##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295122_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3245993 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.915515529454314 27.0 27.0 33.0 6.0 33.0 2 21.89289810544878 27.0 2.0 33.0 2.0 33.0 3 24.244426898024734 27.0 15.0 33.0 6.0 33.0 4 24.525621897521034 33.0 6.0 37.0 2.0 37.0 5 26.347216706875216 33.0 15.0 37.0 2.0 37.0 6 27.13767959450313 33.0 15.0 37.0 2.0 37.0 7 26.879524693984244 33.0 15.0 37.0 6.0 37.0 8 27.484919406788617 33.0 15.0 37.0 2.0 37.0 9 28.543588664547336 33.0 22.0 37.0 15.0 37.0 10-11 28.109093118808325 33.0 18.5 37.0 8.5 37.0 12-13 26.93580993551126 33.0 15.0 37.0 4.0 37.0 14-15 28.586322582950736 33.0 18.5 37.0 4.0 40.0 16-17 28.99796687793227 33.0 22.0 37.0 6.0 40.0 18-19 29.17662699827141 33.0 22.0 38.5 6.0 40.0 20-21 28.657145286511707 33.0 18.5 38.5 6.0 40.0 22-23 29.428931762945886 33.0 24.5 37.0 6.0 40.0 24-25 29.557276001519412 33.0 24.5 37.0 6.0 40.0 26-27 28.056102246677675 33.0 15.0 37.0 4.0 40.0 28-29 28.791778663724784 33.0 22.0 37.0 6.0 40.0 30-31 29.549116556936504 33.0 22.0 37.0 6.0 40.0 32-33 28.22305778231808 33.0 22.0 37.0 4.0 40.0 34-35 27.959490362425303 33.0 15.0 37.0 6.0 40.0 36-37 28.21418792338739 33.0 22.0 37.0 6.0 40.0 38-39 28.33961086792239 33.0 18.5 37.0 6.0 40.0 40-41 27.508994166037944 33.0 15.0 37.0 4.0 40.0 42-43 28.13612968358219 33.0 22.0 37.0 6.0 40.0 44-45 27.967779505377862 33.0 22.0 37.0 6.0 40.0 46-47 27.638017241565215 33.0 15.0 37.0 6.0 40.0 48-49 27.58518240797192 33.0 18.5 37.0 6.0 40.0 50-51 27.433380016531153 33.0 18.5 37.0 6.0 40.0 52-53 27.71174583555787 33.0 18.5 37.0 6.0 40.0 54-55 27.636826850828083 33.0 22.0 37.0 6.0 40.0 56-57 27.616035986522462 33.0 22.0 37.0 6.0 40.0 58-59 27.50743039187084 33.0 22.0 37.0 6.0 40.0 60-61 27.50534566772017 33.0 22.0 37.0 6.0 40.0 62-63 27.494406796317797 33.0 22.0 37.0 6.0 40.0 64-65 27.024778241974026 33.0 22.0 37.0 6.0 40.0 66-67 27.229424247063996 33.0 22.0 37.0 6.0 38.5 68-69 26.788830105302136 33.0 18.5 37.0 6.0 37.0 70-71 26.975573884478493 33.0 22.0 37.0 6.0 37.0 72-73 27.026589398067095 27.0 22.0 37.0 6.0 37.0 74-75 27.147794218903122 27.0 22.0 37.0 6.0 37.0 76-77 26.91835564648476 27.0 22.0 35.0 6.0 37.0 78-79 26.45892797673932 27.0 22.0 33.0 6.0 37.0 80-81 26.31491796193029 27.0 22.0 33.0 6.0 37.0 82-83 26.422612125164783 27.0 22.0 33.0 6.0 37.0 84-85 26.349704851489207 27.0 22.0 33.0 6.0 37.0 86-87 26.2072404345912 27.0 22.0 33.0 10.5 37.0 88-89 26.156478156299166 27.0 22.0 33.0 6.0 37.0 90-91 26.326346976102535 27.0 22.0 33.0 6.0 37.0 92-93 26.23773772771537 27.0 22.0 33.0 6.0 37.0 94-95 26.106793206270005 27.0 22.0 33.0 10.5 37.0 96-97 26.18153150669148 27.0 22.0 33.0 6.0 37.0 98-99 26.26241399781207 27.0 22.0 33.0 10.5 37.0 100 25.896667059972096 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.172219080571679 1101 2 11.08506329756922 1101 3 8.226617806469584 1101 4 12.533648138748987 1101 5 10.518472711672132 1101 6 9.718008096371143 1101 7 9.32853941950032 1101 8 8.921368785278215 1101 9 7.2767768337116046 1101 10-11 7.618958974045071 1101 12-13 8.947091613056937 1101 14-15 7.962231875721091 1101 16-17 8.098748067960795 1101 18-19 8.013232868721659 1101 20-21 7.0068533231638845 1101 22-23 7.257372673018594 1101 24-25 7.248618991507779 1101 26-27 8.402089440220696 1101 28-29 8.054301632770304 1101 30-31 5.373732985198661 1101 32-33 6.857282404037289 1101 34-35 8.53486993221168 1101 36-37 8.011092578359655 1101 38-39 8.011796094166566 1101 40-41 8.820355827147505 1101 42-43 8.453863526429163 1101 44-45 8.380951379563424 1101 46-47 7.188289772067577 1101 48-49 5.336924435083013 1101 50-51 6.640055601600345 1101 52-53 5.817531816076006 1101 54-55 3.9913393210746086 1101 56-57 6.057686960293125 1101 58-59 6.132354275976759 1101 60-61 6.650623921711123 1101 62-63 6.069864672723263 1101 64-65 6.58825683611245 1101 66-67 5.687757502447511 1101 68-69 6.736261096581774 1101 70-71 6.2477971367810845 