##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295117_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 535059 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.181948158988074 33.0 33.0 33.0 27.0 33.0 2 29.760168504781717 33.0 33.0 33.0 22.0 33.0 3 30.64001540017082 33.0 33.0 33.0 27.0 33.0 4 34.98597350946344 37.0 37.0 37.0 33.0 37.0 5 35.33300813555141 37.0 37.0 37.0 33.0 37.0 6 35.06767291083787 37.0 37.0 37.0 33.0 37.0 7 34.946673170622304 37.0 37.0 37.0 33.0 37.0 8 34.93374188640879 37.0 37.0 37.0 33.0 37.0 9 34.894006081572314 37.0 37.0 37.0 33.0 37.0 10-11 34.85553275433176 37.0 37.0 37.0 33.0 37.0 12-13 34.76529878013453 37.0 37.0 37.0 30.0 37.0 14-15 36.11940365455025 37.0 37.0 40.0 27.0 40.0 16-17 36.11202970139742 37.0 37.0 40.0 27.0 40.0 18-19 36.1524065570339 37.0 37.0 40.0 27.0 40.0 20-21 36.14835186400005 37.0 37.0 40.0 27.0 40.0 22-23 36.10456323508248 37.0 37.0 40.0 27.0 40.0 24-25 36.048798356816725 37.0 37.0 40.0 27.0 40.0 26-27 36.001993238128875 37.0 37.0 40.0 27.0 40.0 28-29 35.91950700763841 37.0 37.0 40.0 27.0 40.0 30-31 35.78308934154925 37.0 37.0 40.0 27.0 40.0 32-33 35.70940120622212 37.0 37.0 40.0 27.0 40.0 34-35 35.56241367774395 37.0 37.0 40.0 27.0 40.0 36-37 35.54435024922485 37.0 37.0 40.0 27.0 40.0 38-39 35.45978387430171 37.0 37.0 40.0 27.0 40.0 40-41 35.34021107952581 37.0 33.0 40.0 27.0 40.0 42-43 35.18380402908838 37.0 33.0 40.0 27.0 40.0 44-45 35.001046613551026 37.0 33.0 40.0 24.5 40.0 46-47 34.81207306110167 37.0 33.0 40.0 22.0 40.0 48-49 34.60936457474783 37.0 33.0 40.0 22.0 40.0 50-51 34.45682905997282 37.0 33.0 40.0 22.0 40.0 52-53 34.32675275063124 37.0 33.0 40.0 22.0 40.0 54-55 34.174679801666734 37.0 33.0 40.0 22.0 40.0 56-57 34.04088053840791 37.0 33.0 40.0 22.0 40.0 58-59 33.87687058810337 37.0 33.0 40.0 22.0 40.0 60-61 33.70075356175674 37.0 33.0 40.0 22.0 40.0 62-63 33.50419860239712 37.0 33.0 38.5 22.0 40.0 64-65 33.30109950491441 37.0 33.0 37.0 22.0 40.0 66-67 33.06827377915333 37.0 33.0 37.0 22.0 40.0 68-69 32.838945798500724 37.0 33.0 37.0 22.0 40.0 70-71 32.6347010329702 37.0 33.0 37.0 22.0 40.0 72-73 32.44522753565495 37.0 33.0 37.0 22.0 38.5 74-75 32.219206666928315 37.0 33.0 37.0 22.0 37.0 76-77 32.01519925839954 37.0 33.0 37.0 22.0 37.0 78-79 31.814593343911604 33.0 33.0 37.0 22.0 37.0 80-81 31.62347797158818 33.0 33.0 37.0 22.0 37.0 82-83 31.436996667657212 33.0 27.0 37.0 22.0 37.0 84-85 31.28261649649852 33.0 27.0 37.0 18.5 37.0 86-87 31.33439489850652 33.0 27.0 37.0 22.0 37.0 88-89 31.381544838980375 33.0 27.0 37.0 22.0 37.0 90-91 31.434745514046114 33.0 33.0 37.0 22.0 37.0 92-93 31.435051087823958 33.0 33.0 37.0 22.0 37.0 94-95 31.48565111511067 33.0 33.0 37.0 22.0 37.0 96-97 31.466320536613722 33.0 33.0 37.0 18.5 37.0 98-99 31.403969468787555 33.0 33.0 37.0 15.0 37.0 100 30.867016534625154 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6011789953957916 1101 2 4.085402043549486 1101 3 2.426236184662873 1101 4 1.099294816534794 1101 5 0.44662476969729425 1101 6 0.5633562837709789 1101 7 0.634700696687645 1101 8 0.6529630643823126 1101 9 0.6885083115242594 1101 10-11 0.7414745209965758 1101 12-13 0.8120028836217728 1101 14-15 1.2269759524295125 1101 16-17 1.151216668981931 1101 18-19 1.0758125020139602 1101 20-21 1.0807369156616247 1101 22-23 1.068654008471107 1101 24-25 1.0937873472052644 1101 26-27 0.9898955374489091 1101 28-29 0.9266468480675982 1101 30-31 0.6113421041280844 1101 32-33 0.8892871508552176 1101 34-35 1.008755914098053 1101 36-37 0.9319907824537665 1101 38-39 1.0756116611691482 1101 40-41 1.0396105078976419 1101 42-43 1.0718717697065756 1101 44-45 1.019617722486835 1101 46-47 0.9535411811057415 1101 48-49 0.9702845812463892 1101 50-51 0.8185769009978614 1101 52-53 0.8646172838890394 1101 54-55 0.8135914679440006 1101 56-57 0.7634295248456198 1101 58-59 0.8334480363319869 1101 