##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295112_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2983125
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.93953521893987	27.0	22.0	33.0	6.0	33.0
2	22.0258597946784	27.0	2.0	33.0	2.0	33.0
3	24.49924994762204	27.0	15.0	33.0	15.0	33.0
4	24.51519865912424	33.0	6.0	37.0	2.0	37.0
5	26.68023767022837	33.0	15.0	37.0	2.0	37.0
6	27.494956463440186	33.0	15.0	37.0	2.0	37.0
7	27.050263063063063	33.0	15.0	37.0	6.0	37.0
8	27.782359773727215	33.0	15.0	37.0	6.0	37.0
9	28.827656903415043	33.0	22.0	37.0	15.0	37.0
10-11	28.407158432851457	33.0	18.5	37.0	10.5	37.0
12-13	27.217248690551017	33.0	15.0	37.0	4.0	37.0
14-15	28.92954921433061	33.0	22.0	37.0	6.0	40.0
16-17	29.35677619945527	33.0	24.5	37.0	6.0	40.0
18-19	29.527870940708148	33.0	24.5	38.5	10.5	40.0
20-21	29.04270504923528	33.0	22.0	37.0	6.0	40.0
22-23	29.825505971087367	33.0	27.0	37.0	10.5	40.0
24-25	29.977613743976534	33.0	27.0	37.0	15.0	40.0
26-27	28.564912801173264	33.0	18.5	37.0	4.0	40.0
28-29	29.268396228786926	33.0	22.0	37.0	6.0	40.0
30-31	30.013143264194426	33.0	27.0	37.0	15.0	40.0
32-33	28.811266373350094	33.0	22.0	37.0	6.0	40.0
34-35	28.336517242824222	33.0	22.0	37.0	6.0	40.0
36-37	28.60792357008171	33.0	22.0	37.0	6.0	40.0
38-39	28.75914737062644	33.0	22.0	37.0	6.0	40.0
40-41	27.87096953697884	33.0	22.0	37.0	6.0	40.0
42-43	28.475110831761995	33.0	22.0	37.0	6.0	40.0
44-45	28.277985166561912	33.0	22.0	37.0	6.0	40.0
46-47	28.06977582233396	33.0	22.0	37.0	6.0	40.0
48-49	27.934154452126545	33.0	22.0	37.0	6.0	40.0
50-51	27.777224890006288	33.0	22.0	37.0	6.0	40.0
52-53	28.051585166561914	33.0	22.0	37.0	6.0	40.0
54-55	28.055708527131785	33.0	22.0	37.0	6.0	40.0
56-57	28.019461805992037	33.0	22.0	37.0	6.0	40.0
58-59	27.864714351560863	33.0	22.0	37.0	6.0	40.0
60-61	27.82974783155248	33.0	22.0	37.0	6.0	40.0
62-63	27.770465367693276	33.0	22.0	37.0	6.0	40.0
64-65	27.372653676932746	33.0	22.0	37.0	6.0	40.0
66-67	27.63432440812906	33.0	22.0	37.0	6.0	38.5
68-69	27.132759270898806	33.0	22.0	37.0	6.0	37.0
70-71	27.281803813115438	33.0	22.0	37.0	6.0	37.0
72-73	27.310692813743977	33.0	22.0	37.0	10.5	37.0
74-75	27.432033857112927	30.0	22.0	37.0	10.5	37.0
76-77	27.218286360779384	27.0	22.0	37.0	10.5	37.0
78-79	26.76107320343599	27.0	22.0	33.0	6.0	37.0
80-81	26.591776911795513	27.0	22.0	33.0	6.0	37.0
82-83	26.670187974020532	27.0	22.0	33.0	10.5	37.0
84-85	26.61184931908653	27.0	22.0	33.0	10.5	37.0
86-87	26.4102154619736	27.0	22.0	33.0	10.5	37.0
88-89	26.350723318667505	27.0	22.0	33.0	10.5	37.0
90-91	26.474076513723027	27.0	22.0	33.0	15.0	37.0
92-93	26.390587722606327	27.0	22.0	33.0	10.5	37.0
94-95	26.2652220406453	27.0	22.0	33.0	10.5	37.0
96-97	26.351518290383407	27.0	22.0	33.0	10.5	37.0
98-99	26.416224596689716	27.0	22.0	33.0	15.0	37.0
100	26.052406788183532	27.0	22.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.278356013646132
1101	2	11.294773675737972
1101	3	8.523465273116717
1101	4	12.849640792717668
1101	5	10.927757202813485
1101	6	10.12372070224059
1101	7	9.676160262765425
1101	8	9.312936087004822
1101	9	7.692463747743179
1101	10-11	8.006698136259164
1101	12-13	9.387268964424969
1101	14-15	8.245504542750677
1101	16-17	8.417662617947993
1101	18-19	8.47038011444786
1101	20-21	7.570420147382698
1101	22-23	7.61243873302006
1101	24-25	7.605833721024869
1101	26-27	8.764027465888741
1101	28-29	8.455093532914326
1101	30-31	5.750700125047469
1101	32-33	7.210508643278558
1101	34-35	8.893704775146617
1101	36-37	8.525261553572516
1101	38-39	8.380957805174546
1101	40-41	9.213412679979658
1101	42-43	8.805117144397261
1101	44-45	8.859219270615874
1101	46-47	7.5748309988589675
1101	48-49	5.653654047031434
1101	50-51	7.021679669410787
1101	52-53	6.071001353003421
1101	54-55	4.309006920504597
1101	56-57	6.477420429973922
1101	58-59	6.487371726273654
1101	60-61	6.937729003662408
1101	62-63	6.563421272766281
1101	64-65	7.03341091077575
1101	66-67	6.022657937258312
1101	68-69	6.9792132557226765
1101	70-71	6.504504897267825
1101	72-73	5.797487022150584
1101	74-75	5.154317616762377
1101	76-77	5.305069881302931
1101	78-79	5.388977580680503
1101	80-81	5.2018096843824395
1101	82-83	4.761542217497631
1101	84-85	4.49360626349964
1101	86-87	4.405526465750551
1101	88-89	4.361642696365763
1101	90-91	4.117140770314869
1101	92-93	4.027668949778725
1101	94-95	3.9688733547398165
1101	96-97	4.12661453865811
1101	98-99	3.795573866638893
1101	100	3.9927168337234207
1102	1	6.701505512021001
1102	2	10.378209333377043
1102	3	8.11882608403523
1102	4	12.5018695304264
1102	5	10.590992268742209
1102	6	9.51579773806926
1102	7	6.725723226865238
1102	8	7.899678743250014
