##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295114_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2740478 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.502191588474712 33.0 33.0 33.0 27.0 33.0 2 30.36041340233346 33.0 33.0 33.0 27.0 33.0 3 31.082845036522826 33.0 33.0 33.0 27.0 33.0 4 35.27457545727424 37.0 37.0 37.0 33.0 37.0 5 35.56243728283898 37.0 37.0 37.0 33.0 37.0 6 35.36357671909791 37.0 37.0 37.0 33.0 37.0 7 35.25862750950746 37.0 37.0 37.0 33.0 37.0 8 35.2565037924041 37.0 37.0 37.0 33.0 37.0 9 35.21080373569866 37.0 37.0 37.0 33.0 37.0 10-11 35.199103039688694 37.0 37.0 37.0 33.0 37.0 12-13 35.123604714214096 37.0 37.0 37.0 33.0 37.0 14-15 36.62735971607873 40.0 37.0 40.0 33.0 40.0 16-17 36.62191376832801 40.0 37.0 40.0 33.0 40.0 18-19 36.63542236062468 40.0 37.0 40.0 33.0 40.0 20-21 36.630410643690624 40.0 37.0 40.0 33.0 40.0 22-23 36.59569498459758 40.0 37.0 40.0 33.0 40.0 24-25 36.574429351375926 40.0 37.0 40.0 33.0 40.0 26-27 36.55456201436392 40.0 37.0 40.0 33.0 40.0 28-29 36.50152436910641 40.0 37.0 40.0 33.0 40.0 30-31 36.40975187540276 40.0 37.0 40.0 33.0 40.0 32-33 36.374951194645604 40.0 37.0 40.0 33.0 40.0 34-35 36.292939224471056 37.0 37.0 40.0 33.0 40.0 36-37 36.28139306354585 37.0 37.0 40.0 33.0 40.0 38-39 36.21081395289435 37.0 37.0 40.0 30.0 40.0 40-41 36.153503695340746 37.0 37.0 40.0 27.0 40.0 42-43 36.07046325495041 37.0 37.0 40.0 27.0 40.0 44-45 35.98495791610077 37.0 37.0 40.0 27.0 40.0 46-47 35.888523644415315 37.0 37.0 40.0 27.0 40.0 48-49 35.78125987510208 37.0 37.0 40.0 27.0 40.0 50-51 35.68731604486516 37.0 37.0 40.0 27.0 40.0 52-53 35.596418763441996 37.0 37.0 40.0 27.0 40.0 54-55 35.482154025684565 37.0 37.0 40.0 27.0 40.0 56-57 35.3609490753073 37.0 33.0 40.0 27.0 40.0 58-59 35.23531095670171 37.0 33.0 40.0 27.0 40.0 60-61 35.073306736999896 37.0 33.0 40.0 27.0 40.0 62-63 34.893585899978035 37.0 33.0 40.0 27.0 40.0 64-65 34.71755182854962 37.0 33.0 40.0 27.0 40.0 66-67 34.5001572718336 37.0 33.0 40.0 27.0 40.0 68-69 34.28687805558009 37.0 33.0 38.5 27.0 40.0 70-71 34.053184152545654 37.0 33.0 37.0 27.0 40.0 72-73 33.82940512567515 37.0 33.0 37.0 27.0 40.0 74-75 33.58990657834144 37.0 33.0 37.0 27.0 40.0 76-77 33.35023488603083 37.0 33.0 37.0 27.0 38.5 78-79 33.11683600452184 37.0 33.0 37.0 24.5 37.0 80-81 32.901722801642634 37.0 33.0 37.0 22.0 37.0 82-83 32.69004148181449 37.0 33.0 37.0 22.0 37.0 84-85 32.50516898876765 37.0 33.0 37.0 22.0 37.0 86-87 32.53128377604199 37.0 33.0 37.0 22.0 37.0 88-89 32.5366684206186 37.0 33.0 37.0 22.0 37.0 90-91 32.547492627198615 37.0 33.0 37.0 22.0 37.0 92-93 32.514085316503184 33.0 33.0 37.0 22.0 37.0 94-95 32.537914188692625 33.0 33.0 37.0 22.0 37.0 96-97 32.49664511081643 33.0 33.0 37.0 22.0 37.0 98-99 32.42103622068851 33.0 33.0 37.0 22.0 37.0 100 31.889051472042468 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5876224546172111 1101 2 4.115622606627561 1101 3 2.3161189160458946 1101 4 1.069584760350601 1101 5 0.4793344298255775 1101 6 0.4494400888222927 1101 7 0.469963916899637 1101 8 0.46339398915975494 1101 9 0.49656549247215764 1101 10-11 0.5584679117359954 1101 12-13 0.5802010313953971 1101 14-15 0.8975299823371046 1101 16-17 0.8210169184769285 1101 18-19 0.8797148500221041 1101 20-21 0.8809602550904145 1101 22-23 0.843204247592432 1101 24-25 0.8090508625462505 1101 26-27 0.858188925049518 1101 28-29 0.725400442213413 1101 30-31 0.6870225703231796 1101 32-33 0.9166022757225534 1101 34-35 0.9687138741569754 1101 36-37 0.9016282427412321 1101 38-39 0.9360289081573541 1101 40-41 1.0006241092160124 1101 42-43 0.9619580970822028 1101 44-45 0.9425561626703285 1101 46-47 0.8774631650704237 1101 48-49 0.8765768160156995 1101 50-51 0.8698156555980177 1101 52-53 0.8158685698214256 1101 54-55 0.9518919203844192 1101 56-57 0.8443496067714094 1101 58-59 0.8272335045479977 1101 60-61 0.9049977304536654 1101 62-63 0.9044010572399515 1101 64-65 0.8734237734733981 1101 66-67 0.8901777939181912 1101 68-69 