##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295124_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3712670 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.318584199511402 33.0 33.0 33.0 27.0 33.0 2 29.759185437973212 33.0 33.0 33.0 22.0 33.0 3 30.718667697371433 33.0 33.0 33.0 27.0 33.0 4 35.092040499155594 37.0 37.0 37.0 33.0 37.0 5 35.43357368147452 37.0 37.0 37.0 33.0 37.0 6 35.20975739831442 37.0 37.0 37.0 33.0 37.0 7 35.11581718816916 37.0 37.0 37.0 33.0 37.0 8 35.10395914530513 37.0 37.0 37.0 33.0 37.0 9 35.074735971686145 37.0 37.0 37.0 33.0 37.0 10-11 35.04969725292041 37.0 37.0 37.0 33.0 37.0 12-13 34.96823525926085 37.0 37.0 37.0 33.0 37.0 14-15 36.42493865600767 40.0 37.0 40.0 33.0 40.0 16-17 36.41903683871715 38.5 37.0 40.0 33.0 40.0 18-19 36.44537758540349 40.0 37.0 40.0 33.0 40.0 20-21 36.441891953769115 40.0 37.0 40.0 33.0 40.0 22-23 36.40337519898078 40.0 37.0 40.0 33.0 40.0 24-25 36.374983367764976 40.0 37.0 40.0 33.0 40.0 26-27 36.34877769906832 38.5 37.0 40.0 33.0 40.0 28-29 36.30097988778965 37.0 37.0 40.0 30.0 40.0 30-31 36.217004743217146 37.0 37.0 40.0 27.0 40.0 32-33 36.162839008045424 37.0 37.0 40.0 27.0 40.0 34-35 36.06474518338554 37.0 37.0 40.0 27.0 40.0 36-37 36.071452485677426 37.0 37.0 40.0 27.0 40.0 38-39 36.02254832775334 37.0 37.0 40.0 27.0 40.0 40-41 35.960026476902065 37.0 37.0 40.0 27.0 40.0 42-43 35.87683338406053 37.0 37.0 40.0 27.0 40.0 44-45 35.778218909841165 37.0 37.0 40.0 27.0 40.0 46-47 35.68117419539038 37.0 37.0 40.0 27.0 40.0 48-49 35.583045490172836 37.0 37.0 40.0 27.0 40.0 50-51 35.49228978066998 37.0 37.0 40.0 27.0 40.0 52-53 35.39998585923338 37.0 35.0 40.0 27.0 40.0 54-55 35.29108687278966 37.0 33.0 40.0 27.0 40.0 56-57 35.16372529742746 37.0 33.0 40.0 27.0 40.0 58-59 35.04059302334977 37.0 33.0 40.0 27.0 40.0 60-61 34.88087319907237 37.0 33.0 40.0 27.0 40.0 62-63 34.70594881850528 37.0 33.0 40.0 27.0 40.0 64-65 34.529892233890976 37.0 33.0 40.0 27.0 40.0 66-67 34.3060185796206 37.0 33.0 40.0 27.0 40.0 68-69 34.090705341438905 37.0 33.0 38.5 27.0 40.0 70-71 33.85277334101873 37.0 33.0 37.0 27.0 40.0 72-73 33.63626096582783 37.0 33.0 37.0 24.5 40.0 74-75 33.39660796677324 37.0 33.0 37.0 22.0 40.0 76-77 33.15241039467553 37.0 33.0 37.0 22.0 38.5 78-79 32.91048733121985 37.0 33.0 37.0 22.0 37.0 80-81 32.69960271179501 37.0 33.0 37.0 22.0 37.0 82-83 32.48730630516582 37.0 33.0 37.0 22.0 37.0 84-85 32.307844085254004 35.0 33.0 37.0 22.0 37.0 86-87 32.32587113856066 33.0 33.0 37.0 22.0 37.0 88-89 32.337332835937474 33.0 33.0 37.0 22.0 37.0 90-91 32.34418316198315 33.0 33.0 37.0 22.0 37.0 92-93 32.30115590666556 33.0 33.0 37.0 22.0 37.0 94-95 32.328901572183895 33.0 33.0 37.0 22.0 37.0 96-97 32.28893195463104 33.0 33.0 37.0 22.0 37.0 98-99 32.21275402877174 33.0 33.0 37.0 22.0 37.0 100 31.67815157285727 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5977934529135425 1101 2 4.184837359688942 1101 3 2.3902894041361584 1101 4 1.1165347969727435 1101 5 0.5174330925196742 1101 6 0.5631089745992028 1101 7 0.6396656207561335 1101 8 0.5082951592498262 1101 9 0.5743407046536078 1101 10-11 0.6357820378623344 1101 12-13 0.683474567912107 1101 14-15 0.9240046406738287 1101 16-17 0.9400137739117085 1101 18-19 0.938581476932292 1101 20-21 0.8712636057978571 1101 22-23 0.887268906592098 1101 24-25 0.8776064841800277 1101 26-27 0.9078717441236606 1101 28-29 0.8236342854102574 1101 30-31 0.7544088493650918 1101 32-33 0.9267569472850639 1101 34-35 1.0571680357375541 1101 36-37 0.9617027412283576 1101 38-39 0.9181417061594885 1101 40-41 0.9304736199944372 1101 42-43 1.0759627142992727 1101 44-45 1.0316766169217502 1101 46-47 1.018875707837509 1101 48-49 0.9013089307514761 1101 50-51 0.9632275889696373 1101 52-53 0.9497440983109158 1101 54-55 0.9337443326729797 1101 56-57 0.8956490036918581 1101 58-59 0.9038388561873134 1101 60-61 0.8489020255023121 1101 62-63 0.9210899378803106 1101 64-65 0.8845611767213555 1101 66-67 0.8732597374979605 1101 68-69 0.9301720424350535 1101 70-71 0.9732940331952946 1101 72-73 0.8369255490969394 1101 74-75 0.7931447393681594 1101 76-77 0.7973488552199939 1101 78-79 0.919266936247368 