##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295113_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2884020 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.309640016366046 33.0 33.0 33.0 27.0 33.0 2 30.05979604857109 33.0 33.0 33.0 27.0 33.0 3 30.858959022475574 33.0 33.0 33.0 27.0 33.0 4 35.0808038779204 37.0 37.0 37.0 33.0 37.0 5 35.408567901748256 37.0 37.0 37.0 33.0 37.0 6 35.17548075256066 37.0 37.0 37.0 33.0 37.0 7 35.05591882164479 37.0 37.0 37.0 33.0 37.0 8 35.05097121379186 37.0 37.0 37.0 33.0 37.0 9 35.00371530017129 37.0 37.0 37.0 33.0 37.0 10-11 34.98972874667999 37.0 37.0 37.0 33.0 37.0 12-13 34.90929379823996 37.0 37.0 37.0 33.0 37.0 14-15 36.35163053654274 37.0 37.0 40.0 33.0 40.0 16-17 36.34530551105748 37.0 37.0 40.0 33.0 40.0 18-19 36.36247234762588 38.5 37.0 40.0 30.0 40.0 20-21 36.35927854175769 40.0 37.0 40.0 27.0 40.0 22-23 36.31895409879266 38.5 37.0 40.0 27.0 40.0 24-25 36.29716714863281 37.0 37.0 40.0 27.0 40.0 26-27 36.2788657845646 37.0 37.0 40.0 27.0 40.0 28-29 36.2173814675349 37.0 37.0 40.0 27.0 40.0 30-31 36.12656881713719 37.0 37.0 40.0 27.0 40.0 32-33 36.09226895097815 37.0 37.0 40.0 27.0 40.0 34-35 35.997246378319154 37.0 37.0 40.0 27.0 40.0 36-37 35.99381366980812 37.0 37.0 40.0 27.0 40.0 38-39 35.925721735632905 37.0 37.0 40.0 27.0 40.0 40-41 35.86471297009036 37.0 37.0 40.0 27.0 40.0 42-43 35.77773645813829 37.0 37.0 40.0 27.0 40.0 44-45 35.6808453131393 37.0 37.0 40.0 27.0 40.0 46-47 35.58490007004112 37.0 37.0 40.0 27.0 40.0 48-49 35.483626673878824 37.0 37.0 40.0 27.0 40.0 50-51 35.38573397549254 37.0 37.0 40.0 27.0 40.0 52-53 35.28547374844835 37.0 33.0 40.0 27.0 40.0 54-55 35.17161982233133 37.0 33.0 40.0 27.0 40.0 56-57 35.0516633033058 37.0 33.0 40.0 27.0 40.0 58-59 34.93153740265324 37.0 33.0 40.0 27.0 40.0 60-61 34.77823558782533 37.0 33.0 40.0 27.0 40.0 62-63 34.599842060734666 37.0 33.0 40.0 27.0 40.0 64-65 34.42954764530066 37.0 33.0 40.0 27.0 40.0 66-67 34.218517035249405 37.0 33.0 40.0 27.0 40.0 68-69 34.00523696090873 37.0 33.0 38.5 24.5 40.0 70-71 33.779024764044635 37.0 33.0 37.0 22.0 40.0 72-73 33.56381717186427 37.0 33.0 37.0 22.0 40.0 74-75 33.33053151503804 37.0 33.0 37.0 22.0 40.0 76-77 33.09309418797373 37.0 33.0 37.0 22.0 38.5 78-79 32.85624267515482 37.0 33.0 37.0 22.0 37.0 80-81 32.634814772435696 37.0 33.0 37.0 22.0 37.0 82-83 32.41583276121525 37.0 33.0 37.0 22.0 37.0 84-85 32.240327736978244 33.0 33.0 37.0 22.0 37.0 86-87 32.258380489733085 33.0 33.0 37.0 22.0 37.0 88-89 32.266582756014174 33.0 33.0 37.0 22.0 37.0 90-91 32.26391685910639 33.0 33.0 37.0 22.0 37.0 92-93 32.23350358180595 33.0 33.0 37.0 22.0 37.0 94-95 32.25083910652492 33.0 33.0 37.0 22.0 37.0 96-97 32.20851571764412 33.0 33.0 37.0 22.0 37.0 98-99 32.13227196760078 33.0 33.0 37.0 22.0 37.0 100 31.603118216933307 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6343255789210538 1101 2 4.123152677548557 1101 3 2.3887413920192984 1101 4 1.2081014279070246 1101 5 0.5393203469549803 1101 6 0.6503539535386551 1101 7 0.6382412176085381 1101 8 0.5990518624415699 1101 9 0.642262913547242 1101 10-11 0.6782782484764098 1101 12-13 0.7301519805267063 1101 14-15 1.008626648791875 1101 16-17 0.9895011711381159 1101 18-19 1.027636274399562 1101 20-21 0.9970179344462409 1101 22-23 0.9498484116659753 1101 24-25 0.9424272508866167 1101 26-27 1.002623512880504 1101 28-29 0.9362823880909517 1101 30-31 0.8169487769417714 1101 32-33 1.0352082091771493 1101 34-35 1.159516795787745 1101 36-37 1.0496858215972793 1101 38-39 1.048926547944312 1101 40-41 1.0727275740723883 1101 42-43 1.1539587368565023 1101 44-45 1.099857968906349 1101 46-47 1.0698340537339845 1101 48-49 1.0998409343171645 1101 50-51 1.0722534533016557 1101 52-53 1.0625793848642644 1101 54-55 1.003543697803103 1101 56-57 0.9824425307697666 1101 58-59 1.0647717315032565 1101 60-61 1.0451590767767698 1101 62-63 1.025205175656076 1101 64-65 1.0326945016760476 1101 66-67 0.9988145304349487 1101 68-69 0.9906162068986646 1101 70-71 0.9632926340977903 1101 72-73 0.9507480503869203 1101 74-75 