FastQCFastQC Report
Wed 25 May 2016
SRR1295114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740478
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70790.2583126009404199No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52350.19102506934921573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT1552.9442837E-522.2540741
GTACCGT2001.41774035E-518.7859066
GTATATA23450.017.2311481
TGTACCG2556.051163E-616.6009545
ACCGTCC2355.6660305E-515.9865468
GAGTACT28800.015.89807812-13
AGTACCG2457.978517E-515.3587065
TATACCG1850.003357233715.2549315
TAGCGTG2206.1454973E-414.9660125
CTAGTAC6350.014.8139593
CTAGCGT1950.004764203414.4726294
GTCCTAG11450.014.2020711
TACCGAA2000.005696037314.0881447
GTACATG126200.013.9396561
GTATCAA105000.013.7975271
TACACTG21700.013.6556255
GTACTAT10850.013.6249441
TACATGG127450.013.5626442
ACATGGG121100.013.5548953
CATGGGG36200.013.3831834
Produced by FastQC (version 0.11.5)