1101 80-81 0.8087070325465291 1101 82-83 0.7811125073263838 1101 84-85 0.8693768167990399 1101 86-87 0.97858623132921 1101 88-89 0.7929042984305141 1101 90-91 0.800836108137176 1101 92-93 0.8954910108864595 1101 94-95 0.8308247305089509 1101 96-97 0.773401684617582 1101 98-99 0.7627917420982655 1101 100 0.9160213432299926 1102 1 0.4663800725728038 1102 2 4.037105985737007 1102 3 2.278881054710098 1102 4 1.0272807915718545 1102 5 0.3995826274863248 1102 6 0.4301670542579572 1102 7 0.43847041963537237 1102 8 0.4360351562286695 1102 9 0.48258150276156897 1102 10-11 0.5017293196186827 1102 12-13 0.6071331864203273 1102 14-15 0.7896497820728712 1102 16-17 0.7993144898219597 1102 18-19 0.7810975753698628 1102 20-21 0.7772830201693779 1102 22-23 0.7573262123643758 1102 24-25 0.7917768122889726 1102 26-27 0.8161961379587979 1102 28-29 0.7472503760502391 1102 30-31 0.7576275744164676 1102 32-33 0.8894165572945596 1102 34-35 0.9453400192849699 1102 36-37 0.780280747648483 1102 38-39 0.7535079433713534 1102 40-41 0.7276093917227229 1102 42-43 0.8337513499785061 1102 44-45 0.9523185381438921 1102 46-47 0.8423178697735878 1102 48-49 0.8120700882403113 1102 50-51 0.8031533699277915 1102 52-53 0.8294656296795822 1102 54-55 0.797798561496073 1102 56-57 0.7283800464908481 1102 58-59 0.6947554206533297 1102 60-61 0.7784512513058814 1102 62-63 0.8840906027962703 1102 64-65 0.8625420833887532 1102 66-67 0.8127584616619146 1102 68-69 0.7741438190689678 1102 70-71 0.8375793920916763 1102 72-73 0.7069471131893934 1102 74-75 0.6991885089783665 1102 76-77 0.6691726335674275 1102 78-79 0.7048989604009819 1102 80-81 0.6921600213894479 1102 82-83 0.7896342063269515 1102 84-85 0.7521075007130591 1102 86-87 0.7370110477024454 1102 88-89 0.6717311866792741 1102 90-91 0.6947986924221254 1102 92-93 0.7876756219644605 1102 94-95 0.6823140052318024 1102 96-97 0.8089590443857304 1102 98-99 0.7708641579063027 1102 100 0.6869610900333925 1103 1 0.5088672031696397 1103 2 4.024368511106623 1103 3 2.2422153769084616 1103 4 0.9948082717825741 1103 5 0.3333940356780474 1103 6 0.2631085443773671 1103 7 0.24489643053205157 1103 8 0.26585368424861855 1103 9 0.37639321153341143 1103 10-11 0.3862814944465285 1103 12-13 0.4748921133231576 1103 14-15 0.6130513492714726 1103 16-17 0.5551876946725329 1103 18-19 0.6796307117143172 1103 20-21 0.66756023345188 1103 22-23 0.6050857142900412 1103 24-25 0.5994345482410992 1103 26-27 0.6141996652011557 1103 28-29 0.5699595961493813 1103 30-31 0.4969718131450449 1103 32-33 0.6942278321855326 1103 34-35 0.7247046386525184 1103 36-37 0.5848677636328077 1103 38-39 0.562257994012576 1103 40-41 0.5599556351900077 1103 42-43 0.540802012104237 1103 44-45 0.584951084175124 1103 46-47 0.5910659209035742 1103 48-49 0.49613166403812414 1103 50-51 0.501554349034862 1103 52-53 0.6303114686705769 1103 54-55 0.5506992903375121 1103 56-57 0.4930962531174643 1103 58-59 0.5391704786718279 1103 60-61 0.4440249607390001 1103 62-63 0.6038234262760227 1103 64-65 0.47666588580568003 1103 66-67 0.5100674201256652 1103 68-69 0.5249563002010333 1103 70-71 0.5243108054586045 1103 72-73 0.4887170596207895 1103 74-75 0.48156340557227395 1103 76-77 0.4529157904689356 1103 78-79 0.4529689419871801 1103 80-81 0.4518882316177937 1103 82-83 0.48575600400516805 1103 84-85 0.49430953760077756 1103 86-87 0.441231813345361 1103 88-89 0.43820046151013514 1103 90-91 0.412114525022524 1103 92-93 0.4397465863169643 1103 94-95 0.39729063839467926 1103 96-97 0.41167148591962643 1103 98-99 0.417424835230932 1103 100 0.39462202781722766 1104 1 0.5235744906035116 1104 2 3.761472666834859 1104 3 2.2479043208423235 1104 4 1.0266273488221245 1104 5 0.44316221612263007 1104 6 0.4413160404427856 1104 7 0.4425747887010374 1104 8 0.394343055820201 1104 9 0.46276714383030537 1104 10-11 0.5496214219165623 1104 12-13 0.525954407819782 1104 14-15 0.7121869032827703 1104 16-17 0.7284833675872093 1104 18-19 0.6820455299367225 1104 20-21 0.7452933181065617 1104 22-23 0.653901534191192 1104 24-25 0.6817062395598725 1104 26-27 0.6795960225093154 1104 28-29 0.6457378461657939 1104 30-31 0.646068341894896 1104 32-33 0.7749158958381059 