##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295118_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012110 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.641447075910722 33.0 27.0 33.0 15.0 33.0 2 22.8337996858049 33.0 6.0 33.0 2.0 33.0 3 25.126594935333117 33.0 15.0 33.0 15.0 33.0 4 25.70357767436346 33.0 15.0 37.0 2.0 37.0 5 27.481059371016983 33.0 15.0 37.0 2.0 37.0 6 28.117411150961853 33.0 15.0 37.0 6.0 37.0 7 27.757464109632352 33.0 15.0 37.0 6.0 37.0 8 28.482885259507366 33.0 22.0 37.0 6.0 37.0 9 29.45369080435921 33.0 27.0 37.0 15.0 37.0 10-11 29.017321733803637 35.0 24.5 37.0 10.5 37.0 12-13 27.865629230024403 33.0 15.0 37.0 4.0 37.0 14-15 29.601147108515875 33.0 27.0 37.0 10.5 40.0 16-17 30.01563515823379 37.0 27.0 38.5 6.0 40.0 18-19 30.119294839493733 37.0 27.0 40.0 10.5 40.0 20-21 29.665162877552834 33.0 27.0 38.5 6.0 40.0 22-23 30.45345318196639 37.0 27.0 40.0 15.0 40.0 24-25 30.573950953947694 37.0 27.0 40.0 15.0 40.0 26-27 29.172306369860983 33.0 24.5 38.5 4.0 40.0 28-29 29.845291025679025 33.0 27.0 37.0 10.5 40.0 30-31 30.51555117526751 33.0 27.0 40.0 15.0 40.0 32-33 29.219670292754742 33.0 24.5 37.0 6.0 40.0 34-35 28.94564622422464 33.0 22.0 37.0 6.0 40.0 36-37 29.23796869905445 33.0 22.0 37.0 6.0 40.0 38-39 29.313178409461422 33.0 24.5 37.0 6.0 40.0 40-41 28.449470413294996 33.0 22.0 37.0 6.0 40.0 42-43 29.065195976721895 33.0 22.0 37.0 6.0 40.0 44-45 28.871427018802304 33.0 22.0 37.0 6.0 40.0 46-47 28.42547104563733 33.0 22.0 37.0 6.0 40.0 48-49 28.29637638201381 33.0 22.0 37.0 6.0 40.0 50-51 28.250250960863937 33.0 22.0 37.0 6.0 40.0 52-53 28.47353153313375 33.0 22.0 37.0 6.0 40.0 54-55 28.40266324806592 33.0 22.0 37.0 6.0 40.0 56-57 28.362077738585725 33.0 22.0 37.0 6.0 40.0 58-59 28.171861754157156 33.0 22.0 37.0 6.0 40.0 60-61 28.094318305322545 33.0 22.0 37.0 10.5 40.0 62-63 27.917168588394542 33.0 22.0 37.0 6.0 40.0 64-65 27.524257244765884 33.0 22.0 37.0 6.0 38.5 66-67 27.72777168489591 33.0 22.0 37.0 6.0 37.0 68-69 27.259829959194157 33.0 22.0 37.0 6.0 37.0 70-71 27.321913625989268 33.0 22.0 37.0 10.5 37.0 72-73 27.288042307654308 30.0 22.0 37.0 10.5 37.0 74-75 27.34859402634101 27.0 22.0 35.0 10.5 37.0 76-77 27.079519518629397 27.0 22.0 33.0 10.5 37.0 78-79 26.61564701465256 27.0 22.0 33.0 6.0 37.0 80-81 26.50112537174813 27.0 22.0 33.0 6.0 37.0 82-83 26.638853978322516 27.0 22.0 33.0 10.5 37.0 84-85 26.513557320844573 27.0 22.0 33.0 10.5 37.0 86-87 26.224029502722036 27.0 22.0 33.0 10.5 37.0 88-89 26.120066494748595 27.0 22.0 33.0 6.0 37.0 90-91 26.295799369633734 27.0 22.0 33.0 10.5 37.0 92-93 26.19862761952752 27.0 22.0 33.0 6.0 37.0 94-95 26.09421307960597 27.0 22.0 33.0 10.5 37.0 96-97 26.188038355514717 27.0 22.0 33.0 6.0 37.0 98-99 26.223380857811897 27.0 22.0 33.0 10.5 37.0 100 25.81960952860855 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.255319626135581 1101 2 11.250756805395373 1101 3 8.482127008018299 1101 4 12.751308646186668 1101 5 10.720858420580115 1101 6 10.009414029194986 1101 7 9.391909057191949 1101 8 9.106474824740395 1101 9 7.1443396747520715 1101 10-11 7.695903963277967 1101 12-13 8.91436027656301 1101 14-15 7.912858837815833 1101 16-17 8.208239624097342 1101 18-19 8.038787133851276 1101 20-21 6.980574397269464 1101 22-23 7.248794834327384 1101 24-25 7.248288237313769 1101 26-27 8.473444180300863 1101 28-29 8.082773957839422 1101 30-31 5.016335097742957 1101 32-33 6.426432581417757 1101 34-35 8.578460979194563 1101 36-37 7.847732844940921 1101 38-39 8.054373773457836 1101 40-41 8.815729921678233 