##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295122_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3245993 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.155041615924617 33.0 33.0 33.0 27.0 33.0 2 29.658727853079167 33.0 33.0 33.0 22.0 33.0 3 30.60049883040413 33.0 33.0 33.0 22.0 33.0 4 34.930527268543095 37.0 37.0 37.0 33.0 37.0 5 35.287627237643456 37.0 37.0 37.0 33.0 37.0 6 35.00754253012868 37.0 37.0 37.0 33.0 37.0 7 34.896414440819804 37.0 37.0 37.0 33.0 37.0 8 34.874164854945775 37.0 37.0 37.0 33.0 37.0 9 34.83572330562635 37.0 37.0 37.0 33.0 37.0 10-11 34.81132029551512 37.0 37.0 37.0 30.0 37.0 12-13 34.71585628804498 37.0 37.0 37.0 27.0 37.0 14-15 36.10810282092414 37.0 37.0 40.0 27.0 40.0 16-17 36.09971971596981 37.0 37.0 40.0 27.0 40.0 18-19 36.12210670201692 37.0 37.0 40.0 27.0 40.0 20-21 36.12067924977041 37.0 37.0 40.0 27.0 40.0 22-23 36.074715040975136 37.0 37.0 40.0 27.0 40.0 24-25 36.042111766722854 37.0 37.0 40.0 27.0 40.0 26-27 36.01390683836965 37.0 37.0 40.0 27.0 40.0 28-29 35.95890594958153 37.0 37.0 40.0 27.0 40.0 30-31 35.86719718742462 37.0 37.0 40.0 27.0 40.0 32-33 35.82039332802012 37.0 37.0 40.0 27.0 40.0 34-35 35.72205069450242 37.0 37.0 40.0 27.0 40.0 36-37 35.713153417151545 37.0 37.0 40.0 27.0 40.0 38-39 35.647959037496385 37.0 37.0 40.0 27.0 40.0 40-41 35.58604439381108 37.0 37.0 40.0 27.0 40.0 42-43 35.48544143502466 37.0 37.0 40.0 27.0 40.0 44-45 35.36995874606014 37.0 35.0 40.0 27.0 40.0 46-47 35.261373022061356 37.0 33.0 40.0 27.0 40.0 48-49 35.1526703846866 37.0 33.0 40.0 27.0 40.0 50-51 35.04281786806071 37.0 33.0 40.0 27.0 40.0 52-53 34.94356472734229 37.0 33.0 40.0 27.0 40.0 54-55 34.81956969716201 37.0 33.0 40.0 24.5 40.0 56-57 34.68881186743163 37.0 33.0 40.0 22.0 40.0 58-59 34.557101478653834 37.0 33.0 40.0 22.0 40.0 60-61 34.394636248445394 37.0 33.0 40.0 22.0 40.0 62-63 34.21511722298847 37.0 33.0 40.0 22.0 40.0 64-65 34.025126363488766 37.0 33.0 40.0 22.0 40.0 66-67 33.80106642250923 37.0 33.0 38.5 22.0 40.0 68-69 33.58775018923331 37.0 33.0 37.0 22.0 40.0 70-71 33.358317778257685 37.0 33.0 37.0 22.0 40.0 72-73 33.14746720032976 37.0 33.0 37.0 22.0 40.0 74-75 32.91451907012738 37.0 33.0 37.0 22.0 40.0 76-77 32.68187685555699 37.0 33.0 37.0 22.0 37.0 78-79 32.45910604243447 37.0 33.0 37.0 22.0 37.0 80-81 32.255059545722986 37.0 33.0 37.0 22.0 37.0 82-83 32.04966631166487 33.0 33.0 37.0 22.0 37.0 84-85 31.881027161796098 33.0 33.0 37.0 22.0 37.0 86-87 31.911838534463875 33.0 33.0 37.0 22.0 37.0 88-89 31.928506007252636 33.0 33.0 37.0 22.0 37.0 90-91 31.944905303246188 33.0 33.0 37.0 22.0 37.0 92-93 31.922131532631155 33.0 33.0 37.0 22.0 37.0 94-95 31.954829847137688 33.0 33.0 37.0 22.0 37.0 96-97 31.911945589531463 33.0 33.0 37.0 22.0 37.0 98-99 31.837405841602248 33.0 33.0 37.0 22.0 37.0 100 31.300462139012623 33.0 27.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7217275146741073 1101 2 4.267956832108425 1101 3 2.4551287663405255 1101 4 1.296682587515761 1101 5 0.5699287556236357 1101 6 0.5692627423147698 1101 7 0.6615538643847998 1101 8 0.5848999689885019 1101 9 0.604146425785153 1101 10-11 0.7069073719544008 1101 12-13 0.7229509958549301 1101 14-15 0.9601896516084523 1101 16-17 0.9953821845163446 1101 18-19 1.0059718168550233 1101 20-21 1.0080279280508364 1101 22-23 0.9342718476783674 1101 24-25 0.9496965681734792 1101 26-27 0.9808548465146458 1101 28-29 0.8327170054464759 1101 30-31 0.7573730177583045 1101 32-33 0.9810265815541541 1101 34-35 1.1950589840212515 1101 36-37 0.9875418855603968 1101 38-39 0.9793636641713306 1101 40-41 1.001025663286704 1101 42-43 1.0946659847416882 1101 44-45 1.0810981802591044 1101 46-47 1.1406975441117027 1101 48-49 1.0397163104077123 1101 50-51 0.9281674259511163 1101 52-53 0.9950429849670783 1101 54-55 0.9408699288704128 1101 56-57 0.8967298384382616 1101 58-59 0.9396650987974766 1101 60-61 0.8924856348275014 1101 62-63 0.9865027582949182 1101 64-65 0.915406046995848 1101 