##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2752926 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.497714431844518 33.0 27.0 33.0 6.0 33.0 2 22.67580022129182 27.0 6.0 33.0 2.0 33.0 3 24.96296849243314 33.0 15.0 33.0 15.0 33.0 4 25.61531548614093 33.0 15.0 37.0 2.0 37.0 5 27.154091319563257 33.0 15.0 37.0 2.0 37.0 6 27.960089373996976 33.0 15.0 37.0 6.0 37.0 7 27.768292718365842 33.0 15.0 37.0 6.0 37.0 8 28.622094818385964 33.0 22.0 37.0 6.0 37.0 9 29.54034834209129 37.0 27.0 37.0 15.0 37.0 10-11 29.05628048120436 35.0 24.5 37.0 10.5 37.0 12-13 27.90466852359998 33.0 15.0 37.0 4.0 37.0 14-15 29.672114869778554 33.0 27.0 37.0 10.5 40.0 16-17 30.06877518683757 37.0 27.0 38.5 6.0 40.0 18-19 30.163797900851677 37.0 27.0 40.0 10.5 40.0 20-21 29.671613766588713 33.0 27.0 40.0 6.0 40.0 22-23 30.402912755373737 37.0 27.0 40.0 15.0 40.0 24-25 30.522824623691303 37.0 27.0 40.0 15.0 40.0 26-27 29.07158546942417 33.0 22.0 38.5 4.0 40.0 28-29 29.795805444824886 33.0 27.0 40.0 10.5 40.0 30-31 30.487689462048742 37.0 27.0 40.0 15.0 40.0 32-33 29.22763670363824 33.0 24.5 37.0 6.0 40.0 34-35 28.904638555486052 33.0 22.0 37.0 6.0 40.0 36-37 29.221146699911294 33.0 22.0 37.0 6.0 40.0 38-39 29.31784835480503 33.0 24.5 37.0 6.0 40.0 40-41 28.45088898139652 33.0 22.0 37.0 6.0 40.0 42-43 29.145584189331643 33.0 22.0 37.0 6.0 40.0 44-45 28.96053217558336 33.0 22.0 37.0 6.0 40.0 46-47 28.601550858976957 33.0 22.0 37.0 6.0 40.0 48-49 28.493984582222694 33.0 22.0 37.0 6.0 40.0 50-51 28.43573129099729 33.0 22.0 37.0 6.0 40.0 52-53 28.653596754870996 33.0 22.0 37.0 6.0 40.0 54-55 28.60729856160318 33.0 22.0 37.0 6.0 40.0 56-57 28.5454868746926 33.0 22.0 37.0 6.0 40.0 58-59 28.458297825658953 33.0 22.0 37.0 6.0 40.0 60-61 28.434461551091456 33.0 22.0 37.0 10.5 40.0 62-63 28.345907590687148 33.0 22.0 37.0 6.0 40.0 64-65 27.882928382382964 33.0 22.0 37.0 6.0 40.0 66-67 28.124479372129873 33.0 22.0 37.0 10.5 40.0 68-69 27.725946320387834 33.0 22.0 37.0 6.0 37.0 70-71 27.869622539799472 33.0 22.0 37.0 10.5 37.0 72-73 27.870434039999623 33.0 22.0 37.0 10.5 37.0 74-75 27.959809126725528 33.0 22.0 37.0 15.0 37.0 76-77 27.710259556559095 33.0 22.0 37.0 15.0 37.0 78-79 27.254127971474716 33.0 22.0 37.0 6.0 37.0 80-81 27.090498436935828 27.0 22.0 33.0 6.0 37.0 82-83 27.20632501563791 27.0 22.0 33.0 15.0 37.0 84-85 27.139302872652586 27.0 22.0 33.0 10.5 37.0 86-87 26.90953589017649 27.0 22.0 33.0 15.0 37.0 88-89 26.83746947793003 27.0 22.0 33.0 15.0 37.0 90-91 27.00726481569065 27.0 22.0 33.0 15.0 37.0 92-93 26.964816889375157 27.0 22.0 33.0 15.0 37.0 94-95 26.853582697101196 27.0 22.0 33.0 15.0 37.0 96-97 26.91892480945728 27.0 22.0 33.0 15.0 37.0 98-99 26.96957818699086 27.0 22.0 33.0 15.0 37.0 100 26.632969429617795 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.280580484989223 1101 2 11.242536257632722 1101 3 8.493309899342535 1101 4 12.952619433993775 1101 5 10.705543181642842 1101 6 9.998185313758565 1101 7 9.475398617695138 1101 8 9.004599966901587 1101 9 7.329676880625648 1101 10-11 7.796433157465305 1101 12-13 9.05483162850966 1101 14-15 7.824357316453391 1101 16-17 8.097441065463862 1101 18-19 8.16733118610513 1101 20-21 7.269016002830849 1101 22-23 7.480648070691224 1101 24-25 7.425738512767737 1101 26-27 8.624308240854464 1101 28-29 8.319009960538597 1101 30-31 5.616416460664407 1101 32-33 7.039154407758506 1101 34-35 8.741309857327963 1101 