##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295118_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012110 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52369406487437 33.0 33.0 33.0 27.0 33.0 2 30.15457707166217 33.0 33.0 33.0 27.0 33.0 3 30.973840788056634 33.0 33.0 33.0 27.0 33.0 4 35.27613006491389 37.0 37.0 37.0 33.0 37.0 5 35.59466263548428 37.0 37.0 37.0 33.0 37.0 6 35.39868492555157 37.0 37.0 37.0 33.0 37.0 7 35.2918526642361 37.0 37.0 37.0 33.0 37.0 8 35.29494224936025 37.0 37.0 37.0 33.0 37.0 9 35.24255663910049 37.0 37.0 37.0 33.0 37.0 10-11 35.2229629190503 37.0 37.0 37.0 33.0 37.0 12-13 35.140201163905104 37.0 37.0 37.0 33.0 37.0 14-15 36.55135953601881 38.5 37.0 40.0 33.0 40.0 16-17 36.50899753979311 37.0 37.0 40.0 33.0 40.0 18-19 36.56169339301064 38.5 37.0 40.0 33.0 40.0 20-21 36.583373348746676 40.0 37.0 40.0 33.0 40.0 22-23 36.574578850124986 40.0 37.0 40.0 33.0 40.0 24-25 36.53409659029157 40.0 37.0 40.0 33.0 40.0 26-27 36.451878748357394 37.0 37.0 40.0 33.0 40.0 28-29 36.377505409491064 37.0 37.0 40.0 33.0 40.0 30-31 36.21528242977542 37.0 37.0 40.0 33.0 40.0 32-33 36.12523391726196 37.0 37.0 40.0 27.0 40.0 34-35 36.05269931133968 37.0 37.0 40.0 27.0 40.0 36-37 36.017459564671825 37.0 37.0 40.0 27.0 40.0 38-39 35.92366244775765 37.0 37.0 40.0 27.0 40.0 40-41 35.80798431000583 37.0 37.0 40.0 27.0 40.0 42-43 35.64526830087638 37.0 37.0 40.0 27.0 40.0 44-45 35.45644149351355 37.0 37.0 40.0 27.0 40.0 46-47 35.22648032328502 37.0 33.0 40.0 27.0 40.0 48-49 35.024475106460756 37.0 33.0 40.0 24.5 40.0 50-51 34.9059692128326 37.0 33.0 40.0 22.0 40.0 52-53 34.83714517196748 37.0 33.0 40.0 22.0 40.0 54-55 34.6924978510241 37.0 33.0 40.0 22.0 40.0 56-57 34.51186975723983 37.0 33.0 40.0 22.0 40.0 58-59 34.38933959747458 37.0 33.0 40.0 22.0 40.0 60-61 34.17816739287231 37.0 33.0 40.0 22.0 40.0 62-63 33.96756133226626 37.0 33.0 40.0 22.0 40.0 64-65 33.81557241801781 37.0 33.0 38.5 22.0 40.0 66-67 33.55949501536394 37.0 33.0 37.0 22.0 40.0 68-69 33.33121844463547 37.0 33.0 37.0 22.0 40.0 70-71 33.03782691604667 37.0 33.0 37.0 22.0 40.0 72-73 32.779750718795384 37.0 33.0 37.0 22.0 40.0 74-75 32.55705950934187 37.0 33.0 37.0 22.0 37.0 76-77 32.292480560413395 37.0 33.0 37.0 22.0 37.0 78-79 32.07145863591902 35.0 33.0 37.0 22.0 37.0 80-81 31.926242206874747 33.0 33.0 37.0 22.0 37.0 82-83 31.7785324717669 33.0 33.0 37.0 22.0 37.0 84-85 31.57505854106767 33.0 33.0 37.0 22.0 37.0 86-87 31.59343648417662 33.0 33.0 37.0 22.0 37.0 88-89 31.636185790082106 33.0 33.0 37.0 22.0 37.0 90-91 31.678815049747556 33.0 33.0 37.0 22.0 37.0 92-93 31.626478347215222 33.0 33.0 37.0 22.0 37.0 94-95 31.714543873689617 33.0 33.0 37.0 22.0 37.0 96-97 31.689339103457137 33.0 33.0 37.0 22.0 37.0 98-99 31.602283842665322 33.0 33.0 37.0 22.0 37.0 100 31.01733210817006 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6326380389933703 1101 2 4.0774604491520705 1101 3 2.3013311357189963 1101 4 1.0845802959969646 1101 5 0.3902206313845298 1101 6 0.37192488439646354 1101 7 0.4447183824472418 1101 8 0.5328686466941903 1101 9 0.5664704453855762 1101 10-11 0.5420669381424119 1101 12-13 0.5977813645177932 1101 14-15 0.9222063315424762 1101 16-17 0.8750431886036125 1101 18-19 0.8727272451099068 1101 20-21 0.7965315733500233 1101 22-23 0.8550098488899422 1101 24-25 0.767485067674393 1101 26-27 0.7989264840325845 1101 28-29 0.7420034373509736 1101 30-31 0.6673832730522946 1101 32-33 0.8633336850224822 1101 34-35 1.1061291697440012 1101 36-37 0.9083938791670576 1101 38-39 0.8042687206740382 1101 40-41 0.9782719858788411 1101 42-43 0.9052454356988662 1101 44-45 0.9990261751680336 1101 46-47 0.9615406659733026 1101 48-49 0.7423831476615987 1101 50-51 0.6895968962702028 1101 52-53 0.67054064616147 1101 54-55 0.6566402018077042 1101 56-57 0.5056056825917707 1101 58-59 0.671352206247569 1101 60-61 0.6847964718551154 1101 62-63 0.7604959133995521 1101 64-65 0.5975137674035338 1101 66-67 0.7375766010714244 1101 68-69 0.7297733711634251 1101 