##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295115_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2717866 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.317052422746375 27.0 27.0 33.0 15.0 33.0 2 22.689227872161467 27.0 15.0 33.0 2.0 33.0 3 24.91858575809109 27.0 15.0 33.0 15.0 33.0 4 25.42978535365614 33.0 15.0 37.0 2.0 37.0 5 27.016721942877243 33.0 15.0 37.0 2.0 37.0 6 27.730083823117106 33.0 15.0 37.0 6.0 37.0 7 27.57326520144849 33.0 15.0 37.0 6.0 37.0 8 28.211992055531802 33.0 22.0 37.0 6.0 37.0 9 29.304826286505662 33.0 27.0 37.0 15.0 37.0 10-11 28.83591262409552 33.0 24.5 37.0 10.5 37.0 12-13 27.626910414273553 33.0 15.0 37.0 4.0 37.0 14-15 29.35446467927411 33.0 24.5 37.0 10.5 40.0 16-17 29.813045602689755 33.0 27.0 37.0 10.5 40.0 18-19 29.93160718740365 33.0 27.0 38.5 10.5 40.0 20-21 29.465322977659678 33.0 24.5 38.5 6.0 40.0 22-23 30.177547016666754 33.0 27.0 37.0 15.0 40.0 24-25 30.275012822560054 33.0 27.0 37.0 15.0 40.0 26-27 28.950724943761024 33.0 22.0 37.0 4.0 40.0 28-29 29.618175620137272 33.0 27.0 37.0 10.5 40.0 30-31 30.30452347540313 33.0 27.0 37.0 15.0 40.0 32-33 29.138251297157403 33.0 24.5 37.0 6.0 40.0 34-35 28.672184353459663 33.0 22.0 37.0 6.0 40.0 36-37 28.939970366456624 33.0 22.0 37.0 6.0 40.0 38-39 29.07708713380277 33.0 24.5 37.0 6.0 40.0 40-41 28.24109687526905 33.0 22.0 37.0 6.0 40.0 42-43 28.83718549773977 33.0 22.0 37.0 6.0 40.0 44-45 28.679681411813533 33.0 22.0 37.0 6.0 40.0 46-47 28.42338603154092 33.0 22.0 37.0 6.0 40.0 48-49 28.244372055134434 33.0 22.0 37.0 6.0 40.0 50-51 28.194858208609254 33.0 22.0 37.0 6.0 40.0 52-53 28.405813421265066 33.0 22.0 37.0 6.0 40.0 54-55 28.396486434577717 33.0 22.0 37.0 6.0 40.0 56-57 28.374153471878305 33.0 22.0 37.0 6.0 40.0 58-59 28.192533406724245 33.0 22.0 37.0 6.0 40.0 60-61 28.165977093793437 33.0 22.0 37.0 6.0 40.0 62-63 28.074468903176243 33.0 22.0 37.0 6.0 40.0 64-65 27.68436284202385 33.0 22.0 37.0 6.0 40.0 66-67 27.922137625622454 33.0 22.0 37.0 10.5 40.0 68-69 27.50748418060346 33.0 22.0 37.0 6.0 38.5 70-71 27.62489890965927 33.0 22.0 37.0 10.5 37.0 72-73 27.625512258514583 33.0 22.0 37.0 10.5 37.0 74-75 27.725466229755256 33.0 22.0 37.0 15.0 37.0 76-77 27.514242424019432 30.0 22.0 37.0 10.5 37.0 78-79 27.085103717401815 27.0 22.0 35.0 6.0 37.0 80-81 26.88893363396135 27.0 22.0 33.0 6.0 37.0 82-83 26.952524517397105 27.0 22.0 33.0 10.5 37.0 84-85 26.89271638116081 27.0 22.0 33.0 10.5 37.0 86-87 26.67636281553248 27.0 22.0 33.0 10.5 37.0 88-89 26.613795345318717 27.0 22.0 33.0 10.5 37.0 90-91 26.723715959506464 27.0 22.0 33.0 15.0 37.0 92-93 26.665888053347736 27.0 22.0 33.0 15.0 37.0 94-95 26.547775534187487 27.0 22.0 33.0 15.0 37.0 96-97 26.630670901361583 27.0 22.0 33.0 15.0 37.0 98-99 26.699713856385856 27.0 22.0 33.0 15.0 37.0 100 26.376536223640166 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.285310030161604 1101 2 11.253562603101905 1101 3 8.455460297607516 1101 4 12.782960963882164 1101 5 10.702016178567401 1101 6 9.960194782187386 1101 7 9.45282604496514 1101 8 9.040381537279831 1101 9 7.448160569900221 1101 10-11 7.7319089959555605 1101 12-13 9.108560827826203 1101 14-15 7.93019489279072 1101 16-17 8.178058140370343 1101 18-19 8.1870799017697 1101 20-21 7.165505388519673 1101 22-23 7.363174893153385 1101 24-25 7.332741236960047 1101 26-27 8.496605809082784 1101 28-29 8.180041938342452 1101 30-31 5.508796490594058 1101 32-33 7.035155686656967 1101 34-35 8.798085624105514 1101 36-37 8.177962564477173 1101 