##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295119_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 204879 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.84914998608935 27.0 22.0 33.0 6.0 33.0 2 21.84263394491383 27.0 6.0 33.0 2.0 33.0 3 24.376993249674197 27.0 15.0 33.0 15.0 33.0 4 24.64114428516344 33.0 15.0 37.0 2.0 37.0 5 26.791145017302895 33.0 15.0 37.0 2.0 37.0 6 27.084156990223498 33.0 15.0 37.0 6.0 37.0 7 26.933980544614137 33.0 15.0 37.0 6.0 37.0 8 27.57535911440411 33.0 15.0 37.0 6.0 37.0 9 28.22132087720069 33.0 15.0 37.0 6.0 37.0 10-11 28.017097896807385 33.0 18.5 37.0 6.0 37.0 12-13 26.95098570375685 33.0 15.0 37.0 4.0 37.0 14-15 28.55701658051826 33.0 15.0 37.0 6.0 40.0 16-17 28.88478565397137 33.0 22.0 37.0 6.0 40.0 18-19 28.971544179735353 33.0 22.0 37.0 6.0 40.0 20-21 28.508978470219006 33.0 18.5 37.0 6.0 40.0 22-23 29.34482060142816 33.0 22.0 37.0 6.0 40.0 24-25 29.596059137344483 33.0 24.5 37.0 6.0 40.0 26-27 28.091478384802734 33.0 18.5 37.0 4.0 40.0 28-29 28.800274796343206 33.0 22.0 37.0 6.0 40.0 30-31 29.445436086665786 33.0 24.5 37.0 6.0 40.0 32-33 28.05219178149054 33.0 22.0 37.0 6.0 40.0 34-35 27.88813397175894 33.0 22.0 37.0 6.0 40.0 36-37 28.11928016048497 33.0 22.0 37.0 6.0 40.0 38-39 28.1276509549539 33.0 22.0 37.0 6.0 40.0 40-41 27.281634525744465 33.0 15.0 37.0 6.0 40.0 42-43 27.67309973203696 33.0 22.0 37.0 6.0 40.0 44-45 27.370245364337 33.0 22.0 37.0 6.0 40.0 46-47 26.817406859658625 33.0 15.0 37.0 6.0 40.0 48-49 26.583410207976414 33.0 15.0 37.0 6.0 40.0 50-51 26.646732949692257 33.0 15.0 37.0 6.0 40.0 52-53 26.999746191654587 33.0 18.5 37.0 6.0 40.0 54-55 26.822353681929336 33.0 22.0 37.0 6.0 40.0 56-57 26.62416109020446 33.0 18.5 37.0 6.0 40.0 58-59 26.38554707900761 27.0 15.0 37.0 6.0 37.0 60-61 26.256771069753366 27.0 18.5 37.0 6.0 37.0 62-63 25.88064662556924 27.0 15.0 37.0 6.0 37.0 64-65 25.613127748573547 27.0 15.0 37.0 6.0 37.0 66-67 25.779508880851626 27.0 15.0 33.0 6.0 37.0 68-69 25.25250513717853 27.0 15.0 33.0 6.0 37.0 70-71 25.111495077582376 27.0 15.0 33.0 6.0 37.0 72-73 24.85867267997208 27.0 15.0 33.0 6.0 37.0 74-75 24.869159357474413 27.0 15.0 33.0 6.0 37.0 76-77 24.447998574768523 27.0 15.0 33.0 6.0 37.0 78-79 24.028333797021656 27.0 15.0 33.0 6.0 37.0 80-81 24.119853669727007 27.0 15.0 33.0 6.0 37.0 82-83 24.30169026596186 27.0 15.0 33.0 6.0 37.0 84-85 24.062912743619407 27.0 15.0 33.0 6.0 33.0 86-87 23.583861693975468 27.0 15.0 33.0 6.0 33.0 88-89 23.460471790666688 27.0 15.0 33.0 6.0 33.0 90-91 23.623040916833837 27.0 15.0 33.0 6.0 33.0 92-93 23.337157541768555 27.0 15.0 33.0 6.0 33.0 94-95 23.368083112471265 27.0 15.0 33.0 6.0 33.0 96-97 23.542007721630817 27.0 15.0 33.0 6.0 33.0 98-99 23.500956662225022 27.0 15.0 33.0 6.0 33.0 100 22.885571483656207 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.277778104057511 1101 2 10.622395669568718 1101 3 8.286446700092416 1101 4 12.310390576185057 1101 5 10.569190806873348 1101 6 9.357749619589221 1101 7 8.813042211517168 1101 8 8.619740014023222 1101 9 7.143527593603256 1101 10-11 7.802954758936838 1101 12-13 8.798013349563686 1101 14-15 7.752838835852849 1101 16-17 8.051751757140938 1101 18-19 7.993102895603425 1101 20-21 6.762521808279086 1101 22-23 7.18633484246207 1101 24-25 7.075120267767847 1101 26-27 8.099535961908852 1101 28-29 7.998420163995402 1101 30-31 4.8611587774626805 1101 32-33 6.322217437841314 1101 34-35 8.273943842690393 1101 36-37 7.906211850314122 1101 38-39 7.985073968965338 1101 40-41 8.64281588214379 1101 42-43 8.364308905466125 1101 44-45 8.613192469481788 1101 46-47 7.22083007496169 1101 48-49 5.590317211058256 1101 50-51 6.701735663307513 1101 52-53 5.633545108475904 1101 54-55 4.028808864720364 1101 56-57 6.230096728704936 1101 58-59 6.613423244884192 1101 60-61 6.590828203041156 1101 62-63 6.065814907174531 1101 64-65 6.960534533305257 1101 66-67 6.056194990612347 1101 68-69 6.652813402829985 