##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295121_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 317118 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54243530799261 33.0 33.0 33.0 27.0 33.0 2 30.133013578541743 33.0 33.0 33.0 27.0 33.0 3 30.955735719826688 33.0 33.0 33.0 27.0 33.0 4 35.26667360414735 37.0 37.0 37.0 33.0 37.0 5 35.62787668943422 37.0 37.0 37.0 33.0 37.0 6 35.44726253318954 37.0 37.0 37.0 33.0 37.0 7 35.35536614131018 37.0 37.0 37.0 33.0 37.0 8 35.36871133142868 37.0 37.0 37.0 33.0 37.0 9 35.288082038862505 37.0 37.0 37.0 33.0 37.0 10-11 35.26861609873927 37.0 37.0 37.0 33.0 37.0 12-13 35.19222024609136 37.0 37.0 37.0 33.0 37.0 14-15 36.60250600722759 40.0 37.0 40.0 33.0 40.0 16-17 36.558263800856466 37.0 37.0 40.0 33.0 40.0 18-19 36.64616010444062 40.0 37.0 40.0 33.0 40.0 20-21 36.671844549978246 40.0 37.0 40.0 33.0 40.0 22-23 36.66808412010671 40.0 37.0 40.0 33.0 40.0 24-25 36.63021020566477 40.0 37.0 40.0 33.0 40.0 26-27 36.51817146929534 38.5 37.0 40.0 33.0 40.0 28-29 36.39790866491337 38.5 37.0 40.0 33.0 40.0 30-31 36.16079503528655 37.0 37.0 40.0 30.0 40.0 32-33 36.036224686078995 37.0 37.0 40.0 27.0 40.0 34-35 35.95486380464054 37.0 37.0 40.0 27.0 40.0 36-37 35.92515246690506 37.0 37.0 40.0 27.0 40.0 38-39 35.79781185552381 37.0 37.0 40.0 27.0 40.0 40-41 35.655126798226526 37.0 37.0 40.0 27.0 40.0 42-43 35.44708278937178 37.0 37.0 40.0 27.0 40.0 44-45 35.20413852256888 37.0 35.0 40.0 27.0 40.0 46-47 34.899158988136904 37.0 33.0 40.0 22.0 40.0 48-49 34.6603582893434 37.0 33.0 40.0 22.0 40.0 50-51 34.5474665581897 37.0 33.0 40.0 22.0 40.0 52-53 34.50948227473685 37.0 33.0 40.0 22.0 40.0 54-55 34.36908343266544 37.0 33.0 40.0 22.0 40.0 56-57 34.1659271942936 37.0 33.0 40.0 22.0 40.0 58-59 34.03152611961478 37.0 33.0 40.0 22.0 40.0 60-61 33.81812133023038 37.0 33.0 40.0 22.0 40.0 62-63 33.58092413549531 37.0 33.0 40.0 22.0 40.0 64-65 33.43296186277664 37.0 33.0 37.0 22.0 40.0 66-67 33.1920263119722 37.0 33.0 37.0 22.0 40.0 68-69 32.9599533927434 37.0 33.0 37.0 22.0 40.0 70-71 32.648782156799676 37.0 33.0 37.0 22.0 40.0 72-73 32.37522783317251 37.0 33.0 37.0 22.0 37.0 74-75 32.1406700344982 37.0 33.0 37.0 22.0 37.0 76-77 31.859088730377966 37.0 33.0 37.0 18.5 37.0 78-79 31.64356800938452 33.0 33.0 37.0 15.0 37.0 80-81 31.528561923321917 33.0 33.0 37.0 15.0 37.0 82-83 31.412212488726595 33.0 33.0 37.0 15.0 37.0 84-85 31.23368746018832 33.0 27.0 37.0 15.0 37.0 86-87 31.22676889990477 33.0 30.0 37.0 15.0 37.0 88-89 31.241481089058333 33.0 33.0 37.0 15.0 37.0 90-91 31.261842594870046 33.0 33.0 37.0 15.0 37.0 92-93 31.211618388107894 33.0 30.0 37.0 15.0 37.0 94-95 31.288916113244913 33.0 33.0 37.0 15.0 37.0 96-97 31.27517044128684 33.0 33.0 37.0 15.0 37.0 98-99 31.170969481391786 33.0 27.0 37.0 15.0 37.0 100 30.582149231516343 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8340830248508375 1101 2 4.065999455069029 1101 3 2.430731731385542 1101 4 1.2270551838989476 1101 5 0.7464031480548456 1101 6 0.5966943393781321 1101 7 0.801163679523448 1101 8 0.6845611102885698 1101 9 0.6257021519753749 1101 10-11 0.8492441775170221 1101 12-13 0.8253717547269517 1101 14-15 1.1754506526978687 1101 16-17 1.191715297456696 1101 18-19 1.2684692623131752 1101 20-21 1.1943879436223455 1101 22-23 1.0898005061284337 1101 24-25 1.2106961815715849 1101 26-27 1.21092121486911 1101 28-29 0.8784014196361554 1101 30-31 0.8357965812271502 1101 32-33 1.219942886510779 1101 34-35 1.409127815195255 1101 36-37 1.1978790520268277 1101 38-39 1.1356440217816584 1101 40-41 1.1227604961864515 1101 42-43 1.199797169162295 1101 44-45 1.2891737659361624 1101 46-47 1.1261411812406266 1101 48-49 1.1837026373537043 1101 50-51 1.4179620573415832 1101 52-53 1.2077649176608105 1101 54-55 1.1760352574763218 1101 56-57 1.1019581520372626 1101 58-59 1.144911817540887 1101 60-61 1.29031655258796 1101 62-63 1.0434272232321504 1101 64-65 1.1279967983666808 1101 66-67 1.1038426430570354 1101 68-69 1.1841652407290155 1101 70-71 1.2482001134910234 1101 72-73 1.0937444020195102 1101 74-75 1.0714338189635306 1101 76-77 1.1106935067902626 1101 78-79 1.0531789048116345 1101 80-81 0.9914208342529136 1101 82-83 0.6822982806857532 1101 84-85 0.9563934180032128 1101 86-87 0.8580668479888018 1101 88-89 