1102	9	6.593912714489608
1102	10-11	7.598451045009281
1102	12-13	9.240842294794824
1102	14-15	6.2911773796772685
1102	16-17	7.55575145241448
1102	18-19	7.486011785151195
1102	20-21	7.043211017454368
1102	22-23	6.592042524588432
1102	24-25	7.063324923518412
1102	26-27	8.451482433862775
1102	28-29	7.7269909657089215
1102	30-31	4.470477278094673
1102	32-33	4.49161258178178
1102	34-35	5.1489767156530135
1102	36-37	5.133077010177619
1102	38-39	4.531685459668097
1102	40-41	6.301859201764081
1102	42-43	7.0321840396982545
1102	44-45	6.508516698084488
1102	46-47	5.530861039437077
1102	48-49	3.595520142193841
1102	50-51	5.007101808102792
1102	52-53	5.88950778543753
1102	54-55	5.4638598073391655
1102	56-57	6.219521917304636
1102	58-59	5.903844275522239
1102	60-61	6.030737727819069
1102	62-63	4.3041008607916424
1102	64-65	6.094309053920124
1102	66-67	3.749626217303806
1102	68-69	6.082315011328291
1102	70-71	5.192399775852028
1102	72-73	5.038538558125531
1102	74-75	4.111527182366881
1102	76-77	3.3024922638983156
1102	78-79	4.419590983566447
1102	80-81	4.448214885433821
1102	82-83	4.003472257313543
1102	84-85	3.031248379327586
1102	86-87	3.148145353827786
1102	88-89	3.4306824792465918
1102	90-91	3.380443149624334
1102	92-93	3.272300417135149
1102	94-95	3.053610677292344
1102	96-97	3.2936518202119096
1102	98-99	3.0429556927694676
1102	100	3.2309285702750223
1103	1	6.625222403289655
1103	2	10.889632087265728
1103	3	7.935198464122774
1103	4	11.874577633419623
1103	5	10.359346555967118
1103	6	9.210377991386515
1103	7	7.990251557923205
1103	8	8.186595727282999
1103	9	7.234904958837735
1103	10-11	7.480895036872358
1103	12-13	8.868411267988122
1103	14-15	8.7027944423977
1103	16-17	8.2233622411356
1103	18-19	7.356966742436175
1103	20-21	8.496897683965152
1103	22-23	6.959436505309295
1103	24-25	5.220847662471268
1103	26-27	6.008839900556616
1103	28-29	7.220597957009403
1103	30-31	6.234968007148854
1103	32-33	8.049632807651484
1103	34-35	7.4074899190621935
1103	36-37	4.218165477480813
1103	38-39	6.414125657927997
1103	40-41	7.026576005708282
1103	42-43	7.6665737205249975
1103	44-45	5.754361104046794
1103	46-47	6.392283268424691
1103	48-49	5.611322384284893
1103	50-51	6.515780546221539
1103	52-53	6.1741885716248035
1103	54-55	4.908603233311172
1103	56-57	5.923807925398609
1103	58-59	5.61780089388051
1103	60-61	5.192466928494952
1103	62-63	2.7614253541547455
1103	64-65	5.173474633007686
1103	66-67	4.187719480771218
1103	68-69	5.304771090231419
1103	70-71	3.8467711042287043
1103	72-73	4.584016296832644
1103	74-75	3.3566763568645186
1103	76-77	3.6880753311624765
1103	78-79	2.9484588005935706
1103	80-81	4.218939267995083
1103	82-83	3.494729266532236
1103	84-85	3.2938193304435472
1103	86-87	2.7723937551461475
1103	88-89	3.017806775342329
1103	90-91	2.6999366391809545
1103	92-93	2.353175837592829
1103	94-95	2.774653531647086
1103	96-97	2.9446137491374387
1103	98-99	2.7910725624694592
1103	100	2.971027862469793
1104	1	6.285356276948214
1104	2	9.582637466170254
1104	3	7.409563499198143
1104	4	12.166346244107896
1104	5	9.626964050895477
1104	6	8.396654918778609
1104	7	4.177770707427143
1104	8	6.742540181304225
1104	9	6.943876811866939
1104	10-11	7.266587496569532
1104	12-13	7.560416636179639
1104	14-15	7.318451943598038
1104	16-17	7.235088141004024
1104	18-19	7.066887938514437
1104	20-21	7.715604984101795
1104	22-23	6.559947321065987
1104	24-25	6.142432038618654
1104	26-27	8.333873841317565
1104	28-29	7.254299574381612
1104	30-31	6.515080936874437
1104	32-33	7.802001916592076
1104	34-35	8.187560824574426
1104	36-37	6.685611466871034
1104	38-39	7.379205362716419
1104	40-41	7.273667326320403
1104	42-43	7.3681197781863155
1104	44-45	6.818943798251738
1104	46-47	6.825742041356619
1104	48-49	5.636975439586422
1104	50-51	5.8343837597347346
1104	52-53	6.0562140169694665
1104	54-55	4.994375237101632
1104	56-57	6.097337004888164
1104	58-59	3.970202020109948
1104	60-61	4.3047498317983575
1104	62-63	3.9295268092735682
1104	64-65	2.937136884940653
1104	66-67	3.353167566989516
1104	68-69	4.344244122178587
1104	70-71	2.6829228598474337
1104	72-73	2.5323225457309064
1104	74-75	3.356899163108057
1104	76-77	4.0050347551531935
1104	78-79	4.4911040763026016
1104	80-81	3.2312425747114553
1104	82-83	3.0642053711652224
1104	84-85	3.1994790618976268
1104	86-87	0.90134183990358
1104	88-89	0.4974603117553613
1104	90-91	2.405017308140202
1104	92-93	2.6623507508418065
1104	94-95	2.6627226967822786
1104	96-97	2.9493323183858458
1104	98-99	2.7258695850320294
1104	100	0.8464672175855945
1105	1	5.761342223250786
1105	2	-2.6753822305300403
1105	3	0.5634619555607898
1105	4	8.078832323765013