0.9673993182384919 1101 76-77 0.928113339118795 1101 78-79 0.9284569222733268 1101 80-81 0.8481819194202203 1101 82-83 0.9006727673810104 1101 84-85 0.8850678817389124 1101 86-87 0.9211519905992844 1101 88-89 0.8923368415428854 1101 90-91 0.9136837153817865 1101 92-93 1.0002258554388632 1101 94-95 0.9086507501153065 1101 96-97 0.8102572630602545 1101 98-99 0.8729848006078846 1101 100 0.9857038899911466 1102 1 0.5526153282808757 1102 2 4.028834032790275 1102 3 2.294400720864907 1102 4 1.1283404122840466 1102 5 0.4658439269192911 1102 6 0.505392784660458 1102 7 0.5049829356836 1102 8 0.5434115692520507 1102 9 0.5043661555094232 1102 10-11 0.6701023129405925 1102 12-13 0.7012177832736555 1102 14-15 0.8986580475205699 1102 16-17 0.8990879680717185 1102 18-19 0.854898910680312 1102 20-21 0.8682593017155824 1102 22-23 0.9194992710591592 1102 24-25 0.953234128295378 1102 26-27 0.8644943662598763 1102 28-29 0.9015816197132622 1102 30-31 0.8548148798943629 1102 32-33 1.0002459531245407 1102 34-35 1.0449720591668594 1102 36-37 0.8798530120475974 1102 38-39 0.9134257161952632 1102 40-41 0.9466887971072282 1102 42-43 0.9593008653904249 1102 44-45 0.9700171126494013 1102 46-47 0.9980962034132475 1102 48-49 0.9427087670491119 1102 50-51 0.8293731322093407 1102 52-53 0.875653788817857 1102 54-55 0.8248791117215148 1102 56-57 0.8075270797985112 1102 58-59 0.9062659456337769 1102 60-61 0.88061300644749 1102 62-63 0.9503202787315246 1102 64-65 0.8462034722794982 1102 66-67 0.928589832903846 1102 68-69 0.8315724919792373 1102 70-71 0.8233726808503334 1102 72-73 0.9058229926737198 1102 74-75 0.8149226015537252 1102 76-77 0.7363852814508647 1102 78-79 0.6749843295765459 1102 80-81 0.7576395891325873 1102 82-83 0.7404704438185092 1102 84-85 0.7230027110889026 1102 86-87 0.8578972328977272 1102 88-89 0.7715143799536861 1102 90-91 0.8026018717603094 1102 92-93 0.8652536571964546 1102 94-95 0.8138280242093501 1102 96-97 0.8474446903125248 1102 98-99 0.8935848360117866 1102 100 0.7654110318783189 1103 1 0.39104864946062534 1103 2 3.933654498518816 1103 3 2.22807519107549 1103 4 0.9356289994958473 1103 5 0.3524035332582045 1103 6 0.4237949294391825 1103 7 0.398015889751278 1103 8 0.4236268826830738 1103 9 0.47580967769135185 1103 10-11 0.45649663210597424 1103 12-13 0.5425028849531586 1103 14-15 0.7810148523562148 1103 16-17 0.7250532822212818 1103 18-19 0.7374967855074459 1103 20-21 0.7493463574939554 1103 22-23 0.644657154709968 1103 24-25 0.6587027730806696 1103 26-27 0.6720414480452774 1103 28-29 0.6683314034328802 1103 30-31 0.605537717768037 1103 32-33 0.70999477071652 1103 34-35 0.8832229996323733 1103 36-37 0.7361478899697929 1103 38-39 0.7004404685290808 1103 40-41 0.7085335224732319 1103 42-43 0.7767837697967579 1103 44-45 0.7703660841730624 1103 46-47 0.8020526279978029 1103 48-49 0.6948141261833527 1103 50-51 0.6886859657143063 1103 52-53 0.7358618711756293 1103 54-55 0.7425713651680397 1103 56-57 0.704680575431432 1103 58-59 0.7223639550330887 1103 60-61 0.6935283735842077 1103 62-63 0.8002650127815869 1103 64-65 0.7579620781240592 1103 66-67 0.671215218554245 1103 68-69 0.7246227423183882 1103 70-71 0.7242425909220387 1103 72-73 0.7001496026332532 1103 74-75 0.7378119098301497 1103 76-77 0.7098821328109182 1103 78-79 0.698810495273392 1103 80-81 0.6998668217989561 1103 82-83 0.713150200323966 1103 84-85 0.7104187497520726 1103 86-87 0.6876047904137366 1103 88-89 0.6304402157998581 1103 90-91 0.6629259212557699 1103 92-93 0.6918260602100474 1103 94-95 0.7076605459010281 1103 96-97 0.6850448649792007 1103 98-99 0.7258724506290939 1103 100 0.7811207425491666 1104 1 0.43899086803821064 1104 2 3.557227680072195 1104 3 2.1472073820450674 1104 4 1.0056383316529178 1104 5 0.4149885757281311 1104 6 0.3375862312265383 1104 7 0.3498131943156153 1104 8 0.33117308475929974 1104 9 0.39848503244475353 1104 10-11 0.4716185205398844 1104 12-13 0.5153144798766789 1104 14-15 0.5930448406026443 1104 16-17 0.6079748391415478 1104 18-19 0.6311976248059494 1104 20-21 0.6094910156400957 1104 22-23 0.6562505373339746 1104 24-25 