66-67 0.9361202724712783 1101 68-69 0.8785348407381761 1101 70-71 0.8796036594198569 1101 72-73 0.8127059195235589 1101 74-75 0.808696818422618 1101 76-77 0.8773429541409143 1101 78-79 0.9034541374091845 1101 80-81 0.8205940343073408 1101 82-83 0.80716223586154 1101 84-85 0.820403991574306 1101 86-87 0.8577904609740337 1101 88-89 0.7848548086428089 1101 90-91 0.8231440330271447 1101 92-93 0.8823622092908217 1101 94-95 0.8505208856771596 1101 96-97 0.8331640470346677 1101 98-99 0.8349254636380401 1101 100 0.8917685861778253 1102 1 0.606157443813025 1102 2 4.094741285911688 1102 3 2.3243377227915616 1102 4 1.1160156346032082 1102 5 0.6054038172121778 1102 6 0.5802159106608471 1102 7 0.5226841702111216 1102 8 0.53418560279475 1102 9 0.6625884564256452 1102 10-11 0.6300823260484805 1102 12-13 0.6461391050790439 1102 14-15 0.8645143397070214 1102 16-17 0.9150698719845565 1102 18-19 0.8961122295972288 1102 20-21 0.8499079544829087 1102 22-23 0.8811848989681508 1102 24-25 0.8863288853339242 1102 26-27 0.9121910362164911 1102 28-29 0.9106664800025683 1102 30-31 0.9009137270999616 1102 32-33 1.0085540870430734 1102 34-35 1.0890283922535602 1102 36-37 0.9503185620020602 1102 38-39 0.9391210251060755 1102 40-41 0.9405993091761786 1102 42-43 1.0644106473217434 1102 44-45 0.9898456718011488 1102 46-47 0.9232263437974453 1102 48-49 0.9322754399766069 1102 50-51 0.8144958656978858 1102 52-53 0.8603149642355632 1102 54-55 0.9421832798048229 1102 56-57 0.8806447990489943 1102 58-59 0.9661800855966689 1102 60-61 0.8363732193690012 1102 62-63 0.9515923014891214 1102 64-65 0.8503200666493669 1102 66-67 0.8270294990288107 1102 68-69 0.8137296554244173 1102 70-71 0.8932023183274325 1102 72-73 0.8134137690556642 1102 74-75 0.8278858290167577 1102 76-77 0.7764644837902068 1102 78-79 0.7869156114536864 1102 80-81 0.7184110744639796 1102 82-83 0.8634208194316315 1102 84-85 0.8644958460517707 1102 86-87 0.8762438500948626 1102 88-89 0.748242772307858 1102 90-91 0.7587205291948003 1102 92-93 0.8213686011005414 1102 94-95 0.848677583983136 1102 96-97 0.8136062664756416 1102 98-99 0.8040749479386697 1102 100 0.8376399765106335 1103 1 0.5526504524626752 1103 2 4.061165456983478 1103 3 2.2759417512517537 1103 4 1.0928537522546833 1103 5 0.450657014941072 1103 6 0.4684916442139553 1103 7 0.47041292478748886 1103 8 0.4626552167394635 1103 9 0.44827608450284373 1103 10-11 0.5568764328330715 1103 12-13 0.5432146923659502 1103 14-15 0.7647819691866289 1103 16-17 0.7389644587797548 1103 18-19 0.7595736065641887 1103 20-21 0.7408490999295552 1103 22-23 0.6866984267635203 1103 24-25 0.7198307963613999 1103 26-27 0.8117301856550156 1103 28-29 0.7390547654540924 1103 30-31 0.8229825184484767 1103 32-33 0.846852180107156 1103 34-35 0.903841886586811 1103 36-37 0.7474243168993482 1103 38-39 0.7764534828204788 1103 40-41 0.7552571831115387 1103 42-43 0.7798904123679478 1103 44-45 0.7722343599916002 1103 46-47 0.7800116475911665 1103 48-49 0.7537483918966075 1103 50-51 0.7259355253370998 1103 52-53 0.6985890842578542 1103 54-55 0.6593472481145497 1103 56-57 0.6960467978366154 1103 58-59 0.6514138731322774 1103 60-61 0.6272326480123311 1103 62-63 0.7276608334055865 1103 64-65 0.7025153810907625 1103 66-67 0.636010319593943 1103 68-69 0.60578935071009 1103 70-71 0.694023669891557 1103 72-73 0.740115601088597 1103 74-75 0.7480685162664003 1103 76-77 0.601804851423033 1103 78-79 0.6614354787390937 1103 80-81 0.7044028781361718 1103 82-83 0.7538850689330729 1103 84-85 0.6919676071676228 1103 86-87 0.7124522725785774 1103 88-89 0.7102824824968827 1103 90-91 0.7731145218652564 1103 92-93 0.7700517709072763 1103 94-95 0.6774084100714681 1103 96-97 0.68635268047748 1103 98-99 0.7170440794693498 1103 100 0.7812235816559543 1104 1 0.4012043298288539 1104 2 3.575200763565956 1104 3 2.080179524232335 1104 4 0.9838866543276694 1104 5 0.3181359281695677 1104 6 0.3523048165124578 1104 7 0.29012847153262555 1104 8 0.27260775186524455 1104 9 