##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295123_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3649754 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.278785090721183 33.0 27.0 33.0 6.0 33.0 2 22.298435182206802 27.0 6.0 33.0 2.0 33.0 3 24.708679543881587 27.0 15.0 33.0 15.0 33.0 4 25.289583626732103 33.0 15.0 37.0 2.0 37.0 5 27.021902572063762 33.0 15.0 37.0 2.0 37.0 6 27.46913298814112 33.0 15.0 37.0 6.0 37.0 7 27.32365688208027 33.0 15.0 37.0 6.0 37.0 8 28.0309755123222 33.0 22.0 37.0 6.0 37.0 9 29.114594024693172 33.0 27.0 37.0 15.0 37.0 10-11 28.60546861514502 33.0 24.5 37.0 10.5 37.0 12-13 27.48885445430021 33.0 15.0 37.0 4.0 37.0 14-15 29.178405174704928 33.0 22.0 37.0 10.5 40.0 16-17 29.54728606366347 33.0 27.0 37.0 6.0 40.0 18-19 29.632515780515618 33.0 22.0 40.0 10.5 40.0 20-21 29.15304360239074 33.0 22.0 38.5 6.0 40.0 22-23 29.868385513105814 33.0 27.0 37.0 15.0 40.0 24-25 29.96922025429659 33.0 27.0 37.0 15.0 40.0 26-27 28.513388299594986 33.0 18.5 37.0 4.0 40.0 28-29 29.226499786012976 33.0 22.0 37.0 6.0 40.0 30-31 29.911088528158338 33.0 27.0 37.0 15.0 40.0 32-33 28.693325221371083 33.0 22.0 37.0 6.0 40.0 34-35 28.397396098476776 33.0 22.0 37.0 6.0 40.0 36-37 28.690735594782552 33.0 22.0 37.0 6.0 40.0 38-39 28.79730113317226 33.0 22.0 37.0 6.0 40.0 40-41 27.925067552498057 33.0 18.5 37.0 6.0 40.0 42-43 28.55808281873244 33.0 22.0 37.0 6.0 40.0 44-45 28.354093042983173 33.0 22.0 37.0 6.0 40.0 46-47 27.995701628109728 33.0 22.0 37.0 6.0 40.0 48-49 27.90423751847385 33.0 18.5 37.0 6.0 40.0 50-51 27.825090814339816 33.0 22.0 37.0 6.0 40.0 52-53 28.05707179168788 33.0 22.0 37.0 6.0 40.0 54-55 28.022444526398218 33.0 22.0 37.0 6.0 40.0 56-57 28.00352736102214 33.0 22.0 37.0 6.0 40.0 58-59 27.86537188533803 33.0 22.0 37.0 6.0 40.0 60-61 27.869040488756227 33.0 22.0 37.0 6.0 40.0 62-63 27.79195323849224 33.0 22.0 37.0 6.0 40.0 64-65 27.3344721315464 33.0 22.0 37.0 6.0 40.0 66-67 27.576280757552425 33.0 22.0 37.0 6.0 38.5 68-69 27.18729591090249 33.0 22.0 37.0 6.0 37.0 70-71 27.32945521807771 33.0 22.0 37.0 6.0 37.0 72-73 27.348901323212466 33.0 22.0 37.0 10.5 37.0 74-75 27.44224309364412 30.0 22.0 37.0 10.5 37.0 76-77 27.21158220526644 27.0 22.0 37.0 6.0 37.0 78-79 26.747308037747203 27.0 22.0 33.0 6.0 37.0 80-81 26.58728190995886 27.0 22.0 33.0 6.0 37.0 82-83 26.690011710378286 27.0 22.0 33.0 10.5 37.0 84-85 26.61599233811375 27.0 22.0 33.0 6.0 37.0 86-87 26.427871029115934 27.0 22.0 33.0 10.5 37.0 88-89 26.3424618481136 27.0 22.0 33.0 6.0 37.0 90-91 26.501532980030984 27.0 22.0 33.0 15.0 37.0 92-93 26.442527907360333 27.0 22.0 33.0 10.5 37.0 94-95 26.30021982303465 27.0 22.0 33.0 10.5 37.0 96-97 26.37497513531049 27.0 22.0 33.0 10.5 37.0 98-99 26.417070301176462 27.0 22.0 33.0 10.5 37.0 100 26.1016994570045 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.188061626846007 1101 2 11.177725686030389 1101 3 8.390449637571965 1101 4 12.812967968835874 1101 5 10.721202098558312 1101 6 9.840366264685251 1101 7 9.327591527567897 1101 8 8.845433924516005 1101 9 7.264453594554656 1101 10-11 7.7413079242124 1101 12-13 9.022662949685703 1101 14-15 7.830319173090505 1101 16-17 8.091373216704124 1101 18-19 8.05179594340471 1101 20-21 7.118061168606051 1101 22-23 7.242955939347421 1101 24-25 7.244442221006693 1101 26-27 8.401508924561732 1101 28-29 8.14118922598707 1101 30-31 5.473544965247097 1101 32-33 6.821198189017078 1101 34-35 8.589162154803812 1101 36-37 7.967374363216138 1101 38-39 8.053216539373054 1101 40-41 8.810599085798103 1101 42-43 8.405695669156792 1101 44-45 8.265879372029474 1101 46-47 7.192948445887602 1101 48-49 5.345837195040179 1101 50-51 6.689378193253457 1101 52-53 5.785598001534588 1101 54-55 3.965366298240994 1101 56-57 5.991293473158308 1101 58-59 6.087969945626824 