60-61 0.9684852411307858 1101 62-63 0.8310529776123516 1101 64-65 0.9306158436705729 1101 66-67 0.7554105285366148 1101 68-69 0.7709993278243985 1101 70-71 0.7498099258644046 1101 72-73 0.6423588461956555 1101 74-75 0.6576905270647089 1101 76-77 0.5734519030704668 1101 78-79 0.6876652233639966 1101 80-81 0.8389329660294749 1101 82-83 0.821002859413241 1101 84-85 0.8716138721244171 1101 86-87 0.7800397912232242 1101 88-89 0.5357532225055941 1101 90-91 0.5317676481725684 1101 92-93 0.6520637685306951 1101 94-95 0.675242052772191 1101 96-97 0.6336428676228252 1101 98-99 0.44802792454198226 1101 100 0.7020605674334668 1102 1 0.6113606103720386 1102 2 4.03401199046203 1102 3 2.31271006004706 1102 4 1.0509258587253498 1102 5 0.6183291563974862 1102 6 0.7465345458553614 1102 7 0.6262611884451204 1102 8 0.5295577835776228 1102 9 0.5360097085625384 1102 10-11 0.7112509948719605 1102 12-13 0.7872829897966866 1102 14-15 0.9216469499148445 1102 16-17 0.9759501839302445 1102 18-19 0.9838783025168922 1102 20-21 0.9060547418700651 1102 22-23 0.9564156038888214 1102 24-25 0.9348690040926613 1102 26-27 1.0436130563089208 1102 28-29 0.8556043082520048 1102 30-31 0.9703519532479561 1102 32-33 1.0232966507155083 1102 34-35 1.1712883952380437 1102 36-37 1.10562412975748 1102 38-39 0.9771602781012518 1102 40-41 0.8600463838406327 1102 42-43 1.0726488722491894 1102 44-45 0.9981581667450072 1102 46-47 0.9963869480797598 1102 48-49 1.0322069059180876 1102 50-51 0.9163821538617896 1102 52-53 0.8612277197649831 1102 54-55 1.0504907414840972 1102 56-57 1.0626496282264526 1102 58-59 1.093449084110695 1102 60-61 0.9421374484512697 1102 62-63 1.1090244780314649 1102 64-65 0.958136449985183 1102 66-67 1.1633953008193743 1102 68-69 1.1408882632812265 1102 70-71 1.1649915408406528 1102 72-73 1.2005727327561502 1102 74-75 1.0119654641979778 1102 76-77 0.9974037751017555 1102 78-79 0.938387841413725 1102 80-81 0.9436696243139053 1102 82-83 1.1847441279239987 1102 84-85 0.8994463672347806 1102 86-87 0.8099007859144791 1102 88-89 0.898261255475699 1102 90-91 0.9242791737383662 1102 92-93 1.0231307565161707 1102 94-95 1.052815397281826 1102 96-97 0.9570656114060014 1102 98-99 0.9070426632958188 1102 100 0.9571206400794559 1103 1 0.5117254528264361 1103 2 4.059260746680938 1103 3 2.303870922401952 1103 4 1.1220908670172207 1103 5 0.4877984764638228 1103 6 0.4937733518255101 1103 7 0.6240804255927159 1103 8 0.7062534718031586 1103 9 0.6797451977831059 1103 10-11 0.5436556383213826 1103 12-13 0.669011845518078 1103 14-15 0.9084338487539796 1103 16-17 0.8828967013431779 1103 18-19 0.9048608895815704 1103 20-21 1.0137454549712217 1103 22-23 1.0405304463432188 1103 24-25 0.8819150239931588 1103 26-27 0.8101387611182034 1103 28-29 0.8753244575057408 1103 30-31 0.8516351207438078 1103 32-33 0.8407261697868194 1103 34-35 1.1180317617388695 1103 36-37 0.9166129357276347 1103 38-39 0.8131926164097152 1103 40-41 0.7275754053995129 1103 42-43 0.8893466334432105 1103 44-45 1.1529073375576075 1103 46-47 0.8924648916950204 1103 48-49 0.7082599739114528 1103 50-51 0.7450471621536678 1103 52-53 0.9059727446406001 1103 54-55 0.8209509404890696 1103 56-57 0.7470629781435321 1103 58-59 0.746444523580017 1103 60-61 0.7095980620499418 1103 62-63 0.8030211612818761 1103 64-65 0.8946434980780538 1103 66-67 0.7129516855623237 1103 68-69 0.8461909166808965 1103 70-71 0.8697262008738207 1103 72-73 0.724938404397939 1103 74-75 0.7915674542477333 1103 76-77 0.628077489861294 1103 78-79 0.8265864317951532 1103 80-81 0.7573636541646565 1103 82-83 0.7787573949223443 1103 84-85 1.0003377436858614 1103 86-87 0.8335616482693702 1103 88-89 0.8622309901357248 1103 90-91 0.88318671934368 1103 92-93 0.857245598970561 1103 94-95 0.9046479014277722 1103 96-97 0.9579611337940648 1103 98-99 0.9420613200122361 1103 100 0.9703544709252299 1104 1 0.384522122399531 