0.3469440586286794 1104 10-11 0.4195558986621677 1104 12-13 0.4032244858655929 1104 14-15 0.48032253376052836 1104 16-17 0.528533500204162 1104 18-19 0.5634992817416844 1104 20-21 0.5052673785985888 1104 22-23 0.4434566311268142 1104 24-25 0.4604082602577222 1104 26-27 0.5218205598291306 1104 28-29 0.4586174365408979 1104 30-31 0.421944318303737 1104 32-33 0.5365192498586779 1104 34-35 0.7210439343449551 1104 36-37 0.5928891560682885 1104 38-39 0.5631715103988668 1104 40-41 0.4679866793716272 1104 42-43 0.5154138331324205 1104 44-45 0.5833012427291706 1104 46-47 0.6029659660588962 1104 48-49 0.45318994257137746 1104 50-51 0.4597317270064494 1104 52-53 0.5020702876138969 1104 54-55 0.5341901285026651 1104 56-57 0.5240857926402782 1104 58-59 0.524153888797322 1104 60-61 0.4752835766992831 1104 62-63 0.5467229409306213 1104 64-65 0.5155231687066291 1104 66-67 0.4827679338999218 1104 68-69 0.43935608334081167 1104 70-71 0.5138586695405323 1104 72-73 0.48789017762396014 1104 74-75 0.511709225907282 1104 76-77 0.4980914205881817 1104 78-79 0.4680631394024175 1104 80-81 0.4454729724862361 1104 82-83 0.6115148989125743 1104 84-85 0.5013690868322662 1104 86-87 0.5384652241811274 1104 88-89 0.42453419182240637 1104 90-91 0.41261744887102125 1104 92-93 0.4648954684573745 1104 94-95 0.47117757073100464 1104 96-97 0.4168016186905952 1104 98-99 0.4726721840756234 1104 100 0.36634884854282035 1105 1 0.41563121638281686 1105 2 3.7568265690686538 1105 3 2.16883675927825 1105 4 0.9815489728884756 1105 5 0.3672612510600928 1105 6 0.38974632851341084 1105 7 0.2867943108641384 1105 8 0.30026280826192675 1105 9 0.3625705811551754 1105 10-11 0.3415469266720592 1105 12-13 0.34360395692264945 1105 14-15 0.42733631348713885 1105 16-17 0.454339002902195 1105 18-19 0.5088000180699765 1105 20-21 0.46827630462563263 1105 22-23 0.39879110434413434 1105 24-25 0.5345770182438017 1105 26-27 0.441407259956911 1105 28-29 0.4023462360775909 1105 30-31 0.4323788531115653 1105 32-33 0.5117097667592887 1105 34-35 0.6548387368416684 1105 36-37 0.4991994251360694 1105 38-39 0.4657008578152855 1105 40-41 0.4652040754721227 1105 42-43 0.5264982856663707 1105 44-45 0.5940418915437817 1105 46-47 0.5480575704456072 1105 48-49 0.42664811158675775 1105 50-51 0.5053087013498683 1105 52-53 0.3900560022935835 1105 54-55 0.4808067873556112 1105 56-57 0.3607960322916881 1105 58-59 0.4066920611220368 1105 60-61 0.479453345872777 1105 62-63 0.47576929927705436 1105 64-65 0.3696865030511489 1105 66-67 0.44945390505350247 1105 68-69 0.43586105194804503 1105 70-71 0.42728448055879653 1105 72-73 0.516962734916909 1105 74-75 0.44175574052813005 1105 76-77 0.37121313997447203 1105 78-79 0.3901971125415997 1105 80-81 0.47075173846989316 1105 82-83 0.4559427966539431 1105 84-85 0.48416465231585093 1105 86-87 0.4809424494828711 1105 88-89 0.477441815256217 1105 90-91 0.4436452749100219 1105 92-93 0.517709786871098 1105 94-95 0.4416807743565734 1105 96-97 0.42820459525863086 1105 98-99 0.45565114218488745 1105 100 0.4899665093905625 1106 1 0.518815170856918 1106 2 3.916669395172061 1106 3 2.2248433220030783 1106 4 1.0165288727420645 1106 5 0.2915607486465106 1106 6 0.18530952296536185 1106 7 0.1520770881554796 1106 8 0.1523790588129259 1106 9 0.23762802301368424 1106 10-11 0.20656366378231894 1106 12-13 0.24070832274411202 1106 14-15 0.15879979872160277 1106 16-17 0.15939112395707866 1106 18-19 0.2029767408152665 1106 20-21 0.19741785361606645 1106 22-23 0.13794495243819682 1106 24-25 0.11159523028563001 1106 26-27 0.1279782362342914 1106 28-29 0.17815885971420897 1106 30-31 0.155207262599653 1106 32-33 0.2337930235234964 1106 34-35 0.3468646178070074 1106 36-37 0.13633863441420147 1106 38-39 0.20487704607804602 1106 40-41 0.08718800256149706 1106 42-43 0.22112043053876107 1106 44-45 0.20799656982733694 1106 46-47 0.09264290208300707 1106 48-49 0.023514482546460158 1106 50-51 0.04089304689703255 1106 52-53 -0.005229861784506795 1106 54-55 