##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295121_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 317118 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.339211271514074 33.0 27.0 33.0 6.0 33.0 2 22.334266739825555 27.0 6.0 33.0 2.0 33.0 3 24.81865740828335 27.0 15.0 33.0 15.0 33.0 4 25.105323570405968 33.0 15.0 37.0 2.0 37.0 5 27.056288195561272 33.0 15.0 37.0 2.0 37.0 6 27.5862581121223 33.0 15.0 37.0 6.0 37.0 7 27.53674342043025 33.0 15.0 37.0 6.0 37.0 8 28.2712712618016 33.0 22.0 37.0 6.0 37.0 9 29.09260275354915 33.0 27.0 37.0 15.0 37.0 10-11 28.699048934466035 35.0 24.5 37.0 10.5 37.0 12-13 27.624590846309577 33.0 15.0 37.0 4.0 37.0 14-15 29.356477714919997 33.0 22.0 37.0 6.0 40.0 16-17 29.719032032240364 35.0 27.0 38.5 6.0 40.0 18-19 29.81355678327941 35.0 27.0 40.0 6.0 40.0 20-21 29.311648976090922 33.0 24.5 38.5 6.0 40.0 22-23 30.130618571005115 35.0 27.0 40.0 15.0 40.0 24-25 30.26134435762082 33.0 27.0 40.0 15.0 40.0 26-27 28.80835840286581 33.0 22.0 37.0 4.0 40.0 28-29 29.510992753486086 33.0 22.0 37.0 6.0 40.0 30-31 30.192314848100708 33.0 27.0 38.5 15.0 40.0 32-33 28.89118088534867 33.0 22.0 37.0 6.0 40.0 34-35 28.610695387836707 33.0 22.0 37.0 6.0 40.0 36-37 28.896445487168815 33.0 22.0 37.0 6.0 40.0 38-39 28.94686678145044 33.0 22.0 37.0 6.0 40.0 40-41 28.056606688992744 33.0 22.0 37.0 6.0 40.0 42-43 28.634864624524624 33.0 22.0 37.0 6.0 40.0 44-45 28.38759546919443 33.0 22.0 37.0 6.0 40.0 46-47 27.949643350424765 33.0 22.0 37.0 6.0 40.0 48-49 27.787883374642877 33.0 22.0 37.0 6.0 40.0 50-51 27.75447782844241 33.0 22.0 37.0 6.0 40.0 52-53 28.0331012430704 33.0 22.0 37.0 6.0 40.0 54-55 27.94484072175026 33.0 22.0 37.0 6.0 40.0 56-57 27.86599625376043 33.0 22.0 37.0 6.0 40.0 58-59 27.654067255721845 33.0 22.0 37.0 6.0 40.0 60-61 27.599757818856073 33.0 22.0 37.0 6.0 40.0 62-63 27.408243934434502 33.0 22.0 37.0 6.0 40.0 64-65 27.00367686476327 33.0 22.0 37.0 6.0 37.0 66-67 27.219981205734143 33.0 22.0 37.0 6.0 37.0 68-69 26.727385389665677 30.0 22.0 37.0 6.0 37.0 70-71 26.760786205765676 27.0 22.0 37.0 6.0 37.0 72-73 26.71922123625906 27.0 22.0 35.0 6.0 37.0 74-75 26.79341286208919 27.0 22.0 33.0 6.0 37.0 76-77 26.49724708152801 27.0 22.0 33.0 6.0 37.0 78-79 25.99683083268689 27.0 22.0 33.0 6.0 37.0 80-81 25.92424586431549 27.0 22.0 33.0 6.0 37.0 82-83 26.08739018283415 27.0 22.0 33.0 6.0 37.0 84-85 25.936008047477593 27.0 22.0 33.0 6.0 37.0 86-87 25.66198544390416 27.0 22.0 33.0 6.0 37.0 88-89 25.537489199603932 27.0 22.0 33.0 6.0 37.0 90-91 25.71081111762814 27.0 22.0 33.0 6.0 37.0 92-93 25.563227252946852 27.0 22.0 33.0 6.0 37.0 94-95 25.457969903947426 27.0 22.0 33.0 6.0 37.0 96-97 25.56223866194918 27.0 22.0 33.0 6.0 37.0 98-99 25.560892160016145 27.0 22.0 33.0 6.0 37.0 100 25.145970900421926 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.358908662758626 1101 2 11.25018551218226 1101 3 8.510927841754082 1101 4 12.621209426878892 1101 5 10.504371652016502 1101 6 9.992704166546126 1101 7 9.288155934281157 1101 8 8.981862748296088 1101 9 7.405679709376933 1101 10-11 7.821155189167563 1101 12-13 8.980847406649627 1101 14-15 8.061632790554345 1101 16-17 8.10531895885293 1101 18-19 8.138325347632549 1101 20-21 7.550224403263282 1101 22-23 7.269375748764439 1101 24-25 7.248057518198035 1101 26-27 8.695343954588921 1101 28-29 8.245180909811282 1101 30-31 5.262439695731622 1101 32-33 6.881172626363977 1101 34-35 8.602152114765072 1101 36-37 8.263737698880853 1101 38-39 8.356659388279216 1101 40-41 