1101 70-71 5.887270868828146 1101 72-73 5.551086106647865 1101 74-75 4.482633384951551 1101 76-77 4.470925520660863 1101 78-79 4.705528280706272 1101 80-81 4.7056602396621905 1101 82-83 4.506615842699794 1101 84-85 3.9251393797754197 1101 86-87 4.082354025305072 1101 88-89 4.012818540072168 1101 90-91 3.724974406164172 1101 92-93 3.6542913731243054 1101 94-95 3.5149903091484838 1101 96-97 3.5524513568536022 1101 98-99 3.5386451196082227 1101 100 3.543425511729147 1102 1 6.914719976971423 1102 2 10.237637723314453 1102 3 8.17745279079622 1102 4 12.345373887476697 1102 5 10.215974969244133 1102 6 9.117207304042182 1102 7 6.334053790152019 1102 8 7.794575601006933 1102 9 6.228666996048535 1102 10-11 7.312120053620742 1102 12-13 8.93759347785532 1102 14-15 5.9480742321084605 1102 16-17 7.128562549472232 1102 18-19 7.264463092809077 1102 20-21 7.27107758275211 1102 22-23 6.8262388486517445 1102 24-25 7.030128229766174 1102 26-27 8.62095463706832 1102 28-29 8.21952080905221 1102 30-31 3.9598290787590926 1102 32-33 4.604634466621462 1102 34-35 5.8467623900980215 1102 36-37 5.488191194101518 1102 38-39 4.9865111771378565 1102 40-41 6.681856663769121 1102 42-43 7.132983615100599 1102 44-45 6.147876643548166 1102 46-47 5.320035674450686 1102 48-49 3.457387825436637 1102 50-51 5.083745145732639 1102 52-53 5.652899062035409 1102 54-55 4.890492759891888 1102 56-57 5.89909801521992 1102 58-59 5.591494012204912 1102 60-61 5.759073774460731 1102 62-63 4.375941489453009 1102 64-65 5.839066826630674 1102 66-67 3.3314822073316464 1102 68-69 5.455990105213182 1102 70-71 4.775004443270582 1102 72-73 4.539937059838511 1102 74-75 3.8837561616647314 1102 76-77 2.7341257941577553 1102 78-79 3.6709025845937084 1102 80-81 3.8022545735982973 1102 82-83 3.302093697548518 1102 84-85 2.444178452045172 1102 86-87 2.444928584696182 1102 88-89 3.405902847288349 1102 90-91 3.0353169070413415 1102 92-93 2.4796424657623817 1102 94-95 2.512104647043099 1102 96-97 2.8623530239251167 1102 98-99 2.7423980547516784 1102 100 2.409443522938922 1103 1 6.300441583530901 1103 2 10.566339096888033 1103 3 7.5637234607450665 1103 4 11.67187443196456 1103 5 9.81113680567815 1103 6 8.6025930668285 1103 7 8.092799912391833 1103 8 7.865226332633895 1103 9 6.58792374724046 1103 10-11 7.222220074668169 1103 12-13 8.614410191344891 1103 14-15 8.166368109136563 1103 16-17 7.940032483676131 1103 18-19 6.948314680974928 1103 20-21 7.724153252838217 1103 22-23 6.191450649854193 1103 24-25 4.228635234899915 1103 26-27 5.07050715040598 1103 28-29 6.538364207022507 1103 30-31 5.774339451481627 1103 32-33 7.581285545988575 1103 34-35 6.657941778258383 1103 36-37 3.840154553271333 1103 38-39 6.661637232940997 1103 40-41 6.226927904743892 1103 42-43 7.10446663531528 1103 44-45 5.518400633042752 1103 46-47 6.004829821434953 1103 48-49 4.896432638160267 1103 50-51 5.80036209165905 1103 52-53 6.145397483478167 1103 54-55 4.710370227245495 1103 56-57 5.4496644656166175 1103 58-59 5.200428188655582 1103 60-61 4.824629655387181 1103 62-63 2.4607054379508604 1103 64-65 4.628025161870479 1103 66-67 3.8520652211405917 1103 68-69 5.061190650616151 1103 70-71 3.1689345475011166 1103 72-73 4.049012620111945 1103 74-75 2.4927246545772377 1103 76-77 2.993684072661008 1103 78-79 1.8632898207001176 1103 80-81 2.960133175682909 1103 82-83 2.774054860464773 1103 84-85 2.4665276197565866 1103 86-87 2.132946191328866 1103 88-89 3.0792787635378787 1103 90-91 2.6648478238856903 1103 92-93 2.140650729166392 1103 94-95 2.612489448968482 1103 96-97 2.58109987824945 1103 98-99 2.137778601435791 1103 100 3.0186613821407633 1104 1 6.110652402754596 1104 2 9.651152566680043 1104 3 7.215569897570045 1104 4 11.784999933072896 1104 5 9.443693519350742 1104 6 8.15138804867069 1104 7 4.586112179523841 1104 8 6.67510700989655 1104 9 6.788220875005397 1104 10-11 6.800392974729487 1104 12-13 7.629573139984235 1104 14-15 7.613385040702845 