##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295116_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2752926 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.57260057117409 33.0 33.0 33.0 27.0 33.0 2 30.18101031411669 33.0 33.0 33.0 27.0 33.0 3 31.023035853488253 33.0 33.0 33.0 27.0 33.0 4 35.36004781821233 37.0 37.0 37.0 33.0 37.0 5 35.651077072177024 37.0 37.0 37.0 33.0 37.0 6 35.46233280516803 37.0 37.0 37.0 33.0 37.0 7 35.378377769689415 37.0 37.0 37.0 33.0 37.0 8 35.363821257818046 37.0 37.0 37.0 33.0 37.0 9 35.344244269551744 37.0 37.0 37.0 33.0 37.0 10-11 35.318632792890185 37.0 37.0 37.0 33.0 37.0 12-13 35.244177649526364 37.0 37.0 37.0 33.0 37.0 14-15 36.768223156016546 40.0 37.0 40.0 33.0 40.0 16-17 36.75750710335113 40.0 37.0 40.0 33.0 40.0 18-19 36.77699364240085 40.0 37.0 40.0 33.0 40.0 20-21 36.77414558182821 40.0 37.0 40.0 33.0 40.0 22-23 36.73602614091334 40.0 37.0 40.0 33.0 40.0 24-25 36.70585896606011 40.0 37.0 40.0 33.0 40.0 26-27 36.68345135321472 40.0 37.0 40.0 33.0 40.0 28-29 36.634696319479715 40.0 37.0 40.0 33.0 40.0 30-31 36.557284322208446 40.0 37.0 40.0 33.0 40.0 32-33 36.50898825467884 40.0 37.0 40.0 33.0 40.0 34-35 36.42207818154211 40.0 37.0 40.0 33.0 40.0 36-37 36.424699574198506 38.5 37.0 40.0 33.0 40.0 38-39 36.36790436793434 37.0 37.0 40.0 33.0 40.0 40-41 36.30970883343758 37.0 37.0 40.0 33.0 40.0 42-43 36.2191642637688 37.0 37.0 40.0 30.0 40.0 44-45 36.11533219563475 37.0 37.0 40.0 27.0 40.0 46-47 36.02433955725653 37.0 37.0 40.0 27.0 40.0 48-49 35.92968499698139 37.0 37.0 40.0 27.0 40.0 50-51 35.834771621176884 37.0 37.0 40.0 27.0 40.0 52-53 35.73891452222108 37.0 37.0 40.0 27.0 40.0 54-55 35.619041158389294 37.0 37.0 40.0 27.0 40.0 56-57 35.49342663042886 37.0 35.0 40.0 27.0 40.0 58-59 35.3593523763443 37.0 33.0 40.0 27.0 40.0 60-61 35.1982790311109 37.0 33.0 40.0 27.0 40.0 62-63 35.01510501916869 37.0 33.0 40.0 27.0 40.0 64-65 34.82452234458899 37.0 33.0 40.0 27.0 40.0 66-67 34.59594754817238 37.0 33.0 40.0 27.0 40.0 68-69 34.37889521912322 37.0 33.0 37.0 27.0 40.0 70-71 34.14476742200844 37.0 33.0 37.0 27.0 40.0 72-73 33.924862855013174 37.0 33.0 37.0 27.0 40.0 74-75 33.68228568439544 37.0 33.0 37.0 27.0 40.0 76-77 33.44473770816941 37.0 33.0 37.0 27.0 37.0 78-79 33.20921830081883 37.0 33.0 37.0 27.0 37.0 80-81 32.99658581451155 37.0 33.0 37.0 27.0 37.0 82-83 32.792001310605514 37.0 33.0 37.0 27.0 37.0 84-85 32.6174259678611 37.0 33.0 37.0 22.0 37.0 86-87 32.645099069135895 37.0 33.0 37.0 24.5 37.0 88-89 32.66216963332832 37.0 33.0 37.0 27.0 37.0 90-91 32.680882268539 37.0 33.0 37.0 27.0 37.0 92-93 32.65329108010894 37.0 33.0 37.0 27.0 37.0 94-95 32.69246866788283 37.0 33.0 37.0 27.0 37.0 96-97 32.65776268595668 37.0 33.0 37.0 27.0 37.0 98-99 32.57940533090973 33.0 33.0 37.0 22.0 37.0 100 32.051299962294664 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5834948884426439 1101 2 4.083351050587993 1101 3 2.304397378365362 1101 4 1.0907473601704751 1101 5 0.4291506784830119 1101 6 0.46414631031040443 1101 7 0.528954898403633 1101 8 0.487844544758147 1101 9 0.595718718955915 1101 10-11 0.6200308896352311 1101 12-13 0.5767566386307408 1101 14-15 0.8368059945148403 1101 16-17 0.8776916427088466 1101 18-19 0.9193450500200981 1101 20-21 0.858653834627944 1101 22-23 0.7919549688186365 1101 24-25 0.8333169908330404 1101 26-27 0.8461725170605163 1101 28-29 0.7134963636854792 1101 30-31 0.6146319969170619 1101 32-33 0.8052918225350751 1101 34-35 1.015775159954714 1101 36-37 0.7932603224514096 1101 38-39 0.8110186906450139 1101 40-41 0.8180003268450591 1101 42-43 0.9041977358777231 1101 44-45 0.8493895935549531 1101 46-47 0.8874385834231617 1101 48-49 0.823884146866618 1101 50-51 0.8049989279907521 1101 52-53 0.7882797014531207 1101 54-55 0.8439361595507151 1101 56-57 0.8577878804584813 1101 58-59 