FastQCFastQC Report
Wed 25 May 2016
SRR1295115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717866
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42050.027.462231
TCAACGC45850.025.7445894
CAACGCA45350.025.7173355
ATCAACG46000.025.4552333
AACGCAG46800.024.98746
TATCAAC52550.021.7728712
ACGCAGA54200.021.3135347
CGCAGAG54700.021.2045578
GTACATG61500.020.6226271
TACATGG63600.018.9205232
GCACCGT2803.97813E-818.4502476
ACATGGG63700.018.1606433
CTATGCG1851.6622322E-417.7676479
TCCGTAC2654.456124E-717.745563
CATGGGG33550.017.521414
GCAGAGT69950.016.782479
GGTATCA30350.016.0976491
CTAATAC13400.015.7922233
GTATATA20850.015.38485051
AGAGTAC60450.014.83681510-11
Produced by FastQC (version 0.11.5)