FastQCFastQC Report
Wed 25 May 2016
SRR1295114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740478
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT9150.017.9852121
GTACATG101850.017.0551971
GTATAGA11150.016.9457071
TATACTG13200.016.6143935
CTGTCGC6350.016.5757459
GTATATA18900.016.124311
TACCGTG3101.2492419E-616.0798157
TACATGG104000.016.0537682
GTACTAA8200.015.6091181
ATAATAC18150.015.5140933
GTATAGG8300.015.4210561
CTGTGCG4952.5283953E-1015.1884539
GTATAGC6103.092282E-1014.9876781
GTCTATA9050.014.8165461
GTATTAT15950.014.5209771
ACATGGG105750.014.148873
GTACTCG3959.591258E-713.9445526
GTATTAG11100.013.7274511
ACTGTCG3002.6053103E-413.3624048
TGTACCG2250.00837270513.2915155
Produced by FastQC (version 0.11.5)