0.05309873252371489 1106 56-57 0.10573862646570831 1106 58-59 0.14761774883290713 1106 60-61 0.12160089668164886 1106 62-63 0.17361149120792874 1106 64-65 0.06484048806881759 1106 66-67 -0.00991173024204528 1106 68-69 0.07243809370133647 1106 70-71 0.04878893478007029 1106 72-73 0.020177256987437886 1106 74-75 -0.008200359839278804 1106 76-77 0.08637130476564892 1106 78-79 0.10689891092518167 1106 80-81 0.042534183661409486 1106 82-83 0.010730138666318112 1106 84-85 0.11121877677941328 1106 86-87 0.22156156041622665 1106 88-89 0.1263553143632059 1106 90-91 0.06611221806630141 1106 92-93 0.17495706971805092 1106 94-95 0.15056945906112773 1106 96-97 0.11977160195226944 1106 98-99 0.135943990568407 1106 100 0.11663694746205167 1107 1 0.5046798703158188 1107 2 3.639579074827328 1107 3 2.1401431227013568 1107 4 1.0490509441162956 1107 5 0.33760365049130314 1107 6 0.23001125326572947 1107 7 0.11653158727316537 1107 8 0.15485760339374366 1107 9 0.19821461719634925 1107 10-11 0.1777428548335962 1107 12-13 0.21835529286223476 1107 14-15 0.17913129339250133 1107 16-17 0.11815997097915698 1107 18-19 0.1977815520830859 1107 20-21 0.28676988353830524 1107 22-23 0.24880939568489424 1107 24-25 0.2471616673652477 1107 26-27 0.2621448866916154 1107 28-29 0.16388125159422628 1107 30-31 0.15507344662526634 1107 32-33 0.2599916208191928 1107 34-35 0.3378502149163509 1107 36-37 0.23164707521548422 1107 38-39 0.12874506395559138 1107 40-41 0.2078758099769189 1107 42-43 0.3257821331723889 1107 44-45 0.3537107252692877 1107 46-47 0.19393462990073118 1107 48-49 0.16682664333980313 1107 50-51 0.20933630990690943 1107 52-53 0.10018521976522976 1107 54-55 0.1728917005130839 1107 56-57 0.07936489505599553 1107 58-59 0.2177140406998106 1107 60-61 0.25348635915931794 1107 62-63 0.2768582540974407 1107 64-65 0.15646325878734757 1107 66-67 0.20319345082886997 1107 68-69 0.13606142996908233 1107 70-71 0.21626761635598513 1107 72-73 0.1876687910041639 1107 74-75 0.15248939897196578 1107 76-77 0.1693533136492107 1107 78-79 0.22591452062761164 1107 80-81 0.21199199471400476 1107 82-83 0.2976635881212957 1107 84-85 0.34222524315715575 1107 86-87 0.2957965682897843 1107 88-89 0.15888876365086446 1107 90-91 0.31185420129098773 1107 92-93 0.3966869392296992 1107 94-95 0.23514379019563947 1107 96-97 0.28198015534051635 1107 98-99 0.2652326011724959 1107 100 0.21068636148905284 1108 1 0.47042024996258647 1108 2 3.702499672821478 1108 3 2.125241858155743 1108 4 0.9736770287655219 1108 5 0.23374129156383106 1108 6 0.13706032713363214 1108 7 0.012312921314070024 1108 8 -0.03315063480678049 1108 9 -0.08132223436435737 1108 10-11 0.07402448907151893 1108 12-13 0.06784940490227598 1108 14-15 0.017567454943701932 1108 16-17 0.025085078900090707 1108 18-19 0.06584051534073865 1108 20-21 0.12625583681066388 1108 22-23 0.021820247141661753 1108 24-25 0.032790300203380696 1108 26-27 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2.4108108456622084 2212 4 1.1955280251321483 2212 5 0.6991739713611835 2212 6 0.5839978744515406 2212 7 0.5794555245139676 2212 8 0.5043722143231051 2212 9 0.3871853734455186 2212 10-11 0.41006518327814945 2212 12-13 0.3571052808899964 2212 14-15 0.4904396615137614 2212 16-17 0.48567577776488946 2212 18-19 0.4249590984430398 2212 20-21 0.5461570139892657 2212 22-23 0.3713307644341839 2212 24-25 0.36990140988670106 2212 26-27 0.29523576493455295 2212 28-29 0.2507268315093114 2212 30-31 0.15520137862838368 2212 32-33 0.3089765795730912 2212 34-35 0.34497459082025017 2212 36-37 0.34912792304208295 2212 38-39 0.33168982606363784 2212 40-41 0.4160837083756519 2212 42-43 0.29165397951168615 2212 44-45 0.4509614870462215 2212 46-47 0.5681213723140885 2212 48-49 0.3776338190253341 2212 50-51 0.3161031603304494 2212 52-53 0.3944486666501845 2212 54-55 0.26952359977655505 2212 56-57 0.4410877765499137 2212 58-59 0.40349607466814774 2212 60-61 0.4242301571936835 2212 62-63 0.3910310676574156 2212 64-65 0.3772024731617094 