0.9091015464096195 1101 90-91 0.9900366083583343 1101 92-93 0.9455423989975706 1101 94-95 0.8291651891100749 1101 96-97 0.9806058245797011 1101 98-99 0.9049983210669765 1101 100 0.940797636718532 1102 1 0.6314755767814759 1102 2 4.027082920371132 1102 3 2.3207693802151326 1102 4 1.180017287565029 1102 5 0.6285588906583897 1102 6 0.5500146200440241 1102 7 0.5781703259202473 1102 8 0.4690351434622002 1102 9 0.6700577452714285 1102 10-11 0.4959347628949473 1102 12-13 0.6376316704949829 1102 14-15 0.9872195842639258 1102 16-17 0.9285407223894353 1102 18-19 0.8884736489753067 1102 20-21 1.150891572679754 1102 22-23 1.0233184280579124 1102 24-25 0.8791842520640358 1102 26-27 0.791748483004838 1102 28-29 0.7067159151398315 1102 30-31 0.6970050060331161 1102 32-33 0.961555407412817 1102 34-35 0.8161999999340921 1102 36-37 0.7610248522002863 1102 38-39 0.7298284748876327 1102 40-41 0.8960887855290736 1102 42-43 0.7385527254333155 1102 44-45 0.8467084618177338 1102 46-47 0.5909663888524932 1102 48-49 0.5467935036389875 1102 50-51 0.5667604539964586 1102 52-53 0.6439822465860203 1102 54-55 0.5630183406558871 1102 56-57 0.48433926443843944 1102 58-59 0.5196615463928538 1102 60-61 0.685404899560865 1102 62-63 0.9003113650461003 1102 64-65 0.8816223343745335 1102 66-67 0.7629064327709187 1102 68-69 0.5499384100521638 1102 70-71 0.5341702399194617 1102 72-73 0.5793549428869014 1102 74-75 0.5780812218784455 1102 76-77 0.6022290598819566 1102 78-79 0.49780433819573844 1102 80-81 0.491312173814773 1102 82-83 0.6564018823768301 1102 84-85 0.6378925698481233 1102 86-87 0.664779044720536 1102 88-89 0.7451500492096379 1102 90-91 0.7147544433594781 1102 92-93 0.7615923506034328 1102 94-95 0.7592361604740212 1102 96-97 0.8605219548192942 1102 98-99 0.6455735372308204 1102 100 0.9105598514597304 1103 1 0.6252049464075462 1103 2 4.030881417426446 1103 3 2.2932568640369055 1103 4 0.9362834030850848 1103 5 0.36705654795088094 1103 6 0.517417375108181 1103 7 0.4963431143943282 1103 8 0.4905439072952049 1103 9 0.6655770036314337 1103 10-11 0.5620512862568177 1103 12-13 0.4133708524034674 1103 14-15 0.8236494384843098 1103 16-17 0.6833119196283519 1103 18-19 0.8156470494629104 1103 20-21 0.7297580433683208 1103 22-23 0.642831626480735 1103 24-25 0.9447108345203148 1103 26-27 0.9304427872658536 1103 28-29 0.9788672931735647 1103 30-31 0.6964150651275389 1103 32-33 0.7163757663938668 1103 34-35 0.7775317814954263 1103 36-37 0.7127369851175871 1103 38-39 0.7350065106252117 1103 40-41 0.7365699686214455 1103 42-43 0.4615082491062026 1103 44-45 0.7214112339380065 1103 46-47 0.7736965711522572 1103 48-49 0.4132350964794327 1103 50-51 0.268187147819134 1103 52-53 0.23625382006275686 1103 54-55 0.4427787524105611 1103 56-57 0.32048618781635696 1103 58-59 0.23351311806364805 1103 60-61 0.5805479200984323 1103 62-63 0.613488718539088 1103 64-65 0.1540847356748145 1103 66-67 0.15098119875098348 1103 68-69 0.47298623740415735 1103 70-71 0.4070600473860182 1103 72-73 0.27049780289632963 1103 74-75 0.26214479102092625 1103 76-77 0.09240674446422759 1103 78-79 -0.02101758593465064 1103 80-81 -0.013810680375513584 1103 82-83 0.14043635579437463 1103 84-85 0.15840304932560656 1103 86-87 0.24097272191851715 1103 88-89 0.5952508809460504 1103 90-91 0.2192973896527839 1103 92-93 -0.05092382762293468 1103 94-95 -0.025950102330607905 1103 96-97 0.07618247794036392 1103 98-99 -0.0549983442592783 1103 100 0.1943073808313649 1104 1 0.30343645701727695 1104 2 3.4726190494219544 1104 3 2.1072488892037136 1104 4 1.0168312220283227 1104 5 0.2847214045842179 1104 6 0.16635439797070717 1104 7 0.2432844252324884 1104 8 0.2661044499044607 1104 9 0.2076091834859639 1104 10-11 0.4320835462604151 1104 12-13 0.4813242457150224 1104 14-15 0.7668317575978705 1104 16-17 0.7247570074917746 1104 18-19 0.5757889133297027 1104 20-21 0.5240123563905428 1104 22-23 0.47750912663101275 1104 24-25 0.6409919173343539 1104 26-27 0.45922432091845167 1104 28-29 0.4854956218649278 1104 30-31 0.3528931142990004 1104 32-33 0.5850985908473518 1104 34-35 0.7030873147873464 1104 36-37 0.5303637709392106 1104 38-39 0.5123183105731144 1104 40-41 0.550334570781466 1104 42-43 0.4534853264701013 1104 44-45 0.5626359118389033 1104 46-47 0.31917015201265997 1104 48-49 0.28453671749451814 1104 50-51 