##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295120_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2850514 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.56050207085459 33.0 27.0 33.0 15.0 33.0 2 22.6287097695363 27.0 6.0 33.0 2.0 33.0 3 24.94383714656374 27.0 15.0 33.0 15.0 33.0 4 25.42128051291802 33.0 15.0 37.0 2.0 37.0 5 27.178430626897466 33.0 15.0 37.0 2.0 37.0 6 28.147946651025045 33.0 22.0 37.0 6.0 37.0 7 27.84611827901915 33.0 15.0 37.0 6.0 37.0 8 28.52323756347101 33.0 22.0 37.0 6.0 37.0 9 29.609588656642277 37.0 27.0 37.0 15.0 37.0 10-11 29.182198368434605 35.0 24.5 37.0 10.5 37.0 12-13 28.036167161431237 33.0 18.5 37.0 4.0 37.0 14-15 29.77242911278457 35.0 27.0 37.0 10.5 40.0 16-17 30.19107781964937 37.0 27.0 40.0 6.0 40.0 18-19 30.323004202049173 37.0 27.0 40.0 10.5 40.0 20-21 29.85154958018098 35.0 27.0 40.0 6.0 40.0 22-23 30.586685769654174 37.0 27.0 40.0 15.0 40.0 24-25 30.722067669199312 37.0 27.0 40.0 15.0 40.0 26-27 29.29608852999845 35.0 24.5 40.0 4.0 40.0 28-29 30.006838942029404 37.0 27.0 40.0 10.5 40.0 30-31 30.704546267795912 37.0 27.0 40.0 15.0 40.0 32-33 29.490900062234388 33.0 27.0 40.0 6.0 40.0 34-35 29.181310809208444 33.0 22.0 37.0 6.0 40.0 36-37 29.44567611315012 33.0 27.0 37.0 6.0 40.0 38-39 29.567691826807376 33.0 24.5 37.0 6.0 40.0 40-41 28.716409040615133 33.0 22.0 37.0 6.0 40.0 42-43 29.390075088212164 33.0 22.0 37.0 6.0 40.0 44-45 29.221477073959292 33.0 22.0 37.0 6.0 40.0 46-47 28.874462114551974 33.0 22.0 37.0 6.0 40.0 48-49 28.783727952221952 33.0 22.0 37.0 6.0 40.0 50-51 28.710991947417202 33.0 22.0 37.0 6.0 40.0 52-53 28.90503572338182 33.0 22.0 37.0 6.0 40.0 54-55 28.874827662660138 33.0 22.0 37.0 6.0 40.0 56-57 28.851275419099856 33.0 22.0 37.0 6.0 40.0 58-59 28.75967106283288 33.0 22.0 37.0 6.0 40.0 60-61 28.740022501205047 33.0 22.0 37.0 10.5 40.0 62-63 28.666991637297695 33.0 22.0 37.0 10.5 40.0 64-65 28.20795354802678 33.0 22.0 37.0 6.0 40.0 66-67 28.41848663083219 33.0 22.0 37.0 10.5 40.0 68-69 28.00482842743449 33.0 22.0 37.0 6.0 38.5 70-71 28.16003341853434 33.0 22.0 37.0 10.5 37.0 72-73 28.18061637304711 33.0 22.0 37.0 10.5 37.0 74-75 28.263564395754592 33.0 22.0 37.0 15.0 37.0 76-77 28.059622229534742 33.0 22.0 37.0 15.0 37.0 78-79 27.598966361856142 33.0 22.0 37.0 10.5 37.0 80-81 27.410639800400908 33.0 22.0 35.0 10.5 37.0 82-83 27.5086489664671 33.0 22.0 33.0 15.0 37.0 84-85 27.43047692451256 30.0 22.0 33.0 15.0 37.0 86-87 27.221661602082992 27.0 22.0 33.0 15.0 37.0 88-89 27.158764349166503 27.0 22.0 33.0 15.0 37.0 90-91 27.319290310449276 27.0 22.0 33.0 15.0 37.0 92-93 27.25000578842974 27.0 22.0 33.0 15.0 37.0 94-95 27.12150931375885 27.0 22.0 33.0 15.0 37.0 96-97 27.19947086735936 27.0 22.0 33.0 15.0 37.0 98-99 27.25139132802014 27.0 22.0 33.0 15.0 37.0 100 26.9274927258733 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.303354824199236 1101 2 11.368762791895463 1101 3 8.49678889664591 1101 4 12.813159282670572 1101 5 10.822893662525896 1101 6 10.077831139244157 1101 7 9.602816764929909 1101 8 9.163859724786665 1101 9 7.482590577223164 1101 10-11 7.9086608914231 1101 12-13 9.379393721430084 1101 14-15 8.272198930152303 1101 16-17 8.50832815206616 1101 18-19 8.329469561022702 1101 20-21 7.375816360496366 1101 22-23 7.5765616211945535 1101 24-25 7.50473452768232 1101 26-27 8.674171545678579 1101 28-29 8.397276167323174 1101 30-31 5.660343795677893 1101 32-33 7.10095985134274 1101 34-35 8.988945211117407 1101 36-37 8.406455899831915 1101 38-39 8.34321098705253 1101 40-41 9.165076266573514 1101 42-43 8.838269895881421 1101 44-45 8.762220192228494 1101 46-47 7.688934905885496 1101 48-49 5.723010347553519 1101 50-51 7.098084578895449 1101 52-53 6.1422041520528445 