FastQCFastQC Report
Wed 25 May 2016
SRR1295124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712670
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT112950.30422849324071355No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA84330.22714111407693116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG3106.348273E-918.1999265
GTCGTAA1750.001477738417.2497431
GTCCTAT11700.016.3405671
GAGTACT41400.016.09991612-13
TATACTG21600.015.236825
GTATATA29550.014.8125941
GTACTAG8500.014.7975731
TATACAG23950.013.7417695
TAGCCCG2400.001191132213.71314055
CTATACA20600.013.4658844
TACCGTA2802.526491E-413.4113787
GTACATG212350.013.4101481
CCTATAC11600.013.3748363
AGTACTT51550.013.20307212-13
ACTGTCG3256.0985607E-512.998728
GTATAGG10850.012.9836771
ACATGGG203850.012.9385213
TACATGG213550.012.8046442
CATGGGT30550.012.7737084
CGCACTG4455.3502845E-712.67865
Produced by FastQC (version 0.11.5)