1104 16-17 7.308437898004296 1104 18-19 6.7340218922186175 1104 20-21 7.025924228643529 1104 22-23 6.1633390618713655 1104 24-25 6.303065740017114 1104 26-27 8.627826869313683 1104 28-29 7.652586251072879 1104 30-31 6.259730669705458 1104 32-33 7.567763874526992 1104 34-35 7.8919157442065995 1104 36-37 7.06709885360619 1104 38-39 7.412901205020425 1104 40-41 7.32584551074304 1104 42-43 7.33514861428052 1104 44-45 6.876300458539145 1104 46-47 6.453806381355719 1104 48-49 5.670082593355286 1104 50-51 5.735180277764787 1104 52-53 6.350012396462176 1104 54-55 5.30505729982956 1104 56-57 6.536017811841209 1104 58-59 3.970385270200648 1104 60-61 4.385217779709212 1104 62-63 3.684732698244215 1104 64-65 3.100359265535051 1104 66-67 3.606781534869782 1104 68-69 4.431322235769194 1104 70-71 2.9123670806332207 1104 72-73 2.700163400661868 1104 74-75 3.295778467716886 1104 76-77 3.446476130388689 1104 78-79 3.7160559387022865 1104 80-81 2.8934633648070864 1104 82-83 3.0435403106369208 1104 84-85 3.079933741531807 1104 86-87 1.0662669478255893 1104 88-89 0.228323710007718 1104 90-91 2.188474197512061 1104 92-93 2.0924028397167866 1104 94-95 2.4746764036447075 1104 96-97 2.8342244360950346 1104 98-99 2.678268154800879 1104 100 1.0208261701424632 1105 1 6.4473490394512325 1105 2 -2.0002959847685062 1105 3 1.0014173834175288 1105 4 9.152765500838463 1105 5 9.345425121082346 1105 6 8.185986783269424 1105 7 5.267697261108925 1105 8 5.341806976596516 1105 9 5.025849912634435 1105 10-11 4.974635398971909 1105 12-13 5.23396874437984 1105 14-15 5.983481610799423 1105 16-17 6.199280388846788 1105 18-19 6.4442783024750305 1105 20-21 7.458258560977864 1105 22-23 2.1864992350315333 1105 24-25 4.3620400989914785 1105 26-27 7.206048647535464 1105 28-29 6.258530307016933 1105 30-31 5.57882823880303 1105 32-33 7.35704126380438 1105 34-35 6.561096563387419 1105 36-37 6.497285333792668 1105 38-39 4.397233539352754 1105 40-41 5.445942080839611 1105 42-43 6.110240189372096 1105 44-45 5.9870896693283555 1105 46-47 5.044998522547861 1105 48-49 5.360825184097873 1105 50-51 6.116648809233322 1105 52-53 5.966412662862446 1105 54-55 5.413898458670722 1105 56-57 5.471931897755294 1105 58-59 2.303502153317531 1105 60-61 4.2243286688201 1105 62-63 -0.26366890015738065 1105 64-65 -0.8315088663330776 1105 66-67 0.7825224606107071 1105 68-69 2.2966569011038622 1105 70-71 2.807471975738114 1105 72-73 2.5598537103521757 1105 74-75 2.198658920597339 1105 76-77 2.1768957108082674 1105 78-79 2.7748471474934924 1105 80-81 1.6453281666718915 1105 82-83 0.43559825269486296 1105 84-85 0.8427376043356674 1105 86-87 -0.7041959226372825 1105 88-89 1.1404282721122776 1105 90-91 1.2503456593835232 1105 92-93 1.5313805286944735 1105 94-95 0.6949727739410747 1105 96-97 1.5654931673637655 1105 98-99 1.3060570325897594 1105 100 1.3663473156636101 1106 1 6.016613108715301 1106 2 10.023513539041016 1106 3 6.217867599867745 1106 4 11.202549050622011 1106 5 9.754516030173253 1106 6 9.103735701018344 1106 7 6.583714577126241 1106 8 6.720594855384395 1106 9 6.64056852735305 1106 10-11 5.4032068275433325 1106 12-13 7.511025021436115 1106 14-15 7.708589835907645 1106 16-17 7.605124544690941 1106 18-19 7.030858389055123 1106 20-21 8.011288864008161 1106 22-23 5.871564170096466 1106 24-25 4.990827977779357 1106 26-27 7.656265097751909 1106 28-29 6.0312575797442065 1106 30-31 5.088568498543285 1106 32-33 6.978253385016501 1106 34-35 6.466940719231573 1106 36-37 5.420445506952845 1106 38-39 6.600696742815959 1106 40-41 6.274946409843938 1106 42-43 5.656776985908891 1106 44-45 3.5087346909733768 1106 46-47 5.411881639430977 1106 48-49 5.362989686262377 1106 50-51 6.062536255120765 1106 52-53 5.139572836022616 1106 54-55 4.8522101469824115 1106 56-57 4.388598564421962 1106 58-59 4.304584404399783 1106 60-61 4.795757240248673 1106 62-63 4.080486943998462 1106 64-65 4.642517107692893 1106 66-67 2.3355527636410116 1106 68-69 