1104 2 3.4715549457884904 1104 3 2.2344970016278154 1104 4 0.9547488827803292 1104 5 0.3395662697995476 1104 6 0.31352767300312223 1104 7 0.1870687485825755 1104 8 0.2670406014470359 1104 9 0.294540636397592 1104 10-11 0.2556839845626797 1104 12-13 0.4267245705527003 1104 14-15 0.42560743101450527 1104 16-17 0.38302492619828143 1104 18-19 0.44802778705297897 1104 20-21 0.42345164908655164 1104 22-23 0.5550796932359034 1104 24-25 0.4393148803813247 1104 26-27 0.48426597727112153 1104 28-29 0.4718457171867172 1104 30-31 0.3488184480933114 1104 32-33 0.3966471661794273 1104 34-35 0.41043358424148124 1104 36-37 0.2290648514069673 1104 38-39 0.3633028897851034 1104 40-41 0.2630188924317096 1104 42-43 0.375806088744028 1104 44-45 0.28659029732919095 1104 46-47 0.08485773845776379 1104 48-49 0.17102133890777083 1104 50-51 0.23749899234976368 1104 52-53 0.239857444850891 1104 54-55 0.38292201296176387 1104 56-57 0.2805061574360721 1104 58-59 0.33393447929969966 1104 60-61 0.22663487113167946 1104 62-63 0.33388702098678635 1104 64-65 0.33007373520855054 1104 66-67 0.39828361697058057 1104 68-69 0.4045738302915396 1104 70-71 0.4910568329368772 1104 72-73 0.3317497087011603 1104 74-75 0.37433659821859777 1104 76-77 0.3100713008749558 1104 78-79 0.30221516100135304 1104 80-81 0.48800811228641905 1104 82-83 0.3365654337659194 1104 84-85 0.6107436180938883 1104 86-87 0.5369540505192916 1104 88-89 0.41777586028669234 1104 90-91 0.5134930912641416 1104 92-93 0.649715808062556 1104 94-95 0.5722655691031378 1104 96-97 0.2960003193097833 1104 98-99 0.32938372858792064 1104 100 0.25114125863615655 1105 1 0.35023763036656064 1105 2 3.655300678492708 1105 3 2.1391658255907444 1105 4 1.0435340482624582 1105 5 0.3894685702567031 1105 6 0.15257261761652074 1105 7 0.09343319611425471 1105 8 0.2556074959851813 1105 9 0.12870554531959044 1105 10-11 0.29964595515681225 1105 12-13 0.3131259095447021 1105 14-15 0.4368626442451671 1105 16-17 0.47077591660622176 1105 18-19 0.2706469960255191 1105 20-21 0.14631425981035306 1105 22-23 0.04612013689347805 1105 24-25 0.26407161981438065 1105 26-27 0.26599185663759783 1105 28-29 0.5362811776028664 1105 30-31 0.5734836295010339 1105 32-33 0.5881336816659299 1105 34-35 0.7474500632741936 1105 36-37 0.5210435353997767 1105 38-39 0.7803422830314162 1105 40-41 0.4739987256647993 1105 42-43 0.625502542476525 1105 44-45 0.70285105910326 1105 46-47 0.5424551485727775 1105 48-49 0.7276930740911851 1105 50-51 0.5932912198711051 1105 52-53 0.6351749260098458 1105 54-55 0.7144834831986984 1105 56-57 0.5767337832524717 1105 58-59 0.5366444415470184 1105 60-61 0.554021521308016 1105 62-63 0.6354994164045067 1105 64-65 0.48433030098490804 1105 66-67 0.48454087737373897 1105 68-69 0.5698960693617607 1105 70-71 0.5699449782550658 1105 72-73 0.3818306073110662 1105 74-75 0.4455316104592768 1105 76-77 0.3606703459042784 1105 78-79 0.36694816735182556 1105 80-81 0.3712038615091977 1105 82-83 0.40707499617926857 1105 84-85 0.4109466411323446 1105 86-87 0.5514914460076064 1105 88-89 0.6617928221697547 1105 90-91 0.584923175381757 1105 92-93 0.5967438077350096 1105 94-95 0.38584333483318645 1105 96-97 0.4015821822085215 1105 98-99 0.5613774345913569 1105 100 0.6083559180854827 1106 1 0.3018065412527555 1106 2 3.8158124363929105 1106 3 2.1840367044171813 1106 4 0.8996856134633617 1106 5 0.4418631586271431 1106 6 0.4242547242277084 1106 7 0.2907706644763337 1106 8 0.30319490732343723 1106 9 0.3864305558312111 1106 10-11 0.10108934492770061 1106 12-13 0.06857340228274467 1106 14-15 0.12673892315899593 1106 16-17 0.2723395373304598 1106 18-19 0.20463917208211058 1106 20-21 0.20461940184776495 1106 22-23 0.07754575996462876 1106 24-25 0.16806995169578443 1106 26-27 0.08490068172698528 1106 28-29 0.08900313768344148 1106 30-31 0.01309024756233157 1106 32-33 0.08122864625619997 1106 34-35 