36-37 8.105331135244644 1101 38-39 8.22442430661701 1101 40-41 9.13390700701746 1101 42-43 8.67993606603741 1101 44-45 8.550460258355582 1101 46-47 7.409212139965078 1101 48-49 5.289817960739615 1101 50-51 6.819647669363693 1101 52-53 5.799710157962416 1101 54-55 3.8955722593537416 1101 56-57 6.149227567130591 1101 58-59 6.230658368002757 1101 60-61 6.711479872408052 1101 62-63 6.244342432941107 1101 64-65 6.741438822835168 1101 66-67 5.837802719861312 1101 68-69 6.857711536670216 1101 70-71 6.277801269378372 1101 72-73 5.559600192824096 1101 74-75 5.013701193269068 1101 76-77 5.071175506758898 1101 78-79 5.1678712965025255 1101 80-81 4.9981117925724625 1101 82-83 4.5696346795250875 1101 84-85 4.2281226734364346 1101 86-87 4.057759895370371 1101 88-89 4.046421677114488 1101 90-91 3.858697470010725 1101 92-93 3.8705881284568022 1101 94-95 3.6450536311877144 1101 96-97 3.695365137494189 1101 98-99 3.520264106096011 1101 100 3.689285765773061 1102 1 6.629333267957286 1102 2 10.457170427861563 1102 3 8.117956719486369 1102 4 12.490562005656109 1102 5 10.292413872194796 1102 6 9.344148100932674 1102 7 6.631312009584953 1102 8 7.644763830814014 1102 9 6.388911702382018 1102 10-11 7.40065994696241 1102 12-13 8.930007963665965 1102 14-15 6.044108336268419 1102 16-17 7.192141441943754 1102 18-19 7.257553071808033 1102 20-21 6.782306936846677 1102 22-23 6.326192304233167 1102 24-25 6.839665383606523 1102 26-27 8.384720928780087 1102 28-29 7.479917858622482 1102 30-31 4.041372580361877 1102 32-33 4.3892722248156915 1102 34-35 4.979427725207152 1102 36-37 5.069788447538727 1102 38-39 4.421491625393546 1102 40-41 6.211179498468379 1102 42-43 6.843438162842208 1102 44-45 6.465245650522792 1102 46-47 5.384720591638246 1102 48-49 3.5937447415810944 1102 50-51 4.978776704584664 1102 52-53 5.823192413008947 1102 54-55 5.433952614954261 1102 56-57 6.268555229045663 1102 58-59 6.03110470168469 1102 60-61 5.99898659242724 1102 62-63 4.308040445933013 1102 64-65 6.0794528232390945 1102 66-67 3.8818030629098565 1102 68-69 6.044277612822228 1102 70-71 5.344604017260721 1102 72-73 5.087627285188592 1102 74-75 4.145809041272095 1102 76-77 3.3442477976217084 1102 78-79 4.329794311221583 1102 80-81 4.403506098548998 1102 82-83 3.89068696769327 1102 84-85 3.067916423441396 1102 86-87 3.282778430844264 1102 88-89 3.513632178952683 1102 90-91 3.3728138381113375 1102 92-93 3.160002870703206 1102 94-95 3.1020066639777006 1102 96-97 3.2033084185470315 1102 98-99 3.1193933027827363 1102 100 3.201095555944349 1103 1 6.4291715147285124 1103 2 10.816366929227822 1103 3 7.787081880303578 1103 4 11.73385311176223 1103 5 10.0844856078071 1103 6 8.83813904029886 1103 7 7.7599641660687375 1103 8 7.830924803599196 1103 9 6.964849461198483 1103 10-11 7.306923240808789 1103 12-13 8.459349963860923 1103 14-15 8.21987170564159 1103 16-17 7.785555464688294 1103 18-19 6.940806585579409 1103 20-21 8.099005694334 1103 22-23 6.545528020211087 1103 24-25 5.003945330789399 1103 26-27 5.615846571806866 1103 28-29 6.732641866590885 1103 30-31 5.817710574731581 1103 32-33 7.760211287599116 1103 34-35 6.949049531214314 1103 36-37 3.7995920891569632 1103 38-39 6.220512149317145 1103 40-41 6.437184932847533 1103 42-43 7.248207543051219 1103 44-45 5.417548580446191 1103 46-47 6.056052006461769 1103 48-49 5.336185243246877 1103 50-51 6.215615958057125 1103 52-53 5.840320777582619 1103 54-55 