38-39 8.217490937050869 1101 40-41 8.943514792528521 1101 42-43 8.563426218683041 1101 44-45 8.38434247939432 1101 46-47 7.416288110591427 1101 48-49 5.509995976532796 1101 50-51 6.784594776348872 1101 52-53 5.938647033622228 1101 54-55 4.092564031818515 1101 56-57 6.235121015813803 1101 58-59 6.20920315327384 1101 60-61 6.819956920036301 1101 62-63 6.395789609544966 1101 64-65 6.915900524171462 1101 66-67 5.828656960210264 1101 68-69 6.78413664872933 1101 70-71 6.383646645780377 1101 72-73 5.64984098402784 1101 74-75 5.066037322198543 1101 76-77 5.179468100202971 1101 78-79 5.276402196359864 1101 80-81 5.035379875029083 1101 82-83 4.572496753734967 1101 84-85 4.276523610663656 1101 86-87 4.064572060905494 1101 88-89 4.140252739785716 1101 90-91 4.003692212232028 1101 92-93 3.9194509375972615 1101 94-95 3.7197082110657327 1101 96-97 3.8211714286634333 1101 98-99 3.538624543253775 1101 100 3.832200142940227 1102 1 6.680499517725668 1102 2 10.518228423079108 1102 3 7.996334319035853 1102 4 12.55244529385585 1102 5 10.402585839545015 1102 6 9.442555063253764 1102 7 6.721096323670011 1102 8 7.786267588091182 1102 9 6.349885197302232 1102 10-11 7.4541932475990045 1102 12-13 9.07677529893322 1102 14-15 6.1331678409410415 1102 16-17 7.234450086112087 1102 18-19 7.4123449642578905 1102 20-21 6.9216057672102345 1102 22-23 6.42033755827503 1102 24-25 6.967547159902132 1102 26-27 8.394248834933517 1102 28-29 7.629165193967278 1102 30-31 4.11719349759132 1102 32-33 4.426254563613352 1102 34-35 4.9719327245042955 1102 36-37 4.874820372840787 1102 38-39 4.520520110883986 1102 40-41 6.282521916291511 1102 42-43 6.883905584100212 1102 44-45 6.435184751990839 1102 46-47 5.4489191690975645 1102 48-49 3.532021082938787 1102 50-51 4.904340227348573 1102 52-53 5.605347605629877 1102 54-55 5.174320625186795 1102 56-57 6.157903071769482 1102 58-59 5.9197019947064895 1102 60-61 5.839765299564643 1102 62-63 4.220132943065767 1102 64-65 6.137397292577077 1102 66-67 3.737286179682073 1102 68-69 5.988073201775343 1102 70-71 5.209350346816727 1102 72-73 4.913865488661234 1102 74-75 4.1795799164912 1102 76-77 3.2701704842717234 1102 78-79 4.280733421600978 1102 80-81 4.399179948962686 1102 82-83 3.8185887181286944 1102 84-85 2.8953282896007124 1102 86-87 3.29979678359042 1102 88-89 3.401479271564689 1102 90-91 3.459592247310706 1102 92-93 3.139779832891392 1102 94-95 2.9828574615945094 1102 96-97 3.302413615700882 1102 98-99 3.1559236308440575 1102 100 3.312915758723392 1103 1 6.666946951301931 1103 2 11.022490697588982 1103 3 7.9749731979697 1103 4 12.074860520805014 1103 5 10.379469179094738 1103 6 9.1564666726143 1103 7 8.101536035782413 1103 8 8.118192546632482 1103 9 7.1500091061045445 1103 10-11 7.548076425236179 1103 12-13 8.812258400398214 1103 14-15 8.659548209948507 1103 16-17 8.119378555430234 1103 18-19 7.344715348374358 1103 20-21 8.368946269142437 1103 22-23 6.977439646924985 1103 24-25 5.2432246475551025 1103 26-27 6.003181673118373 1103 28-29 7.039823815747543 1103 30-31 6.27298618606622 1103 32-33 7.987340623277479 1103 34-35 7.254921336526717 1103 36-37 4.2685480029913485 1103 38-39 6.515075729272866 1103 40-41 6.754958259942132 1103 42-43 7.554842023007232 1103 44-45 5.665686318321171 1103 46-47 6.396977614176588 1103 48-49 5.666535308365905 1103 50-51 6.566123378326029 1103 52-53 6.217250423405083 1103 54-55 5.072793358072779 1103 56-57 6.119923146455662 1103 58-59 5.5972816378275105 1103 60-61 5.055478738446443 1103 62-63 2.697169384533641 1103 