FastQCFastQC Report
Wed 25 May 2016
SRR1295116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752926
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68540.24897145800504625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51700.18780018060783327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG2953.417881E-919.1313925
CCGTACA1951.5909212E-417.8696982
CATGGGG42450.016.6188474
TAGAGCG1800.002798940815.6771135
ACCGTCC2406.796937E-515.6497628
GAGTACT29350.015.43507712-13
TACCGTT2206.23218E-414.9384117
GTACATG132000.014.7948011
GTATAGA10150.014.72489551
GTACCGT2257.3816255E-414.608576
GTATTAG10950.014.5590441
ACACCGT3606.954524E-714.3477016
GTATATA18850.014.2718211
TACATGG134300.014.1592782
CTATGCG3002.868728E-514.0845319
GTACGGA3203.0122703E-514.0116581
TATACTG15800.013.9903355
GTATAAG10050.013.8799841
ACATGGG132300.013.8636093
GTATAGG7250.013.7432361
Produced by FastQC (version 0.11.5)