9.36968597748255 1101 42-43 8.771366163575685 1101 44-45 8.330924085268226 1101 46-47 7.384662188033548 1101 48-49 5.360340483463158 1101 50-51 6.926635337542798 1101 52-53 6.0523520463968765 1101 54-55 4.123775518251403 1101 56-57 6.2267934029597285 1101 58-59 6.3489331515576275 1101 60-61 7.0099678812433375 1101 62-63 6.530376226300131 1101 64-65 6.870589370098646 1101 66-67 6.004827455816006 1101 68-69 6.607484320582024 1101 70-71 6.270305953998982 1101 72-73 5.413029417643298 1101 74-75 4.733394699484684 1101 76-77 4.976680524497539 1101 78-79 5.141795952208721 1101 80-81 4.801767497678551 1101 82-83 4.475933703107881 1101 84-85 4.187687865294862 1101 86-87 3.7789626082791052 1101 88-89 3.9322621624432372 1101 90-91 3.785359370682592 1101 92-93 3.5890454888356302 1101 94-95 3.7972440515524006 1101 96-97 3.8159206321141212 1101 98-99 3.517466433885968 1101 100 3.23568620286445 1102 1 6.918801692593963 1102 2 10.299491192104828 1102 3 7.848926195749669 1102 4 12.47109503158429 1102 5 10.430436272781513 1102 6 9.479436525825825 1102 7 7.139693580092398 1102 8 7.904005532136267 1102 9 6.644102845196443 1102 10-11 7.155725708785802 1102 12-13 8.547558878978432 1102 14-15 6.058697952609318 1102 16-17 7.445318475917656 1102 18-19 7.653755129848857 1102 20-21 7.077064537599778 1102 22-23 6.4876131959685495 1102 24-25 6.836914591489506 1102 26-27 8.168015854395946 1102 28-29 7.480328134643813 1102 30-31 4.2819019875412465 1102 32-33 4.072863724860639 1102 34-35 4.7515260092960325 1102 36-37 4.687909817014869 1102 38-39 4.396133310780179 1102 40-41 6.123771308122169 1102 42-43 6.788815218934154 1102 44-45 6.377722526192162 1102 46-47 5.358576640073043 1102 48-49 3.509378335528009 1102 50-51 4.652298920801847 1102 52-53 5.252498536765337 1102 54-55 5.061445677479188 1102 56-57 6.274688509416773 1102 58-59 5.7604402919569715 1102 60-61 5.854392292816883 1102 62-63 4.1099257281725095 1102 64-65 5.931726964396791 1102 66-67 3.7130289042194065 1102 68-69 5.854076105450456 1102 70-71 5.228885198647962 1102 72-73 5.011640777488438 1102 74-75 3.992203740838029 1102 76-77 3.148358986187688 1102 78-79 4.22170161885057 1102 80-81 4.309895902120751 1102 82-83 3.907867999762953 1102 84-85 2.928644399107373 1102 86-87 2.7415622891393774 1102 88-89 3.3482062707334244 1102 90-91 2.9768321421877992 1102 92-93 3.1491528212017528 1102 94-95 2.9549224206799565 1102 96-97 3.000704749982525 1102 98-99 3.0433658505403933 1102 100 3.1326821441958614 1103 1 6.463586033074144 1103 2 10.687359278107778 1103 3 7.843867552814412 1103 4 11.54701882085098 1103 5 10.088380910640069 1103 6 8.829722968221311 1103 7 7.983868916950662 1103 8 7.860580664321155 1103 9 7.028200526568156 1103 10-11 7.255998606619677 1103 12-13 8.63443169052844 1103 14-15 8.349665234968281 1103 16-17 7.73845188483298 1103 18-19 6.886095529764585 1103 20-21 7.900537764516333 1103 22-23 6.535702409481004 1103 24-25 5.182422311889621 1103 26-27 5.465632011552994 1103 28-29 7.003765242729415 1103 30-31 5.767760461921959 1103 32-33 7.889108169082128 1103 34-35 7.0093000860084125 1103 36-37 4.198239802667672 1103 38-39 6.260415800170268 1103 40-41 6.299301803451808 1103 42-43 7.281040640571341 1103 44-45 5.633787981540255 1103 46-47 6.091424710223841 1103 48-49 5.625746185755258 1103 50-51 6.150536121191131 1103 52-53 6.0707957345460315 1103 54-55 4.569220255842001 1103 56-57 5.987041149602984 1103 58-59 5.312600900358614 1103 60-61 4.7612679136092915 1103 62-63 2.8236548971384074 1103 64-65 5.097230470933294 