2.550985905432867 1106 70-71 2.5336624519285884 1106 72-73 2.5068234073218747 1106 74-75 1.9259316478700654 1106 76-77 2.5037355376480974 1106 78-79 3.0053666280129754 1106 80-81 2.1106961320398554 1106 82-83 1.5351653522619628 1106 84-85 2.525638037236103 1106 86-87 2.3758906574493004 1106 88-89 2.8731122547962613 1106 90-91 2.7568391658770324 1106 92-93 1.8874411347550826 1106 94-95 1.1581762371445379 1106 96-97 0.9713373232079228 1106 98-99 1.724888201420928 1106 100 2.989723939040232 1107 1 5.739297091399283 1107 2 9.932691028218507 1107 3 5.872297614297761 1107 4 10.744511532584493 1107 5 8.998614154271376 1107 6 7.282841040123682 1107 7 4.157711691123353 1107 8 6.593495288284828 1107 9 5.313007199791723 1107 10-11 4.198805673142179 1107 12-13 6.187619538030631 1107 14-15 5.823092000409808 1107 16-17 6.453566103132502 1107 18-19 5.796183064379793 1107 20-21 5.311101273820576 1107 22-23 5.3055612840935815 1107 24-25 4.5787067656581435 1107 26-27 6.792686454173268 1107 28-29 5.704735213221841 1107 30-31 4.41255118252597 1107 32-33 4.348166804929921 1107 34-35 6.328008540299397 1107 36-37 5.8709938475011825 1107 38-39 6.030263842383057 1107 40-41 6.796346121243651 1107 42-43 5.492621141753048 1107 44-45 3.69092805316674 1107 46-47 2.9353448428941817 1107 48-49 3.2279824712551637 1107 50-51 4.108135100719611 1107 52-53 2.75147759792738 1107 54-55 4.04285949763176 1107 56-57 4.079998275821673 1107 58-59 1.9148297146450943 1107 60-61 1.5816158261072601 1107 62-63 -0.5829473994358807 1107 64-65 1.2530943082700965 1107 66-67 3.2375873956756394 1107 68-69 3.7259498303967895 1107 70-71 2.5134171416832807 1107 72-73 1.1346444751429416 1107 74-75 2.1639835959220157 1107 76-77 1.9138509777635377 1107 78-79 2.5745296871760353 1107 80-81 1.5062228275665497 1107 82-83 1.273015280111892 1107 84-85 1.45371884531691 1107 86-87 1.6217492433078853 1107 88-89 1.7126216343056377 1107 90-91 0.6626256016634677 1107 92-93 1.6663588836728316 1107 94-95 1.7545543035226032 1107 96-97 1.7203416522122517 1107 98-99 1.3066198031525253 1107 100 0.04871383803013174 1108 1 4.180596163490648 1108 2 -2.647908543515971 1108 3 0.3965850280498415 1108 4 5.838806084095317 1108 5 6.541949866857625 1108 6 6.309483914261204 1108 7 4.097188255482145 1108 8 4.516093432467553 1108 9 3.8538113872768527 1108 10-11 2.853314357415318 1108 12-13 2.3803437826178317 1108 14-15 3.8074246199672714 1108 16-17 4.1205611067013805 1108 18-19 3.5298804234090575 1108 20-21 4.068555473201972 1108 22-23 4.0823968777171835 1108 24-25 2.6225682931385137 1108 26-27 4.124511341158755 1108 28-29 5.5347685227626435 1108 30-31 3.8639144944460284 1108 32-33 3.6265750847300637 1108 34-35 2.2300737318292576 1108 36-37 0.7201896600160538 1108 38-39 1.6515486389569354 1108 40-41 5.083360158835848 1108 42-43 2.2574819554575463 1108 44-45 2.8583180127194403 1108 46-47 4.25024848315114 1108 48-49 4.54174882861895 1108 50-51 1.2596102375266547 1108 52-53 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-5.155177431043349 2212 32-33 -16.525137029878337 2212 34-35 -13.054665992640624 2212 36-37 -17.201149711987508 2212 38-39 -23.04973615761694 2212 40-41 -9.328058991834027 2212 42-43 -13.371007176831021 2212 44-45 -16.337528190687735 2212 46-47 -16.061646307548298 2212 48-49 -16.378074559226647 2212 50-51 -7.730753788169277 2212 52-53 -1.3101743194767508 2212 54-55 -22.29714050236824 2212 56-57 -22.12222394640055 2212 58-59 -11.679172251923248 2212 60-61 -6.497686042132663 2212 62-63 -2.690430191962921 2212 64-65 -15.682962722211713 2212 66-67 -15.72415301730371 2212 68-69 -13.159497563900162 2212 70-71 -7.874173812102363 2212 72-73 -15.181009408829526 2212 74-75 -5.197550327381819 2212 76-77 -9.103100841312177 2212 78-79 -3.6660504504837483 2212 80-81 -1.3469236817746477 2212 82-83 -1.2260211014023668 2212 84-85 -3.9713450681540827 2212 86-87 -3.2811219464659622 2212 88-89 -2.721321335212547 2212 90-91 -3.386037131866523 