0.6304008281410418 1104 26-27 0.6495279266470249 1104 28-29 0.6229079220060996 1104 30-31 0.5593507527209596 1104 32-33 0.6885087836489419 1104 34-35 0.8065454158828445 1104 36-37 0.7117008497345694 1104 38-39 0.6006359686087777 1104 40-41 0.5731882527321943 1104 42-43 0.6326886866794439 1104 44-45 0.7155313418225617 1104 46-47 0.6875365463746448 1104 48-49 0.5478489936445712 1104 50-51 0.5814911794986557 1104 52-53 0.5518471168630796 1104 54-55 0.6413004333676326 1104 56-57 0.6566409952761987 1104 58-59 0.6257697844782442 1104 60-61 0.6036659293463345 1104 62-63 0.6352181558228267 1104 64-65 0.5890293430743156 1104 66-67 0.5661713993699351 1104 68-69 0.6052998293542515 1104 70-71 0.5628479002421898 1104 72-73 0.5400393363411169 1104 74-75 0.5862875129144882 1104 76-77 0.5512799415474063 1104 78-79 0.5092325866529137 1104 80-81 0.5665185844656335 1104 82-83 0.5750431196808918 1104 84-85 0.6956195439242094 1104 86-87 0.6164187544387616 1104 88-89 0.5558124953180936 1104 90-91 0.5295569400406279 1104 92-93 0.5957148906137917 1104 94-95 0.5622121769955655 1104 96-97 0.5541781630660658 1104 98-99 0.5728167874910817 1104 100 0.5952225939729878 1105 1 0.532258891531729 1105 2 3.7483074855927008 1105 3 2.1775768459576845 1105 4 1.0277683621733686 1105 5 0.3941613413488554 1105 6 0.3532456005534712 1105 7 0.3300580725536051 1105 8 0.2388344499427859 1105 9 0.33446974606859925 1105 10-11 0.31540253302460286 1105 12-13 0.3908714126687727 1105 14-15 0.4974830191530941 1105 16-17 0.5017959179035643 1105 18-19 0.5106180211238183 1105 20-21 0.4393446718147018 1105 22-23 0.5078332790266913 1105 24-25 0.5038591467691376 1105 26-27 0.48798757489673505 1105 28-29 0.4689986920499223 1105 30-31 0.4490320863094297 1105 32-33 0.5611345028033412 1105 34-35 0.644564230481798 1105 36-37 0.5395805939397107 1105 38-39 0.5553016716989632 1105 40-41 0.5304138852475688 1105 42-43 0.5483036489749011 1105 44-45 0.5511596210487966 1105 46-47 0.4848481343312514 1105 48-49 0.4590651481717245 1105 50-51 0.40169565381132344 1105 52-53 0.4667395909976051 1105 54-55 0.401615552395306 1105 56-57 0.45041070170558584 1105 58-59 0.42474303694503135 1105 60-61 0.4166942014778101 1105 62-63 0.4598026523053491 1105 64-65 0.369169275715457 1105 66-67 0.3301830865756088 1105 68-69 0.3737946200081126 1105 70-71 0.4681621272452361 1105 72-73 0.5276290599067011 1105 74-75 0.516318175775524 1105 76-77 0.29419074089727815 1105 78-79 0.41053187425019644 1105 80-81 0.42305162264973006 1105 82-83 0.47555481038216385 1105 84-85 0.41821750544726655 1105 86-87 0.4921251304857961 1105 88-89 0.4616028097172915 1105 90-91 0.4412982886316925 1105 92-93 0.395532938304612 1105 94-95 0.40314963402884985 1105 96-97 0.3906019495953821 1105 98-99 0.48599146041323493 1105 100 0.3573713867879036 1106 1 0.3910397119165694 1106 2 3.712128685620293 1106 3 2.102652709130293 1106 4 0.9483808716223976 1106 5 0.36087378244694435 1106 6 0.16206673356026613 1106 7 0.13670213395410258 1106 8 0.1161097994536604 1106 9 0.15421049217898997 1106 10-11 0.14574643083787464 1106 12-13 0.1752058825823184 1106 14-15 0.23165984640311876 1106 16-17 0.21765769565758575 1106 18-19 0.22201399286086598 1106 20-21 0.2081901998766611 1106 22-23 0.16467112809781526 1106 24-25 0.17917383250401286 1106 26-27 0.13815259241535216 1106 28-29 0.1314258184925805 1106 30-31 0.08784126496369993 1106 32-33 0.16923267403956999 1106 34-35 0.30305993260009956 1106 36-37 0.14115258333614378 1106 38-39 0.11998824112744444 1106 40-41 0.22104142276136685 1106 42-43 0.14994869360381102 1106 44-45 0.10600611287490125 1106 46-47 0.07852126103203005 1106 48-49 -0.0056779857791084964 1106 50-51 0.13174945687157447 1106 52-53 0.06136117747581693 1106 54-55 0.014748050860127648 1106 56-57 0.17655200547006444 1106 58-59 0.18295358484695612 1106 60-61 0.12732964128237256 1106 62-63 0.1291005270682959 1106 64-65 0.10649285760040783 1106 66-67 -0.007399442084512486 1106 68-69 -0.009856499734723911 1106 70-71 0.05824034590342109 1106 72-73 0.017717300869719566 1106 74-75 -0.0653716587724773 1106 76-77 -0.030143510235745907 1106 78-79 0.02741733170870475 1106 80-81 0.031364992260570546 1106 82-83 0.0817382540481546 1106 84-85 0.07416843318195276 1106 86-87 0.08909309952824884 1106 88-89 0.07755931019241302 1106 90-91 0.08242777336915452 1106 92-93 0.11293108428863974 1106 94-95 0.07371072235203968 1106 96-97 0.07173915019796695 1106 98-99 0.037823056521382625 1106 100 0.024037796669922784 1107 1 0.37317422437734393 1107 2 3.5123762236858482 1107 3 2.0693089893164007 1107 4 0.9227751948039398 1107 5 0.2860234889362516 1107 6 0.17229886054157362 1107 7 0.03303974337465121 1107 8 0.13754110757658822 1107 9 0.12638410149887136 1107 10-11 0.07919008060370203 1107 12-13 0.06338118746640475 1107 14-15 0.007958332136013269 1107 16-17 -0.047849901101173486 1107 18-19 0.004768436402926568 1107 20-21 0.0869420029786383 1107 22-23 0.07393593263332576 1107 24-25 0.0645722038578711 1107 26-27 0.03445224582884521 1107 28-29 -0.004752822840778492 1107 30-31 -0.11132886418253918 1107 32-33 -0.031168808787029434 1107 34-35 0.22456504477984396 1107 36-37 0.004600463069643013 1107 38-39 -0.08900059985757736 1107 40-41 -0.047628787062144795 1107 42-43 0.10011202196874791 1107 44-45 0.17064362150954793 1107 46-47 -0.004349158488516025 1107 48-49 -0.0973736687975233 1107 50-51 -0.04084958933523808 1107 52-53 0.0036077208410318917 1107 54-55 -0.0866330316666577 1107 56-57 -0.06018024933010224 1107 58-59 -0.04774402254484755 1107 60-61 -0.05185536620982134 1107 62-63 0.053626188765676375 1107 64-65 -0.052073337944193554 1107 66-67 0.02909233950095569 1107 68-69 -0.07208990222183331 1107 70-71 -0.03686037804576614 1107 72-73 0.039322560184871236 1107 74-75 0.055892609703100504 1107 76-77 -0.05783634585097275 1107 78-79 -0.05565343318468052 1107 80-81 0.02194068562202034 1107 82-83 0.16417212199779385 1107 84-85 0.13385621532086134 1107 86-87 0.07859927922866916 1107 88-89 0.010907705838889115 1107 90-91 0.06593448938502178 1107 92-93 0.09866437812012663 1107 94-95 -0.03555575577382086 1107 96-97 -0.04143789534784048 1107 98-99 0.09803335656291878 1107 100 0.05706759692627372 1108 1 0.47194325729709874 1108 2 3.5217218375422235 1108 3 2.0795812444375343 1108 4 0.859582031297748 1108 5 0.3244048325828217 1108 6 0.12886584486733454 1108 7 0.05811569604607314 1108 8 0.03128319469971785 1108 9 4.766649174641202E-4 1108 10-11 0.02340431639639462 1108 12-13 0.003504674385261808 1108 14-15 -0.05467233537048344 1108 16-17 -0.04425426924744613 1108 18-19 0.038799895123069916 1108 20-21 0.038167026748659794 1108 22-23 -0.06885326092194077 1108 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0.18857079089545437 2210 62-63 0.2516551074533524 2210 64-65 -0.17944050910593745 2210 66-67 0.017242147784692463 2210 68-69 0.11386765670967947 2210 70-71 0.172240456200619 2210 72-73 0.15050853564885358 2210 74-75 -0.38801685925283635 2210 76-77 0.019604124102286846 2210 78-79 0.24407868626776263 2210 80-81 0.1300456532895069 2210 82-83 0.15139032519228124 2210 84-85 0.1997561864625368 2210 86-87 0.24118690121104436 2210 88-89 0.3977128382124704 2210 90-91 0.2807608867163509 2210 92-93 0.35662914044247884 2210 94-95 0.2571379502286817 2210 96-97 0.19635359154773013 2210 98-99 0.24178213788360736 2210 100 0.10177694339540366 2211 1 0.4910165488102507 2211 2 3.9365075899373316 2211 3 2.124978152697679 2211 4 0.9880115061699968 2211 5 0.5005215089929891 2211 6 0.36105029685675305 2211 7 0.26280021744047133 2211 8 0.23341749112977084 2211 9 0.21387916076298552 2211 10-11 0.2008522677483029 2211 12-13 0.1973597493220609 2211 14-15 0.23925478358709285 2211 16-17 0.19782257925300684 2211 18-19 0.3070036853489313 2211 20-21 0.34153546898931353 2211 22-23 0.12588636684131416 2211 24-25 0.2112174589034268 2211 26-27 0.2555989014656177 2211 28-29 0.20510103495877985 2211 30-31 0.11409249613417671 2211 32-33 0.23360133723275567 2211 34-35 0.33749036298206647 2211 36-37 0.2712338109122854 2211 38-39 0.13286140606589214 2211 40-41 0.11303723472911287 2211 42-43 0.272556734575069 2211 44-45 0.18447391538298774 2211 46-47 0.28830105106460024 