0.24610862935175248 1106 36-37 0.2926071286426506 1106 38-39 0.1367171342885456 1106 40-41 0.2533072534058576 1106 42-43 0.16005829253904125 1106 44-45 0.21965900286540574 1106 46-47 0.0743300918924703 1106 48-49 -0.09840624904289541 1106 50-51 0.006476914173944692 1106 52-53 0.047285412072675115 1106 54-55 -0.09230468772437206 1106 56-57 -0.04083560031313027 1106 58-59 0.01721580651872756 1106 60-61 -0.010235739954524092 1106 62-63 0.14360641783078876 1106 64-65 -0.05074175514302226 1106 66-67 -0.10361417521183824 1106 68-69 -0.3254411681568996 1106 70-71 -0.12397724399769317 1106 72-73 -0.12990943000862387 1106 74-75 -0.27632885018329034 1106 76-77 -0.03999449691607637 1106 78-79 -0.1855906981626383 1106 80-81 -0.10695189185108234 1106 82-83 0.06663933416702861 1106 84-85 -0.19805920690122747 1106 86-87 -0.04031381832186298 1106 88-89 -0.057172133598999864 1106 90-91 -0.029562799505775672 1106 92-93 0.15025310880156084 1106 94-95 0.09458797253266482 1106 96-97 0.07742235611057424 1106 98-99 0.16875810365256783 1106 100 0.1433190793213761 1107 1 0.4837365894843124 1107 2 3.3835985875029344 1107 3 2.147879772840792 1107 4 0.9142744226940138 1107 5 0.4752221067891327 1107 6 0.10994185656031874 1107 7 0.20272332943729054 1107 8 0.2233719698265304 1107 9 0.1731441733144976 1107 10-11 0.31331365805733924 1107 12-13 0.3283164971944217 1107 14-15 0.28017392989743684 1107 16-17 0.037213238760529066 1107 18-19 0.038715117138302446 1107 20-21 0.2770838976577039 1107 22-23 0.24317691807507202 1107 24-25 0.1803705271561924 1107 26-27 0.14465309112180336 1107 28-29 0.30804012200309927 1107 30-31 0.3667542596013078 1107 32-33 0.3520869031088907 1107 34-35 0.5289330862737174 1107 36-37 0.40049249355208616 1107 38-39 0.19611806748332583 1107 40-41 0.20074264145595322 1107 42-43 0.12881184004726975 1107 44-45 0.36253262279374354 1107 46-47 0.21249225704407593 1107 48-49 0.05116251949510797 1107 50-51 0.010468315741690049 1107 52-53 -0.044500087023529034 1107 54-55 0.11903947951716276 1107 56-57 -0.016045976647355076 1107 58-59 -0.08070779143325524 1107 60-61 0.22804802286380266 1107 62-63 0.19722291145182425 1107 64-65 -0.025878998230659533 1107 66-67 0.2882843347619328 1107 68-69 0.05532908138393111 1107 70-71 0.12192365572316533 1107 72-73 0.2864974499014963 1107 74-75 0.2700594589760499 1107 76-77 0.08636265238635588 1107 78-79 0.11273618780188954 1107 80-81 0.31100861474036634 1107 82-83 0.4234473481154666 1107 84-85 0.3154711714123266 1107 86-87 0.30376719148454256 1107 88-89 0.2562649543442781 1107 90-91 0.30198913892548873 1107 92-93 0.3035822360635976 1107 94-95 0.17998249910950648 1107 96-97 0.16861478460008783 1107 98-99 0.22908248052819857 1107 100 0.22808669752070898 1108 1 0.5200423903888343 1108 2 3.5401622843327267 1108 3 2.067529539476112 1108 4 1.0062260266514613 1108 5 0.18980690618757734 1108 6 0.19468316046493328 1108 7 -0.05808389971608818 1108 8 0.006348295752260924 1108 9 -0.06476065247860419 1108 10-11 0.07853059184928668 1108 12-13 0.13631951372615703 1108 14-15 0.19108649651434462 1108 16-17 -0.002778825305689736 1108 18-19 0.08038753845308122 1108 20-21 0.22002409535453182 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0.14882595638113116 2210 70-71 0.3421473939130877 2210 72-73 0.31829243752786596 2210 74-75 -0.11887902601829126 2210 76-77 0.3087353061693925 2210 78-79 0.2537229770127638 2210 80-81 0.4687923500262343 2210 82-83 0.26815985054672353 2210 84-85 0.3503432089786891 2210 86-87 0.4551951559797054 2210 88-89 0.41141307490583756 2210 90-91 0.3175451366631883 2210 92-93 0.4056689473846866 2210 94-95 0.18112126751869795 2210 96-97 0.3764236615261538 2210 98-99 0.20961003370433673 2210 100 0.3280715497945259 2211 1 0.6308402372743487 2211 2 4.172585873623543 2211 3 2.4874425097279627 2211 4 1.3008460845476364 2211 5 0.5326197798633672 2211 6 0.43809811973851964 