1101 42-43 8.564387525490023 1101 44-45 8.466705184321967 1101 46-47 7.3233971447261474 1101 48-49 5.35360397543457 1101 50-51 6.667191593301528 1101 52-53 5.921215855071882 1101 54-55 4.042475821367937 1101 56-57 6.205591021575639 1101 58-59 6.000589345706146 1101 60-61 6.441125561914735 1101 62-63 6.004073505301637 1101 64-65 6.443932548388268 1101 66-67 5.418325275061399 1101 68-69 6.735228295135901 1101 70-71 6.192060023774566 1101 72-73 5.189862036025335 1101 74-75 4.553764030692964 1101 76-77 4.713672602752531 1101 78-79 4.748406781967507 1101 80-81 4.585029602855208 1101 82-83 4.231567811091935 1101 84-85 4.057686517349737 1101 86-87 3.946018992841193 1101 88-89 3.872155394470294 1101 90-91 3.5313650443776616 1101 92-93 3.5585888615493992 1101 94-95 3.480660175488211 1101 96-97 3.579611077637672 1101 98-99 3.36659416330226 1101 100 3.4967819996184275 1102 1 6.698933676419777 1102 2 10.556744778018377 1102 3 8.172632410321697 1102 4 12.53149324153301 1102 5 10.66518889528092 1102 6 9.552211830196875 1102 7 6.408002768769361 1102 8 7.761720966561853 1102 9 6.320341116791848 1102 10-11 7.3329853370496885 1102 12-13 8.924989599001258 1102 14-15 6.024931475505028 1102 16-17 7.190706873869775 1102 18-19 7.4236147108736965 1102 20-21 6.9728948977367935 1102 22-23 6.476589757064396 1102 24-25 6.680552226628269 1102 26-27 8.377318199144543 1102 28-29 7.540173293128113 1102 30-31 3.759195847307275 1102 32-33 4.159632245967195 1102 34-35 4.748124892768214 1102 36-37 4.980319192418108 1102 38-39 4.490483045859861 1102 40-41 5.994940033513224 1102 42-43 6.801147835333499 1102 44-45 6.339675041601669 1102 46-47 5.406787014846909 1102 48-49 3.438052398034756 1102 50-51 4.906892347540097 1102 52-53 5.653398701513591 1102 54-55 5.264684434329379 1102 56-57 6.278349412200729 1102 58-59 5.98133357553067 1102 60-61 5.898267738445693 1102 62-63 4.39506323264396 1102 64-65 6.105010716960241 1102 66-67 3.724639432279371 1102 68-69 6.010365563704742 1102 70-71 5.1311176185204985 1102 72-73 4.92271160077804 1102 74-75 4.265574876640233 1102 76-77 3.144507869778419 1102 78-79 4.243530237866093 1102 80-81 4.463730386542302 1102 82-83 3.8863750386222904 1102 84-85 2.9296055125286173 1102 86-87 3.293153679693667 1102 88-89 3.161541809172647 1102 90-91 3.257096319665642 1102 92-93 3.1618763671038224 1102 94-95 3.1470303561764723 1102 96-97 3.008068704683197 1102 98-99 2.9649683609941313 1102 100 3.118433003859952 1103 1 6.7916366735611575 1103 2 10.984014506275553 1103 3 8.056917324828728 1103 4 12.032405726192476 1103 5 10.383121964764342 1103 6 9.00510384596086 1103 7 8.308519002658588 1103 8 7.974741352651272 1103 9 7.168887128119334 1103 10-11 7.676369295023047 1103 12-13 8.805140207035656 1103 14-15 8.780929251353413 1103 16-17 8.070226347474541 1103 18-19 7.376042346471468 1103 20-21 8.602281262986885 1103 22-23 7.170514114970061 1103 24-25 5.538059280847573 1103 26-27 6.317941212869979 1103 28-29 7.276221948062712 1103 30-31 6.413178466411413 1103 32-33 7.966157610604135 1103 34-35 7.3083001484533305 1103 36-37 4.01836629059887 1103 38-39 6.444773532167456 1103 40-41 6.8920480843747995 1103 42-43 7.568550670041763 1103 44-45 5.910448806130567 1103 46-47 6.3260494927200845 1103 48-49 5.627420709661163 1103 50-51 6.5391699766371225 1103 52-53 6.165768688868059 1103 54-55 4.904377298103515 1103 56-57 5.865540095891308 1103 58-59 5.652428779841763 1103 60-61 5.134535591478841 1103 62-63 2.6823185812012937 1103 64-65 4.941598391726227 1103 66-67 4.1031110761547005 1103 68-69 5.13656684638006 