##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295117_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 535059 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.01267710663684 27.0 27.0 33.0 6.0 33.0 2 22.080529436940598 27.0 2.0 33.0 2.0 33.0 3 24.32324846418806 27.0 15.0 33.0 6.0 33.0 4 24.722049344091026 33.0 6.0 37.0 2.0 37.0 5 26.60422495463117 33.0 15.0 37.0 2.0 37.0 6 27.280886780710166 33.0 15.0 37.0 2.0 37.0 7 26.911297632597527 33.0 15.0 37.0 6.0 37.0 8 27.659211414068356 33.0 15.0 37.0 6.0 37.0 9 28.584210339420512 33.0 22.0 37.0 6.0 37.0 10-11 28.17786356270991 33.0 18.5 37.0 6.0 37.0 12-13 27.025382247565222 33.0 15.0 37.0 4.0 37.0 14-15 28.692042372897195 33.0 18.5 37.0 4.0 40.0 16-17 29.11300155683766 33.0 22.0 37.0 6.0 40.0 18-19 29.230508224326663 33.0 22.0 38.5 6.0 40.0 20-21 28.671846469267877 33.0 18.5 37.0 6.0 40.0 22-23 29.44690118286021 33.0 22.0 37.0 6.0 40.0 24-25 29.56531148901336 33.0 24.5 37.0 6.0 40.0 26-27 28.125199277089067 33.0 18.5 37.0 4.0 40.0 28-29 28.867171657705036 33.0 22.0 37.0 6.0 40.0 30-31 29.57717747014815 33.0 24.5 37.0 6.0 40.0 32-33 28.284720002840807 33.0 22.0 37.0 4.0 40.0 34-35 27.962028486578113 33.0 18.5 37.0 6.0 40.0 36-37 28.193941228911207 33.0 22.0 37.0 6.0 40.0 38-39 28.3385280875567 33.0 22.0 37.0 6.0 40.0 40-41 27.42172452009965 33.0 15.0 37.0 6.0 40.0 42-43 28.053382897960788 33.0 22.0 37.0 6.0 40.0 44-45 27.8529405168402 33.0 22.0 37.0 6.0 40.0 46-47 27.495614502325914 33.0 18.5 37.0 6.0 40.0 48-49 27.38426229630751 33.0 18.5 37.0 6.0 40.0 50-51 27.20977593125244 33.0 18.5 37.0 6.0 40.0 52-53 27.440378537693974 33.0 18.5 37.0 6.0 40.0 54-55 27.37357842779955 33.0 22.0 37.0 6.0 40.0 56-57 27.352375158627368 33.0 22.0 37.0 6.0 40.0 58-59 27.23240708034067 33.0 22.0 37.0 6.0 40.0 60-61 27.190024838382307 33.0 22.0 37.0 6.0 40.0 62-63 27.106411629371713 33.0 22.0 37.0 6.0 38.5 64-65 26.59534088764043 30.0 18.5 37.0 6.0 37.0 66-67 26.799171680132474 27.0 22.0 37.0 6.0 37.0 68-69 26.364790611876447 30.0 18.5 37.0 6.0 37.0 70-71 26.53508304691632 27.0 22.0 37.0 6.0 37.0 72-73 26.577492388689848 27.0 22.0 33.0 6.0 37.0 74-75 26.69310767597592 27.0 22.0 33.0 6.0 37.0 76-77 26.48792749958416 27.0 22.0 33.0 6.0 37.0 78-79 26.038782638923934 27.0 22.0 33.0 6.0 37.0 80-81 25.861381642024526 27.0 22.0 33.0 6.0 37.0 82-83 25.992463447956204 27.0 22.0 33.0 6.0 37.0 84-85 25.926309995720096 27.0 22.0 33.0 6.0 37.0 86-87 25.738034497130222 27.0 22.0 33.0 6.0 37.0 88-89 25.68990615988143 27.0 22.0 33.0 6.0 37.0 90-91 25.847026215800497 27.0 22.0 33.0 6.0 37.0 92-93 25.776501283036076 27.0 22.0 33.0 6.0 37.0 94-95 25.629847362627302 27.0 22.0 33.0 6.0 37.0 96-97 25.689129610005626 27.0 22.0 33.0 6.0 37.0 98-99 25.75476816575368 27.0 22.0 33.0 6.0 37.0 100 25.44936165918151 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.240245190002824 1101 2 11.174312383370054 1101 3 8.260456833558852 1101 4 12.350634324338245 1101 5 10.544119271636447 1101 6 9.559611818533842 1101 7 9.236488519097069 1101 8 8.754607972991973 1101 9 7.084613484910832 1101 10-11 7.72460183095702 1101 12-13 8.85170450729482 1101 14-15 7.688873900240512 1101 16-17 7.902154082019528 1101 18-19 7.624782662919639 1101 20-21 7.001673286296839 1101 22-23 6.9515179207774835 1101 24-25 6.967996423672748 1101 26-27 8.257093071720195 1101 28-29 7.787920362301701 1101 30-31 5.06607178600412 1101 32-33 6.894604491283857 1101 34-35 8.628373251181884 1101 36-37 8.122020623815565 1101 38-39 7.983761832426914 1101 40-41 