1101 60-61 6.57471400601808 1101 62-63 6.0475460540429005 1101 64-65 6.526797250220369 1101 66-67 5.6868960151265995 1101 68-69 6.637599833059717 1101 70-71 6.148471408273064 1101 72-73 5.5141581110105875 1101 74-75 4.863783646398296 1101 76-77 4.846887203057339 1101 78-79 4.959652397877722 1101 80-81 4.800098653393068 1101 82-83 4.2792125956724725 1101 84-85 4.104222002598707 1101 86-87 3.976908371770868 1101 88-89 3.9069271905568392 1101 90-91 3.7439650158257614 1101 92-93 3.6566391726921523 1101 94-95 3.50520875935678 1101 96-97 3.605999533562528 1101 98-99 3.371870844136428 1101 100 3.6317277355463027 1102 1 6.593399307811563 1102 2 10.334563254595238 1102 3 8.004657698822442 1102 4 12.433206042163395 1102 5 10.418176309907171 1102 6 9.318062781377698 1102 7 6.6656500139627965 1102 8 7.800554081533253 1102 9 6.384673179271385 1102 10-11 7.260304054604767 1102 12-13 8.792500760954482 1102 14-15 6.181196013535043 1102 16-17 7.209364204522512 1102 18-19 7.172810127358456 1102 20-21 6.847123364916914 1102 22-23 6.336415029638655 1102 24-25 6.81328383639277 1102 26-27 8.243913064159408 1102 28-29 7.435519285884844 1102 30-31 3.9264027297991397 1102 32-33 4.312561267594713 1102 34-35 5.014935558359465 1102 36-37 4.869438973976685 1102 38-39 4.416295208343481 1102 40-41 6.057958453196239 1102 42-43 6.705042703791417 1102 44-45 6.189491686092772 1102 46-47 5.216649977202795 1102 48-49 3.4521581321443584 1102 50-51 4.849132258346373 1102 52-53 5.557217524222747 1102 54-55 5.229146573835926 1102 56-57 6.106102486569959 1102 58-59 5.745793705368719 1102 60-61 5.766458985157609 1102 62-63 4.200639926299218 1102 64-65 5.954312769578536 1102 66-67 3.6841896372074316 1102 68-69 5.837867521351146 1102 70-71 5.164408377653171 1102 72-73 4.833152997665202 1102 74-75 3.9551374555522045 1102 76-77 3.071781751374182 1102 78-79 4.256417359864024 1102 80-81 4.197308934388136 1102 82-83 3.7204045111213198 1102 84-85 2.8843759563245683 1102 86-87 3.0396527797500994 1102 88-89 3.156039695374801 1102 90-91 3.0775718801255536 1102 92-93 3.0366613023946947 1102 94-95 2.783286082061011 1102 96-97 3.0088890530076817 1102 98-99 2.7848049997615725 1102 100 2.9698092546856536 1103 1 6.506764083552671 1103 2 10.8331809969123 1103 3 7.786207443358645 1103 4 11.688136085342517 1103 5 10.192007861058269 1103 6 8.787210944846855 1103 7 7.826583398762438 1103 8 7.686686125810876 1103 9 6.876897432168324 1103 10-11 7.132100980369238 1103 12-13 8.476405550134011 1103 14-15 8.211496708652984 1103 16-17 7.781890005697473 1103 18-19 6.93959817334056 1103 20-21 8.120970595766817 1103 22-23 6.576808908284239 1103 24-25 5.053198907765378 1103 26-27 5.811013066972528 1103 28-29 6.766572906304241 1103 30-31 5.820940522254343 1103 32-33 7.678765982664604 1103 34-35 6.856064814878874 1103 36-37 4.018737976148323 1103 38-39 6.126532967917434 1103 40-41 6.4551927618529525 1103 42-43 7.084494642978164 1103 44-45 5.433114037780118 1103 46-47 6.069891086237114 1103 48-49 5.289543814457982 1103 50-51 6.17279801974102 1103 52-53 5.7874672603195805 1103 54-55 4.698590207393984 1103 56-57 5.589274295947249 1103 58-59 5.173015806918752 1103 60-61 4.753073595224254 1103 62-63 2.496292937331731 1103 64-65 4.726480427986154 1103 66-67 3.8720810820409497 1103 68-69 4.923468185248041 1103 70-71 3.53350853357394 1103 72-73 4.1847048958541535 1103 74-75 3.035460932350169 1103 76-77 3.3498993712512437 1103 78-79 2.78558678777177 1103 80-81 3.8789746164619494 1103 82-83 3.231925215933007 1103 84-85 3.060047958239334 1103 86-87 2.583684071817231 1103 88-89 2.7870036186936957 1103 90-91 2.493303460284096 1103 92-93 2.0421731546329056 1103 94-95 2.5890812987383107 1103 96-97 2.763619226143838 1103 98-99 2.5549152511025355 1103 100 2.706910419641158 1104 1 6.235319136881024 