1104 34-35 0.8491954748429436 1104 36-37 0.7367675326899601 1104 38-39 0.6744297572963731 1104 40-41 0.6846100637663852 1104 42-43 0.66480901901312 1104 44-45 0.6949576076940573 1104 46-47 0.7139376008844565 1104 48-49 0.7064204015077777 1104 50-51 0.6204946130489546 1104 52-53 0.6140974927585816 1104 54-55 0.5861362583897218 1104 56-57 0.5843880777873025 1104 58-59 0.665762510502276 1104 60-61 0.658648693044448 1104 62-63 0.6662381764840859 1104 64-65 0.6231648525409383 1104 66-67 0.5983362595934025 1104 68-69 0.6279389275162259 1104 70-71 0.6290667602442355 1104 72-73 0.5879742014681781 1104 74-75 0.5322249280117575 1104 76-77 0.5120888926264087 1104 78-79 0.6568182428420783 1104 80-81 0.5665506295873328 1104 82-83 0.5531115404082314 1104 84-85 0.6425304386742781 1104 86-87 0.6881533346250279 1104 88-89 0.6253687166858839 1104 90-91 0.5889459103796071 1104 92-93 0.6047093880053112 1104 94-95 0.6178866968693058 1104 96-97 0.6300092730532327 1104 98-99 0.6709260360908118 1104 100 0.5024569576000673 1105 1 0.37817657504438884 1105 2 3.7861236976743697 1105 3 2.1289936522327615 1105 4 0.9200737146376028 1105 5 0.27079325008570976 1105 6 0.24347544518344932 1105 7 0.20234922112728526 1105 8 0.21298423573428238 1105 9 0.1934335228170383 1105 10-11 0.23297138854675126 1105 12-13 0.243292664551376 1105 14-15 0.3173567499122001 1105 16-17 0.31502920291838876 1105 18-19 0.31405110896869104 1105 20-21 0.31134058950891585 1105 22-23 0.3221008695026626 1105 24-25 0.37187357739170324 1105 26-27 0.3405986062995794 1105 28-29 0.32694507255910565 1105 30-31 0.28276501955168243 1105 32-33 0.3372933275965764 1105 34-35 0.4415221309962405 1105 36-37 0.3364780619533505 1105 38-39 0.40358898530976006 1105 40-41 0.3160436916788001 1105 42-43 0.3865951283066309 1105 44-45 0.46297333579772015 1105 46-47 0.38252807912978426 1105 48-49 0.32307065572668847 1105 50-51 0.33011212979975824 1105 52-53 0.30454809354731793 1105 54-55 0.280809301641618 1105 56-57 0.14359531695145478 1105 58-59 0.3592511065036703 1105 60-61 0.309601971316269 1105 62-63 0.46438535348305265 1105 64-65 0.252669555738251 1105 66-67 0.2833938937629483 1105 68-69 0.275526753796143 1105 70-71 0.280583491746917 1105 72-73 0.29745195915668177 1105 74-75 0.23914117238857102 1105 76-77 0.24586504277844057 1105 78-79 0.3258070896272045 1105 80-81 0.3306068696710369 1105 82-83 0.3201430497633737 1105 84-85 0.3072196178324802 1105 86-87 0.34189987371582475 1105 88-89 0.3069489619848724 1105 90-91 0.23864243733089552 1105 92-93 0.2974554269689449 1105 94-95 0.26068212200932095 1105 96-97 0.23900813870511683 1105 98-99 0.2190203413387266 1105 100 0.18415302864203298 1106 1 0.5355075945348098 1106 2 3.8855325424646594 1106 3 2.199986914617927 1106 4 0.9592094665568709 1106 5 0.32677634550321955 1106 6 0.21289128548926328 1106 7 0.14193899316974523 1106 8 0.12191114446768125 1106 9 0.17939696076005163 1106 10-11 0.20515139520511383 1106 12-13 0.1608023867785633 1106 14-15 0.24382253156430522 1106 16-17 0.206389649105013 1106 18-19 0.26919839584586214 1106 20-21 0.19812576473991328 1106 22-23 0.13857520556558 1106 24-25 0.20415530684618943 1106 26-27 0.19576568832836472 1106 28-29 0.2336018806765523 1106 30-31 0.17881738282285653 1106 32-33 0.21447910097504774 1106 34-35 0.30090066947444427 1106 36-37 0.21881301391432828 1106 38-39 0.2642754022474705 1106 40-41 0.25613110689489105 1106 42-43 0.24022440024073433 1106 44-45 0.2130195443594829 1106 46-47 0.1987523740250765 1106 48-49 0.15990483642476505 1106 50-51 0.1286691976716483 1106 52-53 0.15350913536635602 1106 54-55 0.08504007625105459 1106 56-57 0.1875220159169544 1106 58-59 0.08482775412210941 1106 60-61 0.14250465711506166 1106 62-63 0.23366971755730503 1106 64-65 0.2445186906651955 1106 66-67 0.1092057741278154 1106 68-69 0.06613676209904895 1106 70-71 0.03667215407924118 1106 72-73 0.07983131469983817 1106 74-75 0.11238634466438668 1106 76-77 0.13844940606131928 1106 78-79 0.17770190083663806 1106 80-81 0.11578047456001883 1106 82-83 0.1687439629204448 1106 84-85 0.17267573400855696 1106 86-87 0.19698369902805268 1106 88-89 0.29056045188875146 1106 90-91 0.17502276051655485 