2211 7 0.6179720377332885 2211 8 0.6435747202491982 2211 9 0.5296903663926855 2211 10-11 0.5463074504438765 2211 12-13 0.5330696859184272 2211 14-15 0.4967905434347344 2211 16-17 0.5885968456877251 2211 18-19 0.5527774188644656 2211 20-21 0.6241757531611754 2211 22-23 0.491996728091074 2211 24-25 0.5840743305531433 2211 26-27 0.6831491387831505 2211 28-29 0.6249507736422899 2211 30-31 0.7066976822612077 2211 32-33 0.8682352859634292 2211 34-35 0.8942946544427528 2211 36-37 0.5732597458595947 2211 38-39 0.4994099099127425 2211 40-41 0.5907680333251761 2211 42-43 0.6285061378358279 2211 44-45 1.036713026833361 2211 46-47 0.9251866535289466 2211 48-49 0.9434194734193113 2211 50-51 0.7044847557272789 2211 52-53 0.6937111512819172 2211 54-55 0.8829220129617639 2211 56-57 0.4449023282755391 2211 58-59 0.6878250045819811 2211 60-61 0.47233933848563225 2211 62-63 0.9722645369416298 2211 64-65 0.831055571241933 2211 66-67 0.8186321687624272 2211 68-69 0.7026224311751932 2211 70-71 0.669750991012485 2211 72-73 0.4628248191577171 2211 74-75 0.7491525039623426 2211 76-77 0.41733688752198717 2211 78-79 0.5552833986056775 2211 80-81 0.5544661387959913 2211 82-83 0.2871054435842808 2211 84-85 0.649526141412494 2211 86-87 0.42484064845743674 2211 88-89 0.6092952515483532 2211 90-91 0.8224032532670869 2211 92-93 0.7885842420340801 2211 94-95 0.7025429377825674 2211 96-97 0.5775418018821945 2211 98-99 0.5155030216659746 2211 100 0.263168750045093 2212 1 0.6323129913244223 2212 2 4.382821514271551 2212 3 2.2669957743327735 2212 4 0.9931877634872492 2212 5 0.5483291563974859 2212 6 0.5772488315696478 2212 7 0.4664992836832198 2212 8 0.3882482597680976 2212 9 0.14589066094349334 2212 10-11 0.0364890901100523 2212 12-13 0.15406870408239826 2212 14-15 0.42533742610532244 2212 16-17 0.34958113631119403 2212 18-19 0.45256877870736645 2212 20-21 0.7126023609176784 2212 22-23 0.3849275086507262 2212 24-25 0.38516662314028594 2212 26-27 0.459327342023208 2212 28-29 0.055068593966289825 2212 30-31 0.11064957229557137 2212 32-33 0.5730585554774095 2212 34-35 0.335752680952325 2212 36-37 0.4227074630908163 2212 38-39 0.3470412304822048 2212 40-41 0.7012368600311163 2212 42-43 0.3443750627253763 2212 44-45 0.32655102388785906 2212 46-47 0.555672662365474 2212 48-49 0.3391116678228485 2212 50-51 0.2149535824332176 2212 52-53 0.04902533881260496 2212 54-55 0.13203836053171614 2212 56-57 0.4297329615597789 2212 58-59 0.4241038460154627 2212 60-61 0.38267316273697816 2212 62-63 0.3190244780314586 2212 64-65 0.22105311665185212 2212 66-67 0.1111333960574683 2212 68-69 0.3436263585193302 2212 70-71 0.7249915408406551 2212 72-73 0.04569178037520061 2212 74-75 0.14291784515035744 2212 76-77 0.2711537751017623 2212 78-79 0.10797117474706042 2212 80-81 0.0330743862186722 2212 82-83 0.2135536517335197 2212 84-85 0.47504160533001993 2212 86-87 0.4538293573430501 2212 88-89 0.6133207792852211 2212 90-91 0.601600602309798 2212 92-93 0.7278331374685472 2212 94-95 0.36757730204373473 2212 96-97 0.007303706644101737 2212 98-99 0.2779355204386782 2212 100 0.27426349722231436 2213 1 -0.9341466360047725 2213 2 -25.724321342871303 2213 3 -14.65960878053265 2213 4 -6.421719068810884 2213 5 -2.6792070754865662 2213 6 -1.4135379179127554 2213 7 -1.6816373622795169 2213 8 -1.353159607726731 2213 9 -1.5919975378142723 2213 10-11 -1.5884591500555771 2213 12-13 -1.8392025174496993 2213 14-15 -2.2280269631286345 2213 16-17 -2.261857787084253 2213 18-19 -2.022534740961369 2213 20-21 -2.119126417550227 2213 22-23 -1.9734575845169786 2213 24-25 -1.9030838944580637 2213 26-27 -2.374213030647603 2213 28-29 -2.4480369960958157 2213 30-31 -2.226876307621609 2213 32-33 -2.1972830594294166 2213 34-35 -1.593439865631531 2213 36-37 -1.9370751456048367 2213 38-39 -2.354252765377005 2213 40-41 -2.2445188335506643 2213 42-43 -1.6986373596348727 