1101 72-73 5.646380081669946 1101 74-75 4.919858936090549 1101 76-77 4.969930670317698 1101 78-79 5.017755465609163 1101 80-81 4.8286749928437835 1101 82-83 4.485010816980154 1101 84-85 4.200845114018616 1101 86-87 4.052384308235087 1101 88-89 3.9797670603786806 1101 90-91 3.738801988538352 1101 92-93 3.8137387594687944 1101 94-95 3.6840885366369562 1101 96-97 3.8441798860938334 1101 98-99 3.612127600344973 1101 100 3.8206660925681355 1102 1 6.645562097542406 1102 2 10.472258910089739 1102 3 8.051193721009962 1102 4 12.271966961852073 1102 5 10.359726894973036 1102 6 9.382253488269171 1102 7 6.309381835050171 1102 8 7.6435842066230215 1102 9 6.246789105742639 1102 10-11 7.239075390855618 1102 12-13 8.948446956871745 1102 14-15 6.228950218905549 1102 16-17 7.252529223846036 1102 18-19 7.336480328289426 1102 20-21 6.903972715104533 1102 22-23 6.419914049252263 1102 24-25 6.786580707643214 1102 26-27 8.302393804861413 1102 28-29 7.510987727615138 1102 30-31 4.210136122940792 1102 32-33 4.174381728314284 1102 34-35 4.793854733773248 1102 36-37 5.07368444349521 1102 38-39 4.48953000120974 1102 40-41 6.22180882953187 1102 42-43 6.859032944406021 1102 44-45 6.265219914745781 1102 46-47 5.384901564789015 1102 48-49 3.718617000911955 1102 50-51 4.926925167876927 1102 52-53 5.790265981958541 1102 54-55 5.296207023707769 1102 56-57 6.185897588541941 1102 58-59 5.917687493593668 1102 60-61 5.978156421277184 1102 62-63 4.125169996592213 1102 64-65 5.8838618959652464 1102 66-67 3.7666692504049664 1102 68-69 6.0540601702413674 1102 70-71 5.3526250999589 1102 72-73 5.015729146346295 1102 74-75 4.222930019470322 1102 76-77 3.3420609791891884 1102 78-79 4.260265507053909 1102 80-81 4.489633307525459 1102 82-83 3.867351425222207 1102 84-85 3.076330011503 1102 86-87 3.1640497720600003 1102 88-89 3.357492380045297 1102 90-91 3.3559498832607737 1102 92-93 3.300795238218086 1102 94-95 3.1208820650438156 1102 96-97 3.316652289249845 1102 98-99 3.1279501461671515 1102 100 3.1972253561244663 1103 1 6.647103177863315 1103 2 10.897104803590452 1103 3 7.93866788024749 1103 4 11.734189811365482 1103 5 10.210995916013133 1103 6 9.077058027353104 1103 7 8.050261538592135 1103 8 8.106175296773284 1103 9 7.010470012825319 1103 10-11 7.343563191285298 1103 12-13 8.661260778652636 1103 14-15 8.54609926588721 1103 16-17 8.067296785177216 1103 18-19 7.154277659049612 1103 20-21 8.318378859838635 1103 22-23 6.724710480624857 1103 24-25 5.060176419276832 1103 26-27 5.9176803774380815 1103 28-29 6.905169832113437 1103 30-31 6.110193073732152 1103 32-33 7.970844686868457 1103 34-35 7.048486001515318 1103 36-37 4.178657907504306 1103 38-39 6.305925276897778 1103 40-41 6.645347497384069 1103 42-43 7.430805789268899 1103 44-45 5.625316175603217 1103 46-47 6.379691076270539 1103 48-49 5.553466869277884 1103 50-51 6.449713843306284 1103 52-53 6.122529674136835 1103 54-55 4.976807453779639 1103 56-57 5.85795160877711 1103 58-59 5.451645532105083 1103 60-61 4.955072967358227 1103 62-63 2.7172043842754583 1103 64-65 4.9560968094572075 1103 66-67 4.106946897468692 1103 68-69 5.19724543662652 1103 70-71 3.8264229638075946 1103 72-73 4.420489748955397 1103 74-75 3.1892201622317238 1103 76-77 3.678246154157957 1103 78-79 3.025491673900845 1103 80-81 4.159468462309249 1103 82-83 3.382361952207912 1103 84-85 3.2403001094967436 1103 86-87 2.6807583384126303 1103 88-89 2.982172696058626 1103 90-91 2.5772988232282508 1103 92-93 2.342925774557564 1103 94-95 2.907535154753006 1103 96-97 2.924642544954324 1103 98-99 2.6452776788827386 1103 100 2.8163593668791442 1104 1 6.329632514030166 1104 2 9.639397882467012 1104 3 7.406167243265578 1104 4 12.021671439479839 1104 5 9.307561232817392 1104 6 8.133877933667765 1104 7 3.9896939490820493 1104 8 6.418242964421896 1104 9 6.514727976343597 1104 10-11 6.998368575553123 1104 12-13 7.232341385148597 1104 14-15 7.123789841697079 1104 16-17 7.05718283982068 1104 18-19 6.677742672643227 1104 20-21 