##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295123_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3649754 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27065714565968 33.0 33.0 33.0 27.0 33.0 2 29.96233526971955 33.0 33.0 33.0 27.0 33.0 3 30.79716660355739 33.0 33.0 33.0 27.0 33.0 4 35.080635845593974 37.0 37.0 37.0 33.0 37.0 5 35.38801272633717 37.0 37.0 37.0 33.0 37.0 6 35.11671745547782 37.0 37.0 37.0 33.0 37.0 7 35.01145364865687 37.0 37.0 37.0 33.0 37.0 8 34.98849730694178 37.0 37.0 37.0 33.0 37.0 9 34.96338273757629 37.0 37.0 37.0 33.0 37.0 10-11 34.93415679522511 37.0 37.0 37.0 33.0 37.0 12-13 34.85323736887472 37.0 37.0 37.0 30.0 37.0 14-15 36.27223218331975 37.0 37.0 40.0 27.0 40.0 16-17 36.26322458993127 37.0 37.0 40.0 27.0 40.0 18-19 36.28479056396678 38.5 37.0 40.0 27.0 40.0 20-21 36.28758116300441 40.0 37.0 40.0 27.0 40.0 22-23 36.24066128840464 37.0 37.0 40.0 27.0 40.0 24-25 36.20911779259643 37.0 37.0 40.0 27.0 40.0 26-27 36.18785129079932 37.0 37.0 40.0 27.0 40.0 28-29 36.134018073546876 37.0 37.0 40.0 27.0 40.0 30-31 36.04747758342069 37.0 37.0 40.0 27.0 40.0 32-33 36.00818438722172 37.0 37.0 40.0 27.0 40.0 34-35 35.91956772977028 37.0 37.0 40.0 27.0 40.0 36-37 35.91093331221775 37.0 37.0 40.0 27.0 40.0 38-39 35.85610084953671 37.0 37.0 40.0 27.0 40.0 40-41 35.80723248744984 37.0 37.0 40.0 27.0 40.0 42-43 35.722035649525964 37.0 37.0 40.0 27.0 40.0 44-45 35.63036303268659 37.0 37.0 40.0 27.0 40.0 46-47 35.54129223503831 37.0 37.0 40.0 27.0 40.0 48-49 35.44507862721707 37.0 37.0 40.0 27.0 40.0 50-51 35.34903831874696 37.0 33.0 40.0 27.0 40.0 52-53 35.253759705448644 37.0 33.0 40.0 27.0 40.0 54-55 35.14164461495213 37.0 33.0 40.0 27.0 40.0 56-57 35.023695295628144 37.0 33.0 40.0 27.0 40.0 58-59 34.89589942226243 37.0 33.0 40.0 27.0 40.0 60-61 34.73854251546817 37.0 33.0 40.0 27.0 40.0 62-63 34.56586156217652 37.0 33.0 40.0 27.0 40.0 64-65 34.38598957628378 37.0 33.0 40.0 27.0 40.0 66-67 34.16659355671642 37.0 33.0 40.0 24.5 40.0 68-69 33.952228972144425 37.0 33.0 37.0 22.0 40.0 70-71 33.72302585324928 37.0 33.0 37.0 22.0 40.0 72-73 33.504566198160205 37.0 33.0 37.0 22.0 40.0 74-75 33.26280428763144 37.0 33.0 37.0 22.0 40.0 76-77 33.026719609047625 37.0 33.0 37.0 22.0 38.5 78-79 32.78903906948249 37.0 33.0 37.0 22.0 37.0 80-81 32.567585376987054 37.0 33.0 37.0 22.0 37.0 82-83 32.355145579674684 37.0 33.0 37.0 22.0 37.0 84-85 32.18021474872005 33.0 33.0 37.0 22.0 37.0 86-87 32.20409087845373 33.0 33.0 37.0 22.0 37.0 88-89 32.205342332661324 33.0 33.0 37.0 22.0 37.0 90-91 32.21060994795813 33.0 33.0 37.0 22.0 37.0 92-93 32.17927221944274 33.0 33.0 37.0 22.0 37.0 94-95 32.206090602270734 33.0 33.0 37.0 22.0 37.0 96-97 32.1665040986324 33.0 33.0 37.0 22.0 37.0 98-99 32.090868590047435 33.0 33.0 37.0 22.0 37.0 100 31.560197481802884 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5692608654278928 1101 2 4.043406130873262 1101 3 2.3062523121737932 1101 4 1.1762682350682638 1101 5 0.5779442216962565 1101 6 0.5719379631010568 1101 7 0.556222573278589 1101 8 0.5073365440707178 1101 9 0.6061172400306916 1101 10-11 0.5946557291154235 1101 12-13 0.6662884073198398 1101 14-15 0.9389531064675509 1101 16-17 0.898051228306052 1101 18-19 0.9346053862539989 1101 20-21 0.933920211528239 1101 22-23 0.9339095500244596 1101 24-25 0.9283547568485844 1101 26-27 1.0009908977316755 1101 28-29 0.8301968801340891 1101 30-31 0.7991299234634752 1101 32-33 0.9865032001997847 1101 34-35 1.031692598859209 1101 36-37 0.9398852738988452 1101 38-39 0.9697470697217625 1101 40-41 0.9978708586983203 1101 42-43 1.0780557720964126 1101 44-45 1.0268240633489825 1101 46-47 1.0612289633429128 1101 48-49 1.0541511709060813 1101 50-51 1.0685165719016538 1101 52-53 0.9903670657872894 1101 54-55 1.0450902335228704 1101 56-57 1.077519413571963 