##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295113_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2884020 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.50851173015444 27.0 22.0 33.0 6.0 33.0 2 21.686640175865634 27.0 6.0 33.0 2.0 33.0 3 23.924210303673345 27.0 15.0 33.0 6.0 33.0 4 24.222890617956878 33.0 6.0 37.0 2.0 37.0 5 25.818905555439976 33.0 15.0 37.0 2.0 37.0 6 26.628449525315357 33.0 15.0 37.0 6.0 37.0 7 26.19908946539899 33.0 15.0 37.0 6.0 37.0 8 27.058763462111912 33.0 15.0 37.0 6.0 37.0 9 28.15178812906984 33.0 22.0 37.0 15.0 37.0 10-11 27.73636659939945 33.0 18.5 37.0 6.0 37.0 12-13 26.56352989923787 33.0 15.0 37.0 4.0 37.0 14-15 28.12276163133404 33.0 15.0 37.0 6.0 40.0 16-17 28.522859064777638 33.0 22.0 37.0 6.0 40.0 18-19 28.66003287078453 33.0 18.5 37.0 6.0 40.0 20-21 28.177003626881923 33.0 15.0 37.0 6.0 40.0 22-23 28.88330698122759 33.0 22.0 37.0 6.0 40.0 24-25 29.008891755258283 33.0 22.0 37.0 6.0 40.0 26-27 27.686475648573868 33.0 15.0 37.0 4.0 40.0 28-29 28.367928620467264 33.0 18.5 37.0 6.0 40.0 30-31 29.05976120137863 33.0 22.0 37.0 6.0 40.0 32-33 27.912096136642603 33.0 18.5 37.0 6.0 40.0 34-35 27.426812227377063 33.0 15.0 37.0 6.0 40.0 36-37 27.653790542367943 33.0 15.0 37.0 6.0 40.0 38-39 27.78960305407036 33.0 18.5 37.0 6.0 40.0 40-41 26.91620983904411 33.0 15.0 37.0 6.0 40.0 42-43 27.448475565356688 33.0 15.0 37.0 6.0 40.0 44-45 27.242800674059126 33.0 15.0 37.0 6.0 40.0 46-47 27.021633518491548 33.0 15.0 37.0 6.0 40.0 48-49 26.823084271260257 33.0 15.0 37.0 6.0 40.0 50-51 26.73168597998627 33.0 15.0 37.0 6.0 40.0 52-53 26.96865971803247 33.0 18.5 37.0 6.0 40.0 54-55 26.930282556986427 33.0 15.0 37.0 6.0 40.0 56-57 26.8837726506751 33.0 15.0 37.0 6.0 40.0 58-59 26.743073210310605 33.0 15.0 37.0 6.0 40.0 60-61 26.743723344498306 33.0 18.5 37.0 6.0 40.0 62-63 26.690490010471493 27.0 18.5 37.0 6.0 38.5 64-65 26.23537960901797 27.0 15.0 37.0 6.0 37.0 66-67 26.44951439310407 27.0 18.5 37.0 6.0 37.0 68-69 26.067697866172914 27.0 18.5 37.0 6.0 37.0 70-71 26.18327057371308 27.0 18.5 37.0 6.0 37.0 72-73 26.21408936137752 27.0 18.5 33.0 6.0 37.0 74-75 26.32634430413104 27.0 22.0 33.0 6.0 37.0 76-77 26.100133147481642 27.0 22.0 33.0 6.0 37.0 78-79 25.730482971685355 27.0 18.5 33.0 6.0 37.0 80-81 25.522500364075142 27.0 22.0 33.0 6.0 37.0 82-83 25.621444199416093 27.0 22.0 33.0 6.0 37.0 84-85 25.572466730466502 27.0 22.0 33.0 6.0 37.0 86-87 25.36392206017989 27.0 18.5 33.0 6.0 37.0 88-89 25.317403312043606 27.0 22.0 33.0 6.0 37.0 90-91 25.433956942046173 27.0 22.0 33.0 6.0 37.0 92-93 25.375258146614797 27.0 22.0 33.0 6.0 37.0 94-95 25.244953571750543 27.0 22.0 33.0 6.0 37.0 96-97 25.315932275088244 27.0 22.0 33.0 6.0 37.0 98-99 25.37966987052794 27.0 22.0 33.0 6.0 37.0 100 25.067027621167675 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.332482627093718 1101 2 11.170326207545077 1101 3 8.288006529973078 1101 4 12.717796574778873 1101 5 10.621383152920046 1101 6 9.89366650313433 1101 7 9.476513559233656 1101 8 8.993397183654707 1101 9 7.372202722149041 1101 10-11 7.864008721372841 1101 12-13 9.116519223701857 1101 14-15 8.029792727627775 1101 16-17 8.170399915982507 1101 18-19 8.200315556207038 1101 20-21 7.163350594695142 1101 22-23 7.271919886134395 1101 24-25 7.340043861748406 1101 26-27 8.349394119093319 1101 28-29 8.111596988447861 1101 30-31 5.683252903990677 1101 32-33 6.96707171156487 1101 34-35 8.793921810780866 1101 36-37 8.281608661046722 1101 38-39 8.180619519675481 1101 40-41 8.980433225756393 1101 42-43 8.580269968310379 1101 44-45 8.3602365507024 1101 46-47 7.270127142829715 1101 48-49 5.3401380268574385 1101 50-51 