1104 2 9.646863003492655 1104 3 7.394211991270559 1104 4 12.064830916365668 1104 5 9.412779352635898 1104 6 8.013944511149209 1104 7 3.797004507910465 1104 8 6.389733393998345 1104 9 6.343787593035845 1104 10-11 7.01081952072731 1104 12-13 7.268736754420345 1104 14-15 6.920076340770013 1104 16-17 7.064691495287246 1104 18-19 6.702228289509247 1104 20-21 7.319220820097026 1104 22-23 6.318086968139074 1104 24-25 5.803364717054038 1104 26-27 8.093573924090443 1104 28-29 7.083863796175287 1104 30-31 6.195165027102288 1104 32-33 7.439574054465137 1104 34-35 7.865308334383062 1104 36-37 6.415123798107231 1104 38-39 7.071894255675048 1104 40-41 7.150144170906792 1104 42-43 7.029406945191376 1104 44-45 6.522362118200874 1104 46-47 6.540677161600922 1104 48-49 5.283290621351931 1104 50-51 5.480830109644021 1104 52-53 5.7973394617653256 1104 54-55 4.753955322234333 1104 56-57 5.812468739617607 1104 58-59 3.706502316452088 1104 60-61 4.045397925624108 1104 62-63 3.68569006033157 1104 64-65 2.6449688139047183 1104 66-67 3.015569978856483 1104 68-69 4.037178291161627 1104 70-71 2.5278880166948348 1104 72-73 2.4091169927796656 1104 74-75 3.1251983127306104 1104 76-77 3.610563416552015 1104 78-79 4.084334718639049 1104 80-81 2.965495714648913 1104 82-83 2.8151917146539063 1104 84-85 2.7850386227583748 1104 86-87 0.5183585096912289 1104 88-89 0.28238492432340934 1104 90-91 2.0919553889665927 1104 92-93 2.214441899442658 1104 94-95 2.1871224733183077 1104 96-97 2.512000936775511 1104 98-99 2.32992806997191 1104 100 0.8084432034351359 1105 1 5.6716884522233535 1105 2 -2.7714867502019267 1105 3 0.4410189449365838 1105 4 8.378113560757757 1105 5 8.516088412391735 1105 6 7.891236222436422 1105 7 5.164517180145776 1105 8 5.870300253425771 1105 9 4.876965646866072 1105 10-11 4.946041195604156 1105 12-13 5.043945578579695 1105 14-15 5.744442729004465 1105 16-17 5.9660515295558305 1105 18-19 6.032905807892913 1105 20-21 6.875440031919691 1105 22-23 2.120353691920041 1105 24-25 3.9775115721978977 1105 26-27 6.862020950577197 1105 28-29 5.834924872779837 1105 30-31 5.051324447392144 1105 32-33 6.97441199263891 1105 34-35 6.13647185976323 1105 36-37 6.084610483336046 1105 38-39 4.055026796547466 1105 40-41 5.250469008488 1105 42-43 5.451032739437103 1105 44-45 6.101689418836273 1105 46-47 5.336315949349903 1105 48-49 4.989171609429324 1105 50-51 5.823215167115286 1105 52-53 5.407851170196825 1105 54-55 5.115486163956334 1105 56-57 4.97633502076275 1105 58-59 1.8611160809983858 1105 60-61 4.288466569873414 1105 62-63 -0.8339759994956601 1105 64-65 -0.48278784348087456 1105 66-67 0.7118887837397523 1105 68-69 2.744538896573207 1105 70-71 2.8959923573816333 1105 72-73 2.648388428490385 1105 74-75 2.620455148598392 1105 76-77 1.7652360802201805 1105 78-79 3.2383951884041693 1105 80-81 1.535882842513555 1105 82-83 0.9006159668135396 1105 84-85 1.3670485106715624 1105 86-87 -0.3527648571967532 1105 88-89 1.5740248186619539 1105 90-91 1.4542313938213098 1105 92-93 1.7174064885766605 1105 94-95 1.019667807793926 1105 96-97 1.8688600072402792 1105 98-99 1.683786854865179 1105 100 2.0001866174424165 1106 1 5.948705969974959 1106 2 10.141008162725615 1106 3 6.661134140553504 1106 4 11.533301767253416 1106 5 9.691887917958663 1106 6 9.14717989715534 1106 7 6.381061554602901 1106 8 7.097556332097728 1106 9 6.696335548404644 1106 10-11 5.429000565839772 1106 12-13 7.67284698352281 1106 14-15 7.600339784880266 1106 16-17 7.488846017902642 1106 18-19 7.034311662126267 1106 20-21 8.03792909851023 1106 22-23 5.974289415620635 1106 24-25 4.964744538857076 1106 26-27 7.441191514801865 1106 28-29 5.962222027663735 1106 30-31 5.117176524436243 1106 32-33 7.124896251566344 1106 34-35 6.494230556379339 1106 36-37 5.7230052766034945 1106 38-39 6.814806199826279 