##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295120_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2850514 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.437860680564977 33.0 33.0 33.0 27.0 33.0 2 30.081421105105957 33.0 33.0 33.0 27.0 33.0 3 30.92049574217141 33.0 33.0 33.0 27.0 33.0 4 35.2296168340166 37.0 37.0 37.0 33.0 37.0 5 35.529501696886946 37.0 37.0 37.0 33.0 37.0 6 35.306264764881 37.0 37.0 37.0 33.0 37.0 7 35.20822209608513 37.0 37.0 37.0 33.0 37.0 8 35.19380680115937 37.0 37.0 37.0 33.0 37.0 9 35.162839403700524 37.0 37.0 37.0 33.0 37.0 10-11 35.13846344904813 37.0 37.0 37.0 33.0 37.0 12-13 35.05912775730974 37.0 37.0 37.0 33.0 37.0 14-15 36.527114232731364 40.0 37.0 40.0 33.0 40.0 16-17 36.52639453796753 40.0 37.0 40.0 33.0 40.0 18-19 36.55147176965277 40.0 37.0 40.0 33.0 40.0 20-21 36.55195080606515 40.0 37.0 40.0 33.0 40.0 22-23 36.502882638008444 40.0 37.0 40.0 33.0 40.0 24-25 36.475290596713435 40.0 37.0 40.0 33.0 40.0 26-27 36.44930879132676 40.0 37.0 40.0 33.0 40.0 28-29 36.3999968075933 38.5 37.0 40.0 33.0 40.0 30-31 36.31623138844433 37.0 37.0 40.0 33.0 40.0 32-33 36.27839786087702 37.0 37.0 40.0 30.0 40.0 34-35 36.17542187128356 37.0 37.0 40.0 27.0 40.0 36-37 36.17329418483824 37.0 37.0 40.0 27.0 40.0 38-39 36.115284646909295 37.0 37.0 40.0 27.0 40.0 40-41 36.05606006495671 37.0 37.0 40.0 27.0 40.0 42-43 35.96989121961864 37.0 37.0 40.0 27.0 40.0 44-45 35.863839644358876 37.0 37.0 40.0 27.0 40.0 46-47 35.763055890972645 37.0 37.0 40.0 27.0 40.0 48-49 35.66189185529346 37.0 37.0 40.0 27.0 40.0 50-51 35.5605606567798 37.0 37.0 40.0 27.0 40.0 52-53 35.46246203316314 37.0 37.0 40.0 27.0 40.0 54-55 35.345750099806565 37.0 33.0 40.0 27.0 40.0 56-57 35.2286484121811 37.0 33.0 40.0 27.0 40.0 58-59 35.101678328890856 37.0 33.0 40.0 27.0 40.0 60-61 34.944032023698185 37.0 33.0 40.0 27.0 40.0 62-63 34.76720601968627 37.0 33.0 40.0 27.0 40.0 64-65 34.586927129633466 37.0 33.0 40.0 27.0 40.0 66-67 34.36829831391812 37.0 33.0 40.0 27.0 40.0 68-69 34.151515305660666 37.0 33.0 38.5 27.0 40.0 70-71 33.92835362324128 37.0 33.0 37.0 27.0 40.0 72-73 33.71784264171304 37.0 33.0 37.0 27.0 40.0 74-75 33.47638496074743 37.0 33.0 37.0 24.5 40.0 76-77 33.2438707545376 37.0 33.0 37.0 22.0 38.5 78-79 33.002045595987255 37.0 33.0 37.0 22.0 37.0 80-81 32.78530679028414 37.0 33.0 37.0 22.0 37.0 82-83 32.56806368956616 37.0 33.0 37.0 22.0 37.0 84-85 32.40035832134134 35.0 33.0 37.0 22.0 37.0 86-87 32.4188377955695 33.0 33.0 37.0 22.0 37.0 88-89 32.43044464963161 33.0 33.0 37.0 22.0 37.0 90-91 32.439974860674255 33.0 33.0 37.0 22.0 37.0 92-93 32.408066931086815 33.0 33.0 37.0 22.0 37.0 94-95 32.43717869829792 33.0 33.0 37.0 22.0 37.0 96-97 32.40157266373714 33.0 33.0 37.0 22.0 37.0 98-99 32.326943316187894 33.0 33.0 37.0 22.0 37.0 100 31.789297649476552 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5949193289962658 1101 2 4.162024190669147 1101 3 2.3940436208767544 1101 4 1.113138700805223 1101 5 0.5106689265728264 1101 6 0.5747922682174078 1101 7 0.5512019555485352 1101 8 0.5286019252841427 1101 9 0.6258275762699768 1101 10-11 0.6375664597133763 1101 12-13 0.6574674844332691 1101 14-15 0.9581214220516117 1101 16-17 0.9634251958513644 1101 18-19 0.9935236412953401 1101 20-21 0.9565551273236608 1101 22-23 0.897658547017187 1101 24-25 0.9069991722984625 1101 26-27 0.9461843643341226 1101 28-29 0.8834899034455006 1101 30-31 0.7352057056699337 1101 32-33 0.9823149773403372 1101 34-35 1.1069409613169583 1101 36-37 1.0621842425174108 1101 38-39 0.9646902060948577 1101 40-41 1.0080674035604176 1101 42-43 1.0939908823930153 1101 44-45 1.074039097207546 1101 46-47 0.9768791970151156 1101 48-49 0.990665722345426 1101 50-51 0.9664851570403314 1101 52-53 0.9495096179789186 1101 54-55 0.9538186009169252 1101 56-57 1.0216156125329263 1101 58-59 0.9435368082448221 1101 60-61 0.9667631589670478 1101 62-63 0.90148715976283 1101 64-65 0.9079917942862181 