4.590990663677442 1103 56-57 5.656475839082727 1103 58-59 5.2341905362784225 1103 60-61 4.80470709388646 1103 62-63 2.540095689913624 1103 64-65 4.762030912785477 1103 66-67 3.7892859209084975 1103 68-69 5.005599015503364 1103 70-71 3.4501681717898443 1103 72-73 4.188942653794879 1103 74-75 3.0805036160594383 1103 76-77 3.393072040626926 1103 78-79 2.822098767819174 1103 80-81 3.927235271388529 1103 82-83 3.2560771911041257 1103 84-85 3.042947957493979 1103 86-87 2.572320180057851 1103 88-89 2.848710980193907 1103 90-91 2.528488289202574 1103 92-93 2.201032279999712 1103 94-95 2.6701706494429907 1103 96-97 2.7962306294940262 1103 98-99 2.586081990030131 1103 100 2.6910080481415264 1104 1 6.296610959341873 1104 2 9.640323045575244 1104 3 7.340639192556544 1104 4 12.19689912372128 1104 5 9.40515805130563 1104 6 8.232230456129404 1104 7 3.7268892699503624 1104 8 6.444632110784763 1104 9 6.550490993579146 1104 10-11 7.220244159021011 1104 12-13 7.42008777677264 1104 14-15 7.255275070392781 1104 16-17 7.157795000514788 1104 18-19 6.870977863576769 1104 20-21 7.565042737001473 1104 22-23 6.572793146389252 1104 24-25 5.9847867719569265 1104 26-27 8.198987344078066 1104 28-29 7.166962075367611 1104 30-31 6.354693700301663 1104 32-33 7.654320439316965 1104 34-35 8.042762159166074 1104 36-37 6.54833157430949 1104 38-39 7.245712311473831 1104 40-41 7.102580949873985 1104 42-43 7.307593627980783 1104 44-45 6.740581163804858 1104 46-47 6.835703876331031 1104 48-49 5.579550754547174 1104 50-51 5.77419632696358 1104 52-53 5.976740155306338 1104 54-55 4.749635641887636 1104 56-57 6.0084559242149815 1104 58-59 3.8283376934690594 1104 60-61 4.238862612281107 1104 62-63 3.8118132839138283 1104 64-65 2.7326590165657514 1104 66-67 3.096614642911131 1104 68-69 4.307798265528703 1104 70-71 2.663864077333578 1104 72-73 2.5074703217790706 1104 74-75 3.2562181189478565 1104 76-77 3.8136241334616265 1104 78-79 4.406641942036259 1104 80-81 2.9734055865805793 1104 82-83 2.7959639347952177 1104 84-85 2.859933870132064 1104 86-87 0.671316835576242 1104 88-89 0.3403915822005956 1104 90-91 2.2555684554131723 1104 92-93 2.366907165983296 1104 94-95 2.3757017125827495 1104 96-97 2.7712242952485617 1104 98-99 2.5682536227452424 1104 100 0.9735103284698958 1105 1 5.766519368457324 1105 2 -2.7763788603023514 1105 3 0.48637403288920567 1105 4 8.506357549035627 1105 5 8.601145358611465 1105 6 7.982826060008147 1105 7 5.269657602791241 1105 8 5.933990371588543 1105 9 4.983221441298937 1105 10-11 4.973784493710372 1105 12-13 5.137235714031682 1105 14-15 5.850704406775485 1105 16-17 6.0175384961531435 1105 18-19 6.178632346448431 1105 20-21 7.033854856731832 1105 22-23 1.8112999924076973 1105 24-25 3.957913991081597 1105 26-27 6.927225011679031 1105 28-29 5.950711408636625 1105 30-31 5.388684125688361 1105 32-33 7.117822104979542 1105 34-35 6.2324124796629015 1105 36-37 6.115504481432016 1105 38-39 4.079885323848025 1105 40-41 5.409660975134383 1105 42-43 5.431290083246903 1105 44-45 6.063937072343347 1105 46-47 5.441662834207882 1105 48-49 4.984465555509129 1105 50-51 5.924021729784215 1105 52-53 5.553506875932769 1105 54-55 5.241607967823565 1105 56-57 5.173712168188366 1105 58-59 1.7461691499055547 1105 60-61 4.317098594210709 1105 62-63 -0.9762367451674301 1105 64-65 -0.7224037945639559 1105 66-67 0.688129219994007 1105 68-69 2.8649086191688475 1105 70-71 2.849147412649515 1105 