2212 66-67 0.31210545413458135 2212 68-69 0.38449347618331586 2212 70-71 0.3004979103806704 2212 72-73 0.43282755667240735 2212 74-75 0.448106462227841 2212 76-77 0.4051499195515902 2212 78-79 0.41811121574033905 2212 80-81 0.34528756416192863 2212 82-83 0.4620534756095438 2212 84-85 0.44564191700716904 2212 86-87 0.41652456295905793 2212 88-89 0.4500836211948105 2212 90-91 0.4546361682147513 2212 92-93 0.38320099054206835 2212 94-95 0.4849509344112626 2212 96-97 0.36432376017702595 2212 98-99 0.44580663959588307 2212 100 0.4703081479671205 2213 1 -0.7482853088583852 2213 2 -25.60097933810434 2213 3 -14.633020196000164 2213 4 -6.726171041020525 2213 5 -2.631471888170033 2213 6 -1.6677004358276477 2213 7 -1.6713769670511738 2213 8 -1.631565446729482 2213 9 -1.4388888258439607 2213 10-11 -1.6546953079021876 2213 12-13 -1.6596239171282434 2213 14-15 -1.8680477669359448 2213 16-17 -1.8539666940788209 2213 18-19 -1.8348088483984384 2213 20-21 -1.8929453518947312 2213 22-23 -1.8654606900725383 2213 24-25 -1.7552849816420206 2213 26-27 -1.9087518112540565 2213 28-29 -1.794509447669121 2213 30-31 -1.9503457019820942 2213 32-33 -2.021699858476204 2213 34-35 -1.800745103806861 2213 36-37 -1.8905107694342362 2213 38-39 -1.896597708208354 2213 40-41 -1.9782420868376036 2213 42-43 -1.9111891355116413 2213 44-45 -1.8038297200837334 2213 46-47 -1.9149683636112016 2213 48-49 -1.9253474146976188 2213 50-51 -1.875025633639055 2213 52-53 -2.061750870205522 2213 54-55 -1.8842691672013956 2213 56-57 -1.9568751444200814 2213 58-59 -1.9136423257661335 2213 60-61 -1.8204752926541161 2213 62-63 -1.8354121353221018 2213 64-65 -1.9097707345591992 2213 66-67 -1.9288934670278195 2213 68-69 -1.7763103609053275 2213 70-71 -1.852906612491374 2213 72-73 -1.792055798306393 2213 74-75 -1.8690546144108637 2213 76-77 -1.9135541697239375 2213 78-79 -1.8896127804940335 2213 80-81 -2.0035532007820365 2213 82-83 -1.8985651724763777 2213 84-85 -1.9227584485607316 2213 86-87 -1.754818211921208 2213 88-89 -2.0150486223063915 2213 90-91 -1.7604661839260878 2213 92-93 -1.8292881008292987 2213 94-95 -1.9558576440655848 2213 96-97 -1.966308356199999 2213 98-99 -1.8656929446737038 2213 100 -1.9091698870366933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 42.0 5 49.0 6 46.0 7 41.0 8 50.0 9 102.0 10 577.0 11 2589.0 12 7532.0 13 13826.0 14 17696.0 15 20080.0 16 21289.0 17 21887.0 18 22160.0 19 23332.0 20 25340.0 21 28159.0 22 30580.0 23 32693.0 24 34147.0 25 35960.0 26 38751.0 27 44556.0 28 51429.0 29 60937.0 30 72927.0 31 89462.0 32 111927.0 33 148863.0 34 201070.0 35 283525.0 36 425472.0 37 707339.0 38 666721.0 39 4820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.417594946230345 20.55247942181533 10.410438506024725 30.619487125929602 2 12.830223754367456 22.651393677755085 36.09754463361971 28.420837934257744 3 15.12329200956379 27.248025488656324 28.836106547364704 28.792575954415184 4 10.499635377979171 18.695327877918324 37.33162611562477 33.47341062847774 5 11.742004999276428 38.57647439621904 34.96352367220027 14.717996932304267 6 29.790573463268366 39.616211631026594 17.691709244062178 12.901505661642862 7 25.925019654057767 33.286130722366366 23.260450242027662 17.52839938154821 8 24.689049953877966 36.557160349658346 20.78897188697836 17.96481780948533 9 24.79378628233435 16.721094020634318 21.784286459238658 36.70083323779268 10-11 23.15731905428641 27.656833795579878 28.946953163982307 20.238893986151403 12-13 23.859930499042516 25.271785233997495 29.274138607734113 21.594145659225873 14-15 21.592036304971497 26.52015664678609 26.359521725857643 25.528285322384765 16-17 20.97690341184434 30.099572670051757 27.246494632450478 21.677029285653422 18-19 21.002373580454826 29.055213287120036 29.185733248384675 20.756679884040462 20-21 22.170096642458034 28.31265343527069 28.39486562097779 21.122384301293486 22-23 21.36266746578271 28.270049865149165 28.675549899430013 21.691732769638108 24-25 21.508923855884703 