8.980992779546487 1101 42-43 8.390041740349737 1101 44-45 8.415176184868436 1101 46-47 7.226043835216036 1101 48-49 5.001683138661129 1101 50-51 6.524753917575289 1101 52-53 5.536767556035105 1101 54-55 3.7214970368773344 1101 56-57 5.8696853674973966 1101 58-59 5.991215272237291 1101 60-61 6.704382640510733 1101 62-63 6.11887482682647 1101 64-65 6.4116653147801514 1101 66-67 5.490815506931561 1101 68-69 6.315178268522043 1101 70-71 6.22065508753569 1101 72-73 5.2615193853811135 1101 74-75 4.557435289459335 1101 76-77 4.762052604321315 1101 78-79 4.549468456117495 1101 80-81 4.715799385806147 1101 82-83 4.320266749771985 1101 84-85 4.011542491275968 1101 86-87 3.5840590521496374 1101 88-89 3.726894351376476 1101 90-91 3.6458705413893107 1101 92-93 3.3203105906111645 1101 94-95 3.5066683637329454 1101 96-97 3.224789232144321 1101 98-99 3.037763906522862 1101 100 3.151771314917074 1102 1 6.798646279692679 1102 2 10.356966057580728 1102 3 8.085720873793555 1102 4 12.315526752390774 1102 5 10.460843216872547 1102 6 9.448257389922492 1102 7 6.6799042776888555 1102 8 7.531039238708654 1102 9 6.437788254399514 1102 10-11 7.333223303435368 1102 12-13 8.829489503103709 1102 14-15 6.157420980430512 1102 16-17 7.279690405014783 1102 18-19 7.3508493016455425 1102 20-21 7.21203896106633 1102 22-23 6.483389952071143 1102 24-25 7.010034702521597 1102 26-27 8.67571908457295 1102 28-29 7.605038621592005 1102 30-31 4.020174538169531 1102 32-33 4.806845340683125 1102 34-35 5.167292991332761 1102 36-37 5.1679748009600175 1102 38-39 4.481946381183548 1102 40-41 6.206987121541459 1102 42-43 6.839362779746217 1102 44-45 6.534741705963718 1102 46-47 5.749654829907289 1102 48-49 3.7224937701224263 1102 50-51 5.2812606233590245 1102 52-53 5.9969390424838345 1102 54-55 5.55835963816261 1102 56-57 6.243165739109578 1102 58-59 5.920703606967663 1102 60-61 5.734015219443389 1102 62-63 4.095376293716665 1102 64-65 5.8936836858335155 1102 66-67 3.650006272508538 1102 68-69 6.027316784867391 1102 70-71 5.280591871553575 1102 72-73 4.869077362469685 1102 74-75 4.071918385296158 1102 76-77 3.281554560174907 1102 78-79 4.236264348824953 1102 80-81 4.339183711036657 1102 82-83 3.9326308877993696 1102 84-85 3.048215793874455 1102 86-87 3.112856202191548 1102 88-89 3.303165306950657 1102 90-91 3.241868725239552 1102 92-93 3.4658037451235977 1102 94-95 3.281557648449347 1102 96-97 3.5133694919934406 1102 98-99 3.3931683491045916 1102 100 3.263436584545463 1103 1 6.571453079029329 1103 2 10.966588777315387 1103 3 7.70373082404231 1103 4 11.865360914911506 1103 5 10.344035588348504 1103 6 8.859762365046873 1103 7 8.23190261931406 1103 8 7.972486170715289 1103 9 6.997137342293378 1103 10-11 7.5361980132197814 1103 12-13 8.761375904430409 1103 14-15 8.356409371806958 1103 16-17 7.959138995396209 1103 18-19 7.212752286720171 1103 20-21 8.46746391960696 1103 22-23 6.898414827693024 1103 24-25 5.0955487987073305 1103 26-27 5.815084756214741 1103 28-29 6.873983480630148 1103 30-31 5.96656964596389 1103 32-33 7.871737546321604 1103 34-35 7.257684782377538 1103 36-37 4.423712777742772 1103 38-39 6.449371754317873 1103 40-41 6.650562992188227 1103 42-43 7.449080856031458 1103 44-45 5.4008694008227565 1103 46-47 6.2302290421792605 1103 48-49 5.793752061996393 1103 50-51 6.555079031319224 1103 52-53 6.063914581455638 1103 54-55 4.630944629870736 1103 56-57 5.471414080734789 1103 58-59 5.078400538974293 1103 60-61 4.749960493075232 1103 62-63 2.47020423733192 1103 64-65 4.638801845037499 