72-73 2.559382517213887 1105 74-75 2.6164253660738552 1105 76-77 1.5781153978467088 1105 78-79 3.0520352763161256 1105 80-81 1.3805842304675693 1105 82-83 0.5771584481131171 1105 84-85 1.2564924281621899 1105 86-87 -0.48430364497439626 1105 88-89 1.5003562022886427 1105 90-91 1.330093795027988 1105 92-93 1.5793486951147138 1105 94-95 0.8924431974755684 1105 96-97 1.928709978005763 1105 98-99 1.854983551881265 1105 100 1.9596355264334946 1106 1 5.969921273909943 1106 2 10.316606223631013 1106 3 6.6171101752824235 1106 4 11.627366460587524 1106 5 9.745863009815924 1106 6 9.26778776927095 1106 7 6.533290837984957 1106 8 7.273920272166997 1106 9 6.845392138869961 1106 10-11 5.693116050007955 1106 12-13 7.825455319499092 1106 14-15 7.75569423021599 1106 16-17 7.647787227133691 1106 18-19 7.100154677994695 1106 20-21 8.126614757390922 1106 22-23 6.067028979313967 1106 24-25 5.013198194781616 1106 26-27 7.80470183808065 1106 28-29 6.178384166974585 1106 30-31 5.2552863018239755 1106 32-33 7.249552218028381 1106 34-35 6.742504969670968 1106 36-37 5.836147457854853 1106 38-39 7.004991320195323 1106 40-41 6.628568112255923 1106 42-43 5.990448123210594 1106 44-45 4.246229951966516 1106 46-47 5.894391831147132 1106 48-49 5.784062261486202 1106 50-51 6.451604079492579 1106 52-53 5.559340936391315 1106 54-55 5.239107000385761 1106 56-57 5.090633418498303 1106 58-59 5.03289885990521 1106 60-61 4.837634781893403 1106 62-63 4.894141385758967 1106 64-65 5.113450226210283 1106 66-67 2.863572152952994 1106 68-69 3.253519729237631 1106 70-71 2.7892681954064287 1106 72-73 2.6658318923332978 1106 74-75 2.7793956099730366 1106 76-77 3.47333646134355 1106 78-79 4.149835074852746 1106 80-81 3.6507530499278538 1106 82-83 1.9057362667715338 1106 84-85 2.63630799104493 1106 86-87 3.238355921320192 1106 88-89 3.2687568480295965 1106 90-91 3.055081173294557 1106 92-93 2.843640714136715 1106 94-95 1.4539430876050794 1106 96-97 1.3595151750063295 1106 98-99 1.9194486545148308 1106 100 2.7812319051277044 1107 1 6.010320962940188 1107 2 10.277845395582634 1107 3 5.993590927698147 1107 4 11.211505080190022 1107 5 9.157460973752148 1107 6 7.902700691916753 1107 7 3.462185824213808 1107 8 6.142489996815762 1107 9 5.671428313140318 1107 10-11 4.461885256256441 1107 12-13 5.9943870116422815 1107 14-15 6.090711233750763 1107 16-17 6.815006486675223 1107 18-19 6.129526654235647 1107 20-21 5.438632768828445 1107 22-23 5.541797556418793 1107 24-25 4.486375888863158 1107 26-27 6.962244838478359 1107 28-29 6.386528408515918 1107 30-31 5.003798165840113 1107 32-33 4.987050682747249 1107 34-35 6.6236151414450575 1107 36-37 6.421550756403821 1107 38-39 6.3025445797241275 1107 40-41 7.6120267394254775 1107 42-43 6.149685979187613 1107 44-45 3.8739263288113754 1107 46-47 3.424251955694821 1107 48-49 3.9222444290418075 1107 50-51 4.628214167917999 1107 52-53 3.786040338569432 1107 54-55 5.20639692009885 1107 56-57 5.348784193871136 1107 58-59 3.0266452993055815 1107 60-61 2.6192350519674683 1107 62-63 0.25553443113517105 1107 64-65 1.8289388735257042 1107 66-67 3.9393712879414124 1107 68-69 4.7894799065840985 1107 70-71 3.9376820722624615 1107 72-73 2.0544016413569786 1107 74-75 2.2980227443184944 1107 76-77 2.938051401446934 1107 78-79 3.800377315159345 1107 80-81 1.8602402816023513 1107 82-83 1.5511904195191093 1107 84-85 2.193379174329266 1107 86-87 2.1905282882639625 1107 88-89 