64-65 5.127265749813404 1103 66-67 4.114173188956869 1103 68-69 5.26913883119893 1103 70-71 3.7471309486939113 1103 72-73 4.480584795891797 1103 74-75 3.353085821688172 1103 76-77 3.581274473987488 1103 78-79 3.050653461942151 1103 80-81 4.371420619688518 1103 82-83 3.6576416990502096 1103 84-85 3.4977375395915224 1103 86-87 2.9370689021034906 1103 88-89 3.1920706646403616 1103 90-91 2.876990139406349 1103 92-93 2.4419985453767197 1103 94-95 2.9523164697361644 1103 96-97 3.109757633103449 1103 98-99 2.8978950779676182 1103 100 3.0594788635710053 1104 1 6.3453771096314355 1104 2 9.653049126427874 1104 3 7.274270203045429 1104 4 12.195923200454889 1104 5 9.499015439598775 1104 6 8.079406587973565 1104 7 3.8532568106226925 1104 8 6.374895633033287 1104 9 6.610385780301588 1104 10-11 7.1448417406203895 1104 12-13 7.39927471997688 1104 14-15 7.190586216154454 1104 16-17 7.081810547031171 1104 18-19 6.823814036397337 1104 20-21 7.556970029351259 1104 22-23 6.480490026721235 1104 24-25 6.0318648343712695 1104 26-27 8.203121640932121 1104 28-29 7.204322718014918 1104 30-31 6.401651804380023 1104 32-33 7.690718357587844 1104 34-35 8.057704184290518 1104 36-37 6.589703309720139 1104 38-39 7.16391917876922 1104 40-41 7.025139328793333 1104 42-43 7.253403523281399 1104 44-45 6.735077959955774 1104 46-47 6.654215957343791 1104 48-49 5.375439480940077 1104 50-51 5.59943208837451 1104 52-53 5.815896491301132 1104 54-55 4.741520598927018 1104 56-57 5.954229934108266 1104 58-59 3.7289116428913083 1104 60-61 4.18484964426565 1104 62-63 3.766470768184284 1104 64-65 2.6880211051369827 1104 66-67 3.1249852594503693 1104 68-69 4.058799925079786 1104 70-71 2.4699255982913044 1104 72-73 2.28949256178225 1104 74-75 2.919380622627095 1104 76-77 3.6466408079068344 1104 78-79 4.299613915257705 1104 80-81 3.006626810439716 1104 82-83 2.815056709822933 1104 84-85 2.8190160194215004 1104 86-87 0.7373329003304114 1104 88-89 0.2908442697598943 1104 90-91 2.2481434100170468 1104 92-93 2.3953332406562744 1104 94-95 2.301497333898695 1104 96-97 2.740609478320952 1104 98-99 2.474145207013862 1104 100 0.6608387070769801 1105 1 5.718627988780494 1105 2 -2.5404592181078556 1105 3 0.5678264661606462 1105 4 8.564918396332065 1105 5 8.593456000166292 1105 6 8.098686400246827 1105 7 5.3429251370406305 1105 8 5.939288163781757 1105 9 4.836281494070221 1105 10-11 4.9252062327248005 1105 12-13 4.914857385658934 1105 14-15 5.819335310193811 1105 16-17 5.986762133578257 1105 18-19 6.254562176628781 1105 20-21 6.933433121919403 1105 22-23 1.8955430209621653 1105 24-25 3.923272861931302 1105 26-27 6.8825874029644645 1105 28-29 5.993541822011647 1105 30-31 5.297434712678417 1105 32-33 7.1243914136836715 1105 34-35 6.031464469314791 1105 36-37 6.2187691655359885 1105 38-39 4.080141129642978 1105 40-41 5.274352251863764 1105 42-43 5.579374423583083 1105 44-45 6.129643123091441 1105 46-47 5.304180931144021 1105 48-49 4.932405915401297 1105 50-51 5.8357282873758045 1105 52-53 5.542315059522501 1105 54-55 5.281955750970102 1105 56-57 5.164187457710305 1105 58-59 1.9599388945867844 1105 60-61 4.340020786327983 1105 62-63 -0.9100925112948381 1105 64-65 -0.5162087505910158 1105 66-67 0.744021742317905 1105 68-69 2.7890077120200907 1105 70-71 3.0093897002871444 1105 72-73 2.684319665557638 1105 74-75 2.6919464372272586 1105 76-77 1.6217652322064566 1105 78-79 3.2105681453130934 1105 80-81 1.5073646282104924 1105 82-83 0.8934989538157829 1105 84-85 1.2968551738639356 1105 86-87 -0.31137038868108746 