1101 66-67 0.9034138428635856 1101 68-69 0.9103920616793744 1101 70-71 0.8592731065101091 1101 72-73 0.9219560286637716 1101 74-75 0.9066346229939555 1101 76-77 0.9132861912305401 1101 78-79 0.9763033775266052 1101 80-81 0.8927626473509207 1101 82-83 0.8525310693358819 1101 84-85 0.85809854718471 1101 86-87 0.9213951976217203 1101 88-89 0.8791776200672246 1101 90-91 0.8565834392383422 1101 92-93 0.9221074191805059 1101 94-95 0.9850513067075113 1101 96-97 0.867644903159885 1101 98-99 0.888777423681077 1101 100 0.9175693038942754 1102 1 0.4454433998920102 1102 2 4.026966902129697 1102 3 2.2636628541115975 1102 4 0.9655441200888006 1102 5 0.44767043383951943 1102 6 0.40097026048269413 1102 7 0.4384655422181254 1102 8 0.416463324732085 1102 9 0.44949434461926785 1102 10-11 0.49531284204376647 1102 12-13 0.5415060663972255 1102 14-15 0.7255549867153661 1102 16-17 0.6759027922186078 1102 18-19 0.7483695120185274 1102 20-21 0.6398407743372516 1102 22-23 0.7170477953442287 1102 24-25 0.7167233574382124 1102 26-27 0.7346883447547938 1102 28-29 0.7420359131740142 1102 30-31 0.6448209726962304 1102 32-33 0.7408687842021422 1102 34-35 0.8560812254202474 1102 36-37 0.7149790080989007 1102 38-39 0.6898190602547132 1102 40-41 0.6404891609273022 1102 42-43 0.6947223111257514 1102 44-45 0.6859409022646616 1102 46-47 0.6766197988688276 1102 48-49 0.6443334469607436 1102 50-51 0.6216517846847012 1102 52-53 0.6236981007461537 1102 54-55 0.6921970750855806 1102 56-57 0.5629455836974557 1102 58-59 0.5745582683585155 1102 60-61 0.6213770277351145 1102 62-63 0.6623283861979345 1102 64-65 0.6426247873978781 1102 66-67 0.5885846599103601 1102 68-69 0.566875923786796 1102 70-71 0.6574286949961134 1102 72-73 0.6020251078948533 1102 74-75 0.6430513354959544 1102 76-77 0.6126780465790489 1102 78-79 0.6126977291298914 1102 80-81 0.6066898804884744 1102 82-83 0.6000478179102942 1102 84-85 0.6517720050006304 1102 86-87 0.6316751647668042 1102 88-89 0.5901374127440633 1102 90-91 0.6376506687442429 1102 92-93 0.6067647438412251 1102 94-95 0.5510381063293437 1102 96-97 0.5337365281938204 1102 98-99 0.6150435531001079 1102 100 0.5810213685448886 1103 1 0.5074742821072391 1103 2 4.009869647039139 1103 3 2.3024463659259453 1103 4 1.0315527322540774 1103 5 0.42215842188470276 1103 6 0.3959191842794709 1103 7 0.4439802801946655 1103 8 0.3199943958245086 1103 9 0.41143906153362053 1103 10-11 0.48943473370444224 1103 12-13 0.4391497539308489 1103 14-15 0.6400657352563286 1103 16-17 0.6347518178344842 1103 18-19 0.6123217832271308 1103 20-21 0.5909868045830038 1103 22-23 0.5780975676599169 1103 24-25 0.524518589161957 1103 26-27 0.6002569345563771 1103 28-29 0.6398214570054321 1103 30-31 0.6113413984159095 1103 32-33 0.6893152295809202 1103 34-35 0.7589773159797133 1103 36-37 0.709612276833063 1103 38-39 0.5623128165936038 1103 40-41 0.6646685172846034 1103 42-43 0.7509873300372831 1103 44-45 0.7599959905211051 1103 46-47 0.6977627360520771 1103 48-49 0.704732981751448 1103 50-51 0.6309525482088674 1103 52-53 0.5786725150284511 1103 54-55 0.6102778903208232 1103 56-57 0.5369045866321969 1103 58-59 0.595047376969255 1103 60-61 0.4687492241388327 1103 62-63 0.6254510063849281 1103 64-65 0.635527458899162 1103 66-67 0.6228210325793597 1103 68-69 0.6079471412034891 1103 70-71 0.616879419475076 1103 72-73 0.6046303533637811 1103 74-75 0.6227725809267852 1103 76-77 0.5476895597552129 1103 78-79 0.4903358098677515 1103 80-81 0.5920775906143589 1103 82-83 0.6354110057447002 1103 84-85 0.6377859660411431 1103 86-87 0.6482427403454523 1103 88-89 0.6240322390960813 1103 90-91 0.5862584632617214 1103 92-93 0.6886923110235728 1103 94-95 0.5270968304718124 1103 96-97 0.5460270867941617 1103 98-99 0.5431954214765042 1103 100 0.6013777472769988 1104 1 0.353643260035188 1104 2 3.6193464947755913 1104 3 2.1556447716459353 1104 4 0.9550568690554542 1104 5 0.30999430090074753 1104 6 0.2937093671924842 1104 7 