FastQCFastQC Report
Wed 25 May 2016
SRR1295115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717866
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG46200.022.2141511
GTATCAA28600.022.0005511
TACATGG48050.021.1012552
ATCAACG26300.020.0551073
TCAACGC26400.019.7678854
ACATGGG50300.019.0956863
GTACTAA7750.018.9178581
CAACGCA25900.018.5247975
TATCAAC31750.017.8909262
AACGCAG27450.017.1606086
GTATTAG9800.016.830621
CGCAGAG27800.016.7755648
ACGCAGA28450.016.4948837
GTATAGG7450.015.5797221
CATGGGG29100.014.4996284
GTCTTAC8450.014.4589141
GTGTACG2550.00498501314.3738631
ATTATAC14350.014.31551553
GTGTATA14250.014.1469071
TAACGGT2759.487141E-414.1319354
Produced by FastQC (version 0.11.5)