FastQCFastQC Report
Wed 25 May 2016
SRR1295120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850514
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67350.019.3432751
TACATGG69850.018.1249732
GTATCAA43950.017.923121
GTGTAAG10900.017.2331371
TCAACGC46450.017.1641964
ATCAACG46950.016.8639053
ACATGGG72550.016.8246783
GTATTAG11700.016.57271
CAACGCA46100.016.1106725
CATGGGG36550.015.946294
TATACGG1900.00272419715.7407695
TATCAAC48750.015.6564542
AACGCAG47600.015.6465586
TAGCGCA2150.003228535615.3445444
GTATATA20250.015.26069451
TATTACG2856.424892E-414.8782092
CGCAGAG50550.014.8406888
GTCTTAC8700.014.62612151
GTATAAG10850.014.5202051
GTATAGC6708.913048E-1114.4701921
Produced by FastQC (version 0.11.5)