1105	5	8.538577574606077
1105	6	8.045057471699309
1105	7	5.241715186704937
1105	8	5.85897137191856
1105	9	4.954120741505648
1105	10-11	5.015235868318332
1105	12-13	4.986514432632255
1105	14-15	5.773585206510816
1105	16-17	6.022339193023214
1105	18-19	6.160828075971001
1105	20-21	7.073763660023737
1105	22-23	2.1012385868891705
1105	24-25	4.139040612224125
1105	26-27	6.953173324432232
1105	28-29	6.005396776197735
1105	30-31	5.427925650575485
1105	32-33	7.203352063126104
1105	34-35	6.149288557484773
1105	36-37	6.273920786638083
1105	38-39	4.14414355756379
1105	40-41	5.478913813870861
1105	42-43	5.571246874036259
1105	44-45	6.25007738597251
1105	46-47	5.409624682338109
1105	48-49	5.065070093628854
1105	50-51	6.064286215953892
1105	52-53	5.738312426775003
1105	54-55	5.371131998021109
1105	56-57	5.237046410906743
1105	58-59	1.9590962574779205
1105	60-61	4.488280097041329
1105	62-63	-0.7835682951329055
1105	64-65	-0.3415894192823963
1105	66-67	0.8659722840751165
1105	68-69	2.8214098370369314
1105	70-71	3.0195570106207335
1105	72-73	2.6568548787491792
1105	74-75	2.766841465821315
1105	76-77	1.8407447823232062
1105	78-79	3.2925768836731315
1105	80-81	1.7690536573320053
1105	82-83	1.135241670506403
1105	84-85	1.4359644365693072
1105	86-87	-0.29056480645576244
1105	88-89	1.5519061553827278
1105	90-91	1.3928526538794088
1105	92-93	1.6410677358437056
1105	94-95	0.9876310545249574
1105	96-97	2.038897247232228
1105	98-99	1.9996471001299483
1105	100	2.080638383993353
1106	1	6.123199361350149
1106	2	10.294441598531648
1106	3	6.836332107658741
1106	4	11.744234538089447
1106	5	10.02895675335677
1106	6	9.51183877711101
1106	7	6.692587973682059
1106	8	7.620685444462531
1106	9	7.07849819722988
1106	10-11	5.778140024444973
1106	12-13	7.987796653724764
1106	14-15	7.933111124450196
1106	16-17	7.861993751291447
1106	18-19	7.1995700685490895
1106	20-21	8.27755668687707
1106	22-23	6.271026221293656
1106	24-25	5.08466859526872
1106	26-27	7.792907459266996
1106	28-29	6.343052804646625
1106	30-31	5.443007826419883
1106	32-33	7.402621906258339
1106	34-35	6.76927839197317
1106	36-37	6.15129284302289
1106	38-39	7.1191556304247285
1106	40-41	6.731742681856641
1106	42-43	6.187226083067198
1106	44-45	4.60674898386614
1106	46-47	6.190906311989625
1106	48-49	5.969763996121102
1106	50-51	6.698097346834999
1106	52-53	5.7712858599731796
1106	54-55	5.257818197233746
1106	56-57	4.954071207213701
1106	58-59	5.0835069697949
1106	60-61	5.03488741735811
1106	62-63	5.164808285488352
1106	64-65	5.418126243070258
1106	66-67	3.1717875414533907
1106	68-69	3.49361044061299
1106	70-71	3.012214768412445
1106	72-73	2.99223658691497
1106	74-75	2.9275143055496144
1106	76-77	3.6350910371427787
1106	78-79	4.29709054500292
1106	80-81	3.7322591373294323
1106	82-83	2.200061999730405
1106	84-85	2.8687060162871347
1106	86-87	3.504637889366709
1106	88-89	3.451142005013544
1106	90-91	3.300824268731507
1106	92-93	3.2132833981963635
1106	94-95	1.6510002383057696
1106	96-97	1.716801452167104
1106	98-99	2.072490833573429
1106	100	2.977628791547872
1107	1	5.887430239122825
1107	2	10.141241683929525
1107	3	5.912753792319812
1107	4	11.027469159155093
1107	5	9.24205918213649
1107	6	7.853227903469843
1107	7	3.5539512519496483
1107	8	6.016221161193744
1107	9	5.4579218153562366
1107	10-11	4.294687990044213
1107	12-13	5.796258667308944
1107	14-15	5.854573725565263
1107	16-17	6.660557921345092
1107	18-19	5.964645494934228
1107	20-21	5.236526265732223
1107	22-23	5.5897918889239655
1107	24-25	4.333570320481769
1107	26-27	6.928203250816974
1107	28-29	6.160891202276396
1107	30-31	4.870439521690347
1107	32-33	4.669219101054264
1107	34-35	6.297450334348724
1107	36-37	6.227908020771043
1107	38-39	6.172840333685592
1107	40-41	7.476671353820134
1107	42-43	5.947246386927386
1107	44-45	3.8012315802038827
1107	46-47	3.1989512733104704
1107	48-49	3.7286345735961532
1107	50-51	4.608404993996057
1107	52-53	3.7033918182148042
1107	54-55	5.029711302748421
1107	56-57	5.045798703212057
1107	58-59	2.85269024709088
1107	60-61	2.5294251028247245
1107	62-63	0.2809819458163112
1107	64-65	1.7548312918523337
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2207	12-13	8.940938121494504
2207	14-15	8.26573230306698
2207	16-17	7.362909821733819
2207	18-19	4.235004305650872
2207	20-21	7.5249713597644
2207	22-23	6.368119985398362
2207	24-25	4.438392734864923
2207	26-27	3.9766134071392365
2207	28-29	5.191588924032544
2207	30-31	5.709106330247739
2207	32-33	6.873194000985656
2207	34-35	6.267496792824616
2207	36-37	6.759690955527784
2207	38-39	6.861300593414313
2207	40-41	4.156737318797202
2207	42-43	5.676068396481419
2207	44-45	3.953283853278414
