FastQCFastQC Report
Wed 25 May 2016
SRR1295113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884020
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG73250.018.6013491
GTATATA20900.018.2896421
TACATGG78150.017.3517632
GTATCAA50550.017.0752371
ACATGGG79950.016.4760323
ACCGTGT5052.0008883E-1115.912188
GTACTAG6102.6011548E-1015.16076851
GTCTAGG7551.8189894E-1214.6989191
TATCAAC57100.013.9252662
GTGCTAC7553.092282E-1113.8823131
GTATAGA11200.013.7620091
GTATTCG3150.001198878913.7008441
TCAACGC56400.013.5587754
CATGGGG36950.013.4448074
ATCAACG56550.013.4234893
TAACGCA3805.2029347E-513.2201964
GTGTAGG9350.013.1879781
CAACGCA55050.013.12815
TATACTG13850.013.0451625
CCTATAC8153.6379788E-1213.0123313
Produced by FastQC (version 0.11.5)