1103 70-71 3.7412787971761823 1103 72-73 4.255561010447007 1103 74-75 3.2045438632026197 1103 76-77 3.5780395442367876 1103 78-79 3.0215475715529827 1103 80-81 4.225985254443799 1103 82-83 3.464754206217748 1103 84-85 3.296994374904763 1103 86-87 2.8492860962846507 1103 88-89 2.929062190407919 1103 90-91 2.772331396539169 1103 92-93 2.320823732719589 1103 94-95 2.7555869057642575 1103 96-97 3.1403471903945785 1103 98-99 2.8812936985620574 1103 100 2.955893283127658 1104 1 6.246003054668005 1104 2 9.684892399049527 1104 3 7.352064001717668 1104 4 11.99206992610613 1104 5 9.25355387021986 1104 6 8.034763720562225 1104 7 3.764710555351243 1104 8 6.294723744419091 1104 9 6.372629802343031 1104 10-11 7.080012042332324 1104 12-13 7.0485419411749675 1104 14-15 6.914558770842312 1104 16-17 6.821200920226961 1104 18-19 6.5431084190603634 1104 20-21 7.055050446770146 1104 22-23 6.10520701582989 1104 24-25 5.8046025180309435 1104 26-27 8.079742250866222 1104 28-29 7.166106935686962 1104 30-31 6.118303351440357 1104 32-33 7.365607186082016 1104 34-35 7.62915822901434 1104 36-37 6.138668810575396 1104 38-39 7.170261428574062 1104 40-41 6.904996668258111 1104 42-43 7.035003425942623 1104 44-45 6.67729745207955 1104 46-47 6.3907652559448564 1104 48-49 5.1955411312354585 1104 50-51 5.445772650459379 1104 52-53 5.731898345121834 1104 54-55 4.5149462911637315 1104 56-57 5.640765344695154 1104 58-59 3.470852248773703 1104 60-61 3.7440527740672316 1104 62-63 3.173257366815754 1104 64-65 2.406347391768339 1104 66-67 2.7972023807814494 1104 68-69 3.704522854923635 1104 70-71 1.8838357179316603 1104 72-73 1.9508166859926988 1104 74-75 2.9478050935670197 1104 76-77 3.4032396637240296 1104 78-79 3.9111460416754653 1104 80-81 2.6262311163714926 1104 82-83 2.726450948694797 1104 84-85 2.4518320930447466 1104 86-87 0.42830726906218075 1104 88-89 -0.12315965836200604 1104 90-91 1.897541709437533 1104 92-93 1.9747002594190448 1104 94-95 2.049040354738363 1104 96-97 2.5351419387806544 1104 98-99 2.2618947123145077 1104 100 0.45508351697395355 1105 1 5.90150714038608 1105 2 -2.3948028416166522 1105 3 0.6716332297825858 1105 4 8.741596142895201 1105 5 8.858289975914126 1105 6 8.298388470133958 1105 7 5.522886896089066 1105 8 5.971334430771076 1105 9 5.0669354578431935 1105 10-11 4.942137792832391 1105 12-13 5.110918688875891 1105 14-15 5.883645544384528 1105 16-17 6.359206573451392 1105 18-19 6.376518198573624 1105 20-21 7.4020675155214875 1105 22-23 1.895792678580996 1105 24-25 4.055349781074593 1105 26-27 7.211717261793641 1105 28-29 6.120797958407373 1105 30-31 5.5348704222712435 1105 32-33 7.20405554704714 1105 34-35 6.276148538332972 1105 36-37 6.481476173264589 1105 38-39 4.320404014759443 1105 40-41 5.591411626231675 1105 42-43 5.935221542784827 1105 44-45 6.499094344061682 1105 46-47 5.571676285147166 1105 48-49 5.220801133260075 1105 50-51 6.1393926445424825 1105 52-53 5.840947545595501 1105 54-55 5.518757352304718 1105 56-57 5.32419576271516 1105 58-59 2.0154610470554495 1105 60-61 4.6010808918746555 1105 62-63 -0.4732079553921409 1105 64-65 -0.5654414174526003 1105 66-67 0.5669974945561229 1105 68-69 2.7235899316400243 1105 70-71 3.0726527469754537 1105 72-73 2.8647856984126854 1105 74-75 2.804841058715411 1105 76-77 1.6414547232421874 1105 78-79 3.141916328817519 1105 80-81 1.4965041724478034 1105 82-83 1.0246601070125436 1105 84-85 1.334233018403193 1105 86-87 -0.7080614680730832 1105 88-89 1.4958468368821158 1105 90-91 1.4337758254384987 1105 92-93 1.8115728743178465 1105 94-95 1.1057621123544052 