1101 54-55 4.230404006564864 1101 56-57 6.459051061578396 1101 58-59 6.481312930051406 1101 60-61 6.941138404746074 1101 62-63 6.557845815086672 1101 64-65 6.995394267633163 1101 66-67 6.025684478448092 1101 68-69 6.9908859681086 1101 70-71 6.56092328947296 1101 72-73 5.699282030651812 1101 74-75 5.0861984756796765 1101 76-77 5.213762052334658 1101 78-79 5.330545585348368 1101 80-81 5.20929216689402 1101 82-83 4.795143158807289 1101 84-85 4.564379761517511 1101 86-87 4.2973293413229605 1101 88-89 4.228912745525534 1101 90-91 4.067182599825561 1101 92-93 4.0024391301057065 1101 94-95 3.860803442363334 1101 96-97 4.039547039985674 1101 98-99 3.738956601256781 1101 100 4.055422574817342 1102 1 6.66905785993848 1102 2 10.437667643621971 1102 3 8.11555117780238 1102 4 12.51406168913012 1102 5 10.453590563608625 1102 6 9.547668565229994 1102 7 6.6490855839081675 1102 8 7.894597679864045 1102 9 6.423382888062953 1102 10-11 7.544858153571955 1102 12-13 9.076387891137706 1102 14-15 6.320954223924748 1102 16-17 7.508885391913207 1102 18-19 7.647705575974054 1102 20-21 7.124384037122553 1102 22-23 6.587539327986601 1102 24-25 6.991853055438991 1102 26-27 8.581995218038987 1102 28-29 7.7662846820955345 1102 30-31 4.402421244043683 1102 32-33 4.474718414880758 1102 34-35 5.006612299068589 1102 36-37 5.274687045543118 1102 38-39 4.647590997986583 1102 40-41 6.4424463289222516 1102 42-43 7.1066720706003 1102 44-45 6.692746525831321 1102 46-47 5.724959664091344 1102 48-49 3.960123075688653 1102 50-51 5.192349112160954 1102 52-53 6.045107865051133 1102 54-55 5.497490631763387 1102 56-57 6.4511338700154575 1102 58-59 6.234127358181372 1102 60-61 6.24525645593258 1102 62-63 4.529073404250173 1102 64-65 6.3292220217549975 1102 66-67 4.084067107343518 1102 68-69 6.318601885698598 1102 70-71 5.489930635041617 1102 72-73 5.162958073023283 1102 74-75 4.447774061182603 1102 76-77 3.5369103467282343 1102 78-79 4.510030184825769 1102 80-81 4.6376270860270274 1102 82-83 4.036453237880426 1102 84-85 3.243556028694524 1102 86-87 3.320612355430473 1102 88-89 3.6007813650261156 1102 90-91 3.491762721877926 1102 92-93 3.361629227487576 1102 94-95 3.265830293023786 1102 96-97 3.4782056883764234 1102 98-99 3.226365061251407 1102 100 3.3372439871079678 1103 1 6.623414256923429 1103 2 10.88959621471043 1103 3 7.984007039264924 1103 4 11.9091576673156 1103 5 10.264229666902228 1103 6 9.067809481109808 1103 7 8.063753663979401 1103 8 8.031447839139897 1103 9 7.181284680650393 1103 10-11 7.448999752346001 1103 12-13 8.757956363486251 1103 14-15 8.649267481119939 1103 16-17 8.076292677414582 1103 18-19 7.331355764692528 1103 20-21 8.415123380242584 1103 22-23 6.956840077622726 1103 24-25 5.261421685317462 1103 26-27 6.024380183493502 1103 28-29 6.958368674118251 1103 30-31 6.155594212304383 1103 32-33 8.009486203065297 1103 34-35 7.296870421978436 1103 36-37 4.154721257710936 1103 38-39 6.549790256683266 1103 40-41 6.912054162723017 1103 42-43 7.625009923495124 1103 44-45 5.756101234825525 1103 46-47 6.4956570931393784 1103 48-49 5.745394503330431 1103 50-51 6.483712275790506 1103 52-53 6.224327425268836 1103 54-55 5.105923105784836 1103 56-57 5.981075964869898 1103 58-59 5.637996602783691 1103 60-61 5.142497043420018 1103 62-63 2.8467043366210234 1103 64-65 5.058048977308541 1103 66-67 4.2737283326803635 1103 68-69 5.391212023721799 1103 70-71 4.0576111675165905 1103 72-73 4.671504121363146 1103 74-75 3.3692327923087717 1103 76-77 3.746644734202661 1103 78-79 3.1887095999096005 1103 80-81 4.228507025602198 1103 82-83 3.646868327263782 1103 84-85 3.419436013901791 1103 86-87 2.954298871265209 1103 88-89 3.1932418119190835 1103 90-91 2.8382733148729606 1103 92-93 2.5079090609582053 1103 