1103 66-67 3.7923881133045576 1103 68-69 5.027492987189117 1103 70-71 3.4210831650859106 1103 72-73 4.380851823497878 1103 74-75 2.8557575229412677 1103 76-77 3.172586898483363 1103 78-79 2.9826615295878085 1103 80-81 3.966012069245611 1103 82-83 3.342048383653431 1103 84-85 3.3990221786174075 1103 86-87 2.7758661524403045 1103 88-89 2.914684792854473 1103 90-91 2.5338795046757028 1103 92-93 2.2003974432993836 1103 94-95 2.486800185762778 1103 96-97 2.7036866561725432 1103 98-99 2.438238830033278 1103 100 3.036985506601848 1104 1 6.307211571788901 1104 2 9.847318291257704 1104 3 7.379604035305583 1104 4 12.180131563387334 1104 5 9.443850089724783 1104 6 8.104056358994661 1104 7 4.165935205523908 1104 8 6.602078757609 1104 9 6.7020064674579345 1104 10-11 7.193854746734338 1104 12-13 7.607298781454222 1104 14-15 7.313881461530162 1104 16-17 7.2423278620594544 1104 18-19 6.847644834291589 1104 20-21 7.442919545258771 1104 22-23 6.314901979562542 1104 24-25 6.076374908707155 1104 26-27 8.085126301067792 1104 28-29 7.111838449770698 1104 30-31 6.534250998650624 1104 32-33 7.714010289136738 1104 34-35 8.085613438068151 1104 36-37 6.454469646320845 1104 38-39 7.173277996303817 1104 40-41 7.242884887864484 1104 42-43 7.357043192117352 1104 44-45 6.584312152699116 1104 46-47 6.8704151391856385 1104 48-49 5.697901845723802 1104 50-51 5.448915262534285 1104 52-53 5.797080795061152 1104 54-55 4.38419726702859 1104 56-57 5.923058350793429 1104 58-59 3.800776631022643 1104 60-61 4.332056456556796 1104 62-63 3.6484218263627106 1104 64-65 2.433911349063756 1104 66-67 3.1209899494844855 1104 68-69 3.9218399807436803 1104 70-71 2.601386545093092 1104 72-73 2.5497474655624686 1104 74-75 3.2571761172549216 1104 76-77 3.583521914126795 1104 78-79 4.285723111711555 1104 80-81 3.029278212754871 1104 82-83 2.6400664548096806 1104 84-85 2.7338386461081328 1104 86-87 0.5453819753874249 1104 88-89 0.2971902210400046 1104 90-91 2.228990718366699 1104 92-93 2.430133642030814 1104 94-95 2.2470774078995213 1104 96-97 2.52699921707935 1104 98-99 2.494860788967131 1104 100 0.7837114986348084 1105 1 5.424650799002457 1105 2 -2.84688425740147 1105 3 0.4380133963901045 1105 4 8.071716837299018 1105 5 8.286536177705198 1105 6 7.88124492758341 1105 7 4.887665165116957 1105 8 5.705449674205781 1105 9 4.509244704687109 1105 10-11 4.681674070348542 1105 12-13 4.595654252487435 1105 14-15 5.547267597795614 1105 16-17 5.666509769572436 1105 18-19 5.905592522680873 1105 20-21 6.919272938058935 1105 22-23 1.486723285404473 1105 24-25 3.4519971785555548 1105 26-27 6.64562801363348 1105 28-29 5.834346687875215 1105 30-31 5.08111571866802 1105 32-33 6.545330687163542 1105 34-35 5.834194182787158 1105 36-37 5.83424979411258 1105 38-39 3.8817211004385435 1105 40-41 5.079377220144583 1105 42-43 5.2635534124495855 1105 44-45 5.81128922719078 1105 46-47 4.8027543231967975 1105 48-49 4.597040127284849 1105 50-51 5.436878947105946 1105 52-53 5.22997553933401 1105 54-55 4.7919446148813165 1105 56-57 4.758784405228454 1105 58-59 1.7332347628153748 1105 60-61 4.0068733405061145 1105 62-63 -1.321054478674462 1105 64-65 -0.7670376918712201 1105 66-67 0.5550476995148372 1105 68-69 2.619978042057202 1105 70-71 2.641911030688057 1105 72-73 2.1759658861618227 1105 74-75 2.189799787651676 1105 76-77 1.1813689945764416 1105 78-79 2.9801201417583982 1105 80-81 1.2817658803053504 1105 82-83 0.6487192882376043 1105 84-85 0.6193240518859611 1105 86-87 -0.703491373815023 1105 88-89 1.1466153891199262 