2207	46-47	5.55258600617098
2207	48-49	2.3761940061087223
2207	50-51	5.426777705993274
2207	52-53	2.7805895125078557
2207	54-55	3.1110109117538833
2207	56-57	5.296302519114949
2207	58-59	3.698596810531015
2207	60-61	3.566186687298231
2207	62-63	4.184702948823325
2207	64-65	3.5184151479813863
2207	66-67	3.523057064510585
2207	68-69	3.4962203842745723
2207	70-71	3.643423520661358
2207	72-73	3.8676280053690917
2207	74-75	3.498432048219623
2207	76-77	4.0322430250446395
2207	78-79	4.238041645454615
2207	80-81	4.271701179669236
2207	82-83	3.9731882806928454
2207	84-85	3.3430897737599885
2207	86-87	3.434367345005686
2207	88-89	3.188329798676122
2207	90-91	1.353686916358292
2207	92-93	-0.8972067790696698
2207	94-95	1.3459774885118172
2207	96-97	-1.1336735741318122
2207	98-99	0.6177840220941739
2207	100	2.1556725087467328
2208	1	1.065566550798554
2208	2	8.395353736075094
2208	3	6.537015988476188
2208	4	11.68935467291276
2208	5	9.955346252835884
2208	6	9.202286632872081
2208	7	7.768611028528831
2208	8	7.932898415046601
2208	9	6.727300715374707
2208	10-11	6.780150470780395
2208	12-13	6.951472149650929
2208	14-15	7.876053449324811
2208	16-17	7.307653798182276
2208	18-19	6.617760904166044
2208	20-21	6.778931825523699
2208	22-23	5.957923328456548
2208	24-25	6.217884250598257
2208	26-27	7.95527773432843
2208	28-29	7.0755298507188655
2208	30-31	6.137752697898865
2208	32-33	8.324564837021533
2208	34-35	6.464589318039035
2208	36-37	6.843505470969102
2208	38-39	7.215166642393463
2208	40-41	7.663082268549257
2208	42-43	6.9864788236083335
2208	44-45	5.8496802525848395
2208	46-47	4.467694483718972
2208	48-49	4.641425374057022
2208	50-51	5.947993783139182
2208	52-53	5.715513966163201
2208	54-55	6.376963326084304
2208	56-57	6.534765534741037
2208	58-59	5.706547217010918
2208	60-61	5.238753945122262
2208	62-63	3.358019758950956
2208	64-65	3.223278322788417
2208	66-67	2.815110404984665
2208	68-69	5.380101634258423
2208	70-71	5.314397273248691
2208	72-73	4.669812657199543
2208	74-75	2.286069958737194
2208	76-77	3.4248833452412413
2208	78-79	4.150528466513013
2208	80-81	3.638112014438903
2208	82-83	1.3823261001206717
2208	84-85	2.013780405212181
2208	86-87	1.212038923196861
2208	88-89	0.7554546219878624
2208	90-91	1.9327287491614555
2208	92-93	0.8455389888104108
2208	94-95	-0.892688217944059
2208	96-97	1.3885731576198772
2208	98-99	0.2667545141855179
2208	100	1.7183015525499172
2209	1	5.215894339646635
2209	2	9.42295186274771
2209	3	5.45631201359291
2209	4	-3.2846604399850463
2209	5	0.549893097605743
2209	6	4.3220142792221665
2209	7	2.1813780674666994
2209	8	5.079700681691644
2209	9	4.906596581194879
2209	10-11	4.933983869763214
2209	12-13	7.028316803658832
2209	14-15	6.569621923669377
2209	16-17	6.14952427995296
2209	18-19	5.257066277093745
2209	20-21	5.869334787860204
2209	22-23	5.325273654869651
2209	24-25	5.098254173382873
2209	26-27	6.834265216516176
2209	28-29	6.119077182067265
2209	30-31	3.8932951316025317
2209	32-33	6.053176560378461
2209	34-35	5.436546066130376
2209	36-37	6.162127164841692
2209	38-39	6.7085040643381575
2209	40-41	7.038594370742338
2209	42-43	6.646488403123374
2209	44-45	6.396117730138506
2209	46-47	6.6905615753695145
2209	48-49	5.082950870193354
2209	50-51	4.514189100979234
2209	52-53	4.77174492298515
2209	54-55	5.345945392189474
2209	56-57	4.998353421542017
2209	58-59	3.0392013074566044
2209	60-61	4.147916537659075
2209	62-63	4.185515537592476
2209	64-65	4.9863867993921005
2209	66-67	4.438858478944606
2209	68-69	3.700192657753213
2209	70-71	3.6534246737028333
2209	72-73	0.7671643883185553
2209	74-75	2.3672429533384864
2209	76-77	3.4390107766858016
2209	78-79	3.385393484412152
2209	80-81	3.598479938775938
2209	82-83	2.891202176096435
2209	84-85	2.9653439370193553
2209	86-87	1.4113536584170383
2209	88-89	1.967188514210715
2209	90-91	2.353719834347455
2209	92-93	2.3527162033220357
2209	94-95	0.7456537729152153
2209	96-97	0.014346107907769579
2209	98-99	0.20484282529457332
2209	100	0.11985447665524873
2210	1	1.4160155893201463
2210	2	-16.734942874094397
2210	3	-7.512983182391817
2210	4	-9.032337459605046
2210	5	-2.3802745769933082
2210	6	2.0560364640196553
2210	7	-9.52267729370987
2210	8	-9.58981801624341
2210	9	-4.436664282182683
2210	10-11	-1.8759553086690417
2210	12-13	2.991313349086589
2210	14-15	-6.220773765135448
2210	16-17	-2.193110198534118
2210	18-19	-0.8824506909241343
2210	20-21	1.8212425522403421
2210	22-23	1.825850378639732
2210	24-25	1.2946217330049201
2210	26-27	3.479498071858984
2210	28-29	2.9378286530649333
2210	30-31	2.2151948365897667
2210	32-33	4.218949670708547
2210	34-35	1.8274232585148624
