FastQCFastQC Report
Wed 25 May 2016
SRR1295117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535059
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.2756705335299472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.25585963417118485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.1312004844325579No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.067.375321
GTATCAA15050.044.4652751
TCAACGC20850.033.5720524
TATCAAC19850.033.43812
ATCAACG21300.032.8627853
CAACGCA21200.032.79625
AACGCAG22850.030.2082586
ACGCAGA26450.025.9167887
CGCAGAG27400.025.189578
CTTAGCG950.001307179224.7254413
ATAGGAC1151.3801728E-424.5104393
CTATTGG2152.5927875E-823.2281281
GAGTACT16800.022.49782812-13
GCAGAGT29400.022.198399
CAGAGTA27950.021.83813310-11
GTGTACA1654.2927146E-521.1868691
CTTAGAC1354.0858088E-420.8792653
GTATACA1705.4026204E-520.5637261
CTAAGAC1857.1968916E-620.314963
GTGGTAT3001.9863364E-919.976191
Produced by FastQC (version 0.11.5)