1103 66-67 4.198338573029787 1103 68-69 5.291859412530751 1103 70-71 3.6284126439429834 1103 72-73 4.4761881383055275 1103 74-75 2.8672426187463493 1103 76-77 3.2616176070503258 1103 78-79 2.856185069402887 1103 80-81 4.229360942102289 1103 82-83 3.23143052045603 1103 84-85 3.0027321478005717 1103 86-87 2.657908066747705 1103 88-89 3.1752843576393452 1103 90-91 2.876450887861065 1103 92-93 1.7514764659931146 1103 94-95 2.3514570195101108 1103 96-97 2.734400609359877 1103 98-99 2.3015482707027246 1103 100 2.3746361729504635 1104 1 5.998833593536602 1104 2 9.516884194452178 1104 3 7.147189732067822 1104 4 11.43904198393431 1104 5 9.337824876416413 1104 6 7.954719461699177 1104 7 3.776320877597467 1104 8 6.2467268963126585 1104 9 6.380940623147794 1104 10-11 6.867218475575939 1104 12-13 7.15370453802478 1104 14-15 6.912171496614942 1104 16-17 6.817102174371524 1104 18-19 6.758429070149699 1104 20-21 7.146849905097813 1104 22-23 6.245483602500023 1104 24-25 5.875878139729405 1104 26-27 8.126332915233839 1104 28-29 7.121744684491091 1104 30-31 6.371633666081699 1104 32-33 7.332025996240645 1104 34-35 8.105975903028071 1104 36-37 6.136921751091741 1104 38-39 7.121496410104751 1104 40-41 7.080552930548205 1104 42-43 6.986438680926398 1104 44-45 6.244451816272861 1104 46-47 6.620315446994237 1104 48-49 5.113939224807897 1104 50-51 5.303543715316202 1104 52-53 5.643105230207965 1104 54-55 4.556515625229061 1104 56-57 5.412927297838703 1104 58-59 3.304279422409632 1104 60-61 3.5617638360249835 1104 62-63 3.45343228307625 1104 64-65 2.298794379803791 1104 66-67 2.8541063010038243 1104 68-69 3.7823246779243824 1104 70-71 1.9603530531040185 1104 72-73 2.3962835157074913 1104 74-75 2.6692693885381615 1104 76-77 3.448421061475557 1104 78-79 3.761550048263569 1104 80-81 2.6549625611136527 1104 82-83 2.9858166787103855 1104 84-85 2.9227523854425854 1104 86-87 0.24909868129075718 1104 88-89 0.08418691639863596 1104 90-91 1.9451326359124401 1104 92-93 1.7333347223499374 1104 94-95 2.0193543084675696 1104 96-97 2.6975841307095862 1104 98-99 2.2586715199463185 1104 100 0.5736242908497573 1105 1 5.220955396384102 1105 2 -2.401494442429108 1105 3 0.6464926504890158 1105 4 7.864687290994695 1105 5 8.55995883766354 1105 6 8.071338778153951 1105 7 5.324500562532805 1105 8 5.8180014613459115 1105 9 4.457579068817985 1105 10-11 4.648964057010872 1105 12-13 4.741189289365241 1105 14-15 5.297375940104729 1105 16-17 5.842136319210486 1105 18-19 5.622106053893912 1105 20-21 6.872823894228208 1105 22-23 1.5780452151901443 1105 24-25 3.6791572601097187 1105 26-27 6.9403681664871755 1105 28-29 5.7364632378036475 1105 30-31 4.887032279213578 1105 32-33 6.905187139616551 1105 34-35 6.098853107244604 1105 36-37 6.012666959669083 1105 38-39 3.7476671626691562 1105 40-41 5.037966109442845 1105 42-43 5.5447017319552 1105 44-45 5.603706182151527 1105 46-47 5.08833176356654 1105 48-49 4.644929972265775 1105 50-51 5.673782182060954 1105 52-53 5.311104930300772 1105 54-55 4.82818999897016 1105 56-57 4.934386873186334 1105 58-59 1.4655281711076675 1105 60-61 3.9391919697511675 1105 62-63 -0.3282981003591452 1105 64-65 -0.7314547052766329 1105 66-67 0.7578948583286476 1105 68-69 2.1499389567489082 1105 70-71 2.4702510036031207 1105 72-73 2.438523822423388 1105 74-75 2.3867132471278687 1105 76-77 1.7861712712079516 1105 78-79 3.228974656053861 1105 80-81 1.6261127051906854 1105 82-83 0.3683797098804682 1105 84-85 0.8096539388807784 1105 86-87 -1.0079369792953585 1105 88-89 