1101 58-59 1.0567125999778924 1101 60-61 0.9833455968520397 1101 62-63 1.0876491586829502 1101 64-65 1.0503702262159464 1101 66-67 1.0484238516794875 1101 68-69 0.9165047509355304 1101 70-71 0.8917955611421604 1101 72-73 0.9154068530759076 1101 74-75 0.9717703981420698 1101 76-77 0.9540429930803356 1101 78-79 0.9020485107088518 1101 80-81 0.8995208207051562 1101 82-83 1.006948704723463 1101 84-85 0.9484500037348553 1101 86-87 0.9372489347106132 1101 88-89 0.8107373326951297 1101 90-91 0.8691008455351046 1101 92-93 0.9144974278917388 1101 94-95 0.894601854423744 1101 96-97 0.8738853171891883 1101 98-99 0.8753219057645794 1101 100 0.9256787995142517 1102 1 0.4285273411739645 1102 2 3.9902531483436974 1102 3 2.2180466742481393 1102 4 0.9941467262740034 1102 5 0.435551091537306 1102 6 0.47860065102477023 1102 7 0.44321936204513435 1102 8 0.48247096860201566 1102 9 0.5303192791242779 1102 10-11 0.5918423090663296 1102 12-13 0.5927902880202964 1102 14-15 0.7851334135197661 1102 16-17 0.8009545399960629 1102 18-19 0.8144146723897805 1102 20-21 0.7111433208333509 1102 22-23 0.7516773150790215 1102 24-25 0.8098978621274568 1102 26-27 0.8111024065090362 1102 28-29 0.6939401419716873 1102 30-31 0.7151626758738487 1102 32-33 0.797891625757039 1102 34-35 0.9141182251302666 1102 36-37 0.8292832529148129 1102 38-39 0.8290706401856482 1102 40-41 0.774458131814562 1102 42-43 0.8720126421635257 1102 44-45 0.8662197352847016 1102 46-47 0.8001074778554269 1102 48-49 0.7239570270962901 1102 50-51 0.5997415748850301 1102 52-53 0.7112173579872589 1102 54-55 0.706564584308893 1102 56-57 0.5804971379243256 1102 58-59 0.6467328010496445 1102 60-61 0.7045405886108682 1102 62-63 0.7644372474932624 1102 64-65 0.6657746276988092 1102 66-67 0.5847688830438287 1102 68-69 0.6202253566513463 1102 70-71 0.6370702063728189 1102 72-73 0.654887050464545 1102 74-75 0.6448902750214742 1102 76-77 0.6086717777785111 1102 78-79 0.551998017543994 1102 80-81 0.5599003092987616 1102 82-83 0.6504306153951163 1102 84-85 0.6226479591974368 1102 86-87 0.6258504313762252 1102 88-89 0.6166159162754248 1102 90-91 0.6252531496110585 1102 92-93 0.6589334559206819 1102 94-95 0.5865482597088025 1102 96-97 0.5470122195884564 1102 98-99 0.6352150213472925 1102 100 0.5986742950066635 1103 1 0.45143561170779734 1103 2 3.92716214475535 1103 3 2.225224056374852 1103 4 0.9750763387225803 1103 5 0.3841771118130026 1103 6 0.34874604333774784 1103 7 0.34706324020649504 1103 8 0.2828295725369756 1103 9 0.3383739403989736 1103 10-11 0.4090938284983139 1103 12-13 0.4223553516222296 1103 14-15 0.6228823575612239 1103 16-17 0.6119156870859399 1103 18-19 0.6187551127771869 1103 20-21 0.5323437262919128 1103 22-23 0.5187517692064461 1103 24-25 0.5124159525799428 1103 26-27 0.5262547832054949 1103 28-29 0.6054533297512918 1103 30-31 0.622065686329897 1103 32-33 0.6207716267162695 1103 34-35 0.7101973089193478 1103 36-37 0.5861516527415986 1103 38-39 0.47717367333115135 1103 40-41 0.5315506360423115 1103 42-43 0.6227053159164981 1103 44-45 0.6539561355671211 1103 46-47 0.6274709042200968 1103 48-49 0.560813888509422 1103 50-51 0.5661490422954643 1103 52-53 0.5423978225582857 1103 54-55 0.5534896570234906 1103 56-57 0.45934361015351044 1103 58-59 0.5161352292815877 1103 60-61 0.5166404507678664 1103 62-63 0.6579157379440943 1103 64-65 0.5316838957381904 1103 66-67 0.5235635477034961 1103 68-69 0.4957406307532324 1103 70-71 0.5359539962851443 1103 72-73 0.5590949512803078 1103 74-75 0.4965606241115168 1103 76-77 0.4281763546364701 1103 78-79 0.4823109797204097 1103 80-81 0.47812452345762324 1103 82-83 0.5641132970294294 1103 84-85 0.5869393129042351 1103 86-87 0.5079252844136342 1103 88-89 0.5300693053731429 1103 90-91 0.516825519030295 1103 92-93 0.5320674218837453 1103 94-95 0.45097452800044024 1103 