FastQCFastQC Report
Wed 25 May 2016
SRR1295116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752926
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG1454.481894E-420.5991675
CCGTCCG1250.00456695219.9373869
TCTATAC8900.019.1475163
GTCGTAC1650.007241887518.4079861
GTATATA16400.018.1498221
AGTACCG2252.366504E-517.7000275
GTATTAT13400.017.6799071
CTATACC7900.016.700724
GTATTAG10000.016.4015141
CTTACAC11650.016.04333
GTACATG102150.015.8778991
TACATGG102850.015.76572
GTGTATA15250.015.53513051
GTATTAA12750.015.2461411
CATGGGG36000.014.6595214
ACATGGG105200.014.2655213
CTACGCT2350.00583799214.035714
TACACTG16350.014.00575355
TAAGCGC2500.001051430913.9411686
CTCTATA9650.013.8452772
Produced by FastQC (version 0.11.5)