1.363969929493237 1105 90-91 1.2317648807988206 1105 92-93 1.398218832537463 1105 94-95 0.9227223878051802 1105 96-97 1.9550443220827987 1105 98-99 1.8458178624970678 1105 100 1.9315169159698193 1106 1 5.964770208015224 1106 2 10.012182521163066 1106 3 6.524443606594886 1106 4 11.422653407259041 1106 5 9.921659510493257 1106 6 8.964494826197587 1106 7 5.946154973435615 1106 8 7.37766137366706 1106 9 6.507976827615174 1106 10-11 5.291489082994133 1106 12-13 7.502560385038347 1106 14-15 7.183093546987752 1106 16-17 7.528378670754247 1106 18-19 6.796966564806883 1106 20-21 7.771367073329063 1106 22-23 5.889306126206272 1106 24-25 4.5942447436015605 1106 26-27 7.391694484991163 1106 28-29 6.260903132463987 1106 30-31 5.063094271758537 1106 32-33 6.9985158884346035 1106 34-35 6.5808943756966265 1106 36-37 5.156941460126234 1106 38-39 6.457863932148793 1106 40-41 5.990492932764479 1106 42-43 5.667146385654981 1106 44-45 4.068164792574525 1106 46-47 5.580578788190071 1106 48-49 5.018431539075969 1106 50-51 5.855143392372987 1106 52-53 5.320694316626934 1106 54-55 5.232838267664434 1106 56-57 4.383456750576599 1106 58-59 4.161948928021559 1106 60-61 4.483016744888506 1106 62-63 4.485235857614395 1106 64-65 4.600584023458115 1106 66-67 1.8984496497983265 1106 68-69 2.471855935344955 1106 70-71 2.599386835497281 1106 72-73 2.07514498256624 1106 74-75 2.1157937913306704 1106 76-77 3.068206874136834 1106 78-79 3.5372865645927796 1106 80-81 3.224434928888982 1106 82-83 1.3795818166736247 1106 84-85 2.1198862644022043 1106 86-87 2.859008209698679 1106 88-89 2.7857632433944737 1106 90-91 2.3892507951361104 1106 92-93 2.3141971707027693 1106 94-95 1.2287944610740702 1106 96-97 1.0730649836596093 1106 98-99 1.4289330406289302 1106 100 1.919969016902094 1107 1 6.134344903833014 1107 2 10.585390876139378 1107 3 6.301831810778669 1107 4 11.397213071045229 1107 5 9.531271353530514 1107 6 8.553136591634932 1107 7 3.787598520680266 1107 8 6.553717157457648 1107 9 5.553737952105582 1107 10-11 4.927961828582902 1107 12-13 6.253325759422506 1107 14-15 6.293915429218476 1107 16-17 7.15190694828804 1107 18-19 6.518872012541063 1107 20-21 5.929701193679765 1107 22-23 6.071612795391388 1107 24-25 4.663100892568206 1107 26-27 7.578310074231055 1107 28-29 6.618567107531284 1107 30-31 5.184556628718127 1107 32-33 5.060721740695705 1107 34-35 7.045728472436803 1107 36-37 7.1053623097901735 1107 38-39 6.496585186339654 1107 40-41 7.809907864057866 1107 42-43 6.264891974422671 1107 44-45 4.550635948388223 1107 46-47 3.766651335450465 1107 48-49 4.321938881947954 1107 50-51 5.2190287396837505 1107 52-53 3.754944918695216 1107 54-55 5.390212226833974 1107 56-57 5.284593597155435 1107 58-59 3.275395513153228 1107 60-61 2.519957372298748 1107 62-63 0.3831990566825674 1107 64-65 1.9652957389985595 1107 66-67 4.2448809195721395 1107 68-69 4.75875917943052 1107 70-71 4.1217951873255245 1107 72-73 2.0976851598025483 1107 74-75 1.8931426446463746 1107 76-77 2.8174710129037344 1107 78-79 3.7161047793225954 1107 80-81 1.8266802894216347 1107 82-83 1.4955330845585948 1107 84-85 2.009038804107231 1107 86-87 2.0689155777552166 1107 88-89 2.3931812755362643 1107 90-91 1.5200924315026079 1107 92-93 2.1688035833202335 1107 94-95 1.954733372786464 1107 96-97 2.139036713995978 1107 98-99 1.267606286760742 1107 100 -0.5198989215846304 1108 1 4.320578087341403 1108 2 -2.64820406862723 1108 3 0.33932686189769967 1108 4 6.769729897240527 1108 5 6.828553544545674 1108 6 6.662893067876421 1108 7 