2211 48-49 0.2181743179600133 2211 50-51 0.04332531895363445 2211 52-53 0.1356817658459306 2211 54-55 0.16017519998187169 2211 56-57 0.1369895653219757 2211 58-59 0.18601423957792917 2211 60-61 0.22619460684713033 2211 62-63 0.28109409075559455 2211 64-65 0.28463933710617084 2211 66-67 0.10854386896917134 2211 68-69 0.09310763657956755 2211 70-71 0.09328452588090386 2211 72-73 0.17440389193654937 2211 74-75 0.10229950881839045 2211 76-77 0.06152632452643303 2211 78-79 0.09361882202024674 2211 80-81 0.05140131381291013 2211 82-83 -0.23444706149955152 2211 84-85 -0.018263316234993 2211 86-87 0.1789974101415126 2211 88-89 0.29308755733548963 2211 90-91 0.3428244400400402 2211 92-93 0.2789393125634163 2211 94-95 0.3620350013364302 2211 96-97 0.20524264814605075 2211 98-99 0.2250699343697029 2211 100 0.09336206031233374 2212 1 0.592568251360813 2212 2 4.151310414725671 2212 3 2.382870870986885 2212 4 1.2903968004898303 2212 5 0.6942804257386896 2212 6 0.6706877012348684 2212 7 0.5325277255030372 2212 8 0.5046308235164076 2212 9 0.46791912500495414 2212 10-11 0.5465407446202803 2212 12-13 0.5496133224783009 2212 14-15 0.7048898379743918 2212 16-17 0.6848763855450457 2212 18-19 0.6214482805182655 2212 20-21 0.6540323723456609 2212 22-23 0.6928171345703831 2212 24-25 0.6145135299384137 2212 26-27 0.5320850328073448 2212 28-29 0.530225327706539 2212 30-31 0.4943301997945184 2212 32-33 0.7448583691723343 2212 34-35 0.6516785454699772 2212 36-37 0.7016799125149404 2212 38-39 0.7357054059456232 2212 40-41 0.7977357426797411 2212 42-43 0.7495126946912833 2212 44-45 0.7944719208268722 2212 46-47 0.796305460261685 2212 48-49 0.777549063435309 2212 50-51 0.7965623084979541 2212 52-53 0.6742951301059392 2212 54-55 0.6326851208870679 2212 56-57 0.5691888436399068 2212 58-59 0.759891581858021 2212 60-61 0.6284120930768609 2212 62-63 0.7468497642655407 2212 64-65 0.5928148433641809 2212 66-67 0.6386896861252609 2212 68-69 0.8195732506720645 2212 70-71 0.8154213321039165 2212 72-73 0.7295086216600737 2212 74-75 0.7346059115976118 2212 76-77 0.9170629398762173 2212 78-79 0.7900921983661675 2212 80-81 0.7281645797637424 2212 82-83 0.8333231701526813 2212 84-85 0.983888559967582 2212 86-87 0.731918785529821 2212 88-89 0.8778233026466253 2212 90-91 0.7971893348360268 2212 92-93 0.7947294779196028 2212 94-95 0.8815589249289815 2212 96-97 0.7733697260733514 2212 98-99 0.943373325129226 2212 100 0.8037762264318964 2213 1 -0.48622044562515754 2213 2 -25.812683824352582 2213 3 -14.712399252434627 2213 4 -5.969387715852456 2213 5 -1.8774815732051024 2213 6 -1.064312319953899 2213 7 -0.9977870365865158 2213 8 -0.8563736206751216 2213 9 -0.9151912521005272 2213 10-11 -0.8512052150359111 2213 12-13 -0.9901275127261968 2213 14-15 -1.1881506020899693 2213 16-17 -1.2820009377775037 2213 18-19 -1.1808819586503958 2213 20-21 -1.1448443003809885 2213 22-23 -1.1795788926779593 2213 24-25 -1.1499316059996048 2213 26-27 -1.296187415122283 2213 28-29 -1.1967806779958536 2213 30-31 -1.280061042022325 2213 32-33 -1.1040530430344546 2213 34-35 -1.025092335601137 2213 36-37 -1.1395029491262747 2213 38-39 -1.2290333658770294 2213 40-41 -1.03714860804714 2213 42-43 -0.9864970214660786 2213 44-45 -1.0482048494488083 2213 46-47 -0.9664019953776162 2213 48-49 -1.110201148099165 2213 50-51 -1.1069011863926193 2213 52-53 -1.20726332151618 2213 54-55 -1.0751762327681078 2213 56-57 -0.9979238363813323 2213 58-59 -0.9495906112071211 2213 60-61 -1.0748241573839934 2213 62-63 -0.9503293253724152 2213 64-65 -0.8760059939470892 2213 66-67 -0.9755508369990409 2213 68-69 -1.137428495080151 2213 70-71 -1.2682496592900137 2213 72-73 -1.2332132826970792 2213 74-75 -1.2572905976446194 2213 76-77 -1.077385604425551 2213 78-79 -1.1180924554407383 2213 80-81 -1.2084692160698225 2213 82-83 -1.150378433957691 2213 84-85 -1.2082310568508063 2213 86-87 -1.0677687104972868 2213 88-89 -1.1477316562602908 2213 90-91 -1.1829276352032636 2213 92-93 -1.1421916778890342 2213 94-95 -1.1339066517070435 2213 96-97 -1.2299736588051822 2213 98-99 -1.087959304737204 2213 