FastQCFastQC Report
Wed 25 May 2016
SRR1295113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884020
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35910.12451369962760313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72050.023.4523721
TCAACGC80050.020.7928664
ATCAACG80900.020.6325193
CAACGCA80400.020.5269075
AACGCAG81800.020.3219136
TATCAAC83200.019.9327322
TACACCG2251.8402188E-618.8075585
TAGGGCG1300.00802317118.084195
ACGCAGA92450.018.0297977
CGCAGAG92900.017.841388
GAGTACT34650.016.46341112-13
GTACATG96000.016.2594451
GGTATCA53600.015.9936561
ACATGGG93150.015.6477273
TACATGG97600.015.6223442
GCAGAGT105750.015.450889
GTACTAT11400.015.2135171
GTATTAG14250.014.2572381
CATGGGG40850.014.1574644
TATATCG2000.00564937414.1056695
Produced by FastQC (version 0.11.5)