94-95 2.9338320952931696 1103 96-97 3.1595610786776582 1103 98-99 2.9009663061743396 1103 100 3.145305174590014 1104 1 6.248279832981257 1104 2 9.5727975620844 1104 3 7.405175836353489 1104 4 12.081388895785668 1104 5 9.295551048225779 1104 6 8.216829070435619 1104 7 4.002398779145956 1104 8 6.647558076417244 1104 9 6.6177281767820375 1104 10-11 7.291085226281819 1104 12-13 7.513535509651085 1104 14-15 7.39754704554618 1104 16-17 7.258658597067242 1104 18-19 6.854403091163075 1104 20-21 7.581942085946977 1104 22-23 6.5700748682267935 1104 24-25 5.970873677685237 1104 26-27 8.344425656561288 1104 28-29 7.29087779846984 1104 30-31 6.392833833731192 1104 32-33 7.704313212110481 1104 34-35 8.003358959172683 1104 36-37 6.584666893373427 1104 38-39 7.3633864949829615 1104 40-41 7.17147006758703 1104 42-43 7.282303718373424 1104 44-45 6.75543283236868 1104 46-47 6.676873929205669 1104 48-49 5.613859168922247 1104 50-51 5.875138469611748 1104 52-53 6.018137072125686 1104 54-55 4.8412587396630435 1104 56-57 6.054142209149351 1104 58-59 3.8606352021063053 1104 60-61 4.296318320258592 1104 62-63 3.9983086230770404 1104 64-65 2.8444299934329855 1104 66-67 3.2155983666299406 1104 68-69 4.294368966989673 1104 70-71 2.685665343781139 1104 72-73 2.642586346619897 1104 74-75 3.2291050754984276 1104 76-77 3.8262121041068085 1104 78-79 4.3863969856793865 1104 80-81 3.154875407081672 1104 82-83 3.0436113496766133 1104 84-85 3.0971308020303674 1104 86-87 0.7766533618687923 1104 88-89 0.21179362200075857 1104 90-91 2.176477824926831 1104 92-93 2.505392781394985 1104 94-95 2.5092972744688318 1104 96-97 2.8891200495970786 1104 98-99 2.5995555279059275 1104 100 0.8584479983206421 1105 1 5.730637800359389 1105 2 -2.8703012450942182 1105 3 0.42411036500825006 1105 4 8.221626467721595 1105 5 8.37064256435492 1105 6 8.00582710287765 1105 7 5.376305539788909 1105 8 6.000623307237561 1105 9 5.095782255036351 1105 10-11 4.943421855334005 1105 12-13 4.926873275138483 1105 14-15 5.790793594809582 1105 16-17 6.109633741890381 1105 18-19 6.136158814970361 1105 20-21 7.109767988458678 1105 22-23 2.1855880900381415 1105 24-25 4.0085641187020045 1105 26-27 6.945373239150616 1105 28-29 5.9998849462257375 1105 30-31 5.297540001292955 1105 32-33 7.2636178642650755 1105 34-35 6.272106960848145 1105 36-37 6.289865646368849 1105 38-39 4.196733563537876 1105 40-41 5.536151177798818 1105 42-43 5.625405439488485 1105 44-45 6.269877695934877 1105 46-47 5.479382688288311 1105 48-49 4.981937868938097 1105 50-51 6.045988308158645 1105 52-53 5.693235362406025 1105 54-55 5.37312129528944 1105 56-57 5.311886445838692 1105 58-59 1.9344229820181873 1105 60-61 4.417557777641573 1105 62-63 -0.858370026610821 1105 64-65 -0.4741193974391429 1105 66-67 0.8848556913499053 1105 68-69 2.8890745798020205 1105 70-71 2.895319327848213 1105 72-73 2.740045491678959 1105 74-75 2.778687543955062 1105 76-77 1.8232872779591673 1105 78-79 3.2241283770159477 1105 80-81 1.634066533874858 1105 82-83 0.9908081803752076 1105 84-85 1.4142661201315896 1105 86-87 -0.21244041741764264 1105 88-89 1.6358990084673373 1105 90-91 1.4251685812695705 1105 92-93 1.768040554772039 1105 94-95 1.099824730509198 1105 96-97 2.100880879802606 1105 98-99 2.0000068976613328 1105 100 2.156603651792917 1106 1 6.2391567769764045 1106 2 10.477022286207138 1106 3 6.973459180322859 1106 4 11.861711052059025 1106 5 9.944973970363701 1106 6 9.508901034744976 1106 7 6.753765593856489 1106 8 7.626285547979403 1106 9 6.985777956627622 1106 10-11 5.928761855428984 1106 12-13 7.984881899071667 1106 14-15 8.045547625265957 1106 16-17 7.863400102891607 1106 18-19 7.412870777614831 1106 20-21 8.380088636364135 1106 22-23 6.332115636947432 1106 24-25 