2.5429249084674694 1107 90-91 1.2891662696452109 1107 92-93 2.1306670299725177 1107 94-95 2.134321632750332 1107 96-97 2.269252682896017 1107 98-99 1.6614967378272105 1107 100 -0.02788994656420485 1108 1 4.449691594288691 1108 2 -3.1249297278807617 1108 3 0.13068250855877395 1108 4 6.649643736761558 1108 5 6.856153255721669 1108 6 6.825857354596195 1108 7 4.225032168258693 1108 8 5.104023304427695 1108 9 4.810921727861899 1108 10-11 3.341108707244654 1108 12-13 2.3883454011650542 1108 14-15 3.9055858852296232 1108 16-17 4.552666148703597 1108 18-19 4.05392448048412 1108 20-21 4.675638443279691 1108 22-23 4.144140468334818 1108 24-25 2.9521446206352806 1108 26-27 4.798077054112657 1108 28-29 6.264051486390461 1108 30-31 4.596791948722359 1108 32-33 4.458126584740864 1108 34-35 2.9456676063351424 1108 36-37 1.0371483452323105 1108 38-39 2.4661171901949928 1108 40-41 5.372896640610101 1108 42-43 3.0405795885383675 1108 44-45 3.798025918536542 1108 46-47 4.843446530328862 1108 48-49 4.942080798172501 1108 50-51 2.107672048132688 1108 52-53 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-17.94049544927718 2213 58-59 -18.42303711394141 2213 60-61 -19.80494483576669 2213 62-63 -17.931679820441516 2213 64-65 -18.024345417690757 2213 66-67 -8.807264204182172 2213 68-69 -23.213439192665277 2213 70-71 -17.864105747175085 2213 72-73 -9.733997680175008 2213 74-75 -4.35279544687079 2213 76-77 -2.9866073497595984 2213 78-79 -4.864311611005714 2213 80-81 -3.3987399547369215 2213 82-83 -3.2803154233649394 2213 84-85 -2.9402838969567355 2213 86-87 -3.2572474799323814 2213 88-89 -2.885024974862276 2213 90-91 -2.8868422018338293 2213 92-93 -3.8207376049760846 2213 94-95 -3.837218508378509 2213 96-97 -3.5734675615024045 2213 98-99 -4.000113218034887 2213 100 -3.6650842376869583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 755.0 3 668.0 4 3470.0 5 14295.0 6 21570.0 7 23821.0 8 22229.0 9 22014.0 10 25818.0 11 31280.0 12 38660.0 13 50710.0 14 38732.0 15 42822.0 16 49220.0 17 52480.0 18 52897.0 19 53452.0 20 52613.0 21 51168.0 22 53611.0 23 59246.0 24 65733.0 25 72792.0 26 80831.0 27 87394.0 28 95115.0 29 101423.0 30 109125.0 31 120984.0 32 137250.0 33 166462.0 34 200071.0 35 232346.0 36 250212.0 37 208971.0 38 62593.0 39 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.384359808068695 20.55144737672833 13.72273344276785 24.34145937243513 2 14.994197897335379 21.032489360397257 40.3485414581121 23.624771284155262 3 18.12704972329462 26.308781977232915 29.800972013359956 25.76319628611251 4 12.160932879122525 17.487084939916894 38.94274323363157 31.409238947329015 5 12.978514831285112 37.6572242283303 35.9476414085035 13.416619531881086 6 30.584018584280017 37.881487291086216 18.807465664832606 12.727028459801165 7 26.020026256277273 31.776554563008926 24.642463287376263 17.56095589333754 8 23.735999929885114 36.95759563311749 21.88042543799892 17.425978998998474 9 24.594867073983202 17.055886789466122 22.06463530928726 36.284610827263414 10-11 23.141348429067886 27.26735436037237 29.489024312330713 20.102272898229028 12-13 24.052512923969235 24.904703063926366 29.264304627411423 21.77847938469298 14-15 21.504817654992422 26.504080584301747 26.46967086603941 25.521430894666413 16-17 21.126371727552247 29.9539877603493 27.383878338108996 21.535762173989458 18-19 21.13918142712699 29.14307835255195 28.82267272265372 20.89506749766735 20-21 