1105 88-89 1.5272806306061923 1105 90-91 1.2851296936088978 1105 92-93 1.663844473142106 1105 94-95 0.9655610519183888 1105 96-97 2.086044492027664 1105 98-99 2.0143105888636157 1105 100 2.1227003381854956 1106 1 5.953444147810568 1106 2 10.316057072964984 1106 3 6.689252406516637 1106 4 11.709267700525789 1106 5 9.827295340957566 1106 6 9.316278903354608 1106 7 6.571091443548834 1106 8 7.450490371452826 1106 9 6.938328074074274 1106 10-11 5.428437552760347 1106 12-13 7.811757307626131 1106 14-15 7.699733200160377 1106 16-17 7.602943999153087 1106 18-19 7.1455300318644035 1106 20-21 8.063399070238827 1106 22-23 6.072819654142286 1106 24-25 4.900570425352974 1106 26-27 7.6132570547216964 1106 28-29 6.010768349629348 1106 30-31 5.170873352555098 1106 32-33 7.347804070239395 1106 34-35 6.551413667918485 1106 36-37 5.616321818347462 1106 38-39 6.994623089597184 1106 40-41 6.509248487015853 1106 42-43 5.945773792472792 1106 44-45 4.099017016177115 1106 46-47 5.6991905043824715 1106 48-49 5.635950968160415 1106 50-51 6.48157344114129 1106 52-53 5.3591164933821105 1106 54-55 5.043021580721643 1106 56-57 4.754837797315901 1106 58-59 4.8389888549076865 1106 60-61 4.610669215151631 1106 62-63 4.648554826284553 1106 64-65 5.015702301957145 1106 66-67 2.9891616187356007 1106 68-69 2.9301702902945017 1106 70-71 2.6150927769222 1106 72-73 2.511867094092459 1106 74-75 2.5502889885187763 1106 76-77 3.398101136627602 1106 78-79 4.008622654509974 1106 80-81 3.5297572254215233 1106 82-83 1.8458150866140244 1106 84-85 2.6492886872120742 1106 86-87 3.169157390277885 1106 88-89 3.2135282469609407 1106 90-91 3.036833685872992 1106 92-93 2.766035328228071 1106 94-95 1.2361523781833021 1106 96-97 1.3056456080126821 1106 98-99 1.8299815963924289 1106 100 2.719712615235622 1107 1 6.079239513240552 1107 2 10.430745081871851 1107 3 6.047548436216346 1107 4 11.337236553184557 1107 5 9.294448009804949 1107 6 7.953667313073858 1107 7 3.54266028563886 1107 8 6.109430079480841 1107 9 5.5369020881736155 1107 10-11 4.393555752504167 1107 12-13 5.871714083345559 1107 14-15 5.986822921556531 1107 16-17 6.7274436318120685 1107 18-19 6.06475459893899 1107 20-21 5.329449340935977 1107 22-23 5.679983460436212 1107 24-25 4.47968108830381 1107 26-27 7.020690386008571 1107 28-29 6.2670984250210005 1107 30-31 4.905463935971191 1107 32-33 4.557144309330159 1107 34-35 6.384643409925889 1107 36-37 6.293840103898884 1107 38-39 6.267442237069254 1107 40-41 7.517623525786757 1107 42-43 5.985843295191955 1107 44-45 3.9400552991293765 1107 46-47 3.255546778230059 1107 48-49 3.7834487991136037 1107 50-51 4.707003087268799 1107 52-53 3.6162870761373753 1107 54-55 5.072234307053932 1107 56-57 4.987590241461671 1107 58-59 2.72422178728522 1107 60-61 2.58451470830288 1107 62-63 0.1897299374921566 1107 64-65 1.764867917403528 1107 66-67 3.91762136906506 1107 68-69 4.733041582043587 1107 70-71 4.100436907251783 1107 72-73 2.171393447301039 1107 74-75 2.391234395062604 1107 76-77 2.872218101935701 1107 78-79 3.8340577560060325 1107 80-81 2.0573351476939408 1107 82-83 1.4882797471061409 1107 84-85 2.1786671844880594 1107 86-87 2.1017480653706464 1107 88-89 2.3362070331508136 1107 90-91 1.0895694621854481 1107 92-93 2.0306600874044385 1107 94-95 2.036752796928347 1107 96-97 2.163545916640917 1107 98-99 1.5718924404460566 1107 100 -0.06409021902967282 1108 1 3.986844838536946 1108 2 -3.2645148114492955 1108 3 -0.18044218460847006 1108 4 6.430017017490787 1108 5 6.5682043118242674 1108 6 