1106 92-93 0.26325605033192545 1106 94-95 0.20406560342144076 1106 96-97 0.15340879712698552 1106 98-99 0.26643693854348527 1106 100 0.2657383232510213 1107 1 0.482762112552539 1107 2 3.6582021021451183 1107 3 2.1407663660317553 1107 4 0.9675053498705566 1107 5 0.4120527300751178 1107 6 0.32188191204189565 1107 7 0.20763591383872892 1107 8 0.24058309382728993 1107 9 0.23810010453820496 1107 10-11 0.2065263364721588 1107 12-13 0.19152786643054753 1107 14-15 0.2576504905492101 1107 16-17 0.20067110752948025 1107 18-19 0.21685804748533855 1107 20-21 0.26483176621140814 1107 22-23 0.2202724565207319 1107 24-25 0.3039169590341899 1107 26-27 0.23156224641146395 1107 28-29 0.12988848943289355 1107 30-31 0.14572686494046394 1107 32-33 0.25862370713327465 1107 34-35 0.3657575688597703 1107 36-37 0.19485007820122746 1107 38-39 0.14388886952438895 1107 40-41 0.1789996596377108 1107 42-43 0.2107605027704409 1107 44-45 0.32742067385984086 1107 46-47 0.2806086214324779 1107 48-49 0.14278304535991992 1107 50-51 0.15528736869787707 1107 52-53 0.11535102032995326 1107 54-55 0.10372572226022214 1107 56-57 0.1351388043322217 1107 58-59 0.21703349455221854 1107 60-61 0.273980199674817 1107 62-63 0.24127456736793818 1107 64-65 0.17602848028835894 1107 66-67 0.19643221782158093 1107 68-69 0.12942643405286702 1107 70-71 0.19274297369915416 1107 72-73 0.14937493776717048 1107 74-75 0.20277895547970104 1107 76-77 0.0767693896035837 1107 78-79 0.06165554153990627 1107 80-81 0.23293230251879748 1107 82-83 0.23793516107701862 1107 84-85 0.21444338944132113 1107 86-87 0.15566186464306497 1107 88-89 0.17472237087312692 1107 90-91 0.20746206200266215 1107 92-93 0.23208959078311153 1107 94-95 0.11353983375731502 1107 96-97 0.11609500057564048 1107 98-99 0.1487680321464282 1107 100 0.19448299975280037 1108 1 0.4803717080799217 1108 2 3.6901587924286012 1108 3 2.178050280217139 1108 4 0.9717195698583794 1108 5 0.310899646430812 1108 6 0.1699804856434639 1108 7 0.14534943766256703 1108 8 0.08624597876304563 1108 9 0.06445099801572951 1108 10-11 0.06728618806449305 1108 12-13 0.11106686905156238 1108 14-15 0.11037051995173641 1108 16-17 0.07715729021686002 1108 18-19 0.15672133643766273 1108 20-21 0.06768515327053848 1108 22-23 0.018023162744896126 1108 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0.7811182846690912 2203 44-45 0.9609323235005149 2203 46-47 0.9666345356998534 2203 48-49 0.9806210138430416 2203 50-51 0.965620607335218 2203 52-53 0.9886017126379514 2203 54-55 0.9124024276406502 2203 56-57 0.9925802901874263 2203 58-59 1.0402805106116375 2203 60-61 0.9916435494787876 2203 62-63 1.0146807102385367 2203 64-65 0.9213924257697457 2203 66-67 0.9268447899034271 2203 68-69 0.9217620658498262 2203 70-71 0.8937835006635808 2203 72-73 0.9471895722401129 2203 74-75 0.8641290444762006 2203 76-77 0.8314236219981836 2203 78-79 0.7551166372872444 2203 80-81 0.7210152186630907 2203 82-83 0.8315378002167648 2203 84-85 0.8771355829352672 2203 86-87 0.8968379215958251 2203 88-89 0.8144910503615108 2203 90-91 0.9110456065508146 2203 92-93 0.9542817346868162 2203 94-95 0.9276753615555435 2203 96-97 0.8956823408895715 2203 98-99 0.9534087562829328 2203 100 0.7765509943951798 2204 1 0.4688676540022563 2204 2 4.029279361830323 2204 3 2.286794006139761 2204 4 1.0697472210003056 2204 5 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2210 76-77 0.1166675472755827 2210 78-79 0.2237481275255533 2210 80-81 0.2029970530186347 2210 82-83 0.41848943652932746 2210 84-85 0.358714776717747 2210 86-87 0.3794541489472891 2210 88-89 0.3788597005792127 2210 90-91 0.3122453350025225 2210 92-93 0.39149555555857773 2210 94-95 0.3060837608251745 2210 96-97 0.21497748579335507 2210 98-99 0.295786676597551 2210 100 0.23003898540599366 2211 1 0.4702709808489516 2211 2 3.967422997120625 2211 3 2.257693460505955 2211 4 1.051035709792508 2211 5 0.4327086202912156 2211 6 0.29723517625270546 2211 7 0.25647356953718514 2211 8 0.20108573997344337 2211 9 0.2049165611967254 2211 10-11 0.190976265292214 2211 12-13 0.15886900497702072 2211 14-15 0.08238653467720525 2211 16-17 0.13316823224419494 2211 18-19 0.2004963469641794 