2210	36-37	3.5699286488032733
2210	38-39	2.4278611797571727
2210	40-41	3.536561628367366
2210	42-43	1.5271806408720003
2210	44-45	2.7101852097047967
2210	46-47	2.3657688186098618
2210	48-49	2.3230803263595377
2210	50-51	1.725262436551354
2210	52-53	1.8238254757741146
2210	54-55	2.2304734805909305
2210	56-57	2.3252734591852047
2210	58-59	1.1290391562743984
2210	60-61	2.2058894526454544
2210	62-63	1.1454013564813295
2210	64-65	2.08739331758607
2210	66-67	2.19722328325647
2210	68-69	2.707078974867784
2210	70-71	1.9058631961658037
2210	72-73	0.6939677686711931
2210	74-75	0.061014530960761704
2210	76-77	-0.5367136059496325
2210	78-79	-0.08826133605915842
2210	80-81	0.3490131604808795
2210	82-83	0.41632266037278853
2210	84-85	-1.6122379897771033
2210	86-87	-1.375004261386561
2210	88-89	-1.8166723721048434
2210	90-91	-2.1124710183910906
2210	92-93	-1.5448640443844042
2210	94-95	-0.6968263763898541
2210	96-97	-0.310705418868217
2210	98-99	-0.5424534989334084
2210	100	-0.46087169270418116
2211	1	0.8899738509164834
2211	2	-16.734942874094397
2211	3	-7.829896155439611
2211	4	-18.6758087653439
2211	5	-21.065537524403826
2211	6	-13.758896152778918
2211	7	-11.816168815239466
2211	8	-18.154507813994655
2211	9	-15.941625478512055
2211	10-11	-10.502681599952066
2211	12-13	-22.118064775288538
2211	14-15	-17.570597425220235
2211	16-17	-15.345284868631852
2211	18-19	-18.819822376333025
2211	20-21	-8.964943786456743
2211	22-23	-4.564249984287198
2211	24-25	-2.195885612521579
2211	26-27	-17.117487465083457
2211	28-29	-4.04855968236031
2211	30-31	-5.337047133189213
2211	32-33	-1.299673033670949
2211	34-35	-6.5847880705920545
2211	36-37	-7.680999621820082
2211	38-39	-3.2147364609491724
2211	40-41	-6.301100980781538
2211	42-43	-2.3386347158417102
2211	44-45	-0.4033627939333613
2211	46-47	0.5200507858607715
2211	48-49	0.5252150337609969
2211	50-51	-5.282070684025314
2211	52-53	-1.3653440425449865
2211	54-55	-0.7462012870446948
2211	56-57	-7.550707499972255
2211	58-59	-2.7883399961315654
2211	60-61	-1.4522611351168173
2211	62-63	-0.6119805574631911
2211	64-65	-4.103056257374362
2211	66-67	-3.1212585992629727
2211	68-69	-0.029148718528567485
2211	70-71	-0.334790407358728
2211	72-73	-1.2150498904252203
2211	74-75	-1.8346581176067396
2211	76-77	-1.768638984430364
2211	78-79	-1.1338083087359472
2211	80-81	-0.9449573077605962
2211	82-83	-1.5781131862146118
2211	84-85	-1.722892312258967
2211	86-87	-2.13217398034946
2211	88-89	-2.6460468001015123
2211	90-91	-2.312859860993843
2211	92-93	-2.1801511784573435
2211	94-95	-2.2930220126900487
2211	96-97	-3.0956601768174536
2211	98-99	-2.857334538358156
2211	100	-2.314054011785064
2212	1	-20.85453030150169
2212	2	-16.734942874094397
2212	3	-19.74733631176594
2212	4	-18.6758087653439
2212	5	-21.065537524403826
2212	6	-21.850982132261088
2212	7	-22.08656451626536
2212	8	-22.519432093427973
2212	9	-23.93356490205871
2212	10-11	-11.296280485777796
2212	12-13	-22.118064775288538
2212	14-15	-23.479122095469382
2212	16-17	-23.874473925788667
2212	18-19	-18.576451631955894
2212	20-21	-23.56338052314458
2212	22-23	-18.656023302076992
2212	24-25	-25.145690204607558
2212	26-27	-17.26523505175659
2212	28-29	-18.126076680470945
2212	30-31	-6.3576105653612665
2212	32-33	-17.305704732976334
2212	34-35	-13.954229653047003
2212	36-37	-17.568850619372668
2212	38-39	-23.641824883030267
2212	40-41	-10.318362765681734
2212	42-43	-14.12585695863213
2212	44-45	-17.117091479134885
2212	46-47	-17.03480873685871
2212	48-49	-17.70248831222328
2212	50-51	-9.192164983694724
2212	52-53	-2.5257992388393404
2212	54-55	-23.472385947523446
2212	56-57	-23.371787128697218
2212	58-59	-12.61404679215514
2212	60-61	-6.675755475300566
2212	62-63	-3.5981336703395534
2212	64-65	-17.359842558713716
2212	66-67	-17.67498268220478
2212	68-69	-14.734845948835092
2212	70-71	-9.87872535747282
2212	72-73	-17.595980341521923
2212	74-75	-6.537109836771975
2212	76-77	-11.373919362580194
2212	78-79	-5.349198427753574
2212	80-81	-3.0257379521431744
2212	82-83	-3.618255636537146
2212	84-85	-5.879731995597012
2212	86-87	-5.511925121207202
2212	88-89	-3.8809456542321534
2212	90-91	-4.732421150360999
2212	92-93	-6.054519044992418
2212	94-95	-4.500611090796323
2212	96-97	-6.172963466334508
2212	98-99	-4.350844870566657
2212	100	-4.514377072958997
2213	1	-20.85453030150169
2213	2	-16.734942874094397
2213	3	-19.74733631176594
2213	4	-18.6758087653439
2213	5	-21.065537524403826
2213	6	-21.850982132261088
2213	7	-10.394226128396244
2213	8	-10.218953242669793
2213	9	-4.120715740047537
2213	10-11	-17.52061540298697
2213	12-13	-16.09511984312573
2213	14-15	-23.479122095469382