0.8535752091459798 1101 70-71 0.8195163308190416 1101 72-73 0.6864317076171815 1101 74-75 0.7911266391212592 1101 76-77 0.7994731192625153 1101 78-79 0.7811523564167402 1101 80-81 0.6995769234538614 1101 82-83 0.7886408478453362 1101 84-85 0.8849509508080828 1101 86-87 0.8437334246104626 1101 88-89 0.7756396174664957 1101 90-91 0.7556404083563564 1101 92-93 0.8205348624762507 1101 94-95 0.7870062493366703 1101 96-97 0.7035968815340752 1101 98-99 0.7267221768791501 1101 100 0.8356633749552245 1102 1 0.4004098498550661 1102 2 3.9215481223025037 1102 3 2.1639773281817973 1102 4 0.9198288360736271 1102 5 0.36975063405289177 1102 6 0.33791490294214555 1102 7 0.3449020207723521 1102 8 0.3209271663650668 1102 9 0.4689397880068995 1102 10-11 0.4497807740751725 1102 12-13 0.4676626511319171 1102 14-15 0.7418629455116488 1102 16-17 0.6933590129952307 1102 18-19 0.7976401706147627 1102 20-21 0.6941275787863503 1102 22-23 0.7133172522732707 1102 24-25 0.7132230631957412 1102 26-27 0.7019952334219539 1102 28-29 0.686754583840461 1102 30-31 0.7556272686137007 1102 32-33 0.872019131433504 1102 34-35 0.8765120968764819 1102 36-37 0.8026715598201193 1102 38-39 0.7607963979531149 1102 40-41 0.6896349020762429 1102 42-43 0.853608544035211 1102 44-45 0.8754661249381712 1102 46-47 0.7958172365217493 1102 48-49 0.747177448675231 1102 50-51 0.6787199078217938 1102 52-53 0.76556511942524 1102 54-55 0.6739469267484282 1102 56-57 0.6438982535253785 1102 58-59 0.7375960694385171 1102 60-61 0.6535756095940712 1102 62-63 0.7452684073994931 1102 64-65 0.7069292809200647 1102 66-67 0.7042387437616213 1102 68-69 0.655916269168408 1102 70-71 0.6642651654822416 1102 72-73 0.7404415660104533 1102 74-75 0.725437913125738 1102 76-77 0.7064549920545531 1102 78-79 0.7723737311746888 1102 80-81 0.7836119880958563 1102 82-83 0.7822506849267441 1102 84-85 0.6606587175398317 1102 86-87 0.7360494525277517 1102 88-89 0.6445509795171418 1102 90-91 0.7324365259078505 1102 92-93 0.7739047794398175 1102 94-95 0.7649774215231133 1102 96-97 0.6869605967052266 1102 98-99 0.7429259532866439 1102 100 0.5886343631506747 1103 1 0.46979390901443807 1103 2 3.9646376897246505 1103 3 2.215485686990956 1103 4 1.0208578224973337 1103 5 0.4316519236707492 1103 6 0.42005604799391705 1103 7 0.3491762135378025 1103 8 0.34215232038110344 1103 9 0.39470409564382436 1103 10-11 0.5057382344497725 1103 12-13 0.4838043377554868 1103 14-15 0.6370668732716354 1103 16-17 0.7139376394337518 1103 18-19 0.7721835933118157 1103 20-21 0.7229196375175064 1103 22-23 0.7266334244626691 1103 24-25 0.696650952936551 1103 26-27 0.6460902902935572 1103 28-29 0.6227642807045655 1103 30-31 0.6597357362095195 1103 32-33 0.6982226265132994 1103 34-35 0.7877429421194506 1103 36-37 0.7241411820764156 1103 38-39 0.7428801190761689 1103 40-41 0.6573764295868614 1103 42-43 0.7019600299072479 1103 44-45 0.752282640464152 1103 46-47 0.7380950628561536 1103 48-49 0.6119862100685083 1103 50-51 0.6942701817694896 1103 52-53 0.6369376182307107 1103 54-55 0.5951975063010977 1103 56-57 0.5395054607335368 1103 58-59 0.6765075835856322 1103 60-61 0.7031983854171884 1103 62-63 0.7083316230502845 1103 64-65 0.6399810485196369 1103 66-67 0.6218666697646569 1103 68-69 0.6251145303660692 1103 70-71 0.6613363876843721 1103 72-73 0.7048989485865818 1103 74-75 0.5902892603249583 1103 76-77 0.5425229262546267 1103 78-79 0.661235614359434 1103 80-81 0.6108892201181817 1103 82-83 0.605360556043685 1103 84-85 0.6688919339961643 1103 86-87 0.7280710030971491 1103 88-89 0.6135940242422109 1103 90-91 0.6552530456320511 1103 92-93 0.7360769074365408 1103 94-95 0.5501026298275349 1103 96-97 0.6134241853163758 1103 98-99 0.6711947418792121 1103 100 0.6700352813562773 1104 1 0.3433792603690904 1104 2 3.5162688355086615 1104 3 2.0686161554368248 1104 4 0.8223341712749104 1104 5 0.26943141001680715 1104 6 0.22772670669917972 1104 7 0.13954775334956793 1104 8 0.22779037614797915 1104 9 0.17315046174765314 1104 10-11 0.20483437003186822 