6.85827409348278 1101 52-53 5.862600466257195 1101 54-55 4.0756154471794375 1101 56-57 6.196717349011319 1101 58-59 6.14694540294046 1101 60-61 6.654475009433746 1101 62-63 6.241974996618616 1101 64-65 6.621198406510082 1101 66-67 5.804661770166092 1101 68-69 6.59262972299889 1101 70-71 6.1109546264652295 1101 72-73 5.465122544494697 1101 74-75 4.976266722779798 1101 76-77 5.0487225565004366 1101 78-79 5.061838464714992 1101 80-81 4.9330651231904845 1101 82-83 4.426210454511946 1101 84-85 4.219695525726895 1101 86-87 3.9357968649293724 1101 88-89 4.0755209940904145 1101 90-91 3.8506648986821403 1101 92-93 3.784242932495438 1101 94-95 3.7923524265469553 1101 96-97 3.834019561972106 1101 98-99 3.539837416385545 1101 100 3.701051017108398 1102 1 6.628186994867445 1102 2 10.181908858666365 1102 3 7.860344245953009 1102 4 12.322245340977368 1102 5 10.216371480950883 1102 6 9.474503174780171 1102 7 6.727966695145817 1102 8 7.677296171006304 1102 9 6.444430306148419 1102 10-11 7.44741682320673 1102 12-13 9.048086141105706 1102 14-15 6.143687786601937 1102 16-17 7.132991214383999 1102 18-19 7.2570029115198835 1102 20-21 6.932524572508704 1102 22-23 6.460345398925067 1102 24-25 7.040860434812991 1102 26-27 8.362573784971076 1102 28-29 7.477923337190777 1102 30-31 4.264956486088913 1102 32-33 4.312134808096257 1102 34-35 5.025237768722981 1102 36-37 4.952784979720576 1102 38-39 4.539779105563067 1102 40-41 6.006540062264762 1102 42-43 6.714793858664997 1102 44-45 6.353671068045458 1102 46-47 5.504656327602483 1102 48-49 3.6430669515166514 1102 50-51 4.922570356189386 1102 52-53 5.559257889920577 1102 54-55 5.198686722980632 1102 56-57 6.240271819278515 1102 58-59 5.840141247105123 1102 60-61 5.807005882762162 1102 62-63 4.194604806860198 1102 64-65 5.896329716163223 1102 66-67 3.7885276370927095 1102 68-69 5.793993419618225 1102 70-71 5.133601298014078 1102 72-73 4.96830727846433 1102 74-75 4.104098125065043 1102 76-77 3.1174042948801954 1102 78-79 4.2314288828689435 1102 80-81 4.164609630251864 1102 82-83 3.766895754297259 1102 84-85 3.0651016480142133 1102 86-87 3.2400510107416913 1102 88-89 3.3276654355171758 1102 90-91 3.198407839727377 1102 92-93 3.116645397996848 1102 94-95 3.089354532997902 1102 96-97 3.2647487690541794 1102 98-99 3.0653800538302605 1102 100 3.2449358833172397 1103 1 6.531002350548587 1103 2 10.618595933906889 1103 3 7.688419025176625 1103 4 11.660775664985291 1103 5 9.960755889711969 1103 6 8.791480573839749 1103 7 7.796573485097433 1103 8 7.7145022473045195 1103 9 6.9531504498070404 1103 10-11 7.267382396954332 1103 12-13 8.591436666522291 1103 14-15 8.273862143283285 1103 16-17 7.705680334998441 1103 18-19 6.859247497940878 1103 20-21 8.01136548160894 1103 22-23 6.632161222067456 1103 24-25 5.047754038313059 1103 26-27 5.652941614563076 1103 28-29 6.743243483990447 1103 30-31 5.9472278026913585 1103 32-33 7.7876388224736175 1103 34-35 6.940030997699747 1103 36-37 4.023042626930835 1103 38-39 6.057087181618215 1103 40-41 6.5269303518608055 1103 42-43 7.249154364697443 1103 44-45 5.494273280079472 1103 46-47 6.024785671892108 1103 48-49 5.257853105445875 1103 50-51 6.218089600524014 1103 52-53 5.830981686120612 1103 54-55 4.702806019932879 1103 56-57 5.698116391800905 1103 58-59 5.198038711830993 1103 60-61 4.74875390092766 1103 62-63 2.470147871291431 1103 64-65 4.771456871986921 1103 66-67 3.838435168761823 1103 68-69 4.6595955905928435 1103 70-71 3.2836970716265164 1103 72-73 4.15474507183373 1103 74-75 3.0004663725390692 1103 76-77 3.3243237734997493 1103 78-79 2.8422965331340215 1103 80-81 3.8508563177849133 1103 82-83 3.177329245052327 1103 84-85 2.954476761440798 