1105 90-91 0.8841318038481525 1105 92-93 1.100238146657425 1105 94-95 0.4961402970387745 1105 96-97 1.7132986209991898 1105 98-99 1.6535812496797746 1105 100 1.7658057984594606 1106 1 5.699423871665925 1106 2 10.06114387251941 1106 3 6.704550305231681 1106 4 11.287879037786535 1106 5 9.567794164475671 1106 6 9.305684926154381 1106 7 6.2851383914012295 1106 8 7.318171345732068 1106 9 6.47119126443296 1106 10-11 5.328882445018422 1106 12-13 7.331521275678959 1106 14-15 7.197147847766065 1106 16-17 7.381326413933401 1106 18-19 6.848784084254238 1106 20-21 7.765387623273686 1106 22-23 5.9578771315583126 1106 24-25 4.805530800626386 1106 26-27 7.159930344327687 1106 28-29 5.772347707433699 1106 30-31 4.93148864073363 1106 32-33 7.262804092076664 1106 34-35 6.665892489660521 1106 36-37 5.538699438641181 1106 38-39 6.943922412398713 1106 40-41 6.390820733581595 1106 42-43 5.794730672722807 1106 44-45 3.7560070348377437 1106 46-47 5.397329021657566 1106 48-49 5.160655698773489 1106 50-51 6.1147472454326035 1106 52-53 5.160527392539574 1106 54-55 4.752876081863835 1106 56-57 4.253050075787396 1106 58-59 4.611097977090292 1106 60-61 4.396233725574941 1106 62-63 4.600229972646428 1106 64-65 4.762628780593829 1106 66-67 2.578260174403745 1106 68-69 2.7200634403857826 1106 70-71 2.3137956062247014 1106 72-73 2.3260450324808346 1106 74-75 2.248863758763271 1106 76-77 2.89006347879252 1106 78-79 3.573413178256395 1106 80-81 3.199576687625285 1106 82-83 1.5265508989476437 1106 84-85 2.166347064777465 1106 86-87 3.001602021589541 1106 88-89 2.974156109626243 1106 90-91 2.5943408545595084 1106 92-93 2.6153745366509114 1106 94-95 0.8357535793300634 1106 96-97 1.114945578949964 1106 98-99 1.7041466099741562 1106 100 2.5115413783024323 1107 1 6.0148843127649165 1107 2 10.230993472724329 1107 3 5.843579881626454 1107 4 11.186849641859876 1107 5 9.157444609387781 1107 6 7.855709522211441 1107 7 3.64375545262358 1107 8 5.962916958464966 1107 9 5.390018454573582 1107 10-11 4.366126915271746 1107 12-13 6.135174881693786 1107 14-15 6.012660318985773 1107 16-17 6.779448890654471 1107 18-19 6.086821886501934 1107 20-21 5.0213416155484865 1107 22-23 5.2439034420624395 1107 24-25 4.261901543252662 1107 26-27 7.21345842312822 1107 28-29 6.529143277814807 1107 30-31 5.0745729280737955 1107 32-33 4.678725236244485 1107 34-35 6.612587812916747 1107 36-37 6.7096893353377425 1107 38-39 6.172479453420273 1107 40-41 7.485841560183758 1107 42-43 6.022374093932463 1107 44-45 4.0271916624476205 1107 46-47 3.4947668838672534 1107 48-49 3.9244356761276507 1107 50-51 4.524809361392098 1107 52-53 3.561502793571737 1107 54-55 5.024139664272269 1107 56-57 4.9877055563419574 1107 58-59 2.534236026462878 1107 60-61 2.692931668529557 1107 62-63 0.3063325600352016 1107 64-65 1.5849750261294275 1107 66-67 3.682376812108192 1107 68-69 4.869957124292977 1107 70-71 4.106809234477858 1107 72-73 2.051888502591531 1107 74-75 2.38663988225003 1107 76-77 2.670830017093966 1107 78-79 3.5392952452566355 1107 80-81 1.8471319268765178 1107 82-83 1.484033194152719 1107 84-85 2.1677838965724554 1107 86-87 2.461842277039242 1107 88-89 2.6234992930255068 1107 90-91 1.3577064101829812 1107 92-93 2.2153076615726803 1107 94-95 2.3068481184232397 1107 96-97 2.1204702317671575 1107 98-99 1.83941073378692 1107 100 0.31639567941056157 1108 1 4.689068194050364 1108 2 -2.716339987759323 1108 3 0.39640097454921985 1108 4 6.935048162970119 1108 5 7.1966156769900955 1108 6 7.193922797143319 1108 7 4.392276234028067 