100 -1.1056567481656039 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 29.0 5 35.0 6 47.0 7 42.0 8 59.0 9 100.0 10 424.0 11 1942.0 12 5646.0 13 10036.0 14 13663.0 15 15433.0 16 16568.0 17 16805.0 18 17535.0 19 18265.0 20 20010.0 21 22362.0 22 24942.0 23 26525.0 24 27748.0 25 29028.0 26 31820.0 27 36695.0 28 42584.0 29 50857.0 30 60987.0 31 75795.0 32 95258.0 33 127568.0 34 174879.0 35 247681.0 36 375204.0 37 635229.0 38 657201.0 39 5001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.782664193820494 21.398231114289064 10.690264844059861 30.12883984783058 2 12.755937367184226 22.872282160221648 36.780019833822365 27.591760638771763 3 14.96317639960888 27.70941255608491 28.95978529968585 28.367625744620355 4 10.46812822223486 19.08419525442912 37.945044641432965 32.502631881903056 5 11.584954620625371 38.792506482023626 35.26540353158289 14.357135365768109 6 29.295678188467466 40.28625422530984 17.843496917254182 12.574570668968505 7 25.564268118148913 33.39884520582822 23.66065451286153 17.376232163161333 8 23.891245654707 37.55918461645732 21.017359664510945 17.532210064324733 9 24.346326328993083 17.151094220031748 22.267294393197687 36.23528505777748 10-11 22.802149199305664 28.141516358696634 29.270213698873437 19.78612074312427 12-13 23.479891834196412 25.605484119326494 29.746393822803658 21.168230223673437 14-15 21.098733102277684 27.08377634802795 26.79025056622048 25.02723998347389 16-17 20.445994529993982 30.800036073597077 27.58124797302805 21.172721423380896 18-19 20.367019825584247 29.64733070578428 29.666060100198795 20.31958936843268 20-21 21.694912138717697 28.865873010780792 28.799352552705237 20.639862297796277 22-23 20.828252370884595 28.889554789769544 29.193597501761865 21.088595337584 24-25 21.004230012523223 28.60639173377105 29.056617384148048 21.332760869557678 26-27 20.87799612018951 28.755800816050236 29.010911880307084 21.355291183453172 28-29 20.59194051844519 28.739476359643668 29.427506681669534 21.241076440241606 30-31 21.325990342989652 28.96766684216039 28.76324786858364 20.94309494626632 32-33 20.421812911323933 28.999455613423113 29.453567639165318 21.12516383608763 34-35 20.587957383648245 28.520659318673065 29.45194495055595 21.439438347122742 36-37 21.12081034583496 28.99112990738771 28.85974552902151 21.028314217755824 38-39 20.86148374813638 28.95165592664083 29.10535732150049 21.081503003722297 40-41 21.063532750492058 28.753843881206908 28.954967119818132 21.22765624848291 42-43 20.69854137650213 28.914197873243126 29.160541171562816 21.22671957869193 44-45 21.079069070677953 28.47752598297329 29.06002867433477 21.383376272013983 46-47 21.118140372366657 29.012169923932774 28.78284807848235 21.086841625218224 48-49 20.92888516161612 28.855299502090006 28.939475286932947 21.276340049360932 50-51 21.30909117393422 28.72336237881507 28.55732296253162 21.410223484719086 52-53 20.86124068353195 28.960173085400566 28.717855605627218 21.460730625440267 54-55 21.204971337415593 28.782031350660887 28.508246746726872 21.50475056519665 56-57 21.25555438800554 28.715436040049507 28.527350668329905 21.50165890361505 58-59 20.79828351929177 28.44499075713147 29.106582819344535 21.65014290423223 60-61 21.38230331058676 28.79746835443038 28.383306897003735 21.436921437979123 62-63 20.745756681003538 29.050530713992913 28.751464379425652 21.452248225577897 64-65 20.824216583796584 28.42509734070095 28.8690652691639 21.881620806338564 66-67 21.017931103845378 28.712621135309995 28.580918863944355 21.688528896900273 68-69 21.126212460798573 28.67595084049381 28.679401883888172 21.51843481481944 70-71 21.264326255635428 28.571334430373906 28.392696824357145 21.77164248963352 72-73 20.913005787313413 28.828114554573865 28.781050255272667 21.477829402840054 74-75 21.24858689754233 28.3279289046672 28.54077651399871 21.88270768379176 76-77 21.11655304498309 28.808337863461503 28.497445354516127 21.57766373703928 78-79 21.06676413745462 28.715244387309053 28.685732655615432 21.532258819620896 80-81 21.306647450260037 28.482491095477357 28.394876349289632 