2211 20-21 0.09671289167597052 2211 22-23 0.039223157641131934 2211 24-25 0.0809017389445259 2211 26-27 0.09444394906177678 2211 28-29 0.1752992644121747 2211 30-31 0.12251016028606898 2211 32-33 0.1180580361053103 2211 34-35 0.20489083152364884 2211 36-37 0.09339527141255388 2211 38-39 0.04323638337174884 2211 40-41 0.0021789292594434073 2211 42-43 0.15523024474947533 2211 44-45 0.14862347179196433 2211 46-47 0.19632632754935742 2211 48-49 0.02999466564326525 2211 50-51 -0.025031191072152126 2211 52-53 0.021489861704303337 2211 54-55 0.10125572810617456 2211 56-57 -0.10677709150500192 2211 58-59 -0.08456778223852979 2211 60-61 -0.05290129963378831 2211 62-63 0.11548644854816814 2211 64-65 0.04680485092385567 2211 66-67 0.0599904527704922 2211 68-69 0.11861818737315133 2211 70-71 0.010509386597554737 2211 72-73 0.15207112540249312 2211 74-75 0.019223037466353787 2211 76-77 0.0310180241753244 2211 78-79 0.054843272302875334 2211 80-81 -0.00858002815568426 2211 82-83 -0.3835769827329756 2211 84-85 -0.0797416568935958 2211 86-87 -0.019995231562397464 2211 88-89 0.04844927886945527 2211 90-91 0.025875113483898815 2211 92-93 0.03913933671069714 2211 94-95 0.10980728461661471 2211 96-97 0.0267055529100233 2211 98-99 0.055042039602007264 2211 100 -0.08443768016054776 2212 1 0.4702336149205877 2212 2 4.013384472929889 2212 3 2.328812382009666 2212 4 1.0063769128190927 2212 5 0.4169402799294488 2212 6 0.4174740121029643 2212 7 0.39563519533116676 2212 8 0.35113208512162686 2212 9 0.42078189015772693 2212 10-11 0.34719429768220067 2212 12-13 0.35063477547467414 2212 14-15 0.39781789786279376 2212 16-17 0.5043185850876455 2212 18-19 0.4423036025971143 2212 20-21 0.2970107933715056 2212 22-23 0.39481597420014225 2212 24-25 0.2838009686528338 2212 26-27 0.2415391592382079 2212 28-29 0.23867273369127417 2212 30-31 0.3114513433841637 2212 32-33 0.4141424368640898 2212 34-35 0.46904156909776873 2212 36-37 0.3419252192679494 2212 38-39 0.40585399446293735 2212 40-41 0.3722645566626852 2212 42-43 0.32705929798802913 2212 44-45 0.4848259358592131 2212 46-47 0.5266859896701845 2212 48-49 0.3645267343203713 2212 50-51 0.28440794715719875 2212 52-53 0.3338474498939945 2212 54-55 0.19258456960447035 2212 56-57 0.32945941707475157 2212 58-59 0.29738523144977336 2212 60-61 0.31641156943616267 2212 62-63 0.3544112769294827 2212 64-65 0.3851582166822354 2212 66-67 0.26539055394341915 2212 68-69 0.3541699877026545 2212 70-71 0.3348204492406026 2212 72-73 0.3814846561668688 2212 74-75 0.40735132031552723 2212 76-77 0.5283436331544777 2212 78-79 0.47979030467479333 2212 80-81 0.4099202418721646 2212 82-83 0.5224225118908095 2212 84-85 0.5653777585893138 2212 86-87 0.30928045230329104 2212 88-89 0.591834093063099 2212 90-91 0.38874231920767244 2212 92-93 0.5354233399090234 2212 94-95 0.5572996889130337 2212 96-97 0.38717987388807273 2212 98-99 0.3890618531500891 2212 100 0.509080772176894 2213 1 -0.5724297533033287 2213 2 -25.801512940094195 2213 3 -14.732655969133155 2213 4 -6.096210193475368 2213 5 -2.0316589292891507 2213 6 -1.4385141243507675 2213 7 -1.2513744979079178 2213 8 -1.1038912124988585 2213 9 -1.1322493038032704 2213 10-11 -1.1874950886734226 2213 12-13 -1.1961938458783052 2213 14-15 -1.3774348374465575 2213 16-17 -1.4595277296274887 2213 18-19 -1.4125703706921797 2213 20-21 -1.5248224499560976 2213 22-23 -1.496467098994458 2213 24-25 -1.3427082136266293 2213 26-27 -1.4608726249326551 2213 28-29 -1.5686310162230015 2213 30-31 -1.4515180217247163 2213 32-33 -1.2370866824365692 2213 34-35 -1.3544660155039239 2213 36-37 -1.4543254270914119 2213 38-39 -1.5874869721907885 2213 40-41 -1.433894064812847 2213 42-43 -1.623098735734004 2213 44-45 -1.4189567429355847 2213 46-47 -1.3969825882818583 2213 48-49 -1.5268415422711001 2213 50-51 -1.5716464223885112 2213 52-53 -1.72687317136198 2213 54-55 -1.5410525319832757 2213 56-57 -1.4534034092373105 2213 58-59 -1.514289641775136 2213 60-61 -1.578033612380196 2213 62-63 -1.4547181203676374 2213 64-65 -1.5182891896794288 2213 66-67 -1.5173856347906423 2213 68-69 -1.590445848629635 2213 70-71 -1.5213684073806704 2213 72-73 -1.4897762054735075 