1108 8 5.3479347047036185 1108 9 5.095334434080456 1108 10-11 3.6987302303875076 1108 12-13 2.6860952965545906 1108 14-15 4.187672869599272 1108 16-17 4.944784863453069 1108 18-19 4.245742248748815 1108 20-21 5.048467382560869 1108 22-23 4.447260648964509 1108 24-25 3.134136297819662 1108 26-27 4.736497841919714 1108 28-29 6.068286942330882 1108 30-31 4.435347711973055 1108 32-33 4.37704685201814 1108 34-35 3.132222672273148 1108 36-37 1.0658505356367627 1108 38-39 2.875252426523595 1108 40-41 5.623083888963794 1108 42-43 2.9565080358335365 1108 44-45 3.7456359125128387 1108 46-47 4.981931278270007 1108 48-49 5.177232435109836 1108 50-51 2.505368515886314 1108 52-53 0.655705835095084 1108 54-55 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14-15 3.681459909870899 1109 16-17 4.026472643457602 1109 18-19 4.000283608214886 1109 20-21 4.827394800482388 1109 22-23 5.486723285404473 1109 24-25 5.482431296195561 1109 26-27 7.025202052942777 1109 28-29 6.778527064414387 1109 30-31 6.133060791210898 1109 32-33 7.857477944089453 1109 34-35 2.523999195712321 1109 36-37 5.246490616045179 1109 38-39 4.236149544200579 1109 40-41 6.661357559497663 1109 42-43 2.942574458578335 1109 44-45 4.978598153652285 1109 46-47 5.060399963727239 1109 48-49 4.367186592506858 1109 50-51 6.347957703650998 1109 52-53 4.652453032751474 1109 54-55 5.781154650328062 1109 56-57 6.455632284121744 1109 58-59 4.396984020592967 1109 60-61 2.684149039394729 1109 62-63 3.7070824980962236 1109 64-65 5.305377724762955 1109 66-67 4.4743523795645 1109 68-69 3.4927821133345454 1109 70-71 1.6318722851788792 1109 72-73 2.6718754159976648 1109 74-75 3.4599597478265736 1109 76-77 2.2452346088367072 1109 78-79 2.8886183634234968 1109 80-81 2.0196399154162172 1109 82-83 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2212 94-95 -3.4027875896458895 2212 96-97 -5.499547174673225 2212 98-99 -3.6440935556573137 2212 100 -3.396087224978345 2213 1 -21.03385372030732 2213 2 -16.94220060908594 2213 3 -19.694279126206446 2213 4 -18.66780658094256 2213 5 -21.017490116460785 2213 6 -21.672575943410838 2213 7 -10.567704417963318 2213 8 -10.438852065639171 2213 9 -4.297537832557005 2213 10-11 -17.532990464680577 2213 12-13 -16.098662670809336 2213 14-15 -23.345912352902825 2213 16-17 -17.83991104426058 2213 18-19 -18.282737654876197 2213 20-21 -17.493558865020628 2213 22-23 -19.678494105899876 2213 24-25 -7.64547254385522 2213 26-27 -22.978447582093718 2213 28-29 -23.92037804507466 2213 30-31 -19.154046476323224 2213 32-33 -23.149821325983538 2213 34-35 -17.7628700521455 2213 36-37 -23.428691865706647 2213 38-39 -11.277147821715005 2213 40-41 -16.826654182806365 2213 42-43 -22.940637220253784 2213 44-45 -16.822504670847874 2213 46-47 -16.750580677339087 2213 48-49 -6.411835940022499 2213 50-51 -22.931239376640974 2213 52-53 -17.53336892853066 2213 54-55 -17.29553528937362 2213 56-57 -17.10681614494839 2213 58-59 -17.56590871187292 2213 60-61 -18.77040507041168 2213 62-63 -17.185221532370292 2213 64-65 -17.206769212717205 2213 66-67 -8.443562568071172 2213 68-69 -22.10393321513261 2213 70-71 -16.958194360330484 2213 72-73 -9.19795162303756 2213 74-75 -4.595617846587899 2213 76-77 -3.2843512369265433 2213 78-79 -5.12667043378374 2213 80-81 -3.418316288963343 2213 82-83 -3.376626358577443 2213 84-85 -2.893686380038588 2213 86-87 -3.328665536938889 2213 88-89 -2.696997736527603 2213 90-91 -2.3962109849053768 2213 92-93 -3.6830368345865487 2213 94-95 -3.9165039457535507 2213 96-97 -3.5991848558326467 2213 98-99 -3.9523751291562803 2213 100 -3.8785924009617823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 149.0 3 169.0 4 869.0 5 2851.0 6 4051.0 7 5261.0 8 5151.0 9 5298.0 10 6245.0 11 7470.0 12 9203.0 13 11406.0 14 9408.0 15 10410.0 16 11749.0 17 12221.0 18 12009.0 19 12141.0 20 11682.0 21 11672.0 22 12268.0 23 13202.0 24 14109.0 25 15359.0 26 16343.0 27 17506.0 28 18560.0 29 19463.0 30 20862.0 31 23162.0 32 26456.0 33 31236.0 34 36547.0 35 40375.0 36 41376.0 37 31094.0 38 7720.0 39 6.