96-97 0.4258186855273074 1103 98-99 0.47796761091872497 1103 100 0.5888213567464007 1104 1 0.365995060607883 1104 2 3.4835374886819004 1104 3 2.0418635990570415 1104 4 0.8769579258100748 1104 5 0.2328263002299451 1104 6 0.2585692915649389 1104 7 0.28065973192346405 1104 8 0.2700361551192856 1104 9 0.3113029034267569 1104 10-11 0.37917050019964194 1104 12-13 0.3218336329276781 1104 14-15 0.4712467905863491 1104 16-17 0.47309044357385943 1104 18-19 0.433895776645997 1104 20-21 0.48903944906249563 1104 22-23 0.33103238540392965 1104 24-25 0.33594322240228536 1104 26-27 0.3463633627447251 1104 28-29 0.3956385809918501 1104 30-31 0.35215923620923917 1104 32-33 0.5062586694402569 1104 34-35 0.5226761995855327 1104 36-37 0.4610243929514226 1104 38-39 0.45135791020899774 1104 40-41 0.3990419368603071 1104 42-43 0.502501245089455 1104 44-45 0.4700377323152125 1104 46-47 0.43551335139800074 1104 48-49 0.4208268188472388 1104 50-51 0.3112292902755627 1104 52-53 0.35296312540872066 1104 54-55 0.3836418207185943 1104 56-57 0.36310460140509804 1104 58-59 0.4316539104614421 1104 60-61 0.33783761241009813 1104 62-63 0.36432243236639295 1104 64-65 0.4096790059897586 1104 66-67 0.3696520276271045 1104 68-69 0.4587745413072497 1104 70-71 0.32579453140792225 1104 72-73 0.39246836680302977 1104 74-75 0.3381430392236666 1104 76-77 0.3669744277188869 1104 78-79 0.40872021933852665 1104 80-81 0.37504195693419007 1104 82-83 0.42608588880046483 1104 84-85 0.40953396353431515 1104 86-87 0.40135696076876215 1104 88-89 0.3925276208250885 1104 90-91 0.3748037325257805 1104 92-93 0.4103231360341084 1104 94-95 0.4962400922443386 1104 96-97 0.48906638323979124 1104 98-99 0.439739652407269 1104 100 0.3760252455083375 1105 1 0.4326367397683022 1105 2 3.6864556724471598 1105 3 2.0943516407504816 1105 4 0.9491825277947967 1105 5 0.32968501373034087 1105 6 0.3149625235203857 1105 7 0.2651978200222729 1105 8 0.2845199846330999 1105 9 0.3292528570213875 1105 10-11 0.281189133740007 1105 12-13 0.3479875202697116 1105 14-15 0.41533189089334144 1105 16-17 0.40508605291205413 1105 18-19 0.4791329794731496 1105 20-21 0.36079642771602494 1105 22-23 0.3858817464376898 1105 24-25 0.42663894596907426 1105 26-27 0.40096284871601995 1105 28-29 0.4868420863820049 1105 30-31 0.36489215546389175 1105 32-33 0.4206318399978315 1105 34-35 0.5692993165984532 1105 36-37 0.4656167396352231 1105 38-39 0.41112177827496055 1105 40-41 0.41966823085616767 1105 42-43 0.5511902934224295 1105 44-45 0.5423408664624247 1105 46-47 0.4542405949190922 1105 48-49 0.39980072475142236 1105 50-51 0.3755774733268993 1105 52-53 0.2884882200042753 1105 54-55 0.339431782880375 1105 56-57 0.3456062077447797 1105 58-59 0.38692270463577927 1105 60-61 0.3654479144019547 1105 62-63 0.3894223823913876 1105 64-65 0.24922262703637443 1105 66-67 0.36767533740078306 1105 68-69 0.3742221746334451 1105 70-71 0.35875929902411485 1105 72-73 0.36369257612693673 1105 74-75 0.33075923112771477 1105 76-77 0.22740760398789917 1105 78-79 0.35359778055791935 1105 80-81 0.3747073028326682 1105 82-83 0.38516973973214164 1105 84-85 0.38208340310024624 1105 86-87 0.3640523987640556 1105 88-89 0.335876482108727 1105 90-91 0.35473251813297324 1105 92-93 0.3444257275954712 1105 94-95 0.4237031464315315 1105 96-97 0.36786091454556313 1105 98-99 0.3223563797578777 1105 100 0.2644613845816579 1106 1 0.38451607137253774 1106 2 3.7780586621272363 1106 3 2.0960320450436676 1106 4 0.7989966598339393 1106 5 0.26447542634506505 1106 6 0.047234607488974234 1106 7 0.007680038046601112 1106 8 -0.026642570869135795 1106 9 0.09032824895374603 1106 10-11 0.022562521821377857 1106 12-13 0.058681685473530365 1106 14-15 0.15275315515992105 1106 16-17 0.17221589626059597 1106 18-19 0.1282020452133068 1106 20-21 0.12826589009598877 1106 22-23 0.012250700414114135 