2212 92-93 -4.500809257920089 2212 94-95 -2.551502726959203 2212 96-97 -3.6871214746314074 2212 98-99 -2.916743028705877 2212 100 -2.8979134972697693 2213 1 -20.667369575267383 2213 2 -16.487308971781495 2213 3 -19.48992460792446 2213 4 -18.371044022971063 2213 5 -20.8991636235064 2213 6 -21.27938118209854 2213 7 -10.4778438644322 2213 8 -10.119838045048505 2213 9 -6.009215022430499 2213 10-11 -17.186749882413373 2213 12-13 -15.70460268419159 2213 14-15 -22.960241332923523 2213 16-17 -17.323100563534165 2213 18-19 -17.930483602286873 2213 20-21 -16.770009837290537 2213 22-23 -19.579994271461974 2213 24-25 -7.2579599010085225 2213 26-27 -22.709535768048955 2213 28-29 -23.640820342333715 2213 30-31 -19.204115484140694 2213 32-33 -22.768499861736746 2213 34-35 -17.446435904145048 2213 36-37 -23.18345059694326 2213 38-39 -10.816402824283609 2213 40-41 -16.759209434311906 2213 42-43 -22.43737885824695 2213 44-45 -16.520183057944372 2213 46-47 -16.014655157105818 2213 48-49 -6.510906417633727 2213 50-51 -22.230753788169277 2213 52-53 -17.201855735405953 2213 54-55 -16.63714050236824 2213 56-57 -16.28222394640055 2213 58-59 -16.699614729799354 2213 60-61 -17.4139397294483 2213 62-63 -15.980725177213657 2213 64-65 -16.335794580618792 2213 66-67 -8.315745937657693 2213 68-69 -20.88516128071432 2213 70-71 -15.692138413872275 2213 72-73 -8.293133302634836 2213 74-75 -3.6126830707446516 2213 76-77 -2.937260133347575 2213 78-79 -4.973248090601743 2213 80-81 -4.241554950211228 2213 82-83 -4.185873608776998 2213 84-85 -3.0240589324608678 2213 86-87 -3.1304729789143373 2213 88-89 -2.911822810138805 2213 90-91 -2.611818842780977 2213 92-93 -3.743641116327172 2213 94-95 -4.021001252032953 2213 96-97 -3.4863250144544153 2213 98-99 -4.723380196847472 2213 100 -3.9090639397476465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66.0 3 95.0 4 561.0 5 1565.0 6 1808.0 7 1862.0 8 1740.0 9 1839.0 10 2280.0 11 2728.0 12 3527.0 13 4418.0 14 4597.0 15 5107.0 16 5370.0 17 4983.0 18 4933.0 19 4966.0 20 4951.0 21 5176.0 22 5475.0 23 5791.0 24 6228.0 25 6533.0 26 6683.0 27 6983.0 28 7486.0 29 7849.0 30 8865.0 31 9818.0 32 11134.0 33 12635.0 34 13924.0 35 14042.0 36 11756.0 37 6102.0 38 1002.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.64421204166314 21.10254890337878 11.176327377423998 22.076911677534085 2 12.44688763136621 27.564106709781733 32.240582053354885 27.74842360549717 3 22.745962355167197 34.72972340768387 24.710854208143385 17.813460029005547 4 13.848411063245303 25.973652993694202 25.71205594056315 34.46588000249734 5 10.632731787345366 45.11317784773644 30.845674633086507 13.408415731831685 6 30.388866103999828 32.02721954799577 18.32894424417726 19.254970103827144 7 21.562640799342716 38.96530704195489 20.152659616760754 19.319392541941642 8 27.387644961290114 37.0843907610267 19.648345058734083 15.879619218949104 9 26.510607018571918 23.88905166520168 17.440539526372334 32.159801789854065 10-11 21.25679612794128 35.63566210057615 20.331449482879123 22.776092288603447 12-13 26.237393537920777 32.95606326889279 20.90820602947141 19.89833716371502 14-15 27.52525784599825 31.33684853720462 19.771968268349468 21.365925348447657 16-17 20.665826150985573 34.812976432010935 26.626159461363645 17.895037955639843 18-19 23.949397640715937 33.19898231059379 26.08220924279311 16.769410805897163 20-21 21.844502061776492 32.66584919501996 25.099381069793186 20.390267673410367 22-23 19.871193225717796 33.7036614518245 20.755313797984932 25.66983152447277 24-25 21.685906413477777 35.25584915763892 18.328819458746963 24.729424970136343 26-27 24.221896592352635 29.8169821695075 20.43017451293205 25.530946725207816 28-29 23.684237990377692 28.691595612561184 24.97025642096035 22.65390997610077 30-31 26.035702256652073 32.0575237045429 22.650213825693786 19.25656021311124 32-33 19.07307135581408 31.80373130327561 25.741167640594004 23.382029700316302 34-35 