0.2635146308366032 1104 8 0.2336957319462556 1104 9 0.2298937005862456 1104 10-11 0.3702737360175803 1104 12-13 0.3308879961559654 1104 14-15 0.44517121284108185 1104 16-17 0.39986893544759994 1104 18-19 0.39426987348286957 1104 20-21 0.4196602251318211 1104 22-23 0.32421705587641725 1104 24-25 0.33680851407744683 1104 26-27 0.34679210219046297 1104 28-29 0.4382427593222644 1104 30-31 0.3467586109495997 1104 32-33 0.5240314483821109 1104 34-35 0.556237679683889 1104 36-37 0.4401368798303409 1104 38-39 0.3917005527095654 1104 40-41 0.39842703080093855 1104 42-43 0.3906527510554767 1104 44-45 0.3848147995048805 1104 46-47 0.4282951754226687 1104 48-49 0.31780873416384026 1104 50-51 0.32736828147639585 1104 52-53 0.3217757027847341 1104 54-55 0.33570588690979974 1104 56-57 0.3381925950520781 1104 58-59 0.38024806448040493 1104 60-61 0.37653595703825715 1104 62-63 0.4059311831735144 1104 64-65 0.3520447957699844 1104 66-67 0.3733024672063223 1104 68-69 0.3275352312823969 1104 70-71 0.3480604694365823 1104 72-73 0.32730105167707535 1104 74-75 0.34554223856970623 1104 76-77 0.33302542470596563 1104 78-79 0.3370688559745645 1104 80-81 0.3645624563672385 1104 82-83 0.4109062444446536 1104 84-85 0.41250691077823376 1104 86-87 0.4108320247680268 1104 88-89 0.3187591836820758 1104 90-91 0.324291668185694 1104 92-93 0.4567044160100835 1104 94-95 0.3772984261985428 1104 96-97 0.30943969274593996 1104 98-99 0.36258449781323066 1104 100 0.283785511153809 1105 1 0.41819971935608535 1105 2 3.742719133873752 1105 3 2.1777073259505464 1105 4 0.9245966703730559 1105 5 0.28902036721373037 1105 6 0.3192147724444325 1105 7 0.22438819994111014 1105 8 0.19991437170670423 1105 9 0.2938698760016152 1105 10-11 0.2908587543187977 1105 12-13 0.28269222599004706 1105 14-15 0.41452826728593806 1105 16-17 0.37497407998735355 1105 18-19 0.37459031336510407 1105 20-21 0.2913584898175898 1105 22-23 0.2844887118564827 1105 24-25 0.347073380886016 1105 26-27 0.40071266848347165 1105 28-29 0.34410104578144285 1105 30-31 0.3202071175371515 1105 32-33 0.3787863868284589 1105 34-35 0.4731336018851877 1105 36-37 0.29097395062927944 1105 38-39 0.3366693861065997 1105 40-41 0.35836856582060506 1105 42-43 0.4850834049257955 1105 44-45 0.437331456757164 1105 46-47 0.3820371229195345 1105 48-49 0.35857501783645773 1105 50-51 0.25777140054995584 1105 52-53 0.2763905920386307 1105 54-55 0.28039076170716015 1105 56-57 0.31666006926299417 1105 58-59 0.2976741654001316 1105 60-61 0.2933017409357248 1105 62-63 0.3542855525911577 1105 64-65 0.3009467799604266 1105 66-67 0.3579333175220114 1105 68-69 0.27404324395672575 1105 70-71 0.24517289110701057 1105 72-73 0.3659005582505017 1105 74-75 0.3050917314796777 1105 76-77 0.33603550352144396 1105 78-79 0.3218781473462755 1105 80-81 0.30799100899898946 1105 82-83 0.35154531626677965 1105 84-85 0.3181974331048423 1105 86-87 0.3767885729689908 1105 88-89 0.354298656455839 1105 90-91 0.2554098782757066 1105 92-93 0.26338633761798036 1105 94-95 0.2928836027134949 1105 96-97 0.3171486067178293 1105 98-99 0.31000733382239787 1105 100 0.3099791962029421 1106 1 0.44581915460044286 1106 2 3.9286271198762286 1106 3 2.262339078993886 1106 4 0.9599503624340073 1106 5 0.37441825028249553 1106 6 0.2949467131546939 1106 7 0.21355017657527497 1106 8 0.1635486134177242 1106 9 0.28334695608855043 1106 10-11 0.21691804115892666 1106 12-13 0.19297417025462948 1106 14-15 0.27970254698013974 1106 16-17 0.2985765146056636 1106 18-19 0.2660471023743014 1106 20-21 0.27843289992564024 1106 22-23 0.22405565400532623 1106 24-25 0.24170940281097586 1106 26-27 0.2662336948477062 1106 28-29 0.17142196688646294 1106 30-31 0.17541116326272999 1106 32-33 0.31151026179090024 1106 34-35 0.3818254252704705 1106 36-37 0.2260698488932391 1106 38-39 0.26210343643766976 1106 40-41 0.2745855571899014 1106 42-43 0.25350648094129014 1106 44-45 0.1890787295166021 1106 46-47 0.16351403489881022 1106 48-49 0.12277462246356663 