1103 86-87 2.5043745176372063 1103 88-89 2.645141321332634 1103 90-91 2.31165326540248 1103 92-93 1.9757908887921545 1103 94-95 2.654908454047245 1103 96-97 2.740452947997607 1103 98-99 2.5799836371455243 1103 100 2.6566897673310095 1104 1 6.113805258162305 1104 2 9.397046640448426 1104 3 6.998992487329204 1104 4 11.71629600428372 1104 5 9.001388699825558 1104 6 7.75309022655583 1104 7 3.9201220251981894 1104 8 6.379657044961679 1104 9 6.39991433302546 1104 10-11 7.041370566208357 1104 12-13 7.333099704304512 1104 14-15 6.952634367044251 1104 16-17 6.794813208366627 1104 18-19 6.566142909282867 1104 20-21 7.151581251646569 1104 22-23 6.121479689729075 1104 24-25 5.735925156950842 1104 26-27 7.930167642204232 1104 28-29 6.913892199041637 1104 30-31 6.17577161714938 1104 32-33 7.437767073271033 1104 34-35 7.83491307287521 1104 36-37 6.330120058713959 1104 38-39 7.0940927517777865 1104 40-41 6.816466941051274 1104 42-43 6.990433614768566 1104 44-45 6.303712678615671 1104 46-47 6.37531541132849 1104 48-49 5.1586320369132395 1104 50-51 5.371189690789681 1104 52-53 5.579813780971687 1104 54-55 4.4755655479720176 1104 56-57 5.6804194521450135 1104 58-59 3.5649190974548333 1104 60-61 3.8367591721008267 1104 62-63 3.6324727217755637 1104 64-65 2.779130774539105 1104 66-67 3.070787078228374 1104 68-69 3.881111023095656 1104 70-71 2.31948991457293 1104 72-73 2.2019690496893105 1104 74-75 2.930050229810803 1104 76-77 3.4941886495110275 1104 78-79 4.087679562696383 1104 80-81 2.9667697627079015 1104 82-83 2.78674338059637 1104 84-85 2.8994463636257173 1104 86-87 0.7368119664815858 1104 88-89 0.26303565158429265 1104 90-91 2.0344369881626996 1104 92-93 2.1353484899033646 1104 94-95 2.1770983216839284 1104 96-97 2.544131218936787 1104 98-99 2.284047922979127 1104 100 0.4970158217890095 1105 1 5.675041622420107 1105 2 -2.5056339388678417 1105 3 0.6029840450734909 1105 4 8.12746852857877 1105 5 8.155118048937585 1105 6 7.84976863238742 1105 7 4.943166193234397 1105 8 5.484452123989364 1105 9 4.668119675281975 1105 10-11 4.778312270780674 1105 12-13 4.803613668438651 1105 14-15 5.49634921849043 1105 16-17 5.6748194408870205 1105 18-19 5.880800039097174 1105 20-21 6.871072675910831 1105 22-23 2.055086413290258 1105 24-25 3.986962559582274 1105 26-27 6.607637443480826 1105 28-29 5.72305373214326 1105 30-31 5.135866345244999 1105 32-33 7.018854390441586 1105 34-35 6.010331295190532 1105 36-37 6.107487930964641 1105 38-39 3.9195340450348333 1105 40-41 5.0486530668446115 1105 42-43 5.279090841941734 1105 44-45 5.893184276370306 1105 46-47 5.012397703642758 1105 48-49 4.688118720111923 1105 50-51 5.737643596420465 1105 52-53 5.33250980564905 1105 54-55 5.161343231613216 1105 56-57 4.933151274327347 1105 58-59 1.7327314698493552 1105 60-61 4.011262169381254 1105 62-63 -0.7543975949333159 1105 64-65 -0.575325127983966 1105 66-67 0.40667359146335613 1105 68-69 2.417900902228265 1105 70-71 2.6055471294726686 1105 72-73 2.2141671045318674 1105 74-75 2.318286108051126 1105 76-77 1.49355536381276 1105 78-79 2.9749047990440722 1105 80-81 1.6290508832979818 1105 82-83 0.754011643861972 1105 84-85 1.112143809845957 1105 86-87 -0.5021753098034232 1105 88-89 1.3124420344412648 1105 90-91 1.2047656597503043 1105 92-93 1.4323527242368073 1105 94-95 0.8583128656470222 1105 96-97 1.8790802777278124 1105 98-99 1.6519790938928551 1105 100 1.8535538185425047 1106 1 5.723956221957515 1106 2 9.854581383276972 1106 3 6.351540106477685 1106 4 11.293549007250007 1106 5 9.356541544907532 1106 6 9.060653666870255 1106 7 6.068324366158212 1106 8 7.082509399188911 1106 9 6.668199663699962 1106 10-11 5.385634310417025 1106 