28.076436225906264 28.485945720425494 21.928694197783532 26-27 21.414659043344205 28.17677591878028 28.49231405364826 21.916250984227254 28-29 21.16731576257741 28.06559353105145 28.957005914350155 21.81008479202098 30-31 21.83517151434428 28.41204117052984 28.277168474547288 21.475618840578594 32-33 20.91217742552881 28.413510245810414 28.91863411376039 21.755678214900385 34-35 21.12842421519151 27.910639715712716 28.985851719638315 21.975084349457457 36-37 21.713231796436556 28.27435234182254 28.355286507562894 21.657129354178014 38-39 21.435343959748344 28.30888543106517 28.617794277152836 21.637976332033645 40-41 21.605059512118473 28.102012852122034 28.415590457418777 21.877337178340717 42-43 21.290028996321812 28.2461885233843 28.6295968289673 21.834185651326585 44-45 21.675212263758556 27.81884546015283 28.511386974747715 21.994555301340903 46-47 21.694381891607044 28.330460362008253 28.341429468720868 21.633728277663835 48-49 21.544675175284752 28.168957446483972 28.43994065732688 21.846426720904393 50-51 21.754042256986583 28.14694561489436 28.076659560513413 22.022352567605648 52-53 21.311462275321595 28.370559338426226 28.109505053834067 22.20847333241811 54-55 21.666337208308022 28.227884318054326 27.920740792897135 22.18503768074052 56-57 21.72753776623082 28.133608093934605 27.94072516586083 22.198128973973745 58-59 21.36566384344206 27.802073658138966 28.49253301589067 22.339729482528302 60-61 21.85972980962999 28.134485436516293 27.891741451235298 22.114043302618423 62-63 21.284872219928392 28.365758515076635 28.235374774297316 22.11399449069766 64-65 21.314477926901585 27.843559637137833 28.343349414218345 22.49861302174224 66-67 21.568869124505756 28.1075596300423 28.029463132413696 22.29410811303825 68-69 21.66210672106245 28.006278380249388 28.08136070810663 22.250254190581536 70-71 21.74438247778044 27.941052302014658 27.87518404592258 22.43938117428232 72-73 21.438272490379333 28.176292163274276 28.185505695827395 22.199929650519003 74-75 21.78272876640737 27.701707882563355 27.979565436578714 22.535997914450565 76-77 21.630342913363144 28.075024411497267 28.07297535555742 22.221657319582167 78-79 21.591265352164058 27.999839162486406 28.203135314726204 22.205760170623336 80-81 21.68010474599881 27.89339793019121 27.9300171264453 22.496480197364683 82-83 21.162408298664676 28.142431572341113 28.196933999686358 22.498226129307856 84-85 21.347561651939216 27.71513476540836 28.018588013458057 22.918715569194365 86-87 21.49438797674469 27.982690998271565 27.9182983076122 22.604622717371544 88-89 21.250722674582235 27.661664595500184 28.2987119639788 22.788900765938777 90-91 21.47246181773816 27.722977877518524 28.040346940058193 22.76421336468512 92-93 20.941042399267005 28.0567572580193 28.252175541875214 22.750024800838485 94-95 20.957521730209596 27.5503998531089 28.413477505143348 23.07860091153816 96-97 21.224313061255547 27.843728155531235 28.097951945788598 22.834006837424624 98-99 21.043608241483515 27.672260579198383 28.21736744867249 23.066763730645608 100 21.1914953985838 27.494016925316423 28.11912486738546 23.195362808714318 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 56.0 1 53.0 2 42.5 3 36.5 4 41.5 5 56.5 6 107.0 7 183.0 8 274.5 9 328.5 10 295.5 11 262.5 12 301.5 13 373.5 14 434.0 15 498.5 16 600.5 17 751.5 18 917.5 19 1181.5 20 1529.0 21 2051.5 22 2972.5 23 4378.5 24 6351.5 25 9314.0 26 13756.5 27 20189.5 28 29208.5 29 39126.0 30 48996.5 31 60263.0 32 71878.5 33 83030.5 34 93822.5 35 103142.0 36 111113.0 37 119014.5 38 125381.0 39 129495.0 40 132300.0 41 132567.5 42 130616.5 43 127859.0 44 123366.0 45 118534.0 46 113996.0 47 109518.5 48 105456.5 49 102997.0 50 109183.5 51 104935.5 52 88033.5 53 79130.5 54 74230.5 55 69375.0 56 63818.5 57 57806.5 58 52010.0 59 46853.0 60 41722.0 61 35887.0 62 30379.5 63 24762.5 64 19735.0 65 16329.0 66 12907.5 67 9849.5 68 7793.5 69 6076.5 70 4477.5 71 3299.5 72 2559.5 73 1941.0 74 1385.5 75 966.5 76 624.5 77 397.0 78 248.0 79 125.5 80 67.0 81 35.5 82 21.0 83 10.5 84 7.0 85 7.5 86 4.5 87 1.