70-71 0.7751352595304795 1101 72-73 0.7582069447073607 1101 74-75 0.7217440638079751 1101 76-77 0.6540342573691049 1101 78-79 0.795249075535601 1101 80-81 0.8590305639104159 1101 82-83 0.582157523621774 1101 84-85 0.7726575497274908 1101 86-87 0.825286879044576 1101 88-89 0.7749461097572699 1101 90-91 0.7473963444992187 1101 92-93 0.6937918588131105 1101 94-95 0.6589846051812174 1101 96-97 0.6602747078075311 1101 98-99 0.6086871898981094 1101 100 0.6941613312264714 1102 1 0.5437950339460436 1102 2 4.021157499254134 1102 3 2.228740151506223 1102 4 0.9826625219001457 1102 5 0.4754482206365296 1102 6 0.403213283535365 1102 7 0.3778458512228937 1102 8 0.39055706519033606 1102 9 0.5468723595770797 1102 10-11 0.53599107011906 1102 12-13 0.46015631849275707 1102 14-15 0.8999926729423962 1102 16-17 0.9102578073756007 1102 18-19 0.870586600089716 1102 20-21 0.7629595069059008 1102 22-23 0.8464415479177845 1102 24-25 0.8521228424014495 1102 26-27 0.9053732346319165 1102 28-29 0.9395029576401654 1102 30-31 0.7617094242597631 1102 32-33 0.9453452892410894 1102 34-35 0.900056846797618 1102 36-37 0.8408349347596911 1102 38-39 0.8814630529775442 1102 40-41 0.8727374829952268 1102 42-43 0.869094185083874 1102 44-45 0.7807301829994628 1102 46-47 0.8356677376628241 1102 48-49 0.884196824043336 1102 50-51 0.8277993771863379 1102 52-53 0.8831066497737012 1102 54-55 0.8283461922562623 1102 56-57 0.7533148407260271 1102 58-59 0.7070109912765403 1102 60-61 0.7022713566729308 1102 62-63 0.8179801804792817 1102 64-65 0.8505547963236637 1102 66-67 0.7557842289141234 1102 68-69 0.7196158815881049 1102 70-71 0.7979140671729184 1102 72-73 0.7799061920068695 1102 74-75 0.8548925592041599 1102 76-77 0.8005510691932329 1102 78-79 0.7643187479273408 1102 80-81 0.7536565273413558 1102 82-83 0.7942430661414122 1102 84-85 0.765019690781422 1102 86-87 0.8079771497965424 1102 88-89 0.8329189034486113 1102 90-91 0.8204314949689824 1102 92-93 0.8410689864829628 1102 94-95 0.6905611121624986 1102 96-97 0.8056660983724129 1102 98-99 0.8416080313305692 1102 100 0.6735985718285953 1103 1 0.4100308933843806 1103 2 3.8946673471784337 1103 3 2.1235288713798077 1103 4 0.8782770180233967 1103 5 0.3616188289031612 1103 6 0.3225436243022699 1103 7 0.3377621999745912 1103 8 0.3530798059222917 1103 9 0.30815220997033776 1103 10-11 0.33354206675180365 1103 12-13 0.22827908998668534 1103 14-15 0.5893495630439105 1103 16-17 0.5582561185127872 1103 18-19 0.5404473279187769 1103 20-21 0.5374966067789586 1103 22-23 0.5049120648693233 1103 24-25 0.3880403972661739 1103 26-27 0.5092143124888437 1103 28-29 0.3593937145617474 1103 30-31 0.35142502638638007 1103 32-33 0.3788187139225059 1103 34-35 0.5389407810713109 1103 36-37 0.4620081766395998 1103 38-39 0.34119342123225493 1103 40-41 0.45914150915888996 1103 42-43 0.37947768372156787 1103 44-45 0.48099891244429216 1103 46-47 0.37123998175832185 1103 48-49 0.30021663582041214 1103 50-51 0.34542945265025793 1103 52-53 0.29987637983928295 1103 54-55 0.4572686532369943 1103 56-57 0.4322852816292482 1103 58-59 0.3508974582733799 1103 60-61 0.35014688641753366 1103 62-63 0.40459674218530495 1103 64-65 0.32284532032228697 1103 66-67 0.3263387953444692 1103 68-69 0.44236483704205654 1103 70-71 0.38335525878822097 1103 72-73 0.38003080084343566 1103 74-75 0.43416993455296904 1103 76-77 0.33027806238733604 1103 78-79 0.3684964752304438 1103 80-81 0.3638312214681605 1103 82-83 0.3295058820917234 1103 84-85 0.32533752516892633 1103 86-87 0.4746117727405945 1103 88-89 0.4962432829409593 1103 90-91 0.3710280888037545 1103 92-93 0.48467600683745005 1103 94-95 0.3907303791613863 1103 96-97 0.43242484664181546 1103 98-99 0.3945721169166241 1103 100 0.4487176935742454 1104 1 0.3368240974096395 1104 2 3.456746008039712 1104 3 2.168147675192852 1104 4 0.996401335920865 1104 5 0.35935522256876595 1104 6 0.272166330342003 1104 7 0.2909714911704313 1104 8 0.2774478439504051 1104 9 0.3274090984925948 1104 10-11 0.25696022841930244 1104 12-13 0.3672111510983527 