1106 50-51 0.036748030852919555 1106 52-53 0.03542880899894385 1106 54-55 0.08128406006498068 1106 56-57 0.16605206059107047 1106 58-59 0.12553277513939065 1106 60-61 0.1100369601145843 1106 62-63 0.19009973712343253 1106 64-65 0.12543862274009143 1106 66-67 0.05808888355038988 1106 68-69 0.057414061543397565 1106 70-71 0.013022981291406666 1106 72-73 -0.033896679099783 1106 74-75 -0.08967991829408106 1106 76-77 0.02817909798078233 1106 78-79 0.034896865736435245 1106 80-81 0.052600940033258325 1106 82-83 0.07400430625121146 1106 84-85 0.08846479688356368 1106 86-87 0.12882821330815375 1106 88-89 0.14891105915510394 1106 90-91 0.05658373337008982 1106 92-93 0.09650181830908622 1106 94-95 0.09395897260019126 1106 96-97 0.05972912732551805 1106 98-99 0.09075703004381808 1106 100 0.15322647335453965 1107 1 0.46133972822573455 1107 2 3.6350142283792763 1107 3 2.1927219267555778 1107 4 0.9667827589561355 1107 5 0.2802533013718147 1107 6 0.2828974887520488 1107 7 0.13228799287160342 1107 8 0.16201838861734075 1107 9 0.17653601583748468 1107 10-11 0.1882600835573669 1107 12-13 0.11317354462846652 1107 14-15 0.07195373832537655 1107 16-17 0.009309567517668427 1107 18-19 0.22457292651448313 1107 20-21 0.1912663507795429 1107 22-23 0.13309537401199378 1107 24-25 0.22163687180906777 1107 26-27 0.10930663168082333 1107 28-29 0.053499432574732 1107 30-31 0.029738569576359453 1107 32-33 0.19846492066101717 1107 34-35 0.2775836443764206 1107 36-37 0.2087295751140843 1107 38-39 0.13624268338757872 1107 40-41 0.11978338672246736 1107 42-43 0.18689588164578197 1107 44-45 0.30586669502130803 1107 46-47 0.13978556726002012 1107 48-49 0.11796870700393214 1107 50-51 0.0927007612184525 1107 52-53 0.06989298918534814 1107 54-55 0.1109795955274464 1107 56-57 0.21743284151640552 1107 58-59 0.24185366613932757 1107 60-61 0.18794499518860164 1107 62-63 0.2311096414630711 1107 64-65 0.159693641400807 1107 66-67 0.1537488983634674 1107 68-69 0.12024146358215404 1107 70-71 0.149932677216988 1107 72-73 0.1912443673406301 1107 74-75 0.19087879563208077 1107 76-77 0.1488813098709585 1107 78-79 0.09061669240089287 1107 80-81 0.1882860644686275 1107 82-83 0.17096737612293111 1107 84-85 0.1485614647825173 1107 86-87 0.14768613045458778 1107 88-89 0.2754301808087831 1107 90-91 0.22083750387072598 1107 92-93 0.22247371801742588 1107 94-95 0.15741447356879945 1107 96-97 0.00845667727483601 1107 98-99 0.09852427952137788 1107 100 0.10708138605987116 1108 1 0.37886997961944147 1108 2 3.6319648850114596 1108 3 2.1127183351448764 1108 4 0.8763736359973109 1108 5 0.2652355252664833 1108 6 0.13226547490071994 1108 7 0.07341162143400481 1108 8 0.10670663160992433 1108 9 0.09968494018919927 1108 10-11 0.03611328035596273 1108 12-13 0.010224988198103802 1108 14-15 -0.011236018079721077 1108 16-17 -0.0663542255001488 1108 18-19 0.009242752851520208 1108 20-21 -0.02021353437332607 1108 22-23 -0.08065994424058687 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0.45253783596543684 2210 60-61 0.49517100110494994 2210 62-63 0.4555330287332211 2210 64-65 0.19332237079908055 2210 66-67 0.29939802103401547 2210 68-69 0.5229680784834798 2210 70-71 0.48832057504233717 2210 72-73 0.476911365238081 2210 74-75 0.07567476243813331 2210 76-77 0.3281239970276957 2210 78-79 0.3750727425267186 2210 80-81 0.43778057560936645 2210 82-83 0.5156418527491269 2210 84-85 0.5300460420246509 2210 86-87 0.5295302972816884 2210 88-89 0.523521174421191 2210 90-91 0.5083527392984593 2210 92-93 0.6734650120278545 2210 94-95 0.5796668515487156 2210 96-97 0.5246158996754602 2210 98-99 0.5549281611226817 2210 100 0.5333571485442832 2211 1 0.37009220081124283 2211 2 4.013780720284558 2211 3 2.2214874584334794 2211 4 0.9173672973748381 2211 5 0.34930133194545476 2211 6 0.22422399486869438 2211 7 0.14047729209766402 2211 8 0.19782382734523196 2211 9 0.06991068695960934 2211 10-11 0.15818072076425693 2211 12-13 0.050417163917515495 