5.283259601332549 1106 26-27 7.967887868048631 1106 28-29 6.5190449142784 1106 30-31 5.58185460367071 1106 32-33 7.595386625869384 1106 34-35 6.948938953646678 1106 36-37 6.181259185852415 1106 38-39 7.2858151255543575 1106 40-41 6.878251560566099 1106 42-43 6.400912277831335 1106 44-45 4.740415658433037 1106 46-47 6.224710017317069 1106 48-49 6.096875433499427 1106 50-51 6.849473373015304 1106 52-53 6.022435634714856 1106 54-55 5.59952251095984 1106 56-57 5.119907329673119 1106 58-59 5.233635191398552 1106 60-61 5.072659393829966 1106 62-63 5.149963273709563 1106 64-65 5.496904938040377 1106 66-67 3.3115492696788422 1106 68-69 3.4895822959611777 1106 70-71 3.175354640300746 1106 72-73 2.8936406225210796 1106 74-75 3.003994460402037 1106 76-77 3.712355385456835 1106 78-79 4.330387629304436 1106 80-81 3.8310688262364856 1106 82-83 2.2541042801075406 1106 84-85 2.86016651619099 1106 86-87 3.429053852564106 1106 88-89 3.5583810984924895 1106 90-91 3.3413206376074918 1106 92-93 3.2345677394821344 1106 94-95 1.7053306847612255 1106 96-97 1.7253875033603485 1106 98-99 2.147956174814354 1106 100 3.227095611057024 1107 1 6.268145883528781 1107 2 10.631009018541974 1107 3 6.252597280224915 1107 4 11.601303647080588 1107 5 9.507709577856982 1107 6 8.442559534768858 1107 7 4.0846183498251705 1107 8 6.6763300092025055 1107 9 6.0781875972358534 1107 10-11 4.956756184031239 1107 12-13 6.483150384140664 1107 14-15 6.5446332251387 1107 16-17 7.408494467735554 1107 18-19 6.575014924642556 1107 20-21 5.848251966250437 1107 22-23 6.1769844677639405 1107 24-25 4.989176512370836 1107 26-27 7.545041874670634 1107 28-29 6.870835414756282 1107 30-31 5.539976657241144 1107 32-33 5.302885738513577 1107 34-35 6.860523886050725 1107 36-37 6.786278455795493 1107 38-39 6.666613547138695 1107 40-41 7.953764106136354 1107 42-43 6.443024779035081 1107 44-45 4.390254575132481 1107 46-47 3.8218273025371587 1107 48-49 4.405609850888965 1107 50-51 5.145050017595473 1107 52-53 4.138423751288915 1107 54-55 5.480252521801631 1107 56-57 5.535623528584374 1107 58-59 3.2794753805250103 1107 60-61 3.12815594165367 1107 62-63 0.6719744205265172 1107 64-65 2.463079415972338 1107 66-67 4.429454026156819 1107 68-69 5.383785101802506 1107 70-71 4.537992307160884 1107 72-73 2.6049610606918208 1107 74-75 2.7412159096489965 1107 76-77 3.4231865428293915 1107 78-79 4.1277780992899515 1107 80-81 2.3940423034491936 1107 82-83 1.836315472478752 1107 84-85 2.616470415728749 1107 86-87 2.57048020617912 1107 88-89 2.853575014085841 1107 90-91 1.6282778358024608 1107 92-93 2.481624759253698 1107 94-95 2.484336110059484 1107 96-97 2.4603630708060216 1107 98-99 1.933723128481045 1107 100 0.19438424336488325 1108 1 4.252521553449359 1108 2 -3.1365185229305013 1108 3 0.09091148995552345 1108 4 6.532024472230756 1108 5 6.882177276496652 1108 6 6.678652504677309 1108 7 4.114840817115546 1108 8 4.928912631046405 1108 9 4.810858809055496 1108 10-11 3.0433589742773144 1108 12-13 2.256739669405505 1108 14-15 4.016515115911222 1108 16-17 4.543062619269204 1108 18-19 3.8912523994921493 1108 20-21 4.63030073515613 1108 22-23 3.9559970054088076 1108 24-25 2.717534921868907 1108 26-27 4.532545174572345 1108 28-29 5.941730544201736 1108 30-31 4.352788835405828 1108 32-33 4.475596353051142 1108 34-35 2.8889393531590457 1108 36-37 0.9693352176048435 1108 38-39 2.2134372672516562 1108 40-41 5.305691449468039 1108 42-43 3.1043896250721765 1108 44-45 3.576175283609345 1108 46-47 4.618923468814426 1108 48-49 4.752523338107736 1108 50-51 2.0849001890616883 1108 52-53 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-17.801909378890457 2212 34-35 -14.248742089757165 2212 36-37 -18.210243017682927 2212 38-39 -24.28498619167945 2212 