2213 74-75 -1.5993575182298443 2213 76-77 -1.5547260933419693 2213 78-79 -1.4090881031025333 2213 80-81 -1.360329678530782 2213 82-83 -1.4690277477090135 2213 84-85 -1.4601198957422632 2213 86-87 -1.3141265962014934 2213 88-89 -1.4212644787271813 2213 90-91 -1.4234246737134697 2213 92-93 -1.4387833036873197 2213 94-95 -1.5241254229726522 2213 96-97 -1.5105613169151297 2213 98-99 -1.49312825094799 2213 100 -1.4003175731953057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 35.0 5 53.0 6 56.0 7 52.0 8 67.0 9 125.0 10 551.0 11 2395.0 12 6959.0 13 12680.0 14 16400.0 15 18505.0 16 20227.0 17 20688.0 18 21454.0 19 22835.0 20 25115.0 21 28094.0 22 30921.0 23 32829.0 24 34763.0 25 36636.0 26 40679.0 27 46525.0 28 54648.0 29 65313.0 30 79161.0 31 96248.0 32 121685.0 33 162550.0 34 223113.0 35 317043.0 36 484503.0 37 829883.0 38 852853.0 39 7010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.40021807640074 21.056098086499286 10.63013633039663 29.91354750670335 2 12.680630542813843 22.972550751923542 36.787337079392636 27.55948162586998 3 15.067188842531115 27.861431261060098 29.04155230602235 28.029827590386436 4 10.301769186842078 19.038395233323634 38.306316061673726 32.35351951816056 5 11.47459554199073 38.78202032313472 35.621255380677 14.122128754197563 6 29.341178100229364 40.14033588013374 17.96764949932209 12.550836520314801 7 25.2321369967985 33.410806636658116 24.048374380891666 17.308681985651724 8 23.971351237013813 37.189473358024664 21.391615278801343 17.447560126160187 9 24.426340160836816 16.734600393671613 22.38124172272423 36.457817722767345 10-11 22.621975733654384 28.05352952779032 29.491170926085402 19.83332381246989 12-13 23.562528570645227 25.592763841193488 29.78359668737473 21.061110900786552 14-15 21.29844139015016 26.891331683595855 26.8508180401178 24.959408886136185 16-17 20.46558077764656 30.518635813860374 27.849542588582455 21.166240819910605 18-19 20.428115680493537 29.501511058328173 29.796380949968977 20.273992311209312 20-21 21.79223229774207 28.792596266504876 28.78421771097324 20.630953724779808 22-23 20.71016047909612 28.952619777397793 29.014772525521828 21.322447217984262 24-25 21.06089370830838 28.638332596519273 28.780522892826287 21.52025080234606 26-27 21.05155617286212 28.57227712850441 28.887994237354615 21.48817246127886 28-29 20.624080341079974 28.544475257639196 29.411049215323164 21.42039518595766 30-31 21.531576651708104 28.91033725065056 28.608961290842284 20.94912480679906 32-33 20.29089734716671 28.95811591585501 29.441764168683775 21.30922256829451 34-35 20.525452356065646 28.404826945042533 29.532439361842965 21.537281337048856 36-37 21.314425342784098 28.89561480666219 28.712274961552236 21.077684889001482 38-39 21.026024007138165 28.816175356487484 29.01345813902591 21.14434249734844 40-41 21.1641574145219 28.64041115954453 28.79510778324964 21.40032364268393 42-43 20.836656085488414 28.76878210768505 29.06888369938715 21.325678107439387 44-45 21.380213008704516 28.179281585536625 28.881525844514716 21.558979561244143 46-47 21.204739953406477 28.920768383644425 28.859482385191793 21.015009277757304 48-49 21.08233501415291 28.689263818267268 28.959053478512537 21.26934768906728 50-51 21.33380659144377 28.637777716412877 28.519710145122758 21.508705547020597 52-53 20.751665397465253 28.940297870099062 28.551373963854093 21.756662768581585 54-55 21.210120211866005 28.759584350365593 28.30476213568692 21.72553330208148 56-57 21.400180625886883 28.56126295344203 28.338477556795798 21.700078863875294 58-59 20.86235690701901 28.197302876871174 29.09057456203503 21.849765654074783 60-61 21.589196996843135 28.59200151283852 28.240339289596246 21.5784622007221 62-63 20.720644998609917 28.885794427167184 28.70912454930054 21.68443602492236 64-65 20.768619751449737 28.230380912753635 28.92291159494882 22.078087740847806 66-67 21.209252091696552 28.554321609272137 28.450831223250166 21.785595075781146 68-69 21.343244536453994 28.447147554269048 28.562175956410172 21.64743195286678 70-71 21.425745401837045 28.39540665293072 28.22914737224257 21.94970057298967 72-73 21.0461565236742 28.66416717102453 28.69287280038569 