2211 14-15 0.004775630549453069 2211 16-17 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-6.035885616455516E-4 2211 80-81 0.03227552117196808 2211 82-83 -0.4231118618406171 2211 84-85 -0.1981313920859904 2211 86-87 0.0181910660233342 2211 88-89 0.03399420877060777 2211 90-91 0.13920581348644134 2211 92-93 0.23641789999972218 2211 94-95 0.08216949555799857 2211 96-97 0.027614173770004413 2211 98-99 0.08552916227760932 2211 100 -0.11461783231504086 2212 1 0.5650830775164302 2212 2 4.159022125561588 2212 3 2.384868290825519 2212 4 1.2000710509589894 2212 5 0.5994157351661258 2212 6 0.6051197431236872 2212 7 0.35896644551382906 2212 8 0.3617904331899311 2212 9 0.20677422853576388 2212 10-11 0.2899388376003529 2212 12-13 0.4055779800903707 2212 14-15 0.40572362052831323 2212 16-17 0.42127462335396615 2212 18-19 0.5573580721344626 2212 20-21 0.6571641802600112 2212 22-23 0.4138523577088691 2212 24-25 0.5286631023560631 2212 26-27 0.4442165321414109 2212 28-29 0.3795046282416976 2212 30-31 0.40103200323516575 2212 32-33 0.41019775323506735 2212 34-35 0.47634334405587 2212 36-37 0.4600272862525472 2212 38-39 0.5349287448318591 2212 40-41 0.42769356543623616 2212 42-43 0.39524639482278445 2212 44-45 0.4136214473880244 2212 46-47 0.3910673644380438 2212 48-49 0.42880946564653755 2212 50-51 0.4238836603658953 2212 52-53 0.38125360386929685 2212 54-55 0.23660946284841344 2212 56-57 0.39657291860748245 2212 58-59 0.2570037564872294 2212 60-61 0.3550226519399047 2212 62-63 0.4812191102213106 2212 64-65 0.49478992809513755 2212 66-67 0.4547750835797828 2212 68-69 0.35776238682333883 2212 70-71 0.5281038352373102 2212 72-73 0.592798437914702 2212 74-75 0.5180601776486355 2212 76-77 0.5013105518215042 2212 78-79 0.4229079024453952 2212 80-81 0.29749911430198495 2212 82-83 0.6620707488698372 2212 84-85 0.6566762584483783 2212 86-87 0.6394711511740852 2212 88-89 0.6197622308982105 2212 90-91 0.4553118681682875 2212 92-93 0.4804519168560972 2212 94-95 0.5100123793880513 2212 96-97 0.43752960227522664 2212 98-99 0.5192873442801016 2212 100 0.5832186434865108 2213 1 -0.557566489783639 2213 2 -25.873474181676226 2213 3 -14.777781401652929 2213 4 -6.032695015685981 2213 5 -2.0234553978651135 2213 6 -1.2464099366700196 2213 7 -1.014723771378577 2213 8 -0.7978059499894314 2213 9 -0.8619562123424842 2213 10-11 -0.9141232008043758 2213 12-13 -1.0532842318950415 2213 14-15 -1.1904864997263829 2213 16-17 -1.2112513094335924 2213 18-19 -1.1390678765090243 2213 20-21 -1.2031869242305575 2213 22-23 -1.180193428369627 2213 24-25 -1.130507987279998 2213 26-27 -1.2027724062624756 2213 28-29 -1.170918787625915 2213 30-31 -1.1114209505573243 2213 32-33 -1.0130403610778345 2213 34-35 -1.0309439152886952 2213 36-37 -1.1376868522281143 2213 38-39 -1.1106467210403395 2213 40-41 -1.0804895538420638 2213 42-43 -1.1725293522835116 2213 44-45 -1.098689115998127 2213 46-47 -1.1282688354801778 2213 48-49 -1.0757392037072933 2213 50-51 -1.2212357389447845 2213 52-53 -1.3834998155867595 2213 54-55 -1.1859344330365573 2213 56-57 -1.2043526711913088 2213 58-59 -1.2361948849100983 2213 60-61 -1.2030581340134106 2213 62-63 -1.1214083839578919 2213 64-65 -1.1323820210991329 2213 66-67 -1.1815555417279526 2213 68-69 -1.1385138693416934 2213 70-71 -1.1636343917361316 2213 72-73 -1.2474976298151432 2213 74-75 -1.387365157620792 2213 76-77 -1.2907339449196797 2213 78-79 -1.2478181947430969 2213 80-81 -1.1493213548665793 2213 82-83 -1.2465997315647783 2213 84-85 -1.099583286403039 2213 86-87 -1.0674893926007911 2213 88-89 -1.094200374147313 2213 90-91 -0.9735953663075065 2213 92-93 -1.0250975536393625 2213 94-95 -1.2835977859308656 2213 96-97 -1.3622038720924081 2213 98-99 -1.192289465173367 2213 100 -1.129066154016602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 10.0 4 25.0 5 41.0 6 31.0 7 41.0 8 38.0 9 81.0 10 383.0 11 1689.0 12 4775.0 13 8699.0 14 11511.0 15 13180.0 16 14234.0 17 15022.0 18 15612.0 19 16713.0 20 18450.0 21 20826.0 22 23023.0 23 24754.0 24 25920.0 25 27579.0 26 30078.0 27 34431.0 28 40820.0 29 49173.0 30 59339.0 31 73500.0 32 93192.0 33 124755.0 34 173184.0 35 246425.0 36 377810.0 37 646016.0 38 655060.0 39 4086.