0.07472130134469523 1104 52-53 0.2979916524309658 1104 54-55 0.32336764775590865 1104 56-57 0.49793724678603724 1104 58-59 0.34853004955622424 1104 60-61 0.24860350348352256 1104 62-63 0.2551682424535713 1104 64-65 0.43652607656886744 1104 66-67 0.08768146123921383 1104 68-69 0.35959132286311046 1104 70-71 0.43604195373182364 1104 72-73 0.40772704929463544 1104 74-75 0.2828412865025207 1104 76-77 0.1763278376976416 1104 78-79 0.18982458049491058 1104 80-81 0.3747360164806395 1104 82-83 0.36350826955397153 1104 84-85 0.10391971031504355 1104 86-87 0.40379356869351923 1104 88-89 0.4453109927540133 1104 90-91 0.19933325636486643 1104 92-93 0.34353686927329363 1104 94-95 0.22860036153217678 1104 96-97 0.2459642720774582 1104 98-99 0.32796697122752505 1104 100 0.39574605676860486 1105 1 0.5979351864645182 1105 2 3.7043703047767167 1105 3 2.2056731606116173 1105 4 0.9578974326199088 1105 5 0.1985933280025094 1105 6 0.04089389858470582 1105 7 -0.022259075494993397 1105 8 0.06669251116658614 1105 9 0.11999980854928793 1105 10-11 0.06498498622021032 1105 12-13 0.267722441408516 1105 14-15 0.11691261095540995 1105 16-17 0.19175100529515987 1105 18-19 0.16635277937656667 1105 20-21 0.12809534962642033 1105 22-23 0.25210354268333646 1105 24-25 0.043848330687517034 1105 26-27 0.17361743440090294 1105 28-29 0.14714357135082423 1105 30-31 0.10379283587164423 1105 32-33 0.222278774277072 1105 34-35 0.2454410998998 1105 36-37 0.35930724653933055 1105 38-39 0.1793037974967575 1105 40-41 0.3107077120661259 1105 42-43 0.18654360978512585 1105 44-45 0.040023314926330045 1105 46-47 0.10845191481848815 1105 48-49 -0.14705708658502914 1105 50-51 0.14323388326190667 1105 52-53 -0.002431257236182205 1105 54-55 -0.02292485091680163 1105 56-57 0.08534904772871243 1105 58-59 0.05502526927877227 1105 60-61 0.22306579825986717 1105 62-63 -0.13872540397206734 1105 64-65 -0.028946094880772932 1105 66-67 -0.09787192831996094 1105 68-69 -0.12845291750034704 1105 70-71 0.16014557571907062 1105 72-73 0.06526343330552464 1105 74-75 0.16538709327312162 1105 76-77 -0.2532030912446146 1105 78-79 -0.06471151576719336 1105 80-81 0.004464114136901287 1105 82-83 0.2336332579021665 1105 84-85 0.4764632069897239 1105 86-87 0.5938050381171678 1105 88-89 0.45324536171280627 1105 90-91 0.2569894006121203 1105 92-93 0.13496256321394995 1105 94-95 0.3308940436224539 1105 96-97 0.36460593567074895 1105 98-99 0.178962744157257 1105 100 0.11932889397168722 1106 1 0.41155119684219343 1106 2 3.808262323812876 1106 3 2.2903727315947577 1106 4 1.0012816644921898 1106 5 0.3827560418271716 1106 6 0.14468237530135752 1106 7 0.28223153536561796 1106 8 0.4808696036982525 1106 9 0.04118261349118768 1106 10-11 0.3131446680131802 1106 12-13 0.20631989231382875 1106 14-15 0.3761298310199521 1106 16-17 0.22825358017774988 1106 18-19 0.3679535827086724 1106 20-21 0.12511020501882086 1106 22-23 0.19483487185414106 1106 24-25 0.2245257709650872 1106 26-27 0.15535295996608767 1106 28-29 0.22589043577681878 1106 30-31 6.891060037901298E-4 1106 32-33 0.042073135323278166 1106 34-35 -0.08811169105411665 1106 36-37 -0.047730310018216926 1106 38-39 0.19157356235800194 1106 40-41 0.312409322637599 1106 42-43 0.37614913308409825 1106 44-45 0.19942769261745497 1106 46-47 -0.048221289992127936 1106 48-49 -0.167283004204414 1106 50-51 0.039177759417484026 1106 52-53 0.14323813409127695 1106 54-55 0.34553635392506976 1106 56-57 -0.02495966467370181 1106 58-59 -0.0882724168434379 1106 60-61 0.08946690166934701 1106 62-63 0.16154439214724903 1106 64-65 0.16437913740567467 1106 66-67 0.13888735336985292 1106 68-69 5.168559568744513E-4 1106 70-71 0.21120659144969522 1106 72-73 0.1760410165887194 1106 74-75 -0.21624449924681954 1106 76-77 0.15535829567544823 1106 78-79 0.3021047337346303 1106 80-81 0.08178971370554322 1106 82-83 -0.09825699505367069 1106 84-85 0.1099194886873498 1106 86-87 0.028684733663681072 1106 88-89 0.2185383669407166 1106 90-91 -0.054758940757139385 1106 92-93 -0.0017689769487461149 1106 94-95 0.02116673111219214 1106 96-97 0.02356879328247885 1106 98-99 -0.07419627340195944 1106 100 0.030475908436354615 1107 1 0.6459249671275664 1107 2 3.779107265652293 1107 3 2.2535521243321632 1107 4 1.0966563634580453 1107 5 0.5815605624548965 1107 6 0.36201721970802936 