2213	16-17	-17.85152899362586
2213	18-19	-18.47597472354778
2213	20-21	-17.626429206304994
2213	22-23	-19.830087986759814
2213	24-25	-7.538547347464704
2213	26-27	-23.18636875477569
2213	28-29	-24.10266324855474
2213	30-31	-19.380833204410173
2213	32-33	-23.307245090339244
2213	34-35	-17.85354461814125
2213	36-37	-23.68222092128271
2213	38-39	-11.51592703785868
2213	40-41	-17.07168479896329
2213	42-43	-23.21797032893404
2213	44-45	-17.07917016742409
2213	46-47	-16.953674315907687
2213	48-49	-6.097851209599831
2213	50-51	-23.32432764543225
2213	52-53	-17.77050940824669
2213	54-55	-17.630007655424482
2213	56-57	-17.413686570038
2213	58-59	-18.00742357512186
2213	60-61	-19.284962017922364
2213	62-63	-17.531245157791236
2213	64-65	-17.60244122149175
2213	66-67	-8.627434690176777
2213	68-69	-22.704038801576928
2213	70-71	-17.369348601059748
2213	72-73	-9.368527952455928
2213	74-75	-4.25332659123778
2213	76-77	-3.1814686134477377
2213	78-79	-4.595801527940974
2213	80-81	-3.2243414024453187
2213	82-83	-3.2020705005800174
2213	84-85	-3.0113064203048836
2213	86-87	-3.2492117024203004
2213	88-89	-2.801193743967744
2213	90-91	-2.7616124590574636
2213	92-93	-3.7394468756234254
2213	94-95	-3.7115056831462176
2213	96-97	-3.2169997386155984
2213	98-99	-3.694487657145661
2213	100	-3.0810579507438725
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	828.0
3	798.0
4	4262.0
5	15743.0
6	22348.0
7	26023.0
8	23623.0
9	24082.0
10	29006.0
11	35749.0
12	45756.0
13	59176.0
14	49603.0
15	55506.0
16	62637.0
17	65249.0
18	65544.0
19	64238.0
20	63443.0
21	61832.0
22	64636.0
23	70250.0
24	76326.0
25	84443.0
26	91485.0
27	98060.0
28	105042.0
29	110005.0
30	116737.0
31	128085.0
32	143974.0
33	172409.0
34	204654.0
35	232975.0
36	245475.0
37	202833.0
38	60166.0
39	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.17036458881551	22.020031023720872	14.136225384163478	23.67337900330014
2	14.96718214637054	21.725531147269137	40.531584242948476	22.775702463411847
3	17.426823229948894	27.333675346288118	30.021503915571657	25.21799750819133
4	12.304616334518299	18.38689973805306	39.88947084598129	29.41901308144735
5	12.777654650985475	37.91563454619412	36.60802801196408	12.698682790856319
6	29.20510052679936	39.64700444747344	18.806053466081245	12.341841559645951
7	26.080467312096417	32.23225235730513	24.59858643939546	17.08869389120299
8	23.26085350083133	36.64548309624977	22.447182708294847	17.646480694624053
9	24.14215947471699	17.4800326241558	22.818472717765527	35.55933518336168
10-11	22.69170815893607	27.94188793925002	29.665436538675337	19.700967363138577
12-13	23.58991995003496	25.591290695527043	29.593059230387965	21.225730124050035
14-15	21.06705195678669	27.101216009901236	26.98070087260441	24.85103116070767
16-17	20.576131011603177	30.74283981791296	27.895604598073543	20.785424572410317
18-19	20.59575979654279	29.78804381175277	29.409215785316867	20.206980606387567
20-21	21.985876258430263	29.153002215975153	28.273742226216196	20.587379299378387
22-23	20.832584969390286	29.276086366593173	28.847002637916212	21.044326026100325
24-25	21.229606710058228	29.057613011525575	28.34116418689637	21.37161609151983
26-27	21.25004739998974	29.01433301336791	28.39699926464957	21.338620321992778
28-29	20.960355414433852	28.885346504692226	28.996996144251046	21.157301936622876
30-31	21.61285573817726	29.306290448236748	28.249041550750604	20.831812262835385
32-33	20.648530920126753	29.197762939884512	28.926203805130633	21.2275023348581
34-35	20.815635410265713	28.690463800669512	28.738048415399746	21.755852373665018
36-37	21.417394549260944	29.345533630479903	28.21461776789498	21.022454052364175
38-39	21.21217476523653	28.955223347730197	28.477904430895506	21.354697456137767
40-41	21.305038291483537	29.002350827106287	28.14136863061577	21.5512422507944
42-43	21.0837959973252	29.047239900816013	28.6658084952523	21.20315560660649
44-45	21.59960315736842	28.525810025947678	28.508724543618413	21.365862273065492
46-47	21.380808266721825	29.05430109268627	28.64758313518686	20.91730750540505
48-49	21.212354485814238	28.725459032243933	28.791162868883664	21.271023613058166
50-51	21.49899833283917	28.7889133444285	28.435768859801946	21.276319462930378
52-53	20.89661266982065	28.884249471922608	28.600004738874834	21.61913311938191
54-55	21.375329981465022	28.879442308864412	28.26355324111996	21.481674468550604
56-57	21.51661931953375	28.741801202672402	28.265462842752648	21.476116635041205
58-59	21.197635470727448	28.275033373183646	28.757140815665004	21.770190340423902
60-61	21.696951864108378	28.79933258449099	28.165802162522812	21.33791338887782