4.46467530402408 1108 8 4.895997291290914 1108 9 4.9527846617789955 1108 10-11 3.1046524051372373 1108 12-13 2.323196195777953 1108 14-15 3.876555919215914 1108 16-17 4.722819560753795 1108 18-19 4.051996522504812 1108 20-21 4.722537154771853 1108 22-23 4.225707363654898 1108 24-25 3.2398745326933387 1108 26-27 4.973869278373368 1108 28-29 6.212921889896968 1108 30-31 4.633959657821428 1108 32-33 4.474941137203928 1108 34-35 2.690930580854168 1108 36-37 0.44905724135976754 1108 38-39 1.3984867210749776 1108 40-41 5.338133952229459 1108 42-43 2.7538336513752455 1108 44-45 3.8758841687318117 1108 46-47 4.484143980054828 1108 48-49 4.411135183678315 1108 50-51 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86-87 -4.83311452322776 2212 88-89 -3.6100779240580145 2212 90-91 -4.3352871123586105 2212 92-93 -4.416487507206462 2212 94-95 -3.4538188564463965 2212 96-97 -4.985864465879018 2212 98-99 -3.5895072706145967 2212 100 -3.513783261018709 2213 1 -21.21893144944334 2213 2 -16.96019316944467 2213 3 -19.963125454178595 2213 4 -18.901932228100073 2213 5 -21.21231839005923 2213 6 -21.773074234575894 2213 7 -11.124223753737427 2213 8 -10.59403952465018 2213 9 -4.012593793615476 2213 10-11 -17.676521544984443 2213 12-13 -16.402847442552225 2213 14-15 -23.716768331465286 2213 16-17 -17.986682904042265 2213 18-19 -18.801010112684573 2213 20-21 -17.61542070449557 2213 22-23 -19.795560602316357 2213 24-25 -7.609658508053794 2213 26-27 -23.4266756807547 2213 28-29 -24.3109200719559 2213 30-31 -19.625462727102757 2213 32-33 -23.53187085189689 2213 34-35 -18.00084511230472 2213 36-37 -23.90176019733233 2213 38-39 -11.729812673645991 2213 40-41 -17.252579902628373 2213 42-43 -23.270008310477614 2213 44-45 -17.08225589824408 2213 46-47 -17.012621625120204 2213 48-49 -6.5491056799363 2213 50-51 -23.2788345081795 2213 52-53 -17.609754631118836 2213 54-55 -17.59156285417775 2213 56-57 -17.505629538487547 2213 58-59 -17.748625278043136 2213 60-61 -18.860194212051308 2213 62-63 -17.359140170389487 2213 64-65 -17.21208731052189 2213 66-67 -8.626016842547 2213 68-69 -22.22842030774897 2213 70-71 -16.80578903224343 2213 72-73 -8.797804281488421 2213 74-75 -3.635439813325398 2213 76-77 -3.0533506308186737 2213 78-79 -4.5251633531669135 2213 80-81 -3.369835662819508 2213 82-83 -3.3900609651575913 2213 84-85 -2.9778742686837063 2213 86-87 -2.971318431944443 2213 88-89 -2.3380664137266933 2213 90-91 -2.3612552990817655 2213 92-93 -3.198750406371161 2213 94-95 -3.5792534913530716 2213 96-97 -3.1213806322992284 2213 98-99 -3.648467743817104 2213 100 -3.169852163140927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81.0 3 90.0 4 618.0 5 1722.0 6 2498.0 7 2975.0 8 2756.0 9 2796.0 10 3338.0 11 3982.0 12 5158.0 13 6174.0 14 5352.0 15 5753.0 16 6374.0 17 6572.0 18 6411.0 19 6632.0 20 6444.0 21 6573.0 22 6969.0 23 7694.0 24 8392.0 25 8834.0 26 9842.0 27 10298.0 28 10955.0 29 11874.0 30 13023.0 31 14631.0 32 16588.0 33 19780.0 34 23153.0 35 25248.0 36 25129.0 37 17886.0 38 4518.0 39 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.17572677206346 21.41169137189358 12.629462695564019 23.783119160478943 2 14.627359775389193 24.797703605925367 35.307058897479415 25.267877721206027 3 19.50477742938262 29.29303475382009 28.016583759097664 23.185604057699628 4 13.788419776984325 21.664728225065268 32.133939536888676 32.41291246106172 5 12.75153834851075 40.19739600692391 33.062039257005445 13.989026387559894 6 30.78161587382702 35.111286895832386 18.01556161468378 