1107 7 0.22952104757226266 1107 8 0.267855484606784 1107 9 0.4094516475060743 1107 10-11 0.41282828703381824 1107 12-13 0.2693796584122694 1107 14-15 0.24412340895828777 1107 16-17 0.16685245316888597 1107 18-19 0.3888016726175323 1107 20-21 0.4602812238914993 1107 22-23 0.6004203340694332 1107 24-25 0.45355569336518187 1107 26-27 0.5410229478460167 1107 28-29 0.4872589015651698 1107 30-31 0.5517763704888452 1107 32-33 0.688999439017536 1107 34-35 0.8521497561133984 1107 36-37 0.841498963879566 1107 38-39 0.3379332135519135 1107 40-41 0.561071393122873 1107 42-43 0.622619360217314 1107 44-45 0.6659204284472011 1107 46-47 0.5296147270704097 1107 48-49 0.15252543576977473 1107 50-51 0.49807577770776845 1107 52-53 0.30351618732512975 1107 54-55 0.2982307078625226 1107 56-57 0.15521812254829115 1107 58-59 0.2621844467349774 1107 60-61 0.0017341412846576532 1107 62-63 0.41875937380974193 1107 64-65 0.3682520498421269 1107 66-67 0.12111846888825539 1107 68-69 0.23895360337152027 1107 70-71 0.5035894439154163 1107 72-73 0.38066356306209315 1107 74-75 0.30117613005226573 1107 76-77 0.5703269723844571 1107 78-79 0.35200753709047206 1107 80-81 0.2928067762419424 1107 82-83 0.43033534569336496 1107 84-85 0.35859729951985386 1107 86-87 0.1678569488027435 1107 88-89 -0.07741164171647341 1107 90-91 0.15304512340051346 1107 92-93 0.20103162433251853 1107 94-95 0.28428040789990305 1107 96-97 0.1805984323563159 1107 98-99 0.4704354811745439 1107 100 0.3753744618984456 1108 1 0.34000649363416 1108 2 3.5412737498896227 1108 3 2.0096246923938352 1108 4 0.7284596192395085 1108 5 0.2389854365010251 1108 6 -0.15217733535845923 1108 7 0.041708103628529614 1108 8 -0.04988596256245614 1108 9 -0.13811195046623936 1108 10-11 -0.10627439351300438 1108 12-13 0.038455168632189896 1108 14-15 -0.21882402810359736 1108 16-17 -0.3499582162303412 1108 18-19 -0.3123383166695888 1108 20-21 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1.3907615114476322 2202 86-87 1.2973340240378164 2202 88-89 1.243997683517609 2202 90-91 1.3547993008153334 2202 92-93 1.2662377312632422 2202 94-95 1.04165155887328 2202 96-97 0.9667141993482993 2202 98-99 0.9986104947459431 2202 100 1.1894463169995078 2203 1 0.5451629687498603 2203 2 4.089842372320081 2203 3 2.4209140595762904 2203 4 1.2424829538849167 2203 5 0.7211577062596106 2203 6 0.6640155174987541 2203 7 0.40565175806482046 2203 8 0.5597364258565918 2203 9 0.7799798518351224 2203 10-11 0.8303100961706065 2203 12-13 0.8288780660873911 2203 14-15 0.8668952529783738 2203 16-17 1.0900322680036822 2203 18-19 1.2320036241645056 2203 20-21 1.2210359099617563 2203 22-23 1.1234610951887376 2203 24-25 1.1230383975603644 2203 26-27 1.1057318740556425 2203 28-29 1.1119217562420545 2203 30-31 1.1836226681836663 2203 32-33 1.3882458318123128 2203 34-35 1.468033846050787 2203 36-37 1.2090992483802694 2203 38-39 1.1672008240256986 2203 40-41 0.8738123895945833 2203 42-43 0.9739907175493983 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0.9221644555996278 2210 82-83 0.7752459906040094 2210 84-85 0.6743315652541249 2210 86-87 1.1514622324080293 2210 88-89 0.7645593502545189 2210 90-91 0.7649073352627269 2210 92-93 1.154825978126869 2210 94-95 0.7936303172498178 2210 96-97 0.6174412191991046 2210 98-99 0.8602308709699358 2210 100 0.2614402479642308 2211 1 0.14213719448733286 2211 2 3.742448538829631 2211 3 2.0801879657221036 2211 4 0.735159936387852 2211 5 0.19392803711745188 2211 6 -0.07104989532629702 2211 7 -0.07710638692402938 2211 8 -0.10773650082126807 2211 9 -0.15838264327903318 2211 10-11 -0.15090297259908425 2211 12-13 -0.014131153787069195 2211 14-15 -0.07299162536986614 2211 16-17 -0.17314801388076262 2211 18-19 0.175511774901409 2211 20-21 -0.059981725059969904 2211 22-23 -0.2614100334986347 2211 24-25 0.12421329926868196 2211 26-27 -0.1396248500148971 2211 28-29 -0.02144223336547668 2211 30-31 -0.4242429653665525 2211 32-33 -0.42825337495659 2211 34-35 -0.21678210134304265 2211 36-37 -0.3535246221276296 2211 38-39 -0.12177441336882566 2211 40-41 -0.19445146895736798 2211 42-43 -0.018819619746253125 2211 44-45 -0.39658716110956505 2211 46-47 0.34112750087827237 2211 48-49 0.08991743053881862 2211 50-51 0.029989280113071004 2211 52-53 -0.008272145774682826 2211 54-55 0.12290027023699679 