FastQCFastQC Report
Wed 25 May 2016
SRR1295121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317118
Sequences flagged as poor quality0
Sequence length100
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28120.8867361676095333No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22550.7110917702558669No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16200.510850850472064No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10550.3326837328691528No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10270.32385421199679615No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9580.3020957498470601No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG8030.25321804501794287No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7350.2317749228993624No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6320.19929489969033612No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6040.19046537881797942No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5790.18258187803908954No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5560.17532905732251086No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC4410.13906495373961744No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC4080.1286587327114828No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA3810.12014455187028172No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT3690.11636047149641458No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3610.11383775124716983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA956.3949465E-638.4205471
ATAAGTG700.004076331431.4552763
TTAGACT700.004080450631.4487864
GTACATA1953.8287908E-828.0765551
TAGAGTT750.00937843926.5236645
TGTACTG1251.6481326E-423.8712985
CTTAGAC1300.003216591221.1718183
AAGAGTG1200.003644377220.7216155
AGACTCC1250.00457144519.9261766
TACATGA2551.2289383E-519.0847172
TACATAA2000.001008593618.249762
TGTCGCC700.00875385317.80951910-11
GTGCGTT900.001890783916.62221710-11
ATGGGAG3403.3195338E-614.6270225
GCTCTGT2050.004521565514.5801296
TGCCCAG2851.7889535E-413.9598245
TGTGCGT1150.0096377313.00869110-11
CATGGCA3150.002804517712.2300834
CCAGCAC3200.003150822112.04147153
ATGTACC3050.001077855611.28217794
Produced by FastQC (version 0.11.5)