7.425869995238546 1104 22-23 6.310882461488752 1104 24-25 5.871257011254681 1104 26-27 8.06769073195883 1104 28-29 6.97815691483585 1104 30-31 6.04102682472028 1104 32-33 7.413131999982834 1104 34-35 7.826092911553143 1104 36-37 6.452767820560556 1104 38-39 7.0937961250693995 1104 40-41 7.085977205722575 1104 42-43 6.923749958543379 1104 44-45 6.507677070627871 1104 46-47 6.519250540929892 1104 48-49 5.358964948687984 1104 50-51 5.5651596668671175 1104 52-53 5.913587070329008 1104 54-55 4.7497657491714556 1104 56-57 5.805703153373983 1104 58-59 3.8588872879207017 1104 60-61 4.064355128930025 1104 62-63 3.6223494144528985 1104 64-65 2.5676778716661524 1104 66-67 3.0413391399882634 1104 68-69 4.2034147917872 1104 70-71 2.5887302476140697 1104 72-73 2.4414197145443737 1104 74-75 3.099138745729139 1104 76-77 3.6158074607501796 1104 78-79 4.217195042858499 1104 80-81 3.1461758813000316 1104 82-83 2.967631840172725 1104 84-85 3.055852298925675 1104 86-87 0.7868890131903576 1104 88-89 0.3077656388486858 1104 90-91 2.2316863038089885 1104 92-93 2.446954507548959 1104 94-95 2.487793630967836 1104 96-97 2.8033980445944273 1104 98-99 2.442164374707989 1104 100 0.8827270020383082 1105 1 5.688199569544356 1105 2 -2.8652931078531694 1105 3 0.4095510440432726 1105 4 7.9798294237237 1105 5 8.330549199687216 1105 6 7.8045819958833675 1105 7 5.177699427879286 1105 8 5.60428765890541 1105 9 4.57567499931594 1105 10-11 4.878689949297183 1105 12-13 4.939755227384978 1105 14-15 5.702261202111853 1105 16-17 5.922277110065107 1105 18-19 5.939168171112655 1105 20-21 6.827250725683559 1105 22-23 1.971186001563943 1105 24-25 3.832566682802625 1105 26-27 6.6902346956296235 1105 28-29 5.776012737896437 1105 30-31 5.096648567088113 1105 32-33 6.870118385723256 1105 34-35 5.966511910845316 1105 36-37 6.0594209740863825 1105 38-39 3.98661669445983 1105 40-41 5.142594508058419 1105 42-43 5.385583609021854 1105 44-45 6.0473556049576835 1105 46-47 5.200204366435713 1105 48-49 4.8541153857967245 1105 50-51 5.843349733329706 1105 52-53 5.472967672077445 1105 54-55 5.144586171480089 1105 56-57 4.862668855735567 1105 58-59 1.8173086405217518 1105 60-61 4.2270763261759186 1105 62-63 -0.9624832340307776 1105 64-65 -0.5569132868788387 1105 66-67 0.661294923520618 1105 68-69 2.6780215777739826 1105 70-71 2.843375734500807 1105 72-73 2.5051782981465927 1105 74-75 2.5394681170463507 1105 76-77 1.6215615767425682 1105 78-79 3.0469005115438677 1105 80-81 1.458613946071182 1105 82-83 0.9176424116774662 1105 84-85 1.3409947384612657 1105 86-87 -0.3771356965528945 1105 88-89 1.398356999631158 1105 90-91 1.2426949172871957 1105 92-93 1.6207357969048317 1105 94-95 1.0626329900588694 1105 96-97 1.9491809334649304 1105 98-99 1.9229738761925965 1105 100 2.0233491580737173 1106 1 6.07115894526876 1106 2 10.17393514741569 1106 3 6.646022570537394 1106 4 11.492637667005738 1106 5 9.773476194177839 1106 6 9.162924667647438 1106 7 6.335284215425485 1106 8 7.199390237513377 1106 9 6.728546491699266 1106 10-11 5.525025741250161 1106 12-13 7.672677249386439 1106 14-15 7.599131476595655 1106 16-17 7.52667055646852 1106 18-19 7.010520087622879 1106 20-21 8.034904815796114 1106 22-23 5.9942236399438755 1106 24-25 4.836414716807727 1106 26-27 7.4033155850566 1106 28-29 6.027807698386766 1106 30-31 5.270050674510703 1106 32-33 7.124745322922163 1106 34-35 6.489824417376209 1106 36-37 5.660901010263043 1106 38-39 6.863163901278153 1106 40-41 6.582817141051482 1106 42-43 5.985472989154243 1106 44-45 4.225407169910522 1106 46-47 5.718252039875296 1106 48-49 5.658431542422925 1106 50-51 6.434922024794439 1106 52-53 5.502009531067738 1106 54-55 5.1069194459602905 1106 56-57 4.667896395158156 1106 58-59 4.851069019391794 1106 60-61 4.719065333852047 1106 62-63 4.733385516276996 1106 64-65 4.992592707132552 1106 66-67 2.8577204364737128 1106 68-69 3.278964368925834 1106 70-71 2.7943349204538848 1106 72-73 2.7616060811490897 1106 74-75 2.8007060026094344 1106 