1105 96-97 2.1673129370487487 1105 98-99 2.164528436890105 1105 100 2.178363909529338 1106 1 6.201244068965515 1106 2 10.433186248042269 1106 3 6.7963674516705055 1106 4 11.878036758083443 1106 5 9.960606144074696 1106 6 9.361864469852847 1106 7 6.460593673074779 1106 8 7.583610084033445 1106 9 6.90732366388605 1106 10-11 5.7126209895015485 1106 12-13 7.857094561142844 1106 14-15 7.758959093227496 1106 16-17 7.68599759597296 1106 18-19 7.249383948574096 1106 20-21 8.250533818636747 1106 22-23 6.182516119289254 1106 24-25 4.940475419046532 1106 26-27 7.672194437261329 1106 28-29 6.235148741828361 1106 30-31 5.473327285206285 1106 32-33 7.511965449359323 1106 34-35 6.728527577713908 1106 36-37 5.9627169016590145 1106 38-39 7.014183467836812 1106 40-41 6.653561916939843 1106 42-43 5.982502979819582 1106 44-45 4.037266292100803 1106 46-47 5.718408216319322 1106 48-49 5.596186195741911 1106 50-51 6.367634738628979 1106 52-53 5.390504597903973 1106 54-55 5.016055029488545 1106 56-57 4.4621319911637265 1106 58-59 4.825716558734108 1106 60-61 4.668058115777878 1106 62-63 4.798022098190852 1106 64-65 4.949304922815561 1106 66-67 2.666867288448689 1106 68-69 2.757827204966482 1106 70-71 2.46318198589179 1106 72-73 2.5672629384610843 1106 74-75 2.546352399277982 1106 76-77 3.3320884309536964 1106 78-79 4.146478006244472 1106 80-81 3.4643645375567473 1106 82-83 1.9805583202162609 1106 84-85 2.6560297677141733 1106 86-87 3.123861726468707 1106 88-89 3.1298484855896476 1106 90-91 2.9942939336795646 1106 92-93 2.6932935893402714 1106 94-95 1.1830014959505242 1106 96-97 1.0144757545151712 1106 98-99 1.8201000426514646 1106 100 2.640286743178926 1107 1 6.000085893569622 1107 2 10.522789975736469 1107 3 6.186448648776825 1107 4 11.43681874297528 1107 5 9.431729386965806 1107 6 8.088609973585022 1107 7 3.937737750640153 1107 8 6.608148462002756 1107 9 5.9046901704210235 1107 10-11 4.676678445767649 1107 12-13 6.465700420675553 1107 14-15 6.311051240142959 1107 16-17 7.191067002627502 1107 18-19 6.502026804184457 1107 20-21 5.4346055911834235 1107 22-23 5.838130285775506 1107 24-25 4.809467798650314 1107 26-27 7.469458057719958 1107 28-29 6.469229974042726 1107 30-31 5.0225561760066135 1107 32-33 4.921963537921318 1107 34-35 6.695981249722852 1107 36-37 6.679619279838981 1107 38-39 6.611840590966576 1107 40-41 7.761019938445465 1107 42-43 6.222977191985297 1107 44-45 4.181154664121252 1107 46-47 3.712461011017229 1107 48-49 4.168642179030481 1107 50-51 4.995732682272763 1107 52-53 3.904507929267357 1107 54-55 5.130937232245305 1107 56-57 5.028395110357486 1107 58-59 2.807231880470084 1107 60-61 2.7999332949751867 1107 62-63 0.6806065574196829 1107 64-65 1.944150769241272 1107 66-67 3.9452111834522263 1107 68-69 4.853190407444327 1107 70-71 4.057041198730413 1107 72-73 2.1944115020544324 1107 74-75 2.5355431198315976 1107 76-77 3.1279088181954293 1107 78-79 3.9912256476665036 1107 80-81 2.2176893669414 1107 82-83 1.6252939663895845 1107 84-85 2.38686847940496 1107 86-87 2.337675376866695 1107 88-89 2.554921578588907 1107 90-91 1.386173540391443 1107 92-93 2.0857705204290298 1107 94-95 2.287112550498673 1107 96-97 2.352487527412933 1107 98-99 1.9148820119397243 1107 100 0.3212581827843124 1108 1 4.366849921963432 1108 2 -3.0324206366018664 1108 3 0.07873014659302413 1108 4 6.6709138974597835 1108 5 6.770322938431033 1108 6 6.604735935174993 1108 7 3.946939647825708 1108 8 5.020842008419404 1108 9 4.5951477063073405 1108 10-11 3.2081770327444055 1108 12-13 2.4213944438165313 1108 14-15 