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8857382009141733 2 6.201954224793461 3 0.0 4 0.005113997473192333 5 0.15785616296769586 6 0.05252629934815017 7 0.03438085048242556 8 0.04128166634986582 9 0.04008018501580256 10-11 0.04103520864031438 12-13 0.03972590205832237 14-15 0.04215967194014282 16-17 0.03316396554151534 18-19 0.02758785986291406 20-21 0.022550880423956553 22-23 0.022812741740354955 24-25 0.019932267259972526 26-27 0.013139276640461025 28-29 0.01791439476302013 30-31 0.021888525329537063 32-33 0.001740607573707029 34-35 0.003743076463812461 36-37 0.004467045985619809 38-39 0.006577340123653994 40-41 0.0069470266879811515 42-43 0.007285906038614377 44-45 0.008225526056279233 46-47 0.01585031144552684 48-49 0.023829379792254635 50-51 0.02105673055980096 52-53 0.027187366084892972 54-55 0.023644536510091057 56-57 0.015434414060658787 58-59 0.014556408470381789 60-61 0.012091831374867414 62-63 0.017652533446621727 64-65 0.019362333806634827 66-67 0.019993881687360385 68-69 0.02737220936705655 70-71 0.02707954083696422 72-73 0.02361372929639713 74-75 0.02479980702361342 76-77 0.01836109936158211 78-79 0.014833673393627159 80-81 0.013170083854154954 82-83 0.008194718842585304 84-85 0.01087494643395719 86-87 0.00871844147538211 88-89 0.007316713252308307 90-91 0.007547767355012781 92-93 0.004158973848680512 94-95 0.0015711678983904155 96-97 0.0010474452655936104 98-99 7.393731286543132E-4 100 0.10822574170677508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3245993.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.80502930537658 #Duplication Level Percentage of deduplicated Percentage of total 1 86.87101578346478 68.45872944603772 2 9.244988874116014 14.571032383051858 3 2.0754440991720995 4.906662991707846 4 0.7273306421239029 2.2926925026909015 5 0.3374590350218153 1.3296734572129127 6 0.18656928637860046 0.8821558848329284 7 0.12438852829936294 0.6861709132518773 8 0.08082508309380919 0.5095538433453705 9 0.05955006902725636 0.4223560441347122 >10 0.2760196039901058 3.6859183860818274 >50 0.011575167862001816 0.6155164898595821 >100 0.004008923645746567 0.6223086856578256 >500 3.5353020389206053E-4 0.1753939136231811 >1k 4.32092467276217E-4 0.6358880592734621 >5k 3.9281133388747E-5 0.20594699923796828 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6653 0.2049603927057144 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4752 0.146395879473554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.5403606846964857E-4 0.0 0.0 0.0 6 0.0 1.5403606846964857E-4 3.080721369392972E-5 3.080721369392972E-5 0.0 7 0.0 1.5403606846964857E-4 3.080721369392972E-5 3.080721369392972E-5 3.080721369392972E-5 8 0.0 1.5403606846964857E-4 3.080721369392972E-5 3.080721369392972E-5 3.080721369392972E-5 9 0.0 1.5403606846964857E-4 3.080721369392972E-5 6.161442738785943E-5 3.080721369392972E-5 10-11 0.0 1.5403606846964857E-4 3.080721369392972E-5 6.161442738785943E-5 3.080721369392972E-5 12-13 0.0 1.5403606846964857E-4 3.080721369392972E-5 6.161442738785943E-5 3.080721369392972E-5 14-15 1.540360684696486E-5 2.0024688901054314E-4 4.6210820540894575E-5 7.70180342348243E-5 3.080721369392972E-5 16-17 4.6210820540894575E-5 2.4645770955143774E-4 6.161442738785943E-5 1.3863246162268373E-4 3.080721369392972E-5 18-19 6.161442738785943E-5 2.4645770955143774E-4 6.161442738785943E-5 1.5403606846964857E-4 3.080721369392972E-5 20-21 6.161442738785943E-5 2.4645770955143774E-4 6.161442738785943E-5 1.848432821635783E-4 3.080721369392972E-5 22-23 6.161442738785943E-5 2.4645770955143774E-4 6.161442738785943E-5 2.7726492324536747E-4 3.080721369392972E-5 24-25 6.161442738785943E-5 2.4645770955143774E-4 9.242164108178915E-5 4.004937780210863E-4 3.080721369392972E-5 26-27 6.161442738785943E-5 2.4645770955143774E-4 1.2322885477571887E-4 5.237226327968052E-4 3.080721369392972E-5 28-29 6.161442738785943E-5 