1106 40-41 6.384528704903666 1106 42-43 5.977815416664633 1106 44-45 4.229361346700067 1106 46-47 5.836586019748911 1106 48-49 5.647087853438723 1106 50-51 6.27893979401269 1106 52-53 5.387764902559113 1106 54-55 5.1927524926996576 1106 56-57 4.789341677994287 1106 58-59 4.824433732987963 1106 60-61 4.595072702908713 1106 62-63 4.676486893727446 1106 64-65 4.90276813557557 1106 66-67 2.900033095633482 1106 68-69 3.1221951076608967 1106 70-71 2.7050615446041206 1106 72-73 2.552459301280166 1106 74-75 2.5818508742427007 1106 76-77 3.268953287584438 1106 78-79 3.870302369831947 1106 80-81 3.4841504285989693 1106 82-83 1.9290787446284376 1106 84-85 2.5070979691640893 1106 86-87 3.15172695185732 1106 88-89 3.1762625231318964 1106 90-91 3.0568531342986134 1106 92-93 2.7971565994292824 1106 94-95 1.3887471387907482 1106 96-97 1.2801721378580773 1106 98-99 1.8015428636847979 1106 100 2.7502300473744477 1107 1 5.974397515676692 1107 2 10.20879536473894 1107 3 6.020520245825729 1107 4 11.1348939894994 1107 5 9.187709015383309 1107 6 7.880718582030486 1107 7 3.486935836386209 1107 8 6.139446548432389 1107 9 5.54132064526685 1107 10-11 4.486869038684446 1107 12-13 5.9587474556258755 1107 14-15 5.940857131870477 1107 16-17 6.856021948589895 1107 18-19 6.095896867826081 1107 20-21 5.26517811926038 1107 22-23 5.594011450525141 1107 24-25 4.504914429477921 1107 26-27 7.021697541056788 1107 28-29 6.307266396862026 1107 30-31 4.917034047660987 1107 32-33 4.700859118805514 1107 34-35 6.476247122045297 1107 36-37 6.373258861698076 1107 38-39 6.220658999679451 1107 40-41 7.5354023866287 1107 42-43 5.960701707884695 1107 44-45 4.101624039259079 1107 46-47 3.415472629959641 1107 48-49 3.9905443701261945 1107 50-51 4.625929413238648 1107 52-53 3.680050650152907 1107 54-55 5.061922739297856 1107 56-57 5.098256218615738 1107 58-59 2.7862502225268955 1107 60-61 2.6011242944989057 1107 62-63 0.34052140620780236 1107 64-65 2.1170364679020466 1107 66-67 3.998728815524718 1107 68-69 4.865661791785698 1107 70-71 4.0464187937060245 1107 72-73 2.16485769128246 1107 74-75 2.399987288322553 1107 76-77 2.986968706819173 1107 78-79 3.8295928325260356 1107 80-81 1.9074127370894765 1107 82-83 1.453728927821782 1107 84-85 2.2478223713726173 1107 86-87 2.2974864672287225 1107 88-89 2.570162833242634 1107 90-91 1.3624005754987536 1107 92-93 2.146198722271592 1107 94-95 2.2385338879122365 1107 96-97 2.234210748525758 1107 98-99 1.7909032920811434 1107 100 0.12102746629674144 1108 1 4.313087014728701 1108 2 -3.1119330947361483 1108 3 0.058364530385617286 1108 4 6.663654271437512 1108 5 7.045791134586114 1108 6 6.765829176756853 1108 7 4.341818577302568 1108 8 4.993039805308818 1108 9 4.934973017693245 1108 10-11 3.3974435474236415 1108 12-13 2.437412373186646 1108 14-15 4.0021289248997824 1108 16-17 4.6797214802947416 1108 18-19 4.018440406775671 1108 20-21 4.824226381832293 1108 22-23 4.159623209943728 1108 24-25 2.8284118398946063 1108 26-27 4.723551925164344 1108 28-29 6.160455835143395 1108 30-31 4.516071602350653 1108 32-33 4.602521674947699 1108 34-35 3.044665182013837 1108 36-37 1.2569790491417656 1108 38-39 2.596387313116548 1108 40-41 5.494308317121526 1108 42-43 3.2354924260241553 1108 44-45 3.717076020554906 1108 46-47 4.84414989793747 1108 48-49 4.982807395223059 1108 50-51 2.1476023066720593 1108 52-53 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2212 22-23 -18.61524944437601 2212 24-25 -25.118424388393237 2212 26-27 -17.165486970537387 2212 28-29 -17.987374307400664 2212 30-31 -6.081008876070637 2212 32-33 -17.17643706835473 2212 34-35 -13.698789447125497 2212 36-37 -17.6286419961103 2212 38-39 -23.626069262565835 2212 40-41 -10.235384028926312 2212 42-43 -13.964244039535243 2212 44-45 -17.073906594802008 2212 46-47 -17.003557000836967 