16.09153561565682 7 24.245547847539335 34.89910286937489 22.241692694106288 18.61365658897948 8 24.91201504560006 36.400879503827085 20.805589551052016 17.881515899520835 9 25.051204298501112 20.27091728843315 20.151218928300683 34.52665948476506 10-11 22.38248370088239 30.83125499817016 25.470336283665638 21.31592501728181 12-13 24.461493839154045 28.65579636709277 25.188005395545183 21.69470439820801 14-15 23.656036562747786 29.32234363674107 23.307730788733068 23.71388901177808 16-17 21.010904601879158 32.20732460050751 26.298779233248748 20.482991564364582 18-19 22.130495291564095 31.28629525160757 26.961509679298118 19.621699777530214 20-21 22.223251553002815 30.57335440030331 26.191150157058722 21.012243889635155 22-23 21.262085678732387 30.833993478381018 24.43882088324895 23.46509995963764 24-25 22.047979713723265 31.341630242567813 23.565279770444764 23.045110273264154 26-27 22.777643349850436 29.128135531405285 24.580672537075806 23.513548581668474 28-29 22.603932821335135 28.791290404253523 26.318001519115537 22.286775255295804 30-31 23.820435663426576 30.223242861573592 25.031510040319656 20.924811434680183 32-33 20.744255034533904 30.063730909317744 26.25755050128744 22.934463554860912 34-35 19.921465790511952 30.307436431166074 26.491265662626017 23.27983211569596 36-37 23.08295218577381 29.912222961143307 24.87411951520745 22.130705337875437 38-39 23.79396786380197 28.693922637071463 24.78592961106693 22.72617988805964 40-41 21.943032445780396 30.03435799726237 24.924508704713823 23.098100852243416 42-43 23.633796250941653 29.141175794960496 25.150411626202196 22.074616327895654 44-45 24.458728525298202 28.402790802989518 24.85784917565195 22.280631496060334 46-47 22.152844926890385 29.339196677064855 27.30356256989347 21.2043958261513 48-49 23.02923770827407 28.92808224246201 26.778129845614508 21.26455020364941 50-51 21.63744768422852 29.278892775727172 26.64419182816226 22.439467711882045 52-53 21.00056220649514 29.456643957933725 24.88755870097229 24.65523513459885 54-55 21.832640700847623 30.357258672041425 23.96475140896248 23.845349218148467 56-57 22.951999893834437 28.196412930965842 24.440971945749396 24.41061522945033 58-59 22.44827603189378 27.552626870033016 26.30760719497921 23.691489903094 60-61 23.4397471928644 28.762914143213397 25.28533164443389 22.512007019488316 62-63 20.93630737241431 29.093187499388918 26.188675924688674 23.7818292035081 64-65 19.893490399402726 29.290636789680786 26.638325908783152 24.177546902133336 66-67 22.553226631775992 28.53510982142279 25.05732347930849 23.85434006749273 68-69 23.89494874147287 27.563051235216445 24.676189635737273 23.865810387573408 70-71 21.862012445377363 28.733532226141772 24.966068368593074 24.43838695988779 72-73 23.314387410715 28.05092150936741 25.16045245749192 23.474238622425666 74-75 23.812384766990906 27.42815817915951 24.838514845190414 23.920942208659167 76-77 21.965340044592875 28.37181781354564 26.698787571410797 22.96405457045068 78-79 23.01901584144737 28.10800650943563 26.401448397621774 22.471529251495227 80-81 21.709890308194016 28.209065363942205 26.35310191928436 23.72794240857942 82-83 20.961955130803577 28.66583051569532 25.100425546796636 25.27178880670447 84-85 21.680704759105055 28.94350040580525 24.248141923197363 25.12765291189233 86-87 22.52929520577864 27.429713663499218 24.824007191648573 25.216983939073568 88-89 22.447052175610747 26.656053027982228 26.409865287211094 24.487029509195928 90-91 23.14365336920492 27.661797552112066 