1106 24-25 0.09755683606401533 1106 26-27 0.160632538267933 1106 28-29 0.06392452125229653 1106 30-31 0.011116332876689228 1106 32-33 0.0700308061832331 1106 34-35 0.229497757980198 1106 36-37 0.09372319459868095 1106 38-39 0.05577131694582249 1106 40-41 0.14902435933587554 1106 42-43 0.11888761109263868 1106 44-45 0.14116294344577796 1106 46-47 0.06420282910057296 1106 48-49 -0.031114781252838952 1106 50-51 -0.009739109618202235 1106 52-53 -0.05480276661443639 1106 54-55 -0.02736360443565644 1106 56-57 0.04656455145472904 1106 58-59 0.010307293387739946 1106 60-61 -0.02065260918687528 1106 62-63 0.037751392978570664 1106 64-65 -0.04846017349571241 1106 66-67 -0.10442039978434536 1106 68-69 -0.15246946677502393 1106 70-71 -0.09797408674909036 1106 72-73 -0.13321501530370483 1106 74-75 -0.11817992444863279 1106 76-77 -0.046483793848501875 1106 78-79 -0.007001096945380425 1106 80-81 -0.11770756842643237 1106 82-83 -0.028983786596121774 1106 84-85 -0.00875045159474297 1106 86-87 0.057839185131303594 1106 88-89 0.10020782819365692 1106 90-91 0.09559788230662392 1106 92-93 0.08724369240204055 1106 94-95 0.021177376992838504 1106 96-97 -0.03500667579173111 1106 98-99 0.02825730788286407 1106 100 0.057817364245515535 1107 1 0.44146299048296456 1107 2 3.4936086143644722 1107 3 2.1522120288163435 1107 4 0.9590974977150495 1107 5 0.27865228294799493 1107 6 0.26088235158979245 1107 7 0.14034327769645927 1107 8 0.1257084012642835 1107 9 0.21774029984055687 1107 10-11 0.1854146854099028 1107 12-13 0.09245404193592321 1107 14-15 0.0881268947059155 1107 16-17 0.12118076842789094 1107 18-19 0.15631405744357352 1107 20-21 0.20929456008438052 1107 22-23 0.11798066389093265 1107 24-25 0.15936335324308004 1107 26-27 0.11522356148979895 1107 28-29 0.06520358174675067 1107 30-31 0.11082050961000078 1107 32-33 0.17795382268631244 1107 34-35 0.37975811616426824 1107 36-37 0.1650357194613079 1107 38-39 0.06753361396577162 1107 40-41 0.17029633149567047 1107 42-43 0.15270312649276008 1107 44-45 0.2798348888565414 1107 46-47 0.19449277195224823 1107 48-49 0.1892486293316935 1107 50-51 0.13533029192004165 1107 52-53 0.15257898796219393 1107 54-55 0.1245334224433634 1107 56-57 0.16212442923323778 1107 58-59 0.13305780445167414 1107 60-61 0.1729434347046208 1107 62-63 0.2490815700105884 1107 64-65 0.19416699867231557 1107 66-67 0.13458436338302704 1107 68-69 0.17323802846001257 1107 70-71 0.15438518007801605 1107 72-73 0.08956140204720953 1107 74-75 0.13620987246948602 1107 76-77 0.18066956091422526 1107 78-79 0.1503316073541754 1107 80-81 0.19505752818919575 1107 82-83 0.2783106513084661 1107 84-85 0.27362749897137917 1107 86-87 0.24857680578866947 1107 88-89 0.21477485532706098 1107 90-91 0.20150589688468656 1107 92-93 0.2156199955780025 1107 94-95 0.11367284846463477 1107 96-97 0.1114427583353752 1107 98-99 0.16587719273722001 1107 100 0.18978250749245973 1108 1 0.5398883868975908 1108 2 3.6111953098953613 1108 3 2.1964320964460633 1108 4 0.9779611462842084 1108 5 0.3144122442306525 1108 6 0.17654560117687623 1108 7 0.10144902710217707 1108 8 0.10286118181189607 1108 9 0.15070801476686313 1108 10-11 0.16055998535779992 1108 12-13 0.11559025957712521 1108 14-15 0.15173619848786046 1108 16-17 0.09001634786737611 1108 18-19 0.13449287263893694 1108 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2211 74-75 -0.0032677365056983376 2211 76-77 -0.13266402999953186 2211 78-79 -0.07540440796230286 2211 80-81 0.052488638786030606 2211 82-83 -0.4261184673220271 2211 84-85 -0.14874934563722064 2211 86-87 -0.07789942795269056 2211 88-89 0.027579145023647555 2211 90-91 -0.021433976707722735 2211 92-93 0.11547641108816009 2211 94-95 0.23427105709236784 2211 96-97 0.03233059888963652 2211 98-99 0.047928427807139684 2211 100 -0.2040437876383443 2212 1 0.6807401140150553 