3.906673199676497 1108 16-17 4.565007909621553 1108 18-19 3.7239482421731083 1108 20-21 4.531501352730309 1108 22-23 3.969567364908272 1108 24-25 2.6929033046313364 1108 26-27 4.505543526645969 1108 28-29 5.9228282599933095 1108 30-31 4.378390229693405 1108 32-33 4.335542223063975 1108 34-35 2.864433930497526 1108 36-37 0.918185892718391 1108 38-39 2.199436085170152 1108 40-41 5.192536291785679 1108 42-43 2.695581440265798 1108 44-45 3.540865330606529 1108 46-47 4.363645152630177 1108 48-49 4.62555913303564 1108 50-51 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2213 44-45 -17.321791461579398 2213 46-47 -17.12550234220948 2213 48-49 -6.053555560316891 2213 50-51 -23.545001426948364 2213 52-53 -18.012029802162502 2213 54-55 -17.876756466716348 2213 56-57 -17.677118231505133 2213 58-59 -18.08282379821121 2213 60-61 -19.27677427884068 2213 62-63 -17.47520518504134 2213 64-65 -17.61472399427372 2213 66-67 -8.693088930828853 2213 68-69 -22.701867784140273 2213 70-71 -17.292064559819597 2213 72-73 -9.32122371828611 2213 74-75 -4.045225913943742 2213 76-77 -2.851206341067151 2213 78-79 -4.176280698614772 2213 80-81 -3.0815979502062056 2213 82-83 -3.125770149457928 2213 84-85 -2.9580795179018082 2213 86-87 -3.247667300981238 2213 88-89 -2.6503455414318395 2213 90-91 -2.3893230914644867 2213 92-93 -3.5994576629286144 2213 94-95 -3.5124508658974065 2213 96-97 -3.30379451743946 2213 98-99 -3.7080523121853197 2213 100 -3.0043228527452577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 271.0 3 244.0 4 1253.0 5 4665.0 6 7785.0 7 8287.0 8 7666.0 9 7768.0 10 9217.0 11 11222.0 12 14073.0 13 17874.0 14 14613.0 15 16582.0 16 18728.0 17 19862.0 18 19882.0 19 20505.0 20 21007.0 21 20909.0 22 22350.0 23 24498.0 24 26691.0 25 29059.0 26 31110.0 27 33260.0 28 36330.0 29 39069.0 30 41632.0 31 46484.0 32 53873.0 33 63386.0 34 74610.0 35 82649.0 36 82886.0 37 64237.0 38 17544.0 39 29.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.520759977151236 20.373338562403553 13.484713495263998 23.62118796518121 2 14.800794275808116 22.43894134678612 37.951361003033114 24.808903374372647 3 18.881339134232586 28.019540239539065 28.932929902067826 24.166190724160522 4 13.14360796047332 19.545954066799283 35.25899865571851 32.05143931700889 5 13.15535545562954 39.03313213027963 34.12918418501608 13.682328229074752 6 30.816694111684185 35.423323986800284 18.60358880765357 15.156393093861961 7 25.291245502826794 33.31655816344012 22.675496830563645 18.716699503169437 8 25.414475099073087 34.801643094819454 21.821150793179058 17.9627310129284 9 25.674566394706545 17.960486120058686 20.97130495139215 35.393642533842616 10-11 23.03763118195023 28.90293897273171 26.90861905137082 21.15081079394724 12-13 24.816627052173683 26.73778883936546 26.696435351168684 21.749148757292172 14-15 23.43600117111602 27.380905485613905 24.681855686566767 24.50123765670331 16-17 21.423373412418545 30.50038524900025 27.109659350054727 20.966581988526478 18-19 22.31594954163851 29.47810555902759 27.99891291819075 20.20703198114315 20-21 22.983831976069848 28.82741089903146 27.09547751579912 21.093279609099568 22-23 21.86279017820945 29.141145212952107 25.938532987868452 23.05753162096999 24-25 22.266374563251222 29.400458781541232 25.233088941531395 23.10007771367615 26-27 22.963271710881184 27.80474781651656 25.952532731819744 23.279447740782512 28-29 22.6734802808405 27.60498092882868 27.2860928365459 22.435445953784914 30-31 23.66780433951236 28.60905320948471 26.256791762575133 21.466350688427795 32-33 21.36780216104701 