0.871816520397239 1101 60-61 0.8557997249069658 1101 62-63 0.812883991153889 1101 64-65 0.7604193539483717 1101 66-67 0.7437743052357817 1101 68-69 0.7481690781266792 1101 70-71 0.7445744702554578 1101 72-73 0.7171969159537781 1101 74-75 0.6502553779240259 1101 76-77 0.7742851647003732 1101 78-79 0.8146736632441858 1101 80-81 0.7670914241688038 1101 82-83 0.7628358468058849 1101 84-85 0.7044597350706994 1101 86-87 0.7277478926996181 1101 88-89 0.731055048951518 1101 90-91 0.6919377173647234 1101 92-93 0.7565871034716238 1101 94-95 0.7771958148364675 1101 96-97 0.7433873996498903 1101 98-99 0.7022023004277074 1101 100 0.7509667935776605 1102 1 0.5165130988674562 1102 2 3.9877565773752934 1102 3 2.235257135833219 1102 4 1.0677979420956802 1102 5 0.4104423568294706 1102 6 0.4299347356514218 1102 7 0.5349968408524788 1102 8 0.4957437403675442 1102 9 0.5342674224521247 1102 10-11 0.5883628505096965 1102 12-13 0.5937757903214589 1102 14-15 0.846419149050007 1102 16-17 0.8526244028123031 1102 18-19 0.8809802321892022 1102 20-21 0.8519229628267198 1102 22-23 0.8987423271622177 1102 24-25 0.8464761185050662 1102 26-27 0.8181933879644205 1102 28-29 0.7987772783545424 1102 30-31 0.7814044765889179 1102 32-33 0.9161896356270844 1102 34-35 1.0839924536775882 1102 36-37 0.9071886491289192 1102 38-39 0.8335293586341095 1102 40-41 0.8966780864733863 1102 42-43 0.9674964123085701 1102 44-45 1.0468865171030401 1102 46-47 0.9993415075678769 1102 48-49 0.8772421712246512 1102 50-51 0.8581541275452054 1102 52-53 0.9436193473031409 1102 54-55 0.8551829768887487 1102 56-57 0.837946333944835 1102 58-59 0.8545395793737995 1102 60-61 0.8240223522935111 1102 62-63 0.9004673279944697 1102 64-65 0.8796595728959247 1102 66-67 0.8856354521031662 1102 68-69 0.8643999328548801 1102 70-71 0.8567003627176746 1102 72-73 0.8255494597407775 1102 74-75 0.7701739780106891 1102 76-77 0.8608082406705364 1102 78-79 0.7628798052424628 1102 80-81 0.757561583313155 1102 82-83 0.8103983625057793 1102 84-85 0.8512130426700963 1102 86-87 0.8502377421950129 1102 88-89 0.7284483564873909 1102 90-91 0.8287490494015657 1102 92-93 0.8729123494885229 1102 94-95 0.7587766624575423 1102 96-97 0.7534899806931179 1102 98-99 0.8967725778655691 1102 100 0.8208497256156662 1103 1 0.3802224878814684 1103 2 3.954041607769991 1103 3 2.256094463372289 1103 4 0.9301185578779396 1103 5 0.2617166133585087 1103 6 0.3437548650281812 1103 7 0.33576779518200084 1103 8 0.37791176619384004 1103 9 0.38417533785436575 1103 10-11 0.4715991851618355 1103 12-13 0.4684636370911406 1103 14-15 0.6015717551441782 1103 16-17 0.6193162692860881 1103 18-19 0.6651210486790902 1103 20-21 0.6286781297958157 1103 22-23 0.6150676223784046 1103 24-25 0.5384955824713558 1103 26-27 0.622617628452069 1103 28-29 0.5528134030809113 1103 30-31 0.6019805992108687 1103 32-33 0.5942715002223835 1103 34-35 0.7782427824128249 1103 36-37 0.6613508535819008 1103 38-39 0.5598627616844922 1103 40-41 0.5731947469107013 1103 42-43 0.6597117940836341 1103 44-45 0.668593960172295 1103 46-47 0.6187279943387409 1103 48-49 0.5753705509682305 1103 50-51 0.5967706815349203 1103 52-53 0.6261478925601551 1103 54-55 0.5750360030281172 1103 56-57 0.5450461032330551 1103 58-59 0.5210992497412761 1103 60-61 0.5204493214288703 1103 62-63 0.5895278520729903 1103 64-65 0.5048551679405975 1103 66-67 0.5468531345407328 1103 68-69 0.5808639359430856 1103 70-71 0.5465048177014467 1103 72-73 0.5233699225328508 1103 74-75 0.553344323542575 1103 76-77 0.5061079642553565 1103 78-79 0.652391924711651 1103 80-81 0.5238615334783745 1103 82-83 0.5954826588058069 1103 84-85 0.6186609840483968 1103 86-87 0.5446227845965339 1103 88-89 0.5249129518477176 1103 90-91 0.6437594278812071 1103 92-93 0.6180226750993967 1103 94-95 0.5267736255701223 1103 96-97 0.6264593103572764 1103 98-99 0.5510013685524058 