76-77 3.416253144289751 1106 78-79 3.99556808863246 1106 80-81 3.616157716587203 1106 82-83 2.131594327467038 1106 84-85 2.713984093250769 1106 86-87 3.154655144218154 1106 88-89 3.2992561313672866 1106 90-91 3.1381086367931665 1106 92-93 2.905683027264402 1106 94-95 1.4364539756446568 1106 96-97 1.5439773556749046 1106 98-99 1.9605268590254958 1106 100 2.9041800922410843 1107 1 6.062147533263431 1107 2 10.326744268486518 1107 3 6.286033546877398 1107 4 11.196872509648568 1107 5 9.17936754807215 1107 6 7.911475144484495 1107 7 3.777573765334889 1107 8 6.121012351492041 1107 9 5.5923397541758035 1107 10-11 4.500955368019042 1107 12-13 6.1047198692932305 1107 14-15 6.381852044453929 1107 16-17 7.041721580344781 1107 18-19 6.16137639064182 1107 20-21 5.448400579301161 1107 22-23 5.644215367949833 1107 24-25 4.49165675577164 1107 26-27 7.1278618436995735 1107 28-29 6.372144739372139 1107 30-31 5.125299730551735 1107 32-33 4.947217162063559 1107 34-35 6.577770386091547 1107 36-37 6.407680506510108 1107 38-39 6.383694833620762 1107 40-41 7.661205275561006 1107 42-43 6.139481214716351 1107 44-45 4.274264285697317 1107 46-47 3.460719045495022 1107 48-49 3.971670267569557 1107 50-51 4.826160885053795 1107 52-53 3.9271289520992667 1107 54-55 5.274756291283207 1107 56-57 5.188159130839399 1107 58-59 3.025640449576688 1107 60-61 2.7906097998462016 1107 62-63 0.45720470742866226 1107 64-65 1.9061642467043711 1107 66-67 4.133426102402694 1107 68-69 5.097950613572305 1107 70-71 4.29605665601235 1107 72-73 2.5211938137505534 1107 74-75 2.541305880087716 1107 76-77 3.231512944511163 1107 78-79 3.945727023860549 1107 80-81 2.095073083525957 1107 82-83 1.6826838358805212 1107 84-85 2.285602306529338 1107 86-87 2.32131615330103 1107 88-89 2.6503579379666036 1107 90-91 1.5661500482149613 1107 92-93 2.3013985944878925 1107 94-95 2.289953070954134 1107 96-97 2.438689867803152 1107 98-99 1.8835917928192174 1107 100 0.3917443047713043 1108 1 4.339876738229336 1108 2 -3.0810589949360487 1108 3 0.14725802115235354 1108 4 6.566107406527404 1108 5 6.993671101551374 1108 6 6.7057673449814565 1108 7 4.273542294715959 1108 8 5.141667807704895 1108 9 4.82854604973614 1108 10-11 3.289215826642348 1108 12-13 2.2237812814487334 1108 14-15 3.998983840451782 1108 16-17 4.747898920941431 1108 18-19 3.8768000529271447 1108 20-21 4.648777800833042 1108 22-23 4.126483272980256 1108 24-25 2.958822173413086 1108 26-27 4.722225533761048 1108 28-29 6.015954881763982 1108 30-31 4.3837761134332816 1108 32-33 4.485021526138109 1108 34-35 2.8181802638198867 1108 36-37 1.1265928658812179 1108 38-39 2.338862415807359 1108 40-41 5.321913235619807 1108 42-43 3.1246590027565553 1108 44-45 3.719374802986845 1108 46-47 4.737350534958129 1108 48-49 4.989511170754856 1108 50-51 2.2976998415850147 1108 52-53 0.5942425693825015 1108 54-55 2.5080826601583723 1108 56-57 2.804668942275107 1108 58-59 3.2893400915795965 1108 60-61 4.519602261753292 1108 62-63 3.8397237871568493 1108 64-65 4.231222141843716 1108 66-67 3.6934600134691706 1108 68-69 3.799522549524081 1108 70-71 2.3785811122459073 1108 72-73 1.7909574249706957 1108 74-75 1.5295733313368594 1108 76-77 2.3858545729437246 1108 78-79 2.992216841879049 1108 80-81 3.1895279734839974 1108 82-83 2.353153427675956 1108 84-85 0.2225683265928886 1108 86-87 0.2779459336903294 1108 88-89 0.369674095072952 1108 90-91 0.022799496684800857 1108 92-93 -0.004433561788633966 1108 94-95 0.9916781475244427 1108 96-97 1.6076876491760643 1108 98-99 -0.7129442800460559 1108 100 0.7991997325911377 1109 1 4.132433947627643 1109 2 8.697743546053854 1109 3 4.657327383118055 1109 4 -3.5546297929772024 1109 5 0.448698084220684 1109 6 4.95007276263345 1109 7 2.6007397453688057 1109 8 4.529377538533925 1109 9 3.3647207304168454 1109 10-11 2.2596703742884756 1109 12-13 4.951160132654639 1109 14-15 2.9530333060538183 1109 16-17 3.332882355906431 1109 18-19 3.7631359803296363 1109 20-21 4.588739633912386 1109 22-23 