22.360564782055583 28.598044924334964 27.876422477357455 21.164967816252002 22-23 21.506433518760275 28.54641586494055 28.23306829415716 21.714082322142016 24-25 21.79117626587718 28.445585075844765 27.73791592338269 22.02532273489536 26-27 21.698038102029336 28.339621325736818 27.942005488985046 22.020335083248803 28-29 21.592126954355447 28.248143704393197 28.36047826419427 21.799251077057082 30-31 22.10053779458618 28.700548890969674 27.704192456891306 21.49472085755284 32-33 21.222955224839062 28.595471568820823 28.26896088353329 21.912612322806826 34-35 21.335542709372902 28.087536326033607 28.211342980523597 22.365577984069894 36-37 21.989675951534437 28.60379069662895 27.728572517547168 21.677960834289443 38-39 21.752694199639617 28.33412477073684 27.918460165943966 21.994720863679575 40-41 21.81922577183676 28.37436495070777 27.655319356338094 22.15108992111738 42-43 21.689666626991443 28.405631703459598 28.1270882975984 21.77761337195055 44-45 21.985852689594683 28.03601677864482 28.061424240857296 21.916706290903196 46-47 21.822549246329487 28.41214860097808 28.215426249807606 21.549875902884825 48-49 21.79925716742522 28.20176560052578 28.219758740404448 21.779218491644553 50-51 21.938308572992593 28.233903186627085 27.99600641337665 21.831781827003674 52-53 21.45722863848568 28.266417669891013 28.056131596343597 22.220222095279706 54-55 21.839115226400473 28.379600985055703 27.807512121759746 21.97377166678408 56-57 22.003661694669084 28.180582288013074 27.77975031112908 22.036005706188764 58-59 21.698880119910044 27.76691467694202 28.234230014851224 22.299975188296713 60-61 22.08038100412327 28.261549178001736 27.773684522029978 21.884385295845014 62-63 21.582147519619006 28.323310283510196 28.105408917529388 21.989133279341402 64-65 21.589879242608973 27.896903274966977 28.154374446448415 22.358843035975635 66-67 21.789321735511642 28.01507130117133 27.896897920797358 22.298709042519672 68-69 22.210954317881797 27.977180961820313 27.80645178585858 22.00541293443931 70-71 22.161658350723922 28.055783001204738 27.68923954012849 22.09331910794285 72-73 21.92402829464233 28.150880146494185 27.98380368007561 21.94128787878788 74-75 22.11192165791733 27.788853635863646 27.696022840735917 22.4032018654831 76-77 22.03142824082463 28.105006329173303 27.87490808855655 21.988657341445517 78-79 22.070676459139076 27.942219084368308 27.989194385609682 21.997910070882938 80-81 22.082803711795744 27.86903818252461 27.85100442987712 22.197153675802532 82-83 21.60005553980218 28.06886715655996 28.120230482851905 22.210846820785953 84-85 21.870384991504892 27.79131901955605 27.7099182718097 22.628377717129368 86-87 22.01694388040542 27.969165447313436 27.77093340751433 22.242957264766808 88-89 21.821937168277895 27.551461623846613 28.150747637826353 22.475853570049143 90-91 22.111815358428323 27.611638521398007 27.82632360100386 22.45022251916981 92-93 21.722868563634503 27.90014603979844 28.045730090524255 22.331255306042795 94-95 21.64052345949022 27.573755876080963 28.06416814635886 22.72155251806996 96-97 21.853412729080272 27.674146297279705 27.98062756435869 22.491813409281324 98-99 21.845165568690554 27.532043742886543 27.960589284792093 22.662201403630814 100 21.93389076900264 27.389122188564414 27.811764293389295 22.86522274904365 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 367.0 1 401.5 2 370.0 3 315.5 4 349.5 5 429.0 6 622.0 