1103 100 0.5886224887152096 1104 1 0.49009630971181295 1104 2 3.658592129711817 1104 3 2.1879785213433074 1104 4 0.9487361721477257 1104 5 0.4284010159124776 1104 6 0.401196336600087 1104 7 0.3999362354374014 1104 8 0.40186534884307434 1104 9 0.37071126569463786 1104 10-11 0.5175059957425603 1104 12-13 0.4642310942716392 1104 14-15 0.6859971123956186 1104 16-17 0.6229863311083221 1104 18-19 0.6726397980456653 1104 20-21 0.7212632620548618 1104 22-23 0.6444711898254525 1104 24-25 0.6083825795322753 1104 26-27 0.5825167872642751 1104 28-29 0.6001922923076322 1104 30-31 0.5599554142509007 1104 32-33 0.7754714596832102 1104 34-35 0.80614893423202 1104 36-37 0.6280884637977664 1104 38-39 0.6826305735827418 1104 40-41 0.683259862954074 1104 42-43 0.6594609579632262 1104 44-45 0.6904306379872409 1104 46-47 0.7311659187331898 1104 48-49 0.6212748278507689 1104 50-51 0.634384697953287 1104 52-53 0.5898184611220358 1104 54-55 0.6581438372229087 1104 56-57 0.5763157393939906 1104 58-59 0.6600041853853398 1104 60-61 0.6662357813457689 1104 62-63 0.7602433684406762 1104 64-65 0.6340281688527298 1104 66-67 0.5801028558339354 1104 68-69 0.5486885026807045 1104 70-71 0.49132213806224456 1104 72-73 0.57607971274264 1104 74-75 0.5798227364340249 1104 76-77 0.615532054657713 1104 78-79 0.5428778880237104 1104 80-81 0.5772503548019827 1104 82-83 0.5099247384652799 1104 84-85 0.5782016245672992 1104 86-87 0.583817828682875 1104 88-89 0.5575634535199612 1104 90-91 0.573470839657837 1104 92-93 0.6035514579107897 1104 94-95 0.4978273906456252 1104 96-97 0.5309008832771056 1104 98-99 0.5899139052910272 1104 100 0.46732168806658336 1105 1 0.4082465498994239 1105 2 3.61118770727764 1105 3 2.1184988209378126 1105 4 0.9872943831762413 1105 5 0.3275906876533341 1105 6 0.3672248236237081 1105 7 0.29069483688384423 1105 8 0.3287750774417617 1105 9 0.3605867946254193 1105 10-11 0.3127466465706803 1105 12-13 0.3408304400666964 1105 14-15 0.4614993054129428 1105 16-17 0.4151302803156227 1105 18-19 0.45192381530475245 1105 20-21 0.3224487275660266 1105 22-23 0.34941771704095714 1105 24-25 0.39169444682306676 1105 26-27 0.47472898560447874 1105 28-29 0.47522910298453525 1105 30-31 0.42827156287284396 1105 32-33 0.48333513025862374 1105 34-35 0.625170968012327 1105 36-37 0.46428473405117643 1105 38-39 0.47350326620015437 1105 40-41 0.3975419807493381 1105 42-43 0.5460383406939471 1105 44-45 0.5540957726573055 1105 46-47 0.5374263102803809 1105 48-49 0.36679155875567915 1105 50-51 0.4339641160979113 1105 52-53 0.39940895810180166 1105 54-55 0.3509707026032771 1105 56-57 0.35644769045854474 1105 58-59 0.3915367511854555 1105 60-61 0.3540158289173334 1105 62-63 0.38927649476077875 1105 64-65 0.3290720072716695 1105 66-67 0.39132594607792726 1105 68-69 0.3877957354990542 1105 70-71 0.45020428304752613 1105 72-73 0.42040581148516054 1105 74-75 0.32386503938452904 1105 76-77 0.34667432586825697 1105 78-79 0.3765531887995479 1105 80-81 0.25002155465463005 1105 82-83 0.3966368789718757 1105 84-85 0.35028990824687156 1105 86-87 0.39559749197619 1105 88-89 0.3765289556389746 1105 90-91 0.3872876573405577 1105 92-93 0.43715709797528035 1105 94-95 0.3890963667825531 1105 96-97 0.32824865070817566 1105 98-99 0.3985562738045516 1105 100 0.3809319904513586 1106 1 0.4245355918130542 1106 2 3.82313481434387 1106 3 2.271776709925586 1106 4 0.9464343770750148 1106 5 0.37402269905718555 1106 6 0.24025927356429122 1106 7 0.2448487570700948 1106 8 0.16986682152274568 1106 9 0.19847293377588215 1106 10-11 0.24518612884847357 1106 12-13 0.26557836267348023 1106 14-15 0.2615415477353764 1106 16-17 0.22441187634461812 1106 18-19 0.20585023159018334 1106 20-21 0.2638596935217663 1106 22-23 0.21107505582680375 1106 24-25 0.17781995562110353 1106 26-27 0.23825233700224402 1106 28-29 0.2515428324259332 1106 30-31 