1104 12-13 0.28364472190258994 1104 14-15 0.4496226274602577 1104 16-17 0.5308754123329891 1104 18-19 0.4466336508950022 1104 20-21 0.39277317698048364 1104 22-23 0.37185824457255734 1104 24-25 0.3607192579930256 1104 26-27 0.3395063152122546 1104 28-29 0.33177707588928484 1104 30-31 0.35135858331899783 1104 32-33 0.48405496143204374 1104 34-35 0.46725342290804406 1104 36-37 0.40813543077640446 1104 38-39 0.3574982720622586 1104 40-41 0.4000892538736309 1104 42-43 0.4813104359051863 1104 44-45 0.5214137579020104 1104 46-47 0.45608458611700087 1104 48-49 0.3508820085203155 1104 50-51 0.3279600602481736 1104 52-53 0.28898977191763464 1104 54-55 0.3663073068899436 1104 56-57 0.40250602009767533 1104 58-59 0.38632701587422247 1104 60-61 0.3416676147203148 1104 62-63 0.3555333175564783 1104 64-65 0.3836744743172247 1104 66-67 0.3209290059306227 1104 68-69 0.42813671649718543 1104 70-71 0.36492659028109387 1104 72-73 0.32709207175522437 1104 74-75 0.30239167849499893 1104 76-77 0.3175197811811685 1104 78-79 0.31264425631940895 1104 80-81 0.3424765697418195 1104 82-83 0.4356583715539415 1104 84-85 0.426011637921917 1104 86-87 0.4861184362042721 1104 88-89 0.33286194562671056 1104 90-91 0.31899779956852825 1104 92-93 0.4679489604487088 1104 94-95 0.36791350481666996 1104 96-97 0.36060104319091124 1104 98-99 0.33638698550713286 1104 100 0.346659205619666 1105 1 0.40696046495359184 1105 2 3.7389454305496237 1105 3 2.145991249121959 1105 4 1.0318420277098852 1105 5 0.35044755856292653 1105 6 0.2901618840197102 1105 7 0.34738348659989526 1105 8 0.24732197070419204 1105 9 0.31315643726605913 1105 10-11 0.2790640096306092 1105 12-13 0.3191697499466528 1105 14-15 0.4097438571425869 1105 16-17 0.40245833011075405 1105 18-19 0.4214328419975928 1105 20-21 0.3887787773758049 1105 22-23 0.3649887073981617 1105 24-25 0.36953574355969465 1105 26-27 0.4098797124272693 1105 28-29 0.36477267365808785 1105 30-31 0.2650199114405396 1105 32-33 0.41284503809290385 1105 34-35 0.5605673675950982 1105 36-37 0.43283289214840437 1105 38-39 0.4199640401677698 1105 40-41 0.314863818779493 1105 42-43 0.4069212569602172 1105 44-45 0.4570805036665959 1105 46-47 0.3797232920866307 1105 48-49 0.35811390782031793 1105 50-51 0.33144018216754745 1105 52-53 0.18835018814686322 1105 54-55 0.3117334421264104 1105 56-57 0.31321722975267363 1105 58-59 0.3732050310271475 1105 60-61 0.38994404054054144 1105 62-63 0.36869404982034837 1105 64-65 0.27276140100438084 1105 66-67 0.3316965523622173 1105 68-69 0.3843527366795101 1105 70-71 0.2526284471075897 1105 72-73 0.2213628590338672 1105 74-75 0.2530223459878371 1105 76-77 0.3127686046314295 1105 78-79 0.31395008252091117 1105 80-81 0.18496653066321755 1105 82-83 0.18993470097016285 1105 84-85 0.27305731680890233 1105 86-87 0.3228451424213574 1105 88-89 0.3021980788662333 1105 90-91 0.3454115966219078 1105 92-93 0.3380470843195553 1105 94-95 0.2766575316209128 1105 96-97 0.29211266134970515 1105 98-99 0.3550154482774275 1105 100 0.3067458686713671 1106 1 0.43928676448033954 1106 2 3.8501623565649865 1106 3 2.136957855522418 1106 4 0.9714462857551638 1106 5 0.33929980196549536 1106 6 0.24924344501217632 1106 7 0.28884499424471954 1106 8 0.29172991434172246 1106 9 0.3002529688882447 1106 10-11 0.2399843464703295 1106 12-13 0.27729873265435145 1106 14-15 0.3365777066558877 1106 16-17 0.29149493667831905 1106 18-19 0.3210103518942091 1106 20-21 0.22915328238232746 1106 22-23 0.3045112984371414 1106 24-25 0.2687132329697022 1106 26-27 0.21130987925637612 1106 28-29 0.20541327122890607 1106 30-31 0.20516281093312472 1106 32-33 0.2676752407084635 1106 34-35 0.45294218934101593 1106 36-37 0.2328255243493942 1106 38-39 0.25586747664377896 1106 40-41 0.2613021444422756 1106 42-43 0.2666551557151564 1106 44-45 0.3356814747403405 1106 46-47 0.2950750941808238 1106 48-49 0.15671325016558768 1106 50-51 0.14520783151846928 1106 52-53 0.08713586310561539 1106 54-55 0.11450532964367 1106 56-57 0.10513770027615976 1106 58-59 