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.01660579776307 20.138096325039946 13.812078129585547 25.03321974761144 2 16.53310372087988 21.431754495947793 37.74405764481069 24.291084138361644 3 18.640721431477843 25.88249678791414 29.138679927920997 26.33810185268703 4 14.449422042553605 18.420784117466997 34.99497111427475 32.13482272570465 5 14.750859955080497 36.79963583425305 33.87084999205535 14.578654218611103 6 31.39447065345872 36.75938032824611 17.358758982834548 14.487390035460624 7 26.970406214551357 32.20731854909016 21.62395564316791 19.198319593190575 8 25.44983327280531 34.54513189606788 20.67891808907524 19.326116742051568 9 25.31206795175362 17.71715638756019 20.9546379137731 36.01613774691309 10-11 24.34714101834703 27.221556451320044 27.396270875952762 21.03503165438017 12-13 24.491490158110125 25.616516558590245 27.0604398476823 22.831553435617334 14-15 22.446131988450936 27.10837063185268 24.8300845229705 25.61541285672589 16-17 22.11792329447923 29.57308849253707 25.677835177949614 22.631153035034085 18-19 22.262301221363597 28.803724091829793 27.119365841283138 21.814608845523477 20-21 23.682833547515063 27.87284260970627 26.405074827075985 22.039249015702676 22-23 23.507927970997173 27.608760607147126 26.420062945699375 22.463248476156327 24-25 23.387524139751548 27.766519727610316 26.14109325039646 22.704862882241677 26-27 23.188694599782988 27.456597946749017 26.530965695684834 22.823741757783157 28-29 22.968650609710824 27.46899273805009 26.624507626151043 22.93784902608804 30-31 23.227027614549804 27.617487419668503 26.397790130988202 22.75769483479349 32-33 23.058565598052837 27.49655757570753 26.3808405089214 23.06403631731823 34-35 23.135806971161152 27.159672207534364 26.22106745272029 23.483453368584193 36-37 23.342137711003648 27.472495184947654 26.124685793138664 23.060681310910034 38-39 23.106099200664882 27.29536641354055 26.296946703666634 23.301587682127938 40-41 23.296074448108524 27.208103730703392 26.16819461902611 23.32762720216198 42-43 23.172622674864098 27.364152131110615 26.379997948631317 23.08322724539397 44-45 23.3115516557256 27.187149982169494 26.290903428981853 23.21039493312305 46-47 23.20667861748466 27.294577016900163 26.31432381393455 23.184420551680635 48-49 23.177423339723113 27.191950243693796 26.275376429033887 23.355249987549204 50-51 23.191640213345366 26.97936640938462 26.344688119370275 23.48430525789974 52-53 23.0205620496917 27.027401964015674 26.268439088654223 23.683596897638402 54-55 23.317619433230444 27.133503473755276 26.218388232988104 23.330488860026176 56-57 23.188440141950696 27.1587347666641 26.10861953540177 23.544205555983435 58-59 23.0375758518749 26.78290804418858 26.305721954255485 23.873794149681032 60-61 23.19463355282413 27.032214931965033 26.15017320750543 23.62297830770541 62-63 23.121734918137875 26.90586991981544 26.288894539850073 23.68350062219661 64-65 23.179154684507537 26.81149578680713 26.151714309896523 23.857635218788815 66-67 