2212 48-49 -17.629420959740305 2212 50-51 -9.008594215333893 2212 52-53 -2.254804925465706 2212 54-55 -23.447506446881107 2212 56-57 -23.372063994015576 2212 58-59 -12.425609600968333 2212 60-61 -6.2082228219668885 2212 62-63 -3.16240515998442 2212 64-65 -17.254131254995997 2212 66-67 -17.61990642742299 2212 68-69 -14.577735457417068 2212 70-71 -9.686361932182168 2212 72-73 -17.488469395434556 2212 74-75 -6.292410079294928 2212 76-77 -10.92576086978056 2212 78-79 -4.6684062359158744 2212 80-81 -2.3262612889875243 2212 82-83 -3.055990099977638 2212 84-85 -5.53163252857021 2212 86-87 -5.075205512290907 2212 88-89 -3.4367818448038356 2212 90-91 -4.169982099781521 2212 92-93 -5.387820918268282 2212 94-95 -3.6868598410204463 2212 96-97 -5.406823651574367 2212 98-99 -3.9598742969854435 2212 100 -3.8901445464855264 2213 1 -21.08951844432837 2213 2 -16.90486113443838 2213 3 -19.872404700383612 2213 4 -18.979521907222537 2213 5 -21.15731060238659 2213 6 -21.679083974607913 2213 7 -10.627050504583288 2213 8 -10.350219549529893 2213 9 -4.390104500917179 2213 10-11 -17.58528806547959 2213 12-13 -16.2352538302873 2213 14-15 -23.53806958626647 2213 16-17 -17.868038519705255 2213 18-19 -18.475721975358326 2213 20-21 -17.56975297300642 2213 22-23 -19.759438229262226 2213 24-25 -7.577144388393236 2213 26-27 -23.1453066356297 2213 28-29 -24.0530677380576 2213 30-31 -19.370790035366472 2213 32-33 -23.268321997508874 2213 34-35 -17.783911095901793 2213 36-37 -23.70356685656543 2213 38-39 -11.487349262565836 2213 40-41 -17.085624784615447 2213 42-43 -23.21971849208999 2213 44-45 -17.084084078700677 2213 46-47 -16.931111144246156 2213 48-49 -6.235622282196296 2213 50-51 -23.319457246677818 2213 52-53 -17.704025775615982 2213 54-55 -17.632466446881107 2213 56-57 -17.500223994015577 2213 58-59 -17.97957968254841 2213 60-61 -19.244467321752204 2213 62-63 -17.547542403436548 2213 64-65 -17.6019668582592 2213 66-67 -8.777468162073056 2213 68-69 -22.68464829554502 2213 70-71 -17.35167848005679 2213 72-73 -9.435969404021932 2213 74-75 -4.330438348938554 2213 76-77 -3.2343790921936133 2213 78-79 -4.834888949526867 2213 80-81 -3.448809472757379 2213 82-83 -3.345256841068231 2213 84-85 -3.025782326766862 2213 86-87 -3.2489820000539 2213 88-89 -2.788673506461201 2213 90-91 -2.772480133272289 2213 92-93 -3.6342534129011703 2213 94-95 -3.6304696306297224 2213 96-97 -3.3315179237942054 2213 98-99 -3.6958562978441805 2213 100 -3.3296750574344287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1062.0 3 1022.0 4 4966.0 5 16539.0 6 25319.0 7 32331.0 8 30660.0 9 31515.0 10 38706.0 11 48002.0 12 59254.0 13 77690.0 14 63166.0 15 67684.0 16 74894.0 17 78024.0 18 77139.0 19 77086.0 20 75571.0 21 72846.0 22 75400.0 23 82200.0 24 88906.0 25 97666.0 26 107268.0 27 115348.0 28 125560.0 29 132365.0 30 140959.0 31 153741.0 32 173735.0 33 209661.0 34 252108.0 35 291460.0 36 313280.0 37 261789.0 38 74708.0 39 124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.96743636255015 21.468757959975495 14.166638678265853 24.397166999208498 2 14.59407896033299 21.91381699998029 39.96785708811785 23.524246951568863 3 16.72480967459113 27.39072130559971 31.032433303153343 24.852035716655816 4 11.676932459270057 18.62343841986534 39.93005192026045 29.769577200604147 5 12.627237358941478 37.92492270445491 36.77539521344802 12.672444723155593 6 29.07438449784093 38.89225860946734 19.23649454970898 12.796862342982749 7 25.070512233212177 32.13641845306113 25.679613780079425 17.113455533647265 8 24.148712457483974 35.84266964205128 22.672994456537026 17.335623443927723 9 24.48906298092581 17.06468633288727 22.69824131337115 35.74800937281577 10-11 22.62392075941832 27.576045358986097 30.05991630775312 19.74011757384247 12-13 23.663171202485504 25.345400324495365 