40-41 -10.657462783055701 2212 42-43 -14.41160384458577 2212 44-45 -17.723547723325648 2212 46-47 -17.68804984168817 2212 48-49 -18.25718562433806 2212 50-51 -9.477267575866492 2212 52-53 -2.3465988007220098 2212 54-55 -24.186740622174863 2212 56-57 -24.12581950112506 2212 58-59 -13.118397983893598 2212 60-61 -6.664718679003325 2212 62-63 -3.6303053507561174 2212 64-65 -18.001044248038628 2212 66-67 -18.226459987339183 2212 68-69 -15.122683079198056 2212 70-71 -10.201835296423324 2212 72-73 -18.094819181981606 2212 74-75 -6.756312362948467 2212 76-77 -11.721782885488102 2212 78-79 -5.21056186244903 2212 80-81 -2.76677923788057 2212 82-83 -3.157927411208039 2212 84-85 -5.661144645822109 2212 86-87 -5.207209361641116 2212 88-89 -3.7444194169924856 2212 90-91 -4.373553957493517 2212 92-93 -5.728196107420587 2212 94-95 -4.051382050807508 2212 96-97 -5.827563513754253 2212 98-99 -3.874780206940393 2212 100 -4.188202797559551 2213 1 -21.31298372796322 2213 2 -17.14976146790922 2213 3 -20.02654081967714 2213 4 -19.073524952331756 2213 5 -21.25151340614563 2213 6 -22.218557017630747 2213 7 -10.819443940938843 2213 8 -10.655993457875857 2213 9 -4.358655228840277 2213 10-11 -18.02849485765272 2213 12-13 -16.715971239724272 2213 14-15 -24.06759335011432 2213 16-17 -18.342413028515487 2213 18-19 -19.014947281458692 2213 20-21 -18.154632308610253 2213 22-23 -20.321768554917938 2213 24-25 -7.916849138232045 2213 26-27 -23.78567964018408 2213 28-29 -24.704335985831374 2213 30-31 -20.0214051579727 2213 32-33 -23.939033948068204 2213 34-35 -18.460734282798917 2213 36-37 -24.355984031961608 2213 38-39 -11.987740428967587 2213 40-41 -17.69658478190406 2213 42-43 -23.897817532424273 2213 44-45 -17.731543387981116 2213 46-47 -17.570658600836623 2213 48-49 -6.564654913909031 2213 50-51 -23.997947044108738 2213 52-53 -18.419061017714554 2213 54-55 -18.31915587641215 2213 56-57 -18.16967543332845 2213 58-59 -18.678927467574507 2213 60-61 -19.941987224180203 2213 62-63 -18.235929719648453 2213 64-65 -18.33700476260522 2213 66-67 -9.15233926113768 2213 68-69 -23.376498933457043 2213 70-71 -18.07618040486953 2213 72-73 -9.906595129373308 2213 74-75 -4.693345656224718 2213 76-77 -3.494924716764338 2213 78-79 -5.155533238326321 2213 80-81 -3.6268085296213 2213 82-83 -3.5773509230457385 2213 84-85 -3.179578309626365 2213 86-87 -3.540708973588142 2213 88-89 -3.011223884191576 2213 90-91 -2.93945797488497 2213 92-93 -3.8544697974288518 2213 94-95 -3.982689059127697 2213 96-97 -3.726256547160194 2213 98-99 -3.9797393570626483 2213 100 -3.3810818168286794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 736.0 3 717.0 4 4132.0 5 15673.0 6 21139.0 7 24611.0 8 22604.0 9 22137.0 10 25227.0 11 30237.0 12 37975.0 13 50865.0 14 38224.0 15 42205.0 16 48840.0 17 53400.0 18 53567.0 19 53917.0 20 53189.0 21 50860.0 22 53561.0 23 60212.0 24 66101.0 25 73626.0 26 81535.0 27 88027.0 28 96957.0 29 103120.0 30 109767.0 31 121917.0 32 140127.0 33 170410.0 34 208554.0 35 246397.0 36 272984.0 37 234826.0 38 72035.0 39 103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.50591270915502 21.61033421017864 14.085036861388767 23.798716219277573 2 14.948262610886642 21.504772442645162 40.70630939336216 22.84065555310604 3 17.594538877028132 27.184003630364767 30.296996487202737 24.92446100540436 4 11.874505878683697 18.54550630661996 40.15909799740337 29.420889817292977 5 12.95647995743894 38.12294379072228 36.30742857462385 12.613147677214934 6 29.412742539717513 39.21562749761261 19.121634536962816 12.24999542570706 7 25.453887230998962 32.32225859460088 24.621846745658598 17.60200742874156 8 23.427094986947953 36.57202483942622 22.238513155121336 17.762367018504495 9 24.195547589429424 