FastQCFastQC Report
Wed 25 May 2016
SRR1295122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3245993
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT66530.2049603927057144No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47520.146395879473554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC6900.018.3941843
GTACCGA1600.001288546117.6060266
ACCGCGT1650.001584654917.0706678
CATGGGG55950.016.8046424
GTATCAA122400.016.3881151
GAGTACT44000.016.37666712-13
GTACCGG2454.6146757E-516.3747371
TACACCG2951.3721965E-615.9361735
GTACATG154350.015.9198851
CAACGCA133950.015.5126375
ACATGGG150550.015.39331153
TACATGG157400.015.3123072
TCAACGC134400.015.2855664
ATCAACG134850.015.2682423
AACGCAG135150.015.2503046
CCTAGTA10050.014.9573762
TATCAAC136000.014.884792
GTATATA21400.014.5287531
GTATAGA13600.014.3805721
CGCAGAG146000.014.05114658
Produced by FastQC (version 0.11.5)