2212 2 4.163369642162909 2212 3 2.4727631664481002 2212 4 1.2099042692978443 2212 5 0.6334667378471224 2212 6 0.5253423562992054 2212 7 0.49812157850124805 2212 8 0.5684388000827667 2212 9 0.34180729485490957 2212 10-11 0.3752889609349168 2212 12-13 0.506857238557231 2212 14-15 0.6407956303785625 2212 16-17 0.6414005296214356 2212 18-19 0.6161515787578153 2212 20-21 0.6871586161856698 2212 22-23 0.5910636782589975 2212 24-25 0.6507015259469497 2212 26-27 0.5571486152451328 2212 28-29 0.550660227532255 2212 30-31 0.44653468159088305 2212 32-33 0.6809985590489092 2212 34-35 0.5986639141868011 2212 36-37 0.6308566891262188 2212 38-39 0.6094700037972345 2212 40-41 0.5028742253103289 2212 42-43 0.5072315709956285 2212 44-45 0.7028103273401598 2212 46-47 0.6314809536853048 2212 48-49 0.6709450508433008 2212 50-51 0.5646212181845129 2212 52-53 0.440179097714541 2212 54-55 0.4537927424668311 2212 56-57 0.537646650904172 2212 58-59 0.4673625750213901 2212 60-61 0.44340191377165183 2212 62-63 0.4026239932647826 2212 64-65 0.5556973450383538 2212 66-67 0.3874684736333833 2212 68-69 0.5700055295057211 2212 70-71 0.44398771583760777 2212 72-73 0.49149418128951083 2212 74-75 0.5934500411542203 2212 76-77 0.5723952252634419 2212 78-79 0.4490942197578107 2212 80-81 0.5383675504706176 2212 82-83 0.49850140388534214 2212 84-85 0.632281166575126 2212 86-87 0.398825245733196 2212 88-89 0.5715489678158256 2212 90-91 0.5867736116909619 2212 92-93 0.6525685064789783 2212 94-95 0.6311618434769208 2212 96-97 0.4657609202563826 2212 98-99 0.5450334256580476 2212 100 0.45782842936080925 2213 1 -0.6432611912661805 2213 2 -25.80356895809471 2213 3 -14.731656241022918 2213 4 -6.322878555949043 2213 5 -2.2976596168115293 2213 6 -1.3960975750017823 2213 7 -1.2776409977117211 2213 8 -0.9832875030516277 2213 9 -1.0870500001214722 2213 10-11 -1.0747086002501405 2213 12-13 -1.047780460025848 2213 14-15 -1.3265934293036992 2213 16-17 -1.435936902752971 2213 18-19 -1.3173193357977837 2213 20-21 -1.3110175624317577 2213 22-23 -1.3318999284391566 2213 24-25 -1.3639299210259992 2213 26-27 -1.461075377884967 2213 28-29 -1.4620525934209425 2213 30-31 -1.439315726309509 2213 32-33 -1.3884897363568456 2213 34-35 -1.2555566439926764 2213 36-37 -1.3363182357342396 2213 38-39 -1.382134083794 2213 40-41 -1.298551966188171 2213 42-43 -1.4038143371194423 2213 44-45 -1.3312435670351164 2213 46-47 -1.4528913262631775 2213 48-49 -1.4098039057904472 2213 50-51 -1.3870737582002022 2213 52-53 -1.549641632212463 2213 54-55 -1.4999891038191322 2213 56-57 -1.4359305410236942 2213 58-59 -1.4618187731967254 2213 60-61 -1.35174745505617 2213 62-63 -1.399965203815512 2213 64-65 -1.2939461414365283 2213 66-67 -1.3825218054563564 2213 68-69 -1.3844566431005418 2213 70-71 -1.1562248389069225 2213 72-73 -1.2302507736267572 2213 74-75 -1.490945596458495 2213 76-77 -1.3778687506919027 2213 78-79 -1.2487125642696704 2213 80-81 -1.436992106034321 2213 82-83 -1.5028804080511122 2213 84-85 -1.345580439264289 2213 86-87 -1.2462313450783107 2213 88-89 -1.4334051927681166 2213 90-91 -1.3283578266946137 2213 92-93 -1.28214373053261 2213 94-95 -1.4532578387901474 2213 96-97 -1.4648433219076331 2213 98-99 -1.5271363296017242 2213 100 -1.4838781228075035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 37.0 5 58.0 6 63.0 7 59.0 8 71.0 9 109.0 10 586.0 11 2626.0 12 7435.0 13 13627.0 14 17948.0 15 20121.0 16 21245.0 17 22077.0 18 22870.0 19 23561.0 20 25676.0 21 28356.0 22 30679.0 23 32793.0 24 34584.0 25 37167.0 26 41403.0 27 46912.0 28 55485.0 29 66059.0 30 79785.0 31 98220.0 32 123212.0 33 163731.0 34 222862.0 35 313551.0 36 473260.0 37 803282.0 38 815323.0 39 4904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.75818915962471 21.10215940903117 10.529331790865434 30.61031964047868 2 12.611505034506068 