0.06154916757210316 1106 60-61 0.08390913496881325 1106 62-63 0.20705171479448836 1106 64-65 0.09443969555531595 1106 66-67 -0.008077297984442566 1106 68-69 0.00886084928812636 1106 70-71 0.008950132590896942 1106 72-73 0.0753922746729998 1106 74-75 -0.023516162209723745 1106 76-77 -0.018665580849166474 1106 78-79 0.08375648139466563 1106 80-81 0.07892327256824672 1106 82-83 0.12156436099451895 1106 84-85 0.09378605777901328 1106 86-87 0.2724356707522304 1106 88-89 0.22527104134427844 1106 90-91 0.11728754404598618 1106 92-93 0.2538767082536424 1106 94-95 0.25836314562333484 1106 96-97 0.11110893871229166 1106 98-99 0.2192236030311534 1106 100 0.15803582487473733 1107 1 0.44262513185972097 1107 2 3.5739636394037078 1107 3 2.1109141942053284 1107 4 0.9298043075918443 1107 5 0.2835064941155139 1107 6 0.16462841705997278 1107 7 0.11505328572802398 1107 8 0.07001902514613789 1107 9 0.13148657511985817 1107 10-11 0.048509346891833616 1107 12-13 0.10186945377542145 1107 14-15 0.12269096546607727 1107 16-17 0.022130117675153826 1107 18-19 0.11552116425435344 1107 20-21 0.19479245569080206 1107 22-23 0.18820902805828155 1107 24-25 0.08700148268728469 1107 26-27 0.1733275200952562 1107 28-29 0.11000048547452934 1107 30-31 0.04754026563273328 1107 32-33 0.20126693019693676 1107 34-35 0.3310517364454739 1107 36-37 0.17905456621868865 1107 38-39 0.11695490284924404 1107 40-41 0.12053612642088041 1107 42-43 0.2031770995795199 1107 44-45 0.16586973361073376 1107 46-47 0.007881562809558318 1107 48-49 0.02046372286010012 1107 50-51 -0.0034687442704139926 1107 52-53 -0.07025665760262001 1107 54-55 -0.04455185856462407 1107 56-57 0.0065495815882883335 1107 58-59 0.05414532628184077 1107 60-61 0.07852192289096394 1107 62-63 0.16478544868373746 1107 64-65 0.09834680174960653 1107 66-67 0.06158989496886136 1107 68-69 0.02491846262039843 1107 70-71 0.030457130878353667 1107 72-73 0.0476887631248033 1107 74-75 0.028601268355359366 1107 76-77 0.04407859447051976 1107 78-79 0.09053633581680032 1107 80-81 0.2047125519770674 1107 82-83 0.16073643504557822 1107 84-85 0.12105375702727628 1107 86-87 0.0915273506061709 1107 88-89 0.10092709315882331 1107 90-91 0.06423149873474898 1107 92-93 0.17508485521888417 1107 94-95 -0.018421471873466544 1107 96-97 0.06857079798541577 1107 98-99 0.12658210177745843 1107 100 0.12862368824735526 1108 1 0.4703077225503165 1108 2 3.6261432766099126 1108 3 2.1649359666584296 1108 4 0.9921214981421258 1108 5 0.29599102427387436 1108 6 0.2028600061381738 1108 7 0.08714042813156908 1108 8 0.09172018000805338 1108 9 0.10730046119828529 1108 10-11 0.11571524783987286 1108 12-13 0.13045176431258199 1108 14-15 0.07772640430451361 1108 16-17 -0.02097122453018585 1108 18-19 0.08448905733690282 1108 20-21 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52-53 0.1104590788124824 2210 54-55 0.03156977466388611 2210 56-57 -0.03327924041559527 2210 58-59 0.12693675584412034 2210 60-61 0.08836432498369362 2210 62-63 0.21486516123889032 2210 64-65 -0.14291653184232445 2210 66-67 -0.11432204861436901 2210 68-69 0.09855838268556738 2210 70-71 0.18244735640229948 2210 72-73 0.1799895780690619 2210 74-75 -0.5766568867720636 2210 76-77 -0.053647868481895955 2210 78-79 0.1055707839682114 2210 80-81 0.09969625082534606 2210 82-83 0.13233991575403792 2210 84-85 0.12446610805703529 2210 86-87 0.20947641426880637 2210 88-89 0.23906170530518267 2210 90-91 0.1626359790453833 2210 92-93 0.24584477353508305 2210 94-95 0.17279819602598678 2210 96-97 0.10398482983723767 2210 98-99 0.09430902756281512 2210 100 -0.04097442433014109 2211 1 0.4433769794094431 2211 2 3.8535840205044494 2211 3 2.135813724591241 2211 4 0.8958077162268481 2211 5 0.5189698892083854 2211 6 0.26720389281971535 2211 7 0.2189800314585142 2211 8 0.3258591902103305 2211 9 0.27701605087171544 2211 10-11 0.2176104831849912 2211 12-13 0.15730801978774878 2211 14-15 0.22338305538023917 2211 16-17 0.17326177821047395 2211 18-19 0.2291741050589735 2211 20-21 0.34091187406844625 