22.951989369487347 26.921291838385397 26.06170319300543 24.06501559912183 68-69 23.259490377553536 26.778024130203587 26.031401179253933 23.93108431298894 70-71 23.248692199749357 26.719616667131536 26.092238028782667 23.939453104336444 72-73 23.17632541142152 26.80336158759185 26.142689555502063 23.877623445484566 74-75 23.030410477318505 26.77796805805979 26.09720262838261 24.09441883623909 76-77 23.273907345384952 26.836629909168447 25.925099426794834 23.964363318651767 78-79 23.13366538853029 26.637193673367758 26.148442193925693 24.08069874417626 80-81 23.147830880875038 26.435535154029182 26.244972731675475 24.171661233420302 82-83 23.027991380309455 26.55039946631037 26.34027801988333 24.081331133496846 84-85 23.09212326529762 26.437446563544764 26.183981419499492 24.286448751658128 86-87 23.074337551086703 26.63756770484677 26.243990006581537 24.04410473748499 88-89 23.139333754083268 26.28893857207757 26.246048415086427 24.325679258752743 90-91 23.05716589997724 26.2599325388239 26.19220908655769 24.490692474641172 92-93 22.984020635950195 26.236038677409557 26.248317517687152 24.531623168953093 94-95 23.014088531777215 26.08342952538154 26.284116844198962 24.61836509864228 96-97 22.894750201654983 26.108162279143137 26.37054878358331 24.62653873561857 98-99 22.79040943469749 26.168421744498854 26.280721686752646 24.760447134051013 100 23.022310469856095 25.94455412841282 26.19321977914737 24.839915622583717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 598.0 1 622.0 2 566.0 3 400.5 4 279.0 5 250.5 6 262.5 7 277.0 8 359.5 9 481.0 10 594.0 11 706.5 12 844.0 13 985.0 14 1171.0 15 1357.5 16 1549.0 17 1752.0 18 1889.5 19 2068.0 20 2269.0 21 2464.5 22 2668.0 23 2942.5 24 3309.5 25 3677.0 26 4154.5 27 4840.5 28 5701.5 29 6564.0 30 7409.5 31 8200.5 32 9013.5 33 9929.0 34 10811.0 35 11689.0 36 12673.5 37 13705.0 38 14827.0 39 15699.0 40 16286.0 41 16789.0 42 17004.0 43 17158.0 44 17099.0 45 16836.5 46 16490.0 47 16218.5 48 16206.0 49 16053.0 50 15875.0 51 15986.0 52 15854.0 53 15557.5 54 15283.5 55 14807.0 56 13882.5 57 12790.5 58 11749.0 59 10822.0 60 9994.0 61 8732.5 62 7451.5 63 6425.5 64 5399.0 65 4453.0 66 3655.0 67 2871.0 68 2158.5 69 1648.5 70 1258.5 71 896.0 72 622.5 73 442.5 74 310.5 75 215.5 76 158.0 77 112.5 78 75.5 79 56.0 80 29.0 81 22.5 82 20.5 83 10.5 84 9.0 85 9.0 86 4.0 87 3.5 88 2.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.29572065884323 2 25.766878045224917 3 6.758507005769457 4 23.069792303278707 5 12.95875781923115 6 10.66499208498502 7 8.888365582113375 8 9.426063293954497 9 5.913927249144487 10-11 7.362365645657769 12-13 10.624622705159618 14-15 8.114525687821343 16-17 8.542422424442913 18-19 7.176965530904068 20-21 8.74043797039205 22-23 6.174833055793847 24-25 5.835244337540346 26-27 10.432307465158049 28-29 6.86055182699478 30-31 4.567814016771982 32-33 9.468488521826565 34-35 7.747556811491816 36-37 7.766339786827247 38-39 6.451251170431672 40-41 8.7817418266023 42-43 7.070248327754509 44-45 6.9742776030306946 46-47 6.37630616436692 48-49 4.306721314845652 50-51 7.211728052420387 52-53 5.021035063422912 54-55 5.6042417752060985 56-57 5.361277915145806 58-59 3.905924393384655 60-61 4.533238390532633 62-63 3.352807821193551 64-65 5.048415221498938 66-67 2.952758480840431 68-69 5.716659284303226 70-71 2.840340971743303 72-73 1.781766122988306 74-75 0.9058814074709518 76-77 1.1733285488142429 78-79 1.858766977099722 80-81 2.36572041587937 82-83 0.8248623049046928 84-85 1.3042486903313466 86-87 0.4688268022778796 88-89 0.6482462681685571 90-91 0.23745044938969345 92-93 0.3029572439674877 94-95 0.5862904838531825 96-97 0.5999338390719527 98-99 0.47779777557241354 100 0.4139730384873444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 535059.