23.2960628995407 36.2423617911008 27.850070274852435 3 14.4856064271729 27.943390157254434 29.225503965472743 28.345499450099926 4 10.198178726379862 19.60814723194797 38.21035781554576 31.9833162261264 5 11.38529552202763 39.09466364439663 35.50221456287767 14.017826270698066 6 28.895112532753174 40.46173272312869 18.27395498251245 12.369199761605685 7 25.328415776236536 33.635728742054106 24.036652538191834 16.99920294351753 8 24.13786676074074 36.837839267647595 21.506424146336716 17.51786982527495 9 24.672298105728043 16.86082129614833 22.304752825290436 36.162127772833195 10-11 22.54387951006696 28.0217188224678 29.681138937528168 19.753262729937077 12-13 23.503286133003893 25.597231408617215 29.80648860851996 21.09299384985893 14-15 21.211909397355218 27.074670498769578 26.851789034237427 24.86163106963778 16-17 20.49628451961058 30.45549598233662 27.72387134651509 21.324348151537713 18-19 20.501201945147667 29.53376971164005 29.506581715140506 20.458446628071773 20-21 21.83690706952171 28.821635845869796 28.60585783782406 20.735599246784435 22-23 21.01202990683814 28.882862052016456 28.83764250494914 21.267465536196262 24-25 21.171607364273264 28.643471881508216 28.682838879905443 21.50208187431308 26-27 21.07494979447144 28.644985770361824 28.71954904700268 21.56051538816406 28-29 20.83861034185424 28.62080886315458 29.067962114881148 21.47261868011004 30-31 21.534113603991287 28.8980726634187 28.443736173239 21.12407755935102 32-33 20.67780774212728 28.78586845658179 29.100770404203622 21.4355533970873 34-35 20.8529757731141 28.38547981531243 29.160970872881936 21.60057353869153 36-37 21.35574838944701 28.804029372529953 28.512819416810142 21.327402821212896 38-39 21.07868268234586 28.837264868926134 28.76403579990393 21.320016648824076 40-41 21.317927007191265 28.612083886092233 28.63980003816965 21.430189068546852 42-43 21.00320128062185 28.61699087293878 28.82696616528889 21.55284168115048 44-45 21.359853533873444 28.29956723550666 28.71028727796792 21.630291952651973 46-47 21.351563804892045 28.75167317347142 28.574356863563448 21.322406158073086 48-49 21.191076613471793 28.650385679849894 28.631036940333942 21.527500766344374 50-51 21.457355737115254 28.519370364660944 28.328958772245343 21.694315125978452 52-53 21.07084426359467 28.674551391653004 28.451595300847522 21.803009043904808 54-55 21.406872736864823 28.602849537695224 28.262933057446375 21.727344667993574 56-57 21.45236254311424 28.47384673479994 28.270170366068026 21.8036203560178 58-59 21.05960137865739 28.16875742028567 28.771944006943983 21.99969719411296 60-61 21.572540370082034 28.543865547876457 28.117865924387118 21.765728157654387 62-63 21.03785715107838 28.708997266712817 28.41251206466496 21.840633517543846 64-65 21.04799659196121 28.13858626954446 28.622291549825917 22.19112558866841 66-67 21.218159428366363 28.42333632640679 28.30977035914552 22.048733886081333 68-69 21.47063430321639 28.402300049236658 28.300606420717273 21.826459226829687 70-71 21.49797045459871 28.278355430330638 28.164342954460647 22.059331160610004 72-73 21.200323336211422 28.509426368728523 28.466933336366267 21.823316958693788 74-75 21.518608857706642 28.08310951545714 28.189336220127164 22.208945406709056 76-77 21.350406420069625 28.46136686820732 28.239583541720503 21.94864317000255 78-79 21.340229096976675 28.426582615298653 28.360006324679098 21.873181963045567 80-81 21.45415598408876 28.246603954134063 28.18138496631083 22.11785509546635 82-83 21.01134465300526 28.45934644287455 28.43758961163371 22.09171929248648 84-85 21.170653737925544 28.10807826188702 28.246541769093948 22.474726231093484 86-87 21.328787402373116 28.376802599318935 28.11636377586286 22.17804622244509 88-89 21.07347020699567 28.095553535476704 28.443644937727303 22.387331319800328 90-91 