FastQCFastQC Report
Wed 25 May 2016
SRR1295112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983125
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACC2101.1494474E-519.2484058
GTACCGT1700.001188440717.8194266
GTATACG2502.0547287E-417.2814941
GTACATG109500.016.4585651
GTATAGC6800.016.3375451
TGTACCG2807.7242075E-616.1798235
TACATGG112100.015.5231952
GTATATA21850.015.2533631
GTATAGG8950.015.1713031
TATACTG14050.015.0474275
TAGACCG2356.058521E-414.9940685
TACCGTG2701.0420123E-414.8851277
ACATGGG110000.014.7511113
GTCCTAC5902.5993359E-914.6453331
CATGGGG34000.014.1530044
GTGTAGC8850.013.9479371
ATTATAC14550.013.8439143
GTACATA15200.013.8057041
GGATACG2700.00680226313.715471
GTCTCAC16750.013.6335881
Produced by FastQC (version 0.11.5)