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.325205568859246 20.939579708602587 10.537585062214257 30.197629660323912 2 12.791881225166152 22.885559396325927 36.6067239218621 27.71583545664582 3 14.95810930940876 27.541524090041303 29.20638172624306 28.293984874306876 4 10.333098859615731 19.129687354733008 38.25965537626015 32.27755840939111 5 11.427071613940084 38.973411017172474 35.509190580963335 14.090326787924104 6 29.052169814002905 40.46390639879004 17.968545708476206 12.51537807873085 7 25.527489387745934 33.56837744811787 23.63925214491061 17.264881019225587 8 24.13923804075645 37.133501674934585 21.090242913772016 17.637017370536945 9 24.56599047033799 16.903340645935188 22.14160149841073 36.389067385316096 10-11 22.720460227323574 27.995811010451593 29.51588146802044 19.767847294204394 12-13 23.589782194118236 25.45586502226069 29.72015688817857 21.23419589544251 14-15 21.145336106834304 27.051724482837646 26.792121142142395 25.01081826818566 16-17 20.53803103178909 30.517347383522004 27.588175719067348 21.35644586562156 18-19 20.466143290636868 29.52844398709719 29.60874878389202 20.396663938373923 20-21 21.777254639687666 28.76123001703045 28.77475682880302 20.686758514478864 22-23 21.084659598205473 28.72518390625321 29.046512154243175 21.143644341298145 24-25 21.16701383034845 28.590802001613685 28.93034580169372 21.311838366344148 26-27 21.034407445838994 28.588099183122484 28.952602157931995 21.424891213106527 28-29 20.78089564262856 28.592518726691964 29.246393740091044 21.380191890588435 30-31 21.372909683153633 28.905576997101974 28.672513489225988 21.048999830518405 32-33 20.6766694404724 28.77861451911891 29.31250780571385 21.232208234694838 34-35 20.836396480025652 28.440051873084606 29.303083038508206 21.42046860838154 36-37 21.271993565832254 28.824632969145643 28.725401543463526 21.177971921558584 38-39 21.053206759914488 28.820940782423566 28.958537364471137 21.167315093190812 40-41 21.22670585729918 28.57437057531011 28.885070242472743 21.313853324917968 42-43 20.932173583561887 28.653900297421963 28.976933352395474 21.436992766620676 44-45 21.232355137540857 28.387640803235172 28.895710396647928 21.484293662576043 46-47 21.284374924344203 28.872901417820344 28.607505265643514 21.235218392191936 48-49 21.178669059314352 28.678875649751046 28.70596487559045 21.43649041534415 50-51 21.39405798293955 28.559809215998527 28.45029754297 21.595835258091924 52-53 21.101251435643686 28.726471322041412 28.52882358452463 21.643453657790268 54-55 21.371812007061365 28.68352379211284 28.396370645288403 21.548293555537388 56-57 21.36446058118948 28.54694560093976 28.41761617017872 21.670977647692034 58-59 20.97978440960701 28.29261643428521 28.878625408622145 21.84897374748564 60-61 21.47503813332234 28.599281277876358 28.294793606407975 21.630886982393324 62-63 21.003543797477235 28.785933580112626 28.566726899528078 21.643795722882057 64-65 21.05637580925467 28.276536237381134 28.67671181639011 21.99037613697408 66-67 21.142442501028082 28.507167108075755 28.464640255104978 21.885750135791188 68-69 21.340070707336544 28.496354975392794 28.496004070496795 21.667570246773867 70-71 21.425590004310845 28.393264973874377 28.30255648796393 21.878588533850845 72-73 21.14906927104917 28.594234178976325 28.56253090060821 21.694165649366305 74-75 21.393273905769274 28.21119733769005 28.42329577354084 21.97223298299984 76-77 21.276576334599582 28.561275765814397 28.357136954931743 21.805010944654278 78-79 21.3138701753635 28.500208937324768 28.503068448990348 21.682852438321387 80-81 21.39441172213865 28.31882392670593 28.330419756768254 21.95634459438716 82-83 20.989822609184998 28.51128929580286 