1104 14-15 0.43327082661758 1104 16-17 0.4436921151076376 1104 18-19 0.3546262643957192 1104 20-21 0.39729686314915114 1104 22-23 0.35585448311639567 1104 24-25 0.3693712041553425 1104 26-27 0.4742904659284122 1104 28-29 0.43631943293157605 1104 30-31 0.30968281106297013 1104 32-33 0.5803150988036734 1104 34-35 0.5190742557217973 1104 36-37 0.46163782915447626 1104 38-39 0.3426579861408712 1104 40-41 0.30862634832930524 1104 42-43 0.3551891267504388 1104 44-45 0.42895672746496416 1104 46-47 0.4530857920498903 1104 48-49 0.239520776116386 1104 50-51 0.15358689716602925 1104 52-53 0.145547151551348 1104 54-55 0.2674067286973596 1104 56-57 0.13812154081266215 1104 58-59 0.1899149629971859 1104 60-61 0.19357874818701504 1104 62-63 0.41448808986838515 1104 64-65 0.3716903556784743 1104 66-67 0.1971376045651425 1104 68-69 0.13296037232799307 1104 70-71 0.22974576797469126 1104 72-73 0.20886089635845195 1104 74-75 0.27784718596853963 1104 76-77 0.26539906683583325 1104 78-79 0.15345397185460996 1104 80-81 0.30620893103623104 1104 82-83 0.32725181978125306 1104 84-85 0.31727066032765094 1104 86-87 0.34665868447156 1104 88-89 0.19179442966035154 1104 90-91 0.1831038962970517 1104 92-93 0.17195081214920194 1104 94-95 0.15293885842645238 1104 96-97 0.1496037354970312 1104 98-99 0.16585037067116204 1104 100 0.04678972202763987 1105 1 0.43774639539357807 1105 2 3.691565130754075 1105 3 2.042521555475389 1105 4 0.9313029341887997 1105 5 0.17536175783432384 1105 6 0.07538091509603362 1105 7 0.14227418900778588 1105 8 0.18069954662720988 1105 9 0.2442629896358497 1105 10-11 0.16341031109104165 1105 12-13 0.32055694339336327 1105 14-15 0.36451654491216345 1105 16-17 0.3922070843029033 1105 18-19 0.37906151088462536 1105 20-21 0.3570135192088273 1105 22-23 0.3959518450757855 1105 24-25 0.3335932148481291 1105 26-27 0.29680538960909786 1105 28-29 0.3722566840237249 1105 30-31 0.21672419087661865 1105 32-33 0.23822066704140354 1105 34-35 0.48193926497773276 1105 36-37 0.3858815612870643 1105 38-39 0.40490289851353367 1105 40-41 0.28920731837050795 1105 42-43 0.21513782868253628 1105 44-45 0.3179378444083909 1105 46-47 0.5417950771021935 1105 48-49 0.3859758967810265 1105 50-51 0.21014287970412227 1105 52-53 0.35544566973795355 1105 54-55 0.3200653293438336 1105 56-57 0.3440914839272864 1105 58-59 0.5107252480361453 1105 60-61 0.44813334488468826 1105 62-63 0.4597315418508998 1105 64-65 0.45737194040189166 1105 66-67 0.3762757232593117 1105 68-69 0.3439306411072707 1105 70-71 0.392256352075826 1105 72-73 0.42138199589647485 1105 74-75 0.40619692155237885 1105 76-77 0.3952309689449649 1105 78-79 0.42126135416216215 1105 80-81 0.43041801715844485 1105 82-83 0.4250599005021414 1105 84-85 0.3957014981804008 1105 86-87 0.41454995485404567 1105 88-89 0.4197789107588541 1105 90-91 0.3665580525234873 1105 92-93 0.2729472675052875 1105 94-95 0.289094736437967 1105 96-97 0.29776251388576824 1105 98-99 0.4334295915400652 1105 100 0.45276562912139795 1106 1 0.5785733703761515 1106 2 3.895642642801228 1106 3 2.245244012277542 1106 4 0.9227296550272825 1106 5 0.29842618848888236 1106 6 0.21598090787819046 1106 7 0.28375684813750013 1106 8 0.27119231643687414 1106 9 0.3020834286707128 1106 10-11 0.25960755197518637 1106 12-13 0.2406963427547666 1106 14-15 0.27468192851208784 1106 16-17 0.15443838581313685 1106 18-19 0.2589501281546518 1106 20-21 0.30094350232286615 1106 22-23 0.2500948875490039 1106 24-25 0.14834449364115443 1106 26-27 0.2632368115433579 1106 28-29 0.21931037908501594 1106 30-31 0.2584571002565923 1106 32-33 0.31269637614708756 1106 34-35 0.2506997712145207 1106 36-37 0.3123925124659479 1106 38-39 0.28916704881184074 1106 40-41 0.3509166557914156 1106 42-43 0.31268870023719586 1106 44-45 0.12447873064567716 1106 46-47 0.06924976138800076 1106 48-49 0.02249201441668447 1106 50-51 0.14307780704132256 1106 52-53 0.17898049488567835 1106 54-55 0.17068568404387463 1106 56-57 0.35229570332695914 1106 58-59 0.004533287574737699 1106 60-61 