6.351860895461758 1108 7 4.017845614929019 1108 8 4.653291475199278 1108 9 4.52608383954313 1108 10-11 2.8394055498308184 1108 12-13 1.999570315988631 1108 14-15 3.4046374255163308 1108 16-17 4.20716637384152 1108 18-19 3.569177962884332 1108 20-21 4.341717367953603 1108 22-23 3.6998720467046553 1108 24-25 2.5461961153646655 1108 26-27 4.279843254623952 1108 28-29 5.752897744433792 1108 30-31 4.140982188924831 1108 32-33 4.123512514008635 1108 34-35 2.6304697387281486 1108 36-37 0.7621327275769758 1108 38-39 2.0544104536878542 1108 40-41 5.006540741596307 1108 42-43 2.769297409080174 1108 44-45 3.317239940832941 1108 46-47 4.347519760606019 1108 48-49 4.422090918527221 1108 50-51 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36-37 -23.75259978271109 2213 38-39 -11.521111489809375 2213 40-41 -17.05297953630339 2213 42-43 -23.264365142360155 2213 44-45 -17.13925566446546 2213 46-47 -16.938160452170315 2213 48-49 -6.13456524437122 2213 50-51 -23.44498957572937 2213 52-53 -17.83463878180831 2213 54-55 -17.758199293216272 2213 56-57 -17.556960596704297 2213 58-59 -18.019095518587406 2213 60-61 -19.46996554762575 2213 62-63 -17.49876392201736 2213 64-65 -17.62115660503697 2213 66-67 -8.532456070140718 2213 68-69 -22.87226106928208 2213 70-71 -17.61441159113901 2213 72-73 -9.455650171743365 2213 74-75 -4.437076638302759 2213 76-77 -3.2758063052846182 2213 78-79 -4.771400434955144 2213 80-81 -3.308333215202186 2213 82-83 -3.321534534084897 2213 84-85 -3.0679240160043157 2213 86-87 -3.221112316506712 2213 88-89 -2.572321562657269 2213 90-91 -2.718269773445737 2213 92-93 -3.7376813911440117 2213 94-95 -3.6239006458671916 2213 96-97 -3.2721636579883935 2213 98-99 -3.538594054700635 2213 100 -3.2169558496572037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 749.0 3 630.0 4 2713.0 5 10785.0 6 18159.0 7 20864.0 8 18344.0 9 18739.0 10 22743.0 11 29152.0 12 38355.0 13 50857.0 14 42383.0 15 47982.0 16 54601.0 17 57267.0 18 57926.0 19 57215.0 20 57614.0 21 56500.0 22 59115.0 23 64487.0 24 70725.0 25 77628.0 26 84158.0 27 91027.0 28 97239.0 29 102346.0 30 108298.0 31 118265.0 32 134209.0 33 159674.0 34 190352.0 35 217007.0 36 230298.0 37 191719.0 38 57616.0 39 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.95118142839759 21.91754734484813 14.45616048018889 23.675110746565394 2 14.364079564307177 21.658003048927014 41.27302314762305 22.704894239142764 3 16.93256931783571 27.555514755506355 30.39439428069751 25.117521645960426 4 11.45945232621561 18.254273791321545 41.26750019818486 29.01877368427798 5 12.576518362949516 37.93365958401996 36.58030643576747 12.90951561726305 6 28.873925118417187 39.45742735955008 19.60061432766215 12.068033194370575 7 24.914889110455597 32.94708401568417 25.27806334626571 16.859963527594527 8 22.80940301652576 37.03601121299583 22.679936336236622 17.474649434241783 9 23.92471291703472 17.322885716580082 22.93274012119565 35.81966124518954 10-11 22.532081547757233 27.753774022926482 30.129420281216472 19.584724148099813 12-13 23.412013808117134 25.39470012928888 30.092675955350728 21.10061010724326 14-15 20.76940416970958 26.916307022493353 27.300547616310038 25.01374119148703 16-17 20.556847439152612 30.55489889843157 27.99081713093699 20.897436531478824 18-19 20.397198076197455 29.647045303180896 29.523689427165905 20.432067193455744 20-21 21.90910652676113 29.074337087475794 28.337145268371312 20.679411117391766 22-23 20.86967433663951 29.141060922937516 29.096917380394956 20.892347360028023 24-25 