2211 22-23 0.12619752128324535 2211 24-25 0.1899617383463834 2211 26-27 0.1551255897794377 2211 28-29 0.11829979673746749 2211 30-31 0.0582775307627017 2211 32-33 0.234540213185241 2211 34-35 0.3108602715242341 2211 36-37 0.02457114944902372 2211 38-39 0.017224438374697115 2211 40-41 0.02653395295329375 2211 42-43 0.14435773922321005 2211 44-45 0.19758965236189852 2211 46-47 0.24741169027240062 2211 48-49 0.16211678560482312 2211 50-51 0.06313120792147942 2211 52-53 0.08734920414394765 2211 54-55 0.15257140277232395 2211 56-57 0.07962196376653452 2211 58-59 0.04833680627096726 2211 60-61 -0.0069596609353581584 2211 62-63 0.22768164487597886 2211 64-65 0.22657215863520008 2211 66-67 0.22622819313207287 2211 68-69 0.0900472004849533 2211 70-71 0.038232932922674934 2211 72-73 0.19794159674786016 2211 74-75 0.21882590806950475 2211 76-77 0.02617161052075545 2211 78-79 0.092063605670198 2211 80-81 0.19123402942611278 2211 82-83 -0.34474261218710467 2211 84-85 -0.03146611973338764 2211 86-87 0.07712157934673769 2211 88-89 0.1516915712883602 2211 90-91 0.15124691343064 2211 92-93 0.10842063999399443 2211 94-95 0.17318177953664815 2211 96-97 0.031264435900247634 2211 98-99 0.08796383581861278 2211 100 -0.016088878623243374 2212 1 0.4837942180640411 2212 2 4.077051946569014 2212 3 2.313771306768203 2212 4 1.1375318934773375 2212 5 0.5499935345434963 2212 6 0.3482370255081406 2212 7 0.34898728282527003 2212 8 0.410901731858921 2212 9 0.34534392489391763 2212 10-11 0.3415777870039065 2212 12-13 0.31091951667830386 2212 14-15 0.38375050547364253 2212 16-17 0.4984604113189306 2212 18-19 0.45651803809696645 2212 20-21 0.5281589336570391 2212 22-23 0.43547900348399793 2212 24-25 0.43118615923923187 2212 26-27 0.44236901923049743 2212 28-29 0.2726858714408067 2212 30-31 0.17657803399330163 2212 32-33 0.37618250076874915 2212 34-35 0.4926066705614289 2212 36-37 0.4005585245878791 2212 38-39 0.4944730524639098 2212 40-41 0.47598851922089835 2212 42-43 0.4463177017014033 2212 44-45 0.4189869524304086 2212 46-47 0.4604056673619965 2212 48-49 0.5668221119742327 2212 50-51 0.3364705424883212 2212 52-53 0.2974611192797951 2212 54-55 0.38766412049372434 2212 56-57 0.46225193382569785 2212 58-59 0.5651967907526867 2212 60-61 0.4316057176189574 2212 62-63 0.3593734582254484 2212 64-65 0.35722348102972035 2212 66-67 0.4644914907935487 2212 68-69 0.3809211015331968 2212 70-71 0.39012822104869116 2212 72-73 0.5200220976088517 2212 74-75 0.5971056148434783 2212 76-77 0.5669462952333006 2212 78-79 0.5516978125230025 2212 80-81 0.4427514491867086 2212 82-83 0.5404084104426872 2212 84-85 0.4686846534636402 2212 86-87 0.43052426538126554 2212 88-89 0.6782292129194047 2212 90-91 0.3916688066787657 2212 92-93 0.6401675289883997 2212 94-95 0.6638430836958449 2212 96-97 0.5049420413813479 2212 98-99 0.5713673146929921 2212 100 0.618291686288515 2213 1 -0.42471352589690525 2213 2 -25.951879804172826 2213 3 -14.81352287482729 2213 4 -6.172978423437758 2213 5 -2.073384923491105 2213 6 -1.216382273369021 2213 7 -0.992977183444502 2213 8 -0.9602300524431939 2213 9 -0.9883710250596849 2213 10-11 -0.9423268717067756 2213 12-13 -0.8945810333372606 2213 14-15 -1.1124926085753515 2213 16-17 -1.1618533485358142 2213 18-19 -1.2052127109998523 2213 20-21 -1.1213640873984403 2213 22-23 -1.2564484604351307 2213 24-25 -1.1140187376920068 2213 26-27 -1.2544314965076921 2213 28-29 -1.2435275761886757 2213 30-31 -1.273567931674826 2213 32-33 -1.2077538537539283 2213 34-35 -1.032071781879793 2213 36-37 -1.333315693235491 2213 38-39 -1.2844228432869897 2213 40-41 -1.3168910022727331 2213 42-43 -1.1411896306442486 2213 44-45 -1.0765664908677905 2213 46-47 -1.135308872410505 2213 48-49 -1.3656740551272364 2213 50-51 -1.12678203584408 2213 52-53 -1.2335089876971566 2213 54-55 -1.1855917493268464 2213 56-57 -1.1263527405177598 2213 58-59 -1.1689117394364956 2213 60-61 -1.1640557567669632 2213 62-63 -1.1680301528024017 2213 64-65 -1.104415622710242 2213 66-67 -1.1755577452800168 2213 68-69 -1.268893062048754 2213 70-71 -1.1303428274803267 2213 72-73 -1.1871824608512753 2213 74-75 -1.322100541315045 2213 76-77 -1.1050981420082806 2213 78-79 -1.1642252455348299 2213 80-81 -1.1149648675591521 2213 82-83 -1.236789930762015 2213 84-85 -1.1805872033802913 2213 86-87 -1.1897597276599967 2213 88-89 -1.094810487160764 2213 90-91 -0.998074407047099 2213 92-93 -0.9283816561710729 2213 94-95 -1.1700731920319036 2213 96-97 -1.3328150209762128 2213 98-99 -1.3282163012971893 2213 100 -1.330528899639404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 13.0 4 24.0 5 43.0 6 32.0 7 27.0 8 46.0 9 86.0 10 317.0 11 1509.0 12 4461.0 13 7936.0 14 10780.0 15 12464.0 16 13197.0 17 13930.0 18 14282.0 19 15151.0 20 17065.0 21 18722.0 22 20972.0 23 22807.0 24 23323.0 25 25018.0 26 27762.0 27 31676.0 28 37886.0 29 45363.0 30 55082.0 31 68504.0 32 88557.0 33 119377.0 34 165293.0 35 236838.0 36 364011.0 37 625275.0 38 647625.0 39 5024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.1533191200591 21.215568930404014 10.521701056599978 30.109410892936907 2 12.779009371062116 22.966094376980642 36.931139543467346 27.323756708489892 3 15.202858771353025 27.7778548121897 28.866934892380087 28.152351524077186 4 10.44090562584091 19.047292358567578 38.24697473339958 32.26482728219193 5 11.58302555377477 38.739471380612905 35.64286484441675 14.03463822119558 6 29.32912555146817 40.263894223256806 18.01265547349959 12.394324751775432 7 25.44594149165864 33.2667771717963 23.937878644634267 17.349402691910797 8 23.851606487863002 37.503832967315375 21.21076501647081 17.433795528350817 9 24.403405824214936 16.940635352309336 22.194913166084124 36.46104565739161 10-11 22.75960819780298 27.98927134392023 29.49639381724233 19.754726641034463 12-13 23.56057427180683 25.391590855703978 29.846707413519102 21.201127458970088 14-15 21.249182977159133 26.842519976790253 26.74339121837111 25.1649058276795 16-17 20.525507661067856 30.453130267081107 27.780348105753454 21.24101396609759 18-19 20.555543895924718 29.45550639246865 29.628168289231034 20.360781422375602 20-21 21.690566740149205 28.79976495320963 28.793870534475964 20.715797772165203 22-23 20.825965323362112 28.823107539185038 28.995560032629108 21.355367104823742 24-25 21.135392997210943 28.58349324852536 28.756869540590234 21.52424421367346 26-27 21.06674520202601 28.52731238643484 28.848296472441277 21.55764593909787 28-29 20.758008576009438 28.559885166584003 29.30135340947288 21.38075284793368 30-31 21.501816354232325 28.898920334079243 28.56077879312906 21.03848451855937 32-33 20.47550384691804 28.89728859283754 29.254293242272546 21.372914317971873 34-35 20.669050267261035 28.42326937168113 29.3706155907622 21.537064770295636 36-37 21.34418903116674 28.834372394857855 28.644671976283526 21.176766597691884 38-39 21.042386927686707 28.804784540233534 28.932962720493837 21.21986581158592 40-41 21.264613508918956 28.60878610510359 28.716802864487683 21.40979752148977 42-43 20.96023098209356 28.717176483296164 28.933667457456284 21.38892507715399 44-45 21.377661373873423 28.228453650684404 28.82547576568269 21.568409209759487 46-47 21.27139239379121 28.846141914876767 28.756563290245328 21.125902401086698 48-49 21.123096781352064 28.69191705451439 28.85110753542367 21.333878628709872 50-51 21.37407268716518 28.5673669096677 28.516160773398795 21.542399629768322 52-53 20.903593415630052 28.81782389047804 28.522816406248573 21.75576628764334 54-55 21.284152466963473 28.75032027347523 28.27372414361975 21.691803115941553 56-57 21.41306858886933 28.479433412005832 28.393741576342922 21.713756422781913 58-59 21.005513826718367 28.16390865846153 28.97316404825132 21.857413466568786 60-61 21.526976302777907 28.61638140796559 28.24510929691131 21.611532992345193 62-63 20.841516026643493 28.85890707075942 28.604603378574918 21.694973524022174 64-65 20.84411648024946 28.28612106345947 28.808055179541903 22.061707276749168 66-67 21.208099936787676 