12-13 7.494542786682793 1106 14-15 7.373889749507729 1106 16-17 7.333483401146204 1106 18-19 6.795594155072116 1106 20-21 7.8916762030328655 1106 22-23 5.857721373010246 1106 24-25 4.6047277997639995 1106 26-27 7.152688474037227 1106 28-29 5.6808065331630395 1106 30-31 4.926611045890333 1106 32-33 6.9128615965378195 1106 34-35 6.25440575832188 1106 36-37 5.477028336260176 1106 38-39 6.677434987686141 1106 40-41 6.085919876211644 1106 42-43 5.564834045275784 1106 44-45 4.1263904448100455 1106 46-47 5.549301856050818 1106 48-49 5.3075911930287205 1106 50-51 6.017429100608648 1106 52-53 5.187096202872613 1106 54-55 4.732391563671101 1106 56-57 4.426194426114449 1106 58-59 4.541961339820283 1106 60-61 4.54558245194297 1106 62-63 4.610473630903698 1106 64-65 4.629478129010884 1106 66-67 2.521586942821944 1106 68-69 2.7238044865773183 1106 70-71 2.4311708247177677 1106 72-73 2.5136759165849725 1106 74-75 2.432861325384664 1106 76-77 3.003624159501488 1106 78-79 3.6341425959570977 1106 80-81 3.1930225257475406 1106 82-83 1.6151366513023078 1106 84-85 2.1836333107690713 1106 86-87 2.87515257784651 1106 88-89 2.9502726581573064 1106 90-91 2.7709196764091537 1106 92-93 2.519442277728878 1106 94-95 1.1227671964716173 1106 96-97 1.1557604723233261 1106 98-99 1.6254611358382185 1106 100 2.4741324940462626 1107 1 5.69784320452905 1107 2 9.980827840790838 1107 3 5.564547940541331 1107 4 10.728500651348114 1107 5 8.856596824491017 1107 6 7.433188158481418 1107 7 3.1744224566938293 1107 8 5.816022356035958 1107 9 5.283847094681285 1107 10-11 4.122287689287347 1107 12-13 5.599518728502147 1107 14-15 5.518075102577164 1107 16-17 6.4147072249475485 1107 18-19 5.711478034986001 1107 20-21 5.0164185116396 1107 22-23 5.2310929153055135 1107 24-25 4.1971913541402905 1107 26-27 6.6654886423671975 1107 28-29 5.947936554397199 1107 30-31 4.536082391915613 1107 32-33 4.409136542136551 1107 34-35 6.190162872070964 1107 36-37 6.014520579935439 1107 38-39 5.931840407665973 1107 40-41 7.094347234022578 1107 42-43 5.578587291370344 1107 44-45 3.711191421062562 1107 46-47 3.0655666630136693 1107 48-49 3.4259807187549356 1107 50-51 4.195875505410804 1107 52-53 3.3809197985290957 1107 54-55 4.673936118925962 1107 56-57 4.811601791419491 1107 58-59 2.548252570347387 1107 60-61 2.305878667214216 1107 62-63 0.2273269156696962 1107 64-65 1.5880450016569228 1107 66-67 3.6020178552876736 1107 68-69 4.413397769628844 1107 70-71 3.682694718158359 1107 72-73 2.0670673068952397 1107 74-75 2.225417336046071 1107 76-77 2.5833891490900953 1107 78-79 3.531870345733111 1107 80-81 1.9148167602415676 1107 82-83 1.3166633745158833 1107 84-85 1.8360263597223572 1107 86-87 1.7629141354251097 1107 88-89 2.158497958678563 1107 90-91 1.0028643768958894 1107 92-93 1.8098033554852897 1107 94-95 1.8196098852388758 1107 96-97 1.8878135984687319 1107 98-99 1.3872938986816479 1107 100 -0.2502721453505927 1108 1 4.02356148544299 1108 2 -3.0115886756618284 1108 3 0.1703923458387706 1108 4 5.93214735832283 1108 5 6.203327198978801 1108 6 6.256464968766707 1108 7 4.031527378205432 1108 8 4.594488435961182 1108 9 4.623131963142246 1108 10-11 3.0624882267480373 1108 12-13 2.195646343156202 1108 14-15 3.5295543969987087 1108 16-17 4.328476155485255 1108 18-19 3.5552761231589685 1108 20-21 4.217153164022918 1108 22-23 3.7726998344261453 1108 24-25 2.5655225769438488 1108 26-27 4.240591529888732 1108 28-29 5.83254114010855 1108 30-31 4.28948284752218 1108 32-33 4.166051908755083 1108 34-35 2.6865138985250674 1108 36-37 0.8983800046152872 1108 38-39 2.0984221079626764 1108 40-41 5.094638217975898 1108 42-43 2.765014277578903 1108 44-45 3.3345228737428023 