21.209066560255447 28.880949681490648 28.584082470754137 21.325901287499764 26-27 21.17787944356833 28.967249379812465 28.605874072743266 21.248997103875944 28-29 20.94275445063074 28.858584295349242 29.028606088661522 21.17005516535849 30-31 21.518645931358016 29.215842681100867 28.332628941464368 20.932882446076746 32-33 20.80150870536645 29.13965601565535 28.928878653658852 21.129956625319345 34-35 21.010562717573713 28.567835208311248 28.702747872028063 21.718854202086977 36-37 21.397511374729515 29.200961704439077 28.330887440008844 21.070639480822567 38-39 21.071865285336596 28.96202793392066 28.62020004773036 21.345906733012384 40-41 21.430039694775235 28.883454981172463 28.126087558666164 21.560417765386138 42-43 20.93432917480155 29.071778087455215 28.83369241954121 21.160200318202023 44-45 21.413182300879434 28.576962585856947 28.601897679554604 21.40795743370901 46-47 21.42635506269879 29.037269638808226 28.526907443093734 21.00946785539925 48-49 21.226965421634013 28.73366819226505 28.674799717553327 21.36456666854761 50-51 21.550162197885527 28.775143313438388 28.36470372107246 21.30999076760362 52-53 21.03536373943306 28.841526337177818 28.726771771257305 21.396338152131822 54-55 21.520935303363384 28.689734113637442 28.39976437795453 21.389566205044645 56-57 21.473083059738947 28.800402410078092 28.409294258140992 21.317220272041975 58-59 21.20204240860948 28.379920852674033 28.713795117321766 21.704241621394722 60-61 21.635247430541476 28.74051571305457 28.233821584864394 21.39041527153956 62-63 21.205020029212307 28.892799698590537 28.625579285528424 21.276600986668733 64-65 21.350543395373634 28.30332993329544 28.5863893228195 21.75973734851143 66-67 21.357987312138814 28.647854002446216 28.3918128435294 21.60234584188557 68-69 21.697281413560084 28.522392214192966 28.375024288238944 21.405302084008007 70-71 21.865228097216217 28.515798575214752 28.151369335162872 21.467603992406158 72-73 21.36526044860359 28.750596259566034 28.61441495860182 21.269728333228556 74-75 21.608998250706403 28.35755664833421 28.256888005579366 21.77655709538002 76-77 21.671375791101333 28.752872553524117 28.185530464574594 21.390221190799956 78-79 21.59214706729951 28.462118448800005 28.41258750793065 21.533146975969835 80-81 21.844316440508628 28.36430367839473 28.148999177652517 21.642380703444122 82-83 21.347858928958 28.589113655088276 28.717335141224186 21.345692274729533 84-85 21.503806947271897 28.180765593045848 28.33936747108403 21.976059988598223 86-87 21.664206789261637 28.595779352690275 28.32489735736774 21.415116500680345 88-89 21.450561447806148 28.20154496748636 28.566362904781816 21.78153067992568 90-91 21.728619442263568 28.192564559832533 28.30072782325584 21.778088174648058 92-93 21.361979419881322 28.518876117619342 28.562843791507987 21.556300670991355 94-95 21.39306516806671 27.978825030951317 28.461645225753784 22.166464575228193 96-97 21.435266114147797 28.296386591656606 28.529369572419018 21.73897772177658 98-99 21.276023038314428 28.10661797307763 28.605593013425718 22.011765975182232 100 21.685144369746038 27.872867495855903 28.244488992752903 22.19749914164516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 276.0 1 329.0 2 309.0 3 247.5 4 289.0 5 374.0 6 535.5 7 790.5 8 1177.0 9 1713.5 10 2424.0 11 3300.0 12 4232.5 13 5042.5 14 5806.5 15 6463.5 16 7117.5 17 7927.5 18 8641.5 19 9282.0 20 10141.5 21 11108.0 22 12228.5 23 13842.0 24 15949.5 25 18662.5 26 22309.0 27 27335.0 28 33883.0 29 42133.5 30 51495.0 31 