28.54315664539638 28.464232481207834 21.78451093660811 68-69 21.38853053803241 28.456174628078045 28.486870428137063 21.668424405752486 70-71 21.42766677409547 28.39038557208029 28.279373778879403 21.902573874944842 72-73 21.124758314924662 28.635641556967418 28.627137527133968 21.61246260097395 74-75 21.521929594847638 28.133519449185645 28.332342714737795 22.01220824122892 76-77 21.27123199880877 28.61032026326748 28.475814289421237 21.642633448502515 78-79 21.35750858535909 28.542970588986655 28.551711050205647 21.54780977544861 80-81 21.382921011993293 28.35113614861049 28.32841846806925 21.937524371326965 82-83 20.868542630680075 28.638150116046535 28.532066066241406 21.961241187031984 84-85 21.028293264331335 28.269105603383387 28.34898997722985 22.35361115505542 86-87 21.277018907350662 28.50936974159723 28.22454244255192 21.98906890850019 88-89 20.991682668552592 28.16864710531905 28.627100224719758 22.212570001408604 90-91 21.24085454970757 28.25067866731234 28.33645404129552 22.17201274168457 92-93 20.753424295120954 28.591511802280085 28.511814869571992 22.143249033026972 94-95 20.695113004395452 28.11676856307905 28.6704921989902 22.5176262335353 96-97 21.04242007590333 28.298593721235438 28.43488473374629 22.22410146911494 98-99 20.942646422660676 28.188951840481113 28.449163107508564 22.41923862934965 100 20.962682606171942 28.14002616239461 28.35723029299909 22.540060938434355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4.0 1 8.0 2 9.0 3 8.0 4 12.0 5 16.0 6 34.5 7 69.0 8 94.0 9 105.5 10 107.5 11 119.0 12 167.0 13 221.5 14 255.5 15 306.0 16 375.5 17 461.0 18 597.0 19 798.5 20 1111.0 21 1599.0 22 2359.5 23 3509.5 24 5305.0 25 8047.5 26 12254.5 27 18101.0 28 25785.5 29 35063.5 30 45625.0 31 56751.0 32 67753.0 33 78185.5 34 87529.5 35 96154.5 36 103602.5 37 109705.0 38 114084.0 39 116771.5 40 118600.0 41 118152.0 42 115027.5 43 110816.5 44 105936.5 45 100390.5 46 95332.0 47 91143.5 48 86736.5 49 84550.5 50 91951.5 51 87330.5 52 69618.5 53 60864.0 54 55668.0 55 51218.5 56 46748.5 57 42253.5 58 37977.5 59 33599.5 60 29081.0 61 24507.0 62 20219.5 63 16426.0 64 13003.5 65 10219.5 66 7897.0 67 5850.0 68 4410.0 69 3354.5 70 2410.0 71 1759.0 72 1327.5 73 984.5 74 706.5 75 495.5 76 310.5 77 201.0 78 143.0 79 81.0 80 43.5 81 23.0 82 14.0 83 10.0 84 7.0 85 3.5 86 2.0 87 1.5 88 1.5 89 1.0 90 1.0 91 1.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5763593066611008 2 4.498193380862755 3 0.0 4 0.005145087827743919 5 0.16358460093458146 6 0.05203471803094205 7 0.03419111556451101 8 0.03962812326900636 9 0.03842395377740672 10-11 0.03960987827670939 12-13 0.038077298923764394 14-15 0.04055861787615153 16-17 0.032092941450360124 18-19 0.02665593374586477 20-21 0.021821010787169248 22-23 0.021638560864199602 24-25 0.017661152543461397 26-27 0.011348385208711764 28-29 0.018573402158309608 30-31 0.023682000001459598 32-33 0.001368374422272319 34-35 0.0031381386750778516 36-37 0.0038132033900655287 38-39 0.005546477658277133 40-41 0.0059296224965133815 42-43 0.006531707242313203 44-45 0.007699386749318914 46-47 0.015581223421607473 48-49 0.02475845454698049 50-51 0.020872271187727106 52-53 0.02616331895384674 54-55 0.023061670263362814 56-57 0.015581223421607473 58-59 0.013373579353674796 60-61 0.01111120030885123 62-63 0.01631102311348604 64-65 0.017405722651303895 66-67 0.018463932204527824 68-69 0.025159844377513705 70-71 0.024776699539277455 72-73 0.021912235748654068 74-75 0.022167665640811567 76-77 0.016456983051861756 78-79 0.012698514638687118 80-81 0.011731530046948015 82-83 0.007225016949597845 84-85 0.009761070878875875 86-87 0.008064286595258199 88-89 0.0064222372885314166 90-91 0.006604687211501059 92-93 0.003521283513314101 94-95 0.0011494345147087478 96-97 6.750647149876773E-4 98-99 4.5612480742410633E-4 100 0.1080468443826223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2740478.