2213 44-45 -1.84146139847239 2213 46-47 -1.9466565301811087 2213 48-49 -2.0361988911833677 2213 50-51 -1.5621685707758957 2213 52-53 -2.033065758495887 2213 54-55 -2.002588968660831 2213 56-57 -1.7539626906141308 2213 58-59 -1.8475835245849552 2213 60-61 -2.036322696476269 2213 62-63 -1.833149435012018 2213 64-65 -1.7416280427684399 2213 66-67 -1.550771365847293 2213 68-69 -1.9537856497622457 2213 70-71 -2.1235591837970276 2213 72-73 -1.956999731011969 2213 74-75 -1.8945562749324552 2213 76-77 -2.143338978521431 2213 78-79 -1.8409418687312034 2213 80-81 -1.9118013901788444 2213 82-83 -1.8225747126557117 2213 84-85 -1.5822565313159416 2213 86-87 -1.2661499387232027 2213 88-89 -1.6315032372779257 2213 90-91 -1.6204490871311954 2213 92-93 -1.8777569246432542 2213 94-95 -2.1458802548920843 2213 96-97 -2.0154706204780553 2213 98-99 -1.9252218294578043 2213 100 -1.9988213889060518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 12.0 5 8.0 6 8.0 7 9.0 8 12.0 9 21.0 10 102.0 11 425.0 12 1244.0 13 2170.0 14 2855.0 15 3442.0 16 3820.0 17 3819.0 18 3961.0 19 4230.0 20 4771.0 21 5630.0 22 6442.0 23 6918.0 24 6898.0 25 6865.0 26 7026.0 27 7650.0 28 8848.0 29 10597.0 30 12671.0 31 15493.0 32 19184.0 33 25826.0 34 34786.0 35 49196.0 36 74239.0 37 117636.0 38 88003.0 39 240.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.42574903734952 19.55279437483846 10.490201812292117 30.531254775519905 2 14.526366363504609 22.11613866437955 34.19596538353872 29.161529588577125 3 16.438374085848476 25.422430049770213 28.65048527358665 29.48871059079466 4 12.20464052214285 18.27126158354921 33.69574562731531 35.82835226699263 5 13.02219534977752 37.39421314798834 32.86660139907266 16.716990103161486 6 31.54196126537352 36.74815386888121 16.77609126965576 14.933793596089512 7 27.39879443285245 32.88573287563663 20.99122747975141 18.724245211759516 8 26.148107289436535 34.0854314108617 19.85789478604751 19.908566513654254 9 26.292289454888127 15.961639860440592 20.8088238043775 36.93724688029378 10-11 24.74907633165303 26.379145288917478 27.403500964803374 21.46827741462612 12-13 25.200274854976605 24.555487517003147 27.65265436051811 22.591583267502138 14-15 23.13002570054795 25.948671826720904 25.476638117773764 25.444664354957382 16-17 21.862651708416962 28.517209434503748 26.571490269067137 23.04864858801215 18-19 21.98160184187231 27.18790107507985 29.01285403274128 21.81764305030656 20-21 23.29974304881138 26.236314708656238 28.821716628359223 21.642225614173164 22-23 23.14690445812244 25.977501413756197 28.574887016343485 22.300707111777875 24-25 22.890238567919514 26.19218532102546 28.586930919613717 22.330645191441313 26-27 22.53113188854952 26.138169134662476 28.843400338702985 22.487298638085022 28-29 22.47325013645982 26.077471792072622 28.870505237813955 22.57877283365361 30-31 22.795186353545972 26.32905713284262 28.46640962729291 22.409346886318495 32-33 22.519764507989905 26.25380805532193 28.769928044108028 22.45649939258013 34-35 22.696187366481198 25.971381418481336 28.593723424735114 22.738707790302353 36-37 22.799357801423078 26.238372299485828 28.316079074905097 22.64619082418599 38-39 22.705565928941375 26.2339189156354 28.512699454042163 22.54781570138106 40-41 22.769416239503943 26.124789144381776 28.406468886822516 22.699325729291758 42-43 22.779131839047803 26.10678932966491 28.281116826412667 22.83296200487462 44-45 22.84761837117672 26.079321585310232 28.09572495570193 22.977335087811117 46-47 23.018608694026714 26.42686904750778 27.617040367126823 22.937481891338685 48-49 23.028476087657555 26.32250466189027 27.47388640410527 23.1751328463469 50-51 23.012862033866938 26.304686543420697 27.358717180129226 23.323734242583136 52-53 23.08296301558606 26.287387759604115 27.267569138962163 23.362080085847662 54-55 23.15758874157824 26.341125094405932 27.164513089709942 