62-63	21.07425883812853	28.87853130427316	28.639088861866764	21.40812099573155
64-65	21.099639748841035	28.392490941912857	28.659469839129496	21.84839947011661
66-67	21.317483712492646	28.668244282487176	28.32406840195253	21.690203603067648
68-69	21.791922292653048	28.467895348653855	28.30250289193184	21.437679466761253
70-71	21.75700854849332	28.67432048155429	27.995769526937273	21.57290144301512
72-73	21.43781142363434	28.705391451879404	28.55446584986474	21.30233127462152
74-75	21.74416937306436	28.242226154467424	28.19633744969259	21.817267022775628
76-77	21.579662214219788	28.74689047875526	28.296448425927213	21.376998881097737
78-79	21.63898524057338	28.467077927718005	28.453887222370206	21.440049609338406
80-81	21.73875287164452	28.373702599595784	28.227819040795943	21.65972548796375
82-83	21.15668270433923	28.624448942630554	28.621559424130794	21.597308928899427
84-85	21.417452608012656	28.23612781345502	28.18992608912248	22.156493489409844
86-87	21.6555420061817	28.510646318596226	28.21165879677297	21.622152878449103
88-89	21.42890181841263	28.065032585843603	28.609351055026405	21.896714540717365
90-91	21.75843026975619	28.16493588231328	28.255921236378473	21.82071261155206
92-93	21.270074240081165	28.46640694356186	28.5664522566206	21.69706655973637
94-95	21.216122522651272	28.03722220780355	28.461041427705187	22.285613841839996
96-97	21.47540795719201	28.223636285798754	28.444566396846977	21.85638936016226
98-99	21.4362231092495	28.01846053957795	28.45299950933989	22.092316841832666
100	21.564285658957935	27.821044368115633	28.277715043560526	22.336954929365913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	296.0
1	359.0
2	362.0
3	311.5
4	367.5
5	491.0
6	714.5
7	1128.0
8	1675.0
9	2363.0
10	3405.5
11	4429.5
12	5266.0
13	6258.0
14	7135.0
15	8087.5
16	9125.0
17	9962.0
18	10853.0
19	11790.5
20	12783.5
21	14068.5
22	15800.5
23	17802.0
24	20080.0
25	23492.0
26	28300.5
27	34308.0
28	41785.5
29	50523.5
30	60287.0
31	70091.0
32	79737.5
33	89202.5
34	97635.5
35	104925.5
36	110635.0
37	115195.0
38	118300.0
39	119584.5
40	119389.0
41	117476.0
42	114057.5
43	109672.0
44	104501.5
45	98573.0
46	92933.5
47	88364.0
48	84209.0
49	81519.0
50	80304.5
51	75593.0
52	67235.5
53	59484.0
54	53313.0
55	48211.5
56	43553.5
57	38952.0
58	34629.5
59	30295.0
60	25874.0
61	21743.0
62	17949.0
63	14619.0
64	11647.5
65	9240.5
66	7332.5
67	5700.5
68	4380.5
69	3371.0
70	2618.0
71	1982.5
72	1477.0
73	1136.5
74	851.5
75	631.5
76	458.5
77	309.0
78	227.0
79	157.0
80	112.5
81	77.0
82	48.5
83	37.0
84	27.0
85	18.5
86	15.0
87	10.0
88	7.5
89	7.0
90	6.0
91	3.0
92	1.5
93	3.0
94	2.5
95	1.0
96	0.5
97	0.5
98	0.5
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.960913471611146
2	25.204106432013408
3	6.233731405824429
4	24.09976115650534
5	12.727123402472241
6	10.283645505971087
7	8.2959983239053
8	9.272993924156715
9	5.632784412319296
10-11	6.979610307982401
12-13	10.420498638173056
14-15	7.69424680494448
16-17	8.120276555625393
18-19	6.857087785459878
20-21	8.086050701864655
22-23	5.746859417557092
24-25	5.224286612193589
26-27	9.830328933584747
28-29	6.504688874921433
30-31	4.133148962916405
32-33	8.65267127592709
34-35	7.397527760318458
36-37	7.249193379425938
38-39	6.106599622878693
40-41	8.367701655143515
42-43	6.4074418604651155
44-45	6.608305049235281
46-47	5.8387094070815
48-49	3.980942803268385
50-51	7.064638592080453
52-53	4.503582652419862
54-55	4.850886235072282
56-57	4.903448564843914
58-59	3.823339618688456
60-61	4.188074586214121
62-63	3.183138487324534
64-65	4.5617766603813115
66-67	2.3491975696626857
68-69	5.39868426566101
70-71	2.5517368531322018
72-73	1.6835700817096166
74-75	0.7609972763461136
76-77	1.103272574900482
78-79	1.777716320972135
80-81	1.950622250157134
82-83	0.8097045883092395
84-85	1.251958935679866
86-87	0.4055478734548502
88-89	0.7125413785878902
90-91	0.32078776450869473
92-93	0.3510412738319715
94-95	0.5462057406243453
96-97	0.5556589147286821
98-99	0.45777498428661223
100	0.4628703121726378
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2983125.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.36171093250113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.76200359979225	94.20513931065314
2	1.8733920265404644	3.6104652184948938
3	0.20782015667368683	0.6007771759001072
4	0.06395195503188654	0.24650079217403845
5	0.031085406530237794	0.14977214791430285
6	0.014758621943512148	0.0853299636889671
7	0.009931645494679198	0.06699212465696477
8	0.009124653472041245	0.0703413776185665
9	0.00458062512505119	0.03972571867713011
>10	0.02111376007773071	0.32421287201372706
>50	9.054079682229141E-4	0.057553884680645886
>100	0.001017445639093802	0.21564985452291177
