FastQCFastQC Report
Wed 25 May 2016
SRR1295123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3649754
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97350.017.653681
GTATACG3658.432489E-716.6966441
TACATGG102800.016.119822
ACATGGG107300.015.3275873
ATAGCGC2350.00581627514.0438343
TAGCGCT3553.15148E-513.9446854
GGTACCG2550.001222128413.6664295
CTAGACT12600.013.532744
TATACCG3354.665379E-513.3750345
GTATTAA18750.013.3261491
CATGGGG44250.013.300414
GTATAGA13350.013.2385011
GTATATG20550.013.0485711
GTCTAAG11250.013.00112151
GAACCGT3456.1705665E-512.9823076
GTAGACG2550.00998214812.9423573
TAAGGGT12750.012.9421264
GTATTAC11800.012.9116011
GTCTCGC5252.5818754E-612.7689581
CTATACT14650.012.7654414
Produced by FastQC (version 0.11.5)