FastQCFastQC Report
Wed 25 May 2016
SRR1295120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850514
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67400.024.0796851
ATCAACG73450.021.2464563
TCAACGC73850.020.9404324
CAACGCA74450.020.8348065
AACGCAG76600.020.3509316
TATCAAC77250.020.2156662
ACCGTAC1550.001041104618.1745688
GTACCGT1300.00808702118.0593326
ACGCAGA86400.017.9870017
CGCAGAG86700.017.9247638
GTACATG90600.016.5947931
GTACTAG6050.016.4576451
TACATGG92700.016.1489282
GGTATCA50700.016.0056291
ACATGGG90800.015.8925213
GTATAAG11750.015.6767841
GAGTACT34350.015.64977312-13
GCAGAGT100950.015.3474749
TATACTG17000.014.9308695
CATGGGG40650.014.9165854
Produced by FastQC (version 0.11.5)