25.431889565257254 23.762659513425763 92-93 21.115602749388724 28.079381798742975 26.049445978560335 24.755569473307965 94-95 20.275906472812157 28.21975463703027 26.415055441374236 25.089283448783334 96-97 22.395621827411166 27.544257614213198 25.475888324873097 24.584232233502537 98-99 22.995331033969222 27.033582651155204 25.04701444416807 24.924071870707508 100 21.589682044591655 27.90635815819556 25.861703171954314 24.642256625258472 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1623.0 1 1693.0 2 1508.5 3 1063.5 4 714.0 5 458.5 6 323.5 7 311.5 8 323.0 9 340.5 10 395.0 11 432.0 12 494.5 13 593.5 14 699.5 15 800.0 16 873.5 17 957.5 18 1065.5 19 1161.0 20 1228.5 21 1348.0 22 1461.0 23 1605.5 24 1813.5 25 2077.5 26 2404.5 27 2812.0 28 3314.5 29 3790.5 30 4285.0 31 4800.5 32 5426.0 33 5953.0 34 6299.0 35 6678.5 36 7076.5 37 7504.0 38 7907.5 39 8217.0 40 8362.0 41 8443.0 42 8363.5 43 8234.0 44 8323.0 45 8397.0 46 8820.0 47 9850.0 48 11462.5 49 14638.0 50 19055.5 51 19119.0 52 14178.0 53 10292.0 54 8405.0 55 7258.0 56 6463.0 57 5615.0 58 4907.0 59 4257.5 60 3665.5 61 3157.5 62 2629.5 63 2254.5 64 1935.0 65 1642.0 66 1380.0 67 1109.5 68 894.5 69 701.0 70 531.0 71 449.5 72 369.0 73 259.0 74 193.5 75 142.0 76 98.0 77 72.0 78 53.0 79 37.0 80 23.0 81 18.0 82 10.5 83 6.5 84 5.0 85 3.0 86 2.5 87 4.0 88 4.0 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.928279063313972 2 24.748831665184568 3 6.370183969374176 4 22.45725565877686 5 12.009725086560838 6 9.90640707875302 7 8.223752672506764 8 8.7866346281195 9 5.159593589767847 10-11 6.940634085734648 12-13 9.761666004452602 14-15 7.337016504897232 16-17 8.040540114405363 18-19 6.873466659098505 20-21 8.093674909655082 22-23 5.85665272863729 24-25 5.4894392623565995 26-27 10.077952055701662 28-29 6.588399270933848 30-31 4.427374037424555 32-33 9.006426629834952 34-35 7.2862467598811795 36-37 7.061724657698395 38-39 6.220870464622002 40-41 8.311101861136864 42-43 6.65272863728959 44-45 6.690727111043839 46-47 6.240736886584805 48-49 4.112507016315694 50-51 6.835468185344257 52-53 4.64748137917116 54-55 5.188447202618584 56-57 4.951469169205154 58-59 3.9557830208313627 60-61 4.402777514994419 62-63 3.2432722204352955 64-65 4.755169999810796 66-67 2.631197219962285 68-69 5.306069034239621 70-71 2.651694321987399 72-73 1.6604229340497858 74-75 0.8003329990729002 76-77 1.1399542126274762 78-79 1.8534110331170102 80-81 2.142420171671113 82-83 0.8140502904281687 84-85 1.3118145296072756 86-87 0.3771466772620917 88-89 0.6667864958785058 90-91 0.25369105506467626 92-93 0.3074565303767052 94-95 0.577072257014739 96-97 0.6048221797564314 98-99 0.48042053746554914 100 0.41593350109422994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 317118.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.31566496143037 #Duplication Level Percentage of deduplicated Percentage of total 1 96.35265266462912 82.2039063288057 2 2.495054259232123 4.257344264824753 3 0.5258053372105586 1.345782959531638 4 0.18191635668855236 0.6208125975297838 5 0.1022260302669293 0.43607408742951903 6 0.062194656161505574 0.3183707068479823 7 0.038994430121846044 0.2328785014946128 8 0.031800648206986866 0.21704747583868828 9 0.028804718275557716 0.22117443243352855 >10 0.14919776063449816 2.44962456578397 >50 0.013976700327446811 0.8140761958231182 >100 0.012843454354877543 2.4883144528591927 >500 0.002644240602474788 1.5685308175191932 >1k 0.0018887432874819914 2.8260626132783653 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2812 