17.19787797856577 22.62922290142365 35.97735153058115 10-11 22.657167008451452 27.595969701946593 30.078950778470727 19.667912511131235 12-13 23.586985685893612 25.268071181238188 29.792542536926803 21.352400595941393 14-15 21.017639926155354 26.96679633910353 27.06318075414863 24.95238298059249 16-17 20.684784612865087 30.480363147866857 27.724572911493205 21.11027932777485 18-19 20.612602832196163 29.633113332672867 29.26875803531035 20.48552579982062 20-21 22.018964820187893 28.912193604410113 28.3181703510877 20.750671224314296 22-23 21.24201491636841 28.92063806443419 28.74268304031925 21.094663978878145 24-25 21.4031760244899 28.798184046966195 28.367602411865988 21.431037516677915 26-27 21.283407672536992 28.738148330581385 28.49408031512018 21.484363681761444 28-29 21.11286086876124 28.6799907054674 28.860536438562406 21.346611987208952 30-31 21.61490831500123 29.053677866694088 28.247646819028173 21.08376699927651 32-33 20.92348533194861 28.83191407205208 28.77762037779715 21.466980218202163 34-35 21.076416635065577 28.386623329831206 28.63643112270711 21.900528912396105 36-37 21.60241589846329 28.865804342778663 28.213105629004094 21.318674129753955 38-39 21.276796689924893 28.680030124915163 28.438832564158044 21.6043406210019 40-41 21.481383284486704 28.686504882654273 28.194499680254005 21.637612152605016 42-43 21.191052017861125 28.766675351452246 28.60762739416638 21.43464523652025 44-45 21.543581974082908 28.404860466615588 28.524036955019167 21.527520604282333 46-47 21.420282331523737 28.78002485156332 28.55179278147736 21.247900035435592 48-49 21.291127021156786 28.527197402388456 28.63047659443766 21.551198982017098 50-51 21.58316635900579 28.48825189849388 28.41883338975817 21.50974835274216 52-53 21.150523041959637 28.5008743124357 28.57446963530574 21.77413301029893 54-55 21.481206634124987 28.64873269178044 28.327956246765844 21.542104427328727 56-57 21.575927543883854 28.531433249509362 28.30816121164509 21.5844779949617 58-59 21.223868515968427 28.14644139831416 28.67138919079897 21.958300894918445 60-61 21.579738428078706 28.561427661303906 28.279589774383236 21.579244136234156 62-63 21.254403699143474 28.607196912940854 28.52232594937854 21.616073438537136 64-65 21.30657552937183 28.117993554115706 28.57470866594148 22.000722250570988 66-67 21.34762742107792 28.331321096145796 28.346520930748547 21.974530552027737 68-69 21.74096012023859 28.31440658024117 28.30877826492739 21.63585503459285 70-71 21.776282754611202 28.341865459441664 28.12449796014227 21.757353825804866 72-73 21.46863020693391 28.44389398138583 28.512886223639057 21.574589588041203 74-75 21.685531081312686 28.140655917892698 28.160043229278063 22.01376977151655 76-77 21.67745054093276 28.476685927514332 28.1636217349811 21.6822417965718 78-79 21.683947871401266 28.24243112516749 28.328515737717098 21.745105265714145 80-81 21.74606272657082 28.16391887508934 28.225037385082132 21.86498101325771 82-83 21.328744245199214 28.348335440579724 28.598797400209108 21.724122914011957 84-85 21.521851371417846 28.015533348125643 28.23581787252442 22.226797407932093 86-87 21.632502763483693 28.310059433187508 28.238158619997943 21.819279183330856 88-89 21.455926173035657 27.973246418767438 28.47823766888406 22.092589739312846 90-91 21.691773831250508 27.976074752674446 28.18882337799846 22.143328038076586 92-93 21.473979408158396 28.156179004842087 28.396021058357633 21.973820528641884 94-95 21.372568573271757 27.85591427646053 28.407130331797468 22.364386818470237 96-97 21.497798616273087 28.000104474840974 28.38636137423947 22.11573553464647 98-99 21.427128524802384 27.858233722610137 28.384473066148853 22.33016468643863 100 21.61180442302592 27.66683689880311 