16.08844054591117 33.49184960702994 26.382473378179284 24.037236468879602 36-37 24.020411691666098 31.682654777310333 22.50760985167993 21.789323679343642 38-39 26.2704457485329 28.425313189328673 21.917530278436757 23.38671078370167 40-41 21.34091137712504 32.60208916819763 22.119452136023593 23.937547318653742 42-43 25.982761861009017 30.29751712328767 22.386349398596725 21.333371617106582 44-45 27.696412781796038 28.358403605737255 21.6818948900464 22.26328872242031 46-47 21.964587504225058 30.810431346038847 28.067392943501208 19.157588206234887 48-49 24.391652294245436 30.097630712719464 26.782307422274453 18.728409570760647 50-51 20.684879142055212 30.76091282309078 26.359128230907796 22.19507980394621 52-53 19.03524402260764 31.726768200221585 21.70164860131827 27.536339175852508 54-55 20.981711211019952 33.47789623785941 19.29209486267379 26.248297688446854 56-57 23.639572532224022 28.423738191356378 20.821635689255928 27.115053587163672 58-59 23.310483154340265 26.739787239444485 25.248432223830196 24.70129738238505 60-61 25.566478137583807 29.830527457199146 22.999954182851152 21.603040222365895 62-63 18.955188958132585 30.30827561394725 25.371286817336696 25.365248610583468 64-65 16.290109239105455 31.860895055896304 26.223769081094332 25.625226623903906 66-67 23.010806546538273 29.817052555128416 22.570530560112196 24.601610338221114 68-69 25.916999961419258 27.03069741123214 21.725588034825552 25.326714592523054 70-71 21.250641338489068 30.18933563589493 22.071304326062744 26.488718699553253 72-73 25.17816670551536 28.467989505894252 22.407345874021136 23.94649791456925 74-75 26.71073420188706 26.710488106195214 21.57250225177558 25.006275440142144 76-77 21.441895514459723 29.223585220323905 26.864816118790845 22.469703146425527 78-79 23.93375934116969 28.533253198163184 26.147327848047823 21.385659612619303 80-81 20.664555148258994 29.175585796840696 25.64896795592226 24.51089109897805 82-83 19.186374532813318 30.255957939032292 22.020660922867695 28.53700660528669 84-85 20.909246121983387 31.306710348223497 20.004107517995134 27.77993601179798 86-87 23.000796324655436 27.201960183767227 21.54781010719755 28.249433384379785 88-89 22.971806780436115 25.62664575183968 25.328166912120846 26.073380555603364 90-91 24.711948564517154 28.30934740986304 23.29262500183628 23.686079023783524 92-93 19.59662720965782 29.047691373025515 24.796427311566653 26.559254105750007 94-95 17.200558984397066 30.351673883197805 25.870465926757326 26.577301205647807 96-97 22.991023736139592 28.610091236170295 22.882473568525363 25.51641145916475 98-99 24.748242726875855 26.650953808119997 22.156602098426916 26.44420136657723 100 20.95900094250707 29.0827246308514 24.60041627395539 25.35785815268615 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1886.0 1 1864.5 2 1626.5 3 1133.0 4 705.5 5 475.5 6 330.5 7 253.5 8 250.5 9 252.0 10 263.0 11 279.0 12 315.0 13 358.5 14 393.5 15 462.0 16 528.5 17 582.5 18 632.0 19 681.5 20 699.0 21 721.0 22 783.0 23 835.5 24 934.5 25 1072.5 26 1212.0 27 1376.5 28 1579.5 29 1789.0 30 1969.0 31 2150.0 32 2260.5 33 2362.0 34 2464.5 35 2603.0 36 2758.0 37 2870.0 38 3000.5 39 3079.5 40 3116.5 41 3186.0 42 3252.5 43 3330.0 44 3592.0 45 4100.0 46 5240.0 47 7362.5 48 10490.5 49 15926.5 50 23140.5 51 23112.5 52 15175.5 53 8833.0 54 5712.0 55 4091.5 56 3156.0 57 2480.0 58 1967.5 59 1565.5 60 1297.0 61 1106.5 62 907.5 63 739.5 64 615.0 65 517.0 66 429.0 67 336.0 68 286.0 69 250.0 70 194.0 71 140.0 72 101.5 73 79.0 74 63.0 75 45.5 76 27.0 77 17.0 78 12.5 79 7.0 80 9.0 81 9.0 82 3.0 83 1.5 84 2.0 85 2.