61514.0 32 71674.5 33 81355.5 34 90286.5 35 97841.5 36 104029.5 37 109300.0 38 113146.5 39 115199.5 40 115326.0 41 113593.0 42 110357.5 43 106258.0 44 101394.5 45 95297.0 46 89493.5 47 83615.5 48 77770.5 49 73025.0 50 68655.5 51 63924.0 52 58153.0 53 52704.0 54 47996.0 55 43491.0 56 39205.0 57 35094.0 58 31171.0 59 27143.5 60 23112.0 61 19436.0 62 15994.5 63 12682.5 64 10045.0 65 7958.5 66 6255.0 67 4857.0 68 3691.0 69 2815.0 70 2081.0 71 1512.5 72 1139.0 73 847.5 74 623.0 75 451.5 76 341.5 77 245.0 78 160.0 79 110.5 80 68.5 81 50.0 82 37.5 83 25.5 84 22.0 85 19.5 86 14.0 87 5.5 88 4.0 89 5.0 90 4.0 91 2.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 6.875320564001315 2 23.442436087724708 3 5.410973167919242 4 22.489960873714892 5 11.650500797316719 6 8.89164513629443 7 7.41180764614591 8 7.80766969379653 9 4.697582588692747 10-11 6.174035070161664 12-13 9.274555846388306 14-15 6.718708722210734 16-17 7.003049451297453 18-19 6.19353566364199 20-21 7.049280575274866 22-23 5.14788072701156 24-25 4.752496995804797 26-27 8.763217171118812 28-29 5.73396554502687 30-31 3.7578931411629566 32-33 7.805443682653965 34-35 6.456554517404464 36-37 6.319719220888742 38-39 5.33801151344474 40-41 7.280969701964703 42-43 5.715623949083583 44-45 5.63705127478691 46-47 5.078672016942704 48-49 3.497964947499251 50-51 5.96760473106474 52-53 3.89163777757991 54-55 4.339415556175323 56-57 4.287904554529178 58-59 3.231726656133893 60-61 3.639951344179588 62-63 2.928566014660031 64-65 4.103311200772959 66-67 2.0528605898892733 68-69 4.563065287251101 70-71 2.127257193695348 72-73 1.3840086303003902 74-75 0.6593040274980444 76-77 0.9409956193572457 78-79 1.4993932739877536 80-81 1.7462965429495052 82-83 0.6567836677746438 84-85 1.0923459802653994 86-87 0.38316826510210583 88-89 0.6481187814263102 90-91 0.29705290842153365 92-93 0.29142349181306215 94-95 0.456516252088955 96-97 0.48427700261896645 98-99 0.42010901199691225 100 0.3383536936699602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2717866.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.51159502114794 #Duplication Level Percentage of deduplicated Percentage of total 1 97.44206293748327 94.04288916247593 2 2.117304421412832 4.086888537117623 3 0.2722967214195505 0.7883937270968999 4 0.0769838870959283 0.2971935093822403 5 0.034921353760153366 0.16851577758450814 6 0.016799625381597884 0.09728151848014638 7 0.00955518206134398 0.06455301030204375 8 0.007925191373360684 0.06118982882327053 9 0.003984103005025693 0.034606092216922034 >10 0.01745163187683767 0.2447789929004459 >50 3.3168192635700864E-4 0.023575000885719146 >100 3.4506939749601607E-4 0.06978732144705348 >500 3.81938062460013E-5 0.020347521287231083 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6793572604388884E-5 0.0 5 0.0 0.0 0.0 3.6793572604388884E-5 0.0 6 0.0 0.0 0.0 3.6793572604388884E-5 0.0 7 0.0 0.0 0.0 3.6793572604388884E-5 0.0 8 7.358714520877777E-5 0.0 0.0 3.6793572604388884E-5 0.0 9 7.358714520877777E-5 0.0 0.0 3.6793572604388884E-5 0.0 10-11 7.358714520877777E-5 0.0 0.0 3.6793572604388884E-5 0.0 12-13 7.358714520877777E-5 0.0 0.0 7.358714520877777E-5 0.0 14-15 7.358714520877777E-5 0.0 0.0 7.358714520877777E-5 0.0 16-17 7.358714520877777E-5 0.0 0.0 9.198393151097222E-5 0.0 18-19 7.358714520877777E-5 0.0 0.0 1.1038071781316665E-4 0.0 20-21 7.358714520877777E-5 0.0 0.0 1.1038071781316665E-4 0.0 22-23 7.358714520877777E-5 0.0 0.0 1.1038071781316665E-4 0.0 24-25 7.358714520877777E-5 0.0 0.0 1.4717429041755553E-4 