28.18673854036759 22.534620137803387 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 300.0 1 365.0 2 356.0 3 282.0 4 342.5 5 505.0 6 736.5 7 1132.0 8 1784.0 9 2631.5 10 3539.5 11 4447.0 12 5491.5 13 6478.0 14 7322.5 15 8091.0 16 8864.5 17 9711.5 18 10417.5 19 11146.5 20 12255.0 21 13306.0 22 14444.0 23 16227.5 24 18614.0 25 21815.0 26 26403.5 27 32583.0 28 40055.5 29 48592.5 30 57346.5 31 66475.5 32 75073.0 33 83096.5 34 90898.5 35 97228.5 36 102387.5 37 106757.0 38 109845.0 39 111485.5 40 111758.0 41 110336.0 42 107744.0 43 103950.5 44 98987.5 45 94111.0 46 89225.5 47 84517.5 48 80525.0 49 77172.0 50 74182.5 51 69368.5 52 63077.5 53 57649.5 54 53096.5 55 48846.5 56 44606.5 57 40388.5 58 36074.5 59 31733.0 60 27541.0 61 23493.0 62 19609.0 63 15890.0 64 12808.5 65 10367.5 66 8250.5 67 6464.5 68 5005.0 69 3804.0 70 2886.5 71 2168.5 72 1635.0 73 1267.5 74 983.0 75 732.0 76 480.0 77 303.5 78 220.0 79 167.0 80 120.0 81 87.0 82 55.5 83 38.5 84 26.0 85 17.0 86 14.5 87 14.0 88 8.0 89 6.5 90 5.0 91 4.0 92 2.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.860091197587522 2 24.731223912599624 3 6.30440685434276 4 22.612693710678144 5 12.16682324661447 6 9.886322256266764 7 8.329024168974437 8 9.125231449485954 9 5.582080986095841 10-11 6.869708410483162 12-13 10.109808266158314 14-15 7.7508652825420254 16-17 8.10292459535368 18-19 6.869813654660177 20-21 8.1511790505151 22-23 5.946278460656569 24-25 5.50188492321034 26-27 9.798390746370654 28-29 6.470078729660686 30-31 4.476525987944631 32-33 8.829477771377373 34-35 7.257691069049302 36-37 7.22788942625786 38-39 6.2086872753475335 40-41 8.304484735033752 42-43 6.555028321208035 44-45 6.406634031616754 46-47 5.999075956125807 48-49 4.058706605194713 50-51 6.584058173368031 52-53 4.6640711113855255 54-55 5.121269357035257 56-57 5.018551040268527 58-59 3.5232593139342585 60-61 4.186402873306358 62-63 3.0694815040375176 64-65 4.5068012295326385 66-67 2.601916706951799 68-69 5.258121868547216 70-71 2.6079507064339977 72-73 1.6847838670499427 74-75 0.9297270597513291 76-77 1.1535112614777545 78-79 1.7667690809446999 80-81 2.2208626233724864 82-83 0.9167820259784727 84-85 1.3415826058037252 86-87 0.48999934748610247 88-89 0.7283598677291183 90-91 0.30389256113108026 92-93 0.36174177709704286 94-95 0.6204670455924791 96-97 0.6067677618843479 98-99 0.4901572137516252 100 0.5321847217729855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2850514.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.80911271411384 #Duplication Level Percentage of deduplicated Percentage of total 1 96.92484517823495 92.86283416479546 2 2.5373855978234294 4.862093254820682 3 0.34565275249011623 0.9935005056980772 4 0.09233075948497971 0.3538451256990463 5 0.03791841884491707 0.18164650325268192 6 0.02080467141681027 0.11959662652519666 7 0.013195104987702405 0.0884947910728942 8 0.005138706686872464 0.03938679425338676 9 0.006239351117689243 0.053800802504785875 >10 0.015154531878192339 0.20529957548222535 >50 5.969440143583184E-4 0.042042321194841976 >100 6.646010763879644E-4 0.13182833382386822 >500 7.33819435927985E-5 0.06563120087689768 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.016278467672848E-5 0.0 5 0.0 0.0 0.0 7.016278467672848E-5 0.0 6 0.0 0.0 0.0 7.016278467672848E-5 0.0 7 0.0 0.0 0.0 7.016278467672848E-5 0.0 8 0.0 0.0 0.0 1.0524417701509271E-4 0.0 9 0.0 0.0 0.0 1.754069616918212E-4 0.0 10-11 3.508139233836424E-5 0.0 0.0 1.929476578610033E-4 0.0 12-13 3.508139233836424E-5 0.0 0.0 2.1048835403018542E-4 0.0 14-15 3.508139233836424E-5 0.0 0.0 2.1048835403018542E-4 0.0 16-17 3.508139233836424E-5 0.0 0.0 2.1048835403018542E-4 0.0 18-19 7.016278467672848E-5 0.0 0.0 2.1048835403018542E-4 0.0 20-21 