0.1536170069148426 1106 62-63 0.041343324411450055 1106 64-65 0.1250757922613417 1106 66-67 0.11232961396216012 1106 68-69 0.03542650129382707 1106 70-71 0.09542204148439026 1106 72-73 0.05798767178108477 1106 74-75 -0.08608283448448617 1106 76-77 0.05383957755357471 1106 78-79 0.05031042220553417 1106 80-81 0.014438185334213927 1106 82-83 4.431585229696111E-4 1106 84-85 0.20736800339529537 1106 86-87 0.24036001538896556 1106 88-89 0.16320411964895953 1106 90-91 0.2683804631694251 1106 92-93 0.17504974949391894 1106 94-95 0.11228547936305588 1106 96-97 0.20373993343054764 1106 98-99 0.2156497623750404 1106 100 0.3371887619316283 1107 1 0.34335317466830517 1107 2 3.487682673774323 1107 3 2.093156156345355 1107 4 0.884543999784384 1107 5 0.33472966242993607 1107 6 0.07913247906072485 1107 7 0.07729560254307444 1107 8 0.015142517625470475 1107 9 0.05049644751397864 1107 10-11 -0.008863668204909914 1107 12-13 0.011830313104077561 1107 14-15 0.09066993716426452 1107 16-17 1.9713893141926064E-4 1107 18-19 0.11757422436629383 1107 20-21 0.15036236665562797 1107 22-23 0.14080549388443586 1107 24-25 0.013874732395365186 1107 26-27 0.06846752016024737 1107 28-29 0.013575479932875112 1107 30-31 -0.039820850135740216 1107 32-33 0.13132489987330587 1107 34-35 0.18009427446494186 1107 36-37 0.12533636104885204 1107 38-39 0.13378674091428167 1107 40-41 0.13483141735903104 1107 42-43 0.0861069365260434 1107 44-45 0.08262896577205936 1107 46-47 0.11564913880533112 1107 48-49 0.001790848244461074 1107 50-51 0.10690228241985977 1107 52-53 -0.022238046843156667 1107 54-55 -0.10469870028779127 1107 56-57 0.09108297393001408 1107 58-59 0.012575272895674061 1107 60-61 0.12982526003604278 1107 62-63 -0.026813873810525024 1107 64-65 -0.11998723643168319 1107 66-67 -0.010528551486451931 1107 68-69 0.059248480100272616 1107 70-71 0.13356546955093762 1107 72-73 -0.005028733428169119 1107 74-75 0.041345715767796776 1107 76-77 -0.015648800708305544 1107 78-79 0.10879194074550469 1107 80-81 0.011627883853627452 1107 82-83 0.08138280584315538 1107 84-85 0.07230052768323603 1107 86-87 -0.08188931804578914 1107 88-89 0.06921881041534661 1107 90-91 -0.053516229457688524 1107 92-93 0.06076652899394119 1107 94-95 0.0794415131682058 1107 96-97 -0.02210797461757963 1107 98-99 0.12693114750443968 1107 100 0.20698882161530463 1108 1 0.403195951986401 1108 2 3.560862941472184 1108 3 2.0718771785779744 1108 4 0.9194240729771792 1108 5 0.32755856464210353 1108 6 0.1363505748534095 1108 7 0.09034925503377877 1108 8 0.041115515065342834 1108 9 -0.0018673074088653152 1108 10-11 0.06199290560006432 1108 12-13 0.11990163753829819 1108 14-15 -0.07842055991238084 1108 16-17 -0.0904428201250127 1108 18-19 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0.10487526369563938 2211 78-79 0.06569622191548774 2211 80-81 0.1643787525776581 2211 82-83 -0.4271258596280276 2211 84-85 -0.13324046221317332 2211 86-87 0.12470271227859797 2211 88-89 0.16687951415664415 2211 90-91 0.08926404118692233 2211 92-93 0.34282428247495744 2211 94-95 0.19991965833015257 2211 96-97 0.11206498922118868 2211 98-99 0.14771861828619492 2211 100 9.678906043752988E-4 2212 1 0.5800984248423795 2212 2 4.158714538226064 2212 3 2.3461154719725315 2212 4 1.2241457770316444 2212 5 0.6360078878352908 2212 6 0.6264384242180583 2212 7 0.694976891710489 2212 8 0.38197663016236305 2212 9 0.5551335376031687 2212 10-11 0.4907077698401139 2212 12-13 0.4793673667199059 2212 14-15 0.6653682030218917 2212 16-17 0.46952553178263656 2212 18-19 0.3817714789171731 2212 20-21 0.6253988400135029 2212 22-23 0.5088639507298254 2212 24-25 0.49148174854311577 2212 26-27 0.5661779880139477 2212 28-29 0.39382018300658217 2212 30-31 0.5511586253110607 2212 32-33 0.4803434349706208 2212 34-35 0.44933811544110114 2212 36-37 0.7516626649713984 2212 38-39 0.7633500553477859 2212 40-41 0.7843589215866231 2212 42-43 0.9101287873831652 2212 44-45 0.8931606478570089 2212 46-47 0.9512214389479112 2212 48-49 0.9950955002554664 2212 50-51 