3.6793572604388884E-5 26-27 7.358714520877777E-5 0.0 0.0 2.5755500823072217E-4 3.6793572604388884E-5 28-29 7.358714520877777E-5 0.0 0.0 4.415228712526666E-4 3.6793572604388884E-5 30-31 7.358714520877777E-5 0.0 0.0 0.001122203964433861 3.6793572604388884E-5 32-33 9.198393151097222E-5 0.0 0.0 0.0033114215343949998 3.6793572604388884E-5 34-35 1.1038071781316665E-4 0.0 0.0 0.007671459888015082 3.6793572604388884E-5 36-37 1.1038071781316665E-4 0.0 0.0 0.014091938307480942 3.6793572604388884E-5 38-39 1.1038071781316665E-4 0.0 0.0 0.024890851866869082 3.6793572604388884E-5 40-41 1.1038071781316665E-4 0.0 0.0 0.03786058620991616 3.6793572604388884E-5 42-43 1.1038071781316665E-4 0.0 0.0 0.053479457780479245 3.6793572604388884E-5 44-45 1.1038071781316665E-4 0.0 0.0 0.0727960833977834 3.6793572604388884E-5 46-47 1.1038071781316665E-4 0.0 0.0 0.09673030237693837 3.6793572604388884E-5 48-49 1.1038071781316665E-4 0.0 0.0 0.12289053249865887 3.6793572604388884E-5 50-51 1.1038071781316665E-4 0.0 0.0 0.15234378736847218 3.6793572604388884E-5 52-53 1.1038071781316665E-4 3.6793572604388884E-5 0.0 0.18409664052605978 3.6793572604388884E-5 54-55 1.1038071781316665E-4 3.6793572604388884E-5 0.0 0.2187561859193941 3.6793572604388884E-5 56-57 1.1038071781316665E-4 3.6793572604388884E-5 0.0 0.26071925547469965 3.6793572604388884E-5 58-59 1.1038071781316665E-4 3.6793572604388884E-5 0.0 0.30890043880014684 3.6793572604388884E-5 60-61 1.2877750411536109E-4 3.6793572604388884E-5 0.0 0.3603746468736869 3.6793572604388884E-5 62-63 1.4717429041755553E-4 3.6793572604388884E-5 0.0 0.41365174000484206 3.6793572604388884E-5 64-65 1.4717429041755553E-4 3.6793572604388884E-5 0.0 0.4755569259117263 3.6793572604388884E-5 66-67 1.4717429041755553E-4 3.6793572604388884E-5 0.0 0.5459430303039222 3.6793572604388884E-5 68-69 1.4717429041755553E-4 3.6793572604388884E-5 0.0 0.6259322571458636 3.6793572604388884E-5 70-71 1.4717429041755553E-4 3.6793572604388884E-5 0.0 0.7203077708761212 3.6793572604388884E-5 72-73 1.4717429041755553E-4 3.6793572604388884E-5 0.0 0.8222075702039762 3.6793572604388884E-5 74-75 1.4717429041755553E-4 3.6793572604388884E-5 0.0 0.9311533386855717 3.6793572604388884E-5 76-77 1.4717429041755553E-4 3.6793572604388884E-5 0.0 1.0531792222280274 3.6793572604388884E-5 78-79 1.4717429041755553E-4 3.6793572604388884E-5 0.0 1.1939514310124193 3.6793572604388884E-5 80-81 1.4717429041755553E-4 3.6793572604388884E-5 0.0 1.3557879601128238 3.6793572604388884E-5 82-83 1.4717429041755553E-4 3.6793572604388884E-5 0.0 1.5277979120383418 3.6793572604388884E-5 84-85 1.4717429041755553E-4 3.6793572604388884E-5 0.0 1.7056212484353535 3.6793572604388884E-5 86-87 1.4717429041755553E-4 3.6793572604388884E-5 0.0 1.8982723945919335 3.6793572604388884E-5 88 1.4717429041755553E-4 3.6793572604388884E-5 0.0 2.0634203452267332 3.6793572604388884E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4620 0.0 22.214151 1 GTATCAA 2860 0.0 22.000551 1 TACATGG 4805 0.0 21.101255 2 ATCAACG 2630 0.0 20.055107 3 TCAACGC 2640 0.0 19.767885 4 ACATGGG 5030 0.0 19.095686 3 GTACTAA 775 0.0 18.917858 1 CAACGCA 2590 0.0 18.524797 5 TATCAAC 3175 0.0 17.890926 2 AACGCAG 2745 0.0 17.160608 6 GTATTAG 980 0.0 16.83062 1 CGCAGAG 2780 0.0 16.775564 8 ACGCAGA 2845 0.0 16.494883 7 GTATAGG 745 0.0 15.579722 1 CATGGGG 2910 0.0 14.499628 4 GTCTTAC 845 0.0 14.458914 1 GTGTACG 255 0.004985013 14.373863 1 ATTATAC 1435 0.0 14.3155155 3 GTGTATA 1425 0.0 14.146907 1 TAACGGT 275 9.487141E-4 14.131935 4 >>END_MODULE