2211 56-57 0.03802602502832286 2211 58-59 0.23381118065678663 2211 60-61 -0.27082202143544976 2211 62-63 -0.18335869634110935 2211 64-65 -0.12015577790996446 2211 66-67 -0.09002533569817217 2211 68-69 -0.10085303807446167 2211 70-71 -0.12986532232623205 2211 72-73 -0.02183281730314235 2211 74-75 -0.2157871131404896 2211 76-77 0.10842187687280358 2211 78-79 -0.34911571731147006 2211 80-81 -0.18212433393507155 2211 82-83 -0.8849792122441436 2211 84-85 -0.255491253093286 2211 86-87 -0.11291928771119686 2211 88-89 0.4378165120362709 2211 90-91 -0.11808653789507773 2211 92-93 0.10312867724924146 2211 94-95 -0.09832199273528275 2211 96-97 -0.13680617275320728 2211 98-99 -0.065121008480304 2211 100 -0.5737311380268295 2212 1 1.037133332157918 2212 2 4.304494715123514 2212 3 2.54524675895874 2212 4 1.375619982735806 2212 5 0.7922972758094105 2212 6 0.6242895605143985 2212 7 0.26236661968484754 2212 8 0.5930445480157474 2212 9 0.6087103101155265 2212 10-11 0.36495832686019014 2212 12-13 0.5114889646577012 2212 14-15 0.6198673566917563 2212 16-17 0.8838428023581884 2212 18-19 0.561229739024661 2212 20-21 0.48972216301829974 2212 22-23 0.4777454038028779 2212 24-25 0.09475660027288768 2212 26-27 0.812919995921078 2212 28-29 0.8420716281056428 2212 30-31 0.656119235302917 2212 32-33 0.5716607955008897 2212 34-35 0.6672106889753806 2212 36-37 0.4768676188817267 2212 38-39 0.7689903278551071 2212 40-41 0.45493484981355437 2212 42-43 0.6018223852318556 2212 44-45 1.0797753828780756 2212 46-47 0.7332140170571364 2212 48-49 0.8920803350105331 2212 50-51 0.8616517983518435 2212 52-53 0.7917621860423338 2212 54-55 0.341808518456034 2212 56-57 1.0123801577207985 2212 58-59 0.8552170685634053 2212 60-61 0.38703567318304266 2212 62-63 0.6113542038391344 2212 64-65 0.3719736530401647 2212 66-67 0.4427872984104937 2212 68-69 0.5227500861208796 2212 70-71 1.2317259573922428 2212 72-73 1.3982684615570449 2212 74-75 0.814742455136912 2212 76-77 0.24464194381985038 2212 78-79 0.1797659237493825 2212 80-81 0.5557093284115098 2212 82-83 0.8082831686673693 2212 84-85 0.4641036314659779 2212 86-87 0.5937754166060287 2212 88-89 1.0595828840215127 2212 90-91 0.832964874000897 2212 92-93 0.6761438690782704 2212 94-95 0.5377521639894631 2212 96-97 0.40449500311152775 2212 98-99 0.7415307810656557 2212 100 0.9736725183116839 2213 1 0.17162036305196793 2213 2 -25.988699002153975 2213 3 -14.773785254150251 2213 4 -6.055917525757064 2213 5 -1.6868002822418262 2213 6 -0.5425303107937012 2213 7 -0.5080662167325585 2213 8 -0.3674286544941481 2213 9 -0.8060612817473114 2213 10-11 -0.6103823869402163 2213 12-13 -0.17105769614722277 2213 14-15 -0.8167658713966475 2213 16-17 -0.6960800624353851 2213 18-19 -0.8731054194422256 2213 20-21 -0.8134731529163162 2213 22-23 -1.092795341970664 2213 24-25 -0.6137982136833884 2213 26-27 -0.541032683827936 2213 28-29 -0.905430470131833 2213 30-31 -0.5942404625508928 2213 32-33 -1.0285849980325494 2213 34-35 -0.6498130111165423 2213 36-37 -1.2862113947468217 2213 38-39 -1.3460830105406458 2213 40-41 -0.8582920395993412 2213 42-43 -1.320552819596088 2213 44-45 -1.4034886753130493 2213 46-47 -1.7869290627558172 2213 48-49 -1.2107333015347663 2213 50-51 -1.1647360358674064 2213 52-53 -1.4910402599030732 2213 54-55 -1.617745456205249 2213 56-57 -0.9383911943434669 2213 58-59 -1.3423369860307304 2213 60-61 -1.112274905930505 2213 62-63 -0.8141683572095886 2213 64-65 -0.5283900414564542 2213 66-67 -1.0498488871936011 2213 68-69 -1.5375192876051855 2213 70-71 -1.386854435078078 2213 72-73 -0.9401972272643491 2213 74-75 -0.598630311738912 2213 76-77 -1.1577400553008914 2213 78-79 -1.3327535598843632 2213 80-81 -0.8826624393036084 2213 82-83 -0.9741795626383372 2213 84-85 -0.8957740712637268 2213 86-87 -0.9973580312977326 2213 88-89 -0.44709150948794374 2213 90-91 -0.5835852239168524 2213 92-93 -1.1747214040922636 2213 94-95 -1.229513332993072 2213 96-97 -1.2705879272269875 2213 98-99 -0.7193820521544403 2213 100 -1.2462627360746303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 3.0 6 4.0 7 2.0 8 7.0 9 10.0 10 55.0 11 249.0 12 559.0 13 955.0 14 1450.0 15 1822.0 16 2101.0 17 2256.0 18 2220.0 19 2451.0 20 2970.0 21 3829.0 22 4239.0 23 4314.0 24 3717.0 25 3428.0 26 3547.0 27 4064.0 28 4696.0 29 5613.0 