0.10191622316040139 1106 32-33 0.30997723500941987 1106 34-35 0.4020171907628267 1106 36-37 0.25748639737621914 1106 38-39 0.16911136602776367 1106 40-41 0.19221202584374453 1106 42-43 0.2690533726854696 1106 44-45 0.19535799230625628 1106 46-47 0.12375077022441161 1106 48-49 0.07729922535264677 1106 50-51 0.1284562527001043 1106 52-53 0.06591039988418856 1106 54-55 0.025098157001835375 1106 56-57 0.10862513408029173 1106 58-59 0.11147391915721983 1106 60-61 0.1267600515086329 1106 62-63 0.13211583829065887 1106 64-65 0.08622240454989338 1106 66-67 0.06290864185771028 1106 68-69 0.05436169974772298 1106 70-71 0.004356955098629101 1106 72-73 0.12946634654552724 1106 74-75 0.10754803551778025 1106 76-77 0.09628268687279729 1106 78-79 0.06907054406557478 1106 80-81 0.12637929083979316 1106 82-83 0.19923743907851588 1106 84-85 0.14837428159746935 1106 86-87 0.17149241751907596 1106 88-89 0.16935648446524 1106 90-91 0.06993205067621489 1106 92-93 0.13168030489274685 1106 94-95 0.14458384352938225 1106 96-97 0.04005734517505033 1106 98-99 0.10625342746294564 1106 100 0.22681713237111012 1107 1 0.39959460957708615 1107 2 3.540253547671597 1107 3 2.115127957467614 1107 4 0.9409385648886115 1107 5 0.2315873389631804 1107 6 0.17432835017884685 1107 7 0.16520174376233854 1107 8 0.1688311313653017 1107 9 0.20173223913683813 1107 10-11 0.2989367309427422 1107 12-13 0.1782772196659934 1107 14-15 0.2415235265478799 1107 16-17 0.1649800772903376 1107 18-19 0.1722140670156307 1107 20-21 0.22207797386877814 1107 22-23 0.1372367609117262 1107 24-25 0.25465155786147875 1107 26-27 0.2346315651867954 1107 28-29 0.2083471510109547 1107 30-31 0.14565210686821928 1107 32-33 0.2592012121804146 1107 34-35 0.40914564832765876 1107 36-37 0.22107091015077884 1107 38-39 0.16380112819764747 1107 40-41 0.19004861668140194 1107 42-43 0.24940247834950213 1107 44-45 0.3171868395041102 1107 46-47 0.2999517798604927 1107 48-49 0.19302671975623298 1107 50-51 0.134332327109135 1107 52-53 0.13015258850242617 1107 54-55 0.18116308960993877 1107 56-57 0.17061164093959036 1107 58-59 0.21340020101710167 1107 60-61 0.17968044398269 1107 62-63 0.25630753562570874 1107 64-65 0.12815266222931143 1107 66-67 0.1226122212192493 1107 68-69 0.13607661317411157 1107 70-71 0.14054166604124418 1107 72-73 0.21407005307641924 1107 74-75 0.21652967727938233 1107 76-77 0.13623799411116266 1107 78-79 0.07162745915461244 1107 80-81 0.16009991449637795 1107 82-83 0.2684927759324012 1107 84-85 0.29729006504584277 1107 86-87 0.2030976912216147 1107 88-89 0.20566638768193002 1107 90-91 0.20985783718364814 1107 92-93 0.29087067040949677 1107 94-95 0.18806096042646203 1107 96-97 0.2100836470629801 1107 98-99 0.19532393204130472 1107 100 0.19793610755216662 1108 1 0.39170204617881765 1108 2 3.6388995202944123 1108 3 2.130568592525467 1108 4 0.9443050189229325 1108 5 0.2675450174710292 1108 6 0.050896537985309465 1108 7 0.03393818471176502 1108 8 0.027649929062604883 1108 9 -0.02016282539920411 1108 10-11 0.16064449027542338 1108 12-13 0.07731231530318183 1108 14-15 0.031518190552731085 1108 16-17 -0.009079123701567937 1108 18-19 0.08102247826536058 1108 20-21 0.03919076608826799 1108 22-23 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-0.027893799144443676 2211 80-81 0.02519217593835066 2211 82-83 -0.21373888604580316 2211 84-85 -0.040525110500631456 2211 86-87 0.048940743105724493 2211 88-89 0.06391749618572362 2211 90-91 0.017977008998201427 2211 92-93 0.10749728486693044 2211 94-95 0.25934128819489644 2211 96-97 0.20105847071545924 2211 98-99 0.23616081117316412 2211 100 0.15966433682043402 2212 1 0.5030501558656582 2212 2 4.06820550763824 2212 3 2.282169825691131 2212 4 1.0946924709214159 2212 5 0.6133347951766908 2212 6 0.52269109691224 2212 7 0.4830510626391842 2212 8 0.33678976355879087 2212 9 0.3895603180915188 