0.6115418593289021 2212 52-53 0.5687953097004979 2212 54-55 0.8382238104055304 2212 56-57 0.8894416728258676 2212 58-59 0.9122130261368326 2212 60-61 0.9229525188974108 2212 62-63 0.8763651109038264 2212 64-65 0.6441934393201691 2212 66-67 0.969028960367389 2212 68-69 0.8047075470454601 2212 70-71 0.7843653966042226 2212 72-73 1.036963706639476 2212 74-75 1.1565642243390357 2212 76-77 0.8223580960720938 2212 78-79 0.666650291486576 2212 80-81 0.885407684244889 2212 82-83 1.2000074286754767 2212 84-85 0.9444050404323292 2212 86-87 0.6412314750839556 2212 88-89 0.9130407209561433 2212 90-91 0.6610275219929527 2212 92-93 0.7861063943740909 2212 94-95 1.05814604581186 2212 96-97 0.8557551839752122 2212 98-99 0.8074253615035794 2212 100 0.5964480211908878 2213 1 -0.6742387490418729 2213 2 -25.950544721033197 2213 3 -14.891800035907222 2213 4 -6.285493122893314 2213 5 -2.4489808553775276 2213 6 -1.6957882124305073 2213 7 -1.7688490916029806 2213 8 -1.4697405816859614 2213 9 -1.7348258496744506 2213 10-11 -1.513266317477246 2213 12-13 -1.6257908904645646 2213 14-15 -1.544307115920411 2213 16-17 -1.9824267482685727 2213 18-19 -1.8492674563231901 2213 20-21 -1.8647956154670098 2213 22-23 -1.6699611496983735 2213 24-25 -1.5637118240521275 2213 26-27 -1.9057749542012132 2213 28-29 -1.922588239718877 2213 30-31 -1.8864633232167307 2213 32-33 -1.834067459833598 2213 34-35 -1.7693139394743653 2213 36-37 -2.1368013353817616 2213 38-39 -2.084319127541896 2213 40-41 -1.8554437535557398 2213 42-43 -1.58380027278546 2213 44-45 -1.84772466534654 2213 46-47 -1.6821240340578711 2213 48-49 -1.9597129136411482 2213 50-51 -1.4497292747372157 2213 52-53 -2.0951818677153042 2213 54-55 -1.7338726006658476 2213 56-57 -1.8069627769158956 2213 58-59 -1.7784890883733908 2213 60-61 -1.8703187917457669 2213 62-63 -1.7448031670284578 2213 64-65 -1.7242665880492751 2213 66-67 -1.4312966035755785 2213 68-69 -1.5264671318050205 2213 70-71 -1.485513868835234 2213 72-73 -1.5471377368779358 2213 74-75 -1.6821602271684881 2213 76-77 -1.5640432635712216 2213 78-79 -2.0136304660289817 2213 80-81 -1.6962161624862304 2213 82-83 -1.6901484005745147 2213 84-85 -1.3380122994343537 2213 86-87 -1.1987985430151937 2213 88-89 -1.4628708580841554 2213 90-91 -1.5123921133754301 2213 92-93 -1.6491748045841028 2213 94-95 -1.5137759954188823 2213 96-97 -1.6729750062865634 2213 98-99 -1.5601937176470848 2213 100 -1.4457274081120737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 12.0 5 12.0 6 10.0 7 20.0 8 20.0 9 33.0 10 141.0 11 579.0 12 1561.0 13 3002.0 14 4177.0 15 5124.0 16 5683.0 17 5971.0 18 6376.0 19 6986.0 20 8222.0 21 9954.0 22 11307.0 23 11886.0 24 11701.0 25 11260.0 26 11748.0 27 13088.0 28 15131.0 29 18279.0 30 22149.0 31 27676.0 32 35571.0 33 47740.0 34 65686.0 35 95127.0 36 146971.0 37 232177.0 38 175933.0 39 791.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.2105430816937 20.0125429921009 10.386891577778995 29.390022348426402 2 12.966835692184517 22.995462999257732 34.35193448037163 29.685766828186118 3 16.930570787760225 27.697483475116343 28.443153412178518 26.928792324944915 4 11.600851732366324 19.766415856845725 33.52031263123052 35.112419779557435 5 11.885536713658698 38.35539435204932 34.21508132474974 15.543987609542237 6 31.69182832604449 35.70965369437481 17.548181480280586 15.050336499300112 7 25.16644197658743 33.590936226234575 22.550416895639692 18.692204901538304 8 26.737009617380476 34.23767680461792 20.680530982198455 18.344782595803146 9 26.389572547182304 15.511498308810593 21.433527657025603 36.665401486981494 10-11 23.334761608940116 27.419135631325 27.36427798691225 21.881824772822632 12-13 25.637405247899864 25.12174823246376 27.8565985219216 21.38424799771478 14-15 24.366426263082538 25.42078864170904 25.672443897730705 24.540341197477712 16-17 21.29546488947487 28.796377881236452 28.638340119205342 21.269817110083334 18-19 22.36049767302578 27.518039115305054 30.289159531897187 19.832303679771975 20-21 22.412487084856263 26.910193612272437 