1108 46-47 4.244841110398504 1108 48-49 4.498030380359719 1108 50-51 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2212 84-85 -5.071327463806941 2212 86-87 -4.895725113948465 2212 88-89 -3.1418904077034533 2212 90-91 -3.950578281215236 2212 92-93 -5.129472539546626 2212 94-95 -3.38119644393392 2212 96-97 -5.124087271242882 2212 98-99 -3.568812705276507 2212 100 -3.5807441081344535 2213 1 -20.408962239826433 2213 2 -16.34583348827241 2213 3 -19.204547335679646 2213 4 -18.30976996514508 2213 5 -20.272786682314425 2213 6 -21.006523610934114 2213 7 -9.932496201393633 2213 8 -9.54794343618588 2213 9 -4.07003284828107 2213 10-11 -16.86040938893008 2213 12-13 -15.538823908617214 2213 14-15 -22.691452519520514 2213 16-17 -17.120872377868793 2213 18-19 -17.73806209312321 2213 20-21 -16.880361156735706 2213 22-23 -18.86702670691601 2213 24-25 -6.949881091542192 2213 26-27 -22.367177490539127 2213 28-29 -23.215874494441874 2213 30-31 -18.55820714648265 2213 32-33 -22.438821824556804 2213 34-35 -17.018249944773515 2213 36-37 -22.73383810701412 2213 38-39 -10.680869415509926 2213 40-41 -16.207328490752282 2213 42-43 -22.225576039294186 2213 44-45 -16.238579711522362 2213 46-47 -15.973384884941805 2213 48-49 -5.471995669922215 2213 50-51 -22.273379898912655 2213 52-53 -16.906364690684807 2213 54-55 -16.759486869990603 2213 56-57 -16.517906270683827 2213 58-59 -16.929653924667875 2213 60-61 -18.192163112152954 2213 62-63 -16.51511022866536 2213 64-65 -16.652487836551433 2213 66-67 -8.188535798227825 2213 68-69 -21.73119790691239 2213 70-71 -16.465558394630317 2213 72-73 -8.679761816765186 2213 74-75 -3.9149018634076462 2213 76-77 -2.5464042021945446 2213 78-79 -4.285886979084285 2213 80-81 -2.9329811754588206 2213 82-83 -2.9981520759950087 2213 84-85 -2.622997269437384 2213 86-87 -2.783264547748413 2213 88-89 -2.206192953361704 2213 90-91 -2.3559820613307743 2213 92-93 -3.3821288535858756 2213 94-95 -3.4611161163274033 2213 96-97 -2.9396605876897794 2213 98-99 -3.3279740702121714 2213 100 -2.832954348279813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 834.0 3 794.0 4 3702.0 5 12332.0 6 20650.0 7 25398.0 8 24345.0 9 25393.0 10 31326.0 11 39666.0 12 52671.0 13 68068.0 14 62432.0 15 69181.0 16 73986.0 17 74443.0 18 73044.0 19 71014.0 20 69507.0 21 68247.0 22 70250.0 23 75460.0 24 80574.0 25 86706.0 26 92469.0 27 98046.0 28 103781.0 29 107430.0 30 113435.0 31 122111.0 32 136642.0 33 159620.0 34 183698.0 35 201697.0 36 200002.0 37 147400.0 38 37617.0 39 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.97892369010246 21.55242141215028 14.14104845825555 24.32760643949171 2 14.696364403236473 22.218876223208216 40.11505938994764 22.969699983607676 3 18.111256854291966 27.2148846797865 29.778870974520505 24.89498749140103 4 12.051602622588298 18.29723269599403 39.0445149855736 30.606649695844073 5 12.963996313731982 37.84369162237376 35.49118095623567 13.70113110765859 6 29.60285063519923 38.36346674617543 19.38533370628683 12.64834891233851 7 25.538280890472915 31.77855071066559 24.1845855122283 18.49858288663319 8 24.175362740614474 36.29809927319593 22.020500183307316 17.506037802882272 9 24.343176286207004 17.510593045987626 22.653628884040664 35.492601783764705 10-11 22.96491482178869 27.738237161989016 29.37771810784412 19.919129908378178 12-13 23.716660491169424 25.44591514905265 29.34157656404568 21.49584779573225 14-15 21.227955999895272 26.998238350108277 26.81107707498794 24.96272857500851 16-17 20.907292722777004 30.58610007267634 27.512076601201663 20.994530603344987 18-19 20.768429908001742 29.615152157797166 29.009689111251014 20.60672882295008 20-21 22.21548191191973 