28.58714126657705 21.911746828435085 84-85 21.122586437580377 28.19049326517255 28.440210699414063 22.24670959783301 86-87 21.20614732156327 28.457008815268104 28.304497590063015 22.032346273105606 88-89 20.993409349212925 28.141698202538624 28.64741237448144 22.217480073767014 90-91 21.224659988678425 28.14210176122979 28.397074072131467 22.236164177960323 92-93 20.91605752925763 28.427080941499476 28.519278188176756 22.13758334106614 94-95 20.802210580101868 27.993015897183398 28.68388357232579 22.520889950388945 96-97 20.953306942530542 28.176789377818817 28.52292309246697 22.34698058718367 98-99 20.872295181374863 28.069720607832018 28.552988142571202 22.50499606822192 100 21.0247977770223 27.943541929483956 28.383916003360316 22.647744290133428 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21.0 1 20.0 2 19.0 3 20.0 4 20.5 5 25.5 6 52.5 7 112.5 8 176.0 9 187.5 10 182.5 11 199.0 12 214.0 13 255.5 14 320.5 15 399.0 16 485.0 17 587.0 18 765.0 19 994.0 20 1319.0 21 1879.0 22 2769.0 23 4145.0 24 6132.5 25 9218.5 26 14079.0 27 20640.5 28 29051.5 29 39069.5 30 49801.5 31 60467.0 32 71077.0 33 81018.5 34 89709.5 35 97926.0 36 105622.5 37 112188.5 38 117033.5 39 119972.5 40 121136.0 41 120400.0 42 118185.0 43 114555.5 44 109694.5 45 104715.0 46 99523.0 47 94636.5 48 90246.5 49 86781.5 50 86772.0 51 81649.0 52 71187.5 53 64374.5 54 59645.5 55 55342.0 56 51128.0 57 46682.5 58 41586.5 59 36749.0 60 31893.5 61 26757.5 62 21975.5 63 17670.5 64 14018.5 65 11178.5 66 8808.5 67 6713.5 68 5114.0 69 3814.0 70 2703.5 71 1998.0 72 1512.5 73 1093.0 74 791.5 75 536.5 76 330.5 77 200.5 78 115.5 79 56.5 80 31.5 81 15.0 82 8.5 83 9.0 84 6.0 85 2.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7768072705483994 2 5.494096854111223 3 0.0 4 0.005577941381799914 5 0.15604203312104414 6 0.05230635597650107 7 0.034309601706920226 8 0.04030851979678051 9 0.03841412461050884 10-11 0.039975246569566046 12-13 0.03844920600284721 14-15 0.04093998485887106 16-17 0.032274880951295104 18-19 0.026188259380588903 20-21 0.02169784116127828 22-23 0.021908329515308467 24-25 0.018014294965750036 26-27 0.011629481560167746 28-29 0.01769856243470476 30-31 0.02269766084292166 32-33 0.0013330929088578411 34-35 0.0033502729683137847 36-37 0.0037361682840357916 38-39 0.005349912331600547 40-41 0.006069080874537013 42-43 0.00654267967110493 44-45 0.007560040048917494 46-47 0.015260405667188445 48-49 0.023592236347549953 50-51 0.020680480783465717 52-53 0.026205800076758084 54-55 0.022487172488891476 56-57 0.015014835920819895 58-59 0.01357649883494696 60-61 0.011103260675092281 62-63 0.01627776604500101 64-65 0.018066917054257583 66-67 0.019294765786100333 68-69 0.02592514893805117 70-71 0.025451550141483252 72-73 0.02283798641227512 74-75 0.02308355615864367 76-77 0.01727758572664439 78-79 0.013225684911563318 80-81 0.012331109406935029 82-83 0.007910853972301136 84-85 0.01026130725897154 86-87 0.008138883022500504 88-89 0.006612842455781659 90-91 0.007174144733195487 92-93 0.003630924107020699 94-95 0.0014207963897037517 96-97 9.121162007974703E-4 98-99 7.893313276131954E-4 100 0.11092736257390773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2850514.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.24810966620718 #Duplication Level Percentage of deduplicated Percentage of total 1 89.06417707915898 75.92552734974642 2 8.344550070622443 14.227142390711581 3 1.6089666523040855 4.114840968746668 4 0.4876553050781112 1.662867717064262 5 0.19545012287078747 0.8330876754381279 6 0.09223247477639795 0.4717586474714437 7 0.053878274211483834 0.32151147200245894 8 0.03312199008775162 0.22588696346909418 9 0.02508505221578533 0.19246079540464212 >10 0.09128877649791986 1.231334436872621 >50 0.0015658645615229502 0.0862378799688697 >100 0.001533628819034649 0.25250800963507336 >500 3.298058638863917E-4 0.18707235336340095 >1k 1.6490293181827524E-4 0.26776334010530123 