29.740183696603967 21.251244776415167 14-15 21.230069979241993 26.894766417763606 27.10868379581401 24.766479807180392 16-17 20.79182483854047 30.25446741637085 27.84516725234517 21.108540492743508 18-19 20.723464292126927 29.396522413240326 29.306613198184948 20.5734000964478 20-21 22.170884840496853 28.75642649934032 28.2700159332664 20.80267272689643 22-23 21.26672460929803 28.8612178974968 28.650105854120383 21.221951639084782 24-25 21.52369125408403 28.709126945137847 28.227081345791788 21.540100454986337 26-27 21.43676881725099 28.607465306263602 28.360921197141288 21.59484467934412 28-29 21.28728208401893 28.5672098859036 28.737685796110636 21.407822233966833 30-31 21.874401431135624 28.901816583529016 28.117812248977142 21.10596973635822 32-33 21.05204775271399 28.734568109995077 28.62745893785158 21.58592519943935 34-35 21.185772219791538 28.31373031311008 28.48679312225746 22.013704344840917 36-37 21.69428606147126 28.857312330765435 28.03483565619098 21.413565951572323 38-39 21.44812083517399 28.575396809186703 28.29715331461648 21.679329041022825 40-41 21.64935168372511 28.55937074825366 27.981006467501857 21.810271100519373 42-43 21.378697879501633 28.5939090626735 28.49776940148515 21.52962365633972 44-45 21.80265244605924 28.187363339694027 28.403309514304237 21.606674699942495 46-47 21.635330925442826 28.6189039147818 28.449880855228088 21.295884304547283 48-49 21.506136925303668 28.37188146440769 28.507480058295258 21.614501551993385 50-51 21.755805734930508 28.376764401683918 28.23267376967497 21.634756093710603 52-53 21.254178984946044 28.39167436872973 28.43279348590544 21.92135316041879 54-55 21.710649310183662 28.43021343627205 28.146035647723405 21.713101605820885 56-57 21.794993858887356 28.335196000664308 28.106418488991007 21.763391651457322 58-59 21.50468936615671 27.949243100363887 28.50781219297851 22.038255340500896 60-61 21.904986585054615 28.373299361673077 28.05913299347375 21.66258105979856 62-63 21.45978356447219 28.47603935402285 28.350853381923574 21.713323699581387 64-65 21.474948823438673 27.96069651591092 28.437606430642763 22.126748230007646 66-67 21.57381874563815 28.20207253448762 28.144195831695956 22.07991288817827 68-69 22.01618846941508 28.118705817555238 28.07683784172028 21.78826787130939 70-71 22.00839920705548 28.1695747530693 27.958814451516485 21.863211588358734 72-73 21.701107290946904 28.272889150524904 28.337254394406557 21.688749164121628 74-75 21.926513537993756 27.902738173114145 27.97203453881114 22.19871375008096 76-77 21.878660497185482 28.28961760216766 28.01516906570154 21.81655283494532 78-79 21.894636504289792 28.073308733155915 28.15866264101412 21.87339212154017 80-81 21.987511271592343 27.98819638290334 28.008360708866313 22.015931636638005 82-83 21.51226765593957 28.167866210688068 28.41540073698438 21.904465396387977 84-85 21.731847487487183 27.819119322259056 28.052146505528242 22.396886684725523 86-87 21.919953112105606 28.093667975235338 28.028718560282012 21.957660352377047 88-89 21.678939072856632 27.73602406580177 28.342467489257768 22.24256937208383 90-91 21.94308473221324 27.73545197164109 28.03434837001364 22.28711492613203 92-93 21.598755426122317 28.016305840980273 28.235892081982527 22.149046650914887 94-95 21.513126977629835 27.646788061181205 28.210729870178895 22.629355091010066 96-97 21.683718627425183 27.808538456695725 28.207844870849463 22.29989804502963 98-99 21.67711015732828 27.630712039662047 28.185640927653992 22.506536875355685 100 21.801700040749505 27.453590383336806 28.027822948075453 22.716886627838235 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 411.0 1 473.0 2 441.5 3 350.5 4 410.0 5 583.5 6 859.5 7 1200.0 8 1782.0 9 2680.0 10 3671.5 11 4819.5 12 6101.5 13 7215.5 14 8314.5 15 9444.0 16 10520.0 17 11678.5 18 