FastQCFastQC Report
Wed 25 May 2016
SRR1295117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535059
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5680.10615651731865083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3500.050.563261
GTATCAA6400.047.6754951
CCGTGAT650.001714299137.538252
TCAACGC7200.037.521964
TATCAAC7450.036.845432
ATCAACG7350.036.7516753
CAACGCA7250.035.8459975
AACGCAG7350.033.3183986
GACCGTG1003.5613426E-529.9063537
GAACCGC700.00654318928.5586266
ACGCAGA8700.026.3542587
TGGTATC2409.942596E-925.4165252
GTATATA1701.1469181E-525.1277891
GGACCGT1000.001228589824.9887966
ATAATAC1801.4144043E-624.5011163
GTCCTAT1250.001407924824.4098511
CGCAGAG9550.024.0783588
GTATAAA2754.1998646E-822.1907731
GTGGTAT2203.3440592E-622.1907731
GTACATG13400.021.404161
Produced by FastQC (version 0.11.5)