23.33677307430588 56-57 22.88180898849101 26.25197907184101 27.323995790441764 23.54221614922622 58-59 22.7167285383698 26.074249782234038 27.478232749757936 23.730788929638226 60-61 22.79990803549232 26.34792491060564 27.358693356311996 23.493473697590044 62-63 22.808586762075134 26.23200109166428 27.337124249714467 23.622287896546116 64-65 22.881585637589914 25.99990279501712 27.391149112443735 23.72736245494923 66-67 22.65656472189275 26.1056946816177 27.435360358465754 23.8023802380238 68-69 22.674701745825658 26.01604585491429 27.371708407762508 23.93754399149755 70-71 22.67387677078319 25.943381675928567 27.37225356259844 24.010487990689803 72-73 22.726898838889625 26.169195710004693 27.22384136663682 23.880064084468863 74-75 22.62423982965454 26.157244262151043 27.203863058104776 24.01465285008964 76-77 22.74866367139635 26.088947751631213 26.973041632512672 24.189346944459764 78-79 22.664852239792214 26.146906891301686 27.123687897392667 24.064552971513436 80-81 22.546745349765317 26.041314234550157 27.16613735646472 24.24580305921981 82-83 22.372865198886736 26.00396633289907 27.369192322286047 24.253976145928156 84-85 22.4585411261561 25.93095546068323 27.08556079762537 24.524942615535302 86-87 22.309076210636498 26.085916289328452 27.221715989327055 24.383291510707995 88-89 22.28722613855242 25.79734416427341 27.19554102209209 24.71988867508208 90-91 22.161621636776538 25.79376279169743 27.338112015550962 24.706503555975065 92-93 22.01146836573919 25.827933395260516 27.368013800914703 24.79258443808559 94-95 21.840190039024538 25.650502384832762 27.51715733915462 24.992150236988085 96-97 21.780914342744897 25.68138364380218 27.4244885067031 25.113213506749826 98-99 21.720136097776592 25.752607434915753 27.30449961826461 25.222756849043044 100 21.871433649510767 25.48109483452134 27.307627546725037 25.33984396924286 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 93.0 1 69.5 2 38.0 3 27.0 4 24.5 5 57.0 6 171.0 7 391.0 8 597.0 9 659.0 10 470.0 11 251.0 12 217.0 13 241.5 14 281.5 15 325.5 16 340.0 17 366.0 18 431.5 19 483.0 20 547.5 21 630.0 22 751.5 23 922.0 24 1172.0 25 1518.5 26 2007.5 27 2786.5 28 3671.5 29 4592.5 30 5580.0 31 6475.5 32 7579.5 33 8636.5 34 9635.5 35 10991.5 36 12332.0 37 13631.5 38 15162.5 39 16446.5 40 17193.5 41 17752.0 42 18235.0 43 18484.0 44 18372.0 45 18135.0 46 17939.5 47 17778.0 48 17677.5 49 17765.0 50 17753.5 51 17711.5 52 17839.5 53 17877.0 54 17468.5 55 16778.5 56 15939.0 57 14696.0 58 13455.0 59 12583.5 60 11549.0 61 10076.0 62 8718.5 63 7404.5 64 6158.0 65 5168.0 66 4079.0 67 2985.5 68 2216.0 69 1606.5 70 1081.5 71 703.5 72 468.5 73 319.5 74 209.0 75 127.5 76 81.0 77 56.5 78 29.5 79 12.0 80 4.0 81 4.0 82 3.0 83 1.0 84 1.5 85 2.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.936719128170912 2 5.862531047977886 3 0.0 4 0.0061675441399920376 5 0.15848719487009844 6 0.05457342087508107 7 0.038687322332677326 8 0.04560244758054719 9 0.04354659953388318 10-11 0.043920390087822093 12-13 0.04326625661842899 14-15 0.04588279049600137 16-17 0.035977340816620226 18-19 0.03149185416935329 20-21 0.02476362419845288 22-23 0.025511205306330706 24-25 0.02186674740542632 26-27 0.014390936326648089 28-29 0.019250213527853936 30-31 0.02270777615178887 32-33 0.0016820574927251014 34-35 0.004111696093328026 36-37 0.004111696093328026 38-39 0.006728229970900406 40-41 0.0068216776093851334 42-43 0.008036496909686596 44-45 0.008784078017564418 46-47 0.017568156035128835 48-49 0.025511205306330706 50-51 0.021025718659063768 52-53 0.029529453761174 54-55 0.025230862390876522 56-57 0.01607299381937319 58-59 0.015138517434525912 60-61 0.013082669387861899 62-63 0.01794194658906775 64-65 0.019997794635731758 66-67 0.021025718659063768 68-69 0.028875320291780908 70-71 0.030183587230567098 72-73 0.026165338775723797 74-75 0.027286710437540534 76-77 0.019530556443308124 78-79 0.016353336734827372 80-81 0.01607299381937319 82-83 0.010466135510289519 84-85 0.013269564664831354 86-87 0.011494059533621526 88-89 0.009064420933018602 90-91 0.008223392186656051 92-93 0.004111696093328026 94-95 0.002055848046664013 96-97 0.0014951622157556455 98-99 4.672381924236393E-4 100 0.10260550705623117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 535059.