7 961.5 8 1385.0 9 1970.5 10 2750.5 11 3703.0 12 4752.5 13 5710.0 14 6729.0 15 7695.0 16 8529.5 17 9328.5 18 10088.5 19 10828.5 20 11598.0 21 12454.5 22 13510.5 23 14944.0 24 16572.5 25 18971.5 26 22629.5 27 27452.0 28 33132.5 29 39923.5 30 47661.5 31 55768.0 32 64246.0 33 72658.5 34 80129.0 35 86501.5 36 92235.5 37 97313.0 38 101474.5 39 104549.5 40 106092.5 41 105596.5 42 103818.5 43 101045.5 44 97568.5 45 93584.0 46 89670.5 47 86330.0 48 83167.5 49 81611.5 50 81328.0 51 77482.5 52 69753.5 53 62547.0 54 57155.5 55 52429.0 56 47499.5 57 42816.0 58 38421.5 59 33797.5 60 29104.0 61 24633.5 62 20399.5 63 16627.0 64 13391.5 65 10776.5 66 8565.0 67 6626.5 68 5116.5 69 3931.0 70 2970.0 71 2216.0 72 1681.5 73 1264.5 74 918.5 75 674.5 76 478.0 77 311.5 78 225.0 79 150.0 80 95.5 81 69.0 82 48.5 83 31.0 84 22.0 85 18.0 86 11.5 87 6.5 88 4.0 89 2.0 90 1.0 91 3.0 92 3.0 93 1.0 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.943766014778457 2 24.778181469461945 3 6.229008698381286 4 22.372050683527274 5 12.401423067674177 6 9.962926718698577 7 8.330736096793013 8 8.818253741655242 9 5.456012984003202 10-11 7.03950996140107 12-13 9.970791078292697 14-15 7.471341401839352 16-17 8.027495108840558 18-19 6.916023169529439 20-21 8.17101513080991 22-23 6.007571580202301 24-25 5.5365454792464455 26-27 9.885463684821168 28-29 6.41837085341197 30-31 4.4112337200491405 32-33 8.92906311321118 34-35 7.402669014713799 36-37 7.076870210096457 38-39 6.330882123239055 40-41 8.329428397276208 42-43 6.613036456483029 44-45 6.569264847656639 46-47 6.068016357867956 48-49 4.105849557888589 50-51 6.5676302232606325 52-53 4.552065693011727 54-55 5.125237656224686 56-57 5.21185458671973 58-59 3.5938670345661303 60-61 4.255617477549342 62-63 3.253447422851177 64-65 4.705012048998048 66-67 2.588990768367911 68-69 5.149902322111092 70-71 2.489133380265216 72-73 1.6086883555896527 74-75 0.8633359559973642 76-77 1.2697943933109717 78-79 1.8323049729633125 80-81 2.2068155845816415 82-83 0.8482610865675285 84-85 1.267451431676696 86-87 0.5622199797597175 88-89 0.8267748570066904 90-91 0.4250568304415011 92-93 0.3698065258564887 94-95 0.5458010858264988 96-97 0.5416781998499052 98-99 0.4707899885430992 100 0.5416781998499052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2752926.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.83504222889997 #Duplication Level Percentage of deduplicated Percentage of total 1 96.67002223300071 91.6770564073532 2 2.6281090941864855 4.984736738586628 3 0.42782476633934857 1.2171833934708416 4 0.1328779308816708 0.5040593674580841 5 0.04951479083566742 0.23478686399278342 6 0.029602365281781527 0.16844049369438444 7 0.015667103204811395 0.10400532758229758 8 0.008314174474396946 0.06307800699023014 9 0.007791446857593153 0.0665011972587669 >10 0.028386869807268116 0.4053321502580463 >50 4.611279946042505E-4 0.03129572426012783 >100 0.0010817585567348862 0.20719118365788852 >500 2.693744504222096E-4 0.16422516880984422 >1k 7.696412853184081E-5 0.172107976626903 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.632498657791746E-5 0.0 10-11 0.0 1.816249328895873E-5 0.0 3.632498657791746E-5 0.0 12-13 0.0 3.632498657791746E-5 0.0 5.4487479866876184E-5 0.0 14-15 0.0 3.632498657791746E-5 0.0 1.0897495973375237E-4 0.0 16-17 0.0 3.632498657791746E-5 0.0 1.4529994631166983E-4 0.0 18-19 0.0 3.632498657791746E-5 0.0 1.99787426178546E-4 0.0 