0.8867361676095333 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2255 0.7110917702558669 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1620 0.510850850472064 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1055 0.3326837328691528 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1027 0.32385421199679615 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 958 0.3020957498470601 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 803 0.25321804501794287 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 735 0.2317749228993624 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 632 0.19929489969033612 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 604 0.19046537881797942 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 579 0.18258187803908954 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 556 0.17532905732251086 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 441 0.13906495373961744 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 408 0.1286587327114828 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 381 0.12014455187028172 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 369 0.11636047149641458 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 361 0.11383775124716983 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.883500778889877E-4 0.0 24-25 0.0 0.0 0.0 0.0025227202492447607 0.0 26-27 0.0 0.0 0.0 0.004257090420600533 0.0 28-29 0.0 0.0 0.0 0.007568160747734282 0.0 30-31 0.0 0.0 0.0 0.01687069166682434 0.0 32-33 0.0 0.0 0.0 0.040994204050227356 0.0 34-35 0.0 0.0 0.0 0.07331655724367586 0.0 36-37 0.0 0.0 0.0 0.11683348154314797 0.0 38-39 0.0 0.0 0.0 0.18242420802351175 0.0 40-41 0.0 0.0 0.0 0.28412136807119115 0.0 42-43 0.0 0.0 0.0 0.3577532653460226 0.0 44-45 0.0 0.0 0.0 0.438480313321855 0.0 46-47 0.0 0.0 0.0 0.5406504834162678 0.0 48-49 0.0 0.0 0.0 0.6421899734483694 0.0 50-51 0.0 0.0 0.0 0.7492479140256939 0.0 52-53 0.0 0.0 0.0 0.8816907271110439 0.0 54-55 0.0 0.0 0.0 1.0196519907416168 0.0 56-57 0.0 0.0 0.0 1.160293644637012 0.0 58-59 0.0 0.0 0.0 1.3315232815545004 0.0 60-61 0.0 0.0 0.0 1.495973107802143 0.0 62-63 0.0 0.0 0.0 1.6648376944859642 0.0 64-65 0.0 0.0 0.0 1.8778498855315686 0.0 66-67 0.0 0.0 0.0 2.1148279189449983 0.0 68-69 0.0 0.0 0.0 2.3953228766579002 0.0 70-71 0.0 0.0 0.0 2.798327436474751 0.0 72-73 0.0 0.0 0.0 3.1504045812599726 0.0 74-75 0.0 0.0 0.0 3.492706185079371 0.0 76-77 0.0 0.0 0.0 3.883727823712309 0.0 78-79 0.0 0.0 0.0 4.306914145523118 0.0 80-81 0.0 0.0 0.0 4.7591117502002405 0.0 82-83 0.0 0.0 0.0 5.332715266872269 0.0 84-85 0.0 0.0 0.0 5.876046140553358 0.0 86-87 0.0 0.0 0.0 6.407236423034959 0.0 88 0.0 0.0 0.0 6.880404139783929 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATATA 95 6.3949465E-6 38.420547 1 ATAAGTG 70 0.0040763314 31.455276 3 TTAGACT 70 0.0040804506 31.448786 4 GTACATA 195 3.8287908E-8 28.076555 1 TAGAGTT 75 0.009378439 26.523664 5 TGTACTG 125 1.6481326E-4 23.871298 5 CTTAGAC 130 0.0032165912 21.171818 3 AAGAGTG 120 0.0036443772 20.721615 5 AGACTCC 125 0.004571445 19.926176 6 TACATGA 255 1.2289383E-5 19.084717 2 TACATAA 200 0.0010085936 18.24976 2 TGTCGCC 70 0.008753853 17.809519 10-11 GTGCGTT 90 0.0018907839 16.622217 10-11 ATGGGAG 340 3.3195338E-6 14.627022 5 GCTCTGT 205 0.0045215655 14.580129 6 TGCCCAG 285 1.7889535E-4 13.959824 5 TGTGCGT 115 0.00963773 13.008691 10-11 CATGGCA 315 0.0028045177 12.230083 4 CCAGCAC 320 0.0031508221 12.0414715 3 ATGTACC 305 0.0010778556 11.282177 94 >>END_MODULE