2212 10-11 0.39252316408146726 2212 12-13 0.4406658768803382 2212 14-15 0.6436539685829104 2212 16-17 0.5968868885219223 2212 18-19 0.5408892963734289 2212 20-21 0.4839430836825116 2212 22-23 0.4771111006392772 2212 24-25 0.5149509184397374 2212 26-27 0.39238969696327075 2212 28-29 0.46021748740238877 2212 30-31 0.3775335636377477 2212 32-33 0.5530625410656 2212 34-35 0.5723690011216505 2212 36-37 0.440206189550274 2212 38-39 0.3549955680086043 2212 40-41 0.4596984359752554 2212 42-43 0.49942193901116383 2212 44-45 0.5807925108969343 2212 46-47 0.5199150826127834 2212 48-49 0.41864762606704176 2212 50-51 0.4715871505731286 2212 52-53 0.4673580374876991 2212 54-55 0.40483837456962135 2212 56-57 0.5901777237864039 2212 58-59 0.5264784328769778 2212 60-61 0.4945526152365147 2212 62-63 0.6133555200571763 2212 64-65 0.6023173550288661 2212 66-67 0.4107458881639232 2212 68-69 0.4886699965491488 2212 70-71 0.498460153669825 2212 72-73 0.5040857328095285 2212 74-75 0.5975634276268948 2212 76-77 0.5067391895518014 2212 78-79 0.6189898166747696 2212 80-81 0.41879104599484407 2212 82-83 0.5397503794909255 2212 84-85 0.7162816691603808 2212 86-87 0.6362695566650345 2212 88-89 0.7408361402535206 2212 90-91 0.6705408508061055 2212 92-93 0.599463386561844 2212 94-95 0.618244113053656 2212 96-97 0.5333454763651773 2212 98-99 0.5275879298172299 2212 100 0.6188846758034856 2213 1 -0.4407161362691703 2213 2 -26.037394492361763 2213 3 -14.903843657454935 2213 4 -5.945385431700306 2213 5 -2.0000045306660112 2213 6 -1.1202632102038663 2213 7 -0.9841549298701793 2213 8 -0.7990124836322181 2213 9 -1.0292921163654114 2213 10-11 -0.9912760868548602 2213 12-13 -0.8944352467151688 2213 14-15 -1.1295864808552878 2213 16-17 -1.2807790290810743 2213 18-19 -1.0125668834018597 2213 20-21 -1.0530486766171165 2213 22-23 -1.0337802851285147 2213 24-25 -0.9288123774404085 2213 26-27 -1.179655246856953 2213 28-29 -1.2746649096013556 2213 30-31 -1.3797458371113152 2213 32-33 -1.1977659233538773 2213 34-35 -1.1158647067435155 2213 36-37 -1.1689533610115248 2213 38-39 -1.183095817759181 2213 40-41 -1.1672468823768014 2213 42-43 -1.1854469748465064 2213 44-45 -1.003423219440144 2213 46-47 -1.0263321083984493 2213 48-49 -1.12008795445729 2213 50-51 -1.1263963700261144 2213 52-53 -1.273645707830653 2213 54-55 -1.2109668688760706 2213 56-57 -1.1927855720937401 2213 58-59 -1.1336204435275121 2213 60-61 -1.409669107609929 2213 62-63 -1.1377965398679137 2213 64-65 -1.1638788996527794 2213 66-67 -1.3232795800008645 2213 68-69 -1.2352341233010335 2213 70-71 -1.1635653144949671 2213 72-73 -1.3122828065163148 2213 74-75 -1.3182508045828456 2213 76-77 -1.1782825332946416 2213 78-79 -1.2072513818271062 2213 80-81 -1.2233512761025302 2213 82-83 -1.29766385271882 2213 84-85 -1.1615490424501047 2213 86-87 -1.3007761362188575 2213 88-89 -1.2175968185479817 2213 90-91 -0.946705591141459 2213 92-93 -1.065060958007443 2213 94-95 -1.0683356622272449 2213 96-97 -1.2395414150205895 2213 98-99 -1.2413618829168556 2213 100 -1.2031078335598089 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 27.0 5 50.0 6 37.0 7 43.0 8 45.0 9 79.0 10 325.0 11 1369.0 12 3809.0 13 6767.0 14 9237.0 15 10732.0 16 11884.0 17 12290.0 18 13042.0 19 14007.0 20 15883.0 21 17382.0 22 19900.0 23 21197.0 24 22358.0 25 24615.0 26 27221.0 27 31432.0 28 37062.0 29 44775.0 30 55015.0 31 68610.0 32 88024.0 33 119304.0 34 167041.0 35 242198.0 36 376262.0 37 652463.0 38 634717.0 39 3715.