>500	2.447633310435971E-4	0.16944722184563737
>1k	6.993238014615455E-5	0.15809233715892124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.352189398701027E-5	0.0	0.0	0.0
3	0.0	3.352189398701027E-5	0.0	0.0	0.0
4	0.0	3.352189398701027E-5	0.0	0.0	0.0
5	0.0	6.704378797402053E-5	0.0	0.0	0.0
6	0.0	6.704378797402053E-5	0.0	0.0	0.0
7	0.0	6.704378797402053E-5	0.0	3.352189398701027E-5	0.0
8	0.0	6.704378797402053E-5	0.0	3.352189398701027E-5	0.0
9	0.0	6.704378797402053E-5	0.0	6.704378797402053E-5	0.0
10-11	0.0	6.704378797402053E-5	0.0	8.380473496752567E-5	0.0
12-13	0.0	6.704378797402053E-5	0.0	1.005656819610308E-4	0.0
14-15	0.0	6.704378797402053E-5	0.0	1.1732662895453594E-4	0.0
16-17	5.02828409805154E-5	6.704378797402053E-5	0.0	1.5084852294154622E-4	0.0
18-19	8.380473496752567E-5	6.704378797402053E-5	0.0	2.1789231091556675E-4	0.0
20-21	1.005656819610308E-4	6.704378797402053E-5	0.0	2.3465325790907187E-4	0.0
22-23	1.005656819610308E-4	6.704378797402053E-5	0.0	2.51414204902577E-4	0.0
24-25	1.1732662895453594E-4	1.005656819610308E-4	0.0	3.184579928765975E-4	0.0
26-27	1.3408757594804107E-4	1.005656819610308E-4	0.0	4.525455688246386E-4	0.0
28-29	1.3408757594804107E-4	1.005656819610308E-4	0.0	7.877645086947413E-4	0.0
30-31	1.3408757594804107E-4	1.005656819610308E-4	0.0	0.002011313639220616	0.0
32-33	1.3408757594804107E-4	1.005656819610308E-4	0.0	0.005933375235700818	0.0
34-35	1.3408757594804107E-4	1.005656819610308E-4	0.0	0.012587471192122356	0.0
36-37	1.3408757594804107E-4	1.005656819610308E-4	0.0	0.02321391158600461	0.0
38-39	1.3408757594804107E-4	1.005656819610308E-4	0.0	0.04000838047349675	0.0
40-41	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.05993714644877436	0.0
42-43	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.08026817515189608	0.0
44-45	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.10552692227110833	0.0
46-47	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.13539492981353446	0.0
48-49	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.1675256652000838	0.0
50-51	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.2030421118793212	3.352189398701027E-5
52-53	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.2406704378797402	3.352189398701027E-5
54-55	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.2815001047559187	5.02828409805154E-5
56-57	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.3317494238424471	6.704378797402053E-5
58-59	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.3878483134297088	6.704378797402053E-5
60-61	1.6760946993505134E-4	1.005656819610308E-4	0.0	0.4470815001047559	6.704378797402053E-5
62-63	1.8437041692855646E-4	1.005656819610308E-4	0.0	0.5098680075424261	6.704378797402053E-5
64-65	2.1789231091556675E-4	1.005656819610308E-4	0.0	0.5797779174523361	6.704378797402053E-5
66-67	2.3465325790907187E-4	1.005656819610308E-4	0.0	0.666180599203855	6.704378797402053E-5
68-69	2.3465325790907187E-4	1.005656819610308E-4	0.0	0.7612989733919966	6.704378797402053E-5
70-71	2.3465325790907187E-4	1.005656819610308E-4	0.0	0.873396186884559	6.704378797402053E-5
72-73	2.3465325790907187E-4	1.005656819610308E-4	0.0	0.9904881625811859	6.704378797402053E-5
74-75	2.3465325790907187E-4	1.5084852294154622E-4	0.0	1.1156756756756758	6.704378797402053E-5
76-77	2.3465325790907187E-4	2.011313639220616E-4	0.0	1.2530986800754242	6.704378797402053E-5
78-79	2.3465325790907187E-4	2.011313639220616E-4	0.0	1.410701864655353	6.704378797402053E-5
80-81	2.3465325790907187E-4	2.1789231091556675E-4	0.0	1.5885019903624555	6.704378797402053E-5
82-83	2.3465325790907187E-4	2.51414204902577E-4	0.0	1.7821579719254137	6.704378797402053E-5
84-85	2.3465325790907187E-4	2.6817515189608214E-4	0.0	1.9841944269851246	6.704378797402053E-5
86-87	2.3465325790907187E-4	2.6817515189608214E-4	0.0	2.2013827781269644	6.704378797402053E-5
88	2.3465325790907187E-4	2.6817515189608214E-4	0.0	2.386188979677352	6.704378797402053E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGACC	210	1.1494474E-5	19.248405	8
GTACCGT	170	0.0011884407	17.819426	6
GTATACG	250	2.0547287E-4	17.281494	1
GTACATG	10950	0.0	16.458565	1
GTATAGC	680	0.0	16.337545	1
TGTACCG	280	7.7242075E-6	16.179823	5
TACATGG	11210	0.0	15.523195	2
GTATATA	2185	0.0	15.253363	1
GTATAGG	895	0.0	15.171303	1
TATACTG	1405	0.0	15.047427	5
TAGACCG	235	6.058521E-4	14.994068	5
TACCGTG	270	1.0420123E-4	14.885127	7
ACATGGG	11000	0.0	14.751111	3
GTCCTAC	590	2.5993359E-9	14.645333	1
CATGGGG	3400	0.0	14.153004	4
GTGTAGC	885	0.0	13.947937	1
ATTATAC	1455	0.0	13.843914	3
GTACATA	1520	0.0	13.805704	1
GGATACG	270	0.006802263	13.71547	1
GTCTCAC	1675	0.0	13.633588	1
>>END_MODULE