21.596803504915588 74-75 21.549247849265953 28.001261397570314 28.404251249273926 22.045239503889803 76-77 21.24329704996114 28.561928456068653 28.533399245956005 21.661375248014203 78-79 21.220046536843913 28.52022938421182 28.69934572295425 21.56037835599002 80-81 21.351413892274156 28.29092410787886 28.40853744608266 21.949124553764328 82-83 20.68302122720351 28.68635549525248 28.6105411680282 22.02008210951581 84-85 21.000646100372776 28.145193582958573 28.39199665218452 22.46216366448413 86-87 21.230097667311586 28.421558057267 28.253106693271572 22.095237582149842 88-89 20.8564742110642 28.0799971770329 28.769509584889153 22.294019027013746 90-91 21.25970009423824 28.089581953666354 28.426870545586485 22.223847406508916 92-93 20.569480800383136 28.58443667135171 28.607318690636713 22.238763837628436 94-95 20.54532400783975 27.95384174269454 28.84046664099745 22.660367608468253 96-97 20.960634998618232 28.2085002631552 28.529404159522155 22.301460578704415 98-99 20.811160237512606 28.04667561805667 28.582762424093872 22.55940172033685 100 20.924461106713057 27.914812473980184 28.480426627896694 22.680299791410064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18.0 1 13.5 2 15.0 3 20.5 4 21.0 5 30.0 6 60.5 7 110.0 8 169.0 9 207.0 10 192.5 11 193.0 12 224.0 13 303.0 14 371.5 15 426.5 16 539.5 17 658.0 18 839.5 19 1109.5 20 1585.5 21 2273.5 22 3324.5 23 4955.0 24 7301.5 25 10831.5 26 16109.0 27 23745.0 28 34098.0 29 46607.0 30 60333.0 31 75209.5 32 90370.0 33 104772.5 34 118077.0 35 130004.5 36 140238.5 37 148665.5 38 155580.5 39 160126.0 40 162369.0 41 161849.0 42 157761.0 43 151839.0 44 144913.5 45 136848.5 46 130084.5 47 125475.5 48 119459.0 49 116994.5 50 129795.5 51 123237.5 52 96203.0 53 83301.0 54 75939.0 55 69280.0 56 63139.5 57 56515.5 58 50105.0 59 44096.5 60 37333.0 61 30391.5 62 24515.0 63 19567.0 64 15308.5 65 12004.5 66 9198.0 67 6816.5 68 5142.5 69 3831.0 70 2702.5 71 1996.5 72 1508.0 73 1088.0 74 804.0 75 580.5 76 355.5 77 232.5 78 168.5 79 95.5 80 49.5 81 19.0 82 13.5 83 9.5 84 5.5 85 2.5 86 0.5 87 0.0 88 1.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.9937322735389895 2 6.465508650108951 3 0.0 4 0.006195002518403197 5 0.17108980868216134 6 0.05389652191010782 7 0.034584274928824814 8 0.04080621224078628 9 0.039190124627289796 10-11 0.0409947557956942 12-13 0.03971535310167615 14-15 0.04240883245750363 16-17 0.03342607880581899 18-19 0.02782364174569784 20-21 0.02189798716287739 22-23 0.022813770143858735 24-25 0.020012551613798155 26-27 0.013090309669321539 28-29 0.018746616316559245 30-31 0.02319085725367458 32-33 0.001467946248925975 34-35 0.003649664527146232 36-37 0.004457708333894475 38-39 0.006262339502298884 40-41 0.006531687437881632 42-43 0.007016513721930579 44-45 0.007716818354445722 46-47 0.015245093153983522 48-49 0.02395849887008541 50-51 0.021884519766098252 52-53 0.027433087239102854 54-55 0.02397196626686455 56-57 0.01555484327990368 58-59 0.014261973189106492 60-61 0.011932113546315725 62-63 0.0178308333355779 64-65 0.018975562061804577 66-67 0.019689334091098857 68-69 0.026342228099992728 70-71 0.026867456574379084 72-73 0.023015781095545793 74-75 0.024618401312263144 76-77 0.01845033358741822 78-79 0.015016147408738184 80-81 0.013629005540487035 82-83 0.008376720796623454 84-85 0.010733515232972496 86-87 0.008686470922543614 88-89 0.0071107854993845395 90-91 0.007285861657513326 92-93 0.003986349446624666 94-95 0.0013871418682511508 96-97 8.619133938647928E-4 98-99 6.733698389568693E-4 100 0.10660791290365156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3712670.