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.508139233836424E-5 0.0 3 0.0 0.0 0.0 3.508139233836424E-5 0.0 4 0.0 0.0 0.0 3.508139233836424E-5 0.0 5 0.0 0.0 0.0 3.508139233836424E-5 0.0 6 7.016278467672848E-5 0.0 0.0 3.508139233836424E-5 0.0 7 1.0524417701509271E-4 0.0 0.0 3.508139233836424E-5 0.0 8 1.0524417701509271E-4 0.0 0.0 3.508139233836424E-5 0.0 9 1.754069616918212E-4 0.0 0.0 3.508139233836424E-5 0.0 10-11 1.754069616918212E-4 0.0 0.0 3.508139233836424E-5 0.0 12-13 1.754069616918212E-4 0.0 0.0 3.508139233836424E-5 0.0 14-15 1.754069616918212E-4 0.0 0.0 3.508139233836424E-5 0.0 16-17 1.754069616918212E-4 0.0 0.0 7.016278467672848E-5 0.0 18-19 1.929476578610033E-4 0.0 0.0 7.016278467672848E-5 0.0 20-21 2.1048835403018542E-4 0.0 0.0 7.016278467672848E-5 0.0 22-23 2.1048835403018542E-4 0.0 0.0 1.4032556935345697E-4 0.0 24-25 2.1048835403018542E-4 0.0 0.0 2.2802905019936754E-4 0.0 26-27 2.1048835403018542E-4 0.0 0.0 4.560581003987351E-4 0.0 28-29 2.1048835403018542E-4 0.0 0.0 8.244127199515596E-4 0.0 30-31 2.1048835403018542E-4 0.0 0.0 0.002631104425377318 0.0 32-33 2.1048835403018542E-4 3.508139233836424E-5 0.0 0.007472336568071583 0.0 34-35 2.4556974636854965E-4 5.262208850754636E-5 0.0 0.01627776604500101 0.0 36-37 2.4556974636854965E-4 7.016278467672848E-5 0.0 0.029906886968455514 0.0 38-39 2.4556974636854965E-4 7.016278467672848E-5 0.0 0.05137669907953443 0.0 40-41 2.4556974636854965E-4 7.016278467672848E-5 0.0 0.07586351093171267 0.0 42-43 2.4556974636854965E-4 7.016278467672848E-5 0.0 0.10184128195827138 0.0 44-45 2.4556974636854965E-4 7.016278467672848E-5 0.0 0.13441435474444258 0.0 46-47 2.4556974636854965E-4 7.016278467672848E-5 0.0 0.1724425840392294 0.0 48-49 2.4556974636854965E-4 7.016278467672848E-5 0.0 0.2143297664912363 0.0 50-51 2.4556974636854965E-4 7.016278467672848E-5 0.0 0.25897083824180483 0.0 52-53 2.4556974636854965E-4 8.77034808459106E-5 0.0 0.30717267131471726 0.0 54-55 2.4556974636854965E-4 1.0524417701509271E-4 0.0 0.3633028990561 0.0 56-57 2.4556974636854965E-4 1.0524417701509271E-4 0.0 0.42995754449899215 0.0 58-59 2.4556974636854965E-4 1.0524417701509271E-4 0.0 0.5003483582259199 0.0 60-61 2.4556974636854965E-4 1.0524417701509271E-4 0.0 0.5775274213703213 0.0 62-63 2.4556974636854965E-4 1.0524417701509271E-4 0.0 0.66119654209732 0.0 64-65 2.4556974636854965E-4 1.0524417701509271E-4 0.0 0.7542148538824928 0.0 66-67 2.631104425377318E-4 1.2278487318427485E-4 0.0 0.8607395017179358 0.0 68-69 2.8065113870691394E-4 1.4032556935345697E-4 0.0 0.9838927295217634 0.0 70-71 2.8065113870691394E-4 1.4032556935345697E-4 0.0 1.1212363805264594 0.0 72-73 2.8065113870691394E-4 1.4032556935345697E-4 0.0 1.2651577925945987 0.0 74-75 2.8065113870691394E-4 1.754069616918212E-4 0.0 1.4161656459150875 0.0 76-77 2.8065113870691394E-4 2.1048835403018542E-4 0.0 1.5870120266029215 0.0 78-79 2.9819183487609605E-4 2.1048835403018542E-4 0.0 1.7772584172538708 0.0 80-81 3.1573253104527816E-4 2.1048835403018542E-4 0.0 1.9882554514729625 0.0 82-83 3.1573253104527816E-4 2.1048835403018542E-4 0.0 2.215460088952378 0.0 84-85 3.1573253104527816E-4 2.1048835403018542E-4 0.0 2.4503300106577273 0.0 86-87 3.1573253104527816E-4 2.1048835403018542E-4 0.0 2.71170392427471 0.0 88 3.1573253104527816E-4 2.1048835403018542E-4 0.0 2.923683237479276 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6740 0.0 24.079685 1 ATCAACG 7345 0.0 21.246456 3 TCAACGC 7385 0.0 20.940432 4 CAACGCA 7445 0.0 20.834806 5 AACGCAG 7660 0.0 20.350931 6 TATCAAC 7725 0.0 20.215666 2 ACCGTAC 155 0.0010411046 18.174568 8 GTACCGT 130 0.008087021 18.059332 6 ACGCAGA 8640 0.0 17.987001 7 CGCAGAG 8670 0.0 17.924763 8 GTACATG 9060 0.0 16.594793 1 GTACTAG 605 0.0 16.457645 1 TACATGG 9270 0.0 16.148928 2 GGTATCA 5070 0.0 16.005629 1 ACATGGG 9080 0.0 15.892521 3 GTATAAG 1175 0.0 15.676784 1 GAGTACT 3435 0.0 15.649773 12-13 GCAGAGT 10095 0.0 15.347474 9 TATACTG 1700 0.0 14.930869 5 CATGGGG 4065 0.0 14.916585 4 >>END_MODULE