1.0524417701509271E-4 0.0 0.0 2.1048835403018542E-4 0.0 22-23 1.0524417701509271E-4 0.0 0.0 2.1048835403018542E-4 0.0 24-25 1.0524417701509271E-4 3.508139233836424E-5 0.0 2.8065113870691394E-4 0.0 26-27 1.0524417701509271E-4 3.508139233836424E-5 0.0 4.560581003987351E-4 0.0 28-29 1.2278487318427485E-4 3.508139233836424E-5 0.0 7.542499352748311E-4 0.0 30-31 1.4032556935345697E-4 3.508139233836424E-5 0.0 0.0020698021479634903 0.0 32-33 1.4032556935345697E-4 3.508139233836424E-5 0.0 0.005420075116277274 0.0 34-35 1.4032556935345697E-4 5.262208850754636E-5 0.0 0.011559318775491018 0.0 36-37 1.4032556935345697E-4 7.016278467672848E-5 0.0 0.02125932375704873 0.0 38-39 1.4032556935345697E-4 1.0524417701509271E-4 0.0 0.03660743290508308 0.0 40-41 1.4032556935345697E-4 1.0524417701509271E-4 0.0 0.05442878021297212 0.0 42-43 1.4032556935345697E-4 1.0524417701509271E-4 0.0 0.07333765068335044 3.508139233836424E-5 44-45 1.5786626552263908E-4 1.0524417701509271E-4 0.0 0.09654399171517838 5.262208850754636E-5 46-47 1.754069616918212E-4 1.0524417701509271E-4 0.0 0.12271471039959811 7.016278467672848E-5 48-49 1.754069616918212E-4 1.0524417701509271E-4 0.0 0.15244619040636181 7.016278467672848E-5 50-51 1.754069616918212E-4 1.0524417701509271E-4 0.0 0.18417730977641225 7.016278467672848E-5 52-53 1.754069616918212E-4 1.0524417701509271E-4 0.0 0.21931132420328403 7.016278467672848E-5 54-55 1.754069616918212E-4 1.0524417701509271E-4 0.0 0.2611458845667834 7.016278467672848E-5 56-57 1.754069616918212E-4 1.0524417701509271E-4 0.0 0.3107334326370612 7.016278467672848E-5 58-59 1.754069616918212E-4 1.2278487318427485E-4 0.0 0.36168915500853527 7.016278467672848E-5 60-61 1.754069616918212E-4 1.4032556935345697E-4 0.0 0.41767905718056464 7.016278467672848E-5 62-63 1.754069616918212E-4 1.4032556935345697E-4 0.0 0.4799485285811611 7.016278467672848E-5 64-65 1.929476578610033E-4 1.4032556935345697E-4 0.0 0.5494798481957991 7.016278467672848E-5 66-67 2.1048835403018542E-4 1.4032556935345697E-4 0.0 0.6314124400020488 7.016278467672848E-5 68-69 2.4556974636854965E-4 1.4032556935345697E-4 0.0 0.7250797575454813 7.016278467672848E-5 70-71 2.4556974636854965E-4 1.5786626552263908E-4 0.0 0.8309553996226646 7.016278467672848E-5 72-73 2.4556974636854965E-4 1.754069616918212E-4 0.0 0.9466187501622514 7.016278467672848E-5 74-75 2.8065113870691394E-4 1.754069616918212E-4 0.0 1.068421344361052 7.016278467672848E-5 76-77 2.8065113870691394E-4 1.754069616918212E-4 0.0 1.2052563151768418 7.016278467672848E-5 78-79 2.9819183487609605E-4 1.754069616918212E-4 0.0 1.3580884008989256 7.016278467672848E-5 80-81 3.1573253104527816E-4 1.754069616918212E-4 0.0 1.5308642581653693 7.016278467672848E-5 82-83 3.1573253104527816E-4 1.754069616918212E-4 0.0 1.7184970850871106 7.016278467672848E-5 84-85 3.1573253104527816E-4 1.754069616918212E-4 0.0 1.9116727719983133 7.016278467672848E-5 86-87 3.1573253104527816E-4 1.929476578610033E-4 0.0 2.127300550006069 7.016278467672848E-5 88 3.1573253104527816E-4 2.1048835403018542E-4 0.0 2.306741871816802 7.016278467672848E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6735 0.0 19.343275 1 TACATGG 6985 0.0 18.124973 2 GTATCAA 4395 0.0 17.92312 1 GTGTAAG 1090 0.0 17.233137 1 TCAACGC 4645 0.0 17.164196 4 ATCAACG 4695 0.0 16.863905 3 ACATGGG 7255 0.0 16.824678 3 GTATTAG 1170 0.0 16.5727 1 CAACGCA 4610 0.0 16.110672 5 CATGGGG 3655 0.0 15.94629 4 TATACGG 190 0.002724197 15.740769 5 TATCAAC 4875 0.0 15.656454 2 AACGCAG 4760 0.0 15.646558 6 TAGCGCA 215 0.0032285356 15.344544 4 GTATATA 2025 0.0 15.2606945 1 TATTACG 285 6.424892E-4 14.878209 2 CGCAGAG 5055 0.0 14.840688 8 GTCTTAC 870 0.0 14.6261215 1 GTATAAG 1085 0.0 14.520205 1 GTATAGC 670 8.913048E-11 14.470192 1 >>END_MODULE