30 6768.0 31 8622.0 32 10696.0 33 14771.0 34 20566.0 35 29875.0 36 46874.0 37 72508.0 38 51721.0 39 151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.73688864292007 20.0987267933194 10.538385447976005 28.625999115784516 2 13.223598174490913 23.321278923910434 33.570084603272036 29.885038298326617 3 18.06362300468595 27.7631670230009 28.3717732831312 25.801436689181944 4 12.195321936189872 20.266669189553983 32.007556047518584 35.53045282673756 5 11.61835161399331 38.40549272510793 34.12993427805342 15.846221382845341 6 32.05966702317966 34.196851958770694 17.308863291466718 16.434617726582935 7 24.10128451017009 33.922487760561246 22.45306112148589 19.52316660778277 8 27.17694081572779 33.01145451161382 20.845255258002542 18.966349414655845 9 26.675520756109794 14.990015489398752 21.69367584568647 36.64078790880498 10-11 23.105849098864628 27.496380000567843 26.792885557543006 22.604885343024524 12-13 26.324406686625174 25.09093318106823 27.50945610443199 21.07520402787461 14-15 25.20383424478407 25.143736858626024 25.96475233094258 23.687676565647326 16-17 20.842546882639624 28.309228269955682 29.79480466223996 21.05342018516474 18-19 22.145030210027112 26.54902288581067 32.04186978855347 19.26407711560875 20-21 21.471929196505876 26.128974771839463 31.94767959904935 20.451416432605313 22-23 20.43382127976331 26.052302053664643 29.99750822443643 23.51636844213561 24-25 21.35698797555687 26.9144096195545 28.991129509166175 22.73747289572245 26-27 22.247995395520093 24.78976291659111 30.409593717723304 22.55264797016549 28-29 21.636675303212133 24.29686797755024 32.179042999273015 21.887413719964613 30-31 22.831657998240033 26.038713014625408 30.289448700682225 20.840180286452338 32-33 19.65151498428806 25.999832868465905 31.364439770367582 22.984212376878457 34-35 19.225881321564508 26.564682806227513 31.870429410707356 22.339006461500617 36-37 22.96641664551184 25.687584455064783 30.089417908494465 21.256580990928914 38-39 23.282098504582123 24.845790944238765 29.936739597984246 21.935370953194873 40-41 21.153006214098642 25.935228940891164 30.392319178559546 22.519445666450647 42-43 23.199925575058813 25.130874366914963 29.994039697506764 21.67516036051946 44-45 24.399631658451515 24.454819818541328 28.906454491843192 22.239094031163965 46-47 22.049443106680986 25.776205051542 31.397965443125603 20.776386398651407 48-49 23.46100530043416 25.744244983827446 30.085996035319408 20.708753680418987 50-51 21.580111873863558 26.293511978262345 29.656351372875555 22.47002477499854 52-53 21.033664840381405 26.228168951888897 27.719950668218534 25.018215539511164 54-55 22.004185505911593 27.171566315982044 26.643252645905253 24.180995532201113 56-57 23.020747751682986 24.869863326925714 27.88307332280991 24.226315598581387 58-59 22.12476878462317 24.15426027637038 29.994685791509568 23.72628514749688 60-61 23.02594128008099 25.83720589186061 28.444106641352462 22.692746186705943 62-63 20.34731158378119 25.99285948578205 29.244884313577067 24.41494461685969 64-65 19.55339683340693 26.329559073992304 29.949536365356717 24.167507727244057 66-67 22.789227753500573 25.3403583915119 28.462209297741246 23.408204557246286 68-69 23.439897678042257 24.804716146461708 28.197994880747924 23.557391294748115 70-71 21.462929201809224 25.721828653616868 28.482030771948196 24.33321137262571 72-73 23.09716998241588 25.122418565041514 28.64949258391881 23.130918868623787 74-75 23.82333413762161 24.502721175333267 27.815284946719842 23.85865974032528 76-77 21.72500634726894 25.431969104175632 30.36517691139528 22.477847637160146 78-79 23.03444404321314 25.429427004899402 29.717468332074446 21.818660619813013 80-81 21.196701147975276 25.76510659770405 29.593635675539296 23.44455657878138 82-83 20.31120158565218 25.736415284450803 28.15131814433282 25.801064985564192 84-85 21.235993680007823 26.060191302757268 27.330497115951673 25.373317901283237 86-87 22.141979756823282 24.577215940358474 28.240319526198025 25.040484776620218 88-89 21.55590805453152 23.873466182698888 29.94314114430418 24.62748461846541 90-91 22.107239941722224 25.034846831618847 28.81832974878746 24.039583477871474 92-93 19.894232118558417 25.459659833590603 29.08033344948669 25.56577459836429 94-95 19.24944735788189 25.77992702916626 29.631708570761845 