21.270675919139055 28.060049733180797 28.243500777509094 22.42577357017105 92-93 20.905772118595316 28.422189308457945 28.342358740515582 22.329679832431157 94-95 20.876810734491915 27.9241021094103 28.489464730321153 22.709622425776637 96-97 21.072178190571552 28.161904896292462 28.260734652789584 22.505182260346405 98-99 21.000060141591664 28.068875411144496 28.303441318314647 22.62762312894919 100 21.08231803902713 27.980842734274265 28.1862558879024 22.750583338796208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 19.0 2 26.0 3 24.0 4 23.5 5 32.0 6 48.5 7 75.0 8 116.5 9 150.0 10 154.5 11 163.0 12 189.0 13 210.0 14 256.5 15 322.5 16 396.0 17 519.5 18 651.5 19 854.0 20 1145.5 21 1562.0 22 2254.5 23 3283.0 24 4921.5 25 7432.5 26 11557.5 27 17771.0 28 26535.5 29 38106.5 30 52339.0 31 68407.0 32 84995.5 33 100907.5 34 115733.0 35 129505.0 36 141372.5 37 150808.5 38 158099.0 39 162653.0 40 164237.5 41 163681.0 42 160292.5 43 154182.0 44 146411.5 45 139364.5 46 133016.5 47 126246.0 48 119692.5 49 114991.5 50 117449.0 51 110725.0 52 93482.5 53 83280.0 54 76407.5 55 69958.5 56 63800.5 57 57126.0 58 50180.5 59 44130.0 60 37750.0 61 31005.0 62 25451.5 63 20299.0 64 15691.5 65 12246.0 66 9289.0 67 6919.0 68 5171.5 69 3857.0 70 2854.5 71 2086.5 72 1545.0 73 1112.0 74 808.5 75 548.0 76 340.0 77 208.0 78 125.0 79 80.5 80 39.0 81 15.5 82 9.5 83 3.5 84 3.5 85 3.0 86 3.0 87 2.0 88 1.0 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.786820152810299 2 5.394418363539022 3 0.0 4 0.006027803517716537 5 0.15601051468126345 6 0.05523659950780244 7 0.03644081217528634 8 0.04287960229648354 9 0.04233162015850932 10-11 0.04334538711376164 12-13 0.04233162015850932 14-15 0.0445646473707543 16-17 0.03568733673557177 18-19 0.02972803098510201 20-21 0.02370022746738547 22-23 0.024371505586403906 24-25 0.020782222582672694 26-27 0.013822849430399967 28-29 0.018343702068687368 30-31 0.022097379713810848 32-33 0.0012740584707900862 34-35 0.0035892830037312106 36-37 0.004205762908952219 38-39 0.0067675794039817475 40-41 0.007055270026418218 42-43 0.007589552610943094 44-45 0.008480023585151219 46-47 0.017138141365144063 48-49 0.02568666271754206 50-51 0.022371370782797963 52-53 0.028864959117792598 54-55 0.02511128147266912 56-57 0.015959979768499465 58-59 0.01511060745463941 60-61 0.012466593638913746 62-63 0.0183026034083393 64-65 0.020124644017103618 66-67 0.021193209186153368 68-69 0.02875536269019775 70-71 0.02823477965912223 72-73 0.025220877900263967 74-75 0.0261387479813708 76-77 0.019097177508401935 78-79 0.015261302542582322 80-81 0.01397354451834288 82-83 0.008918409295530602 84-85 0.011425427576762709 86-87 0.00921979947141643 88-89 0.007712848591987296 90-91 0.007644350824740517 92-93 0.004096166481357373 94-95 0.0016713455208214032 96-97 0.0011918611500939514 98-99 9.178700811068363E-4 100 0.10770588921883502 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3649754.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.39754224159047 #Duplication Level Percentage of deduplicated Percentage of total 1 89.55813181290695 76.4804434457065 2 8.082941110910905 13.805266099106046 3 1.4501258714454812 3.71511556087166 4 0.425679488283961 1.4540792832843272 5 0.1750833994149956 0.7475845998671672 6 0.08855386481132822 0.45373694465288966 7 0.052492921061327295 0.313793651060342 8 0.03516052330075785 0.24020978190503156 9 0.02411839428360082 0.18536864351698218 >10 0.10219997825002794 1.4580262955905687 >50 0.0036281686020949587 0.20032021349159226 >100 0.0014021764818388357 0.2995113661586226 >500 1.929161117513376E-4 0.12430056455682392 >1k 2.57221453323232E-4 0.36449963876565705 >5k 3.215268166540394E-5 0.15774391146581523 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5745 0.15740786913309773 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4089 