20-21 0.0 3.632498657791746E-5 0.0 2.9059989262333966E-4 0.0 22-23 1.816249328895873E-5 3.632498657791746E-5 0.0 2.9059989262333966E-4 0.0 24-25 3.632498657791746E-5 3.632498657791746E-5 0.0 2.9059989262333966E-4 0.0 26-27 3.632498657791746E-5 3.632498657791746E-5 0.0 5.267123053798032E-4 0.0 28-29 3.632498657791746E-5 3.632498657791746E-5 0.0 6.538497584025143E-4 0.0 30-31 3.632498657791746E-5 3.632498657791746E-5 0.0 0.0021250117148081714 0.0 32-33 7.264997315583492E-5 3.632498657791746E-5 0.0 0.006138922731668051 0.0 34-35 7.264997315583492E-5 3.632498657791746E-5 0.0 0.013240457607650913 0.0 36-37 7.264997315583492E-5 3.632498657791746E-5 0.0 0.023247991409867177 0.0 38-39 7.264997315583492E-5 3.632498657791746E-5 0.0 0.038250210866547084 0.0 40-41 7.264997315583492E-5 3.632498657791746E-5 0.0 0.058701178309914614 0.0 42-43 7.264997315583492E-5 3.632498657791746E-5 0.0 0.07913398325999318 0.0 44-45 7.264997315583492E-5 3.632498657791746E-5 0.0 0.10176444989803576 0.0 46-47 7.264997315583492E-5 3.632498657791746E-5 0.0 0.1302250768818341 0.0 48-49 7.264997315583492E-5 3.632498657791746E-5 0.0 0.16017502831532704 0.0 50-51 7.264997315583492E-5 3.632498657791746E-5 0.0 0.1942660282187026 0.0 52-53 7.264997315583492E-5 3.632498657791746E-5 0.0 0.2299371650382175 0.0 54-55 7.264997315583492E-5 3.632498657791746E-5 0.0 0.2700217877269494 0.0 56-57 1.0897495973375238E-4 5.4487479866876184E-5 0.0 0.316862857919174 0.0 58-59 1.0897495973375238E-4 9.081246644479365E-5 0.0 0.37071465052093666 0.0 60-61 1.0897495973375238E-4 1.0897495973375238E-4 0.0 0.4300515160959648 0.0 62-63 1.0897495973375238E-4 1.0897495973375238E-4 0.0 0.49391084249994366 0.0 64-65 1.0897495973375238E-4 1.0897495973375238E-4 0.0 0.5650170037262171 0.0 66-67 1.0897495973375238E-4 1.0897495973375238E-4 0.0 0.6495270849997421 0.0 68-69 1.0897495973375238E-4 1.0897495973375238E-4 0.0 0.744208162515084 0.0 70-71 1.0897495973375238E-4 1.0897495973375238E-4 0.0 0.8592857199939264 0.0 72-73 1.0897495973375238E-4 1.0897495973375238E-4 0.0 0.9791400132077652 0.0 74-75 1.0897495973375238E-4 1.0897495973375238E-4 0.0 1.1057689164183855 0.0 76-77 1.0897495973375238E-4 1.0897495973375238E-4 0.0 1.2477996139380427 0.0 78-79 1.0897495973375238E-4 1.0897495973375238E-4 0.0 1.4063218553640744 0.0 80-81 1.0897495973375238E-4 1.0897495973375238E-4 0.0 1.5845504020086265 0.0 82-83 1.0897495973375238E-4 1.0897495973375238E-4 0.0 1.7815771292072506 0.0 84-85 1.0897495973375238E-4 1.0897495973375238E-4 0.0 1.98252695495629 3.632498657791746E-5 86-87 1.0897495973375238E-4 1.0897495973375238E-4 0.0 2.2043454854943434 3.632498657791746E-5 88 1.0897495973375238E-4 1.0897495973375238E-4 0.0 2.3897845419746115 3.632498657791746E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACGCG 145 4.481894E-4 20.599167 5 CCGTCCG 125 0.004566952 19.937386 9 TCTATAC 890 0.0 19.147516 3 GTCGTAC 165 0.0072418875 18.407986 1 GTATATA 1640 0.0 18.149822 1 AGTACCG 225 2.366504E-5 17.700027 5 GTATTAT 1340 0.0 17.679907 1 CTATACC 790 0.0 16.70072 4 GTATTAG 1000 0.0 16.401514 1 CTTACAC 1165 0.0 16.0433 3 GTACATG 10215 0.0 15.877899 1 TACATGG 10285 0.0 15.7657 2 GTGTATA 1525 0.0 15.5351305 1 GTATTAA 1275 0.0 15.246141 1 CATGGGG 3600 0.0 14.659521 4 ACATGGG 10520 0.0 14.265521 3 CTACGCT 235 0.005837992 14.03571 4 TACACTG 1635 0.0 14.0057535 5 TAAGCGC 250 0.0010514309 13.941168 6 CTCTATA 965 0.0 13.845277 2 >>END_MODULE