4.77748296086909 1109 24-25 5.269077387774953 1109 26-27 6.862343197420923 1109 28-29 6.1766204130231515 1109 30-31 5.460810396803868 1109 32-33 7.067539451637078 1109 34-35 2.048605011438955 1109 36-37 4.288384777734084 1109 38-39 4.021133930986533 1109 40-41 5.785474618500491 1109 42-43 3.2920626074958896 1109 44-45 4.313085738867127 1109 46-47 4.839208705343825 1109 48-49 3.8024990855276144 1109 50-51 5.44935002893207 1109 52-53 4.64322071870793 1109 54-55 5.016178666324738 1109 56-57 5.574257333218291 1109 58-59 3.8942200911038753 1109 60-61 2.535643763216939 1109 62-63 3.2428190154560426 1109 64-65 4.4325275765316405 1109 66-67 4.16854335293651 1109 68-69 3.0376776334164113 1109 70-71 1.3719278568235787 1109 72-73 1.9020440089798711 1109 74-75 2.5027140118292017 1109 76-77 1.30959703173043 1109 78-79 2.7017396637625737 1109 80-81 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2212 30-31 -6.499737041347554 2212 32-33 -17.23357182949019 2212 34-35 -13.82775299262686 2212 36-37 -17.58410612206429 2212 38-39 -23.62196277108166 2212 40-41 -10.221502011911095 2212 42-43 -14.001412831588189 2212 44-45 -17.108388134680403 2212 46-47 -17.063750720820412 2212 48-49 -17.716962802921664 2212 50-51 -9.052550682325325 2212 52-53 -2.2480492291318086 2212 54-55 -23.40724223920908 2212 56-57 -23.351958429777795 2212 58-59 -12.591521868086001 2212 60-61 -6.471097620346804 2212 62-63 -3.497021495508438 2212 64-65 -17.325901807256937 2212 66-67 -17.490143761469444 2212 68-69 -14.533883520875335 2212 70-71 -9.78875542076874 2212 72-73 -17.369457709050764 2212 74-75 -6.483920532192663 2212 76-77 -10.947514553344096 2212 78-79 -4.996899235448726 2212 80-81 -2.6391549528751455 2212 82-83 -3.2771489357834263 2212 84-85 -5.584906698652251 2212 86-87 -5.409935514261843 2212 88-89 -3.6507515814058564 2212 90-91 -4.567344055282714 2212 92-93 -5.667354390022073 2212 94-95 -4.057276414430831 2212 96-97 -5.7929911587177685 2212 98-99 -4.049469531078618 2212 100 -4.288858056566141 2213 1 -21.117426309499244 2213 2 -17.015504078317303 2213 3 -19.762460207714813 2213 4 -18.793869591747466 2213 5 -21.050463458540634 2213 6 -21.822984811430274 2213 7 -10.686159489190896 2213 8 -10.377037432826521 2213 9 -4.588784392113418 2213 10-11 -17.581241491789378 2213 12-13 -16.290105267422504 2213 14-15 -23.427626280307276 2213 16-17 -17.875637318446266 2213 18-19 -18.50408570446407 2213 20-21 -17.63226604037018 2213 22-23 -19.697814315021503 2213 24-25 -7.853456588822496 2213 26-27 -23.14436446048852 2213 28-29 -24.02293240978289 2213 30-31 -19.421071913914158 2213 32-33 -23.254987099508813 2213 34-35 -17.8554694988422 2213 36-37 -23.636992528023324 2213 38-39 -11.507756129015238 2213 40-41 -17.094268233986725 2213 42-43 -23.195640019670126 2213 44-45 -17.154483704574652 2213 46-47 -16.9797508445088 2213 48-49 -6.498303378897255 2213 50-51 -23.28625645513724 2213 52-53 -17.740441018971495 2213 54-55 -17.66554482223491 2213 56-57 -17.54070381723167 2213 58-59 -17.96162363614279 2213 60-61 -19.12921879373731 2213 62-63 -17.554290936917937 2213 64-65 -17.668431028638537 2213 66-67 -8.896028580094168 2213 68-69 -22.556788548808296 2213 70-71 -17.291483436875716 2213 72-73 -9.416043565970782 2213 74-75 -4.44815053762121 2213 76-77 -3.545048000745613 2213 78-79 -5.129663602198541 2213 80-81 -3.7127041911608245 2213 82-83 -3.7350763306998367 2213 84-85 -3.423281708100671 2213 86-87 -3.710158153359739 2213 88-89 -3.227349738448879 2213 90-91 -3.2687973938384474 2213 92-93 -3.998932722565172 2213 94-95 -4.1126372753020135 2213 96-97 -3.763257501045995 2213 98-99 -4.299421430038951 2213 100 -3.602042551539583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 909.0 3 1166.0 4 9605.0 5 26216.0 6 26696.0 7 32230.0 8 31207.0 9 32582.0 10 37252.0 11 44680.0 12 54295.0 13 70690.0 14 57395.0 15 62277.0 16 69224.0 17 71219.0 18 69636.0 19 69039.0 20 67677.0 21 65463.0 22 68515.0 23 73718.0 24 79478.0 25 86327.0 26 94196.0 27 100612.0 28 