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.96349486767786 20.297810804441916 10.227979608295437 30.51071471958479 2 13.144029329944573 22.458824456744292 36.587106436400205 27.810039776910934 3 15.606521933390146 26.744670942844085 28.84040471847046 28.808402405295315 4 10.675361002633728 18.361020797384434 37.28300790118972 33.68061029879212 5 11.759199159399705 38.495193035358774 35.39311452887963 14.3524932763619 6 30.442513063354316 39.30276924575462 17.460063044291285 12.794654646599785 7 26.257097330404466 32.97721768295896 23.124878493703477 17.640806492933102 8 24.467854304390283 37.143048191015346 20.692566186376098 17.696531318218277 9 24.885837779536637 16.65267575312906 21.597100937354867 36.86438552997944 10-11 23.23744761511592 27.419262228954107 29.171148666229612 20.172141489700365 12-13 24.060691239090083 24.941351970848146 29.348198768259653 21.649758021802114 14-15 21.615287012148578 26.508133181355742 26.282438102838533 25.594141703657147 16-17 21.03202339412912 29.969303835703297 27.293241484062797 21.705431286104783 18-19 21.01354895287525 29.01850801823968 29.18579653624836 20.78214649263671 20-21 22.119371879170416 28.382522373325614 28.423506679751608 21.074599067752363 22-23 21.37470812745036 28.353398274843162 28.51337522479159 21.75851837291488 24-25 21.54261377777116 28.20936279797599 28.345028141293966 21.902995282958884 26-27 21.479137141239953 28.13789362744867 28.476177362909077 21.906791868402305 28-29 21.203505073098654 28.084873241109932 28.897197565552084 21.81442412023933 30-31 21.82348715813431 28.4699233764502 28.232575309188324 21.474014156227167 32-33 20.96667091675957 28.441857820951945 28.879708203878046 21.711763058410444 34-35 21.09133344836668 28.050875010216775 28.922379737179078 21.935411804237464 36-37 21.74603880954046 28.435585190001827 28.28315870826185 21.535217292195863 38-39 21.54575550058832 28.42966908376384 28.4649973789856 21.559578036662234 40-41 21.549748513339757 28.28622344250554 28.403161878723687 21.760866165431015 42-43 21.43373127415231 28.241670715703147 28.578683752843787 21.745914257300754 44-45 21.702119930177265 27.925262062313706 28.493380099793477 21.879237907715556 46-47 21.60104779473541 28.464902600322077 28.4358920560701 21.498157548872413 48-49 21.54562809206281 28.31016042392145 28.482440280676464 21.661771203339285 50-51 21.696782769303212 28.24213241154146 28.21384718821809 21.84723763093724 52-53 21.356425498517527 28.39037337945468 28.172707982094064 22.080493139933726 54-55 21.635382325514474 28.399616242024038 27.98842621987675 21.976575212584734 56-57 21.75214155741274 28.130028961002466 28.10147304563188 22.016356435952915 58-59 21.314915776224574 27.85837770027704 28.64015264565888 22.18655387783951 60-61 21.88529033083096 28.23746704914364 27.993187492189158 21.884055127836245 62-63 21.26473384703866 28.442051561152894 28.295199026602397 21.99801556520605 64-65 21.24554860820831 27.958528097335765 28.457570831241952 22.33835246321397 66-67 21.561190663389947 28.165424575671388 28.16057417158005 22.112810589358613 68-69 21.72586648683138 28.064043067451315 28.19960990469989 22.010480541017415 70-71 21.75160606340828 28.04220048445787 27.989078350968345 22.217115101165508 72-73 21.525683147469746 28.248183072530853 28.227599857207352 21.998533922792053 74-75 21.811576246392914 27.83674427426276 28.023883974475893 22.32779550486843 76-77 21.559974763874557 28.2103986570283 28.188726689237242 22.0408998898599 78-79 21.674272471274328 28.17815275769 28.250559581588103 21.89701518944757 80-81 21.691239447237322 27.997856523298957 28.120833957757164 22.19007007170656 82-83 21.233006597919267 28.21395175475881 28.255365849937114 22.29767579738481 84-85 21.44490659218697 27.894828429921166 28.051806887071056 22.60845809082081 86-87 21.60171615039566 28.138618015764678 27.93407134339446 22.325594490445194 88-89 21.321968785334466 27.787645822075497 28.370271141646647 22.520114250943386 90-91 21.51552447999558 27.868011858828933 28.13055246870637 22.485911192469114 92-93 21.092430900050278 28.204382118144856 28.227195054969005 22.47599192683586 94-95 21.03541237610536 27.78921523843912 28.41617374591498 22.759198639540543 