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.58698826237288 #Duplication Level Percentage of deduplicated Percentage of total 1 96.22805970199464 91.01922353543485 2 2.842507571227626 5.377284605508271 3 0.5811874010681125 1.6491829764920567 4 0.18357113309288198 0.6945376244466768 5 0.06888599928351219 0.3257859602835697 6 0.03718775109262418 0.21104864256612524 7 0.020505148010461428 0.13576641359286137 8 0.012617965161059588 0.09547962580673386 9 0.0072110055787062526 0.061386057002969424 >10 0.016881937147953108 0.2368804497967815 >50 9.888482484450153E-4 0.06378890588443772 >100 1.9776904698655834E-4 0.02338297315747283 >500 1.977690469858612E-4 0.10625223002718202 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 568 0.10615651731865083 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.8689527696945572E-4 0.0 9 0.0 0.0 0.0 1.8689527696945572E-4 0.0 10-11 0.0 0.0 0.0 1.8689527696945572E-4 0.0 12-13 0.0 0.0 0.0 1.8689527696945572E-4 0.0 14-15 0.0 0.0 0.0 2.803429154541836E-4 0.0 16-17 0.0 0.0 0.0 3.7379055393891143E-4 0.0 18-19 0.0 0.0 0.0 4.672381924236392E-4 0.0 20-21 0.0 0.0 0.0 8.410287463625507E-4 0.0 22-23 0.0 0.0 0.0 0.0011213716618167341 0.0 24-25 0.0 0.0 0.0 0.0011213716618167341 0.0 26-27 0.0 0.0 0.0 0.0014017145772709178 0.0 28-29 0.0 0.0 0.0 0.0021492956851487405 0.0 30-31 0.0 0.0 0.0 0.005326515393629487 0.0 32-33 0.0 0.0 0.0 0.01728781311967465 0.0 34-35 0.0 0.0 0.0 0.03980869399449406 0.0 36-37 0.0 0.0 0.0 0.07541224425717538 0.0 38-39 0.0 0.0 0.0 0.12727568361619934 0.0 40-41 0.0 0.0 0.0 0.17465363632795636 0.0 42-43 0.0 0.0 0.0 0.22707776151788867 0.0 44-45 0.0 0.0 0.0 0.29529453761174 0.0 46-47 0.0 0.0 0.0 0.3780891453092089 0.0 48-49 0.0 0.0 0.0 0.46107064828364724 0.0 50-51 0.0 0.0 0.0 0.5435849130656619 0.0 52-53 0.0 0.0 0.0 0.6298370833870657 0.0 54-55 0.0 0.0 0.0 0.7346853337669303 0.0 56-57 0.0 0.0 0.0 0.8655120276455494 0.0 58-59 0.0 0.0 0.0 1.0011979987253743 0.0 60-61 0.0 0.0 0.0 1.1407153229830729 0.0 62-63 0.0 0.0 0.0 1.2840640004186454 0.0 64-65 0.0 0.0 0.0 1.4550731788456974 0.0 66-67 0.0 0.0 0.0 1.6589759260193735 0.0 68-69 0.0 0.0 0.0 1.8817550961669647 0.0 70-71 0.0 0.0 0.0 2.130606157451795 0.0 72-73 0.0 0.0 0.0 2.3914185164626707 0.0 74-75 0.0 0.0 0.0 2.6717614319168543 0.0 76-77 0.0 0.0 0.0 3.0006036717446114 0.0 78-79 0.0 0.0 0.0 3.36504946183505 0.0 80-81 0.0 0.0 0.0 3.762388820672113 0.0 82-83 0.0 0.0 0.0 4.180006317060362 0.0 84-85 0.0 0.0 0.0 4.6370587168891655 0.0 86-87 0.0 0.0 0.0 5.130462248088529 0.0 88 0.0 0.0 0.0 5.53527741800437 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 350 0.0 50.56326 1 GTATCAA 640 0.0 47.675495 1 CCGTGAT 65 0.0017142991 37.53825 2 TCAACGC 720 0.0 37.52196 4 TATCAAC 745 0.0 36.84543 2 ATCAACG 735 0.0 36.751675 3 CAACGCA 725 0.0 35.845997 5 AACGCAG 735 0.0 33.318398 6 GACCGTG 100 3.5613426E-5 29.906353 7 GAACCGC 70 0.006543189 28.558626 6 ACGCAGA 870 0.0 26.354258 7 TGGTATC 240 9.942596E-9 25.416525 2 GTATATA 170 1.1469181E-5 25.127789 1 GGACCGT 100 0.0012285898 24.988796 6 ATAATAC 180 1.4144043E-6 24.501116 3 GTCCTAT 125 0.0014079248 24.409851 1 CGCAGAG 955 0.0 24.078358 8 GTATAAA 275 4.1998646E-8 22.190773 1 GTGGTAT 220 3.3440592E-6 22.190773 1 GTACATG 1340 0.0 21.40416 1 >>END_MODULE