28.89032595814681 28.069930322407888 20.82426180752557 22-23 21.25186692626618 28.943419706303285 28.715628103518426 21.089085263912107 24-25 21.476755884291578 28.874247517286335 28.189677680865167 21.459318917556928 26-27 21.443493148719117 28.89917613189543 28.282241316168427 21.375089403217025 28-29 21.21443136339701 28.73462837426156 28.797680565597545 21.253259696743886 30-31 21.78898084783343 29.103931899235906 28.149311048016074 20.95777620491459 32-33 20.89227277101491 29.078450905297288 28.75148626735286 21.277790056334943 34-35 21.003074522230573 28.787249843404684 28.421630983552742 21.788044650812004 36-37 21.59112669483884 29.221075958898123 28.022915764208946 21.16488158205409 38-39 21.35186654936193 28.965388048718143 28.26457750881028 21.418167893109647 40-41 21.5334149264099 28.89713358172888 27.81990532038844 21.749546171472787 42-43 21.279582631692215 29.001277190211116 28.47071078186671 21.24842939622996 44-45 21.66139626069361 28.477410289327644 28.407983921459156 21.453209528519587 46-47 21.555675134741865 28.886178508762043 28.437958098060673 21.120188258435416 48-49 21.421816786924868 28.713343000136277 28.474028338490005 21.39081187444885 50-51 21.755700865250187 28.675719970341902 28.197925934050378 21.37065323035753 52-53 21.2037058155064 28.758734265108956 28.436062880554385 21.60149703883026 54-55 21.665970312558315 28.644913110481813 28.20368858316061 21.485427993799263 56-57 21.739081384835387 28.63976735388517 28.1252769195861 21.49587434169334 58-59 21.455650925407745 28.344942011633023 28.418489458528228 21.780917604431004 60-61 21.875481942384553 28.711821154874485 27.996997003489444 21.41569989925152 62-63 21.400792660304102 28.82945662525536 28.373760865691604 21.395989848748933 64-65 21.472212291235167 28.276500952436635 28.416002823809844 21.835283932518355 66-67 21.5149652353171 28.59230462970158 28.16139362448025 21.731336510501066 68-69 21.966068994029836 28.421925126768688 28.13326967783427 21.47873620136721 70-71 21.981669643703878 28.532193380854636 27.844911575068753 21.64122540037273 72-73 21.599769535257003 28.725980130687024 28.332697748551634 21.34155258550434 74-75 21.835943156792567 28.2669375915022 28.036440694694413 21.860678557010818 76-77 21.882147683353733 28.692241464462025 27.97718415689824 21.448426695286006 78-79 21.82852033376076 28.405730011449815 28.190128517781538 21.575621137007893 80-81 21.996965708757237 28.321921559695777 27.975400757033192 21.705711974513793 82-83 21.461605875489628 28.56943917525917 28.431577510888456 21.537377438362746 84-85 21.672262747242982 28.16569071885991 28.05782326456513 22.10422326933198 86-87 21.866171362190006 28.521259573069678 28.046650802859386 21.565918261880928 88-89 21.6484984067458 28.097655181843038 28.373823893790494 21.880022517620663 90-91 21.90708275083646 28.168914741220245 28.070671326525844 21.853331181417452 92-93 21.569759943768922 28.4499848958929 28.305513850761688 21.67474130957649 94-95 21.545948612985473 27.980580298264485 28.25388545609573 22.21958563265431 96-97 21.71459873243579 28.198773693652 28.258221256441313 21.828406317470893 98-99 21.56876438040691 28.083702588629976 28.359747190443784 21.98778584051933 100 21.83174681090567 27.862258658199035 28.03562774767584 22.27036678321946 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 288.0 1 367.0 2 368.0 3 286.0 4 321.0 5 453.0 6 667.0 7 939.5 8 1354.5 9 1976.0 10 2732.0 11 3557.0 12 4472.0 13 5404.0 14 6378.5 15 7436.0 16 8271.0 17 9082.0 18 10122.5 19 11095.0 20 12009.0 21 13218.5 22 14852.0 23 16824.0 24 19334.5 25 22472.0 26 26853.5 27 32616.0 28 39568.0 29 47667.5 30 56295.0 31 65262.0 