25.33891704219001 96-97 21.71105119593838 24.79337148983807 28.691957176418647 24.803620137804895 98-99 22.106018321429136 24.400296422433502 28.220834712959018 25.272850543178343 100 20.481608222949 25.270857272738752 29.759259025418917 24.488275478893335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23.0 1 20.0 2 15.0 3 16.0 4 24.5 5 122.5 6 455.0 7 945.0 8 1409.5 9 1460.0 10 900.5 11 373.5 12 220.0 13 184.0 14 174.0 15 187.0 16 200.5 17 212.5 18 232.5 19 265.0 20 295.0 21 354.0 22 437.5 23 548.0 24 688.0 25 901.5 26 1243.0 27 1692.0 28 2187.0 29 2728.0 30 3366.5 31 4054.5 32 4770.5 33 5390.0 34 5975.5 35 6538.5 36 7107.5 37 7770.0 38 8297.0 39 8648.0 40 8771.5 41 8643.0 42 8574.5 43 8650.5 44 8638.0 45 8516.5 46 8801.0 47 9994.0 48 11211.5 49 14692.5 50 27624.0 51 28384.5 52 14430.5 53 8855.0 54 7875.0 55 7416.0 56 6840.0 57 5964.0 58 5154.0 59 4547.0 60 4052.5 61 3495.0 62 2895.5 63 2413.5 64 2054.0 65 1752.0 66 1488.5 67 1227.0 68 954.5 69 738.5 70 565.0 71 447.5 72 338.0 73 237.0 74 174.0 75 111.0 76 62.0 77 41.0 78 32.5 79 15.0 80 5.5 81 4.5 82 3.0 83 1.5 84 2.0 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8561481845874406 2 5.476194981048064 3 0.0 4 0.006622140654267496 5 0.15167855498584124 6 0.05013906495373962 7 0.03468740342711546 8 0.03941750389444939 9 0.040048183956760575 10-11 0.04067886401907177 12-13 0.040048183956760575 14-15 0.04178255412811635 16-17 0.03248002320902629 18-19 0.027592252726114572 20-21 0.022546812227625047 22-23 0.02365050233666963 24-25 0.017501371729135524 26-27 0.011194571106023626 28-29 0.01718603169797993 30-31 0.02112778208742487 32-33 7.883500778889876E-4 34-35 0.0028380602804003556 36-37 0.003626410358289343 38-39 0.005045440498489521 40-41 0.005203110514067319 42-43 0.006306800623111901 44-45 0.006622140654267496 46-47 0.014663311448735172 48-49 0.021600792134158265 50-51 0.020339432009535884 52-53 0.025542542523603204 54-55 0.022546812227625047 56-57 0.014978651479890767 58-59 0.013717291355268385 60-61 0.01150991113717922 62-63 0.017028361682402134 64-65 0.018289721807024515 66-67 0.019708751947224695 68-69 0.02506953247686981 70-71 0.023965842367825226 72-73 0.022231472196469454 74-75 0.021285452103002665 76-77 0.016555351635668743 78-79 0.012140591199490411 80-81 0.011982921183912613 82-83 0.00772583076331208 84-85 0.009144860903512258 86-87 0.0074104907321564835 88-89 0.005360780529645117 90-91 0.005045440498489521 92-93 0.0026803902648225584 94-95 0.0015767001557779753 96-97 9.460200934667853E-4 98-99 9.460200934667853E-4 100 0.1091076507798359 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 317118.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.95908084901897 #Duplication Level Percentage of deduplicated Percentage of total 1 87.62991308309655 57.79988521840471 2 8.513824056522418 11.231280185569698 3 2.1274999944632755 4.209838324232718 4 0.704400289404354 1.858463825555766 5 0.310079114624907 1.0226266695568234 6 0.17244077843274677 0.6824421147787989 7 0.10380455851763767 0.47927972874331093 8 0.07061299341777844 0.3726054513467595 9 0.05105068338174814 0.30305305373169555 >10 0.25505601933289346 3.130242413571365 >50 0.02922206372771521 1.3776602660660888 >100 0.01724580810160221 2.3628769425784677 >500 0.008143853825756598 4.036923752123897 >1k 0.005748602700534069 6.966663657391021 >5k 9.581004500890116E-4 4.166158396348903 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7413 2.3376156509564265 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5772 1.8201426598300947 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2655 0.8372277827181048 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2408 0.759338795022673 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2268 0.7151911906608897 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2194 0.6918560283553756 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1938 0.6111289803795433 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1861 0.5868477979805624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1709 0.5389161132449121 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1641 0.5174729911263315 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1638 0.5165269710328647 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1359 