FastQCFastQC Report
Wed 25 May 2016
SRR1295118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012110
Sequences flagged as poor quality0
Sequence length100
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63060.6230548062957584No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51920.5129877187262254No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22430.22161622748515475No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21730.21469998320340677No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21100.20847536334983352No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15620.15433105097272035No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15430.15245378466767445No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14850.1467231822627975No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13460.1329894971890407No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT13060.12903735759947044No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11550.11411803064884252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG2450.032.5777669
GATACGA1403.5109953E-421.3555536
TGATACG1454.605315E-420.5142545
TACTGTG4500.019.8890887
GTACATA5550.019.5951481
ATACGAC1557.075328E-419.2475037
CCTATAC1500.00766207318.2272243
TACATCG2351.5097317E-417.9893592
GTCTAAC1700.00886904217.7701111
GTATAGA2100.001468941817.2623921
ACGACTG1750.001563244317.1033259
GTGTATA2150.00171953616.860941
TGTGCGT2256.366463E-1116.63500810-11
GTGCGTT2104.274625E-1016.63500810-11
GTACTGT4901.2732926E-1116.2708996
GTACATT6900.015.7613151
AGTACTC4106.0608727E-915.7192555
TACGACT1950.003229979415.3419998
GTCCGTG2000.00381097214.965419
TGTACTG5003.45608E-1014.8728335
Produced by FastQC (version 0.11.5)