29.788016228980617 20.88930307389068 22-23 21.35339074044701 26.9297570747177 28.459213426682894 23.2576387581524 24-25 21.712495614750253 27.514662792823508 27.889990760093486 22.882850832332753 26-27 22.562292455239998 26.029750573257093 28.867157025258887 22.54079994624402 28-29 21.791434602325058 25.70039291177668 30.29640331563781 22.211769170260453 30-31 22.844748781733966 26.851166481699078 28.786606040781155 21.5174786957858 32-33 20.573641235614012 26.9578676331099 29.482875142278992 22.98561598899709 34-35 20.21552676002899 27.21661412627578 30.035466616472934 22.5323924972223 36-37 22.979422835509652 26.570731131341407 28.738766200113925 21.711079833035015 38-39 23.03972886648107 26.222830450151797 28.57336241628975 22.164078267077386 40-41 21.661158849914504 26.799954349923645 28.811176166312517 22.727710633849334 42-43 22.980089294580715 26.367690716851598 28.765463144617797 21.88675684394989 44-45 23.851707074140535 25.698847062915505 27.91139340568998 22.53805245725398 46-47 22.27046812303627 26.530677788477742 29.822258214498703 21.376595873987284 48-49 23.305169126072837 26.75869818409301 28.691864070598456 21.244268619235697 50-51 22.10830957819874 27.10294089307447 28.31404510288456 22.474704425842226 52-53 21.415426915022202 27.225203072851244 26.952429708972563 24.40694030315399 54-55 22.16966261376248 27.64528342163545 26.373327719947387 23.811726244654682 56-57 22.940621048777633 26.119139344404047 27.247802385395715 23.69243722142261 58-59 22.22277669710075 25.493925947299743 28.74392829421074 23.53936906138877 60-61 22.867598886553566 26.62478890664263 27.713828060075514 22.79378414672829 62-63 20.855045037477705 26.960229659019603 28.124237228675753 24.060488074826942 64-65 20.412725157063342 27.099485390576938 28.661249638686375 23.826539813673342 66-67 22.779019643884187 26.4874723050682 27.66488984285288 23.068618208194735 68-69 23.123897133126746 26.03766461778235 27.322586367663636 23.515851881427267 70-71 21.723223431711617 26.669018771874946 27.589304641602908 24.018453154810523 72-73 22.949031908669735 26.308395790838514 27.727179292569932 23.015393007921826 74-75 23.664628713935375 25.69703072938877 26.976627203877136 23.66171335279872 76-77 22.102803045650166 26.479714606175296 28.87680532840548 22.540677019769056 78-79 22.929780527090724 26.593377274029862 28.250491615364094 22.226350583515323 80-81 21.717534791959718 26.734731286552744 28.149680897686167 23.39805302380137 82-83 21.026600001976227 26.813384978706168 27.143612343507606 25.016402675810006 84-85 21.66194831053206 26.9927520120948 26.513011299351284 24.832288378021847 86-87 22.473324562153262 25.978963989579324 27.10783759120932 24.439873857058092 88-89 21.853264838084623 25.35752010047024 28.419846626767097 24.36936843467804 90-91 22.238586301635163 26.181066864418963 27.670532948039135 23.90981388590674 92-93 20.54332903029468 26.649000000494034 27.84772479918741 24.959946170023876 94-95 20.08227516978455 26.732612614570918 28.310137966774747 24.87497424886979 96-97 22.11561215078162 26.162002846558067 27.482129968446777 24.240255034213533 98-99 22.120332911603146 25.75129643745853 27.35168098425008 24.776689666688238 100 20.99179864141508 26.23910540704138 28.239497078290732 24.52959887325281 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 32.0 1 24.5 2 20.0 3 19.0 4 23.5 5 74.5 6 255.5 7 578.5 8 959.0 9 1085.0 10 765.5 11 444.5 12 391.5 13 431.0 14 484.0 15 551.5 16 604.5 17 666.5 18 777.0 19 884.0 20 1030.0 21 1241.0 22 1526.0 23 1966.5 24 2593.5 25 3464.5 26 4713.5 27 6271.5 28 8080.5 29 10278.5 30 12667.0 31 14960.5 32 17180.5 33 19433.5 34 21578.0 35 23337.5 36 25245.5 37 27435.0 38 28906.0 39 30029.0 40 30944.5 41 31023.0 42 30947.5 43 30862.0 44 30359.0 45 29996.5 46 30679.5 47 32758.5 48 34431.0 49 41351.5 50 70179.5 51 70932.0 52 39450.5 53 27382.0 54 24722.5 55 23136.5 56 21614.5 57 19881.0 58 18082.0 59 16674.5 60 15105.0 61 13422.5 62 11605.5 63 9412.0 64 7452.5 65 6168.0 66 5109.5 67 4182.0 68 3342.0 69 2611.0 70 1913.0 71 1411.5 72 1136.5 73 896.0 74 699.5 75 536.0 76 311.5 77 167.5 78 115.0 79 55.0 80 25.5 81 12.5 82 7.5 83 3.0 84 1.5 85 2.0 86 1.