FastQCFastQC Report
Wed 25 May 2016
SRR1295123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3649754
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT57450.15740786913309773No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40890.11203494810883144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102400.018.9390581
TCAACGC109800.017.4264684
CAACGCA108950.017.389825
AACGCAG109550.017.3170556
ATCAACG111100.017.1358093
TATCAAC115550.016.6406082
TATACCG2002.988275E-416.4545255
GTACATG131900.016.022781
GAGTACT39300.016.00963812-13
CGCAGAG120400.015.6774278
ACGCAGA120650.015.6060267
CTAACGC2155.186118E-415.3046453
TACATGG135500.015.2172792
TAGAGCG2851.7454919E-514.8461884
TCTATAC14050.014.7210913
AGTACTT47250.014.70717212-13
ACATGGG134000.014.6283363
CTAGGAC14450.014.3135873
GTATATA29150.014.1590221
CATGGGG48400.013.9873184
Produced by FastQC (version 0.11.5)