21.815985104972974 82-83 20.7622778175019 28.74973385607353 28.743440029017492 21.744548297407082 84-85 20.96543865416599 28.27932949657452 28.602925979406894 22.152305869852594 86-87 21.095883048013047 28.672985412211023 28.401099610836244 21.830031928939686 88-89 20.815213414813872 28.316642953716926 28.8177531220124 22.0503905094568 90-91 21.107289173745457 28.36951921438121 28.44327630595912 22.079915305914213 92-93 20.66098766027144 28.733520776491112 28.67838703642797 21.927104526809472 94-95 20.730960760187816 28.129456203017263 28.82238954164422 22.317193495150697 96-97 20.895610367378374 28.420813744576307 28.631840938339387 22.05173494970594 98-99 20.74838470761769 28.322575119677612 28.688854846639977 22.240185326064722 100 20.956913617229695 28.097075657601287 28.454847413314027 22.491163311854994 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19.0 1 12.0 2 8.5 3 15.5 4 18.0 5 23.0 6 49.0 7 86.0 8 148.5 9 176.0 10 164.5 11 187.5 12 225.5 13 272.5 14 342.5 15 428.0 16 527.5 17 649.5 18 833.5 19 1096.0 20 1506.0 21 2143.0 22 3053.0 23 4591.5 24 6785.5 25 10025.0 26 14880.5 27 21500.0 28 29864.0 29 39726.0 30 50441.5 31 61594.0 32 72853.0 33 83782.0 34 93735.5 35 102216.0 36 109890.5 37 116352.0 38 121375.0 39 125015.0 40 126231.5 41 125178.0 42 122070.0 43 117551.5 44 112190.0 45 106008.0 46 100317.0 47 94829.5 48 89161.0 49 85245.5 50 86117.0 51 80929.0 52 69249.0 53 62855.0 54 58656.5 55 53933.0 56 49326.5 57 44533.5 58 39685.5 59 35078.5 60 30080.5 61 25086.0 62 20669.5 63 16761.5 64 13328.5 65 10610.0 66 8248.5 67 6248.0 68 4659.5 69 3469.5 70 2570.5 71 1897.0 72 1464.0 73 1056.5 74 750.0 75 538.0 76 328.5 77 192.5 78 133.5 79 86.0 80 42.0 81 22.5 82 12.5 83 6.0 84 3.0 85 1.0 86 2.0 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.688067350434463 2 5.036858274214465 3 0.0 4 0.005270421148258334 5 0.1566563338673102 6 0.058494739980998744 7 0.03751707685799682 8 0.045179991816977697 9 0.04285684565294277 10-11 0.04410510329331974 12-13 0.042423422861145206 14-15 0.04538803475704052 16-17 0.035540668927399946 18-19 0.029524760577249815 20-21 0.02415031795896006 22-23 0.024549066927413817 24-25 0.020006796069375384 26-27 0.012968009930583004 28-29 0.01981609004098446 30-31 0.025173195747602305 32-33 0.001386952933752193 34-35 0.0035887407160837994 36-37 0.004004826596209458 38-39 0.006293298936900576 40-41 0.007489545842261842 42-43 0.008304380690841256 44-45 0.008893835687685939 46-47 0.0179610404920909 48-49 0.026629496328042106 50-51 0.023179450905333528 52-53 0.028397861318576155 54-55 0.02536390177599323 56-57 0.01695549961512056 58-59 0.01544718829966505 60-61 0.012551924050457347 62-63 0.018931907545717434 64-65 0.02049222959618865 66-67 0.021584455031518505 68-69 0.028917968668733224 70-71 0.028033786173466205 72-73 0.025329227952649424 74-75 0.025537270892712252 76-77 0.018983918280733144 78-79 0.014597679627741833 80-81 0.012933336107239202 82-83 0.008391065249200767 84-85 0.011650404643518422 86-87 0.009448616861186815 88-89 0.0077322626056684765 90-91 0.007957642457403208 92-93 0.004282217182959896 94-95 0.0016816804321745343 96-97 0.001092225435329852 98-99 7.628241135637062E-4 100 0.10863308853614052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2884020.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.4377459225555 #Duplication Level Percentage of deduplicated Percentage of total 1 91.59258564631601 80.08649232132484 2 6.54691026605754 11.44894152842619 3 1.0610534367135058 2.7832836242882957 4 0.32137241568818575 1.124003185178459 5 0.15394932552637752 0.6730491005162093 6 0.08886817622668317 0.4662259808105766 7 0.05950743026148208 0.3642236897395362 8 0.033622993415774696 0.23519350043554113 9 0.025239553106226713 0.1986200668530988 >10 0.11187552729968182 1.5796474614610783 >50 0.003065975225008773 0.18589022227903584 >100 0.0014332001096708173 0.29359007040889296 >500 2.381787943386509E-4 0.1427956844949996 >1k 2.778752594899766E-4 0.41804356378318963 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3591 