96-97 21.322552235284586 27.926086793058026 28.199470777137382 22.551890194520006 98-99 21.257794011075532 27.810484522769503 28.192352470018143 22.73936899613682 100 21.313047418247724 27.731845696328012 28.100870050917216 22.85423683450705 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10.0 1 9.0 2 9.5 3 11.5 4 15.0 5 33.0 6 72.5 7 142.0 8 204.0 9 222.5 10 198.5 11 179.5 12 193.5 13 223.5 14 268.0 15 323.5 16 417.5 17 500.5 18 600.5 19 755.0 20 988.5 21 1413.0 22 2045.0 23 3075.0 24 4725.5 25 7039.0 26 10700.0 27 16019.5 28 22688.0 29 30696.0 30 39841.5 31 49883.0 32 60056.5 33 69792.5 34 79211.0 35 87947.0 36 95134.5 37 101665.0 38 107693.5 39 112053.0 40 114409.0 41 114688.5 42 113274.0 43 110693.5 44 107166.0 45 103148.5 46 99389.5 47 95548.0 48 91488.5 49 90141.0 50 97459.5 51 93510.0 52 77011.5 53 68380.0 54 63633.0 55 59129.0 56 54015.5 57 48663.5 58 43418.5 59 38396.0 60 33214.0 61 27868.0 62 22733.5 63 18251.5 64 14563.5 65 11562.0 66 9014.5 67 6830.5 68 5112.5 69 3835.5 70 2869.5 71 2086.0 72 1489.5 73 1047.5 74 725.5 75 473.0 76 278.5 77 162.5 78 90.5 79 44.0 80 25.0 81 12.0 82 7.0 83 6.0 84 4.0 85 1.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.728315980887245 2 5.5276095325482775 3 0.0 4 0.006393197637713472 5 0.16033849075492768 6 0.05546825450447996 7 0.036724561430274555 8 0.04319040904114386 9 0.041410484698825904 10-11 0.04279083418878677 12-13 0.040992747353179856 14-15 0.04375344633310158 16-17 0.034635874702044295 18-19 0.028860201836155418 20-21 0.0237202162353801 22-23 0.023774703715246977 24-25 0.020523617416523365 26-27 0.012895370235160698 28-29 0.01872553058091645 30-31 0.023211666423289257 32-33 0.0016346243960062858 34-35 0.003668823644369663 36-37 0.0044861358423728065 38-39 0.006356872651135555 40-41 0.007101534875982863 42-43 0.007755384634385377 44-45 0.008754321765278107 46-47 0.017381506077533505 48-49 0.026081340362944735 50-51 0.02199477937292902 52-53 0.027824939718684775 54-55 0.02435590350049366 56-57 0.015674231708371383 58-59 0.014802432030501364 60-61 0.012423145409647772 62-63 0.018344118221848317 64-65 0.020214855030611066 66-67 0.020995842242036294 68-69 0.028260839557619783 70-71 0.028242677064330823 72-73 0.024828128326006584 74-75 0.02557279055085389 76-77 0.018979805486961873 78-79 0.015365469322459085 80-81 0.014112257285520933 82-83 0.00871799677870019 84-85 0.01158767071835567 86-87 0.009208384097502076 88-89 0.007755384634385377 90-91 0.007591922194784748 92-93 0.003705148630947581 94-95 0.0011805620637823173 96-97 7.809872114252253E-4 98-99 3.99574852357092E-4 100 0.10795786010957069 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2752926.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.06124679529165 #Duplication Level Percentage of deduplicated Percentage of total 1 87.57922905476116 71.86860729604132 2 9.152459346457784 15.021244504270918 3 1.9506871599623437 4.802274613622293 4 0.6141464096886009 2.0159048037559426 5 0.2584240767971221 1.0603300971947018 6 0.13772325213481665 0.6781045069731221 7 0.07694013289353066 0.4419662263686989 8 0.0522192662890235 0.34281424787300885 9 0.03796345992031707 0.28037959683518715 >10 0.13369121405840517 1.7880653158837818 >50 0.003936411649894039 0.2171882690340436 >100 0.0016040437503703213 0.30851370935620764 >500 4.8808581862735083E-4 0.24792764172633686 >1k 3.9934294204728985E-4 0.4888642259611473 >5k 8.874287601050887E-5 0.4378149451032948 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6854 0.24897145800504625 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5170 0.18780018060783327 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.632498657791746E-5 0.0 3.632498657791746E-5 0.0 5 0.0 3.632498657791746E-5 0.0 7.264997315583492E-5 0.0 6 0.0 3.632498657791746E-5 0.0 1.0897495973375238E-4 0.0 7 0.0 