108008.0 29 114620.0 30 120566.0 31 132370.0 32 150037.0 33 179677.0 34 215780.0 35 249607.0 36 269946.0 37 231022.0 38 71871.0 39 185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.377406266207025 20.863899990169855 13.827653884092458 23.93103985953066 2 15.049391573775669 21.399388717314523 40.22321563593226 23.328004072977542 3 17.590226295851966 27.341608326779877 29.909116146983678 25.159049230384483 4 12.536211859107413 18.038182587710917 39.0527679677574 30.37283758542428 5 13.14750834204142 38.27784323012149 35.474502046086805 13.100146381750289 6 30.530954998936586 38.41448157657326 18.373036338084166 12.681527086405989 7 25.980820924361307 32.75788161951343 23.93445134667209 17.326846109453175 8 24.544622559723965 36.12254852114145 21.799759053172036 17.533069865962556 9 24.718297386649873 17.236959487825356 22.04017107472712 36.00457205079765 10-11 23.375700026786898 27.655595582915467 29.003068393378545 19.96563599691909 12-13 24.17062641675342 25.324307619102942 29.000362090608917 21.504703873534726 14-15 21.617469456204507 26.655637679156925 26.424313065849383 25.30257979878919 16-17 21.15577090018875 30.2036694422151 27.303936391309374 21.33662326628677 18-19 21.20759258987361 29.301189549794376 28.785266373995416 20.705951486336595 20-21 22.635174539274075 28.608274858164716 27.777916428343325 20.978634174217888 22-23 21.60058649301044 28.56768649595216 28.361799155891653 21.469927855145745 24-25 21.85096309343095 28.359022501458735 27.880225792798235 21.909788612312084 26-27 21.886314528556543 28.344906516821844 27.92631011972474 21.842468834896874 28-29 21.64572339578582 28.26425832775103 28.397518313475196 21.692499962987956 30-31 22.16452601271268 28.676558988559236 27.699338441521824 21.459576557206255 32-33 21.39612677172763 28.500381591668294 28.32503623535525 21.778455401248824 34-35 21.506998712872683 27.97075558196734 28.209132366188054 22.31311333897192 36-37 22.05841726739524 28.48078998081307 27.76970648618919 21.6910862656025 38-39 21.761411538098272 28.267993845701326 28.006773114764655 21.963821501435742 40-41 22.025746522734877 28.23149161514504 27.669885801112333 22.072876061007754 42-43 21.677811349469753 28.407753671442993 28.17421718509755 21.740217793989707 44-45 22.170222548003313 27.912303348312665 28.035216387337552 21.88225771634647 46-47 22.017909761717576 28.37054019380772 28.10145663765931 21.510093406815393 48-49 21.893224217300585 28.086042291145464 28.10016662834921 21.920566863204744 50-51 22.120685491507512 28.18271668112393 27.853294770233017 21.84330305713554 52-53 21.591304111606107 28.249160763094366 27.989561368278643 22.169973757020887 54-55 22.039151718238305 28.21890875598287 27.70321421823831 22.038725307540517 56-57 22.11375768723159 28.173483820373434 27.66740450122182 22.045353991173158 58-59 21.866949801854307 27.717438740850795 28.093966377750245 22.32164507954465 60-61 22.279421231354064 28.108017918128308 27.69500784963975 21.917553000877877 62-63 21.74883433812621 28.231424099197444 28.033785043268654 21.985956519407697 64-65 21.792421156808402 27.82475978834879 27.9705005286085 22.412318526234305 66-67 21.9314281307874 28.04386091252417 27.789056057386773 22.235654899301657 68-69 22.329082663656692 27.8942851524013 27.683623053226448 22.09300913071556 70-71 22.360382594528588 27.934327666026103 27.476354235037576 22.228935504407733 72-73 22.042417686488314 28.084826572039834 27.88223215016787 21.990523591303983 74-75 22.24969136143666 27.73041781930915 27.539341393565586 22.4805494256886 76-77 22.26745414602003 28.058765865558343 27.6428322248855 22.03094776353613 78-79 22.202306833762826 27.78717750530112 27.85176696161941 22.15874869931664 80-81 22.341732561715663 27.825695702843117 27.576244340959427 22.25632739448179 82-83 21.827242804668334 28.007247258186148 27.974563177890595 22.190946759254928 84-85 22.035572781841438 27.683136638360516 27.544207394953663 22.73708318484438 86-87 22.210888982713247 