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.777761508012046 2 24.528624212339967 3 6.1021383353101095 4 21.822148682881114 5 11.936313629020056 6 9.552467554019689 7 7.918820376905393 8 8.83692325714202 9 5.045905144011831 10-11 6.769605474450774 12-13 9.73940716227627 14-15 7.339454019201577 16-17 7.861225406215376 18-19 6.572171867297283 20-21 7.851219500290416 22-23 5.872734638493941 24-25 5.2045353598953525 26-27 9.898281424645766 28-29 6.46283904158064 30-31 4.356229774647475 32-33 8.956017942297649 34-35 6.90749173902645 36-37 6.837206351065752 38-39 6.180721303793947 40-41 7.80948755118875 42-43 6.50481503716828 44-45 6.435261788665506 46-47 6.137281029290459 48-49 4.0619097125620485 50-51 6.789861332786669 52-53 4.400646235094861 54-55 4.843590607138848 56-57 4.726204247385042 58-59 3.8774105691652148 60-61 4.122433241083762 62-63 2.999087266142455 64-65 4.427003255580123 66-67 2.553458382752747 68-69 5.115946485486555 70-71 2.490006296399338 72-73 1.5823974150596207 74-75 0.8326866101455005 76-77 1.2234050341909128 78-79 1.7339502828498772 80-81 2.0663415967473484 82-83 0.9447039472078641 84-85 1.371785297663499 86-87 0.3985279115966009 88-89 0.7392168060172102 90-91 0.3231175474304346 92-93 0.3782720532607051 94-95 0.6323244451603142 96-97 0.6279316084127705 98-99 0.4939500876126885 100 0.5696044982648295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 204879.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.30292008521515 #Duplication Level Percentage of deduplicated Percentage of total 1 91.43085148011623 53.30685627168392 2 4.440571619207363 5.177965844946426 3 1.3286726442851404 2.3239648499750416 4 0.6833703642931604 1.5936995095195399 5 0.41517994720642437 1.2103101641480003 6 0.28227215291433366 0.9874374464187615 7 0.19247585923667707 0.785533324758637 8 0.1515819649742782 0.7070136952204179 9 0.13462760339836413 0.7064264161978977 >10 0.7997856792026744 8.882344651780663 >50 0.07579015654300461 3.1169894035218526 >100 0.04487575058467378 5.81535683902734 >500 0.009972389018816395 3.840275137150128 >1k 0.009972389018816395 11.545826445651361 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4636 2.2627990179569406 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3602 1.7581108849613676 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1899 0.9268885537317149 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1738 0.848305585247878 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1714 0.836591353921095 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1559 0.7609369432689539 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1321 0.6447708159450213 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1255 0.6125566797963676 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1103 0.5383665480600746 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1031 0.5032238540797251 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 990 0.4832120422298039 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 864 0.42171232776419254 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 697 0.34020080144865994 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 654 0.31921280365484017 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 610 0.2977367128890711 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 607 0.2962724339732232 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 602 0.29383196911347675 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 537 0.2621059259367724 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 529 0.25820118216117804 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 515 0.2513678805538879 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 491 0.23965364922710475 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 487 0.2377012773393076 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 474 0.23135606870396674 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 447 0.2181775584613357 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 444 0.21671327954548783 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 398 0.19426100283582015 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 393 0.19182053797607368 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 380 