28.623957611187688 27.139257687862333 22.868982539902966 34-35 20.820934371959837 28.554549869377304 27.283328646486215 23.34118711217664 36-37 23.12970886650658 28.285954070990048 26.262867859112088 22.321469203391285 38-39 23.612254818763372 27.409015000555264 26.17197731163397 22.806752869047394 40-41 22.40071043396388 28.490916288624497 26.061585209278437 23.046788068133182 42-43 23.46663575188782 27.959677291865376 26.26993871496398 22.303748241282822 44-45 24.05776518887302 27.248684345486534 26.142456256624296 22.551094209016146 46-47 22.509767236245338 27.870367993949262 27.868272863503822 21.751591906301577 48-49 23.119867962740027 27.543064835325616 27.652668081840513 21.684399120093843 50-51 22.29259331903415 27.911138387819967 27.34829263971756 22.447975653428323 52-53 21.528582222403553 28.21280479018908 26.117406939349507 24.14120604805786 54-55 22.185907427531063 28.65384266259314 25.484531055892568 23.67571885398323 56-57 22.983344211019528 27.25879204932505 25.86022500778332 23.897638731872103 58-59 22.817333294440527 26.627855742496887 27.08730257424304 23.467508388819546 60-61 23.49160892645934 27.651322920709166 26.361805920846077 22.495262231985418 62-63 21.4647123711109 27.8530445048436 27.1520937278898 23.530149396155707 64-65 20.870434111394818 27.954930369197733 27.28073556415055 23.8938999552569 66-67 22.746604555797823 27.44534980627461 26.332080287372406 23.475965350555168 68-69 23.711335919168903 26.71957891979162 25.975692036756154 23.593393124283324 70-71 22.338387933748592 27.698714230324974 26.008819483039808 23.95407835288663 72-73 23.36218637237971 27.14928564408367 26.225226201006496 23.26330178253012 74-75 23.769710443093896 26.561939213409147 25.902024842488192 23.76632550100876 76-77 22.455530586766542 27.313707865168542 27.3478152309613 22.88294631710362 78-79 23.023990082906977 27.05928858519689 27.171046749570294 22.745674582325837 80-81 22.248543928276742 27.11521274685636 27.063264223304007 23.572979101562893 82-83 21.60504946858961 27.511920531778628 26.245174311963126 24.63785568766863 84-85 22.117645058516484 27.644743468122357 25.464958871186727 24.772652602174432 86-87 22.80113468054733 26.574339488202902 25.967353869192195 24.65717196205757 88-89 22.686949851204247 26.069603181473113 27.11843664464828 24.125010322674363 90-91 23.195855598079437 26.67037009322823 26.448263609968727 23.685510698723604 92-93 21.640244485477172 26.99159698837173 26.970442068465644 24.397716457685455 94-95 21.069319790986025 27.117994387367006 27.105283541241732 24.707402280405237 96-97 22.7356396970588 26.634358634062604 26.487437715066797 24.1425639538118 98-99 23.058370829423268 26.051772146182394 26.29448974963513 24.595367274759205 100 22.088417143888616 26.57324555226498 26.837164002356523 24.501173301489885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 845.0 1 914.5 2 860.5 3 660.5 4 513.5 5 423.0 6 429.0 7 479.0 8 591.5 9 771.5 10 950.5 11 1181.5 12 1499.0 13 1823.5 14 2172.5 15 2459.0 16 2775.0 17 3148.0 18 3453.0 19 3652.0 20 3934.0 21 4312.5 22 4711.0 23 5292.0 24 6076.0 25 7128.5 26 8303.5 27 9677.0 28 11560.5 29 13622.5 30 15488.5 31 17438.0 32 19226.5 33 20967.5 34 22638.0 35 24181.5 36 25725.5 37 27093.0 38 28379.5 39 29364.0 40 30023.5 41 30247.0 42 29921.0 43 29460.0 44 29101.5 45 29078.5 46 29782.0 47 31450.0 48 34481.0 49 40483.5 50 49573.5 51 49511.5 52 38648.0 53 29885.0 54 25332.0 55 22480.0 56 20211.0 57 18338.0 58 16508.0 59 14964.5 60 13456.5 61 11753.0 62 10055.0 63 8312.0 64 6858.0 65 5677.5 66 4717.5 67 3893.0 68 3117.0 69 2451.5 70 1915.0 71 1467.5 72 1144.5 73 933.0 74 751.5 75 570.0 76 398.0 77 278.0 78 188.5 79 125.5 80 97.0 81 60.5 82 30.0 83 22.0 84 18.0 85 14.5 86 8.0 87 5.0 88 6.0 89 3.5 90 2.