27.880078843427427 27.659003260164265 22.250028913695058 88-89 22.053048555476966 27.49191745547191 27.97825122817123 22.476782760879896 90-91 22.311857662122335 27.628493848995898 27.600103053795532 22.45954543508623 92-93 21.88418571390079 27.84818104475916 27.907784142943378 22.35984909839668 94-95 21.854219730517126 27.435070024880837 27.86825730166343 22.842452942938607 96-97 22.091950925061106 27.6999631118221 27.72249036187675 22.48559560124004 98-99 21.96462932980707 27.4612771037832 27.81953711902178 22.754556447387948 100 22.14009744552755 27.268526474338277 27.595499444842037 22.995876635292138 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 396.0 1 494.5 2 475.5 3 396.5 4 488.0 5 703.0 6 1116.5 7 1763.0 8 2650.5 9 3689.5 10 5023.0 11 6359.5 12 7492.5 13 8482.0 14 9250.5 15 10091.0 16 11046.0 17 12056.0 18 13069.5 19 14039.5 20 15126.0 21 16425.5 22 17805.0 23 19582.0 24 21902.0 25 24937.5 26 29179.5 27 34959.0 28 42303.5 29 50537.5 30 59207.0 31 68215.5 32 77572.0 33 86270.0 34 94357.0 35 101808.0 36 107962.5 37 113003.0 38 116903.5 39 119552.0 40 121082.5 41 120804.0 42 118429.5 43 115040.0 44 110696.5 45 106390.0 46 101960.0 47 97002.5 48 93482.0 49 91742.5 50 90808.0 51 86178.0 52 77785.5 53 70892.0 54 65090.0 55 59828.0 56 55209.0 57 50151.5 58 45160.0 59 40542.0 60 35873.0 61 30962.0 62 26152.5 63 21859.5 64 17970.5 65 14620.5 66 11838.5 67 9589.0 68 7667.5 69 5935.0 70 4547.0 71 3525.0 72 2760.5 73 2127.0 74 1613.5 75 1203.0 76 869.0 77 622.0 78 454.5 79 328.5 80 219.0 81 149.0 82 97.5 83 72.5 84 56.0 85 35.0 86 26.5 87 19.5 88 8.0 89 5.5 90 5.5 91 2.5 92 1.5 93 3.0 94 2.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 9.115330809401007 2 26.661332911069124 3 7.117883495127685 4 24.081259571416204 5 13.943714604436916 6 11.351256764879036 7 9.51751898417526 8 10.62657867715673 9 6.0700377357560535 10-11 7.705669728801016 12-13 11.430154039149192 14-15 8.91996686376095 16-17 9.274496278950695 18-19 7.678051061724409 20-21 9.271107485444361 22-23 6.7945925946235874 24-25 6.178263477462829 26-27 11.117553241796886 28-29 7.400339433880481 30-31 4.93217329797076 32-33 10.12348147392801 34-35 8.13800276217478 36-37 8.278067759234231 38-39 7.202403085896981 40-41 9.107043668917338 42-43 7.612585732624809 44-45 7.6633560207924045 46-47 6.9716416517226 48-49 4.79306332453582 50-51 7.766868258804009 52-53 5.305572131548035 54-55 6.0779243824617 56-57 5.894452021307502 58-59 4.525102179826019 60-61 5.003461190458513 62-63 3.481492412337303 64-65 5.120436180854364 66-67 3.318214179759476 68-69 6.165139604429215 70-71 3.146725824732216 72-73 1.9874811806433346 74-75 1.0693954053505352 76-77 1.4456285025876519 78-79 2.1496657571350277 80-81 2.5338933263257193 82-83 1.218086422244287 84-85 1.710416504286978 86-87 0.5099980190961595 88-89 0.7370933948409624 90-91 0.3323944321506547 92-93 0.5278507994317918 94-95 0.7590281309910404 96-97 0.7840127812968174 98-99 0.4737687357920981 100 0.8622939112930929 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3245993.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.61856283239725 #Duplication Level Percentage of deduplicated Percentage of total 1 96.31438425796807 90.16814234318234 2 2.7635696976385087 5.174428467601596 3 0.49490123160770577 1.3899582614129036 4 0.1679940448898414 0.6290944418795272 5 0.08176390907359239 0.3827309829514262 6 0.044415738085892595 0.24948825400448613 7 0.03500960820115437 0.22942844435821655 8 0.021965100695121587 0.16450729276369383 9 0.014900814196469332 0.1255493529095437 >10 0.058200281187783955 0.9109780456650921 >50 0.0013247530760171682 0.0845701986046454 >100 0.0013386769611302314 0.25226428479874824 >500 1.656331569056173E-4 0.1069627198738368 >1k 6.625326178605485E-5 0.1318969099938626 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.080721369392972E-5 0.0 5 0.0 0.0 0.0 3.080721369392972E-5 0.0 6 0.0 0.0 0.0 3.080721369392972E-5 0.0 7 0.0 0.0 0.0 6.161442738785943E-5 0.0 8 0.0 0.0 