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.44780033840557 #Duplication Level Percentage of deduplicated Percentage of total 1 89.24635024957024 75.3665796680712 2 8.363274948721953 14.125203460897213 3 1.500575704685741 3.801609525058912 4 0.43471977804552053 1.4684451607817643 5 0.1704588948517386 0.7197439359172446 6 0.08229291736831942 0.41696735131108487 7 0.05094879496574321 0.3011759565324612 8 0.02838277325934031 0.19174902154040058 9 0.023572694686418975 0.17915959928852132 >10 0.0934244896200692 1.291391028172251 >50 0.0031208658735240153 0.17921019077488334 >100 0.0018216183905211854 0.30513831170695027 >500 5.441390833140478E-4 0.3511223591371294 >1k 4.4811451780927473E-4 0.7692525701210173 >5k 3.200817984351919E-5 0.22794570869779304 >10k+ 3.200817984351919E-5 0.3053061519911742 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 11295 0.30422849324071355 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8433 0.22714111407693116 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.6934793558274773E-5 0.0 8 0.0 0.0 0.0 5.3869587116549546E-5 0.0 9 0.0 0.0 0.0 1.3467396779137387E-4 0.0 10-11 0.0 0.0 0.0 1.7507615812878603E-4 0.0 12-13 0.0 0.0 0.0 1.885435549079234E-4 0.0 14-15 0.0 2.6934793558274773E-5 0.0 2.0201095168706078E-4 0.0 16-17 0.0 2.6934793558274773E-5 0.0 2.6934793558274774E-4 0.0 18-19 0.0 2.6934793558274773E-5 0.0 2.962827291410225E-4 0.0 20-21 0.0 4.0402190337412156E-5 0.0 3.2321752269929725E-4 0.0 22-23 0.0 5.3869587116549546E-5 0.0 3.6361971303670946E-4 0.0 24-25 0.0 5.3869587116549546E-5 0.0 4.578914904906712E-4 0.0 26-27 0.0 5.3869587116549546E-5 0.0 6.329676486194571E-4 0.0 28-29 2.6934793558274773E-5 8.080438067482431E-5 0.0 9.965873616561666E-4 0.0 30-31 5.3869587116549546E-5 8.080438067482431E-5 0.0 0.002855088117177126 0.0 32-33 5.3869587116549546E-5 8.080438067482431E-5 0.0 0.008996221048463773 0.0 34-35 5.3869587116549546E-5 8.080438067482431E-5 0.0 0.018975562061804577 0.0 36-37 5.3869587116549546E-5 1.0773917423309909E-4 0.0 0.0342206552157881 0.0 38-39 5.3869587116549546E-5 1.885435549079234E-4 0.0 0.05734417548556699 0.0 40-41 5.3869587116549546E-5 1.885435549079234E-4 0.0 0.08784782919031317 0.0 42-43 5.3869587116549546E-5 1.885435549079234E-4 0.0 0.1181494719433723 0.0 44-45 5.3869587116549546E-5 1.885435549079234E-4 0.0 0.1558312481313987 0.0 46-47 5.3869587116549546E-5 2.289457452453356E-4 0.0 0.20122984267387084 0.0 48-49 8.080438067482431E-5 2.6934793558274774E-4 0.0 0.24885055768490064 0.0 50-51 8.080438067482431E-5 2.6934793558274774E-4 0.0 0.29909741506786225 0.0 52-53 8.080438067482431E-5 2.6934793558274774E-4 0.0 0.3531420783425405 0.0 54-55 8.080438067482431E-5 2.962827291410225E-4 0.0 0.41348948330985513 0.0 56-57 8.080438067482431E-5 2.962827291410225E-4 0.0 0.48413944681320986 0.0 58-59 8.080438067482431E-5 3.0975012592015985E-4 0.0 0.5643108598394148 0.0 60-61 8.080438067482431E-5 3.2321752269929725E-4 0.0 0.6497345576094833 0.0 62-63 8.080438067482431E-5 3.2321752269929725E-4 0.0 0.7357104186474963 0.0 64-65 8.080438067482431E-5 3.3668491947843465E-4 0.0 0.8374835361074375 0.0 66-67 8.080438067482431E-5 3.5015231625757206E-4 0.0 0.9521045500946759 0.0 68-69 8.080438067482431E-5 4.040219033741216E-4 0.0 1.0823342769489344 0.0 70-71 8.080438067482431E-5 4.1748930015325896E-4 0.0 1.2346101323306407 0.0 72-73 8.080438067482431E-5 4.3095669693239637E-4 0.0 1.3868186507284515 0.0 74-75 8.080438067482431E-5 4.4442409371153377E-4 0.0 1.5457878023093892 0.0 76-77 8.080438067482431E-5 4.578914904906711E-4 0.0 1.725550614517315 0.0 78-79 8.080438067482431E-5 4.578914904906711E-4 0.0 1.927467294426922 0.0 80-81 8.080438067482431E-5 4.578914904906711E-4 0.0 2.1498813522343756 0.0 82-83 8.080438067482431E-5 4.578914904906711E-4 0.0 2.390449460900107 0.0 84-85 8.080438067482431E-5 4.578914904906711E-4 0.0 2.6421147045118474 0.0 86-87 8.080438067482431E-5 4.578914904906711E-4 0.0 2.9130787277080916 0.0 88 8.080438067482431E-5 4.8482628404894593E-4 0.0 3.137876514745453 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 310 6.348273E-9 18.199926 5 GTCGTAA 175 0.0014777384 17.249743 1 GTCCTAT 1170 0.0 16.340567 1 GAGTACT 4140 0.0 16.099916 12-13 TATACTG 2160 0.0 15.23682 5 GTATATA 2955 0.0 14.812594 1 GTACTAG 850 0.0 14.797573 1 TATACAG 2395 0.0 13.741769 5 TAGCCCG 240 0.0011911322 13.7131405 5 CTATACA 2060 0.0 13.465884 4 TACCGTA 280 2.526491E-4 13.411378 7 GTACATG 21235 0.0 13.410148 1 CCTATAC 1160 0.0 13.374836 3 AGTACTT 5155 0.0 13.203072 12-13 ACTGTCG 325 6.0985607E-5 12.99872 8 GTATAGG 1085 0.0 12.983677 1 ACATGGG 20385 0.0 12.938521 3 TACATGG 21355 0.0 12.804644 2 CATGGGT 3055 0.0 12.773708 4 CGCACTG 445 5.3502845E-7 12.6786 5 >>END_MODULE