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.2883382241060755 2 23.970121824702847 3 5.890071237316102 4 21.870448864253884 5 11.428500854650187 6 9.158688284870221 7 7.725444862712551 8 8.574463250041992 9 4.911916688897452 10-11 6.572852753159242 12-13 9.567191313197181 14-15 7.196352175158827 16-17 7.607918111667704 18-19 6.56638112458132 20-21 7.530752586181344 22-23 5.5622906601061155 24-25 5.155170880635504 26-27 9.329519518629398 28-29 6.1502208257995665 30-31 4.149845372538558 32-33 8.57332700990999 34-35 6.716513027240121 36-37 6.641274169803678 38-39 5.692266650858109 40-41 7.654306350100286 42-43 6.250802778354131 44-45 6.0801197498295645 46-47 5.68273211409827 48-49 3.768612107379633 50-51 6.304057859323592 52-53 4.343846024641591 54-55 4.7586230745669935 56-57 4.475699281698629 58-59 3.4645937694519366 60-61 4.0174486962879525 62-63 3.0854353775775363 64-65 4.3832192153026845 66-67 2.3697522996512235 68-69 4.960231595379948 70-71 2.4006283901947416 72-73 1.4808667042119927 74-75 0.756982936637322 76-77 1.0730058985683375 78-79 1.6457697285868138 80-81 2.0495795911511596 82-83 0.7243283832784975 84-85 1.2116765964173855 86-87 0.43725484384108443 88-89 0.6956753712541127 90-91 0.3096995385877029 92-93 0.2853938801118455 94-95 0.5035025837112567 96-97 0.5380838051199969 98-99 0.4505933149558842 100 0.3798006145577062 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1012110.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.71480711555584 #Duplication Level Percentage of deduplicated Percentage of total 1 96.18387428836182 85.3293385511889 2 2.7835328847377987 4.9388116593864115 3 0.5551196972743992 1.4774201060923224 4 0.18682404241748568 0.6629623555046267 5 0.07687418417004514 0.34099392104056353 6 0.04660420316881115 0.24806897369371544 7 0.03333718497991865 0.2070251354688362 8 0.020604687706260515 0.1462352716429733 9 0.016564884907524398 0.13225955125161679 >10 0.07921778623552246 1.33083095809779 >50 0.008895773300896314 0.5487490004118076 >100 0.005512749823790186 0.9441894644908599 >500 0.0018000787648734132 1.0560740057929423 >1k 0.001012544305241295 1.48944262247879 >5k 2.2500984560917665E-4 1.1475984234578254 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6306 0.6230548062957584 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5192 0.5129877187262254 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2243 0.22161622748515475 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2173 0.21469998320340677 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2110 0.20847536334983352 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1562 0.15433105097272035 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1543 0.15245378466767445 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1485 0.1467231822627975 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1346 0.1329894971890407 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1306 0.12903735759947044 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1155 0.11411803064884252 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.88034897392576E-5 0.0 4 0.0 0.0 0.0 9.88034897392576E-5 0.0 5 0.0 0.0 0.0 9.88034897392576E-5 0.0 6 0.0 0.0 0.0 9.88034897392576E-5 0.0 7 0.0 0.0 0.0 9.88034897392576E-5 0.0 8 0.0 0.0 0.0 9.88034897392576E-5 0.0 9 0.0 0.0 0.0 1.976069794785152E-4 0.0 10-11 0.0 