0.0 6.161442738785943E-5 0.0 9 0.0 0.0 0.0 9.242164108178915E-5 0.0 10-11 0.0 0.0 0.0 9.242164108178915E-5 0.0 12-13 0.0 0.0 1.540360684696486E-5 9.242164108178915E-5 0.0 14-15 0.0 0.0 3.080721369392972E-5 9.242164108178915E-5 0.0 16-17 0.0 0.0 3.080721369392972E-5 1.3863246162268373E-4 0.0 18-19 0.0 0.0 3.080721369392972E-5 1.5403606846964857E-4 0.0 20-21 0.0 0.0 3.080721369392972E-5 1.5403606846964857E-4 0.0 22-23 0.0 0.0 3.080721369392972E-5 1.6943967531661344E-4 0.0 24-25 3.080721369392972E-5 0.0 4.6210820540894575E-5 2.15650495857508E-4 0.0 26-27 3.080721369392972E-5 0.0 7.70180342348243E-5 2.6186131639840255E-4 0.0 28-29 3.080721369392972E-5 0.0 9.242164108178915E-5 6.777587012664537E-4 0.0 30-31 6.161442738785943E-5 0.0 9.242164108178915E-5 0.002464577095514377 0.0 32-33 7.70180342348243E-5 0.0 9.242164108178915E-5 0.005868774208693611 0.0 34-35 9.242164108178915E-5 0.0 9.242164108178915E-5 0.012292078263877956 0.0 36-37 9.242164108178915E-5 0.0 9.242164108178915E-5 0.022088772218547607 0.0 38-39 9.242164108178915E-5 0.0 9.242164108178915E-5 0.03749237906551246 0.0 40-41 9.242164108178915E-5 0.0 9.242164108178915E-5 0.05630018302565656 0.0 42-43 9.242164108178915E-5 0.0 9.242164108178915E-5 0.07498475813102493 0.0 44-45 9.242164108178915E-5 0.0 9.242164108178915E-5 0.09699651231533771 0.0 46-47 9.242164108178915E-5 0.0 9.242164108178915E-5 0.12635578696565272 0.0 48-49 9.242164108178915E-5 0.0 1.2322885477571887E-4 0.15734784394174603 0.0 50-51 9.242164108178915E-5 0.0 1.2322885477571887E-4 0.19086609244074154 0.0 52-53 1.07825247928754E-4 0.0 1.2322885477571887E-4 0.22433813011919618 0.0 54-55 1.2322885477571887E-4 3.080721369392972E-5 1.3863246162268373E-4 0.2634632915104869 0.0 56-57 1.2322885477571887E-4 3.080721369392972E-5 1.848432821635783E-4 0.31201546029212013 0.0 58-59 1.2322885477571887E-4 3.080721369392972E-5 1.848432821635783E-4 0.36454175964027036 0.0 60-61 1.2322885477571887E-4 3.080721369392972E-5 1.848432821635783E-4 0.42144268333295853 0.0 62-63 1.2322885477571887E-4 3.080721369392972E-5 2.15650495857508E-4 0.48136271396765185 0.0 64-65 1.2322885477571887E-4 6.161442738785943E-5 2.15650495857508E-4 0.5502168365735847 0.0 66-67 1.2322885477571887E-4 9.242164108178915E-5 2.15650495857508E-4 0.6308855256311396 0.0 68-69 1.3863246162268373E-4 9.242164108178915E-5 2.15650495857508E-4 0.724970756252401 0.0 70-71 1.5403606846964857E-4 9.242164108178915E-5 2.15650495857508E-4 0.834998719960271 0.0 72-73 1.5403606846964857E-4 9.242164108178915E-5 2.15650495857508E-4 0.950741421808365 0.0 74-75 1.5403606846964857E-4 9.242164108178915E-5 2.15650495857508E-4 1.0726763736089389 0.0 76-77 1.5403606846964857E-4 9.242164108178915E-5 2.15650495857508E-4 1.2076735840157387 0.0 78-79 1.5403606846964857E-4 1.07825247928754E-4 2.15650495857508E-4 1.361817477733316 0.0 80-81 1.5403606846964857E-4 1.2322885477571887E-4 2.15650495857508E-4 1.5369872947970005 0.0 82-83 1.5403606846964857E-4 1.3863246162268373E-4 2.15650495857508E-4 1.7297942417004597 0.0 84-85 1.5403606846964857E-4 1.5403606846964857E-4 2.15650495857508E-4 1.9263134578540373 0.0 86-87 1.5403606846964857E-4 1.5403606846964857E-4 2.15650495857508E-4 2.1435813324304767 0.0 88 1.5403606846964857E-4 1.5403606846964857E-4 2.15650495857508E-4 2.3224326115305858 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATA 65 0.00775823 18.190205 50-51 GTATATA 1735 0.0 18.041607 1 GTACATG 11915 0.0 17.823238 1 TACATGG 11975 0.0 17.320744 2 GTGTATA 1505 0.0 17.128422 1 GTCTATA 975 0.0 16.996664 1 CATGGGG 5115 0.0 16.586727 4 ACATGGG 11820 0.0 16.372458 3 GTATAGG 800 0.0 16.111422 1 GTATTAG 1135 0.0 15.682188 1 GTATCAA 8405 0.0 15.481131 1 TAGACCG 230 5.228363E-4 15.288059 5 TAGTACG 300 1.1023022E-4 14.787121 4 ACACCGT 385 6.932005E-7 14.351788 6 GTATTAC 1135 0.0 14.059893 1 GTGTAGA 1415 0.0 13.880275 1 CTACACT 1280 0.0 13.862926 4 TATCAAC 9255 0.0 13.658915 2 GTGTAAG 1180 0.0 13.523711 1 GTATTAT 1455 0.0 13.498687 1 >>END_MODULE