3.632498657791746E-5 0.0 1.4529994631166983E-4 0.0 8 0.0 3.632498657791746E-5 0.0 1.4529994631166983E-4 0.0 9 0.0 3.632498657791746E-5 0.0 1.816249328895873E-4 0.0 10-11 0.0 3.632498657791746E-5 0.0 2.1794991946750476E-4 0.0 12-13 0.0 3.632498657791746E-5 0.0 2.542749060454222E-4 0.0 14-15 0.0 3.632498657791746E-5 0.0 3.2692487920125713E-4 0.0 16-17 0.0 3.632498657791746E-5 0.0 3.632498657791746E-4 0.0 18-19 0.0 3.632498657791746E-5 0.0 3.632498657791746E-4 0.0 20-21 3.632498657791746E-5 3.632498657791746E-5 0.0 4.177373456460508E-4 0.0 22-23 3.632498657791746E-5 3.632498657791746E-5 0.0 4.358998389350095E-4 0.0 24-25 3.632498657791746E-5 3.632498657791746E-5 0.0 4.358998389350095E-4 0.0 26-27 3.632498657791746E-5 3.632498657791746E-5 0.0 6.901747449804317E-4 0.0 28-29 3.632498657791746E-5 3.632498657791746E-5 0.0 9.807746376037714E-4 0.0 30-31 3.632498657791746E-5 3.632498657791746E-5 0.0 0.0032874112853015303 0.0 32-33 3.632498657791746E-5 3.632498657791746E-5 0.0 0.009262871577368952 0.0 34-35 3.632498657791746E-5 3.632498657791746E-5 0.0 0.019106942939984585 0.0 36-37 3.632498657791746E-5 3.632498657791746E-5 0.0 0.03358245009128469 0.0 38-39 3.632498657791746E-5 3.632498657791746E-5 0.0 0.05501419217225599 0.0 40-41 3.632498657791746E-5 3.632498657791746E-5 0.0 0.08263934446476223 0.0 42-43 3.632498657791746E-5 3.632498657791746E-5 0.0 0.11060958412975866 0.0 44-45 3.632498657791746E-5 3.632498657791746E-5 0.0 0.14310228462370583 0.0 46-47 3.632498657791746E-5 3.632498657791746E-5 0.0 0.18507580661448947 0.0 48-49 3.632498657791746E-5 3.632498657791746E-5 0.0 0.22797561576300998 0.0 50-51 3.632498657791746E-5 7.264997315583492E-5 0.0 0.27652396032439663 0.0 52-53 3.632498657791746E-5 9.081246644479365E-5 0.0 0.3272336415871694 0.0 54-55 3.632498657791746E-5 1.0897495973375238E-4 0.0 0.38371899571583107 0.0 56-57 3.632498657791746E-5 1.0897495973375238E-4 0.0 0.45159223313666985 0.0 58-59 3.632498657791746E-5 1.0897495973375238E-4 0.0 0.529454841866436 0.0 60-61 5.4487479866876184E-5 1.0897495973375238E-4 0.0 0.6113858490929287 0.0 62-63 7.264997315583492E-5 1.271374530227111E-4 0.0 0.7001096288094921 0.0 64-65 7.264997315583492E-5 1.4529994631166983E-4 0.0 0.7990044047678724 0.0 66-67 7.264997315583492E-5 1.4529994631166983E-4 0.0 0.9125563128104424 0.0 68-69 7.264997315583492E-5 1.4529994631166983E-4 0.0 1.0407471904439132 0.0 70-71 7.264997315583492E-5 1.4529994631166983E-4 0.0 1.1898612603462644 0.0 72-73 7.264997315583492E-5 1.4529994631166983E-4 0.0 1.3407734170842223 0.0 74-75 7.264997315583492E-5 1.4529994631166983E-4 0.0 1.5035093569532927 0.0 76-77 7.264997315583492E-5 1.4529994631166983E-4 0.0 1.687241139064399 0.0 78-79 7.264997315583492E-5 1.4529994631166983E-4 0.0 1.8899890516490454 0.0 80-81 7.264997315583492E-5 1.4529994631166983E-4 0.0 2.1141142188347963 0.0 82-83 7.264997315583492E-5 1.4529994631166983E-4 0.0 2.35667431670884 0.0 84-85 7.264997315583492E-5 1.4529994631166983E-4 0.0 2.60609983704611 0.0 86-87 7.264997315583492E-5 1.4529994631166983E-4 0.0 2.878173986514712 0.0 88 7.264997315583492E-5 1.4529994631166983E-4 0.0 3.103643178203846 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 295 3.417881E-9 19.131392 5 CCGTACA 195 1.5909212E-4 17.869698 2 CATGGGG 4245 0.0 16.618847 4 TAGAGCG 180 0.0027989408 15.677113 5 ACCGTCC 240 6.796937E-5 15.649762 8 GAGTACT 2935 0.0 15.435077 12-13 TACCGTT 220 6.23218E-4 14.938411 7 GTACATG 13200 0.0 14.794801 1 GTATAGA 1015 0.0 14.7248955 1 GTACCGT 225 7.3816255E-4 14.60857 6 GTATTAG 1095 0.0 14.559044 1 ACACCGT 360 6.954524E-7 14.347701 6 GTATATA 1885 0.0 14.271821 1 TACATGG 13430 0.0 14.159278 2 CTATGCG 300 2.868728E-5 14.084531 9 GTACGGA 320 3.0122703E-5 14.011658 1 TATACTG 1580 0.0 13.990335 5 GTATAAG 1005 0.0 13.879984 1 ACATGGG 13230 0.0 13.863609 3 GTATAGG 725 0.0 13.743236 1 >>END_MODULE