0.0 0.0 1.976069794785152E-4 0.0 12-13 0.0 0.0 0.0 2.9641046921777275E-4 0.0 14-15 0.0 0.0 0.0 2.9641046921777275E-4 0.0 16-17 0.0 4.94017448696288E-5 0.0 2.9641046921777275E-4 0.0 18-19 0.0 9.88034897392576E-5 0.0 2.9641046921777275E-4 0.0 20-21 0.0 9.88034897392576E-5 0.0 3.458122140874016E-4 0.0 22-23 0.0 9.88034897392576E-5 0.0 4.940174486962879E-4 0.0 24-25 0.0 9.88034897392576E-5 0.0 0.0012844453666103488 0.0 26-27 0.0 9.88034897392576E-5 0.0 0.0022230785191332957 0.0 28-29 0.0 9.88034897392576E-5 0.0 0.003853336099831046 0.0 30-31 0.0 9.88034897392576E-5 0.0 0.009781545484186503 0.0 32-33 0.0 9.88034897392576E-5 0.0 0.02544189860785883 0.0 34-35 0.0 9.88034897392576E-5 0.0 0.049451146614498426 0.0 36-37 0.0 9.88034897392576E-5 0.0 0.08418057325784747 0.0 38-39 0.0 9.88034897392576E-5 0.0 0.1367440297991325 0.0 40-41 0.0 9.88034897392576E-5 0.0 0.217861694875063 4.94017448696288E-5 42-43 0.0 9.88034897392576E-5 0.0 0.28055250911462193 9.88034897392576E-5 44-45 0.0 9.88034897392576E-5 0.0 0.3507029868294948 9.88034897392576E-5 46-47 0.0 9.88034897392576E-5 0.0 0.44036715376787106 9.88034897392576E-5 48-49 0.0 9.88034897392576E-5 0.0 0.5238067008526741 1.482052346088864E-4 50-51 0.0 9.88034897392576E-5 0.0 0.6171265969114029 1.976069794785152E-4 52-53 0.0 9.88034897392576E-5 0.0 0.7269466757565877 1.976069794785152E-4 54-55 0.0 9.88034897392576E-5 0.0 0.8404718854669947 1.976069794785152E-4 56-57 0.0 1.976069794785152E-4 0.0 0.9659029156909822 1.976069794785152E-4 58-59 0.0 1.976069794785152E-4 0.0 1.106450879845076 1.976069794785152E-4 60-61 0.0 1.976069794785152E-4 0.0 1.256730987738487 2.9641046921777275E-4 62-63 0.0 1.976069794785152E-4 0.0 1.404442204898677 2.9641046921777275E-4 64-65 0.0 1.976069794785152E-4 0.0 1.5810040410627302 2.9641046921777275E-4 66-67 0.0 1.976069794785152E-4 0.0 1.7827113653654247 2.9641046921777275E-4 68-69 0.0 1.976069794785152E-4 0.0 2.0132693086719824 2.9641046921777275E-4 70-71 0.0 1.976069794785152E-4 0.0 2.3376905672308346 2.9641046921777275E-4 72-73 0.0 1.976069794785152E-4 0.0 2.6279258183399037 2.9641046921777275E-4 74-75 0.0 1.976069794785152E-4 0.0 2.9223602177628916 2.9641046921777275E-4 76-77 0.0 1.976069794785152E-4 0.0 3.254438746776536 2.9641046921777275E-4 78-79 0.0 1.976069794785152E-4 0.0 3.6101313098378633 2.9641046921777275E-4 80-81 9.88034897392576E-5 1.976069794785152E-4 0.0 3.9968975704221874 2.9641046921777275E-4 82-83 9.88034897392576E-5 1.976069794785152E-4 0.0 4.476341504381935 2.9641046921777275E-4 84-85 9.88034897392576E-5 1.976069794785152E-4 0.0 4.936419954352788 2.9641046921777275E-4 86-87 9.88034897392576E-5 1.976069794785152E-4 0.0 5.408799438796178 2.9641046921777275E-4 88 9.88034897392576E-5 1.976069794785152E-4 0.0 5.8211064014781 2.9641046921777275E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTGCG 245 0.0 32.577766 9 GATACGA 140 3.5109953E-4 21.355553 6 TGATACG 145 4.605315E-4 20.514254 5 TACTGTG 450 0.0 19.889088 7 GTACATA 555 0.0 19.595148 1 ATACGAC 155 7.075328E-4 19.247503 7 CCTATAC 150 0.007662073 18.227224 3 TACATCG 235 1.5097317E-4 17.989359 2 GTCTAAC 170 0.008869042 17.770111 1 GTATAGA 210 0.0014689418 17.262392 1 ACGACTG 175 0.0015632443 17.103325 9 GTGTATA 215 0.001719536 16.86094 1 TGTGCGT 225 6.366463E-11 16.635008 10-11 GTGCGTT 210 4.274625E-10 16.635008 10-11 GTACTGT 490 1.2732926E-11 16.270899 6 GTACATT 690 0.0 15.761315 1 AGTACTC 410 6.0608727E-9 15.719255 5 TACGACT 195 0.0032299794 15.341999 8 GTCCGTG 200 0.003810972 14.96541 9 TGTACTG 500 3.45608E-10 14.872833 5 >>END_MODULE