32 74067.0 33 82702.5 34 90569.5 35 97582.0 36 103787.0 37 108681.0 38 112302.0 39 114355.5 40 114304.0 41 113158.5 42 111298.5 43 107263.5 44 102416.5 45 97256.0 46 91688.0 47 86643.5 48 81727.5 49 77629.0 50 74614.0 51 69975.5 52 63665.5 53 58114.5 54 53555.0 55 49231.0 56 44718.5 57 40308.0 58 36145.0 59 31945.5 60 27734.5 61 23712.5 62 19986.0 63 16535.5 64 13448.0 65 10955.0 66 8878.0 67 6984.5 68 5424.5 69 4325.5 70 3408.5 71 2602.5 72 2020.5 73 1571.0 74 1209.0 75 882.5 76 633.5 77 454.5 78 325.5 79 249.5 80 174.5 81 125.0 82 87.0 83 52.5 84 41.5 85 32.5 86 20.0 87 10.5 88 7.5 89 8.0 90 5.5 91 4.0 92 3.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.575120838274353 2 24.69719350073855 3 5.946075269935715 4 23.232293812109486 5 12.44696638719565 6 9.756243021893052 7 7.927198840507348 8 8.25895798226087 9 5.198126226586501 10-11 6.637610002704558 12-13 9.883391932094785 14-15 7.2950256933030975 16-17 7.609742650883142 18-19 6.314831381197079 20-21 7.803656007933371 22-23 5.4882767803274595 24-25 5.047988571507825 26-27 9.391717117079631 28-29 6.100720522049084 30-31 3.9571327522000543 32-33 8.300618581008454 34-35 6.946935180754641 36-37 6.747699391821138 38-39 5.786384976525821 40-41 7.953585620071983 42-43 6.229013668421162 44-45 6.156528040720938 46-47 5.374633324318139 48-49 3.8233438048279833 50-51 6.423776534143314 52-53 4.222127447105082 54-55 4.77016456196559 56-57 4.721031754287418 58-59 3.376658275601417 60-61 3.7722345892192153 62-63 2.897934133605176 64-65 4.517773801846035 66-67 2.5029819488075673 68-69 4.893534025422848 70-71 2.377965478741479 72-73 1.4841956713198938 74-75 0.7533061490558318 76-77 1.0081067398977814 78-79 1.5035436647457368 80-81 2.0335677283791376 82-83 0.6528734197405012 84-85 1.0742470579260894 86-87 0.3967898974348305 88-89 0.5870971768573033 90-91 0.2068120193341239 92-93 0.25377771305330754 94-95 0.4906692741381821 96-97 0.4945874161760321 98-99 0.44819384054202116 100 0.2599843274318486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2884020.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.23385793865027 #Duplication Level Percentage of deduplicated Percentage of total 1 98.21106433822534 95.49440677866644 2 1.4605916827979133 2.840379283830929 3 0.1831885186178556 0.5343637918584108 4 0.059375305616116036 0.23093160125365483 5 0.03317095076437672 0.16126697571566842 6 0.016574939517017506 0.09669871886036405 7 0.009580404138083113 0.06520777584700616 8 0.00592508888339958 0.04608954006098806 9 0.0036579872430145747 0.03201121907358068 >10 0.01553612156718691 0.23651306320507937 >50 5.42659296531374E-4 0.04034622541747451 >100 7.205092809309382E-4 0.14854947228526288 >500 3.5747026114095995E-5 0.030691501716150853 >1k 3.574702611409514E-5 0.0425440522090235 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.934764668760966E-5 0.0 0.0 0.0 6 0.0 6.934764668760966E-5 0.0 0.0 0.0 7 0.0 6.934764668760966E-5 0.0 3.467382334380483E-5 0.0 8 0.0 6.934764668760966E-5 0.0 3.467382334380483E-5 0.0 9 0.0 6.934764668760966E-5 0.0 3.467382334380483E-5 0.0 10-11 0.0 8.668455835951207E-5 0.0 6.934764668760966E-5 0.0 12-13 1.7336911671902414E-5 1.2135838170331689E-4 0.0 1.0402147003141448E-4 0.0 14-15 3.467382334380483E-5 1.560322050471217E-4 0.0 1.560322050471217E-4 0.0 16-17 3.467382334380483E-5 1.7336911671902413E-4 0.0 1.7336911671902413E-4 0.0 18-19 3.467382334380483E-5 1.7336911671902413E-4 0.0 1.7336911671902413E-4 0.0 20-21 3.467382334380483E-5 1.7336911671902413E-4 0.0 2.0804294006282896E-4 0.0 22-23 3.467382334380483E-5 1.7336911671902413E-4 0.0 2.0804294006282896E-4 0.0 24-25 3.467382334380483E-5 1.9070602839092656E-4 0.0 