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.85217977362063 #Duplication Level Percentage of deduplicated Percentage of total 1 90.56282402918472 77.75015849360337 2 7.355833623182695 12.630287012046479 3 1.264698305886347 3.2573131894904432 4 0.36223191193836873 1.243935968939006 5 0.15909978510258338 0.68295316762857 6 0.07468539840949275 0.3847142550429751 7 0.05143723753964863 0.3091199275018639 8 0.02969649198761987 0.20396068550136204 9 0.027317662682746328 0.21107527988708388 >10 0.10541381997239099 1.52430534908107 >50 0.004071746986369468 0.22733793717408352 >100 0.0016676445075529272 0.32264851682283524 >500 5.537689189767868E-4 0.29501806327599456 >1k 3.8337848215149335E-4 0.5068371559375525 >5k 8.51952182558874E-5 0.4503349980673224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7079 0.2583126009404199 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5235 0.19102506934921573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.6489984593928506E-5 0.0 10-11 0.0 0.0 0.0 3.6489984593928506E-5 0.0 12-13 0.0 0.0 0.0 3.6489984593928506E-5 0.0 14-15 0.0 0.0 0.0 9.122496148482126E-5 0.0 16-17 0.0 0.0 0.0 1.0946995378178552E-4 0.0 18-19 0.0 0.0 0.0 1.2771494607874976E-4 0.0 20-21 0.0 0.0 0.0 2.1893990756357103E-4 0.0 22-23 3.6489984593928506E-5 0.0 0.0 3.284098613453566E-4 0.0 24-25 3.6489984593928506E-5 0.0 0.0 3.8314483823624927E-4 0.0 26-27 3.6489984593928506E-5 0.0 0.0 4.7436979972107055E-4 0.0 28-29 3.6489984593928506E-5 0.0 0.0 7.480446841755344E-4 0.0 30-31 1.0946995378178552E-4 0.0 0.0 0.0024995639446841024 0.0 32-33 1.0946995378178552E-4 0.0 0.0 0.007772366718506772 0.0 34-35 1.2771494607874976E-4 0.0 0.0 0.015599468413904437 0.0 36-37 1.4595993837571402E-4 0.0 0.0 0.028316228044888522 0.0 38-39 1.4595993837571402E-4 0.0 0.0 0.05004601387057295 0.0 40-41 1.8244992296964253E-4 0.0 0.0 0.07885485670747949 0.0 42-43 1.8244992296964253E-4 0.0 0.0 0.10642304006819248 0.0 44-45 1.8244992296964253E-4 0.0 0.0 0.13955594607947958 0.0 46-47 1.8244992296964253E-4 0.0 0.0 0.17989562404806753 0.0 48-49 2.554298921574995E-4 0.0 0.0 0.22337344069173334 0.0 50-51 2.554298921574995E-4 0.0 0.0 0.27179565024787644 0.0 52-53 2.554298921574995E-4 0.0 0.0 0.3214037843033223 0.0 54-55 2.554298921574995E-4 0.0 0.0 0.3778902804547236 0.0 56-57 2.554298921574995E-4 0.0 0.0 0.4459441017224003 0.0 58-59 2.554298921574995E-4 0.0 0.0 0.524671243483801 0.0 60-61 2.554298921574995E-4 0.0 0.0 0.6067554638278432 0.0 62-63 2.554298921574995E-4 0.0 0.0 0.6907189183784727 0.0 64-65 2.554298921574995E-4 0.0 0.0 0.7854104283997172 0.0 66-67 2.554298921574995E-4 0.0 0.0 0.8930923729364002 0.0 68-69 2.554298921574995E-4 0.0 0.0 1.0168664006790058 0.0 70-71 2.554298921574995E-4 0.0 0.0 1.1643041834307737 0.0 72-73 2.554298921574995E-4 0.0 0.0 1.310665511637021 0.0 74-75 2.9191987675142804E-4 0.0 0.0 1.4635220571009875 0.0 76-77 2.9191987675142804E-4 0.0 0.0 1.6343681649697608 0.0 78-79 2.9191987675142804E-4 1.8244992296964253E-5 0.0 1.8302281572776722 0.0 80-81 2.9191987675142804E-4 3.6489984593928506E-5 0.0 2.048146345272613 0.0 82-83 2.9191987675142804E-4 3.6489984593928506E-5 0.0 2.283926380726282 0.0 84-85 2.9191987675142804E-4 3.6489984593928506E-5 0.0 2.5277889477675064 0.0 86-87 2.9191987675142804E-4 3.6489984593928506E-5 0.0 2.7839668846091814 0.0 88 2.9191987675142804E-4 3.6489984593928506E-5 0.0 3.000060573374426 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTAT 155 2.9442837E-5 22.254074 1 GTACCGT 200 1.41774035E-5 18.785906 6 GTATATA 2345 0.0 17.231148 1 TGTACCG 255 6.051163E-6 16.600954 5 ACCGTCC 235 5.6660305E-5 15.986546 8 GAGTACT 2880 0.0 15.898078 12-13 AGTACCG 245 7.978517E-5 15.358706 5 TATACCG 185 0.0033572337 15.254931 5 TAGCGTG 220 6.1454973E-4 14.966012 5 CTAGTAC 635 0.0 14.813959 3 CTAGCGT 195 0.0047642034 14.472629 4 GTCCTAG 1145 0.0 14.202071 1 TACCGAA 200 0.0056960373 14.088144 7 GTACATG 12620 0.0 13.939656 1 GTATCAA 10500 0.0 13.797527 1 TACACTG 2170 0.0 13.655625 5 GTACTAT 1085 0.0 13.624944 1 TACATGG 12745 0.0 13.562644 2 ACATGGG 12110 0.0 13.554895 3 CATGGGG 3620 0.0 13.383183 4 >>END_MODULE