0.18547532934073283 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 353 0.1722968190981018 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 300 0.14642789158478905 No Hit GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 278 0.13568984620190455 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 271 0.13227319539825946 No Hit CCCNTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 263 0.12836845162266508 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 259 0.1264160797348679 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 259 0.1264160797348679 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 246 0.12007087109952705 No Hit ANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 233 0.11372566246418618 No Hit GTANATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 229 0.11177329057638899 No Hit ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT 221 0.10786854680079462 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 220 0.10738045382884531 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 209 0.10201143113740305 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4404648597464845E-4 0.0 18-19 0.0 0.0 0.0 4.880929719492969E-4 0.0 20-21 0.0 0.0 0.0 0.0012202324298732422 0.0 22-23 0.0 0.0 0.0 0.0024404648597464843 0.0 24-25 0.0 0.0 0.0 0.0031726043176704298 0.0 26-27 0.0 0.0 0.0 0.004880929719492969 0.0 28-29 0.0 0.0 0.0 0.012202324298732421 0.0 30-31 4.880929719492969E-4 0.0 0.0 0.025868927513312736 0.0 32-33 4.880929719492969E-4 0.0 0.0 0.06271994689548466 0.0 34-35 4.880929719492969E-4 0.0 0.0 0.1188506386696538 0.0 36-37 4.880929719492969E-4 0.0 0.0 0.18864793365840327 0.0 38-39 4.880929719492969E-4 0.0 0.0 0.3013974101786908 0.0 40-41 4.880929719492969E-4 0.0 0.0 0.5112773881168885 0.0 42-43 4.880929719492969E-4 0.0 0.0 0.6245149576091253 0.0 44-45 4.880929719492969E-4 0.0 0.0 0.7370203876434382 0.0 46-47 4.880929719492969E-4 0.0 0.0 0.8954065570409852 0.0 48-49 4.880929719492969E-4 0.0 0.0 1.0223107297478025 0.0 50-51 4.880929719492969E-4 0.0 0.0 1.1597089013515294 0.0 52-53 4.880929719492969E-4 0.0 0.0 1.36397581011231 0.0 54-55 4.880929719492969E-4 0.0 0.0 1.5514035113408402 0.0 56-57 4.880929719492969E-4 0.0 0.0 1.7427359563449647 0.0 58-59 4.880929719492969E-4 0.0 0.0 1.961889700750199 0.0 60-61 4.880929719492969E-4 0.0 0.0 2.1759184689499653 0.0 62-63 4.880929719492969E-4 0.0 0.0 2.3938519809253265 0.0 64-65 4.880929719492969E-4 0.0 0.0 2.6793863695156652 0.0 66-67 4.880929719492969E-4 0.0 0.0 2.9554029451529926 0.0 68-69 4.880929719492969E-4 0.0 0.0 3.314639372507675 0.0 70-71 4.880929719492969E-4 0.0 0.0 3.9540411657612538 0.0 72-73 4.880929719492969E-4 0.0 0.0 4.417485442627111 0.0 74-75 4.880929719492969E-4 0.0 0.0 4.831144236354141 0.0 76-77 4.880929719492969E-4 0.0 0.0 5.339249020153359 0.0 78-79 4.880929719492969E-4 0.0 0.0 5.8034254364771405 0.0 80-81 4.880929719492969E-4 0.0 0.0 6.291762454912412 0.0 82-83 4.880929719492969E-4 0.0 0.0 7.036348283621065 0.0 84-85 4.880929719492969E-4 0.0 0.0 7.721630816237877 0.0 86-87 4.880929719492969E-4 0.0 0.0 8.329306566314752 0.0 88 4.880929719492969E-4 0.0 0.0 8.875970694897964 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTATAG 25 0.0019228803 72.589714 1 ACATGAA 125 0.0 48.20607 3 GTACAAA 105 5.458787E-9 46.088703 1 TACATGA 180 0.0 40.32762 2 GTACATA 160 2.2288441E-7 30.245714 1 GTAAAAA 105 5.338252E-4 28.80544 1 AGTACTC 125 5.158803E-6 27.83649 5 CATGAAA 120 6.4580025E-5 27.389812 4 TGTACTG 75 0.009391477 26.510944 5 TGTTTCA 115 9.277163E-5 25.971958 8 GTACAAG 125 0.0014773493 24.196571 1 GAACAAG 125 0.0014773493 24.196571 1 CAAGAAA 185 1.8847131E-6 23.688488 4 ACAAGAA 190 2.3764896E-6 23.065105 3 GAACAAA 220 3.5831454E-6 21.996883 1 GTACATT 170 3.4974757E-4 21.349916 1 TACATAG 175 4.2536083E-4 20.739918 2 AAGTACT 150 0.004258736 20.163809 2 AAAAGTA 155 0.0051483326 19.513365 2 GGAAGGA 130 0.005750992 19.145994 8 >>END_MODULE