2.427167634066338E-4 0.0 26-27 3.467382334380483E-5 2.0804294006282896E-4 0.0 2.9472749842234105E-4 0.0 28-29 3.467382334380483E-5 2.0804294006282896E-4 0.0 6.24128820188487E-4 0.0 30-31 3.467382334380483E-5 2.0804294006282896E-4 0.0 0.0014042898454240955 0.0 32-33 3.467382334380483E-5 2.0804294006282896E-4 0.0 0.004646292328069847 3.467382334380483E-5 34-35 3.467382334380483E-5 2.2537985173473138E-4 0.0 0.009812692006296767 3.467382334380483E-5 36-37 3.467382334380483E-5 2.9472749842234105E-4 0.0 0.017579628435309046 3.467382334380483E-5 38-39 3.467382334380483E-5 3.814120567818531E-4 0.0 0.029680792782296933 3.467382334380483E-5 40-41 6.934764668760966E-5 3.814120567818531E-4 0.0 0.04493727505357106 3.467382334380483E-5 42-43 6.934764668760966E-5 4.5075970346946276E-4 0.0 0.06047114791159562 3.467382334380483E-5 44-45 6.934764668760966E-5 4.5075970346946276E-4 0.0 0.07864023134374935 6.934764668760966E-5 46-47 6.934764668760966E-5 4.5075970346946276E-4 0.0 0.10131691181059771 6.934764668760966E-5 48-49 6.934764668760966E-5 4.854335268132676E-4 0.0 0.12557125123958918 8.668455835951207E-5 50-51 6.934764668760966E-5 4.854335268132676E-4 0.0 0.1523047690376627 1.0402147003141448E-4 52-53 6.934764668760966E-5 4.854335268132676E-4 0.0 0.18059860888620743 1.0402147003141448E-4 54-55 6.934764668760966E-5 5.547811735008773E-4 0.0 0.213902816207932 1.0402147003141448E-4 56-57 8.668455835951207E-5 5.547811735008773E-4 0.0 0.25452320025519937 1.0402147003141448E-4 58-59 1.0402147003141448E-4 5.547811735008773E-4 0.0 0.29866297737186287 1.0402147003141448E-4 60-61 1.386952933752193E-4 5.547811735008773E-4 0.0 0.3469116025547673 1.386952933752193E-4 62-63 1.386952933752193E-4 5.547811735008773E-4 0.0 0.39701527728656527 1.386952933752193E-4 64-65 1.386952933752193E-4 5.547811735008773E-4 0.0 0.4546778455073127 1.386952933752193E-4 66-67 1.386952933752193E-4 5.894549968446821E-4 0.0 0.5207661528006047 1.386952933752193E-4 68-69 1.386952933752193E-4 6.24128820188487E-4 0.0 0.5948814501979875 1.386952933752193E-4 70-71 1.386952933752193E-4 6.24128820188487E-4 0.0 0.6806991629739045 1.386952933752193E-4 72-73 1.386952933752193E-4 6.588026435322917E-4 0.0 0.7717179492513921 1.386952933752193E-4 74-75 1.7336911671902413E-4 6.761395552041941E-4 0.0 0.8678337875604192 1.386952933752193E-4 76-77 1.7336911671902413E-4 6.934764668760965E-4 0.0 0.978894043730626 1.386952933752193E-4 78-79 1.7336911671902413E-4 6.934764668760965E-4 0.0 1.1104291925853498 1.386952933752193E-4 80-81 1.7336911671902413E-4 7.10813378547999E-4 0.0 1.2504247543359615 1.386952933752193E-4 82-83 1.7336911671902413E-4 7.281502902199014E-4 0.0 1.40669967614649 1.386952933752193E-4 84-85 1.7336911671902413E-4 7.281502902199014E-4 0.0 1.5676382271967602 1.386952933752193E-4 86-87 1.7336911671902413E-4 7.281502902199014E-4 0.0 1.748670258874765 1.386952933752193E-4 88 1.7336911671902413E-4 7.281502902199014E-4 0.0 1.900576278943974 1.386952933752193E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7325 0.0 18.601349 1 GTATATA 2090 0.0 18.289642 1 TACATGG 7815 0.0 17.351763 2 GTATCAA 5055 0.0 17.075237 1 ACATGGG 7995 0.0 16.476032 3 ACCGTGT 505 2.0008883E-11 15.91218 8 GTACTAG 610 2.6011548E-10 15.1607685 1 GTCTAGG 755 1.8189894E-12 14.698919 1 TATCAAC 5710 0.0 13.925266 2 GTGCTAC 755 3.092282E-11 13.882313 1 GTATAGA 1120 0.0 13.762009 1 GTATTCG 315 0.0011988789 13.700844 1 TCAACGC 5640 0.0 13.558775 4 CATGGGG 3695 0.0 13.444807 4 ATCAACG 5655 0.0 13.423489 3 TAACGCA 380 5.2029347E-5 13.220196 4 GTGTAGG 935 0.0 13.187978 1 CAACGCA 5505 0.0 13.1281 5 TATACTG 1385 0.0 13.045162 5 CCTATAC 815 3.6379788E-12 13.012331 3 >>END_MODULE