0.11203494810883144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.4798213797423056E-5 0.0 6 0.0 0.0 0.0 5.4798213797423056E-5 0.0 7 0.0 0.0 0.0 8.219732069613459E-5 0.0 8 0.0 0.0 0.0 8.219732069613459E-5 0.0 9 0.0 0.0 0.0 1.0959642759484611E-4 0.0 10-11 0.0 0.0 0.0 1.0959642759484611E-4 0.0 12-13 0.0 0.0 0.0 1.5069508794291343E-4 0.0 14-15 0.0 0.0 0.0 1.7809419484162494E-4 0.0 16-17 0.0 0.0 0.0 2.6029151553775957E-4 0.0 18-19 0.0 0.0 0.0 3.013901758858268E-4 0.0 20-21 0.0 0.0 0.0 3.8358749658196144E-4 0.0 22-23 0.0 0.0 0.0 4.3838571037938445E-4 0.0 24-25 0.0 1.3699553449355764E-5 0.0 5.479821379742306E-4 0.0 26-27 0.0 4.109866034806729E-5 0.0 6.43879012119721E-4 0.0 28-29 0.0 6.849776724677882E-5 0.0 0.0010822647224991053 0.0 30-31 0.0 1.3699553449355765E-4 0.0 0.003383789701990874 0.0 32-33 1.3699553449355764E-5 1.3699553449355765E-4 0.0 0.009973274911130998 0.0 34-35 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.019220473489446138 0.0 36-37 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.03398859210785165 0.0 38-39 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.05956565839779887 0.0 40-41 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.09071844294163386 0.0 42-43 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.12251510649758861 0.0 44-45 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.15998338518157662 0.0 46-47 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.20549330174033648 0.0 48-49 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.25526377942184597 0.0 50-51 2.7399106898711528E-5 1.3699553449355765E-4 0.0 0.3096647061692377 0.0 52-53 4.109866034806729E-5 1.3699553449355765E-4 0.0 0.3667507453927032 0.0 54-55 5.4798213797423056E-5 1.3699553449355765E-4 0.0 0.4328510907858447 0.0 56-57 5.4798213797423056E-5 1.3699553449355765E-4 0.0 0.5104590610764451 0.0 58-59 5.4798213797423056E-5 1.3699553449355765E-4 0.0 0.5974649250333036 0.0 60-61 5.4798213797423056E-5 1.3699553449355765E-4 0.0 0.6903889960802838 0.0 62-63 5.4798213797423056E-5 1.3699553449355765E-4 0.0 0.7856967894274518 0.0 64-65 8.219732069613459E-5 1.3699553449355765E-4 0.0 0.893525974627331 0.0 66-67 8.219732069613459E-5 1.6439464139226918E-4 0.0 1.0184933011923545 0.0 68-69 8.219732069613459E-5 1.6439464139226918E-4 0.0 1.1545435664979065 0.0 70-71 8.219732069613459E-5 1.7809419484162494E-4 0.0 1.310745874927461 0.0 72-73 8.219732069613459E-5 1.9179374829098072E-4 0.0 1.4740856507041298 0.0 74-75 8.219732069613459E-5 1.9179374829098072E-4 0.0 1.6476589929074672 0.0 76-77 8.219732069613459E-5 2.1919285518969223E-4 0.0 1.8389047590604737 0.0 78-79 9.589687414549036E-5 2.1919285518969223E-4 0.0 2.054946716956814 0.0 80-81 1.0959642759484611E-4 2.1919285518969223E-4 0.0 2.291332511725448 0.0 82-83 1.0959642759484611E-4 2.1919285518969223E-4 0.0 2.5512541393200747 0.0 84-85 1.0959642759484611E-4 2.1919285518969223E-4 0.0 2.824204042245039 2.7399106898711528E-5 86-87 1.5069508794291343E-4 2.1919285518969223E-4 0.0 3.1206349797821993 2.7399106898711528E-5 88 1.6439464139226918E-4 2.1919285518969223E-4 0.0 3.365240506620446 2.7399106898711528E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10240 0.0 18.939058 1 TCAACGC 10980 0.0 17.426468 4 CAACGCA 10895 0.0 17.38982 5 AACGCAG 10955 0.0 17.317055 6 ATCAACG 11110 0.0 17.135809 3 TATCAAC 11555 0.0 16.640608 2 TATACCG 200 2.988275E-4 16.454525 5 GTACATG 13190 0.0 16.02278 1 GAGTACT 3930 0.0 16.009638 12-13 CGCAGAG 12040 0.0 15.677427 8 ACGCAGA 12065 0.0 15.606026 7 CTAACGC 215 5.186118E-4 15.304645 3 TACATGG 13550 0.0 15.217279 2 TAGAGCG 285 1.7454919E-5 14.846188 4 TCTATAC 1405 0.0 14.721091 3 AGTACTT 4725 0.0 14.707172 12-13 ACATGGG 13400 0.0 14.628336 3 CTAGGAC 1445 0.0 14.313587 3 GTATATA 2915 0.0 14.159022 1 CATGGGG 4840 0.0 13.987318 4 >>END_MODULE