0.4285471023404537 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1201 0.3787233774178697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1176 0.3708398766389798 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 968 0.30524915015861603 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 882 0.2781299074792349 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 875 0.2759225272611457 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 855 0.2696157266380338 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 844 0.26614698629532224 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 823 0.25952484564105477 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 798 0.2516413448621649 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 772 0.2434425040521194 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 751 0.23682036339785192 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 747 0.2355590032732295 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 718 0.22641414236971727 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 708 0.22326074205816132 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 673 0.2122238409677155 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 670 0.21127782087424868 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 634 0.1999255797526473 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 538 0.16965293676171014 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 520 0.16397681620090945 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 484 0.15262457507930802 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 438 0.13811893364615063 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 363 0.114468431309481 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.153400311555951E-4 0.0 9 0.0 0.0 0.0 3.153400311555951E-4 0.0 10-11 0.0 0.0 0.0 3.153400311555951E-4 0.0 12-13 0.0 0.0 0.0 4.730100467333926E-4 0.0 14-15 0.0 0.0 0.0 6.306800623111902E-4 0.0 16-17 0.0 0.0 0.0 6.306800623111902E-4 0.0 18-19 0.0 0.0 0.0 6.306800623111902E-4 0.0 20-21 0.0 0.0 0.0 6.306800623111902E-4 0.0 22-23 0.0 0.0 0.0 0.0014190301402001778 0.0 24-25 0.0 0.0 0.0 0.003153400311555951 0.0 26-27 0.0 0.0 0.0 0.004572430451756129 0.0 28-29 0.0 0.0 0.0 0.008987190887934461 0.0 30-31 0.0 0.0 0.0 0.02160079213415826 0.0 32-33 0.0 0.0 0.0 0.051715765109517586 0.0 34-35 0.0 0.0 0.0 0.09712472959592328 0.0 36-37 0.0 0.0 0.0 0.1597197257803089 0.0 38-39 0.0 0.0 0.0 0.25779047546969897 0.0 40-41 0.0 0.0 0.0 0.39654008917816086 0.0 42-43 0.0 0.0 0.0 0.49634520903890667 0.0 44-45 0.0 0.0 0.0 0.6092369401926097 0.0 46-47 0.0 0.0 0.0 0.7544510245397613 0.0 48-49 0.0 0.0 0.0 0.9006111289803795 0.0 50-51 0.0 0.0 0.0 1.047401913483309 0.0 52-53 0.0 0.0 0.0 1.2323488417560657 0.0 54-55 0.0 0.0 0.0 1.4169804299976665 0.0 56-57 0.0 0.0 0.0 1.611072219173935 0.0 58-59 0.0 0.0 0.0 1.8436354921511866 0.0 60-61 0.0 0.0 0.0 2.073991384910349 0.0 62-63 0.0 0.0 0.0 2.3147534986976455 0.0 64-65 0.0 0.0 0.0 2.6100694378748606 0.0 66-67 0.0 0.0 0.0 2.9301395694977894 0.0 68-69 0.0 0.0 0.0 3.314381397460882 0.0 70-71 0.0 0.0 0.0 3.8419452695841927 0.0 72-73 0.0 0.0 0.0 4.295088894354783 0.0 74-75 0.0 0.0 0.0 4.725685706897748 0.0 76-77 0.0 0.0 0.0 5.221873245921076 0.0 78-79 0.0 0.0 0.0 5.760789359165988 0.0 80-81 0.0 0.0 0.0 6.320833254498326 0.0 82-83 0.0 0.0 0.0 7.026721914240126 0.0 84-85 0.0 0.0 0.0 7.7073833714894775 0.0 86-87 0.0 0.0 0.0 8.359979565965983 0.0 88 0.0 0.0 0.0 8.933898422669165 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGCGG 45 0.0010183809 41.73151 9 CCTTGCG 50 0.0017094468 37.55836 8 TAGACAA 75 3.2521575E-4 31.36294 5 GTTATAG 65 0.0046403534 30.635431 1 TTAGACA 100 5.277437E-5 28.226648 4 AAGTACT 175 9.419455E-8 25.593906 2 AGTACTC 175 1.623448E-7 24.19427 3 CTGGACA 150 3.257829E-5 21.954058 4 AAAGTAC 185 4.3461223E-6 21.527597 1 TTTAGAC 110 0.0030380117 21.383823 3 ACATTGC 110 0.0030744118 21.339977 8 GAGTACT 2015 0.0 20.73468 12-13 CTCCAAC 145 4.468569E-4 20.599686 1 TCTGTTC 160 5.4432654E-5 20.539728 8 CAGGACT 115 0.003930835 20.454092 4 CTTAGCA 125 0.0045990585 19.90637 2 TAGACAG 125 0.006366329 18.817764 5 CAGTTAA 160 8.670518E-4 18.668463 1 GTACTTT 2245 0.0 18.608948 14-15 GGTGTCC 130 0.008077854 18.056904 8 >>END_MODULE