670.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.18288711240315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.93900028845808	92.26933921245521
2	2.5513036119886436	4.856808873787629
3	0.3099191423290657	0.8849699621484078
4	0.08704048440540016	0.3313905840547236
5	0.03584201391595353	0.17057731822216934
6	0.020245139531524115	0.11561944983623461
7	0.01549036428610989	0.10320923166223593
8	0.009157850279831058	0.06973365035019594
9	0.00657383124381497	0.05631446134584262
>10	0.023082655612845213	0.3633524373742478
>50	0.0010542692783238777	0.06838600854360423
>100	9.770781029497624E-4	0.2509390136365692
>500	1.4239571363260396E-4	0.10897122608384407
>1k	1.7087485390304715E-4	0.35038857049908184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4127	0.11115989301499998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6934793558274773E-5	0.0	0.0	0.0
3	0.0	2.6934793558274773E-5	0.0	0.0	0.0
4	0.0	2.6934793558274773E-5	0.0	2.6934793558274773E-5	0.0
5	0.0	5.3869587116549546E-5	0.0	2.6934793558274773E-5	0.0
6	2.6934793558274773E-5	5.3869587116549546E-5	0.0	2.6934793558274773E-5	0.0
7	2.6934793558274773E-5	5.3869587116549546E-5	0.0	2.6934793558274773E-5	0.0
8	2.6934793558274773E-5	5.3869587116549546E-5	0.0	2.6934793558274773E-5	0.0
9	2.6934793558274773E-5	5.3869587116549546E-5	0.0	2.6934793558274773E-5	0.0
10-11	2.6934793558274773E-5	5.3869587116549546E-5	0.0	2.6934793558274773E-5	0.0
12-13	2.6934793558274773E-5	8.080438067482431E-5	0.0	4.0402190337412156E-5	0.0
14-15	2.6934793558274773E-5	1.0773917423309909E-4	0.0	8.080438067482431E-5	0.0
16-17	4.0402190337412156E-5	1.0773917423309909E-4	0.0	1.0773917423309909E-4	0.0
18-19	5.3869587116549546E-5	1.0773917423309909E-4	0.0	1.0773917423309909E-4	0.0
20-21	5.3869587116549546E-5	1.0773917423309909E-4	0.0	1.0773917423309909E-4	0.0
22-23	5.3869587116549546E-5	1.0773917423309909E-4	0.0	1.885435549079234E-4	0.0
24-25	5.3869587116549546E-5	1.0773917423309909E-4	0.0	2.6934793558274774E-4	0.0
26-27	6.733698389568694E-5	1.0773917423309909E-4	0.0	2.6934793558274774E-4	0.0
28-29	8.080438067482431E-5	1.0773917423309909E-4	0.0	5.386958711654955E-4	0.0
30-31	8.080438067482431E-5	1.0773917423309909E-4	0.0	0.0017507615812878603	0.0
32-33	8.080438067482431E-5	1.0773917423309909E-4	0.0	0.0058044480118082135	0.0
34-35	8.080438067482431E-5	1.2120657101223648E-4	0.0	0.012605483385272592	0.0
36-37	8.080438067482431E-5	1.3467396779137387E-4	0.0	0.02284070493741701	0.0
38-39	8.080438067482431E-5	1.3467396779137387E-4	0.0	0.0387591679303574	0.0
40-41	8.080438067482431E-5	1.3467396779137387E-4	0.0	0.06038780715765204	0.0
42-43	8.080438067482431E-5	1.885435549079234E-4	0.0	0.0813026743556524	0.0
44-45	8.080438067482431E-5	2.0201095168706078E-4	0.0	0.10695806521990912	0.0
46-47	8.080438067482431E-5	2.289457452453356E-4	0.0	0.1377580016537963	0.0
48-49	8.080438067482431E-5	2.4241314202447296E-4	0.0	0.1701336235108426	0.0
50-51	8.080438067482431E-5	2.4241314202447296E-4	0.0	0.20628011646604735	0.0
52-53	8.080438067482431E-5	2.4241314202447296E-4	0.0	0.24374641430560756	0.0
54-55	8.080438067482431E-5	2.5588053880361034E-4	0.0	0.2866670078407184	0.0
56-57	8.080438067482431E-5	2.8281533236188515E-4	0.0	0.33638863674929365	0.0
58-59	8.080438067482431E-5	2.962827291410225E-4	0.0	0.3926419530957505	0.0
60-61	8.080438067482431E-5	3.2321752269929725E-4	0.0	0.45293548847594856	0.0
62-63	8.080438067482431E-5	3.2321752269929725E-4	0.0	0.5134849043949503	0.0
64-65	8.080438067482431E-5	3.2321752269929725E-4	0.0	0.5862088470022921	0.0
66-67	8.080438067482431E-5	3.2321752269929725E-4	0.0	0.6717133491530354	0.0
68-69	8.080438067482431E-5	3.2321752269929725E-4	0.0	0.7677493555850641	0.0
70-71	8.080438067482431E-5	3.3668491947843465E-4	0.0	0.8844308812795105	0.0
72-73	8.080438067482431E-5	3.770871098158468E-4	0.0	1.003011309919815	0.0
74-75	8.080438067482431E-5	3.770871098158468E-4	0.0	1.1284062413303633	0.0
76-77	8.080438067482431E-5	3.770871098158468E-4	0.0	1.26912707027557	0.0
78-79	8.080438067482431E-5	3.770871098158468E-4	0.0	1.4288099938858019	0.0
80-81	8.080438067482431E-5	3.905545065949842E-4	0.0	1.6098926109780831	0.0
82-83	8.080438067482431E-5	4.040219033741216E-4	0.0	1.8064896691599306	0.0
84-85	8.080438067482431E-5	4.040219033741216E-4	0.0	2.0110324914414694	0.0
86-87	8.080438067482431E-5	4.3095669693239637E-4	0.0	2.234685549752604	0.0
88	8.080438067482431E-5	4.3095669693239637E-4	0.0	2.4215726148566934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	1580	0.0	17.164038	1
ATACCGA	245	4.428083E-5	16.453856	6
GTATATA	2740	0.0	15.746039	1
GTACTAA	940	0.0	15.736467	1
GTACATG	15795	0.0	15.062308	1
TACATGG	15785	0.0	14.678143	2
GTATAGC	840	0.0	14.674879	1
TATACTG	1710	0.0	14.39169	5
TACCGAT	285	1.6614574E-4	14.090829	7
CATGGGG	4135	0.0	14.010621	4
ACATGGG	15985	0.0	13.905147	3
GTATAAT	1795	0.0	13.734707	1
CTACACT	1540	0.0	13.383835	4
GTATAGA	1475	0.0	13.3715515	1
ATAGGAC	965	0.0	13.277487	3
TACTGCG	380	9.601328E-6	13.210154	7
GTATTAT	2100	0.0	13.207392	1
GTATAGG	995	0.0	13.0082855	1
TCTATAC	1335	0.0	12.935874	3
GTAGGAC	735	1.07320375E-10	12.884776	3
>>END_MODULE