0.12451369962760313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.467382334380483E-5 0.0 3 0.0 0.0 0.0 3.467382334380483E-5 0.0 4 0.0 0.0 0.0 3.467382334380483E-5 0.0 5 0.0 0.0 0.0 6.934764668760966E-5 0.0 6 0.0 0.0 0.0 6.934764668760966E-5 0.0 7 0.0 0.0 0.0 1.0402147003141448E-4 0.0 8 0.0 3.467382334380483E-5 0.0 1.0402147003141448E-4 0.0 9 0.0 3.467382334380483E-5 0.0 1.0402147003141448E-4 0.0 10-11 0.0 3.467382334380483E-5 0.0 1.386952933752193E-4 0.0 12-13 0.0 3.467382334380483E-5 0.0 1.386952933752193E-4 0.0 14-15 1.7336911671902414E-5 5.2010735015707246E-5 0.0 1.560322050471217E-4 0.0 16-17 3.467382334380483E-5 6.934764668760966E-5 0.0 1.7336911671902413E-4 0.0 18-19 3.467382334380483E-5 8.668455835951207E-5 0.0 1.7336911671902413E-4 0.0 20-21 3.467382334380483E-5 1.0402147003141448E-4 0.0 1.7336911671902413E-4 0.0 22-23 3.467382334380483E-5 1.0402147003141448E-4 0.0 1.7336911671902413E-4 0.0 24-25 3.467382334380483E-5 1.386952933752193E-4 0.0 2.0804294006282896E-4 0.0 26-27 3.467382334380483E-5 1.7336911671902413E-4 0.0 2.2537985173473138E-4 0.0 28-29 5.2010735015707246E-5 1.7336911671902413E-4 0.0 6.761395552041941E-4 0.0 30-31 6.934764668760966E-5 1.7336911671902413E-4 0.0 0.002253798517347314 0.0 32-33 6.934764668760966E-5 1.7336911671902413E-4 0.0 0.007385524372230428 0.0 34-35 6.934764668760966E-5 1.9070602839092656E-4 0.0 0.015620557416384075 0.0 36-37 6.934764668760966E-5 2.600536750785362E-4 0.0 0.027912427791762888 0.0 38-39 6.934764668760966E-5 3.120644100942435E-4 0.0 0.046809661514136514 0.0 40-41 6.934764668760966E-5 3.120644100942435E-4 0.0 0.07030117682956429 0.0 42-43 6.934764668760966E-5 3.120644100942435E-4 0.0 0.09459019008189956 0.0 44-45 6.934764668760966E-5 3.120644100942435E-4 0.0 0.12388957080741465 0.0 46-47 6.934764668760966E-5 3.120644100942435E-4 0.0 0.16001969473165928 0.0 48-49 6.934764668760966E-5 3.4673823343804827E-4 0.0 0.19793552055810987 0.0 50-51 6.934764668760966E-5 3.814120567818531E-4 0.0 0.23976948842241036 0.0 52-53 6.934764668760966E-5 3.814120567818531E-4 0.0 0.28283437701541597 0.0 54-55 6.934764668760966E-5 3.814120567818531E-4 0.0 0.3339956033592 0.0 56-57 6.934764668760966E-5 3.814120567818531E-4 0.0 0.39514289082599985 0.0 58-59 6.934764668760966E-5 3.814120567818531E-4 0.0 0.4638143979584053 0.0 60-61 6.934764668760966E-5 3.814120567818531E-4 0.0 0.5378430107974286 0.0 62-63 6.934764668760966E-5 4.1608588012565797E-4 0.0 0.6124610786332966 0.0 64-65 6.934764668760966E-5 4.5075970346946276E-4 0.0 0.6969785230338208 0.0 66-67 6.934764668760966E-5 4.680966151413652E-4 0.0 0.794134576043162 0.0 68-69 6.934764668760966E-5 4.854335268132676E-4 0.0 0.9056282550051664 0.0 70-71 6.934764668760966E-5 4.854335268132676E-4 0.0 1.0286683171406579 0.0 72-73 8.668455835951207E-5 5.374442618289748E-4 0.0 1.155661195137343 0.0 74-75 1.0402147003141448E-4 5.547811735008773E-4 0.0 1.2891554150109916 0.0 76-77 1.0402147003141448E-4 5.547811735008773E-4 0.0 1.4450835985880819 0.0 78-79 1.0402147003141448E-4 5.547811735008773E-4 0.0 1.6263583470294936 0.0 80-81 1.0402147003141448E-4 5.547811735008773E-4 0.0 1.817237744537139 0.0 82-83 1.0402147003141448E-4 5.721180851727797E-4 0.0 2.024812587984827 0.0 84-85 1.0402147003141448E-4 6.24128820188487E-4 0.0 2.2400850202148392 0.0 86-87 1.0402147003141448E-4 6.24128820188487E-4 0.0 2.4782075020284187 0.0 88 1.0402147003141448E-4 6.24128820188487E-4 0.0 2.6762990547915755 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7205 0.0 23.452372 1 TCAACGC 8005 0.0 20.792866 4 ATCAACG 8090 0.0 20.632519 3 CAACGCA 8040 0.0 20.526907 5 AACGCAG 8180 0.0 20.321913 6 TATCAAC 8320 0.0 19.932732 2 TACACCG 225 1.8402188E-6 18.807558 5 TAGGGCG 130 0.008023171 18.08419 5 ACGCAGA 9245 0.0 18.029797 7 CGCAGAG 9290 0.0 17.84138 8 GAGTACT 3465 0.0 16.463411 12-13 GTACATG 9600 0.0 16.259445 1 GGTATCA 5360 0.0 15.993656 1 ACATGGG 9315 0.0 15.647727 3 TACATGG 9760 0.0 15.622344 2 GCAGAGT 10575 0.0 15.45088 9 GTACTAT 1140 0.0 15.213517 1 GTATTAG 1425 0.0 14.257238 1 CATGGGG 4085 0.0 14.157464 4 TATATCG 200 0.005649374 14.105669 5 >>END_MODULE