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.92394829556972 #Duplication Level Percentage of deduplicated Percentage of total 1 80.67148143025868 58.82882940747196 2 12.203035069711722 17.797869969453632 3 3.8607953905953494 8.446333303306474 4 1.5239517171122556 4.4453030489455525 5 0.704542200080176 2.568899948534684 6 0.3493504345772512 1.5285607816887765 7 0.21188267994027402 1.0815925117683902 8 0.12288848859752712 0.7169211030885421 9 0.07807266687662447 0.5124030410347381 >10 0.26602789054849424 2.9010191993731045 >50 0.005400410217706647 0.2805492587663158 >100 0.0018001350387325823 0.226727145682641 >500 2.5716214839013635E-4 0.1316480200737151 >1k 5.143242967802727E-4 0.5333432608114611 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1475 0.2756705335299472 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1369 0.25585963417118485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 702 0.1312004844325579 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.7379055393891143E-4 0.0 8 0.0 0.0 0.0 3.7379055393891143E-4 0.0 9 0.0 0.0 0.0 3.7379055393891143E-4 0.0 10-11 0.0 0.0 0.0 3.7379055393891143E-4 0.0 12-13 0.0 0.0 0.0 4.672381924236392E-4 0.0 14-15 0.0 0.0 0.0 5.606858309083671E-4 0.0 16-17 0.0 0.0 0.0 5.606858309083671E-4 0.0 18-19 0.0 0.0 0.0 6.54133469393095E-4 0.0 20-21 0.0 0.0 0.0 8.410287463625507E-4 0.0 22-23 0.0 0.0 0.0 0.0011213716618167341 0.0 24-25 0.0 0.0 0.0 0.0014017145772709178 0.0 26-27 0.0 0.0 0.0 0.001962400408179285 0.0 28-29 0.0 0.0 0.0 0.0034575626239349306 0.0 30-31 0.0 0.0 0.0 0.009531659125442241 0.0 32-33 0.0 0.0 0.0 0.02906221556875036 0.0 34-35 0.0 0.0 0.0 0.0654133469393095 0.0 36-37 0.0 0.0 0.0 0.12307053988438658 0.0 38-39 0.0 0.0 0.0 0.19866967941853142 0.0 40-41 0.0 0.0 0.0 0.2669799031508675 0.0 42-43 0.0 0.0 0.0 0.3455693671165236 0.0 44-45 0.0 0.0 0.0 0.45116519860426607 0.0 46-47 0.0 0.0 0.0 0.5775064058356181 0.0 48-49 0.0 0.0 0.0 0.6991752311427337 0.0 50-51 0.0 0.0 0.0 0.8231802474119676 0.0 52-53 0.0 0.0 0.0 0.9512969597745295 0.0 54-55 0.0 0.0 0.0 1.102588686481304 0.0 56-57 0.0 0.0 0.0 1.289670858727729 0.0 58-59 0.0 0.0 0.0 1.4903029385544397 0.0 60-61 0.0 0.0 0.0 1.690093989634788 0.0 62-63 0.0 0.0 0.0 1.9006315191408798 0.0 64-65 0.0 0.0 0.0 2.1442495126705654 0.0 66-67 0.0 0.0 0.0 2.4311337628186798 0.0 68-69 0.0 0.0 0.0 2.7470802285355447 0.0 70-71 0.0 0.0 0.0 3.089378928305103 0.0 72-73 0.0 0.0 0.0 3.4375648292991987 0.0 74-75 0.0 0.0 0.0 3.8086454017220532 0.0 76-77 0.0 0.0 0.0 4.232991128081203 0.0 78-79 0.0 0.0 0.0 4.7180778194554245 0.0 80-81 0.0 0.0 0.0 5.22951674488234 0.0 82-83 0.0 0.0 0.0 5.765065161038315 0.0 84-85 0.0 0.0 0.0 6.342571566873934 0.0 86-87 0.0 0.0 0.0 6.959045637957683 0.0 88 0.0 0.0 0.0 7.46160703772855 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 845 0.0 67.37532 1 GTATCAA 1505 0.0 44.465275 1 TCAACGC 2085 0.0 33.572052 4 TATCAAC 1985 0.0 33.4381 2 ATCAACG 2130 0.0 32.862785 3 CAACGCA 2120 0.0 32.7962 5 AACGCAG 2285 0.0 30.208258 6 ACGCAGA 2645 0.0 25.916788 7 CGCAGAG 2740 0.0 25.18957 8 CTTAGCG 95 0.0013071792 24.725441 3 ATAGGAC 115 1.3801728E-4 24.510439 3 CTATTGG 215 2.5927875E-8 23.228128 1 GAGTACT 1680 0.0 22.497828 12-13 GCAGAGT 2940 0.0 22.19839 9 CAGAGTA 2795 0.0 21.838133 10-11 GTGTACA 165 4.2927146E-5 21.186869 1 CTTAGAC 135 4.0858088E-4 20.879265 3 GTATACA 170 5.4026204E-5 20.563726 1 CTAAGAC 185 7.1968916E-6 20.31496 3 GTGGTAT 300 1.9863364E-9 19.97619 1 >>END_MODULE