FastQCFastQC Report
Wed 25 May 2016
SRR1295122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3245993
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA650.0077582318.19020550-51
GTATATA17350.018.0416071
GTACATG119150.017.8232381
TACATGG119750.017.3207442
GTGTATA15050.017.1284221
GTCTATA9750.016.9966641
CATGGGG51150.016.5867274
ACATGGG118200.016.3724583
GTATAGG8000.016.1114221
GTATTAG11350.015.6821881
GTATCAA84050.015.4811311
TAGACCG2305.228363E-415.2880595
TAGTACG3001.1023022E-414.7871214
ACACCGT3856.932005E-714.3517886
GTATTAC11350.014.0598931
GTGTAGA14150.013.8802751
CTACACT12800.013.8629264
TATCAAC92550.013.6589152
GTGTAAG11800.013.5237111
GTATTAT14550.013.4986871
Produced by FastQC (version 0.11.5)