12800.0 19 13852.0 20 15120.0 21 16457.0 22 17724.5 23 19403.5 24 21599.5 25 24372.0 26 28314.0 27 33893.0 28 41618.0 29 51529.0 30 63529.0 31 76835.0 32 90122.0 33 103163.0 34 115354.0 35 125922.0 36 134745.5 37 141909.5 38 147157.5 39 149867.5 40 150289.0 41 148797.0 42 144988.5 43 139329.5 44 133151.5 45 126282.5 46 119378.0 47 113328.5 48 107878.5 49 103412.5 50 99720.0 51 93291.5 52 83817.5 53 75275.0 54 68544.5 55 62536.5 56 56555.0 57 50619.5 58 44845.0 59 39310.5 60 33723.5 61 28419.0 62 23422.5 63 18879.5 64 15274.5 65 12202.0 66 9542.5 67 7404.0 68 5686.5 69 4387.5 70 3425.5 71 2605.0 72 1922.0 73 1469.5 74 1119.5 75 799.0 76 564.0 77 428.5 78 329.5 79 237.0 80 160.0 81 111.0 82 75.5 83 45.5 84 31.5 85 30.5 86 24.5 87 15.0 88 7.0 89 4.5 90 3.5 91 1.5 92 1.0 93 0.5 94 1.5 95 2.0 96 1.0 97 0.5 98 0.0 99 1.0 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.816006229460945 2 24.91926853152295 3 6.278916332443228 4 22.230621570659284 5 12.179560595042844 6 9.791372240430452 7 8.190058836842153 8 8.626197820455845 9 5.0176806436817385 10-11 6.840954212256498 12-13 9.712531310329409 14-15 7.261749696006908 16-17 7.9099851661235245 18-19 6.764209313833207 20-21 7.991922195304121 22-23 5.7893079917167025 24-25 5.325715102990503 26-27 10.07699423029607 28-29 6.476285798988096 30-31 4.374500308787935 32-33 8.856528412599863 34-35 7.098492117550936 36-37 6.8770388360421 38-39 6.097397249239264 40-41 8.150001342556239 42-43 6.579374938694499 44-45 6.489930554223655 46-47 6.09386276444933 48-49 4.071822374877868 50-51 6.7114797326066356 52-53 4.514194655311016 54-55 5.030859614100018 56-57 4.890124101514787 58-59 3.7863373805467435 60-61 4.184432704231573 62-63 3.1403075385354735 64-65 4.708358426348735 66-67 2.596969549180575 68-69 5.109563548666567 70-71 2.5380477697948955 72-73 1.5611052142144375 74-75 0.7965879344196897 76-77 1.1151025521172113 78-79 1.7240477029410748 80-81 2.0991003777240875 82-83 0.7737233797127149 84-85 1.2042318468587199 86-87 0.3794091327799079 88-89 0.6446872857732329 90-91 0.2290154350128803 92-93 0.27273071006977456 94-95 0.5635311311392494 96-97 0.5537496499764094 98-99 0.48406002157953654 100 0.3534484789933787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3649754.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.00400055153186 #Duplication Level Percentage of deduplicated Percentage of total 1 97.29708119766063 93.4090903696265 2 2.2165025271790966 4.255862196835474 3 0.29446502917009193 0.8480946246855704 4 0.08075921441315327 0.3101283066024659 5 0.039649849893910634 0.19032721055415758 6 0.020911438896180735 0.12045490747933559 7 0.012863053896069995 0.08644332433328814 8 0.009839620525554477 0.0755714347489757 9 0.005138963506576106 0.044402494978767096 >10 0.02148934026376808 0.31630998816782674 >50 4.064101453042795E-4 0.027264517333951733 >100 7.502700817125685E-4 0.17041443588922353 >500 8.585062493800256E-5 0.04945202017677586 >1k 5.7233743054645577E-5 0.09618416858772637 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.7399106898711528E-5 0.0 5 0.0 0.0 0.0 5.4798213797423056E-5 0.0 6 0.0 0.0 0.0 5.4798213797423056E-5 0.0 7 0.0 0.0 0.0 1.3699553449355765E-4 0.0 8 0.0 0.0 0.0 1.3699553449355765E-4 0.0 9 0.0 0.0 0.0 1.9179374829098072E-4 0.0 10-11 0.0 0.0 0.0 1.9179374829098072E-4 0.0 12-13 0.0 0.0 0.0 2.1919285518969225E-4 0.0 14-15 1.3699553449355764E-5 0.0 0.0 3.013901758858268E-4 0.0 16-17 2.7399106898711528E-5 0.0 0.0 3.150897293351826E-4 1.3699553449355764E-5 18-19 2.7399106898711528E-5 0.0 0.0 3.561883896832499E-4 2.7399106898711528E-5 20-21 4.109866034806729E-5 0.0 0.0 3.8358749658196144E-4 2.7399106898711528E-5 22-23 5.4798213797423056E-5 0.0 0.0 4.1098660348067294E-4 2.7399106898711528E-5 24-25 5.4798213797423056E-5 1.3699553449355764E-5 0.0 