FastQCFastQC Report
Wed 25 May 2016
SRR1295119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204879
Sequences flagged as poor quality0
Sequence length100
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46362.2627990179569406No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36021.7581108849613676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18990.9268885537317149No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17380.848305585247878No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17140.836591353921095No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC15590.7609369432689539No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13210.6447708159450213No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12550.6125566797963676No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11030.5383665480600746No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10310.5032238540797251No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9900.4832120422298039No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT8640.42171232776419254No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC6970.34020080144865994No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC6540.31921280365484017No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT6100.2977367128890711No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6070.2962724339732232No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6020.29383196911347675No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA5370.2621059259367724No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5290.25820118216117804No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5150.2513678805538879No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4910.23965364922710475No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4870.2377012773393076No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4740.23135606870396674No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA4470.2181775584613357No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4440.21671327954548783No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC3980.19426100283582015No Hit
CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3930.19182053797607368No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3800.18547532934073283No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3530.1722968190981018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3000.14642789158478905No Hit
GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2780.13568984620190455No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2710.13227319539825946No Hit
CCCNTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2630.12836845162266508No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG2590.1264160797348679No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2590.1264160797348679No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2460.12007087109952705No Hit
ANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.11372566246418618No Hit
GTANATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2290.11177329057638899No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT2210.10786854680079462No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.10738045382884531No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT2090.10201143113740305No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAG250.001922880372.5897141
ACATGAA1250.048.206073
GTACAAA1055.458787E-946.0887031
TACATGA1800.040.327622
GTACATA1602.2288441E-730.2457141
GTAAAAA1055.338252E-428.805441
AGTACTC1255.158803E-627.836495
CATGAAA1206.4580025E-527.3898124
TGTACTG750.00939147726.5109445
TGTTTCA1159.277163E-525.9719588
GTACAAG1250.001477349324.1965711
GAACAAG1250.001477349324.1965711
CAAGAAA1851.8847131E-623.6884884
ACAAGAA1902.3764896E-623.0651053
GAACAAA2203.5831454E-621.9968831
GTACATT1703.4974757E-421.3499161
TACATAG1754.2536083E-420.7399182
AAGTACT1500.00425873620.1638092
AAAAGTA1550.005148332619.5133652
GGAAGGA1300.00575099219.1459948
Produced by FastQC (version 0.11.5)