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7474483998774837 2 5.225321358350377 3 0.0 4 0.005434191935659168 5 0.1635197755184713 6 0.05118020768493543 7 0.034186007449783125 8 0.03952139589570304 9 0.039126181936746 10-11 0.03957079764057266 12-13 0.038632164488049715 14-15 0.04036122555848672 16-17 0.03225933939986761 18-19 0.027763780616731387 20-21 0.02139095552854927 22-23 0.021193348549070755 24-25 0.019266680499155232 26-27 0.012795051921233858 28-29 0.01758702117358785 30-31 0.021835571232375926 32-33 0.0013832488563496064 34-35 0.003112309926786614 36-37 0.004100344824179191 38-39 0.0061752181087036 40-41 0.006274021598442857 42-43 0.00657043206766063 44-45 0.008497100117576152 46-47 0.016253174062107873 48-49 0.024355060220726997 50-51 0.022082579956724073 52-53 0.027763780616731387 54-55 0.024009248006639596 56-57 0.015857960103150843 58-59 0.013980693798104949 60-61 0.011560008299493139 62-63 0.01729061070437008 64-65 0.01753761942871822 66-67 0.018970270029937456 68-69 0.025540702097598084 70-71 0.024750274179684024 72-73 0.021934374722115187 74-75 0.02257659740542036 76-77 0.01729061070437008 78-79 0.013832488563496063 80-81 0.012893855410973116 82-83 0.007904279179140607 84-85 0.010374366422622046 86-87 0.008250091393228009 88-89 0.006817440792008774 90-91 0.006866842536878402 92-93 0.003408720396004387 94-95 0.0018278645601762652 96-97 0.0010374366422622047 98-99 9.386331525229471E-4 100 0.10453409214413453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1012110.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.2407601024128 #Duplication Level Percentage of deduplicated Percentage of total 1 81.98035873199669 53.4846091714394 2 11.681741491592721 15.242513884628057 3 3.4176790272867588 6.68915932578789 4 1.313155582115879 3.4268507323986523 5 0.582791436753582 1.9010878157490467 6 0.31786516328499803 1.24426589176745 7 0.18087653530822573 0.8260365853739688 8 0.1067574357842251 0.5571949005717897 9 0.07929152978010452 0.4655735705283408 >10 0.30611261074861085 3.248841994394015 >50 0.014715835162347759 0.6992222564222141 >100 0.012284750582279211 1.6074843437458801 >500 0.0018199627438890615 0.8077989046744523 >1k 0.003943252611759633 5.7444357189833415 >5k 3.0332712398151027E-4 1.0395315154960554 >10k+ 3.0332712398151027E-4 3.015393388039433 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 17922 1.7707561431069747 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12553 1.2402802066969005 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5291 0.5227692642104119 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 5215 0.5152601989902283 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4774 0.4716878600152157 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4525 0.44708579107014057 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 4272 0.4220885081661084 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3698 0.36537530505577454 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3521 0.347887087371926 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2848 0.2813923387774056 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2756 0.27230241772139396 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 2644 0.26123642687059706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2349 0.23208939739751605 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1951 0.19276560848129157 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1882 0.1859481676892828 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 1808 0.1786367094485777 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1773 0.17517858730770372 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1751 0.17300491053344005 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1741 0.17201687563604748 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 