5.342825845248748E-4 2.7399106898711528E-5 26-27 6.849776724677882E-5 2.7399106898711528E-5 0.0 6.712781190184325E-4 2.7399106898711528E-5 28-29 8.219732069613459E-5 4.109866034806729E-5 0.0 9.452691880055478E-4 2.7399106898711528E-5 30-31 8.219732069613459E-5 8.219732069613459E-5 0.0 0.0024933187277827494 2.7399106898711528E-5 32-33 8.219732069613459E-5 8.219732069613459E-5 0.0 0.006726480743633681 2.7399106898711528E-5 34-35 8.219732069613459E-5 1.0959642759484611E-4 0.0 0.01271318560100215 2.7399106898711528E-5 36-37 8.219732069613459E-5 1.0959642759484611E-4 0.0 0.022727559172481215 2.7399106898711528E-5 38-39 1.0959642759484611E-4 1.3699553449355765E-4 0.0 0.03971500544968236 2.7399106898711528E-5 40-41 1.0959642759484611E-4 1.3699553449355765E-4 0.0 0.0604835284789057 2.7399106898711528E-5 42-43 1.0959642759484611E-4 1.3699553449355765E-4 0.0 0.08215622203578651 2.7399106898711528E-5 44-45 1.0959642759484611E-4 1.3699553449355765E-4 0.0 0.10756889368434147 2.7399106898711528E-5 46-47 1.0959642759484611E-4 1.5069508794291343E-4 0.0 0.13751611752463316 2.7399106898711528E-5 48-49 1.0959642759484611E-4 1.6439464139226918E-4 0.0 0.17161430606007966 2.7399106898711528E-5 50-51 1.0959642759484611E-4 1.7809419484162494E-4 0.0 0.20871269680093507 2.7399106898711528E-5 52-53 1.2329598104420187E-4 2.0549330174033647E-4 0.0 0.24789341966609257 2.7399106898711528E-5 54-55 1.3699553449355765E-4 2.1919285518969223E-4 0.0 0.29310194604896656 2.7399106898711528E-5 56-57 1.3699553449355765E-4 2.1919285518969223E-4 0.0 0.34672199824974503 2.7399106898711528E-5 58-59 1.3699553449355765E-4 2.1919285518969223E-4 0.0 0.40557527986817743 2.7399106898711528E-5 60-61 1.5069508794291343E-4 2.465919620884038E-4 0.0 0.4686617235024607 2.7399106898711528E-5 62-63 1.6439464139226918E-4 2.465919620884038E-4 0.0 0.5342962840783242 2.7399106898711528E-5 64-65 1.6439464139226918E-4 2.465919620884038E-4 0.0 0.6098356217980718 2.7399106898711528E-5 66-67 1.6439464139226918E-4 2.465919620884038E-4 0.0 0.7016226299087556 2.7399106898711528E-5 68-69 1.6439464139226918E-4 2.6029151553775957E-4 0.0 0.8006293026872495 2.7399106898711528E-5 70-71 1.6439464139226918E-4 2.739910689871153E-4 0.0 0.9165412244222487 2.7399106898711528E-5 72-73 1.6439464139226918E-4 2.739910689871153E-4 0.0 1.0399605014474949 2.7399106898711528E-5 74-75 1.6439464139226918E-4 2.739910689871153E-4 0.0 1.1716406092027025 2.7399106898711528E-5 76-77 1.9179374829098072E-4 2.739910689871153E-4 0.0 1.318020337809069 2.7399106898711528E-5 78-79 1.9179374829098072E-4 2.739910689871153E-4 0.0 1.4816889028685223 2.7399106898711528E-5 80-81 1.9179374829098072E-4 3.013901758858268E-4 0.0 1.6670164619314067 2.7399106898711528E-5 82-83 1.9179374829098072E-4 3.013901758858268E-4 0.0 1.874770189990887 2.7399106898711528E-5 84-85 1.9179374829098072E-4 3.2878928278453837E-4 0.0 2.091483426006246 2.7399106898711528E-5 86-87 1.9179374829098072E-4 3.2878928278453837E-4 0.0 2.326197875254058 2.7399106898711528E-5 88 1.9179374829098072E-4 3.2878928278453837E-4 2.7399106898711528E-5 2.522991960554054 2.7399106898711528E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9735 0.0 17.65368 1 GTATACG 365 8.432489E-7 16.696644 1 TACATGG 10280 0.0 16.11982 2 ACATGGG 10730 0.0 15.327587 3 ATAGCGC 235 0.005816275 14.043834 3 TAGCGCT 355 3.15148E-5 13.944685 4 GGTACCG 255 0.0012221284 13.666429 5 CTAGACT 1260 0.0 13.53274 4 TATACCG 335 4.665379E-5 13.375034 5 GTATTAA 1875 0.0 13.326149 1 CATGGGG 4425 0.0 13.30041 4 GTATAGA 1335 0.0 13.238501 1 GTATATG 2055 0.0 13.048571 1 GTCTAAG 1125 0.0 13.0011215 1 GAACCGT 345 6.1705665E-5 12.982307 6 GTAGACG 255 0.009982148 12.942357 3 TAAGGGT 1275 0.0 12.942126 4 GTATTAC 1180 0.0 12.911601 1 GTCTCGC 525 2.5818754E-6 12.768958 1 CTATACT 1465 0.0 12.765441 4 >>END_MODULE