2.4645770955143774E-4 1.2322885477571887E-4 0.0011860777272162941 3.080721369392972E-5 30-31 6.161442738785943E-5 2.4645770955143774E-4 1.2322885477571887E-4 0.003573636788495847 3.080721369392972E-5 32-33 9.242164108178915E-5 2.4645770955143774E-4 1.2322885477571887E-4 0.009011110005474443 3.080721369392972E-5 34-35 9.242164108178915E-5 2.4645770955143774E-4 1.2322885477571887E-4 0.018653767891674444 3.080721369392972E-5 36-37 9.242164108178915E-5 2.4645770955143774E-4 1.2322885477571887E-4 0.03328719439629106 3.080721369392972E-5 38-39 9.242164108178915E-5 2.618613163984026E-4 1.2322885477571887E-4 0.05620776138457477 3.080721369392972E-5 40-41 9.242164108178915E-5 2.7726492324536747E-4 1.2322885477571887E-4 0.08357997075163132 3.080721369392972E-5 42-43 9.242164108178915E-5 2.7726492324536747E-4 1.2322885477571887E-4 0.11104460175976966 3.080721369392972E-5 44-45 9.242164108178915E-5 2.7726492324536747E-4 1.2322885477571887E-4 0.1439467059848866 3.080721369392972E-5 46-47 9.242164108178915E-5 2.7726492324536747E-4 1.2322885477571887E-4 0.1873848772933275 3.080721369392972E-5 48-49 9.242164108178915E-5 2.7726492324536747E-4 1.3863246162268373E-4 0.2321785660043013 3.080721369392972E-5 50-51 9.242164108178915E-5 2.7726492324536747E-4 1.6943967531661344E-4 0.2810542105297208 3.080721369392972E-5 52-53 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 0.33019171637153866 3.080721369392972E-5 54-55 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 0.3881862961503614 3.080721369392972E-5 56-57 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 0.4596744355271253 3.080721369392972E-5 58-59 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 0.5369697346851949 4.6210820540894575E-5 60-61 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 0.6200105791971825 6.161442738785943E-5 62-63 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 0.7064710244291963 6.161442738785943E-5 64-65 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 0.8049154757881487 7.70180342348243E-5 66-67 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 0.9185786907119023 9.242164108178915E-5 68-69 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 1.049309102022093 9.242164108178915E-5 70-71 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 1.198862720899275 9.242164108178915E-5 72-73 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 1.3523442595224329 1.2322885477571887E-4 74-75 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 1.5117099759611312 1.2322885477571887E-4 76-77 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 1.6907923091639447 1.2322885477571887E-4 78-79 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 1.8937656365864006 1.2322885477571887E-4 80-81 9.242164108178915E-5 2.7726492324536747E-4 1.848432821635783E-4 2.1176262548933407 1.2322885477571887E-4 82-83 9.242164108178915E-5 2.926685300923323E-4 1.848432821635783E-4 2.3615115621013354 1.2322885477571887E-4 84-85 9.242164108178915E-5 3.0807213693929714E-4 2.0024688901054314E-4 2.6122052635356887 1.2322885477571887E-4 86-87 9.242164108178915E-5 3.0807213693929714E-4 2.15650495857508E-4 2.884618050624262 1.2322885477571887E-4 88 9.242164108178915E-5 3.0807213693929714E-4 2.15650495857508E-4 3.107153958742363 1.2322885477571887E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGTAC 690 0.0 18.394184 3 GTACCGA 160 0.0012885461 17.606026 6 ACCGCGT 165 0.0015846549 17.070667 8 CATGGGG 5595 0.0 16.804642 4 GTATCAA 12240 0.0 16.388115 1 GAGTACT 4400 0.0 16.376667 12-13 GTACCGG 245 4.6146757E-5 16.374737 1 TACACCG 295 1.3721965E-6 15.936173 5 GTACATG 15435 0.0 15.919885 1 CAACGCA 13395 0.0 15.512637 5 ACATGGG 15055 0.0 15.3933115 3 TACATGG 15740 0.0 15.312307 2 TCAACGC 13440 0.0 15.285566 4 ATCAACG 13485 0.0 15.268242 3 AACGCAG 13515 0.0 15.250304 6 CCTAGTA 1005 0.0 14.957376 2 TATCAAC 13600 0.0 14.88479 2 GTATATA 2140 0.0 14.528753 1 GTATAGA 1360 0.0 14.380572 1 CGCAGAG 14600 0.0 14.0511465 8 >>END_MODULE