1694 0.16737311161830237 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 1655 0.1635197755184713 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 1596 0.1576903696238551 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 1561 0.1542322474829811 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1515 0.14968728695497524 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 1501 0.14830403809862563 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 1351 0.133483514637737 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1308 0.12923496457894892 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1232 0.12172589935876535 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 1222 0.12073786446137277 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 1128 0.11145033642588256 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.976069794785152E-4 0.0 5 0.0 0.0 0.0 2.9641046921777275E-4 0.0 6 0.0 0.0 0.0 2.9641046921777275E-4 0.0 7 0.0 0.0 0.0 3.952139589570304E-4 0.0 8 0.0 0.0 0.0 3.952139589570304E-4 0.0 9 0.0 0.0 0.0 3.952139589570304E-4 0.0 10-11 0.0 0.0 0.0 3.952139589570304E-4 0.0 12-13 0.0 0.0 0.0 3.952139589570304E-4 0.0 14-15 0.0 0.0 0.0 3.952139589570304E-4 0.0 16-17 0.0 0.0 0.0 4.4461570382665916E-4 0.0 18-19 0.0 0.0 0.0 5.434191935659167E-4 0.0 20-21 0.0 0.0 0.0 7.41026173044432E-4 0.0 22-23 0.0 0.0 0.0 9.386331525229471E-4 0.0 24-25 0.0 0.0 0.0 0.0018278645601762654 0.0 26-27 4.94017448696288E-5 0.0 0.0 0.002914702947308099 0.0 28-29 9.88034897392576E-5 0.0 0.0 0.005187183211311024 0.0 30-31 9.88034897392576E-5 0.0 0.0 0.01353607809427829 0.0 32-33 9.88034897392576E-5 0.0 0.0 0.03640908596891643 0.0 34-35 9.88034897392576E-5 0.0 0.0 0.07010107597000326 0.0 36-37 9.88034897392576E-5 0.0 0.0 0.11965102607424094 0.0 38-39 9.88034897392576E-5 0.0 0.0 0.19355603639920563 0.0 40-41 9.88034897392576E-5 0.0 0.0 0.30614261295708967 0.0 42-43 9.88034897392576E-5 0.0 0.0 0.39437412929424664 0.0 44-45 9.88034897392576E-5 0.0 0.0 0.49579591151159463 0.0 46-47 9.88034897392576E-5 0.0 0.0 0.6221161731432354 0.0 48-49 9.88034897392576E-5 0.0 0.0 0.7398899329124304 0.0 50-51 9.88034897392576E-5 0.0 0.0 0.8693719062157275 0.0 52-53 9.88034897392576E-5 0.0 0.0 1.0230607345051428 0.0 54-55 9.88034897392576E-5 0.0 0.0 1.1778858029265593 0.0 56-57 9.88034897392576E-5 0.0 0.0 1.3483218227267786 0.0 58-59 9.88034897392576E-5 0.0 0.0 1.545928802205294 0.0 60-61 9.88034897392576E-5 0.0 0.0 1.7485747596605112 0.0 62-63 9.88034897392576E-5 0.0 0.0 1.9542342235527759 0.0 64-65 9.88034897392576E-5 0.0 0.0 2.1963521751588266 0.0 66-67 9.88034897392576E-5 0.0 0.0 2.4608491171908193 0.0 68-69 9.88034897392576E-5 0.0 0.0 2.7668435249133 0.0 70-71 9.88034897392576E-5 0.0 0.0 3.18073134343105 0.0 72-73 9.88034897392576E-5 0.0 0.0 3.553516910217269 0.0 74-75 9.88034897392576E-5 0.0 0.0 3.927438717135489 0.0 76-77 9.88034897392576E-5 0.0 0.0 4.348983806108032 0.0 78-79 9.88034897392576E-5 0.0 0.0 4.795130964025649 0.0 80-81 9.88034897392576E-5 0.0 0.0 5.275661736372529 0.0 82-83 9.88034897392576E-5 0.0 0.0 5.861862840995544 0.0 84-85 9.88034897392576E-5 0.0 0.0 6.439270435031766 0.0 86-87 9.88034897392576E-5 0.0 0.0 7.017468456985901 0.0 88 9.88034897392576E-5 0.0 0.0 7.514993429567932 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 470 0.0 21.016077 5 CCTATAC 210 9.455798E-7 20.158278 3 CCTAGAC 280 1.7789716E-9 20.158276 3 GAGTACT 3760 0.0 19.041258 12-13 GCCTAAG 190 1.3265837E-4 18.296803 1 AGTACTT 3925 0.0 18.180994 12-13 CTACGAC 130 0.008002884 18.090761 3 GCACCGT 130 0.008069371 18.064817 6 GTACTTT 4080 0.0 17.662895 14-15 CATGGGG 2580 0.0 16.95483 4 TACTAAG 265 5.2269625E-6 16.85602 2 GTCTAGC 180 0.0019447985 16.554253 1 GTCTATA 210 2.8509612E-4 16.55425 1 AAAGTAC 540 0.0 16.549696 3 CATGGGT 1285 0.0 16.471745 4 CTAGTCT 200 2.974458E-4 16.462593 4 GTACTAA 280 8.768195E-6 15.963027 1 TAGACAG 415 8.3491614E-10 15.867559 5 GTGTATA 285 1.0371061E-5 15.682975 1 GTTTAGG 255 7.051796E-5 15.580472 1 >>END_MODULE